User Manual SuperCycler
Contents
1. User Manual SuperCycler Trinity Revision 1 0 2011 ak Kyratec Australia Kyratec Pty Ltd 3 17 Dividend St Mansfield Brisbane Queensland Australia 4122 Website www kyratec com AAA s amp lt Contents 26 on p a to sto dd a 4 R RTE 4 3 Getting Started E Cs Selection K E E A A ld di a 6 h 7 ETT N T 16 El User ACCOUN era heroina Detrol rendida Drs a Apudivia da lOs ST 17 Cea T 21 E MAG AOS TTT 22 O Temor AAA TTT TTT 23 Kyratec SuperCycler Trinity User Manual v1 0 2 a 1 Foreword Congratulations on purchasing a Kyratec SuperCycler Trinity You are now one of the growing number of highly skilled customers worldwide to adopt the SuperCycler Trinity as their preferred Thermal cycling Engine Our philosophy has been to generate the user friendly yet powerful cycling workhorse We trust you will be most pleased with our product however always welcome any suggestions you may have to make our products better for the future Feel free to email us support kyratec com Kyratec SuperCycler Trinity User Manual v1 0 3 D PS 2 Features 2 1 General The SuperCycler Trinity is a high performance block cycling system configured and optimised for Industry standard 200ul individual or strip tubes domed or flat capped or 96 well plates low or high skirt with strip caps or adhesive film seals It incorporates state of the art electro2. 3 1 Region Selection When the unit is powered on for first time ever the region selection and End User Licence Agreement EULA will be displayed The operator is required to select their correct region of purchase and operation read the terms of use and check the agree box then press the green tick to continue Note that available features vary between different regions The features that are available for your use are always as specified to you before your purchase of the equipment e for this equipment and agree to the tern IMPORTANT THIS END USER LICENCE AGREEMENT or EULA IS A LEGAL AGREEMENT BETWEEN THE PERSON COMPANY OR ORGANIZATION THAT HAS PURCHASED THIS EQUIPMENT YOU OR CUSTOMER AND KYRATEC BY USING THIS DEVICE CUSTOMER AGREES TO THE TERMS OF THIS AGREEMENT IF YOU DO NOT AGREE TO BE BOUND BY THESE TERMS OR DO NOT HAWE AUTHORITY TO BIND CUSTOMER TO THESE TERMS THEN DO NOT CONTINUE AND RETURN THE DEVICE TO YOUR PLACE OF PURCHASE FOR A REFUND IN ACCORDANCE WITH ITS REFUND POLICIES 1 Definitions a Supplier means Kyratec or one of its distributors b Device means Kyratec SuperCycler SC300 Thermal Cycler c Region means the country group of countries or area in which device is located d Features means software or hardware operational functionality 2 License Grants Customer is only granted rights to use the device features which are offered to them in the region in which device
3. amp cool between denat amp anneal block 96 Well block supporting 0 2 mL tubes or strip tubes with flat or domed caps 96 well high or low skirt plates with strip caps adhesive cover or oil overlay Programmable 0 24 C across block width 12 wells Automatic utilising applied pressure heated lid Controllable 60 C 115 C W 180 mm 7 D 285 mm 11 2 350 mm 13 8 including cables H 190 7 5 mm lid closed 340 mm 13 4 lid open 5 5kg 12 1 lbs Pewter on black 100 240 VAC 4 Amp 50 60 Hz Automatic voltage sense Standard IEC Inlet plug USB interface to Windows XP Vista based PC USB host port for memory stick mouse keyboard etc Embedded graphical controller with 7 widescreen touch sensitive colour backlit display Supplied with unlimited user license Free upgrades available via web download 256MB enough for 10 000 saved profiles Multiple thermal zones Touch Down Up Long Range Thermal Gradient Program Pauses Temperature Graphing On screen Help User Accounts Profile Load and Saving Manual Mode USB File Transfer Post Run Reporting Auto restart and more Power Cable User manual Touch Screen Stylus Check with your local distributor Kyratec SuperCycler Trinity User Manual v1 0 22 6 a 10 Terms of Use END USER LICENCE AGREEMENT Kyratec SuperCycler SC300 IMPORTANT THIS END USER LICENCE AGREEMENT or EULA IS A LEGAL AGREEMENT BETWEEN THE PER
4. frre ware Update Touct calibration 4 Y Done The configuration page provides access to the following features FILE MANAGER For managing the PCR run files on the device and copying from the device using a USB memory stick MACHINE SETTINGS Allows changes to be made to the instruments settings TIME amp DATE Used to set the Time for the instruments internal clock THERMAL CALIBRATION The thermal calibration feature is used to adjust the machine calibration SuperCycler Trinitys are factory calibrated and should not require user recalibration for the life of the machine The recalibration procedure is not covered in this user manual If it is required contact the distributor the equipment was purchased through for SC Service note Thermal recalibration The thermal calibration is password protected to prevent it accidentally being changed USER ACCOUNTS User account control SOFTWARE UPDATE Allows upgrading of the SuperCycler Trinity operating Software TOUCH PANEL CALIBRATION You can also perform a calibration of the touch screen interface using the calibration wizard If the calibration has varied click on this button and follow the instructions to re calibrate the screen If you are having difficulties navigating to this option because the touch screen is out of calibration then you may use a USB mouse plugged into the front USB port to reach the touch screen calibration wizard Kyratec SuperCycler Tr
