uniSPEC 2 USER MANUAL Master 2014_07_25_UK - LLG
Contents
1. Quantitative Measure Print Conc Unit Qty of Standards Conc Of Standards Display the Curve Select Curve Setup Set Absorbance Transmittance Energy Measurement Measure Print Standard Curve Measurement Set Wavelength Set Parameters Coefficient Choose Equation D2 Lamp W Lamp Time Set Get Dark Current WL Calibration Version Load Default Fig 4 1 Menu Tree 11 Measurement Set WL Measure Print Set Parameters Coefficient K Coefficient B Conc Unit 1 2 Firmware Functions The firmware consists of 3 functions Basic Mode Quantitative System Functions 1 2 1 Basic Mode Measure the Absorbance Transmittance or Energy in a fixed wavelength point The test result can be stored in the RAM 1 2 2 Quantitative Standard Curve Method Set up standard curve by standard samples use the new curve to measure the unknown sample s concentration The curves and the test results can be stored in the RAM Coefficient Method Input the values of the curve equation s coefficient2. 24 Note Remove the cuvettes from the light path before this action don t open the lid of the compartment during the course of calibration Ohan E ara Press _ to return when it finishes VSetup O W Lamp On Getting Dark O Date amp Time e Dark Current Fig 4 29 4 3 5 Wavelength Calibration After some time the energy of lights would drop off and there will be some but slight influence on the test result In this case users can reset the wavelength to compensate We suggest the users to reset the wavelength every one or two months Remove all the blocks in the light path and close the lid of the compartment press Arrow Key to choose Calibrate WL and press to begin the calibration Fig 4 30 The lid of the compartment should not be opened during the calibration It returns automatically after calibration VSetup O Date amp Time Calibrating WL Dark Current e Calibrate WL Fig 4 30 4 3 6 Lamps Change Input the lamps changing wavelength point The instrument permits the users to set the lamps changing wavelength point In the range of 300 400nm users can choose the lamp freely Oat tmnt ara a Press Arrow Key to choose Lamp Change and press to go into corresponding setting interface Fig 4 31 Input the value of lamps changing wavelength point by the numeric keypad 300 400nm then press gt Oat Curent abr NL Please pu l
3. and then measure the unknown sample s concentration 1 2 3 Setup Light source management Get Dark Current Calibrate Wavelength and Set time etc 2 Basic Operation 2 1 Choose Photometric Mode Bell emer Basic Mode Ounae FA O00 bs Osip M Mim ESC Press Arrow key to choose and to confirm 2 2 Set Wavelength In the measurement Interface press to set wavelength use numeric keyboard to input the value press to confirm and set 100 T OAbs automatically 2 3 Set Parameters O dis op a tim Press 5 to go into the parameter setting interface press Arrow Key to choose the corresponding menu and use numeric key to input the parameter ave Basic hode 0 000 Abs 50 0nm a Basic Mode 0 000 Abs 50 0nm Basic Made O Quenitave Sap 8 oe to return press to confirm press 2 4 Set the position of auto cell holder The Auto Cell Holder is optional In the measurement Interface press first then press the numeric key of 1 8 and then the corresponding cell will be positioned at the light path a12 O Baila a ania 0 Quantitative 1 000 Abs Sap S sin to delete the test result and stored In the measurement Interface press data 2 7 Calibrate 100 T 0Abs AO is oap tt Put the re
4. setting interface Fig 4 26 Press Arrow key to choose On or Off and gap bela th Asap 163 belay ODM n On Olan M OOn y ori OOF n be n Ott press to confirm Press to return VSetup D2 Lamp OW Lamp O Date Time Fig 4 26 Aa 4 3 2 Power on off W Lamp i When the wavelength point needed is in the range of 190 339nm the W lamp can be switched off to prolong its life time fp Site Use the key of Arrow Key to choose W Lamp press to go into W lamp setting interface Fig 4 27 Use the key of Arrow Key to choose m om lap ap mA Ollan On On 1 OW h Datek Tine to return On or Off and press to confirm Press Setup O D2Lamp On 9 W Lamp On O Date Time Fig 4 27 4 3 3 Set Date amp Time Press Arrow Key to choose Date Time and press eae to go into setting interface Fig 4 28 Use Arrow Key to choose setting item and input the value by numeric keypad Hour 0 23 Minute 0 59 Second 0 59 Year 0000 9999 Month 1 12 Day 1 31 Week 1 7 press to return VSetup D2 Lamp Timell 12 00 00 O W Lamp On Datel 2008 08 08 e Date amp Time Fig 4 28 4 3 4 Calibrate Dark Current i When the working condition changes A dark current calibration is required before any measurement Use Arrow key to choose Dark Current and press to confirm Fig 4 29
