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iEMS Incubator Shaker HT - User Manual

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1. Thermo Scientific EMS Incubator Shaker HT User Manual Rev 2 4 Cat no 1506400A 59 Notes Thermo Scientific EMS Incubator Shaker HT User Manual Rev 2 4 Cat no 1506400A 60 Thermo Fisher Scientific Oy Microplate Instrumentation Ratastie 2 P O Box 100 FI 01621 Vantaa Finland www thermo com 15064004
2. Orange O The slot has reached the set temperature and is ready for operation Red O The slot is in use shaking and or incubating Green O The processing protocol is complete and the heating of the slot is still on The orange control LED flashes when a slot is heating up to the programmed target temperature The LED turns to a continuous orange color when the slot reaches the target temperature However the continuous orange LED may falsely indicate that a slot is at the target temperature under the following conditions e f you remove the thermal microplate holder and then reinsert it into the slot during the initial preheating of the slot e lf the programmed target temperature is lower than the actual temperature of the slot Thermo Scientific EMS Incubator Shaker HT User Manual Rev 2 4 Cat no 1506400A 23 6 4 Chapter 6 Operation Normal operation A A A A 6 4 1 Placing the microplate Caution To minimize the possibility of splashing aerosols and evaporation during shaking and incubation it is recommended you use microplate sealers Open the cover of the thermal microplate holder and place the microplate into the thermal microplate holder so that well A1 is located in the upper left corner as shown in Fig 6 2 Caution Do not handle the thermal microplate holder from its cover since it will come off Use the handle Fig 6 2 Caution Do not place the microplate holder into the instrum
3. Thermo Scientific EMS Incubator Shaker HT User Manual Rev 2 4 Cat no 15064004 Thermo Scientific EMS Incubator Shaker HT Cat no 5112250 and 5112257 User Manual Rev 2 4 February 2009 Cat no 1506400A Copyright Copyright O 2009 Thermo Fisher Scientific Corporation All rights reserved Printed in Finland Reproduction of the accompanying user documentation in whole or in part is prohibited Trademarks 8 and Microtiter is a registered trademark and EMS a trademark of Thermo Fisher Scientific All other trademarks and registered trademarks are the property of their respective holders Disclaimer Thermo Fisher Scientific reserves the right to change its products and services at any time to incorporate technological developments This manual is subject to change without prior notice as part of a continuous product development Although this manual has been prepared with every precaution to ensure accuracy Thermo Fisher Scientific assumes no liability for any errors or omissions nor for any damages resulting from the application or use of this information This manual supersedes all previous editions No liability for consequential damages Thermo Fisher Scientific shall not be liable for any damages whatsoever arising out of the use or inability to use this product Contact information Thermo Fisher Scientific P O Box 100 FI 01621 Vantaa Finland Tel 358 9 329 100 Fax
4. Program the interval shaking time by dialing the number keys first minutes then seconds and finally hours o Note Check where the cursor underline is located before you enter a value 1 The value will be entered at the location of the cursor For example for an interval shaking time of zero SHAKE TIME 00 00 20 Press 00 00 Enter INT TIME 00 00 00 Thermo Scientific iEMS Incubator Shaker HT User Manual Rev 2 4 Cat no 1506400A 21 Note Chapter 6 Operation The slots within a module shake at the speed that is programmed into the most recently started processing protocol Incorrect shaking speed may result if you start a protocol within the same module that has a shaking speed different from the other slots already in use To minimize the likelihood of this occurring it is recommended you program all processing protocols within a module with the same shaking speed Caution Do not place the microplate holder in or remove it from the instrument while the instrument is shaking This may damage the shaking mechanism The shaking speeds corresponding to the values you can enter are shown in the table below Table 6 1 Speed settings of the shaker Value 0 1 2 3 4 5 Shake speed rpm 0 400 650 900 1150 1400 Caution Splashing may occur if you use shaking with wells that contain too much liquid for a given speed To avoid splashing and potential cross contamination from well
5. Do not operate the instrument in an environment where potentially damaging liquids or gases are present Thermo Scientific EMS Incubator Shaker HT User Manual Rev 2 4 Cat no 1506400A 39 Chapter 8 Troubleshooting Guide Never operate your instrument from a power outlet that has no ground connection Never use a mains supply cable other than the mains supply cable designed for your region 8 2 2 General Thermal microplate holders Microplate holders will be hot when touched after use Do not handle the thermal microplate holder from its cover since it will come off The covers of the thermal microplate holders are not spring loaded but you should take care not to trap your fingers when closing the cover of the holder Avoid bumping the microplate holder while inserting it into the slot Viewing programmed processing parameters You can only view programmed processing parameters while in the programming mode When you review the parameters ensure you do not unintentionally change the programmed values Refer to Section 6 7 Splashing from overfilled wells Splashing may occur if you use shaking with wells that contain too much liquid for a given speed To avoid splashing and potential cross contamination from well to well do not overfill wells for a given shaking speed setting When the well is filled to 50 of its nominal volume the shake speed setting should not exceed value 4 It is recommended to use the setting 3
6. Shaker HT Feedback Form nnnnnnnnnnnnnnnnnnn 56 DA A nisse fen neste T dive nn dhaisonsstucbevadicsceunscosssaateneetante 57 Table of Contents Table of Contents 1 Safety Symbols and MarkingS ooconnncccccooncccconononccnonnnnncnnnnnnnnnnnnnnonnnnannnnnrnnnnnnnrnnnnnrs 7 2 About the User Manual cional 8 3 Introduction to the iEMST Incubator Shaker HT erernnnnvvnnnnnnnvrnnnnnnnvrnnnnnnvnnnnnnnvnnn 9 3 1 intended Use ve ee STEG 9 3 2 Principle Of operation iii aaa 9 3 3 Advantages of using EMS Incubator Shaker HT sreeersnavrreensnarrerrsnsrreessnsrrerersnsrreesennr 9 4 Functional Description rnnnvnrnnnnnnnnnnnnnvnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnen 10 4 1 EMS Incubator Shaker HT front VIEW uasarvrnnrannvvvnrannvnvrernsnnvnrsvsnnrnessnsnnvnessennrnessennnne 10 4 2 EMS Incubator Shaker HT rear view rraassvvvnnannvvvnnnnsvnvnessnsvnvnssssnnvnssnsnnvnssssnnvnsssennnne 11 4 3 Keyboard nd display cocino sida a 12 4 4 Thermal microplate holder rrrrnnnnnvnvnnnnnnnnnnnnnvnnnnnnnnannnannnnnennnnnnennnnnnnnnnnnnsnennnnnnnnnennennnn 13 5 Installation AAEE EEE TEAT stedet 14 5 1 Installation check lista danske 14 5 2 Whatto do Upon delivery vicario 14 5 2 1 Howto unpack aa ar hats kaste bane 14 5 2 2 Checking delivery for completeness urrarrvrnavarvvnnanvrvvnransrvenersrsrvenssnsrreessnsrrenssnsvnneens 15 5 2 3 Checking for damage during transport
7. EEE ma Fig 4 3 IEMS Incubator Shaker HT keyboard Listed below are the functions of the keyboard keys stop Stops the ongoing operation temp Sets the incubation temperature ON or OFF Used for starting preincubation preheating of the thermal microplate holders to the temperature set in processing protocol parameters shake Starts shaking with the speed and time set in processing protocol parameters start Starts operation defined by processing protocol parameters 0 9 Are used for entering slot numbers and parameter values C Clears an incorrectly keyed numeric value A Enters a keyed value or continues the program TEMPERATURE 37 0 C INC TIME 00 30 00 Fig 44 EMS Incubator Shaker HT display The alphanumeric liquid crystal display LCD has two 2 lines with up to 20 characters per line Thermo Scientific EMS Incubator Shaker HT User Manual Rev 2 4 Cat no 1506400A 12 Chapter 5 Installation 4 4 Thermal microplate holder Detachable cover with heating element Thermal and sensor contacts Bottom with heating element Adjustment screws 5 Handle A1 Position of the A1 well on the microplate HODE Fig 4 5 Thermal microplate holder The thermal microplate holder is designed for holding the microplate during the incubation process ensuring accurate temperature control Note Adjust the screws Fig 4 5 item 4 with a screwdriver so that the I microplate is held firmly
8. The samples can be potentially infectious Dispose of all used microplates disposable gloves syringes disposable tips etc as biohazardous waste Only use a cloth dampened with a decontaminant to decontaminate the instrument The use of spraying may damage the instrument If local or laboratory regulations prescribe regular decontamination it is not advisable to use formaldehyde since even small traces of formaldehyde affect Thermo Scientific EMS Incubator Shaker HT User Manual Rev 2 4 Cat no 15064004 42 Chapter 8 Troubleshooting Guide the enzyme being used in ElA tests in a negative way resulting in bad test results Always use disposable gloves and protective clothing and operate in a well ventilated area Decontaminate the instrument prior to disposal See Section 7 4 and Appendix B on decontamination Service request protocol If the EMS Incubator Shaker HT requires service contact your local Thermo Fisher Scientific representative or the Thermo Fisher Scientific technical service department Do not under any circumstances send the instrument for service without any prior contact It is imperative to indicate the fault and nature of the required service This will ensure a faster return of the instrument to the customer Your local Thermo Fisher Scientific representative or distributor will take care of sending a complaint form that is the Warranty Claim Technical Sheet to the Thermo Fisher Scientific techni
9. To pause shaking Press shake PLACE NUMBER After pressing shake select the slot number by pressing number keys or by scrolling with the 4 key For example press 1 PLACE 1 900 rpm 00 00 20 PLACE 1 P t aaa SHAKE PAUSED To go on with shaking Press start and the program returns to the READY state Press C to return to the READY state if 09 29 15 you do not need to start a shake 6 7 Verifying incubation and shaking parameters You can display the parameters programmed for each slot To display the programmed parameters press the appropriate slot number and then press Enter Continue to press 41 to display the programmed parameters for temperature incubation time shake time interval time and shake speed slot while the slot is not heating or shaking When you review the parameters ensure you do not make any unintentional changes to the programmed values Caution You can only view programmed incubation and shaking parameters of a Thermo Scientific EMS Incubator Shaker HT User Manual Rev 2 4 Cat no 15064004 33 Chapter 6 Operation Warning To prevent electric shock turn the power off to the instrument and unplug the power cable during maintenance procedures 7 1 Regular and preventive maintenance 7 1 1 General Routine and service procedures must be performed by the user to prevent unnecessary wear or hazards and are described below at the frequency with which they should be applied For
