WellChrom Preparative HPLC Pump K
Contents
1. 2 Usethe START STOP Key see Pos in Fig 2 on page 7 to start and stop solvent delivery SOP Purging the HPLC Pump K 1800 This SOP applies to the HPLC Pump K 1800 Firmware Revision 1 0X 1 Openthe De aeration capillary purging outlet Pos by loosening the De aeration screw Pos prior to starting the purging function to avoid damaging your column system _ EE CAUTION Purging without opening the purging outlet may cause damage to your column and system 2 Press purge key see Fig 2 on page 7 for start and stop of purging The pump is purged with the currently active Low Pressure Gradient solvent composition according to SOP Setting the Solvent Composition on page 20 Select a flow rate for purging If LPG is selected purging can be performed in mode MIX using the programed solvent gradient Select the desired mode or source by positioning the cursor on the appropriate position MIX A B C Or D Load Edit Prog Flow Pressure Events Prog Clear0 ml min 0 1 MPa offo oni J2 Link lt 030 0 000 gt Setup viw lt PURGE MIX A B C D per Hold 0 Time A B C D Run 1 min Fig 14 Display while purging in LPG mode SOP Piston Backflushing Backflushing the piston removes traces of salt and other decontami nants from the backside of the pistons Especially if you use saline solvents or buffer solution we highly rec ommend that you continuously backflush in order to prevent crys t2. M usterexem plar WellChrom Preparative HPLC Pump K 1800 User M anual V 11 98 0 oo o 00000 Table of Contents SOPs in this Manual General Description Using this Manual Setup of the HPLC Pump K 1800 Unpacking Packing List Identifying installed Pump Heads Front View of the HPLC Pump K 1800 Pump Heads and Hydraulic Connections Rear Panel View of the HPLC Pump K 1800 Connection to Mains Power Control of the HPLC Pump K 1800 Power On and Selftest Features of the User Interface of the HPLC Pump K 1800 Appearance of the Cursor Menu structure of the HPLC Pump K 1800 Using the Arrow keys in the SETUP Menu Using the SETUP Menu Using the GLP Menu Using the LINK Menu Using the VIEW Menu Operating the HPLC Pump K 1800 Working Example for setting parameters Using the High Pressure Gradient Mode Direct Control of the Pump Piston Backflushing Programing the HPLC Pump K 1800 Features of Programs Using Program Links Connecting the HPLC Pump K 1800 to Other Instruments Hydraulic Connections Single Connection 250 ml Head 1000 ml Head Electrical Connections Using the EVENT and ANALOG ERROR START Sockets Connecting Plug Strips Connecting the Event Socket Specifications of the Event Signals Connecting the ANALOG ERROR START Socket Using the RS 232 Serial Interfaces Easy Maintenance Changing and Cleaning the Pump Head List of Error Codes List of Spare Parts and Accessories Declaration of Conformity Gu
3. 4 15 06 98 GLP Last service 00 00 00 code O GLP Power index 00007 8 MPah GLP Total volume 4 1 2 1 GLP VERSION 1 0X Ser No 12345678 Overview of the GLP menu ERR 12 ERR 13 Date Time Date Time 13 11 98 16 21 33 04 11 98 09 58 52 1 E uds Lp For an overview of error codes refer to chapter List of Error Codes on page 40 Fig 10 Using the LINK Menu The LINK menu reports status information of the currently active pro gram links It is activated only if a link is running No LINK running LINKS P program lt P Link lt 21 29 R repeat View lt Main Menu LINK is running Main Menu L1 No01 P01 R 05 1 gt Link lt RUN No02 P13 R10 NY view lt 1 L1 No02 P13 R 09 A RUN No03 P01 R10 v Vy L1 No03 P01 R 02 A RUN Block diagram of the LINK menu The first line of the menu gives the current status of the links The field R xx shows the number of program runs that have to be per formed The value of R xx is decreased according to the remaining runs The second line shows the next step of the link to be performed Up and down arrows at the right hand side of the display indicate exist ence and position of additional program steps Using the VIEW Menu This menu lists existing programs and program links An example is given in Figure 11 Programs 1 2 3 4 11 and link 21 have been created Control of the HPLC Pump K 1800 is
4. Part Ain Fig 17 on page 29 is showing that step with cursor blinking at No Part B in the figure is showing that programing step Load Edit Prog Flow Pressure Events Prog ClearO ml min 0 1 MPa offo oni J2 Link lt 01 L2 No View lt OFF Hold 0 Time A B C D Run 1 min Load Edit Prog Flow Pressure Events Prog ClearO ml min 0 1 MPa offo oni JL2 Link lt j 01 L2 No01 P07 RO5 WaitdO D setup View lt j OFF No P R Wait pair Hold 0 Time OA Y B C D Run 1 min Display while editing a program link During execution of a link the LINK menu is available in addition to the common display In the LINK menu the momentary status of the running link is shown Move cursor to the Load Prog field and press lt to enter this menu page For more information refer to Fig 10 on page 17 Press gt to exit link menu Executing Program Links This SOP applies to the HPLC Pump K 1800 Firmware Revision 1 0X 1 Enter the desired link number into the field Load Prog and confirm by pressing any arrow key 2 Press START to start the pump The display switches to ON Programing the HPLC Pump K 1800 30 tual Lo CAUTION Bac Lap 3 Position cursor on field ON 4 Start the link by pressing numeric key 1 The display switches to RUN After execution of a link the HPLC Pump K 1800 stops solvent deliv ery the display switches to OFF Connecting the HPLC Pump K 1800 to Other Instruments Hydraulic
5. These can be accessed by authorized mainte nance personnel only PRESSURE LIMITS Preset for the minimum and maximum system pressure The pump will switch off automatically if the system pres sure exceeds limits If minimum pressure is set to 0 no check of the minimum pressure is carried out The specified maximum pressure applies to all modes of operation and can never be exceeded Units 0 1 MPa 15 uc Lage START INPUT If the START INPUT is set to ENABLED a program can be started by an external digital signal from the START INPUT on the rear side of the pump see chapter Connecting the ANALOG ERROR START Socket on page 33 In the setting STOP Pump an external digital signal from the START INPUT will stop the pump if it is pumping with the preselected flow rate The setting START Pump will start pumping on picking up the external signal Having set START INPUT to START Pump or STOP Pump no program run will be started and control by network is inactivated DATE TIME You can use this menu for setting the system date and time of your HPLC Pump K 1800 CONTROL The instrument can be set to different external control modes Options are KNAUER NET where the baud rate is currently limited to 9600 RS 232 RS 485 and ANALOG Having selected ANALOG the current flow rate will be indicated and you can put in an offset correction or do an input signal scaling respectively Move cursor to Offs and press V then move to Flow