5. Clean the cuvettes 2 6 Incorrect reading Possible Reason Dark Current Error Worse matching of the cuvettes Solution Re get the dark current Improve the matching of the cuvettes 3 Spare part replacement Replace the Fuse AN Danger Be sure to switch off the power and unplug the socket before replacement Step 1 Tools preparation Prepare a 3x75 Flat Blade screwdriver Step 2 Switch Off the power supply Switch off the power supply and unplug the socket Step 3 Take out the Fuse Seat Take out the fuse seat by the screwdriver Fig 5 1 Spare Fuse 28 Step 4 Replace a new fuse Pick out the spare fuse and replace it to the working position Fig 5 2 Fig 5 2 Step 5 Reset the fuse seat Replace the fuse seat in the power socket Step 6 Switch on the power Plug the socket and switch on the power 29
6. If the local voltage is not stable enough a manostat is required Be away from direct sunlight 2 Voltage Confirmation Be sure to set the instrument s Voltage Switch at your local power AN supply or severe damage may occur The Voltage Switch is on the bottom of the instrument e g If your local Voltage is 220V you should set the Voltage Switch at the following status Fig 3 1 Fig 3 1 If your local Voltage is 110V you should stir the switch to the Left way by finger so that it can be set at 110V Fig 3 2 Fig 3 2 3 Installation Step 1 Check the packing list Unpack the contents check the materials with the Packing List Any damage or Lost found please contact us or the local dealer Step 2 Position Place the instrument on the stable table carefully Step 3 Install printer Optional Make sure the printer is power off link the printer s data cable to the instrument s parallel port Step 4 Link the power cord Make sure the instrument s power switch is in the Off condition link the power cord to the instrument and insert another end in the socket provided with a protective earth contact Step 5 Switch on the power Check again make sure that all the links are right Switch on the power The instrument can be used to measure after self test and pre warm 10 CHAPTER 4 OPERATION INTRODUCTION 1 Software Structure 1 1 Menu Tree Main Menu Basic Mode
7. k Move the cursor on Load Curve and press gt to confirm Then the system will go into the curve equation record interface Fig 4 17 19 Standard Curve _ 3 O Create Curve 1 852A 2 010 a aA Lal Load Curve O Delete Curve Fig 4 17 Move the cursor on the equation that you need and press Mo to confirm Then the corresponding curve will display on the screen Fig 4 18 2 C 1 000A 0 000 Fig 4 18 Press to go into sample continuous measure interface and measure samples Note When you reload the curves the wavelength will be set at the point where you create the Standard Curve automatically 7 Delete Curve 0 de 2 CER 0040000 X Move the cursor on Delete Curve and press to confirm Then the system goes into the curve equation choosing interface Fig 4 19 No Curve Data Sta ndal d Cu rve A 4 Ors A tS Na J SE ba Fe ode PA OALA 7 Som Me O Create Curve 2 C 1 000A 0 000 Load Curve Delete Curve ESE Fig 4 19 Move the cursor on the curve equation that you don t need again and press Fig 4 20 then system will ask you to confirm again 20 Delete Curve 5 Z gt n oe A molto af rc T A 0 000 O YES NO a ad 2 C 1 000 Fig 4 20 ba os ait Sik hs o Iba Ml j A Move the cursor on Yes and p
8. 7 Please Input WL to go into Parameter Setting Interface press Array Key to hse e Bache gt Lis e A i te select Abs T or Energy mode press to confirm Basado 0e schoe Lis f i 500 mm Press t to return Fig 4 4 Basic Mode 0 000 Abs O T 500 0 nm Energy 14 Step 4 Go into Continuous Measurement Interface START STOP Press to go into continuous measurement interface Fig 4 5 Basic Mode 500 0nm 0 000Abs No WL Abs 0 000 Abs 500 0 nm Fig 4 5 Step 5 Calibrate 100 T 0Abs Put the Reference Solution in the light path press to calibrate 100 T OAbs Step 6 Measure Sample START STOP Put the unknown concentration sample in the light path press to measure and the result displays in the data sheet At the same time the test result will be stored in the RAM automatically Repeat this step to finish all the samples measurement Step 7 Print the data Press to print the test result Step 8 Delete the data Press to delete the test data 4 2 Quantitative Step 1 Go into Quantitative Interface ENTER In the main menu press Arrow Key to choose Quantitative Press to enter the Quantitative method selecting Interface Fig 4 6 15 VQuantitative OBasic Mode Standard Curve AQuantitative Coefficient Step 2 Choose Unit ENTER Move the cursor on Unit pr