5. User Manual v1 0 Solitaire Y Done 20 DD Cad 8 User Tips It is recommended before you use the device for the first time with biological samples that you experiment with setting up runs and trialling these to familiarise yourself with the machine to ensure correct operation on your valuable samples To achieve the maximum lifespan and best clarity from you SuperCycler Trinity touch screen please only use the stylus provided or equivalent or your fingers for selecting on the touch screen Using a ball point pen pencil or anything sharp will cause the screen to scratch To maximise the lifespan from the peltier devices which effect the heating and cooling there are a number of actions the user may take Do not run the unit with no tubes in the plate and the lid closed When the lid is closed and no tubes are present the top heater rests close to the 96 well block and will place high thermal load on the peltier devices Extended operation in this situation will shorten the lifespan of the peltier devices Attempt to minimise the time holding the samples at below ambient temperatures longer than required It is recommended against using the device as a refrigerator to hold samples at 4 degrees C temperature for long periods of time if you wish to maximise the lifespan of your instrument The heated lid applies substantial pressure to the tubes to ensure they are well seated into the thermal block and prevent the caps or sealing
6. film from popping off with the increased vapour pressure inside the tube When using only a single or small number of individual tubes then it is advised you place an empty tube in each of the 4 corners of the block to help absorb the pressure This will help prevent budget or thin wall plastic ware from deforming under the high temperature and pressures The Top Heater evaporation control default is 105deg C This is suitable for most applications If you find that you are getting excess condensation forming at the top of your tubes you can try increasing this temperature to 110deg C or higher Kyratec SuperCycler Trinity User Manual v1 0 21 2 Cad 9 Specifications Thermal Cycling Technology Temperature Range Temperature Accuracy Temperature Uniformity Temperature Resolution Heating Cooling Rate Well Configuration Linear thermal gradient Available selected regions only Condensation control Heated Lid Temperature Range Dimensions Weight Colour Electrical External Interface Internal Interface Software Internal Memory Functionality Included Accessories Warranty High performance active heating and cooling using quality elements x 8 amp precision sensors x 4 4 C 99 C 0 5 C over full range typically lt 0 1 C at 60 C 0 3 C 30 seconds after target range 40 C 90 C Typically lt 0 1 C at 60 C 0 1 C increments 7 C sec Peak block 5 C sec typical heat
7. use these features The following picture displays the same screen with all options Triple Zone Touch Down amp Long Range enabled Add Step 1 of 2 Set time and temperature Hold Time Initial s Hold Time Final s Temp Initial Left c Temp Final Left c Temp Initial Center c Temp Initial Right c Temp Final Right c B Triple Zone B Touchdown gten 8 long Range navio time The Next button will proceed through each of the parameter screens for each step contained within the cycling After the parameters for each step are configured the operator is prompted to enter the number of times to Kyratec SuperCycler Trinity User Manual v1 0 12 a P LA repeat the cycle Note Gradient function and Triple Zone not available in all regions or in all models 5 2 Starting a run Activating the Start Run button will show a profile review page which allows the operator to check over the profile displayed in text format before confirming commencement The profile review will also contain general run options regarding the Top Heater evaporation control and actions on run completion These are configurable under the configuration menu You have the option to perform the run in virtual mode this will simulate the run without actually thermally varying the block Once the profile is confirmed If the Use Top Heater checkbox option is selected default under the setup menu then you will be prompted with a screen asking
8. was purchased and resides Customer warrants that the selected region is the true region of its purchase and physical operational location Incorrect selection of region is in breach of supplier terms of license and may or may not breach any 3rd party license terms Customer accepts sole responsibility for incorrect region selection and consequences of any breach of 3rd party intellectual property associated with said features E agree to Terms of Use for th If the instrument is moved to a different region of operation at a later date then the region configuration screen may be reached through the settings icon under the configuration menu A copy of the Terms of use may also be found at the end of this manual Kyratec SuperCycler Trinity User Manual v1 0 6 3 2 Navigating the Software After power up the unit defaults to the main screen from where all features may be accessed SSIS Manual Control gt Configuration Y Faea Information The 4 screen icons provide access the following features CONFIGURATION Takes you to the configuration page allowing of machine options PCR RUN Is used to load or create a new PCR thermal profile and initiate its execution MANUAL CONTROL Allows immediate manual setting of block temperature INFORMATION Displays a general information screen which includes such information as serial number software and hardware version numbers An on screen user manual is accessible from un
