Red Tide USB650 Fiber Optic Spectrometer Installation and
Contents
1. Product Related Documentation You can access documentation for Ocean Optics products by visiting our website at http www oceanoptics com Select Technical Operating Instructions then choose the appropriate document from the available drop down lists Or use the Search by Model Number field at the bottom of the web page e Detailed instructions for SpectraSuite Spectrometer Operating Software are located at http www oceanoptics com technical SpectraSuite pdf e Detailed instructions for external triggering are located at http www oceanoptics com technical external triggering pdf e Detailed instructions for the Pasco Xplorer GLX are located at ftp ftp pasco com manuals English PS PS 2002 012 08950F 012 08950F pdf 170 00000 RT 02 1106 ili cean D CS me About This Manual 0606060600 8957X 7 Engineering level documentation is located on our website at Technical Engineering Docs You can also access operating instructions for Ocean Optics products from the Software and Technical Resources CD that ships vvith the product Upgrades Occasionally you may find that you need Ocean Optics to make a change or an upgrade to your system To facilitate these changes you must first contact Customer Support and obtain a Return Merchandise Authorization RMA number Please contact Ocean Optics for specific instructions vvhen returning a product V 170 00000 RT 02 1106 Chapter 1 Introduction P2. Red Tide USB650 Fiber Optic Spectrometer Installation and Operation Manual Document Number 170 00000 RT 02 1106 Offices Ocean Optics Inc World Headguarters 830 Douglas Ave Dunedin FL USA 34698 Phone 727 733 2447 Fax 727 733 3962 S a m 8 p m Mon Thu 8 a m 6 p m Fri EST E mail Info OceanOptics com General sales inquiries Orders OceanOptics com Questions about orders TechSupport OceanOptics com Technical support HALMA GROUP COMPANY Additional Offices 137 Xianxia Road Suite 1802 Changning District Shanghai PRC 200051 Phone 86 21 5206 8686 Fax 86 21 5206 8686 E Mail Sun Ling 6 OceanOptics com Geograaf 24 6921 EVV DUIVEN The Netherlands Phone 31 0 26 3190500 Fax 31 0 26 3190505 E Mail Info 9 OceanOpticsBV com Copyright 2001 2006 Ocean Optics Inc All rights reserved No part of this publication may be reproduced stored in a retrieval system or transmitted by any means electronic mechanical photocopying recording or othervvise vvithout vvritten permission from Ocean Optics Inc This manual is sold as part of an order and subiect to the condition that it shall not by vvay of trade or othervvise be lent re sold hired out or otherwise circulated without the prior consent of Ocean Optics Inc in any form of binding or cover other than that in which it is published Trademarks All products and services herein are the trademarks service marks register
3. Systems with more than five channels reguire a powered USB hub gt Procedure Follow the steps below to connect the Red Tide to a computer via the USB port 1 Install SpectraSuite on the destination computer See the SpectraSuite Spectrometer Operating Software Installation and Operation Manual see Product Related Documentation 2 Locate the USB cable USB CBL 1 provided with the Red Tide Insert the sguare end of the cable into the side of the Red Tide 4 Insert the rectangular end of the cable into the USB port of the computer 170 00000 RT 02 1106 7 cean pticSm If you installed SpectraSuite prior to connecting the Red Tide the SpectraSuite installs the Red Tide drivers If the drivers do not successfully install or if you connected the Red Tide to the computer before installing SpectraSuite consult Chapter 3 Troubleshooting 2 Installing the Red Tide If you have followed the previous steps and started SpectraSuite the spectrometer is already acquiring data Even with no light in the spectrometer there should be a dynamic trace displayed in the bottom of the graph If you allow light into the spectrometer the graph trace should rise with increasing light intensity This means the software and hardware are correctly installed Note the spectrometer s that you have installed are listed in the Data Sources pane of your SpectraSuite software Once you install the software and hardware and establis