9. LLG LABWARE USER MANUAL SPECTROPHOTOMETER uniSPEC 2 Contents Chapter 1 Preface 1 Safety 2 General 3 Electrical 4 Warning 5 Performance 6 Radio Interference Chapter 2 Introduction of the Instrument 1 Brief Introduction of the Instrument 2 Working Principle 3 Structure 4 Unpacking Instructions 5 Specifications Chapter 3 Install Instrument 1 Environment Required 2 Voltage Confirmation 3 Installation Chapter 4 Operation Introduction 1 Software Structure 1 1 Menu Tree 1 2 Firmware Functions 1 2 1 Basic Mode 1 2 2 Quantitative 1 2 3 Setup 2 Basic Operation 2 1 Choose Photometric Mode 2 2 Set Wavelength 2 3 Set Parameters 2 4 Set the position of auto cell holder 2 5 Delete the Input Value 2 6 Delete the test result and stored data 2 7 Calibrate 100 T OAbs 2 8 Measure Samples 2 9 Print the test result 3 Preparation before test 3 1 Switch on the instrument and begin the system self test 3 2 Pre warm 3 3 Check the cuvettes CON O O O1 O A A HW WW W O 11 11 11 12 12 12 12 12 12 12 12 12 13 13 13 13 13 13 13 13 13 4 Measurement 4 1 Basic Mode 4 2 Quantitative 4 3 Setup 4 3 1 Power On Off D2 Lamp 4 3 2 Power On Off W Lamp 4 3 3 Set Date and time 4 3 4 Calibrate Dark Current 4 3 5 Wavelength Calibration 4 3 6 Input the lamps changing Wavelength Point 4 3 7 Reload Default 4 3 8 Version Chapter 5 Instrument Maintenance 1 Daily Ma
10. ODUCTION OF THE INSTRUMENT 1 Brief Introduction of the Instrument This instrument is a single beam general purpose instrument designed to meet the needs of the Conventional Laboratory This instrument is ideal for various applications such as Chemistry Biochemistry Petrochemistry Environmental Protection Food and Beverage Labs Water and Waste Water Labs and other fields of quality control and research This instrument incorporates a 128 x 64 dots matrix LCD display for photometric results easy operation and wavelength range of 190nm to 1100nm This instrument is ideal for measurements in the visible and ultraviolet wavelength region of the electromagnetic spectrum Fig 2 1 Front View CELL CLEAR PRINT Fig 2 2 Control Panel AIR INLET FUSE PARALLEL PORT FAN POWER SWITCH POWER SOCKET USB PORT Fig 2 3 Back View 2 Working Principle Different matter has different but special absorbance wavelength point Also when at the fixed wavelength point the absorbance has some relation to the substance s Always transparent Solution concentration and its thickness The relation can be concluded as the following Formula which is called Lambert Beer Law T Il A KCL log I I A Absorbance C Concentration of the Solution K Absorbance Coefficient of the Solution L The length of the Solution in the light path The intensity of the light focused on the A D after it permeates the solution to be measured The inten
11. amps Chang pill es ile 1 O confirm and return 25 Setup O Dark Current O Calibrate WL e Lamps Change 4 3 7 Load Default ap ES oe 1 Press Arrow Key to choose Load Default and press to confirm ep Voe mania Fig 4 32 press Arrow Key to choose Yes and press to confirm Then the system will recover the default setting when leave factory Choose No with pressed to cancel the operation and return Setup O Reset WL O Lamp Change e Load Default Fig 4 32 4 3 8 Version gia VA Heth 144 Press Arrow Key to choose Version and press to confirm You ll axon tial see the version of the software and hardware Fig 4 33 Press to return Setup O Lamps Change Load Default e Version Fig 4 33 Note For the reason of updating the Version you see on the instrument may be different from the one listed in Fig 4 33 26 CHAPTER 5 Instrument Maintenance To keep the instrument work in good condition constant maintain is needed 1 Daily Maintain 1 1 Check the compartment After measurement the cuvettes with sample solutions should be taken out of the compartment in time Or the volatilization of the solution would make the mirror go moldy Users must pay more attention to the corrosive sample and liquid easy to volatilize Any solution remains in the compartment should be wiped off immediately 1 2 Sur