10. in most cases Refer to Section 6 2 Splashing aerosols and evaporation To minimize the possibility of splashing aerosols and evaporation during shaking and incubation it is recommended you use microplate sealers Spills Wipe up all spills of liquids immediately from all surfaces of the instrument to prevent leakage into the electronics and mechanics of the instrument Clean and decontaminate the surfaces if necessary Refer to Section 7 1 2 Incubation time Do not remove the thermal microplate holder until the incubation time has elapsed If you remove the microplate holder from the slot before the incubation time has elapsed the incubation timer does not stop and continues to count down causing tracking of actual incubation time to be lost Refer to Section 6 4 3 6 During shaking Do not place the microplate holder in or remove it from the instrument while the instrument is shaking This may damage the shaking mechanism Thermo Scientific EMS Incubator Shaker HT User Manual Rev 2 4 Cat no 1506400A 40 Chapter 8 Troubleshooting Guide AN Do not reach into slot when power is turned on To prevent electric shock do not reach into a slot and or touch the power contacts at the rear of the slot when the power is turned on to the instrument If you need to access the slot for example for cleaning turn the power off to the instrument and unplug the power cable High temperatures gt The thermal microplate holder
11. in place during shaking A Warning Microplate holders will be hot when touched after use Thermo Scientific EMS Incubator Shaker HT User Manual Rev 2 4 Cat no 1506400A 13 Chapter 5 Installation 5 Installation 5 1 Installation check list The chapter on installation will contain an outline of the points mentioned in the check list below Table 5 1 Installation check list Tick Item Unpack the iEMS Incubator Shaker HT instrument carefully Refer to 5 2 1 Keep the original packaging and packing material for future transportation Check the delivery for completeness Refer to 5 2 2 Check for damage during transport Refer to 5 2 3 Place the instrument on a normal laboratory bench taking into account both the climatic and technical prerequisites Refer to 5 2 4 and 5 2 6 Leave sufficient clearance on both sides and at the rear of the unit Install the instrument Refer to 5 3 OO O OD O Connect the mains supply cable to the mains input socket Refer to 5 3 2 5 2 What to do upon delivery 5 2 1 How to unpack Move the unpacked instrument to its site of operation Unpack the EMS Incubator Shaker HT instrument and accessories carefully with the arrows on the transport package pointing upwards The following notes and instructions are sent with the instrument and are immediately available when you open the package e Warranty Certificate card e packing instructions packing list e transportation damage and
12. of the other slots In one module all slots have the same incubation temperature and they shake atthe same speed The slots should be preheated to the programmed target temperature before protocols are started 3 3 Advantages of using EMS Incubator Shaker HT The EMS Incubator Shaker HT provides several advantages relating mainly to the principle of operation in that it has e Extended temperature range e Exceptional temperature uniformity across the plate e Efficient orbital shaking e Capacity of up to three 3 microplates Thermo Scientific iEMS Incubator Shaker HT User Manual Rev 2 4 Cat no 1506400A 9 Chapter 5 Installation 4 Functional Description The EMS Incubator Shaker HT offers an extended temperature range up to nearly 70 C Uniformity of temperature is also excellent at these elevated temperatures ensuring good assay reproducibility The EMS Incubator Shaker HT uses an individual thermal microplate holder designed for ease of use and uniform heating of each microplate Temperature variation is less than 0 6 C across the microplate To eliminate temperature gradients and edge effects microplates are evenly heated from all sides This ensures assay reproducibility Thermal microplate holders are heated electrically via connectors in the plate carrier An upper heater within the top of the module prevents condensation from forming within the microplate holder For effective mixing a powerful var
13. procedures vary by country WEEE symbol Thermo Fisher Scientific has contracted with one or more recycling disposal companies in each EU Member State European Country and this product should be disposed of or recycled through them Further information on Thermo Fisher Scientific s compliance with these Directives the recyclers in your country and information on Thermo Scientific products which may assist the detection of substances subject to the RoHS Directive are available at www thermo com WEEEROHS Regarding the original packaging and packing materials use the recycling operators known to you For further information contact your local Thermo Fisher Scientific representative Thermo Scientific EMS Incubator Shaker HT User Manual Rev 2 4 Cat no 1506400A 38 Chapter 8 Troubleshooting Guide 8 Troubleshooting Guide A Warning Do not use the instrument if it appears that it does not function properly 8 1 Limitations Control LEDs The orange control LED light emitting diode flashes when a slot is heating up to the programmed target temperature Refer to Section 6 3 for information The LED turns to a continuous orange color when the slot reaches the target temperature However the continuous orange LED may falsely indicate that a slot is at the target temperature under the following conditions e f you remove the thermal microplate holder and then reinsert it into the slot during the initial preheating of the slo
14. reliable daily operation keep the instrument free of dust and spills from liquids It is also advisable to cover the instrument with the dust cover supplied when not in use In the event of any damage contact your local Thermo Fisher Scientific representative for service Abrasive cleaning agents are not recommended because they are likely to damage the paint finish It is recommended you clean the case of the instrument periodically to maintain its good appearance A soft cloth dampened in a warm mild detergent solution will be sufficient Clean the outside of the instrument and the thermal microplate holder with clean low pressure compressed air or a cloth dampened with water or a mild detergent when necessary the cleaning agent as recommended by the manufacturer Do not expose painted surfaces to concentrated acids or alcohols for prolonged periods of time as damage may occur Caution Painted surfaces can be cleaned with most laboratory detergents Dilute Clean the keyboard with a mild laboratory detergent Plastic covers and surfaces can be cleaned with a mild laboratory detergent or alcohol Warning If any surfaces have been contaminated with biohazardous material a mild sterilizing solution should be used See Section 7 4 Periodically inspect the connectors on the thermal microplate holders for dirt or corrosion If dirty or corroded wipe the connectors with a soft cloth slightly dampened with water 7 1 2 Immedi
15. temperature is at the new target setting before you start the processing protocol The instrument does not prevent the processing protocol from starting if the slot is not at the target temperature You can view the actual slot temperatures on the instrument display Refer to Section 6 5 Preheating and heating Module shake speed All slots within the module will shake at the same speed The slots within the module shake at the speed that is programmed into the most recently started processing protocol Incorrect shaking speed may result if you start a protocol within the same module that has a shaking speed different from the other slots already in use To minimize the likelihood of this occurring it is recommended you program all processing protocols within a module with the same shaking speed Thermo Scientific iEMS Incubator Shaker HT User Manual Rev 2 4 Cat no 1506400A 26 Chapter 6 Operation 6 4 3 Operation 6 4 3 1 Starting the operation contact with the temperature controlling connectors at the rear of the Caution If the thermal microplate holder is not latched into position it may lose slot see Section 6 4 1 Placing the microplate Operation Display Press the number of the appropriate slot and then start to start the protocol For example to start slot 1 PLACE 1 Press 1 start The protocol will start and the LED will light up with a red color 09 26 30 The READY state appears while the instru
16. the following markings Type 1415 100 120 Vac 200 240 Vac 50 60 Hz 230 VA max CE mark iEMS Incubator Shaker HT conforms to the following requirements 2006 95 EC Low Voltage Directive 2004 108 EC Electromagnetic Compatibility Directive EMC FCC Part 15 Subpart B Class B 2002 96 EC Waste of Electrical and Electronic Equipment 2006 66 EC Directive on batteries and accumulators and waste batteries and accumulators Safety performance EN 61010 1 1993 A2 1995 IEC 61010 1 1990 A1 1992 A2 1995 including CENELEC Common Modifications US and CA National differences EN 61010 2 010 1994 A1 1996 including CENELEC Common Modifications US and CA National differences EN 61010 1 2001 Ed 2 EMC performance EN 50081 1 1992 Generic emission standard Residential commercial and light industry EN 50082 1 1997 Generic immunity standard Residential commercial and light industry EN 61326 1 1997 A1 1998 Product family standard A2 2001 A3 2003 Test standards Performance limits EN 55022 1998 Class B 150 kHz 1 GHz EN 61000 3 2 1995 A1 1998 Class A A2 1998 A13 1997 A14 2000 EN 61000 3 3 1995 ANSI C63 4 1992 Class B 450 kHz 1 GHz EN 61000 4 2 1995 A1 1998 4 kV CD 8 kV AD Criteria B EN 61000 4 3 1996 A1 1998 3 V m 80 MHz 2 GHz Criteria A EN 61000 4 4 1995 1 kV Criteria B EN
17. the instrument according to the enclosed packing instructions e Use the original packaging to ensure no damage will occur to the instrument during shipping Any damage will incur additional labor charges e Enclose a dated and signed Certificate of Decontamination see Appendix B both inside and attached to the outside of the package in which you return your instrument or other items e Enclose the return authorization number RGA given by your Thermo Fisher Scientific representative e Indicate the fault after you have been in touch with your local Thermo Fisher Scientific representative or the Thermo Fisher Scientific technical service department See Section 9 1 for details on storage and transportation temperatures See also Section 8 3 Service request protocol 7 6 Disposal of the instrument Warning Decontaminate the instrument prior to disposal See Section 7 4 and Appendix B on decontamination Follow laboratory and country specific procedures for biohazardous or radioactive waste dispoal Warning The used batteries are regulated waste and must be disposed of according to local waste regulations The Li batteries have to be changed by an authorized service technician only Instructions for changing the Li batteries are described in the service manual Dispose of the instrument according to the legislation stipulated by the local authorities concerning take back of electronic equipment and waste The proposals for the