4. UV4 The detector s standard window is replaced with a guartz window to enhance 10 Detector f Upgrade spectrometer performance lt 340 nm This upgrade is optional Red Tide Specifications The following sections provide specification information for the CCD detector in the Red Tide as well as the Red Tide Spectrometer itself CCD Detector Specifications Specification Value Detector Sony ILX511 CCD No of elements 2048 pixels Sensitivity 75 photons per count at 400 nm Pixel size 14 um x 200 um Pixel well depth 62 500 electrons Signal to noise ratio 250 1 at full signal A D resolution 12 bit Dark noise 3 2 RMS counts Corrected linearity gt 99 8 Red Tide Spectrometer Specification Value Dimensions 89 1 mm x 63 3 mm x 34 4 mm Weight 190 g Power consumption 90 mA Q 5 VDC 170 00000 RT 02 1106 21 D CS mc Specification Value Detector 2048 element linear silicon CCD array Detector range 200 1100 nm Entrance aperture 25 um wide slit Order sorting filters Installed longpass and bandpass filters Focal length 42 mm input 68 mm output Optical resolution 2 0 nm FWHM Stray light lt 0 05 at 600 nm lt 0 10 at 435 nm Dynamic range 2 x 10 system 1300 1 for a single acquisition Fiber optic connector SMA 905 to single strand optica
5. Ger can power other peripherals Ensure that peripherals comply with b p USB specifications 2 RS232 Tx Output 7 transmit signal Communicates with a computer over DB9 3 RS232 Rx Input 77 receive signal Communicates with a computer over DB9 4 Lamp Output TTL signal driven Active HIGH when the Lamp Enable command Enable p is sent to the spectrometer 5 Continuous Output TTL output signal used to pulse a strobe Divided down from the Strobe p master clock signal 6 Ground Input Output Ground 7 External input TTL input trigger signal See External Triggering Options Trigger In p document for info Single TTL output pulse used as a strobe signal Has a programmable 8 9 Output delay relative to the beginning of the spectrometer integration Strobe period 9 DC SCL Input Output The DC clock signal for communications to other 1 C peripherals 10 PC SDA Input Output The I C Data signal for communications to other DC peripherals 24 170 00000 RT 02 1106 Number 10 pin accessory connector diagram 23 pin definitions 24 A accessories 5 8 Accessories 3 accessory connector pinout 23 Adobe Acrobat Reader 4 C Calibrating iii 13 calibration 13 preparing for 14 procedure 14 calibration coefficients saving in USB mode 16 CCD 21 CCD Detector 20 collimating mirror 20 Components Table 20 D detector 21 Detector Collection Lens 20 document audience iii purpose iii summary il
6. Overview How Sampling Works Ocean Optics components function in a sampling system as follows 1 The user stores reference and dark measurements to correct for instrument response variables 2 The light from the light source transmits through an optical fiber to the sample 3 The light interacts with the sample 4 Another optical fiber collects and transmits the result of the interaction to the spectrometer 5 The spectrometer measures the amount of light and transforms the data collected by the spectrometer into digital information 6 The spectrometer passes the sample information to SpectraSuite 7 SpectraSuite compares the sample to the reference measurement and displays processed spectral information Modular Light Sources and Sampling Accessories Ocean Optics offers a complete line of spectroscopic accessories for use with the Red Tide Most of our spectroscopic accessories have SMA connectors for application flexibility Accordingly changing the sampling system components is as easy as unscrewing a connector and replacing an accessory Available accessories include the following USB ISS UV VIS Integrated Sampling System for Cuvettes 200 1100 nm USB ISS VIS Integrated Sampling System for Cuvettes 390 900 nm USB LS 450 Pulsed Blue LED Module USB DT Deuterium Tungsten Light Source 200 2000 nm 170 00000 RT 02 1106 3 cean PLCS me 1 Introduction Shipment Components The following informati
7. The Welcome screen appears Click the Next button The Destination Location screen appears Accept the default installation location or click the Brovvse button to specify a directory Then click the Next button The Program Manager Group screen appears Click the Next button The Start Installation screen appears Click the Next button to begin the installation Once the installation finishes the Installation Complete screen appears Click the Finish button and reboot the computer when prompted Navigate to the USB EEPROM Programmer from the Start menu and run the software Click on the desired Red Tide device displayed in the left pane of the USB Programmer screen Double click on each of the calibration coefficients displayed in the right pane of the USB Programmer screen and enter the new values acquired in Steps 5 and 6 of the Calibrating the Wavelength of the Spectrometer section in this appendix Repeat Step 12 for all of the new values Click on the Save All Values button to save the information and then Exit the USB Programmer software The new wavelength calibration coefficients are now loaded onto the EEPROM memory chip on the Red Tide 16 170 00000 RT 02 1106 Appendix B External Triggering Overview The Red Tide supports three triggering modes which are set with the Trigger Mode command A detail of each triggering mode follows Normal Free Running In this mode the Red Tide uses the user defined