9. B Accelerated Thermal Equilibration gt B Auto Resume Run After Power Outage gt E Rate limit heating cooling rates Max deg per sec Top Heater Settings Use Top Heater Top heater temperature deg C 105 Disable Top Heater when set temperature lt The following features are available under this page 96 well thermal block settings Audible alarm on run completion toggles whether there is an audible alarm or just a screen message to alert the operator that the run has completed Use Predicted Temperature for Hold times The SC software implements a thermal model which predicts the actual liquid temperature inside the reaction vessels Hold times can be based on this model default or based purely on the block temperature Using the predicted temperature effectively increases the hold times and total run time slightly but gives a more accurate representation of the hold time of the actual sample liquid Most manufacturers thermal cyclers purely use the block temperatures for timing Accelerated Thermal Equilibration If this feature is selected the temperature model will use overshoot and undershoot to get the liquid to temperature faster This is useful for highly sensitive chemistries or thermal profiles with short hold times Auto Resume Run After Power Outage If this feature is available on your cycler not available on all models then when this box is checked a run will automatically r
10. ES You may choose from 4 event types HOLD Is a simple hold at given temperature for a set time It may be performed either with or without a gradient across the block The hold screen allows the operator to set the hold time and temperature Depending on the machine model the option for Triple Zone or Gradient may be available If the Triple Zone option is selected then all 3 temperature Kyratec SuperCycler Trinity User Manual v1 0 11 a PA zones will become available to set independently If the Gradient option is selected both the left and right temperatures may be set separately and the predicted well temperatures can be shown by clicking the Predicted Temps link RAMP Allows the block temperature to be ramped either up or down between two set temperatures over a preset length of time The block target temperature is updated every second and with a 0 025 degree minimum step resolution It may be performed either with or without a gradient across the block The ramp screen shows the initial and final ramp temperatures Depending on the machine model the option for Triple Zone or Gradient may be available If the Triple Zone option is selected then all 3 temperature zones will become available to set independently If the Gradient option is selected both the left and right temperatures may be set separately and the predicted well temperatures can be shown by clicking the Predicted Temps link PAUSE will hold the temper
11. SON COMPANY OR ORGANIZATION THAT HAS PURCHASED THIS EQUIPMENT YOU OR CUSTOMER AND KYRATEC BY USING THIS DEVICE CUSTOMER AGREES TO THE TERMS OF THIS AGREEMENT IF YOU DO NOT AGREE TO BE BOUND BY THESE TERMS OR DO NOT HAVE AUTHORITY TO BIND CUSTOMER TO THESE TERMS THEN DO NOT CONTINUE AND RETURN THE DEVICE TO YOUR PLACE OF PURCHASE FOR A REFUND IN ACCORDANCE WITH ITS REFUND POLICIES 1 Definitions a Supplier means Kyratec or one of its distributors b Device means Kyratec SuperCycler SC300 Thermal Cycler c Region means the country group of countries or area in which device is located d Features means software or hardware operational functionality 2 License Grants Customer is only granted rights to use the device features which are offered to them in the region in which device was purchased and resides Customer warrants that the selected region is the true region of its purchase and physical operational location Incorrect selection of region is in breach of supplier terms of license and may or may not breach any 3rd party license terms Customer accepts sole responsibility for incorrect region selection and consequences of any breach of 3rd party intellectual property associated with said features 3 Limitation of Liability Use of device and any results or information generated by device are at the sole risk of customer Supplier makes no guarantees that expected functions are performed correctly
12. Supplier strongly recommends that customer perform full manner of internal controls within their reaction volumes to satisfy themselves that expected functions were performed correctly Supplier s sole responsibility is for repair or replacement of defective device within the warranty period Maximum liability of supplier in any circumstance is limited to the amount paid by customer to the supplier for the device Kyratec SuperCycler Trinity User Manual v1 0 23