18. 3 6 6 4 3 4 Checking the operation stop time Before taking the thermal microplate holder out of the slot you can check the actual time of day when the incubation stopped Press the slot number 1 3 For example Press 1 PLACE 1 INC STOP 09 20 31 Press C to return to the READY state If you take the thermal microplate holder out of the slot you can no longer check the time indicating the stop of the incubation 6 4 3 5 Stopping the operation If you press a slot number and stop before the processing protocol time elapses the protocol stops and incubation stays on The LED will light up with a green color and you will hear a beeping sound For turning off the beeping sound see Section 6 4 3 6 The READY state appears 09 27 30 6 4 3 6 Turning off the audio signal while the instrument is shaking This may damage the shaking Caution Do not place the microplate holder in or remove it from the instrument mechanism If you want to turn off the audio signal but wish to continue the incubation proceed as follows 1 Make sure the control LED is green and that the module is not shaking 2 Remove the thermal microplate holder from the slot The audio signal and the control LED are turned off and the heating of the slot is turned off 3 Re insert the microplate holder into the slot The orange LED is turned on and the heating of the slot turns back on Thermo Scientific iEMS Incubator Shaker HT User Ma
19. 358 9 3291 0415 www thermo com Thermo Scientific EMS Incubator Shaker HT User Manual Rev 2 4 Cat no 1506400A 2 Table of Contents Table of Contents O 0 N OO Bb Y N 2 gt 2 N 0 13 Appendix A Appendix B Appendix C Appendix D Thermo Scientific EMS Incubator Shaker HT User Manual Rev 2 4 Cat no 15064004 Safety Symbols and MarkingS oooonnncccccnonocccononcnccnnnnnncccnnnnnnnnnnnnnnnnnnannnrnrnnnnnrnnnanannrs 7 About the User ET ET ic cie 8 Introduction to the iEMS Incubator Shaker HT rrsnversnvvnnnvvnnnnnvnnnrnnnnnvnnnnnvnnnnnennnn 9 Functional Description rnnnvnrnnnnnnnnnnnnnvnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnen 10 Installation jcccccsicccccsseccceeseccceedeccteecevesencdeesecceetecctecevscdeceeesdcceeevssscdeeveccteecuvshecceuvssceeeyasces 14 Operativo cian ida o a Ain 19 MAINTENANCE ic 34 Troubleshooting Guide comicios iii dina 39 Technical Specifications rrrrsnnvnrnnnnnvrvnnnnnvrnnnnnnvnnnennnnvnnennnnvnnennnnvnnennnnennennnnennennnnnesne 44 Ordering Information sasira diiniita rinda 48 Warranty Certificate iii ii A es 49 AAA meA eAP A 50 Glossary and Abbreviations iio ini 53 Thermo Scientific EMS Incubator Shaker HT Quick Reference Guide 54 Certificate of Decontamination evvnvrnvnnnnnnnnnnennvnnnnnnnnnennvnnnnnnnnnnnnennnnnnnnnnnnnnnnnnnnnnununene 55 Thermo Scientific EMS Incubator
20. 61000 4 5 1995 2 kV line to ground 1 kV line to line Criteria B EN 61000 4 6 1996 3 Vims 150 kHz 80 MHz Criteria A EN 61000 4 8 1994 3 A m Criteria A EN 61000 4 11 1994 30 10 ms Criteria B 60 100 ms Criteria C 100 20 ms Criteria B gt 95 5 s Criteria C Thermo Scientific EMS Incubator Shaker HT User Manual Rev 2 4 Cat no 1506400A 47 Chapter 10 Ordering Information 10 Ordering Information Contact your local Thermo Fisher Scientific representative for ordering and service information Code 5112250 Instrument EMS Incubator Shaker HT with three 3 thermal microplate holders HT 220 240 V 5112257 EMS Incubator Shaker HT with three 3 thermal microplate holders HT 100 120 V 5921210 EMS thermal microplate holder HT 10 1 List of accessories and recommended spare parts Code Item 15064004 EMS Incubator Shaker HT User Manual 1610400 Dust cover for EMS Incubator Shaker 1 module 5180190 RS Device link cable 1210940 1210550 Fuse 3 A slow Power cord Euro 230 V 1210520 Power cord USA 100 V and 115 V 5921210 EMS thermal microplate holder HT Thermo Scientific EMS Incubator Shaker HT User Manual Rev 2 4 Cat no 1506400A 48 Chapter 11 Warranty Certificate 11 Warranty Certificate Thermo Fisher Scientific Microplate Instrumentation Business products are fully guaranteed against def
21. ashing and potential cross contamination from well to well do not overfill wells for a given shaking speed setting Caution Splashing may occur if you use shaking with wells that contain too When the well is filled to 50 of its nominal volume the shake speed setting should not exceed value 4 It is recommended to use the setting 3 in most cases Operation Display You can activate shaking without heating as a separate function by pressing the shake key Press shake PLACE NUMBER Press the slot number 1 3 For example Press 1 PLAGET 900 rpm 00 00 20 To start shaking press start The shaking time and speed are the same as the parameters set in the programming phase After pressing shake you can select the slot numbers by pressing number keys or by scrolling with the J Enter key Within a module if a slot has a protocol in progress with a shaking speed of 0 PLACE 2 another protocol with a nonzero shaking PROHIBITED OPERATION speed will not start and the display will show After the shaking protocol has finished 09 29 35 the control LED turns to green and you will hear a beeping sound To stop shaking before the shaking time has elapsed Press the slot number and the stop key The protocol stops the LED turns to green and you will hear a beeping sound Thermo Scientific EMS Incubator Shaker HT User Manual Rev 2 4 Cat no 15064004 32 Chapter 6 Operation Operation Display
22. ate Wipe up all spills of liquids immediately from all surfaces of the instrument to prevent leakage into the electronics and mechanics of the instrument Clean and decontaminate the surfaces if necessary Although the iEMS Incubator Shaker HT is constructed from high quality materials you must immediately wipe away spilled saline solutions solvents acids or alkaline solutions from outer surfaces to prevent damage and wipe with deionized distilled water Thermo Scientific iEMS Incubator Shaker HT User Manual Rev 2 4 Cat no 1506400A 34 Chapter 7 Maintenance 7 2 How to replace fuses The fuses are located on the left side of the instrument Fig 4 1 item 8 1 Switch the instrument OFF Unplug the instrument and disconnect the power cable from the power input socket 2 Use a small flat screwdriver to pry the voltage selector fuse block assembly away from the power entry port housing and remove Fig 7 1 g m yes Fig 7 1 Opening the voltage selector fuse block assembly 3 Replace the blown fuses with the same type 3 0 A See Fig 7 2 1 Fuses 2 Voltage selector Nee A y 7 Fig 7 2 Fuse block assembly 4 Place the fuse holder back into the slot and push it in until you hear a click 5 Connect the power cable to the instrument and switch it ON Thermo Scientific EMS Incubator Shaker HT User Manual Rev 2 4 Cat no 1506400A 35 Chapter 7 Maintenance 7 3 Disposal of materials Re
23. bly Thermo Scientific EMS Incubator Shaker HT User Manual Rev 2 4 Cat no 1506400A 16 Chapter 5 Installation 3 Remove the voltage selector module Fig 5 2 item 4 rotate it 180 and replace Make sure the correct voltage value is visible through the slit at the end of the voltage selector fuse block assembly Fig 5 2 item 3 230 V Fuse Fuse holder Slit showing the correct voltage Voltage selector module PONS Fig 5 2 Selecting the correct voltage 4 Slide the fuse block assembly back into the power entry port housing Snap the assembly securely into place 5 Reinsert the power cable 5 3 2 Mains supply cable connection Never use a mains supply cable other than the Thermo Warning Never operate your instrument from a power outlet that has no ground Scientific mains supply cable designed for your region 1 Ensure the mains switch Fig 4 1 item 7 at the back of the left side panel is in the OFF position 2 Connect the mains supply cable to the mains input socket Fig 4 1 item 6 at the back of the left side panel 3 Connect the instrument to a correctly installed line power outlet that has a protective conductor that is grounded Thermo Scientific iEMS Incubator Shaker HT User Manual Rev 2 4 Cat no 1506400A 17 Chapter 5 Installation 5 3 3 Setup Power on the instrument The firmware version is shown on the display Set the clock time 1 While in the READY state press C
24. cal service department The Warranty Claim Technical Sheet contains a more detailed description of the fault symptom or condition Give all the necessary information to the distributor who will fill out and forward the Warranty Claim Technical Sheet to the Thermo Fisher Scientific technical service department Check Section 7 5 How to pack for service You will find instructions on how to proceed before shipping the instrument for service Check that any necessary decontamination procedure has been carried out before packing See Section 7 4 Decontamination procedure Ensure the Certificate of Decontamination see Appendix A as well as the return authorization number RGA are sent with the instrument The Thermo Fisher Scientific technical service department will keep you up to date with the progress of service and provide you with any further details you might need for example on maintenance serviceability troubleshooting and replacement Thermo Scientific EMS Incubator Shaker HT User Manual Rev 2 4 Cat no 1506400A 43 Chapter 9 Technical specifications 9 Technical Specifications 9 1 General specifications General specifications Overall dimensions 400 mm W x 450 mm D x 200 mm H 15 7 in W x 17 7 in D x 7 8 in H Weight total 15 kg 33 lbs Mains power supply 100 120 Vac 50 60 Hz nominal 200 240 Vac 50 60 Hz nominal Power consumption 230 VA max Heat di
25. cific application Other markings used in the documentation o Note Marks a tip important information that is useful in the optimum I operation of the system or an item of interest Thermo Scientific EMS Incubator Shaker HT User Manual Rev 2 4 Cat no 1506400A 7 Chapter 2 About the User Manual 2 About the User Manual The user manual has been written for the actual end user for example laboratory technician and provides information on the Thermo Scientific iEMS Incubator Shaker HT including installation and operating instructions Read the manual in its entirety prior to operating the instrument The user manual has been designed to give you the information you need to e Review safety precautions e Install the EMS Incubator Shaker HT e Carry out incubating and shaking procedures e Perform basic maintenance procedures e Troubleshoot the instrument performance e Maintain the instrument The user manual also describes features and specifications of the EMS Incubator Shaker HT hardware and onboard software Chapter 6 Operation explains the operating procedures The user should be familiar with the contents of Chapter 7 on maintenance For warranty and ordering information refer to Chapters 10 Ordering Information and 11 Warranty Certificate In an effort to produce useful and appropriate documentation we appreciate your comments on this document to your local Thermo Fisher Scientific representative Th