8. 607 Standard Error 0 125540214 Observations 22 Coefficients Intercept 190 473993 X Variable 1 0 36263983 X Variable 2 1 174416E X Variable 3 2 523787E Third coefficient 170 00000 RT 02 1106 Intercept Standard Error 0 36904753 First coefficient 001684745 0 8 35279E 07 2 656608 1 Second coefficient 15 A Calibrating the Wavelength of the Red Tide 7 cean pticSm Record the Intercept as well as the First Second and Third Coefficients Additionally look at the value for R squared It should be very close to 1 If not you have most likely assigned one of your wavelengths incorrectly Keep these values at hand Saving the New Calibration Coefficients USB Mode Ocean Optics programs wavelength calibration coefficients unique to each Red Tide onto an EEPROM memory chip in the Red Tide You can overwrite old calibration coefficients on the EEPROM if you are using the Red Tide via the USB port gt Procedure To save wavelength calibration coefficients using the USB mode perform the following steps 1 a po p p 10 11 12 13 14 Ensure that the Red Tide is connected to the computer and that you have closed all other applications Point your browser to http www oceanoptics com technical softwaredownloads asp and scroll down to Microcode Select USB EEPROM Programmer Save the setup file to your computer Run the Setup exe file to install the software
9. Pixel Pixel Pixel Difference 253 65 175 30625 5359375 253 56 0 09 296 73 296 87616 25934336 296 72 0 01 302 15 312 97344 30371328 302 40 0 25 313 16 342 116964 40001688 313 02 0 13 334 15 402 161604 64964808 334 19 0 05 365 02 490 240100 117649000 365 05 0 04 404 66 604 364816 220348864 404 67 0 01 407 78 613 375769 230346397 407 78 0 00 435 84 694 481636 334255384 435 65 0 19 546 07 1022 1044484 1067462648 546 13 0 06 576 96 1116 1245456 1389928896 577 05 0 09 579 07 1122 1258884 1412467848 579 01 0 06 696 54 1491 2223081 3314613771 696 70 0 15 706 72 1523 2319529 3532642667 706 62 0 10 727 29 1590 2528100 4019679000 727 24 0 06 738 40 1627 2647129 4306878883 738 53 0 13 751 47 1669 2785561 4649101309 751 27 0 19 5 Use the spreadsheet or calculator to calculate the wavelength calibration coefficients In the spreadsheet program find the functions to perform linear regressions using Quattro Pro look under Tools Advanced Math If using Excel look under Analysis ToolPak 6 Select the true wavelength as the dependent variable Y Select the pixel number pixel number squared and the pixel number cubed as the independent variables X After executing the regression you will obtain an output similar to the one shown below Numbers of importance are noted Regression Statistics Multiple R 0 999999831 R Square 0 999999663 ___ R Squared Adjusted R Square 0 999999
10. ate passwords for the other software applications on the back of the Software and Technical Resources CD package Additional Equipment Needed SpectraSuite Operating Software SpectraSuite is the only spectrometer operating software that works with the Red Tide See About SpectraSuite for more information The Red Tide can also be used with the Pasco Xplorer GLX 4 170 00000 RT 02 1106 cean PLCS me eg 1 Introduction Other Accessories Available Visit us at www OceanOptics com for a complete list of products available for all of your spectroscopy needs Fibers Light Sources Integrated Sampling Systems Cuvettes Filter Holders Lithium Ion Battery Pack UUUUUU 170 00000 RT 02 1106 5 cean DCS 1 Introduction Ayat in Photonics Chapter 2 Installing the Red Tide Overview You must install the SpectraSuite software application prior to connecting the Red Tide Spectrometer to the computer The SpectraSuite software installation installs the drivers reguired for Red Tide installation If you do not install SpectraSuite first the system will not properly recognize the Red Tide If you have already connected the Red Tide to a computer running on a Windows platform prior to installing SpectraSuite consult Chapter 3 Troubleshooting for information on correcting a corrupt Red Tide installation Red Tide Installation Note The USB port on a computer can power up to five Red Tide spectrometer channels