13. annealing temperature during initial cycle repeats of an amplification profile Kyratec SuperCycler Trinity User Manual v1 0 4 2 LA 2 8 Pause or Infinite Holds The Pause function allows the user to pause the profile at any number of pre programmed points with an audible beep The current set temperature is held indefinitely until the Continue button is pressed Holds are useful if there is a need to remove tubes or add reagents at a particular point in a run 2 9 Auto Restart The SuperCycler Trinity is able to restart a run if power has been lost for any reason during cycling Once power is restored the run will be resumed from the last step taken Care should be taken with the reaction results as the power may have been off for a considerable amount of time 2 10 Rate Limiting The SuperCycler Trinity is able to limit the rate of heating and cooling during a run This is controllable between 0 1 C sec and 2 5 C sec This is useful for chemistry that has only been verified at the lower ramp This feature is not available in manual mode 2 11 Manual Control The SuperCycler Trinity also supports Manual Mode control This mode enables the user to set the block to a specific temperature quickly without creating a thermal profile This function is useful for incubating reactions such as DNA digestion or ligation Manual Mode also supports thermal gradient and triple zone depending on the unit purchased 2 12 Evaporation control He
14. as a powerful thermal profile engine implemented within A profile may contain up to 100 events Each event can be either a hold at temperature pause or 2 to 5 step cycling with up to 100 repeats Any event or step can contain triple zone temperatures gradient touchdown or long range features An almost unlimited number of profiles may be stored on the device for re running Despite its capabilities profile setup is straightforward 2 6 Long Range The Long Range feature enables the time of a particular cycling step to be automatically increased or decreased by a preset amount over a specified range of cycle repeats The Long Range increasing time mode feature is often used to provide gradually extended times for enzymatic polymerization of longer products during later cycle repeats of an amplification reaction It is most useful for targets longer than about 1kb Long Range decreasing time mode can be used to shorten the total run time During later cycles the majority of enzymatic polymerization is occurring on previously generated amplicons which are shorter in length than the original template Shorter hold times are often sufficient for polymerization of these shorter length products 2 7 Touch Down The Touchdown function enables the temperature of a step to be automatically decreased by a preset amount over a range of successive cycle repeats Its primary use is as a mechanism to minimise primerdimer artifacts by gradually decreasing the
15. ated Lid The SuperCycler Trinity employs a heated lid design to keep the air contained within the tube hotter than the reaction volume This causes any evaporation to condense back into the cooler reaction liquid thereby eliminating the need for oil or wax condensation overlay 2 13 User Interface The SuperCycler Trinity has a 7 colour touch screen giving a vibrant and flexible means of run setup and monitoring This internal graphical user interface controller provides a powerful yet intuitive and easy to use interface All SuperCycler Trinitys have a USB port and soon to be released Windows based PC software This software opens the user up to easier profile setup sharing of files and post run examination of any temperature data New software features and updates are regularly incorporated and available for either system as free upgrades via web download Kyratec SuperCycler Trinity User Manual v1 0 5 4 PA 3 Getting Started Plug the unit into a power outlet and power the unit on with the switch located on the rear of the unit Do not worry about selecting the correct power voltage for your country The SuperCycler Trinity is designed to operate in any country in the world with no changes The unit is also designed to be resistant to power surges and drop outs that occur in countries with less reliable power grids On power up the SuperCycler Trinity will display the logo and take a few moments to initiate the software
16. ature at the last set point and pause the run with an audible warning This is useful if the operator needs to perform a certain action of the samples at a given point in the run The operator clicks ok when complete and the run will continue with the next event CYCLING x STEP will proceed to generate a multi step cycling event The scroll button to the right allows you to change the number of steps between 2 and 5 for the cycling event Within the cycling configuration screens there are numerous options Depending on the machine model the option for Triple Zone or Gradient may be available If the Triple Zone option is selected then all 3 temperature zones will become available to set independently If the Gradient option is selected both the left and right temperatures may be set separately and the predicted well temperatures can be shown by clicking the Predicted Temps link There are also options for Touchdown and Long range features Selecting these checkboxes will display extra buttons to configure these features Touch Down allows for the temperature of a given step within a cycling event to be progressively increased or decreased from an initial value to a final value over a defined number of steps Long Range allows the hold time of a given step within a cycling event to be progressively increased or decreased from an initial value to a final value over a defined number of steps Any or all of the steps within a cycling event may