6. 20 minutes This program is based upon the configuration in section Operating the HPLC Pump K 1800 on page 18 This setup must be performed before program definition Remember a dash indicates that calcula tion of the corresponding parameter is continued 1 Select the operation screen 2 Movecursor to the field Edit Prog press 7 to generate program 07 3 Press Y to enter the Edit mode for the new program The cursor is positioned at Time 000 0 Programing the HPLC Pump K 1800 28 SOP 4 Press A to setthe flow rate Press 1 0 O and then V to enter the value 100 0 ml min The display should look like part A see Fig 15 on page 25 showing the cursor blinking in the Time field 5 Press gt to enter field A then 9 and O and lt to confirm the input The amount for B is automatically calculated and indicated as 10 The display should now look like part Bin the figure 6 Pressnow V again to put in the next program line by defining a new time step The display now should look like part C in the figure having the cursor blinking on the first asterisk 7 Now press 2 and 0 and then gt to define time step 20 0 min as well as to enter field A 8 Pressland 0 for 10 A and then lt 4 to confirm the input The amount for B is automatically calculated and indicated as 90 Part D in the figure is showing the current situation This program can be executed Using Program Links Program links are combination
7. Connections Make sure that all hydraulic connections are suited for the sys tem pressure and flow rate of your HPLC System Single Connection 250 ml Head Solvent tubings are connected to the pump head according to Figure 18 Pump heads Hydraulic connections Make sure that the flat side of the ferrule is directed to the pump head Make sure that the fitting screw is fastened hand tight 1000 ml Head Push the tubes directly over the connectors and secure them by means of appropriate clamps Saal 3 Sues Lap 250 ml Head 1000 ml Head Tube Tube connectors Fitting screw Ferrule Fig 18 Pump heads Hydraulic connections For order numbers see Figure 21 Explosion Diagram and list of parts of 250ml and 1000 ml pump heads Electrical Connections Using the EVENT and ANALOG ERROR START Sockets of the HPLC Pump K 1800 Electrical signal lines from other instruments can be connected to e the ANALOG ERROR START or e EVENTS socket or the e RS 232 serial interface on the rear side of the HPLC Pump K 1800 see Positions i and in Fig 4 on page 9 Events can be controlled in the main menu or in programs You can use SOP Setting Events on page 21 Programing the HPLC Pump K 1800 32 311 EA CAUTION Fig 19 SOP Do not touch the electrical contacts of the socket lines Electro static discharges can lead to damage of the electronics of the HPLC Pump K 1800 Connecting Plug Strips You ca
8. K 1800 with the following procedure 1 Place the cursor in one of the four corners of the main screen e g on OFF in the lower left corner of the main menu 2 Select either right or left arrow key In the example above selection of key lt J will activate the VIEW MENU on the display Control of the HPLC Pump K 1800 121 biri aapt 3 Select the rhombus in the lower left corner of the display if you want to access additional pages of the selected menu You can access all menu pages by scrolling using the up A or down arrow keys V respectively Load Edit Prog Flow Pressure Events Prog Clearo ml min 0 1 MPa offO0 oni J2 Link lt Links 2 gt Setup View lt 21 29 __ gt GLP Hold 0 Time AC D Rhombus Run 1 min dd dd i Fig 7 Example for a multi page menu VIEW menu M enus without rhombus are single page menus SOP Entering Data This SOP applies to the HPLC Pump K 1800 Firmware Revision 1 0X Use this SOP for entering parameters and program steps in your HPLC Pump K 1800 1 Place the cursor at the desired position of the main screen 2 Press a numeric key to start data input The appearance of the cursor changes to a underline character see Fig 5 on page 10 Press numeric keys for entering desired numeric values Confirm value by pressing any of the arrow keys This finishes data entry mode and moves the cursor according to the arrow key direction 5 You can modify and delete data ac
9. and Outlet valve housing Pos and then disassem ble them according to the following instructions 1 Place the valves in a suitable cleaning solution Use a ultrasonic bath to clean the valve If malfunction persists use steps 2 5 of this SOP 2 Using aknife or similar carefully remove the valve seal from the housing see Fig 23 on page 40 3 Remove the individual components by gently tapping the housing on the table Clean the individual parts We recommend a ultrasonic bath Assemble the check valves in reverse order use Figure 23 Pay attention to the correct orientation of the valve seating see cross section in Figure 23 Easy Maintenance 40 pl Direction of flow Housing Valve seating i ball cross section Lar illustration Fig 23 Components of the check valves of all pump heads available List of Error Codes Table 4 Error codes in GLP M enu M otor failure ERROR IN signal detected Switch off due to exceeding maximum current Switch off due to exceeding maximum pressure Switch off due to falling below minimum pressure M otor blocked Calibrating values re initialized Calibrating curve re initialized Combination from 33 34 List of Spare Parts and Accessories KNAUER Order Optional Pump heads Number Pump Head stainless steel 250 ml A4021 Pump Head stainless steel 1000 ml A4022 Cables Power supply cable 230V M1642 RS 232 connection cable M1703 Set of plug strips 3x4 2