11. ea acea A ce ee aa poat 8 Chapter 3 Troubleshooting nni 9 EEN 9 Red Tide Connected to Computer Prior to SpectraSuite Installation 9 Windows Operating Systems mean nana aa aaa amana amana 9 Remove the Unknown Device fromWindowsDeviceManager 9 Remove Improperly Installed Eiles mm ne m m m 10 Mac Operating Systems nenea nana aaa m aa ana nana nana ana 10 Linux Operating Systems AA 11 170 00000 RT 02 1106 cean pticSm Table of Contents Appendix A Calibrating the Wavelength of the Red Tide 13 EEN 13 About Wavelength Calibration EE 13 Calibrating the Spectrometer E 14 Preparing for Calbratton ttnn ttnn 14 14 Calibrating the Wavelength of the Spectrometer Saving the New Calibration Coefficients USB Mode 16 Appendix B External Triggering 17 aha gece tte a anama eege 17 Normal Free Running kasap n d bam yash la lemek eek biy ta tt elena di esli 17 External Software alyan aya celal ele ade bra adadan eyle 17 External Hardware Trigger 17 5 5 19 5 66 Dan 19 How the Red Tide Works 19 Red Tide Components nenea amana ana ana na a a naaeena 20 Red Tide Sp
12. ecifica iONS ns ceas em aan a ar a a e i a a al dana 21 CCD Detector 5 ray anaae ar n 21 Red RRE de Lu EE 21 10 Pin Accessory Connector Pinot 23 10 Pin Accessory Connector Pinout Diagram mm nenea neam na amana 23 10 Pin Accessory Connector Pin Definitions and 24 AEP MEAN EM NEM EREL EE R Una 25 170 00000 RT 02 1106 About This Manual Document Purpose and Intended Audience This document provides the users of Red Tide Spectrometers with instructions for setting up calibrating and performing experiments with their spectrometer Document Summary Chapter Description Chapter 1 Introduction Contains descriptive information about the Red Tide Spectrometer and how sampling works It also provides a list of system reguiremenis interface options and shipment componenis Chapter 2 Installing the Red Tide Provides installation instructions Chapter 3 Troubleshooting Contains recommended steps to isolate and correct common problems Appendix A Calibrating the Provides instructions for calibrating the Red Tide Wavelength of the Red Tide Spectrometer Appendix B External Triggering Contains information about external triggering for the Red Tide Appendix C Specifications Contains technical specifications and connector pinouts for the Red Tide Spectrometer
13. ed trademarks or registered service marks of their respective owners Limit of Liability Every effort has been made to make this manual as complete and as accurate as possible but no warranty or fitness is implied The information provided is on an as is basis Ocean Optics Inc shall have neither liability nor responsibility to any person or entity with respect to any loss or damages arising from the information contained in this manual Table of Contents About ul EE ul UE ii Document Purpose and Intended Audence AAA iii Beleg TuS EE ii Product RelatedDocumentatioenn DAAAAAA I gt gt gt gt mm aaa ae ii Uporades ee de aa a al iu d a la i a Ba aa la pap GR ed a d d IV Chapter 1 Introduction sisme inene dada ecua s 1 EEN Sote ci rea arda dna lea to Canto a ai a z 1 STI 2 EE Ee ill 2 EEPROMUlilzation amana 2 About Spectra Suites saca Delete A yaka dak aka lama a Bid ee Kaan ma ENEE di 3 Sampling System COvernlew 1 3 How Sampling Works nenea aaa amana nana nana aaa 3 Modular Light SourcesandSamplingAccessories nenea nea ana ini 3 Shipment Components sea Ke 4 Additional Equipment Needed EE A Other Accessories Available EE 5 Chapter 2 Installing the Red Tide 7 ET EE H Red We CHE UE e EE 7 Connect Spectroscopic Accessories EEN 8 External Triggering OpIIONS iaca ac
14. g page for an explanation of the function of each numbered component in the Red Tide Spectrometer in this diagram 170 00000 RT 02 1106 19 C Specifications Red Tide Components Table Ocean Optics permanently secures all components in the Red Tide at the time of manufacture Only Ocean Optics technicians can replace interchangeable components where noted cean PtiCS inc Item Name Description 1 SMA 905 Secures the input fiber to the spectrometer Light from the input fiber enters the Connector optical bench through this connector A dark piece of material containing a rectangular aperture which is mounted directly behind the SMA Connector The size of the aperture from 5 um to 200 um regulates the amount of light that enters the optical bench and controls spectral resolution 2 Slit You can also use the Red Tide without a Slit In this configuration the diameter of the fiber connected to the Red Tide determines the size of the entrance aperture Only Ocean Optics technicians can change the Slit Restricts optical radiation to pre determined wavelength regions Light passes through the Filter before entering the optical bench Both bandpass and longpass 3 Filter filters are available to restrict radiation to certain wavelength regions Only Ocean Optics technicians can change the Filter Focuses light entering the optical bench towards the Grating of the spectrometer i Collimating Specify standard o