17. der this page menu Throughout the software help is available in numerous locations by pressing the question mark logo if available Kyratec SuperCycler Trinity User Manual v1 0 7 4 PCR Profile Creation From the home screen the PCR Run icon will bring you to the experiment page Here you will have 4 options and you can load an existing experiment from either the internal drive or an external USB drive or create a new thermal profile using either the Wizard or the Manual Experiment Editor Load Existing Experiment New Experiment ear 8 Internal USB Drive Wizard Manual Drive Experiment Editor When loading files from the Internal Drive you only have access to the files under the directory of the user that you are currently logged in as If you are not logged in as a registered user your file access will be under the Default user directory 4 1 Wizard Mode The SuperCycler Trinity software includes a Wizard utility which enables the user to configure easy to moderate complexity profiles in just moments To activate select Wizard from the PCR start up screen PCR Experiment Wizard m cDNA Preheat Activation Denaturation Temperature c Anneal W Triple Zone Extension step Post Cycling Extension Hold on Completion Use Top Heater deg c 105 Kyratec SuperCycler Trinity User Manual v1 0 8 2 E A The Wizard screen displays each of the steps which occur in a typical range of profi
18. ebsite should be placed on the main root directory of your USB memory stick You may then select it to upgrade the unit s software After selecting the software file read the message box and press OK and wait Upgrade will take up to 1 2 minutes Once upgrade is complete you can check that the new version is installed by visiting the Information page from the home screen and check the installed software version IMPORTANT NOTE Do not interrupt the unit while a file upgrade is in progress Switching off the power during upgrade may render the unit inoperable After upgrading software the internal on screen user manual may need to also be updated Click on one of the help icons it will display an upgrade message if a user manual update is also required To update it you need to download the correct user manual zip file from our website and unzip it on to the root main directory on a USB memory stick and then insert it into the SuperCycler Trinity and follow the upgrade prompts when you try to access the out of date help system 7 5 Games amp Extras Under the extras tab you will find some other programs The Oligo Calculator is useful for calculating the theoretical melt temperature and other information pertaining to the oligo design Simply enter the sequence and it does the rest Several popular Games are also provided for your enjoyment Kyratec SuperCycler Trinity User Manual v1 0 19 Oligo Calculator Kyratec SuperCycler Trinity
19. esume at the same place if power should be momentarily lost during a run Rate limit heating cooling rates sets the rate limit for a run This has a limit of 0 1 C sec to 2 5 C sec This feature is only available in runs not manual mode Top heater evaporation control settings Use Top Heater will cause the top heater to be used during a run if it is checked Entry box for top heater temperature Temperature is programmable between 30 110 C default 105 Button for manually enabling the top heater to preheat it if required Disable top heater when set temperature lt 30deg feature is implemented to reduce the high thermal load placed on the peltiers when holding the samples at low temperatures i e 4 degrees This prevents excess heat from the top heater heating the chilled samples It is best to leave this option enabled Note that the top heater is only activated when a run is started or the operator enables it in manual mode Kyratec SuperCycler Trinity User Manual v1 0 18 PA The Region Configuration button enables the Operational region to be changed should the instrument be moved to a different country Note that available features vary between different regions See Section 2 1 for more information 7 4 Software Update Upgrade Software A facility has been provided to update the software which resides on the SuperCycler Trinity The upgrade file you receive from our support team or the w