26. cimil aida i 22 SP A A A AA A A A NES 34 40 SPl shing O 22 32 40 T Temperature settingi a O ere 26 41 Thermal microplate holder viii a da 13 clean uret 34 placing iia aia aaa 24 Transport d magexaas di GEERT 15 Transport package ii Re SENT 14 15 U Unpaid fs ene 14 V VIEWING parameter unica A ea one eee ia 33 40 Voltage selettor cd Te Te SSE 16 W War Y LA daud delete damene Mnd AA AD et 14 49 Wed A a 15 44 Working Ar auu guan Aadne eee ieee tale 15 Thermo Scientific iEMS Incubator Shaker HT User Manual Rev 2 4 Cat no 1506400A 52 Chapter 13 Glossary and Abbreviations 13 Glossary and Abbreviations ElA Enzyme immunoassay EN European Norm EU European Union IEC International Electrotechnical Commission Incubator An apparatus for maintaining optimal conditions temperature humidity etc for growth and development Microplate A rigid or framed polystyrene plate with microwells in different well formats for example 6 12 24 48 96 384 864 etc wells for ease of use in performing multiple tests through techniques such as EIA or ELISA Orbital shaking The shaking of a microplate in a circular movement with a fixed orbit and adjustable speed rpm Revolutions per minute 13 1 Keywords for web pages drug discovery ElA ELISA enzyme immunoassay immunoassay incubating incubator incubator shaker liquid handling microplate microplate incubator Microtit
27. contamination will incur additional labor charges or at worst the items will be returned for proper cleaning Before returning any instrument s or item s ensure they are fully decontaminated Confirm A or B status Name Address Tel Fax Name Serial no A I confirm that the returned items have not been contaminated by body fluids toxic carcinogenic or radioactive materials or any other hazardous materials B I confirm that the returned items have been decontaminated and can be handled without exposing the personnel to health hazards Materials used in the unit Chemicals Biological e Radioactive Specific information about contaminants Decontamination procedure Date and place Signature Name block capitals The signature of a Radiation Safety Officer is also required when the unit has been used with radioactive materials This unit is certified by the undersigned to be free of radioactive contamination Date and place Signature Name block capitals PHOTOCOPIABLE 1 Please include decontaminating solution used Thermo Scientific EMS Incubator Shaker HT User Manual Rev 2 4 Cat no 1506400A 55 Appendix C Thermo Scientific EMS Incubator Shaker HT Feedback Form Please send to Thermo Fisher Scientific Oy Fax 358 9 32910415 Appendix C Thermo Scientific EMS Incubator Shaker HT Feedback Form Cat no Se
28. ctual TRUE slot temperature and o heating status HEAT OFF or HEAT ON PLACE SET ud are displayed HEAT ON TRUE 37 0 C If the thermal microplate holder is not installed or inserted correctly the PLACE 1 SET 30 0 C display will show HEAT ON TRUE C Press start to turn the heating on Press stop to turn the heating off Press a number key or 4 Enter to move to another slot Before the temperature has reached the set value the LED flashes with an orange color After the temperature has reached the set value the orange LED lights up continuously Press temp 0 to display the heating status of all slots If the heating is ON in every slot the display will show PLACES 1 3 HEAT ON If the heating is OFF in every slot the display will show PLACES 1 3 HEAT OFF If some slots are ON and some are OFF the display will show PLACES 1 3 HEAT ON OFF At this point press start to turn the heating ON in all slots or press stop to turn the heating OFF in all slots Press C to return to the READY state READY 09 29 10 Warning Microplate holders will be hot when touched after use Thermo Scientific EMS Incubator Shaker HT User Manual Rev 2 4 Cat no 1506400A 31 Chapter 6 Operation 6 6 Shaking while the instrument is shaking This may damage the shaking Caution Do not place the microplate holder in or remove it from the instrument mechanism much liquid for a given speed To avoid spl
29. d into its normal operating position Ensure you have inserted the thermal microplate holder correctly into the slot before you start the processing protocol When inserting a microplate holder into a slot push the holder completely in and down to latch it into its normal operating position see Fig 6 3 and Fig 6 4 If the microplate holder is not latched into position it may lose contact with the temperature controlling connectors at the rear of the slot while the instrument is shaking This may damage the shaking Caution Do not place the microplate holder in or remove it from the instrument mechanism Incubator slot preheating When you power on the instrument heating of the incubator slots does not occur automatically You must turn the temperature function on see Section 6 5 Preheating and heating after you have powered on the instrument Allow the slots to reach the programmed target temperature before you start any processing protocol The slot has reached the target temperature when the orange LED has stopped flashing and stays continuously orange Before you start the processing protocol ensure the temperature of the slot is within the acceptable range appropriate for the application to be carried out You can view the actual slot temperature on the instrument display see Section 6 5 Preheating and heating Changing programmed temperature setting When you change the programmed temperature setting ensure the slot
30. d without shaking lt 0 6 C across the entire plate calculated as the difference between the maximum and minimum well temperatures of wells A1 A12 H1 H12 and E7 after 1 hour at 65 C with an ambient temperature of 24 C with 200 ul water well with a plate sealer and without shaking Evaporation lt 300 mg plate after 1 hour at 37 C without plate sealer 9 3 Shaker specifications Performance of the shaker with flat bottomed microplates with nominal voltages Frequency 400 1400 revolutions per minute rpm in steps of 250 rpm Speed settings 0 0 rpm 1 400 rpm 2 650 rpm 3 900 rpm 4 1150 rpm 5 1400 rpm Tolerance 50 rpm Diameter 1 mm radius 0 5 mm Programmable shaking time Up to 48 hours in steps of 1 second Programmable interval time Up to 48 hours in steps of 1 second Thermo Scientific EMS Incubator Shaker HT User Manual Rev 2 4 Cat no 1506400A 45 Chapter 10 Device Interfaces 9 4 Safety specifications EMS Incubator Shaker HT fulfills the following requirements EN 61010 1 1993 A2 1995 IEC 61010 1 1990 A1 1992 A2 1995 including CENELEC Common Modifications US and CA National differences EN 61010 2 010 1994 A1 1996 including CENELEC Common Modifications US and CA National differences EN 61010 1 2001 Ed 2 The safety specifications are also met under the following environmental conditions in addition to or i
31. decontaminants e Ethanol 70 e Virkon solution 1 3 e Glutaraldehyde solution 4 e Chloramine T e Microcide SQ 1 64 Caution If local or laboratory regulations prescribe regular decontamination it is not advisable to use formaldehyde since even small traces of formaldehyde affect the enzyme being used in ElA tests in a negative way resulting in bad test results Warning Always use disposable gloves and protective clothing and operate in a well ventilated area Thermo Scientific EMS Incubator Shaker HT User Manual Rev 2 4 Cat no 1506400A 36 Chapter 7 Maintenance 7 4 1 How to decontaminate the instrument surface In the event of spills of infectious agents on the surfaces of the instrument perform the following decontamination procedure 1 Switch the power off and unplug the instrument 2 Use disposable gloves 3 Absorb the spill with disposable towels 4 Dampen a disposable towel with 2 glutaraldehyde containing decontaminating solution and clean the surfaces of the instrument 5 Place the disposable towels into a biohazard container 7 4 2 How to decontaminate the thermal microplate holder In the event of spills of infectious agents on the surfaces of the thermal microplate holder perform the following decontamination procedure Warning Microplate holders will be hot when touched after use Allow the holder to cool down prior to performing the decontamination procedure 1 Use disposable gl
32. discrepancy report e Manufacturing Quality Report e EMS Incubator Shaker HT User Manual Caution Do not touch or loosen any screws or parts other than those specially designated in the instructions Doing so might cause misalignment and will invalidate the instrument warranty To lift the instrument put your fingers under the bottom on both sides and lift it with your back straight Caution When unpacking the instrument it is recommended two people lift the instrument together taking proper precautions to avoid injury Retain the original packaging and packing material for future transportation The packaging is designed to assure safe transport and minimize transit damage Use of alternative packaging materials may invalidate the warranty Also retain all instrument related documentation provided by the manufacturer for future use Thermo Scientific EMS Incubator Shaker HT User Manual Rev 2 4 Cat no 1506400A 14 Chapter 5 Installation 5 2 2 Checking delivery for completeness Check the enclosed packing list against order In case of any deviations contact your local Thermo Fisher Scientific representative 5 2 3 Checking for damage during transport Visually inspect the transport package the instrument and the accessories for any possible transport damage If the carton has been damaged in transit it is particularly important that you retain it for inspection by the carrier in case there has also been damage to th
33. e instrument Neither the manufacturer nor its agents can be held responsible for any damage incurred in transit but the manufacturer will make every effort to help obtain restitution from the carrier Upon receipt of the carrier s inspection report arrangements will be made for repair or replacement Visually check all interconnections in the basic instrument Check that there are no loose parts inside the instrument If any parts are damaged contact your local Thermo Fisher Scientific representative 5 2 4 Climatic requirements When you set up your EMS Incubator Shaker HT avoid sites of operation with excess dust vibrations strong magnetic fields direct sunlight draft excessive moisture or large temperature fluctuations e Make sure the working area is flat dry clean and vibration proof and leave additional room for accessories cables reagent bottles etc e Leave sufficient space at least 10 cm on both sides and at the back of the unit to allow adequate air circulation e Make sure the ambient air is clean and free of corrosive vapors smoke and dust e Make sure the ambient temperature range is between 10 C 50 F and 40 C 104 F e Make sure relative humidity is between 10 and 90 non condensing The iEMS Incubator Shaker HT does not produce operating noise at a level that would be harmful No sound level measurements are required after installation Warning Do not operate the instrument in an e