15. gth of your spectrometer Though each spectrometer is calibrated before it leaves Ocean Optics the wavelength for all spectrometers will drift slightly as a function of time and environmental conditions Ocean Optics recommends periodically recalibrating the Red Tide About Wavelength Calibration You are going to be solving the following eguation which shows that the relationship between pixel number and wavelength is a third order polynomial Ap gt 14 Cip Cp Where A the wavelength of pixel p 1 the vvavelength of pixel 0 C the first coefficient nm pixel C the second coefficient nm pixel C the third coefficient nm pixel R the reference intensity at wavelength A You will be calculating the value for and the three Cs 170 00000 RT 02 1106 13 A Calibrating the Wavelength of the Red Tide cean pticSm Calibrating the S pectrometer Preparing for Calibration To recalibrate the wavelength of your spectrometer you need the following components A light source capable of producing spectral lines Note Ocean Optics HG 1 Mercury Argon lamp is ideal for recalibration If you do not have an HG I you need a light source that produces several at least 4 6 spectral lines in the wavelength region of your spectrometer A Red Tide Spectrometer An optical fiber for spectrometers without a built in slit a 50 um fiber works best A spreadsheet program Excel or Quat
16. h your sampling system you are ready to take measurements Connect Spectroscopic Accessories To find operating instructions for Red Tide compatible products such as light sources sampling chambers and probes consult the Software and Technical Resources CD or the Ocean Optics website at http www oceanoptics com technical operatinginstructions asp External Triggering Options You can trigger the Red Tide using a variety of External Triggering options through the 10 pin Accessory Connector on the spectrometer See Appendix B External Triggering for more information 8 170 00000 RT 02 1106 Chapter 3 Troubleshooting Overview The following sections contain information on troubleshooting issues you may encounter when using the Red Tide Spectrometer Red Tide Connected to Computer Prior to SpectraSuite Installation Windows Operating Systems If you connected your Ocean Optics Red Tide device to the computer prior to installing your Ocean Optics software application on a Windows platform you may encounter installation issues that you must correct before your Ocean Optics device will operate properly Follow the applicable steps below to remove the incorrectly installed device device driver and installation files Note If these procedures do not correct your device driver problem you must obtain the Correcting Device Driver Issues document from the Ocean Optics website http www oceanoptics com technical eng
17. he Red Tide EEPROM Utilization An EEPROM memory chip in each Red Tide contains wavelength calibration coefficients linearity coefficients and a serial number unigue to each individual spectrometer The SpectraSuite software application reads these values directly from the spectrometer enabling the ability to hot swap spectrometers between computers without entering the spectrometer coefficients manually on each computer 2 170 00000 RT 02 1106 cean D CS me About SpectraSuite 1 Introduction SpectraSuite is the latest generation of operating software for all Ocean Optics spectrometers It is a completely modular Java based spectroscopy software platform that operates on Windows Macintosh and Linux operating systems The software can control any Ocean Optics USB spectrometer and device as well as any other manufacturer s USB instrumentation using the appropriate drivers SpectraSuite is a user customizable advanced acquisition and display program that provides a real time interface to a variety of signal processing functions With SpectraSuite you have the ability to perform spectroscopic measurements such as absorbance reflectance and emission control all system parameters collect and display data in real time and perform reference monitoring and time acquisition experiments Consult the SpectraSuite manual for hardware requirements when using SpectraSuite see Product Related Documentation Sampling System