20. if you wish to wait for the top heater to reach target temperature before commencing the run If you select to wait for the top heater to achieve temperature a waiting screen will be displayed You may skip the waiting step at any time if you wish to commence the run immediately Depending on the set temperature of the Top Heater it will take up to several minutes to pre heat to this temperature Once the run commences the software will automatically proceed to the Run screen You are not able to modify the profile once a run has commenced b ___ o_o 2 gt E5 gt UUU ADORA 80 60 40 20 Run Controls Kyratec SuperCycler Trinity User Manual v1 0 13 On run completion the post run report screen will show and if enabled an audible alarm will sound The alarm may be muted by pressing the mute alarm button The post run report can be saved as a text file to a USB memory stick for inclusion into lab books etc Note that post run reports are not saved to internal memory Post Run Report e run report to 3 stick Kyratec SuperCycler Trinity User Manual v1 0 14 6 Manual Block Control 80 60 40 20 Run Controls Temp Left mnn E Triple Zone L E Top heat kadin pa E Timed Hold 9 The manual mode screen allows immediate manual setting of the block temperature To activate this mode simply press the blue start button Terminating the mode is achieved by pres
21. inity User Manual v1 0 16 7 1 User Accounts at Jenny James T Andrew S Add New User Kim Masters x Select User A x Cancel Delete User In the users area you are able to select an existing user or create or delete a user profile When a user is selected thermal profiles will be loaded or saved to a directory specific to that user Logging out will change back to default user To create a new user select the Add New User icon and then enter the user name and choose a user profile image 7 2 File Manager File Manager SuperCycler USB Drive E to USB F Home A Done The file manager area enables you to transfer PCR run files between the default or individual user directories and a USB memory drive Plug your USB drive into the connector on the front of the unit and you may browse the directories on the SuperCycler Trinity and transfer files to or from You may also delete any thermal profiles from the SC Press on the Home icons to refresh the directory list back to the root directories Select files in either the SC or the USB stick and press the transfer logos to copy the files across Files may be moved between different user directories by first copying them to a USB stick and then back into the destination user directory Kyratec SuperCycler Trinity User Manual v1 0 17 7 2 Machine Settings Block Settings Audible alarm on run completion Use predicted liquid temperature for hold times gt R
22. les At a minimum there are 3 steps Preheat and then denaturation and anneal which are cycled You may enable up to a further 3 steps cDNA Extension amp post run hold by clicking on the corresponding check boxes Each of the steps will be executed in the order they are shown arranged down the screen with the Denaturation Anneal and Extension if enabled steps being cycled After the amplification run has been completed the temperature may be held at a defined temperature until the user disables the unit To adjust any of the parameters click on the corresponding time or temperature boxes and a keypad will be presented allowing entering of new numbers The number of cycles can also be adjusted between 1 single execution no loops and 99 cycles Confirm Run Experiment containing 2 events Top Heater Enabled 105 ded C Maximum Heat Cool Speed Event 1 Hold Temp 95 Hold Time 0 10 00 Event 2 Cycling 3 steps 35 repeats step 1 Temp 95 Time 0 00 30 step 2 Temp 60 Time 0 00 30 step 3 Temp 72 Time 0 00 30 Audiable alarm will sound on completion End Experiment E Run in virtual simulated mode When the user is happy with the profile parameters just click on the tick A run review screen will now be shown with a text description of the profile steps Click on OK to commence run execution or Edit Profile if you require to further modify the wizard created profile The Save amp Run Icon al
23. lows you to save the profile then run it Once the profile is confirmed if the Use Top Heater checkbox option is selected default then you will be prompted with a screen asking if you wish to wait for the top heater to reach target temperature before commencing the run If you select to wait for the top heater to achieve temperature a waiting screen will be displayed You may skip the waiting step at any time if you wish to commence the run immediately Depending on the set temperature of the Top Heater it will take up to several minutes to pre heat to this temperature When the run commences the software will automatically proceed to the Run screen You are not able to modify the profile once a run has commenced but you may save or edit it after it completes or is terminated From the run review screen you may also check the option run in virtual mode This will make the SC simulate the run rather than performing the actual thermal steps on the block This can be useful if you wish to check complicated runs prior to performing them Kyratec SuperCycler Trinity User Manual v1 0 9 a PA 5 Manual Profile Generation and Execution The manual profile editor is evoked from the PCR Experiment screen This screen displays the current experiment profile in a tree list format The run will be displayed graphically on the right side of the screen showing a maximum of five steps at a time Arrows on the graph allow navigation of the step