12. brate 100 T 0Abs Coeficient ir Mha lA Mtas e ls im Press to go into continuous measurement interface Fig 4 24 Pull the Reference Solution in the light path then press to calibrate 100 T OAbs Coefficient 500 0nm 0 000Abs 0 000 Abs No Abs Mg ml 0 000 mg ml 500 0 nm Fig 4 24 5 Measurement Place the samples to be measured in the light path press oe to measure then the result will display on the screen Repeat this step to measure other samples All the test results will be listed on the screen and stored in the RAM automatically They can be reloaded even after a sudden power cut e Step 4 Print the test result In the continuous measurement interface press to print the test result Step 5 Delete the test data am Ose S PAM In the continuous measurement interface press 00 OW Ath ese to delete the test result 4 3 SETUP y ride a In the main menu press Arrow Key to choose Setup and press to go into utility setting interface Fig 4 25 OBasic Mode OQuantitative OD2Lamp On 08 08 O W Lamp On OSetup 08 16 ESC O Date amp Time Fig 4 25 4 3 1 Power on off D2 lamp When the wavelength point needed is in the range of 340 1100nm the D2 lamp can be switched off to prolong its life time ocos vuy am Wlam n Dated Tine 8 Use Arrow key to choose D2 Lamp and press to go into D2 lamp
13. e Average value of the two results as No 1 Standard Sample s Absorbance Value 500 0nm 0 100Abs 500 0nm 0 100Abs Put 2 Standard Test Std 1 2 Input 2 Conc C E Fig 4 14 fe IM Test Sid 6 y a to confirm 18 oh last sample is finished press Curve and its equation will display on the screen automatically Fig 4 15 At the same time the Curve Equation will be saved in the RAM automatically 500 0nm 0 600Abs 0 000 Test Std 6 2 a aoe r 1 000 C 1 000XA 0 000 Fig 4 15 Note If there s some mistake during the operation the system will beep 3 times and return the initial interface automatically Of course the curve can not be displayed on the screen 5 Measure Samples P f TT gl lbs Mala to am am le Pull the Reference solution in the light path and press to go into the continuous measurement interface Fig 4 16 500 0nm 0 000Abs 0 000 0 000 r 1 000 C 1 000XA 0 000 Et Fig 4 16 Pull the unknown sample in the light path then press gt to measure The result will be displayed on the screen one by one At the same time the data will be saved in the RAM automatically Total 200 groups of data can be saved 6 Load Curve All the curve equations will be saved in the RAM automatically If you want to reload the saved curve just follow the following steps initte 5 gt Odedletue Lan
14. ess to go into concentration unit choosing interface Fig 4 7 Use Arrow Key to move the cursor on the unit you want and press to confirm VQuantitative 5 5 mi O Standard Curve nal O Coefficient 7 TA Unit 8 None Step 3 Choose Methods There are two methods to be selected Standard Curve and Coefficient l Standard Curve 1 Go into Standard Curve Interface n Quantitative menu press Arrow Key to choose Standard Curve ENTER Press to go into sub menu Fig 4 8 Here you can create a new curve or you can load a saved curve If you want to delete a saved curve just choose Delete Curve VQuantitative Standard Curve Standard Curve ete O Coefficient oad Curve O Unit O Delete Curve Fig 4 8 2 Set Wavelength ENTER A Move the cursor on Create Curve the press to go into the sample quantity setting interface Fig 4 9 16 Standard Curve Create Curve O Load Curve Input Stds No O Delete Curve 3 ESC Fig 4 9 iMm Press to go into the wavelength setting interface input the uae Gm int Sth Wn Ye to confirm Fig WL Value by the numeric Keypad and press 4 10 age Quantitative Input Stds No WL 500 0nm Please Input WL w Fig 4 10 Use the numeric keypad or Arrow Key to input the quantity of the standard samples e g There are 6 Standard Samples then pres
15. face Clean The cover of the instrument is with paint Please use wet towel to wipe off the drip on the surface immediately Organic solution is forbidden to be used to clean the cover Please wipe off the dirt on the cover timely 1 3 Clean the cuvettes After every test or after a solution change the cuvettes should be cleaned carefully or the remains on the surface would cause measuring error 2 Troubleshooting 2 1 Dark Current Error when self test Possible Cause Solution Open the lid of the compartment Close the lid of the compartment during the course of self test and switch on the power again 2 2 No response after power on Possible Cause Solution Bad contact in power supply Improve the contact Fuse melt Replace a new fuse 2 3 Printer not work printer error Possible Cause Solution No power supply Switch on the power supply Bad contact in power supply Improve the contact Bad contact of the data cable Improve the contact 207 2 4 No Stable Reading Possible Cause No enough pre warm Glass cuvettes used in UV Range No stable Sample Much higher sample concentration Low voltage or unstable power supply Lights defect Light used up 2 5 Worse Repeatability Possible Reason Unstable sample Cuvettes polluted Solution Increase the pre warm time Use Silicon Cuvettes Improve the sample Dilute the sample Improve the power condition Replace a new lamp Replace a new lamp Solution Improve the sample