10. and put in the flow rate which is corresponding to the input voltage signal Select ing O zero will reactivate the standard setting The available ranges comprise 250 ml head from 2 5 ml min per 1 Volt resulting in a working range of 0 25 ml min to 37 5 ml min per 1 Volt resulting in a working range of 0 250 ml min 1000 ml head from 10 ml min per 1 Volt resulting in a working range of 0 100 ml min to 150 ml min per 1 Volt resulting in a working range of 0 1000 ml min limited by maximum flow rate ANALOG OUTPUT On activation of PRESSURE the output ANALOG OUT is delivering the pressure signal for external monitor ing or controlling On activting SLAVE B a second pump can be con trolled via this analogue output In this case the control signal voltage is representing the percentage amount B where A will be delivered automatically by the K 1800 itself The of voltage range can be set as 0 1 V 0 2 V 0 5 V as well as 0 10 Volts The maximum value is corresponding to 100 B or 40 MPa respectively Control of the HPLC Pump K 1800 Fig 9 Lo Main Menu Using the GLP Menu The GLP menu reports statistical data for vital parameters of the HPLC Pump K 1800 Fig 9 gives an overview of the GLP menu gt Setup Dar lt p GLP Total working time 000003 h 02 m 09 s GLP Total pump cycles 210952 GLP Last error codes Y 12 13 E GLP Installation date
11. equipped with an universal power supply for two input voltage ranges 90 130 Volts AC and 170 260 Volts AC A manual setting of the supply voltage is not required Make sure that mains power supply is properly grounded and a correct three pronged power cable is used Connecting the instrument to faulty power sockets can cause injuries Connect the power cable to the socket on the rear of the instrument see in Figure 4 and switch on the device with the ON OFF switch Pos at the front side of the device Further devices with a maximum power consumption up to 500 Watts may be connected to the Power connector line out Pos in Fig 4 and will be switched on or off simultaneously with the HPLC Pump K 1800 Control of the HPLC Pump K 1800 10 tual EAS CAUTION Fig 5 uds Lp Control of the HPLC Pump K 1800 Power On and Selftest On power on the instrument displays HPLC PUMP MACRO STAR and the VERSION of the software e g 1 0X see 1 in Fig 1 on page 6 The instrument performs a self test including firmware vali dation and tests of RAM and motor unit After completion the mes Sage SYSTEMTEST OK indicates that the pump is ready for operation and the ID of the last active program is displayed Never run the HPLC Pump K 1800 without liquid in the pump head or in the piston backflushing compartment For piston backflushing follow the instructions given in chapter Piston Backflushing on page 23 Opera
12. features a double piston design with active pulsation dampening It is capable of delivering solvents with very low residual pulsation It can be configured with two different pump heads to meet a broad range of requirements of flow ranges e 250 ml pump head 0 1 250ml min stainless steel e 1000 ml pump head 0 1 999 9ml min stainless steel Pump heads can be interchanged by loosening only four screws Experienced users can disassemble the heads for simple maintenance tasks like cleaning changing piston seals etc The easy to learn user interface of the HPLC Pump K 1800 provides access to the integrated software It supports quaternary high pres sure gradient systems via a master slave configuration where up to three additional K 1800 pumps can be controlled Most KN AUER Software Packages like EuroChrom 2000 allow for digital remote control of any configuration of KNAUER solvent deliv ery systems enabling full and transparent integration of the HPLC Pump K 1800 pump into a wide range of chromatographic systems Using this Manual The captions in this manual referring to individual items in figures are identified with by format like see Pos in Fig 1 on page 6 which identifies the pump head Outlined arrows like Jb used in block diagrams indicate an auto matic program run and change to the next line without any need of manual input Arrows like 4 gt used in block diagrams indicate that the user is asked to pre
13. to use the Direct Control M ode you need to specify e Maximum system pressure e Flow Rate and e Solvent composition if working in LPG or HPG gradient mode Setting the Maximum Pressure In addition to the maximum pressure specified in the SETUP menu see Using the SETUP Menu on page 13 by either the pump head or the global pressure limit you can specify a maximum system pres sure for the currently active mode of operation or the currently active program This SOP applies to the HPLC Pump K 1800 Firmware Revision 1 0X 1 Movethe cursor to the parameter PRESSURE see in Fig 2 on page 7 2 Type in the desired setting for maximum system pressure The selected value is marked with two vertical lines which indicate that this is a programed instead of a measured value 3 Press any arrow key for confirmation and new selection Setting the Flow Rate This SOP applies to the HPLC Pump K 1800 Firmware Revision 1 0X Use this SOP for setting the correct flow rate 1 Position cursor on field FLOW see in Fig 2 on page 7 2 Press any numeric key to select desired flow rate You can use information given in section Appearance of the Cursor on page 10 and SOP Entering Data on page 12 3 Press any arrow key for confirmation and new selection Setting the Solvent Composition This SOP applies to the HPLC Pump K 1800 Firmware Revision 1 0X 1 Movethe cursor to the field A see in Fig 2 on page 7 2 Typein the desired m
14. ul Fig 11 A CAUTION uds Lp Programs 01 02 03 04 __ Link lt 01 10 a eS R R lt 1 View lt 1 Main Menu Programs 4 11 20 The VIEW menu with programs 1 4 11 and link 21 Operating the HPLC Pump K 1800 You need to setup your pump prior to operation You can use the information in sections e Menu structure of the HPLC Pump K 1800 on page 10 e Using the SETUP Menu on page 13 e Using the GLP Menu on page 16 e Using the LINK Menu on page 17 e Using the VIEW Menu on page 17 Working Example for setting parameters For your convenience this example points out how to configure a binary low pressure gradient using valves A and B This example may not comply with your needs and is given for convenience only You need to select parameters that meet your requirements Using this example without proper adjustment of parameters may lead to damage of your equipment Enter the SETUP menu see Fig 8 on page 13 Selection of correct pump head 1 Select menu pump head by pressing V Fig 12 3 Use and A to scroll through the list of pump heads until the installed type of pump appears e g 250 ml steel Selection of a pump head automatically adjusts proper maximum system pressure for the selection Press lt 1 to confirm selection and return to the rhombus Selection of operation mode 1 2 Return to page GRADIENT MODE by pressing A Press gt and