34. ective parts and materials including defects caused by poor workmanship for a period of twelve 12 months from the date of delivery Thermo Fisher Scientific will repair or replace defective parts or materials during the term of warranty at no extra charge for materials and labor provided that the products were used and maintained in accordance with Thermo Fisher Scientific s instructions The warranty is invalid if products have been misused or abused For this warranty to be effective the product must have been purchased either directly from Thermo Fisher Scientific or from an authorized Thermo Fisher Scientific distributor The guarantee is not transferable to a third party without prior written approval from Thermo Fisher Scientific This guarantee is subject to the following exclusions e Any defects caused by normal wear and tear e Defects caused by fire lightning flood earthquake explosion sabotage war riot or any other occurrence of the character listed above e Refurbished products that are subject to different warranty conditions THIS WARRANTY IS IN LIEU OF ALL OTHER EXPRESSED OR IMPLIED WARRANTIES INCLUDING BUT NOT LIMITED TO ANY IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE The seller is not liable for any loss or damage arising out of or in connection with the use of the product or other indirect damages Full warranty terms and conditions can be obtained from your local Thermo Fisher Sc
35. ent while the instrument is shaking This may damage the shaking mechanism Fig 6 2 Microplate in the thermal microplate holder Be careful not to spill the contents of the microplate when you load it into the thermal microplate holder and when you insert the holder into the slot Carefully insert the plate into the microplate holder and close the cover Caution The covers of the thermal microplate holders are not spring loaded but you should take care not to trap your fingers when closing the cover of the holder Avoid bumping the microplate holder while inserting it into the slot Thermo Scientific EMS Incubator Shaker HT User Manual Rev 2 4 Cat no 1506400A 24 Chapter 6 Operation When you are inserting a thermal microplate holder into a slot push the holder completely in Fig 6 3 and then down Fig 6 4 to latch it into its normal operating position Caution Do not place the microplate holder in or remove it from the instrument while the instrument is shaking This may damage the shaking mechanism Fig 6 4 Pushing down the microplate holder to latch it properly o Note If the thermal microplate holder is not latched into position it may lose I contact with the temperature controlling connectors at the rear of the slot Thermo Scientific EMS Incubator Shaker HT User Manual Rev 2 4 Cat no 1506400A 25 Chapter 6 Operation 6 4 2 Before pressing start Ensure the thermal microplate holder is latche
36. er plate microwell plate orbital shaker orbital shaking orbital shaking incubator plate separation technique s shaker shaking solid phase assay s Thermo Fisher Scientific Thermo Scientific Thermo Scientific EMS Incubator Shaker HT User Manual Rev 2 4 Cat no 1506400A 53 Appendix A Thermo Scientific EMS Incubator Shaker HT Quick Reference Guide Appendix A Thermo Scientific EMS Incubator Shaker HT Quick Reference Guide 1 Place the microplate into the thermal microplate holder so that well A1 is located in the upper left corner when viewed with the numbers on the holder s handle the right side up Refer to Fig 6 2 2 Insert the thermal microplate holder into an incubation slot Refer to Fig 6 3 and Fig 6 4 Switch the instrument ON Refer to Fig 4 1 item 7 4 Program the incubation and shaking parameters for the appropriate slot Press the slot number 1 3 and Enter For all slots press 0 J Enter the incubation and shaking parameters in the following order refer to Section 6 2 and confirm each selection by pressing 4 Temperature C Incubation time hh mm ss Shaking time hh mm ss Interval time hh mm ss Shaking speed as shown below Value Shake speed rpm 0 400 650 900 1150 1400 5 Press temp followed by the slot number to start the preheating of the thermal microplate holder Refer to Section 6 5 6 Wait until the control LED changes f
37. ermo Scientific EMS Incubator Shaker HT User Manual Rev 2 4 Cat no 1506400A 8 Chapter 3 Introduction to the EMS Incubator Shaker HT 3 Introduction to the iEMS Incubator Shaker HT The iEMS Incubator Shaker HT is available as a one cabinet model allowing the capacity of three 3 microplates 3 1 Intended use The iEMS Incubator Shaker HT is a high quality standalone microplate incubator and orbital shaker intended for laboratory research use by professional personnel The instrument is used to incubate and shake microplates that meet the SBS standards with a temperature of up to 69 C and a shaking speed of up to 1400 rpm For verification of the entire system it is recommended Good Laboratory Practices GLP be followed to guarantee reliable analyses Use for self testing is excluded 3 2 Principle of operation The iEMS Incubator Shaker HT consists of a keyboard and one incubation module with three 3 incubation slots All slots can be used for processing microplates individually within certain limitations A control LED above each slot indicates the status of that slot The keyboard is used to enter processing parameters and to start thus programmed protocols These protocols can be the same for all slots or entered individually for each slot The parameters are stored in the instrument memory until new parameters are entered lt is possible to change the parameters of one slot without affecting the parameters
38. fer to local regulations for the disposal of infectious material Warning The samples can be potentially infectious Dispose of all used microplates disposable gloves syringes disposable tips etc as biohazardous waste 7 4 Decontamination procedure A A A A Caution Only use a cloth dampened with a decontaminant to decontaminate the instrument The use of spraying may damage the instrument Warning To prevent electric shock do not reach into a slot and or touch the power contacts at the rear of the slot when the power is turned on to the instrument If you need to access the slot for example for cleaning turn the power off to the instrument and unplug the power cable Decontamination should be performed in accordance with normal laboratory procedures Any decontamination instructions provided with the reagents used should be followed A decontamination procedure is only recommendable when infectious substances have been in direct contact with any part s of the instrument If there is any risk of contamination with biohazardous material the procedure recommended below or some other corresponding decontamination procedure must be performed It is strongly recommended the complete decontamination procedure is performed before relocating the instrument from one laboratory to another Refer to Section 7 5 How to pack for service Decontamination is not required for the proper functioning of the instrument Example of
39. ia radios 27 28 preheating nd heating iii A ad 31 A oo 9 19 programming Protocols vemoss ai aladdin ddrean i 20 Shakira dd dodo road EOS EROS 32 Ordering information ie 48 P Packing disposal Of Materia Sucina aaa 38 TORSORVIGG ii A RA A AA AAA a AAA AAA da 38 A a E 15 Parameters INGUDATIOMLEMPSAtUNe acta 20 incubation timeni e a ai Eee ii a 21 interval dit A A eide aea aa ae eile 21 same for al slots ii na 20 SOM ita A A da 20 Shaking ME asi rias 21 VICWING RS 33 PAUSINI ee 33 Thermo Scientific EMS Incubator Shaker HT User Manual Rev 2 4 Cat no 15064004 51 Chapter 12 Index Placing the microplate into thermal microplate holder rrrrnrvvrrnnnennnonnnnvevnnnnnnanennnarnarnnnnentnnnnnnrnnnen 24 ld a a EE 9 Power Calle its usar ta are Ad 17 A NN 26 31 PODIAMOS A TE dnne 20 P OO OS a la dio SERT 20 Q QUICK Reference Guide ii AET REA REEE des fens Nav ana REEE des AARNA Er AA nas Neda PENTE 54 R ETE E EA E AAE E A E AA Aar AA E A AET AA N 30 42 S Senest 34 43 EA O Ang 38 reduest Protocol Eee 43 Setting parameter E A EEE da 20 NS 18 SAI A tod 32 PAU AA 33 SPECON men a A E ia 26 39 41 UV ui A AA A AEAT 21 Ste o operation at A a eceid baa cee nk 15 SJ t Status RN NON 23 Slottemperatlr aei e E S ad 41 Slot Ww rmup TIME cimas di E E E RE E E added E 41 ee ONE E EIn LEE E A A da 44 generale cie a a ia as 44 INCUDALO uadressert 45 0 EE ME MH O AE a E OE 46 SNA A a NT 45 Speed settinOS
40. iable speed orbital shaker is incorporated Featuring an orbit of 1 mm and speeds from 400 to 1400 rpm in 250 rpm increments the shaker motion ensures efficient mixing of even very viscous liquids All slot positions within a module are shaken simultaneously Each slot of the iEMS Incubator Shaker has a control LED light emitting diode that displays the status of the slot The LED will change to a specific color based on the status of the slot When the incubation and shaking operations end the instrument also gives an audio signal 4 1 iEMS Incubator Shaker HT front view TA Display Keyboard Control LED light emitting diode Incubation slot Incubation module with three 3 slots Mains input socket Mains switch Voltage selector and fuses Fig 4 1 EMS Incubator Shaker HT front view ON OOP WN Thermo Scientific iEMS Incubator Shaker HT User Manual Rev 2 4 Cat no 1506400A 10 Chapter 5 Installation 4 2 EMS Incubator Shaker HT rear view Cooling air inlet DIP switches not in use Device link RS 485 not in use Computer RS 232C device link RS 485 not in use Cooling air outlet Type label OU PWN gt Fig 4 2 EMS Incubator Shaker HT rear view Thermo Scientific EMS Incubator Shaker HT User Manual Rev 2 4 Cat no 15064004 11 Chapter 5 Installation 4 3 Keyboard and display 7 8 9 stop 4 5 6 temp 4 2 3 shake OC st
41. ie a A AA A EPen 56 FUSE block assembly iii cata Da Aaa AA E aa aa O Riad denies 16 FSS 35 44 H FHS ATM A ere A ATTAT 31 Heating time iii e a ia 21 A cake Ads aE a a Ea eaaa EE A E 13 I INCUBATION tempera aiii a ia AA ali aa 20 Incubation MES macs ira leds alicia idad 21 INCUBATION time TACK Y a A aan dqkiuvitsteu 40 Incubator slot preheating eiii dde 26 39 Inserting THE Microplate viii e ia 24 Thermo Scientific EMS Incubator Shaker HT User Manual Rev 2 4 Cat no 1506400A 50 Chapter 12 Index Installation ARRET 14 CAMION ae aaa ihe etal eee 46 CMO CKISU cele dee ete A daa 14 AU A ii Ao 16 Mi Aer 15 listrument au22rr Aine Gis nie ines aie uke ete 9 AVANT EE a 9 CIEANING A A A ay de a A E 34 CE CO MTANUINAtlON A ii 36 GIS POS iii A A A da 38 INSpectinguua casa A id 15 Marking Smari e a AA AA A are 47 PACKING Tor Sory CE aea NN 38 pa kih O ca A A a eae a 14 l tended use ici AT T bavi A A A E ATT 9 A ets ct ne ne ee E A T 21 K KO YO ard O 12 CLEANING 20s ai hk A een dain ata al wa ie adn ie 34 Corbera 12 L EC paint A T EO EIA E A anata ata dedi aint 12 M Mains supply cable monica At a 17 Mains SWItCh2 0d A te ene ila Mee eel 17 Maintenance mitico ta dogg oie es een dn edie laa nights 34 Microplate disposalofuivnv rundgang hii OE EE E EE A E 36 42 DIACING onien O 24 Microplate h lder iii e a iris 13 O Operating conditlonsu miii ii a aii ai 15 Operation NoORMalLasni dt ra AS PAE E EE t