24. nics precision quality Ferrotec peltier devices and a flexible user interface accessed via a vivid touchscreen display 2 2 Configuration The SuperCycler Trinity utilises six Peltier devices to actively heat and cool the block between 4 C and 99 C These are arranged into three zones with independent sensing and control capabilities 2 3 Triple Zone The unique Trinity Triple Zone block format allows you to not only optimize your reactions but to also run multiple differently thermal optimised reactions in a single run at exactly defined temperatures Whilst a gradient instrument is useful for optimising a given reaction it does not generally provide as effective a means of running more than one optimised reaction at a defined independent temperature The SuperCycler Trinity can run reactions with up to a 20 C difference zone to zone 2 4 Gradient available in selected regions only Thermal gradient technology enables a varying temperature to be set across the reaction wells of the block Applications of this feature include the ability to optimize the annealing temperature of an assay in a single experiment by determining the temperature of the wells that yield the best result The SuperCycler Trinity is capable of generating a gradient of between 0 C and 24 C across the block The software interpolates and displays the temperature of each column of wells in real time during a run 2 5 Thermal Profile Engine The SuperCycler Trinity h
25. s The event parameters can be edited numerous ways To edit the temperature or time of an event touch the corresponding temperature or time on the graph to edit them If the event has a touchdown or long range step then the entire event will be reviewed by pressing the text Selecting the event on the Tree list allowed the entire event to be edited deleted or moved up or down in order E Experiment unsaved User Default Run time 1 35 44 0 10 00 Top Heater Enabled 105 deg C Rate Limit Max Ho ld Temp 95c Time 0 10 00 Ul Cycling 35 Repeats Step 1 Temp 95c Time 0 00 30 Step 2 Temp 60c Time 0 00 30 Step 3 Temp 72c Time 0 00 30 Move Up Move Down Edit Delete The profile generation screen contains 7 icon buttons with the following functionality HELP This button will display the on screen user manual with the relevant pages selected START RUN This button is used to activate a run Pressing this button will show a profile review before commencing a run ADD EVENT Evokes the Add Step wizard to build a new profile step after the last current step SAVE Allows a given profile to be saved to internal flash memory disk The unit has very large internal memory allowing virtually unlimited profiles to be saved in the order of tens of thousands Saving a profile also stores the device options configurable under the settings menu i e top heater temperature and other func
26. sing the red stop button To Program the set temperature press the temperature button to the left of the graph and enter the desired value You may program the temperature set point to 0 1 degree C resolution Depending on the machine model the option for Triple Zone or Gradient may be available If the Triple Zone option is selected then all 3 temperature zones will become visible These temperatures refer to the left center and right zones when looking at the machine They are set independently of each other If the Gradient checkbox is checked the Temp Right button appears and you are able to set different temperatures for both the left and right sides of the block with a roughly linear thermal gradient presented between the two outer well sides To enable the Top Heater evaporation control click the Top Heat checkbox The target temperature of the top heater is configured under the Configuration gt Settings menu Note the top heater will take a few minutes to reach its operating temperature The left side of the screen displays the temperatures of each of the 4 zones Other information also displayed is the event step and cycle numbers for when a profile is being executed At the bottom of this display box is the top heater TH and internal heat sink HS temperatures Note gradient feature not available all regions Kyratec SuperCycler Trinity User Manual v1 0 15 7 Configuration Tools Time amp Date Thermal Calibration
27. tionality You may enter any valid file name for saving Profile file will be saved to the current user directory or the Default directory if no user is selected LOAD Loads a pre saved profile from the internal flash memory The SuperCycler Trinity displays a list of the saved files on the unit in the directory of the currently selected user If no user is selected it will show the files saved in the default user directory To access a different user s files you need to log in as that user Cancel Returns the user to the experiment page Kyratec SuperCycler Trinity User Manual v1 0 10 Experiment Allows the operator to edit the experiment details By selecting the user picture brings up the user log in screen By selecting the title of the experiment brings up the keyboard and allows the title to be edited The sample volume can be edited by using the up down arrows Notes can be added to the experiment by pressing the link The thermal details can be edited by pressing the link See 7 3 Machine Settings for details Experiment Details User 41 Default Experiment Title Unt Sample volume ul 25 5 1 Creating a new Event To create a new event select the Add Event icon in the manual profile editor The first screen of the Add Event Wizard which will be displayed lets the user select the type of event What kind of event would you like to add U LF 2 E Step E cycling hold melt
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