15. allization which can damage your piston seals For best protection and error free long time operation of the pump head it is recommended always to backflush the piston with an appropriate solvent according to the following SOP Preparing your HPLC Pump K 1800 for back flushing This SOP refers to the HPLC Pump K 1800 1 Push a 1 16 ID tube onto both flushing opening see Piston backflushing capillaries 1 16 connected by tube Pos in Fig 3 on page 8 Place the low end of the tubing in a flask Fill the priming syringe with priming liquid Connect the syringe with the tubing U Bb U N Press liquid through the pump head until it flows without any air bubbles into the container 6 Remove tubings from flush opening If you want operation with continuous backflushing you can attach two containers of rinsing liquid instead of the priming syringe The containers should be positioned that one container is located higher than the other thus ensuring liquid flow through the pump head without any assistance We recommend connecting both flush openings with a tubing to prevent vaporization of solvents and dry out of the piston chamber Programing the HPLC Pump K 1800 24 T SOP bari aapt Programing the HPLC Pump K 1800 Programs can perform the following actions e Determining and setting flow rates e Determining and setting solvent compositions e Control of signal events The HPLC Pump K 1800 can store up to twe
16. alternating from one to the other approx half a turn This prevents the pump head from tilting and becoming damaged Once the spring tension has been reduced hold the pump head firmly in one hand while removing the screws completely with the other hand Carefully remove the pump head Removing and Checking Piston Rods This SOP refers to the following pump heads 250ml steel KNAUER order number A 4021 1000m steel KNAUER order number A 4022 Remove Pump head as described in SOP Removing the Pump Head Easy Maintenance 36 a i Mudo Lap If you only wish to check the piston rods you don t need to disassemble the pump head any further 2 Any Piston rods Pos in Fig 21 on page 37 may be removed using pliers Take the tip of the piston using the pliers and pull it out carefully in a straight line TIP If the rods are broken you must check the entire pump head for damage SOP Disassembling the Pump Head This SOP refers to the pump heads 250ml and 1000ml 1 Apply SOP Removing and Checking Piston Rods on page 35 2 Loosen the two Retaining plate screws Pos half a turn alternating from one to the other to avoid damaging the Retaining plate 3 Because the two screws are very tight it may be helpful to either clamp the pump head or to press one of its side surfaces against a table with one hand while loosening the screws 4 Unscrew the two screws strictly alternating due to strong force of the S
17. anges to ON and the pump begins continuous operation with the parameters specified in the first program line Programing the HPLC Pump K 1800 26 T SOP SOP biri aapt Position cursor on field ON Start the program by pressing the numeric key 1 The display changes to RUN 5 During execution of programs you can press key O to freeze the current status of the pump The display changes to HOLD and the field Time is blinking 6 Ifyou press 1 you can activate program execution again or you can press STOP to abort execution During program execution the current solvent composition can be observed in fields A and B At the end of the program the pump returns to the first line of the currently active program and continuous pumping ON is displayed Modifying and Deleting Programs This SOP applies to the HPLC Pump K 1800 Firmware Revision 1 0X Use this SOP for modifying and deleting programs 1 Enter the number of the program to be modified in field Edit Prog Locate the value in the program you want to modify Enter new values Old values will be overwritten Wrong entries for example due to typing errors can be corrected the same way 4 Confirm your entries by pressing any arrow key Delete program lines as follows 5 Enter 0 zero in the Time field and press a arrow key The Message Delete this line Confirm by cursor appears Pressing any numeric key or no action for a time period S60s aborts the process of d
18. arantee Statement Service Department and Hotline O O 0 0AA A A W biri apt SOPs in this manual SOPs in this Manual The Standard Operating Procedures provided with this manual offer a con venient way of structuring complex tasks in the operation of your HPLC pump They include step by step instructions that lead the user through all routine tasks during operation They can be used for documentation pur poses and be copied applied signed and filed in order to document the performance of the instrument IMPORTANT Please operate the instrument and all accessories according to instructions and SOPs in this manual This ensures proper results and longevity of your equipment Selecting Menus 11 Entering Data 12 Selecting a Gradient Mode 14 Setting the M aximum Pressure 20 Setting the Flow Rate 20 Setting the Solvent Composition 20 Setting Events 21 Start and Stop of Solvent Delivery 22 Purging the HPLC Pump K 1800 22 Preparing your HPLC Pump K 1800 for back flushing 23 Creating a Program 24 Execution of Programs 25 Modifying and Deleting Programs 26 Using a Wake Up Program 26 Creating Program Links 28 Executing Program Links 29 Connecting Plug Strips 32 Removing the Pump Head 35 Removing and Checking Piston Rods 35 Disassembling the Pump Head 36 Assembling the Pump Head 38 Installing the Pump Head 39 Cleaning and Replacing Check Valves 39 General Description 4 pil ace Lap General Description The HPLC Pump K 1800
19. cording to SOP Modifying and Deleting Programs on page 26 Using the Arrow keys in the SETUP M enu Figure 8 Structure of the SETUP menu on page 13 gives a complete overview of the SETUP Menu e You can use gt for accessing menus e You can use A and V for scrolling through the list of parameters of any individual menu item a _ _ _ _ Mu Lap Using the SETUP Menu D setup F Y gt GRADIENT MODE none gt aLP 4 Main Menu PwWaAaAdDS PUMP HEAD 250 ml steel raservad option 009 ml steel reserved option PRESSURE AUTOZERO Offset 00 28 MPa f FLOW Adjust Correction gt 5000 19 5 Y PRESSURE LIMITS 0 1 MPa min OOO max 150 START INPUT ENABLED 0 DESABUED STOF Pump START Pump DATE 28 10 98 dd mm yy TIME 08 31 10 hh mm ss CONTROL KNAUER NET Baud rate 9600 a P Ro aa ANALOG lt CONTROL ANALOG Offs 00mV Flow 220 0ml ANALOG OUTPUT Pressure Range 1 v Slave B Fig 8 Structure of the SETUP menu Control of the HPLC Pump K 1800 14 ul SOP biri aapt The SETUP menu of the HPLC Pump K 1800 enables access to the following features and functions GRADIENT MODE The HPLC Pump K 1800 is capable of isocratic none high pressure gradient HPG and low pressure gradient LPG operation You can choose the mode of operation Selecting a Gradient M ode This SOP applie