18. i EEPROM 2 external triggering 8 17 170 00000 RT 02 1106 Index filter 20 focusing mirror 20 G grating 20 Installation 7 installed filter 20 L L2 Detector Collection Lens 20 Lens 20 light sources 3 M memory chip 2 mirror 20 packing list 4 passwords 4 product related documentation iii R Red Tide specifications 21 25 cean ptics mc ZA First in Photonics Index Sampling triggering 8 17 Accessories 3 troubleshooting 9 System 3 Linux systems 11 setup 7 Mac systems 10 shipment components 4 Windows systems 9 slit 20 SMA Connector 20 U Software and Resources Library CD 4 specifications 19 detector 21 upgrades iv Red Tide 21 SpectraSuite 3 VI spectroscopic accessories 8 Wavelength Calibration about 13 Wavelength Calibration Data File 4 Wavelength Calibration Data Sheet 4 26 170 00000 RT 02 1106
19. ineering correctingdevicedriverissues pdf Remove the Unknown Device from Windows Device Manager gt Procedure 1 Open Windows Device Manager Consult the Windows operating instructions for your computer for directions if needed 2 Locate the Other Devices option and expand the Other Devices selection by clicking on the sign to the immediate left 170 00000 RT 02 1106 9 3 Troubleshooting cean PLCS in Note Improperly installed USB devices can also appear under the Universal Serial Bus Controller option Be sure to check this location if you cannot locate the unknown device Locate the unknown device marked with a large question mark Right click on the Unknown Device listing and select the Uninstall or Remove option Click the OK button to continue A warning box appears confirming the removal of the Unknown Device Click the OK button to confirm the device removal Disconnect the Red Tide from your computer Locate the section in this chapter that is appropriate to your operating system and perform the steps in the following Remove Improperly Installed Files section Remove Improperly Installed Files gt Procedure 1 2 4 5 6 7 Open Windows Explorer Navigate to the Windows INF directory Note If the INF directory is not visible you must disable the Hide System Files and Folders and Hide File Extensions for Known File Types options in Windows Folder Options Access Wi
20. integration clock and continuously scans the CCD array External Software Trigger In this mode the Red Tide uses the user defined integration clock however the A D converter is required to wait until the Trigger Input Signal goes HIGH before it acquires the data at the start of the next integration period This is an asynchronous trigger mode that allows the user to define an integration period External Hardware Trigger In this mode the Red Tide uses an internally generated clock to generate the integration period On the rising edge of this signal the internal logic resets the CCD array integrates for 50ms This is a synchronous trigger mode but the integration time is fixed OEMs can contact Ocean Optics for other periods 170 00000 RT 02 1106 17 cean DCS B External Triggering O 18 170 00000 RT 02 1106 Appendix C Specifications Overview This appendix contains information on spectrometer operation specifications and system compatibility It also includes accessory connector pinout diagrams and pin specific information How the Red Tide Works Below is a diagram of how light moves through the optical bench of an Red Tide Spectrometer The optical bench has no moving parts that can wear or break all the components are fixed in place at the time of manufacture Items with an asterisk are user specified Red Tide Spectrometer with Components See Red Tide Components Table on the followin
21. l fiber 0 22 NA Data transfer rate Full scans into memory every 13 milliseconds with USB 2 0 port Integration time 10 microseconds to gt 60 seconds detector s limit is 15 sec Interfaces USB 2 0 480 Mbps USB 1 1 compatible RS 232 2 wire 115 2 K baud Operating systems Windows 98 Me 2000 XP Mac OS X and Linux when using the USB port Analog channels No 22 170 00000 RT 02 1106 cean D CS me First in Photonics Ske ves C Specifications 10 Pin Accessory Connector Pinout The Red Tide features a 10 pin Accessory Connector located on the front of the unit as shown 10 Pin Connector Location of Red Tide 10 Pin Accessory Connector 10 Pin Accessory Connector Pinout Diagram When facing the 10 pin Accessory Connector on the front of the vertical wall of the Red Tide pin numbering is as follows Pin Orientation 1 O 10 8 6 15 SMA 9 4 3 USB Port Looking at Front of Red Tide 10 Pin Accessory Connector Pinout Diagram 170 00000 RT 02 1106 23 C Specifications 10 Pin Accessory Connector Pin Definitions and Descriptions cean PICS nc The following table contains information regarding the function of each pin in the Red Tide s 10 Pin Accessory Connector 7 Function Input Output Description Van V inputot Input power pin for Red Tide When operating via USB this pin 1 or EV Se