42. ientific dealer 11 1 Warranty limitations e Consumables are not included in the warranty Thermo Scientific iEMS Incubator Shaker HT User Manual Rev 2 4 Cat no 1506400A 49 Chapter 12 Index A ACCESO a a 48 INS POCO isso EEE EE EE EE EE ee 15 UNPACKING eu ra a A A A Gen bastante 14 O A a ke pa O AN 40 Application A da 56 AUdiO SIA siirad a abia 23 turning Oh a a T E E E S daa coa 28 C C talog numbers vsciioara aii A E E E 2 Certificate of Decontaminationi uauanarvkinagdmbu A A a 55 CHECK IS SEE EE EE EP EEE PE 14 Checking parameters iii da 33 Clock TING EEE NT 18 Compl int Order a A cia caia 43 Consumables warranty MINS iii A a ie 49 Contact information adi ad 57 Control LEDS cion ii Seta a Wired deeds A A E aaa Masa A es 10 23 39 D DECONTAMINATION siii ad 36 38 A hjul 55 Oda cau aavededaig 37 o NN 36 thermal microplate NOIED ccc cece et enne ee ee eee eee ee cea e ae eee ea eae ee eee eae aceasta aaa ease aeaeaees 37 DIMENSIONS aria daa 44 Directive S 0 a a illa 47 Disibfectais anio anita EE Ee A A a aiii 36 DIS a ease SS 12 Disposal IVSEPUIMG IE seirinin ove chee ceecanteecedendececechevededexaeerecesteecedexdecneutunsdeescestendsesudeucselbeweadentrecedartieeedsy 38 Maternal EEE e NN iaar iia 36 E Environmental CONdItiONS ccccccccccccncncnnnonononononononononononnnn nono nono non nro nn nn nn nero nn nn rn nro none nn nn nn rn rn rn r nn rn nnnnnnnna 44 Evaporati AO 40 45 F Fecdback Fo
43. inocinicccccicccno 25 Removing the microplate holder lifting the handle oooooococccococacanananananonononononos 30 Removing the microplate holder pulling the holder out rrrrrrrrrrnssnnnrrrrrrrnnnn 30 Opening the voltage selector fuse block assembly mrrrrrrrrrrrrrrrnnnrrr ar rrrrerrnnr ner 35 FUSE DIOCKGSSCIMIDIV i sk anke st Ran bordene AE dabas tan 35 Installation CHeCk IST nasa ee Kuan Be ae te deeds 14 Speed setings OF THE Shaker 0 Mame A tdi 22 LED COlIOF COGCS trap A bn BE een 23 Thermo Scientific iEMS Incubator Shaker HT User Manual Rev 2 4 Cat no 1506400A 6 Chapter 1 Safety Symbols and Markings 1 Safety Symbols and Markings These symbols are intended to draw your attention to particularly important information and alert you to the presence of hazards as indicated Safety symbols and markings used on the EMS Incubator Shaker HT Power ON O Power OFF SN Serial number REF Catalog number Date of manufacture Consult Instructions for Use WEEE symbol This product is required to comply with the European Union s Waste Electrical amp Electronic Equipment WEEE Directive 2002 96 EC M lg B Warning markings used in the documentation IN Warning Risk of electric shock A Warning Biohazard risk A Warning Hot surface risk of burns A Warning Risk of injury to the user s Caution Risk of damage to the instrument other equipment or loss of performance or function in a spe
44. ion 6 2 Programming protocols Use the keyboard to enter processing parameters and to start thus programmed protocols You can program a single protocol for all slots or an individual protocol for each slot The parameters are stored in the instrument memory until you enter new parameters You can change the parameters of one slot without affecting the parameters of the other slots In one module all slots have the same incubation temperature and they shake at the same speed You should preheat the slots to the programmed target temperature before you start any protocol see Section 6 5 Preheating and heating Operation Display Switch the power ON 09 25 15 Note If the power is already on any shaking or heating must be turned off in the slot to be able to program a protocol To program the incubation and shaking parameters press the appropriate slot number 1 3 For example PLACE 1 09 25 15 Press 1 Enter TEMPERATURE 37 0 C If you want to program the same parameters to all slots ALL PLACES Press 0 J TEMPERATURE 37 0 C Program the new temperature value by dialing the number keys or press 4 Enter to accept the existing one Note Check where the cursor underline is located before you enter a value The value will be entered at the location of the cursor For example Press 50 1 TEMPERATURE 50 0 C INC TIME 00 00 00 Note If you want to interrupt the programming press Stop to ret
45. ment is shaking This may damage the shaking Caution Do not place the microplate holder in or remove it from the instrument mechanism 6 4 3 2 Checking the operation time During operation you can check the remaining incubation time by pressing the slot number For example Press 1 If the remaining time is 23 minutes and PLACE 1 15 seconds the display will show TIME LEFT 00 23 15 Press C to return to the READY state 6 4 3 3 Starting a program during operation in another slot Within a module if a slot has a protocol in progress with a shaking speed of 0 another protocol with PLACE 2 shaking will not start and the display will show PROHIBITED OPERATION o Note The slots within a module shake at the speed that is programmed into the most recently started processing protocol For example if there is a protocol in progress with speed 5 and the adjoining slot is started with speed 3 all slots within the module will shake at speed 3 Thermo Scientific EMS Incubator Shaker HT User Manual Rev 2 4 Cat no 15064004 27 Chapter 6 Operation Within a module a second protocol with a programmed temperature different from the protocol already in PLACE 3 progress will not start and the display PROHIBITED OPERATION will show When the protocol is completed the LED will light up with a green color and you will hear a beeping sound The heating stays on For turning off the beeping sound see Section 6 4
46. n excess of those stated in the operating conditions Altitude up to 2000 m Temperature 5 C 40 C Mains supply fluctuations 10 from nominal Installation category overvoltage category ll according to IEC 60664 1 see Note 1 Pollution degree 2 according to IEC 60664 1 see Note 2 e Note 1 o Note 2 The installation category overvoltage category defines the level of transient overvoltage which the instrument is designed to withstand safely lt depends on the nature of the electricity supply and its means of overvoltage protection For example in CAT II which is the category used for instruments in installations supplied from a supply comparable to public mains such as hospital and research laboratories and most industrial laboratories the expected transient overvoltage is 2500 V for a 230 V supply and 1500 V for a 120 V supply The pollution degree describes the amount of conductive pollution present in the operating environment Pollution degree 2 assumes that normally only nonconductive pollution such as dust occurs with the exception of occasional conductivity caused by condensation Both of these affect the dimensioning of the electrical insulation within the instrument Thermo Scientific EMS Incubator Shaker HT User Manual Rev 2 4 Cat no 1506400A 46 9 5 In conformity with the requirements Chapter 10 Device Interfaces EMS Incubator Shaker HT bears
47. ng the handle a Fig 6 6 Removing the microplate holder pulling the holder out 3 Place the microplate holder on a heat resistant surface Open the cover and remove the microplate temperatures reach 80 C 5 C in the unlikely event that temperature control to a holder is lost or enters a runaway condition Microplate holders at this temperature will be hot when touched To help prevent loss of temperature control ensure the power contacts on the back of the microplate holder are clean and free from dirt or corrosion and that the holder is correctly inserted into the slot before you start the processing protocol A Warning The thermal microplate holders are designed to stop heating if Instrument reset due to power loss If the power to the instrument is lost the instrument will reset to the READY mode when power is restored All shaking and incubation functions including timing will be turned off No LEDs will be lit and the current time of day will be shown on the display Thermo Scientific EMS Incubator Shaker HT User Manual Rev 2 4 Cat no 1506400A 30 Chapter 6 Operation 6 5 Preheating and heating AN Operation Display Press temp followed by the slot number to display the temperature in a particular slot or to start the preheating of the thermal microplate holders PLACE NUMBER Press temp Press the slot number 1 3 For example Press 1 PLACE 1 SET 30 0 C HEAT OFF TRUE 25 7 The a
48. nnnnnnvvnnnnnvnvnnnnnnnvnnnnnnnvnnennnnvnnennnnnennennnnvnnennnnennennnnennernnnenne 39 LIMITATIONS Sect rona Silos eh td de dae te 39 Warnings nd Caution tara eneeier 39 Installation Men 39 Generala aa ii a Ad dise 40 Temperaturer ci an AEE En AEE 41 ShakiNgis iia add tada 41 OPEN A a a 42 MalNten aC ito a IA Ponape in Gan eek 42 Service request protocol ico cidad idad diia daci n dd dd dad 43 Technical Specifications rrrrnnronnnnnnvrnnnnnnvrnnnnnnvnnnnnnnnvnnnnnnnvnnensnnvnnennnnvnnennnnnnrnsnnnnesne 44 General specifications secme iaria reunaa rra a rr eein erkan erdirir 44 Incubator SPECIFICATIONS merisead aa rra rra 45 Shaker SpecificatiONS serres erp anane eaea enu n nn E KAEKA EERE PARRAS NE KEEA N KEETE rr naar ra nenas 45 Safety Specification e da A OEL EAE 46 In conformity with the requirementS nnocccnnnicccnnonocnnnnonannnnnnnonnn nn nr nnnn nn anna nn rr rrnnn rra 47 Ordering Information rerrrnnvnnnnnnnvnrnnnnnvnvnnvnnvnvensnnvnvensnnnnnnensnnvnnensnnnnnersnnnnnessnnnnnennnen 48 List of accessories and recommended spare partS srrrrransnnvnnannnnrnnrnnnnrnerrennnrnesrennnne 48 Warranty Certificate cionado ia ia ida as 49 Warranty llMitations isla credos eli adi 49 O APP NU OOC AQ PP E E doeewbdaveusele ene 50 Glossary and Abbreviations rerrnnvvvnnnnnvnvnnnnnvnvnnnnnnvnvnnrnnnnvnnennnneensnnnnenensnnnneensnnnnsenennnn 53 Keywords for web pages rrrsrrrrnrnrrvrrrnnvrerrrnnrnvevrrnarnrsnennvnes
49. nual Rev 2 4 Cat no 1506400A 28 Chapter 6 Operation If one of the slots has completed the protocol but another slot in the instrument is still shaking you can turn off the audio signal as follows 1 Press shake n stop where n is the slot number that is still shaking The shaking will be paused 2 When the shaking is paused remove the thermal microplate holder from the slot that has completed the protocol The audio signal and the control LED are turned off and the heating of that slot is turned off 3 Press start The instrument continues to shake until the programmed time of the other slots in the instrument has elapsed If you want to stop the heating and the shaking but do not want to remove the microplate holder yet from the instrument proceed as follows 1 Pressshake nstop The shaking of the instrument will be paused 2 Press temp n stop The heating of the slot n will stop and the green LED will turn off 3 Take the microplate holder partly out and put it back in The audio signal will turn off 6 4 4 Removing the microplate Caution Do not remove the microplate holder from the instrument while the instrument is shaking This may damage the shaking mechanism 1 Wait until the slot s control LED is green and you hear the audio signal The processing protocol is complete but the heating of the slot is still on If you wish to turn the heating off press the slot number 1 3 and then press stop ha