20. cording to SOPs Solvent composition if working in LPG or HPG gradient mode on page 20 Setting the Flow Rate on page 20 Setting the Solvent Composition on page 20 or Setting Events on page 21 Load Edit Prog Flow Pressure Events Prog Clear 0 ml min 0 1 MPa offO0 oni J2 Link lt 1 01 07 100 0 200 00000000 M setup A vew lt OFF 000 0 100 O gt gt GLP Hold 0 Time on A B AC D Run 1 min Load Edit Prog Flow Pressure Events Prog Clear0 ml min 0 1 MPa offo oni JL2 Link lt j 01 07 100 0 200 00000000 fp setup B view lt OFF 000 0 90 10 gt gt GLP Hold 0 Time oA B C D Run 1 min Load Edit Prog Flow Pressure Events Prog ClearO ml min 0 1 MPa offo oni J2 Link lt 01 07 200l gt Setup C vew lt lt OFF x x x gt GLP Hold 0 Time on A B C D Run 1 min Load Edit Prog Flow Pressure Events Prog ClearO ml min 0 1 MPa offo oni J2 O Link lt 01 07 200 M gt Setup vew lt OFF 020 0 10 gt gt GLP Hold 0 Time A B C D Run 1 min Fig 15 Display of single steps when creating a program 7 Position cursor on time field and press VY for creation of an additional time step SOP Execution of Programs This SOP applies to the HPLC Pump K 1800 Firmware Revision 1 0X 1 Load the program you want to execute Enter the desired program number in the Load Prog field and confirm by pressing any arrow key 2 Press START key to start the pump The display ch
21. crews Pos 3 19 press the plate down with one hand and tighten the screws with a screw driver strictly alternating due to strong force of the Springs Pos 9 Carefully insert the piston rods see Piston rods Pos without bending or quenching the rods 10 The Retaining plate screws Pos must be tightened that they are seated as securely as before al 39 bro Leap SOP Installing the Pump Head This SOP refers to the HPLC Pump K 1800 and the following pump heads e 250 ml steel KNAUER order number A 4021 e 1000ml steel KN AUER order number A 4022 1 Make sure that the pump head is properly assembled Position the head in a straight line onto the pump housing Tighten all four Pump head set screws Pos a few turns by hand 4 Alternating from one to the next tighten two diagonally opposed screws half a turn at a time until the pump head is correctly seated Tighten the two other screws M ake sure that all four Pump head set screws Pos are securely tightened 7 Install the two Connection capillaries Pos 3 8 beginning with the s shaped one and firmly tighten all fittings SOP Cleaning and Replacing Check Valves This SOP refers to the pump heads 250ml and 1000ml If the check valves become dirty they will no longer open and close correctly You can remove the check valves for cleaning from the pump head after loosening and removing the Inlet valve housing Pos
22. d RS 232 interface the right one of the next device and so on The ring is closed by connecting the RS 232 interface of the last device to the free interface of the first one For connections with a PC you need a Y cable which is part of the EuroChrom 2000 Software Hardware package A B PC RS 232 D Fig 20 Connection scheme for network of three instruments A Networking three pumps B Connection with Y cable and Personal Computer SOP Eo CAUTION SOP Easy Maintenance Changing and Cleaning the Pump Head Removing the Pump Head This SOP refers to the HPLC Pump K 1800 For exchange of the pump head or for disassembling it in order to clean valves or replace piston seals piston rods etc it can easily be removed Purge the pump head with a suitable cleaning agent and then with distilled water You can use SOP Purging the HPLC Pump K 1800 on page 22 If organic solvents remain in the pump head danger of skin irri tation may exist Remove the two solvent tubings from the Eluent inlets Pos in Fig 3 on page 8 Loosen the two Eluent outlets to transducer Pos and the two Inlets to transducer Pos Remove the two Connection capillaries Pos 3 8 beginning with the upper one n shaped one Using a hexagonal spanner no 4 4 mm loosen just two diagonally opposed Head set screws Pos Remove the SCrews Carefully loosen the two remaining screws
23. eletion 6 Selection of any arrow key deletes the program line Deletion of programs or program links 7 Select field Edit Prog 8 Enter 0 in the field Edit Prog 9 Confirm deletion according to steps 5 to 6 of this SOP Using a Wake Up Program This SOP applies to the HPLC Pump K 1800 Firmware Revision 1 0X The HPLC Pump K 1800 can execute a program at a preset time Fig 16 TIP Make sure that date and time of the HPLC Pump K 1800 are set up properly according to section Using the SETUP Menu on page 13 You can create a Wake Up Program as follows 1 Enter the reserved program number 30 in the field Edit Prog 2 Enter desired commands according to Creating a Program on page 24 3 Load reserved program 30 While waiting for the programed time the display is blinking The pump will start program execution with selected program at preset time and switch into RUN mode Load Edit Prog Flow Pressure Events Prog ClearO ml min 0 1 MPa offo oni J2 07 30 WAKE P 01 at 09 15 gt Setup UP on 13 06 98 gt ar Hold 0 Time on A B C D Run 1 min Display of wake up program 4 You can abort execution of Wake Up Program if you enter the number of another program Example for creating a program Generation of program 07 with the following functions Controlling solvent gradient with constant flow rate of 1 0 ml min Composition at Start 90 A and 10 B change of composition to 10 A and 90 B during
24. entering data and control the execution of programs Detailed information is given in chapter Control of the HPLC Pump K 1800 on page 10 and Programing the HPLC Pump K 1800 on page 24 Setup of the HPLC Pump K 1800 8 yl Pump Heads and Hydraulic Connections All hydraulic connections are located on the front panel of the HPLC Pump K 1800 4 2 3 Pump Head 4 Pressure Transducer 3 1 Piston backflushing capillaries 4 1 Inlets to transducer 1 16 connected by tube 4 2 De aeration capillary purging 3 2 Hluent inlets outlet 3 3 Head set SCrEWS 4 3 Deaeration screw 3 4 Label indicating maximum 4 4 Eluent outlet to column pump volume per minute 3 5 Inlets valve housing 3 6 Outlets valve housing 3 7 Huent outlets to transducer 3 8 Connection capillaries Fig 3 Pump head and pressure transducer far Laspk Rear Panel Elements 5 1 Ventilator 5 2 Terminal strip ANALOG ERROR START connec tions 5 3 Socket prepared for Ether net connection 5 4 RS 232 Interfaces 5 5 Terminal strip EVENTS connections 5 6 Power connector line in 5 Fuse compartments 5 8 Power connector line out 5 9 Plug for interconnection of optional low pressure gradient unit 5 10Serial number Fig 4 E CAUTION e eee Rear Panel View of the HPLC Pump K 1800 k ii T iii Lrt ee BS ee Pi Pik i Hel MA 5 10 Rear Panel of the HPLC Pump K 1800 Connection to Mains Power The HPLC Pump K 1800 is