22. led by our SpectraSuite software a completely modular Java based spectroscopy software platform that operates on Windows Macintosh and Linux operating systems Ocean Optics Red Tide Fiber Optic Spectrometer 170 00000 RT 02 1106 1 cean pticSm 1 Introduction Features Sony ILX511 linear silicon CCD array detector Responsive from 350 to 1000 nm Sensitivity of up to 75 photons count at 400 nm An optical resolution of 2 0 FWHM Integration times from 3 ms to 65 seconds 15 seconds typical maximum Embedded microcontroller allows programmatic control of all operating parameters EEPROM storage for Wavelength Calibration Coefficients Oooooco Linearity Correction Coefficients Other configuration parameters Low power consumption of only 450 mW 12 bit 1 2 A D Converter 3 triggering modes 2 programmable strobe signals for triggering other devices 24 pin connector for interfacing to external products Programmable for Standalone Operation CE Certification UUUUUUU System Requirements You can use the Red Tide s USB connectivity with any computer that meets the following requirements e Operating system is one of the following e Windows 98 Me 2000 XP Apple Macintosh OS X version 10 0 or later Linux Red Hat 9 or later Fedora any version Debian 3 1 Sarge and SUSE 9 0 or later e Ocean Optics SpectraSuite software application installed and configured for use with t
23. ndows Folder Options from Windows Explorer under the Tools Folder Options menu selection Delete the OOI_USB INF in the INF directory If your computer is running either the Windows 2000 or XP operating system you must also delete the OOL USB PNF file in the INF directory Navigate to the Windows System32 Drivers directory Delete the EZUSB SYS file Reinstall your Ocean Optics application and reboot the system when prompted Plug in the USB device The system is now able to locate and install the correct drivers for the USB device Mac Operating Systems Since there are no device files for the Red Tide Spectrometer in a Mac operating system you should not encounter any problems if you installed the spectrometer before the SpectraSuite software 10 170 00000 RT 02 1106 cean ptics mc m First in Photonics Linux Operating Systems For Linux operating systems all you need to do is install the SpectraSuite software then unplug and replug in the spectrometer Technically the driver files for Linux simply give nonprivileged users permission to use newly connected hardware There isn t any long term harm to plugging in the device before installing the software 170 00000 RT 02 1106 3 Troubleshooting 11 cean DCS 3 Troubleshooting ooo 12 170 00000 RT 02 1106 Appendix A Calibrating the Wavelength of the Red Tide Overview This appendix describes how to calibrate the wavelen
24. on and documentation ships with the Red Tide Spectrometer Q Packing List The packing list is inside a plastic bag attached to the outside of the shipment box the invoice arrives separately It lists all items in the order including customized components in the spectrometer such as the grating detector collection lens and slit The packing list also includes the shipping and billing addresses as well as any items on back order Q USB Cable USB CBL 1 Use this cable to connect your spectrometer to a computer running on a Windows Mac or Linux operating system Wavelength Calibration Data Sheet Each spectrometer is shipped with a Wavelength Calibration Data Sheet that contains information unique to your spectrometer SpectraSuite Operating Software reads this calibration data from your spectrometer when it interfaces to a computer via the USB port Note Please save the Wavelength Calibration Data Sheet for future reference Q Software and Technical Resources CD Each order ships with the Ocean Optics Software and Resources CD This disc contains software operating instructions and product information for all Ocean Optics software spectrometers and spectroscopic accessories You need Adobe Acrobat Reader version 6 0 or higher to view these files Ocean Optics includes the Adobe Acrobat Reader on the Software and Technical Resources CD Ocean Optics software requires a password during the installation process You can loc