50. nvironment where potentially damaging liquids or gases are present 5 25 Things to avoid Do not smoke eat or drink while using the iEMS Incubator Shaker HT Wash your hands thoroughly after handling test fluids Observe normal laboratory procedures for handling potentially dangerous samples Use proper protective clothing Use disposable gloves Ensure the working area is well ventilated Never spill fluids in or on the equipment Thermo Scientific EMS Incubator Shaker HT User Manual Rev 2 4 Cat no 1506400A 15 Chapter 5 Installation 5 2 6 Technical prerequisites Place the instrument on a normal laboratory bench The net weight of the instrument is 15 kg 33 lbs The instrument operates at voltages of 100 120 Vac 220 240 Vac and the frequency range 50 60 Hz 5 3 Setups before you put the instrument into operation 5 3 1 Voltage selector The voltage selector fuse block assembly Fig 5 1 is located on the left hand side Fig 4 1 item 8 of the instrument Check that the selected voltage corresponds to the local voltage You can see the current voltage setting through the slit in the fuse holder Fig 5 2 item 3 To change the selected voltage 1 Remove the power cable from the power inlet 2 Use a small flat screwdriver to pry the voltage selector fuse block assembly away from the power entry port housing and remove Fig 5 1 amy Fig 5 1 Opening the voltage selector fuse block assem
51. oplate oooonnnncncccnnnnnccnnnnonnncnnnnnnnnnnnnn nn nnn rra nnnn rr 29 6 5 Preheating and heating xs morir tr ide EAN tr a di ai di 31 6 6 Saki cid ii a ais 32 6 7 Verifying incubation and shaking parameters wrssrrrvrranarrernrnarrerrsnsrrerersnsrreessnsrreesennn 33 Thermo Scientific EMS Incubator Shaker HT User Manual Rev 2 4 Cat no 1506400A 4 7 1 7 1 1 7 1 2 7 2 7 3 7 4 7 4 1 7 4 2 7 5 7 6 8 1 8 2 8 2 1 8 2 2 8 2 3 8 2 4 8 2 5 8 2 6 8 3 9 9 1 9 2 9 3 9 4 9 5 10 10 1 11 11 1 12 13 13 1 Appendix A Appendix B Appendix C Appendix D Thermo Scientific EMS Incubator Shaker HT User Manual Rev 2 4 Cat no 1506400A Table of Contents E ae EEA TERE TEE AAE T AE a ART 34 Regular and preventive maintenance occcccccccnnconnnoncnnnnnnnnnnnnnn cnn nnnnn nana nr rr nr rre 34 Ce eee ee 34 immediates ame a e a AA ken 34 How to replace FUSES etenee ien ae 35 Disposal of materialS serioa aE EEE E E dh 36 Decontamination procedure cococcccccnnccononononcnnnnnnnnnnnn conc cnn nn nena rn rre 36 How to decontaminate the instrument SUITACO oooocccccnononcnnnaninnnnnanonnnnnnnannnnnnnnnnnnnnnns 37 How to decontaminate the thermal microplate holder rrrrrrnrvvrrnnnernrrnnnnrarnnnnennenn 37 How To pack TOF SEIVICe iria da plc dit 38 Disposal of the instrument marsvvvvravvvvvrrannvrvvnrannvnvnssnsnnvnesnsnnnnssssnnnvnssnsnnvnsssennvnessennnne 38 Troubleshooting Guide snvr
52. oves 2 Absorb the spill with disposable towels 3 Dampen a disposable towel with 2 glutaraldehyde containing decontaminating solution and clean the surfaces of the thermal microplate holder 4 Place the disposable towels into a biohazard container If the abovementioned cleaning procedure is insufficient because of the extensiveness of the spill of infectious agent perform the following decontamination procedure 1 Place the thermal microplate holder into a container containing decontaminating solution with 2 glutaraldehyde for 30 minutes or according to the directions provided by the manufacturer of the disinfectant solution 2 Rinse the thermal microplate holder with water 1 Ref National Committee for Clinical Laboratory Standards Protection of Laboratory Workers from Infectious Disease Transmitted by Blood Body Fluids and Tissue Tentative Guideline NCCLS Document M29 T2 Vol 11 No 14 Villanova Pa NCCLS 1991 Ref National Committee for Clinical Laboratory Standards Protection of Laboratory Workers from Instrument Biohazards Proposed Guideline NCCLS Document 117 P Vol 11 No 15 Villanova Pa NCCLS 1991 Thermo Scientific EMS Incubator Shaker HT User Manual Rev 2 4 Cat no 1506400A 37 Chapter 7 Maintenance 7 5 How to pack for service When you ship the instrument for service remember to e Inform about the use of hazardous materials e Decontaminate the instrument beforehand e Pack
53. rial no PURCHASED BY PURCHASED FROM Company Organization Distributor Department Address Address Tel Tel Fax Date of delivery Internet home page Date of purchase Your research area Dr O Mr O Mrs O Ms O Job title Position Surname block capitals First name block capitals Internet e mail address Excellent Above As Below Comments expectations expected expectations Reagent kit Instructions o O O a Instrument User manual O O O O Operational reliability O Oo Oo O Design a O O O Ease of use O O o o Operational costs O o o o Customer support O O O o Further instrument system developments desired Further applications desired Where did you first learn about the product Would you like to receive information about other Thermo Scientific products Thermo Scientific EMS Incubator Shaker HT User Manual Rev 2 4 Cat no 1506400A 56 Appendix D Addresses Appendix D Addresses For the latest information on products and services visit our websites at http www thermo com Manufactured by Distributed by Thermo Fisher Scientific Oy P O Box 100 Fl 01621 Vantaa Finland Tel 358 9 329 100 Fax 358 9 3291 0415 www thermo com Thermo Scientific EMS Incubator Shaker HT User Manual Rev 2 4 Cat no 1506400A 57 Notes Thermo Scientific EMS Incubator Shaker HT User Manual Rev 2 4 Cat no 1506400A 58 Notes
54. rom a flashing orange to a continuous orange 7 Press the slot number 1 3 and then start to start the protocol The protocol will start and the LED will light up with a red color 8 When the protocol is completed the LED will light up with a green color and you will hear a beeping sound The heating stays on 9 Toturn off the heating press the slot number and then stop 10 Remove the thermal microplate holder from the slot when the module is not shaking Warning The thermal microplate holder will be hot after use Only move the microplate holder from its handle Use protective gloves when opening the cover and removing the microplate Caution Do not place the microplate holder in or remove it from the instrument while the instrument is shaking This may damage the shaking mechanism 11 Turn the instrument OFF Thermo Scientific EMS Incubator Shaker HT User Manual Rev 2 4 Cat no 1506400A 54 Appendix B Certificate of Decontamination Appendix B Certificate of Decontamination The decontamination procedure is required prior to shipping the instrument to Thermo Fisher Scientific Oy for example for repair If for any reason the instrument is shipped back to Thermo Fisher Scientific Oy it must be accompanied by a dated and signed Certificate of Decontamination which must be attached to the outside of the package containing the instrument See Section 7 4 Decontamination procedure Failure to confirm de
55. s are designed to stop heating if temperatures reach 85 C 5 C in the unlikely event that temperature control to a microplate holder is lost or enters a runaway condition Microplate holders at this temperature will be hot when touched To help prevent loss of temperature control ensure the power contacts on the back of the microplate holder are clean and free from dirt or corrosion and that the holder is correctly inserted into the slot before you start the processing protocol 8 23 Temperature Changing programmed temperature setting When you change the programmed temperature setting ensure the slot temperature is at the new target setting before you start the processing protocol The instrument does not prevent the processing protocol from starting if the slot is not at the target temperature You can view the actual slot temperatures on the instrument display Refer to Section 6 5 Slot warmup time Allow the slots to reach the programmed target temperature before you start any processing protocol The slot has reached the target temperature when the orange LED has stopped flashing and stays continuously orange Refer to Section 6 3 Slot temperature Before you start the processing protocol ensure the temperature of the slot is within the acceptable range appropriate for the application to be carried out You can view the actual slot temperature on the instrument display Refer to Section 6 5 8 24 Shaking Module shake speed
56. s elapsed If you remove the microplate holder from the slot before the incubation time has elapsed the incubation timer does not stop and continues to count down causing tracking of actual incubation time to be lost Note Do not remove the thermal microplate holder until the incubation time 2 After the shaking and incubation have been completed you can remove the thermal microplate holder lift the handle upwards Fig 6 5 and pull out Fig 6 6 Be careful not to spill the contents of the microplate when you take the microplate holder out of the slot and when you remove the microplate from the holder microplate holder from its handle Fig 6 2 Use protective gloves when A Warning The thermal microplate holder will be hot after use Only move the opening the cover and removing the microplate o Note If one of the slots has completed the protocol but another slot in the 1 instrument is still shaking you can pause the shaking as follows Press shake n stop where n is the slot number that is still shaking The shaking will be paused When the shaking is paused remove the thermal microplate holder from the slot that has completed the protocol Press start The instrument continues to shake until the programmed time of the other slots in the instrument has elapsed Thermo Scientific EMS Incubator Shaker HT User Manual Rev 2 4 Cat no 1506400A 29 Chapter 6 Operation Fig 6 5 Removing the microplate holder lifti
57. sennnnessennnnessennrnsseenrnnessennnne 53 Thermo Scientific EMS Incubator Shaker HT Quick Reference Guide 54 Certificate of Decontamination euvnornvnnnnnnnnnnennvnnnnnnnnnennnnnnnnnnnnnnnennnnnnnnnunennnnnnnnnnununene 55 Thermo Scientific EMS Incubator Shaker HT Feedback Form 0 ccccccsseeees 56 Addresses kata ON 57 List of Figures Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig Fig 4 1 4 2 4 3 4 4 4 5 5 1 5 2 6 1 6 2 6 3 6 4 6 5 6 6 7 1 7 2 List of Tables Table 5 1 Table 6 1 Table 6 2 List of Figures and List of Tables EMS Incubator Shaker HT front VIEW itarish a e e nn 10 EMS Incubator Shaker HT rear ViW oooooonnniiccccnnnnnncccnncccn roca n rca 11 EMS Incubator Shaker HT keyboard cooooociociiicocococicinananocccconccnn non rnccrnnannn nan nrn 12 EMS Incubator Shaker HT QIisplay coooocooncconiccnccoconicananan acc aE e ae AEA ARAE EAR EEEN 12 Thermal miro plate NOEL A aii 13 Opening the voltage selector fuse block assembly oooooniininicnccninicananancnccccncananos 16 Selecting t correct VONAGE musical ti AA ali 17 PHACIDICLOF OPEN drid oie Trees pietet vade 19 Microplate in the thermal microplate holder rrrrrrrrrrrrrrrrrvrrrrrrrvrrrrrrrrrrrrrrrerener 24 Inserting the thermal microplate holder into a SIOt rrrrrrrrrrrrrrrrrrrrrrrvrrrrrrrrrrrsrreer 25 Pushing down the microplate holder to latch it properly oooiiioiiinn