25. h manufacturer s instructions or damage caused by unautho rized service personnel render the guarantee null and void Damage caused by blockages and wear and tear parts such as fuses and seals are not covered by the guarantee Claims under this guarantee are valid only if the enclosed guarantee card is returned to us at the address shown below within 14 days of receipt of the instrument Defective pumps should be sent to the manufacturer for repairs under guarantee see full adress KN AUER instruments are properly packed so that safe delivery can be assured In any case the instrument should be unpacked and checked for visible transport damage and complete delivery immedi ately after receipt of delivery In the unlikely event of any compo nents being either damaged or incomplete please inform us at the address given above within three working days of delivery You are also strongly advised to contact the logistics firm who delivered the instrument If after carrying out the necessary control procedures we find a Shortage or defect covered by the guarantee replacement or repair at our discretion will be carried out free of charge Packing and trans port costs are borne by the purchaser Service Department and Hotline Wissenschaftliche Geratebau Dr Ing Herbert Knauer GmbH Hegauer Weg 38 14163 Berlin Germany Telephone 49 0 30 809727 62 Telephone 49 0 30 809727 26
26. ixing ratio of eluent from channel A given as percentage by volume The value in the right hand adjacent field next to your input complements your value to give a total of 100 3 Press any arrow key for confirmation and new selection SOP Setting Events Events are defined as states or changes of states of the electrical con tacts of the EVENTS socket of the instrument You can either open or close any of the contacts or use a 500ms puls You may want to control the events manually e g for testing your system For connec tion of cables use sections Electrical Connections on page 31 Connecting Plug Strips on page 32 and Connecting the ANA LOG ERROR START Socket on page 33 This SOP applies to the HPLC Pump K 1800 Firmware Revision 1 0X 1 Position the cursor at the desired event field 2 Select 0 1 2 or 3 from the following table O Off 1 On 2 Pulse 3 No change with respect to previous status Load Edit Prog Flow Pressure Events Prog ClearO ml min 0 1 MPa offO0 ont J2 01 100 0 150 01000010 p setup OFF 000 0 gt gt GLP Fig 13 Relations between event programing and event terminal strip Control of the HPLC Pump K 1800 22 41 Mudo Lap SOP Start and Stop of Solvent Delivery This SOP applies to the HPLC Pump K 1800 Firmware Revision 1 0X 1 Apply SOPs Setting the Flow Rate on page 20 Solvent composition if working in LPG or HPG gradient mode on page 20
27. let see Figure 18 Pump heads Hydraulic connections Ferrule Fitting Screw Tubing SOP Assembling the Pump Head This SOP refers to the pump heads 250ml and 1000m All positions of components refer to Figure 21 Explosion Diagram and list of parts of 250ml and 1000ml pump heads on page 37 1 Itis recommended to exchange all four piston seals located in the pump head by new ones after disassembling the pump head 2 With the open side facing downwards carefully press the Piston seals Pos into the Pump head housing front block Pos and Pump head housing basic plate Pos 3 B making sure to keep them straight Fig 22 Open side of 3 Putin the two O shaped sealings Pos 3 12 piston seal ae l l Put the two housing parts together positioning the O ring side of part B against part A Be sure that the backflushing capillaries are positioned on the top side of the head see label on front of part A for orientation 5 Insert the two Spacing bolts Pos 3 14 screw them in with the hand and tighten them firmly using the SW 10 spanner that they are seated as securely as before 6 Putin the two Distance plates Pos 3 13 position the Springs Pos and the Guides for springs Pos 3 16 7 Put the Retaining plate Pos 3 18 over the Guides for springs Pos 8 16 having the conical bore of the plate directed to outwards 8 Insert the two Retaining plate s
28. n use the plug strips with 2 3 or 4 connectors enclosed in the accessories for easy installation of electrical connections 1 lever latch A 4 gt FA a po A ma Assembling plug strips Connecting Plug Strips Use this SOP for connecting wires to the plug strips 1 Insert the rounded end of the lever latch into the square opening of the selected connector of the plug strip Press the catch down as indicated by arrow Insert the uninsulated end of the cable into the opening under the catch 4 Release the catch and remove the lever latch from the plug The cable is now firmly anchored in the plug strip Table 1 Table 2 ANALOO ERATA START Connecting the Event Socket You can control the states of individual contacts of the EVENTS socket using SOP Setting Events on page 21 Specifications of the Event Signals TTL Transistor Transistor Logic connections TTL compatible out puts max consumption 40 mA Ucemax 24 V referred to GROUND Technical specifications of the event signal connections OC Open Collector State Control Outputs connections Relay connections 0 Low lt 500 mV Inactive Contact open 1 High gt 2 V Active Contact closed Pulse gt 2 V for Pulse contacts 500 ms 2 500ms Pulse active for 500ms closed Connecting the ANALOG ERROR START Socket The ANALOG ERROR START terminal strip serves to receive or send start or error signals from to other instruments Additio
29. nally a further voltage signal is available for monitoring the system pressure or for controlling a slave pump Technical specifications of ANALOG ERROR START signal connections and picture of connector Signal Description Start connections START IN Is activated by a O Volt or low signal or a short circuit lin 10mA START OUT OC open collector output is active for 500 ms Error connections ERROR IN When receiving a O V error signal the message Error Signal was detected is displayed and the pump is stopped ly 10mA ERROR OUT OC output active until the error condition is removed ANALOG OUT Analogue voltage signal Gives measured system pressure 1 V per 40 MPa Offset for O MPa is some mV or control voltage for slave pump B ANALOG IN Analogue input signal controls flow rate default 1 V per 25ml min for 250ml head and 1 V per 100ml min for 1000ml head Programing the HPLC Pump K 1800 34 41 aap Using the RS 232 Serial Interfaces The two RS 232 serial interfaces on the rear side of the device see in Fig 4 on page 9 enable digital data transfer between the HPLC Pump K 1800 and other instruments like other pumps detec tors or a PC which runs the EuroChrom software package All the devices are communicating with each other and form an integrated network All devices are connected in a token ring in the following way The first RS 232 interface the left one of the first device is connected to the secon