25. r SAG Mirror Light enters the spectrometer passes through the SMA Connector Slit and Filter and then reflects off the Collimating Mirror onto the Grating Diffracts light from the Collimating Mirror and directs the diffracted light onto the 5 Grating Focusing Mirror Only Ocean Optics technicians can change the Grating 6 Focusing Receives light reflected from the Grating and focuses first order spectra onto the Mirror detector plane An optional component that attaches to the Detector to increase light collection efficiency It focuses light from a tall slit onto the shorter Detector elements 5 The L4 Detector Collection Lens should be used vvith large diameter slits or in 7 Collection pa applications with low light levels It also improves efficiency by reducing the effects of stray light Only Ocean Optics technicians can add or remove the L4 Detection Collection Lens Collects the light received from the Focusing Mirror or L4 Detector Collection Lens 8 Detector and converts the optical signal to a digital signal Each pixel on the Detector UV or VIS responds to the wavelengih of light that strikes it creating a digital response The spectrometer then transmits the digital signal to the SpectraSuite application 20 170 00000 RT 02 1106 cean pticsi ltem Name Description 9 OFLV OFLV Variable Longpass Order sorting Filters block second and third order light Filters These filters are optional
26. roduct Overview The Ocean Optics Red Tide Spectrometer is a preconfigured off the shelf spectrometer where all of the optical bench options such as grating and entrance slit size are already selected The red Tide can be used with various Ocean optics spectrometer accessories light sources and sampling optics to create application specific systems for various absorbance reflection and emission applications The Red Tide is low cost and has a small footprint making it ideal as a general purpose instrument for budget conscious teaching and research labs It has a wavelength range of 350 1000 nm and uses a detector with 650 active pixels that s 650 data points in one full spectrum or one data point per nanometer Data programmed into a memory chip on each Red Tide includes wavelength calibration coefficients linearity coefficients and the serial number unique to each spectrometer Our spectrometer operating software simply reads these values from the spectrometer a feature that enables hot swapping of spectrometers among computers The Red Tide Spectrometer connects to a computer via the USB port When connected through a USB 2 0 or 1 1 the spectrometer draws power from the host computer eliminating the need for an external power supply The Red Tide also interfaces to Pasco s Xplorer GLX a unique combination of datalogger and lab analysis tool that eliminates the need for a computer The Red Tide like all USB devices can be control
27. tro Pro for example or a calculator that performs third order linear regressions Note If you are using Microsoft Excel choose Tools Add Ins and check AnalysisToolPak and AnalysisTookPak VBA Calibrating the Wavelength of the Spectrometer 14 gt Procedure Perform the steps below to calibrate the wavelength of the spectrometer 1 Place SpectraSuite into Scope mode and take a spectrum of your light source Adjust the integration time or the A D conversion frequency until there are several peaks on the screen that are not off scale Move the cursor to one of the peaks and position the cursor so that it is at the point of maximum intensity Record the pixel number that is displayed in the status bar or legend located beneath the graph Repeat this step for all of the peaks in your spectrum Use the spreadsheet program or calculator to create a table like the one shown in the following figure In the first column place the exact or true wavelength of the spectral lines that you used In the second column of this worksheet place the observed pixel number In the third column calculate the pixel number squared and in the fourth column calculate the pixel number cubed 170 00000 RT 02 1106 cean D CS me First in Photonics Independent Variable Dependent Variables A Calibrating the Wavelength of the Red Tide Values Computed from the Regression Output True Wavelength nm
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