58. set by the most recently started processing protocol The slots within a module shake at the speed that is programmed into the most recently started processing protocol Incorrect shaking speed may result if you start a protocol within the same module that has a shaking speed different from the other slots already in use To minimize the likelihood of this occurring it is recommended you program all processing protocols within a module with the same shaking speed Refer to Sections 6 2 and 6 4 3 3 During shaking Do not place the microplate holder in or remove it from the instrument while the instrument is shaking This may damage the shaking mechanism Thermo Scientific EMS Incubator Shaker HT User Manual Rev 2 4 Cat no 1506400A 41 Chapter 8 Troubleshooting Guide 8 25 Operation Ensure the thermal microplate holder is latched into its normal operating position Ensure you have inserted the thermal microplate holder correctly into the slot before you start the processing protocol When inserting a microplate holder into a slot push the holder completely in and down to latch it into its normal operating position If the microplate holder is not latched into position it may lose contact with the temperature controlling connectors at the rear of the slot Refer to Section 6 4 1 Instrument reset due to power loss If the power to the instrument is lost the instrument will reset to the READY mode when the power is restored All
59. shaking and incubation functions including timing will be turned off No LEDs will be lit and the current time of day will be shown on the display During shaking Do not place the microplate holder in or remove it from the instrument while the instrument is shaking This may damage the shaking mechanism 8 2 6 Maintenance AN Turn power off and unplug instrument during maintenance procedures To prevent electric shock turn the power off to the instrument and unplug the power cable during maintenance procedures Dirty thermal microplate holder contacts Ensure the power contacts on the back of the thermal microplate holder are clean and free from dirt or corrosion Dirty contacts may affect temperature control of the microplate holder in the slot Corrosive materials Do not use corrosive materials during maintenance procedures Refer to Chapter 7 Maintenance in this manual for information on maintenance procedures Painted surfaces can be cleaned with most laboratory detergents Dilute the cleaning agent as recommended by the manufacturer Do not expose painted surfaces to concentrated acids or alcohols for prolonged periods of time as damage may occur Decontamination Microplate holders will be hot when touched after use Allow the holder to cool down prior to performing the decontamination procedure If any surfaces have been contaminated with biohazardous material a mild sterilizing solution should be used See Section 7 4
60. srrvnnanavvvnnannvvvnnannvvenrsnsrvennsnsrrenessnsrrenssnsnvenens 15 5 2 4 Climatic requirements iii dada 15 5 2 5 TRINOS TO AMO lis ta nata 15 5 2 6 Technical prerequisite cria a Feen eten 16 5 3 Setups before you put the instrument into OperatiON ooocccconcccncnancnnnnnonananncnnnnnnnncnnnos 16 5 3 1 Voltage Selector di 16 5 3 2 Mains supply Cable iia a ae oes 17 5 3 3 SOLU renne sense mea bena lande 18 6 Operation AO 19 6 1 Principle of OperatiON oonnccccnnnoccccnnononnnonononn conan nn EEREN A EASA EEE EENE EEEE 19 6 2 Programming Protocol Sanar ad a o aaa ober vat devin aE a a A ia 20 6 3 Slot status control LEDs and audio SigNal ooooccnonocccccnononcnnnanoncnnnnnnnnnnnnnnonnnnnnnrnnnnnnnos 23 6 4 Normal OperatiO atte cani AT 24 6 4 1 Placing the Microplate cto bo 24 6 4 2 Before pressing Start ua manken 26 6 4 3 Operation vias ei ti ot EE EAS 27 6 4 3 1 Starting the oper tionuuskssuemasanne dende AG 27 6 4 3 2 Checking the operation time rrrenannvvvnnnannvvennanavvennsnsrvennsnsvvenssssvnenssnsrnneessnsrveesssnveeen 27 6 4 3 3 Starting a program during operation in another SlOt srrreranavvvnnanavvvnnannnrvnnssnsvvnnenn 27 6 4 3 4 Checking the operation stop time rrrmnassrvvrnavavvvnrarsvvvnrrrnnnnvnrsssnnvnesnsnnvnesnsnnrnssssennnne 28 6 4 3 5 Stopping the operatios serr e Erne R EN AE cnn REKE AI RE ENANA EAA REKE EIERS E AAA RETENE 28 6 4 3 6 Turning off the audio signal asss TE E E 28 6 4 4 Removing the micr
61. ssipation 785 BTU max Fuses 2 x 3 0 A 250 V UL198G Time Delay 5 x 20 mm IEC 127 3 IIl Operating conditions indoor use 10 C 40 C RH 90 Transportation conditions 40 C 70 C packed in transport packaging Storage conditions 25 C 50 C packed in transport packaging Microplate dimensions Maximum 86 mm W x 128 mm L x 15 mm H Device interfaces 1 Serial RS 232C interface for computer 2 Serial RS 485 interface for other device link Thermo Scientific EMS Incubator Shaker HT User Manual Rev 2 4 Cat no 1506400A 44 9 2 Incubator specifications Chapter 9 Technical specifications Performance of the incubator with flat bottomed microplates with nominal voltages Programmable temperature range 14 C 69 C Controlled incubation range Ambient plus 3 C to 69 0 C Resolution 0 1 C Programmable incubation time Up to 48 hours in steps of 1 second Slot warmup time lt 35 minutes to reach a 65 C 1 0 C target temperature when starting from ambient temperature approx 24 C on an average 95 of setting value with 200 ul water well with a plate sealer at nominal voltage Inaccuracy Uniformity 0 5 C calculated as the mean of the minimum and maximum well temperatures of wells A1 A12 H1 H12 and E7 after 1 hour at 65 C with an ambient temperature of 24 C with 200 pl water well with a plate sealer an
62. t e If the programmed target temperature is lower than the actual temperature of the slot Incubator slot preheating When you power on the instrument heating of the incubator slots does not occur automatically You must turn the temperature function on Preheating after you have powered on the instrument Refer to Section 6 5 Module incubation temperature All slots within a module will be heated to the same temperature The slots within a module are kept at the temperature that is programmed into the first started processing protocol in the module If another temperature is programmed to another slot in the same module the program cannot be started The message PROHIBITED OPERATION will display Refer to Section 6 4 3 Module shake speed All slots within a module will shake at the same speed The slots within a module shake at the speed that is programmed into the most recently started processing protocol Refer to Section 8 2 4 Do not place the microplate holder in or remove it from the instrument while the instrument is shaking This may damage the shaking mechanism 8 2 Warnings and cautions 8 2 1 Installation When unpacking the instrument it is recommended two people lift the instrument together taking proper precautions to avoid injury Do not touch or loosen any screws or parts other than those specially designated in the instructions Doing so might cause misalignment and will invalidate the instrument warranty
63. to select the setup menu 2 Select 1 for clock Press Enter 3 Program the new time hh mm ss and press 4 24 hour clock The baud rate is used for service purposes only If you want to interrupt the setup press stop to return to the READY state Thermo Scientific EMS Incubator Shaker HT User Manual Rev 2 4 Cat no 1506400A 18 Chapter 6 Operation 6 1 Principle of operation When you power on the instrument it will be in the READY state From the READY state you can choose to modify the setup parameters Section 5 3 3 Setup or the protocol parameters Section 6 2 Programming protocols If you have already programmed protocols while in the READY state you can start to preheat the required slots Section 6 5 Preheating and heating before starting the operation Section 6 4 3 Operation It is also possible to start shaking the slots without heating as a separate function Section 6 6 Shaking For an overview of the operations see Fig 6 1 below Slot requires Start preheating protocol Change protocol i parameters Shaking only Slot no 1 3 0r0 for all slots Slot no 1 30r0 Slot no for all slots 1 30r0 for all slots HEAT ON Slot no 1 3 0r0 for all slots Enter Protocol starts Set protocol parameters see 6 2 SHAKING ON Fig 6 1 Principle of operation Thermo Scientific EMS Incubator Shaker HT User Manual Rev 2 4 Cat no 1506400A 19 Chapter 6 Operat
64. to well do not overfill wells for a given shaking speed setting When the well is filled to 50 of its nominal volume the shake speed setting should not exceed value 4 It is recommended to use the setting 3 in most cases Program the shaking speed by pressing the appropriate number key corresponding to the required speed For example for a speed of 900 rpm Press 3 J Enter If no shaking is desired select the value 0 In this incubation module shaking is now disabled in every slot If you try to start a slot with a protocol with shaking an error message will appear on the display On the display the READY state will appear after the programming phase SHAKE SPEED O rpm VALUE 012345 SHAKE SPEED 900 rpm 012345 VALUE PLACE 2 PROHIBITED OPERATION 09 26 15 Thermo Scientific EMS Incubator Shaker HT User Manual Rev 2 4 Cat no 1506400A 22 Chapter 6 Operation 6 3 Slot status control LEDs and audio signal Each slot of the EMS Incubator Shaker has a control LED light emitting diode that displays the status of the slot The LED will change to a specific color based on the status of the slot An audio signal is given when the green LED is turned on see Section 6 4 3 6 The colors of the LED are defined in the table below Table 6 2 LED color codes Color code Explanation Flashing orange O The heating has been turned on but the set temperature has not yet been reached
65. urn to the READY state Your changes will be saved Thermo Scientific EMS Incubator Shaker HT User Manual Rev 2 4 Cat no 15064004 20 Chapter 6 Operation Program the incubation time by dialing the number keys first hours then minutes and finally seconds o Note Check where the cursor underline is located before you enter a value I1 The value will be entered at the location of the cursor For example for an incubation time of 1 hour 30 minutes Press 01 30 00 J INC TIME 01 30 00 SHAKE TIME 00 00 00 Note If you want to interrupt the programming press Stop to return to the 1 READY state Your changes will be saved Program the shaking time by dialing the number keys first seconds then hours and finally minutes o Note Check where the cursor underline is located before you enter a value 1 The value will be entered at the location of the cursor For example for a shaking time of 20 seconds Press 00 00 20 J SHAKE TIME 00 00 20 INT TIME 00 00 00 Note If you want to interrupt programming press StOp to return to the 1 READY state Your changes will be saved Interval time is a programmable parameter that allows you to specify at what interval the shaking is alternately switched off and on for example an interval time of 00 00 10 results in 10 seconds of shaking turned on 10 seconds of shaking turned off 10 seconds of shaking turned on etc for the duration of the programmed shaking time

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