30. nty programs with a maximum of 100 lines of commands You can define up to nine links between existing programs Features of Programs Units of the time display are minutes with a decimal representation of seconds i e 0 3 min 18s All programs are saved in the pump s memory and are permanently available You can enter values with 1 10 min accuracy Time is displayed in 1 100 min steps during pro gram execution Programing the solvent composition does not depend on the cur rently active gradient mode i e HPG or LPG New program lines are appended in the last line with the cursor on an asterisk You can manually rearrange lines by setting a desired time If you generate a new line all parameters are set to status No Change displayed as underscore characters see D in see Fig 15 on page 25 The No Change feature allows editing desired parameters while keep ing the processing of parameters which are not changed Creating a Program This SOP applies to the HPLC Pump K 1800 Firmware Revision 1 0X Use this SOP for entering program information in your HPLC Pump K 1800 Use Features of Programs above 1 Select the operation screen main menu 2 Position cursor on menu Edit Prog 3 Enter number of desired program 4 Press any arrow key to enter edit mode for new program The cursor is positioned at Time 000 0 fixed start time Ul Position cursor on desired field Enter desired values in the desired fields ac
31. p head 00000 l aimi Pressure transducer ON OFF switch U A W NP Fig 1 Front Panel of the HPLC Pump K 1800 For additional information on connecting the pump head see section Connecting the HPLC Pump K 1800 to Other Instruments on page 30 and Figure 18 Pump heads Hydraulic connections on page 31 Fig 2 re 1 1 1 2 1 3 1 4 1 5 1 6 1 7 1 8 Display Information Program to be loaded Program to be edited Flow rate Preset maximum or measured system pressure State of Events Go to the SETUP menu Go to the GLP menu Solvent mixing ratios for gradient mixtures 0000000 1 9 Time 1 10 Status of pump or program 1 11 Go to the VIEW menu 1 12 Go to the LINK menu 2 Foil Key Area 2 1 START STOP pumping 2 2 START STOP purging 2 3 Key pad for data input 2 4 Arrow keys Display information and foil key area in detail The display shows information about the current status of the pump such as the flow rate the measured pressure or the current status of the running program It serves as a control instrument when entering data Pumping can be started or stopped by pressing the key START STOP see 2 1 using the preset values The foil key PURGE see 2 2 allows immediate access to the purging function of the pump with the pre selected flow rate Purging has to be stopped manually by pressing PURGE again or by pressing the START STOP key You can use the keys for
32. prings Pos behind the plate and remove them Remove the Retaining plate Pos 3 18 You can now remove the Guides for springs Pos the Springs Pos as well asthe two Distance plates Pos 7 Using an appropriate tool e g a screw driver carefully lever the two Piston seals Pos out of the Pump head housing basic plate Pos 8 Using the SW 10 spanner loosen and remove the Spacing bolts Pos These bolts are seated very tightly Follow the tip given in step 3 9 Lift and remove the Pump head housing basic plate Pos 3 B and remove the O shaped sealings Pos 10 As described in step 7 remove the two Piston seals Pos out of the Pump head housing front block Pos Fig 21 Table 3 Position 3 A 3 B 3 1 3 2 3 3 3 5 3 6 3 10 3 11 3 12 3 13 3 14 3 15 Explosion Diagram and list of parts of 250ml and 1000 ml pump heads Components of the Pump Head Description Pump head housing front block Pump head housing basic plate Piston backflushing capillaries 1 16 Eluent inlets Pump head set screws Inlet valve housing Outlet valve housing Check valves inlet and outlet Piston seals O shaped sealings Distance plates Spacing bolts Springs Easy Maintenance 38 p i biri Le Table 3 Components of the Pump Head 3 16 Guides for springs 3 17 Piston rods 3 18 Retaining plate 3 19 Retaining plate screws Parts for in
33. s of existing programs which can be created and edited like any programs Links use reserved program numbers 21 29 These numbers are automatically converted like 21 L1 22 L2 etc The HPLC Pump K 1800 can store up to 9 different links Links use line numbers from No01 to No99 each line referencing a linked program The optional parameter gives the number of runs of that program A wait status can be programed The maximum num ber of programs in all links referenced is 100 Any program can be referenced multiple times in a link A program link can not reference other links Creating Program Links This SOP applies to the HPLC Pump K 1800 Firmware Revision 1 0X Use this SOP for creating program links in your HPLC Pump K 1800 You can create links between existing programs as follows 1 Enter desired number in field Edit Prog and press gt for confirmation Press any numeric key for a line number Use gt to move the cursor to field P and choose the desired program number 4 Press gt again to select field R Enter number of runs to be performed for the selected program boc aapt Fig 17 SOP 6 Select field Wait and enter values 1 or 0 Ifwait isset to 1 the pump waits for an external start signal or an user pressing 1 before this line is executed On waiting the pump switches to wait status If Wait Is set to O the program lines of the link are processed continuously Example for a program link
34. s to the HPLC Pump K 1800 Firmware Revision 1 0X Use this SOP for choosing a solvent composition 1 Select GRADIENT MODE in the SETUP Menu 2 Select the desired mode of operation 3 If you select either high or low pressure gradient i e HPG or LPG you need to specify the channels of used solvents Low Pressure Gradients You can choose valves A to D High Pressure Gradients You can choose pump names A to D Fig 12 on page 19 gives a detailed example for gradient mode selection PUMP HEAD You can select the type of pump head installed in your pump Selection of any pump head sets specific flow rate calculating data as well as maximum system pressures with respect to its specifi cations The according maximum pressure applies to all modes of operation and can never be exceeded PRESSURE AUTOZERO You can choose an offset correction for the pressure display Make sure that purge valve is open Select menu with gt and start autozero routine by pressing A or V for auto matic correction FLOW You can enter a correction constant for the selected flow rate in the range 4000 6000 This correction changes the number of pump cycles at a selected flow rate This option allows for balancing different compressibility of solvents Only trained users should change that setting Select menu with gt position cursor on desired numeric field and adjust values with A or Y You have no access to the correction parameters see Fig 8
35. ss the corresponding arrow keys Functions of arrow keys are defined straightforward Up arrow A Left arrow lt 1 gt Right arrow V Down arrow Setup of the HPLC Pump K 1800 Unpacking Unpack the instrument and check pump and accessories for any damage due to shipping Place any claims referring to damage at the transportation company responsible for shipping Please check that the HPLC Pump K 1800 is complete use section Packing List Should in spite of our thorough control procedures something be missing please contact our service department see chapter Service Department and Hotline on page 43 Packing List Pump including 1 Power supply cable 1 User manual 1 Set Tools 1 RS232 cable 1 Set Plug strips with connectors 1 Glass syringe with connectors for piston backflushing 1 Pump head 250ml or 1000mi Please fill out the registration card for guarantee and return it imme diately Please remove the transparent protective foil from the pump outlet and the display For the complete list of soare parts please refer to chapter List of Spare Parts and Accessories on page 41 Identifying installed Pump Heads Maximum flow rate 250 or 1000 ml min isindicated on a label on the front of the pump head Setup of the HPLC Pump K 1800 6 T il Front View of the HPLC Pump K 1800 Fig 1 shows the front view of the HPLC Pump K 1800 Front Panel Elements Display information Foil key area 0 0 Pum
36. then A to scroll through the gradient mode options until LPG appears Use gt to position cursor in the second line Select ON or off at positions A B C and D for the solvent channels in either mode you wish to use The number of ONS selected refers to all programs in the pumps memory At any position you can use A or V to select on Position cursor on next position e g B by pressing Select three times gt to position cursor at the rhombus The display should appear as shown in Fig 12 on page 19 Leave this menu page with lt 1 to return to the main menu Load Edit Prog Flow Pressure Events Prog ClearO ml min 0 1 MPa oft0 oni J2 Hold 0 Time oA Y B C D Run 1 min Example for setting up gradient modes with two solvent channels in all pro grams Using the High Pressure Gradient Mode If you want to control up to three additional HPLC Pumps K 1800 for a high pressure gradient you can connect the appropriate number of pumps using the serial RS 232 interfaces according to Using the RS 232 Serial Interfaces on page 34 Activate GRADIENT MODE in the SETUP menu see Using the SETUP Menu on page 13 and select individual unique names from A to D for all pumps connected Each pump can take control over all pumps connected if execution of a program is launched on that pump Control of the HPLC Pump K 1800 20 91 SOP SOP SOP biri aapt Direct Control of the Pump If you want
37. ting the pump without solvents will lead to damage of the pump heads Features of the User Interface of the HPLC Pump K 1800 Appearance of the Cursor You can use the yellow arrow keys on the foil keypad for positioning the blinking cursor at any position of the menu screen The cursor appears as underscore character while you can enter data see Figure 5 Use Key pad for data input Pos in Fig 2 and e Blinking Cursor e Cursor waiting for next data input refer to SOP Entering Data on page 12 Menu structure of the HPLC Pump K 1800 The display of the HPLC Pump K 1800 uses one main screen with status information of the instrument From the main screen you can access four additional screens for display of parameters By default the HPLC Pump K 1800 displays the parameters of the first program line of the currently active program The center part of Figure 6 Menu structure of the HPLC Pump K 1800 shows an example of the main screen Main Screen Operation Screen LINK Men i w V load EdtProg Pos Presume Biens ys Link lt 01 100 0 000 00000000 D setup y gt view lt lt OFF 000 00 gt GLP Hold 0 Time A B C D VIEW Menu Run 1 min GLP Menu Fig 6 Menu structure of the HPLC Pump K 1800 SOP Selecting Menus This SOP applies to the HPLC Pump K 1800 Firmware Revision 1 0X Use this SOP for selecting menus in your HPLC Pump K 1800 You can access the four menus of the HPLC Pump
38. x3 1x2 connections A1402 including lever catch Set of signal cables A1404 Spare parts Refer to Figure 21 Explosion Diagram and list of parts of 250ml and 1000ml pump heads 250 ml Head Check valve Pos A1080 Piston rod 3 8 Pos A1017 Set of piston seals for piston rod with O Rings A1046 2x 3 11 1x 1000 mi Head Piston rod 3 4 A1015 Set of piston seals for piston rod with O Rings A1077 Declaration of Conformity 42 sal i birri Lap Declaration of Conformity M anufacturer s Name Wissenschaftliche Geratebau Dr Ing Herbert Knauer GmbH M anufacturer s Adress Hegauer Weg 38 14163 Berlin Germany declares under sole responsibility that the following product HPLC Pump K 1800 complies with the following requirements and product specifications e Low Voltage Ordinance 73 23 EWG EN 61010 1 1993 e Engineering Guidelines 89 392 EWG e EMV Ordinance 89 336 EWG EN 50081 1 1992 EN 55011 1991 Class B EN 55022 1987 Class B EN 50082 1 1992 IEC 801 2 1984 includes IEC 41 B sec 81 1992 IEC 801 3 1984 IEC 801 4 1988 The product was tested in a typical configuration Berlin November 11 1998 is Mhytiodt i Bernward Rittgerodt M anaging Director The CE Shield is attached to the rear of the instrument Guarantee Statement We guarantee the HPLC Pump K 1800 for a period of 12 months beginning from the date of dispatch from Berlin Operation inconsis tent wit
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