16. ference in the light path press 3 to calibrate 100 T OAbs 2 8 Measure the samples Put the samples in the light path and press to measure 2 9 Print the test result In the Measurement Interface press to print the result 3 Preparation before test 3 1 Switch on the instrument and begin the system self test Diagnostic Program Make sure to remove all the blocks in the light path Close the compartment lid and switch on the power Then the system will begin self test Note During the Diagnostic Program runs please DON T open the lid of the compartment 3 2 Pre warm When the diagnostic program finished it goes into pre warm condition 20 minutes is required before measure 3 3 Check the Cuvettes The cuvette should be clear and there s no solution remains on the transparent surface If the wavelength is below 340nm Silicon Cuvette is a must 13 4 Measurement 4 1 Basic Mode Step 1 Go into Basic Mode interface In the main menu use Arrow Key to choose Baisc Mode press to go into corresponding setting interface Fig 4 2 Basic Mode O Quantitative 0 000 Abs O Setup 08 08 500 0 nm 08 26 Fig 4 2 Step 2 Set Wavelength aide om Baie 1 to set Wavelength input the wavelength value by the numeric Pal Ud As e g a Keypad then press 2 to confirm Fig 4 3 Basic Mode 0 000 Abs 500 0 nm WL
17. grating Upon leaving the sample compartment the beam is passed to the silicon photodiode detector and causes the detector to produce an electrical signal that is displayed on the digital display 4 Unpacking Instructions Carefully unpack the contents and check the materials against the following packing list to ensure that you have received everything in good condition Packing List Description Quantity POCO ONO IO ME ramales 1 Ss A O wagcnueee 1 Glass COVE TOS ctas 1 Set of 4 Quartz CUVE 8S oooccccccocccccconononononnananana nono nonannna nana nananannns 1 Set of 2 ODE ION IMAN ra orita 1 Note The Printer and Auto cell Holder mentioned in this manual are all optional accessories they do not come standard with the instrument 5 Specifications CHAPTER 3 INSTALL INSTRUMENT 1 Environment Required To ensure the best performance the following conditions are required The best working temperature range is 16 30 C and the humidity is 45 80 Keep it as far as possible away from the strong magnetic or electrical fields or any electrical device that may generate high frequency fields Set the unit up in an area that is free of dust corrosive gases and strong vibrations Remove any obstructions or materials that could hinder the flow of air under and around the instrument The power requirement is 220 22V 50 1Hz or 110411 V 60 1 Hz Use the appropriate power cord and plug into a grounded outlet
18. intenance 2 Trouble shooting 3 Spare part Replacement 14 14 15 23 23 24 24 24 20 20 26 26 27 21 21 28 CHAPTER 1 PREFACE 1 Safety The safety statements in this manual comply with the requirements of the HEALTH AND SAFETY AT WORK ACT 1974 Read the following before installing and using the instrument and its accessories This instrument should be operated by appropriate laboratory technicians 2 General The apparatus described in this manual is designed to be used by properly trained personnel in a suitable equipped laboratory For the correct and safe use of this apparatus it is essential that laboratory personnel follow generally accepted safe procedures in addition to the safety precautions called for in this manual The covers on this instrument may be removed for servicing However the inside of the power supply unit is a hazardous area and its cover should not be removed under any circumstances There are no serviceable components inside this power supply unit For this instrument avoid touching the high voltage power supply at all times Some of the chemicals used in spectrophotometry are corrosive and or inflammable and samples may be radioactive toxic or potentially infective Care should be taken to follow the normal laboratory procedures for handling chemicals and samples 3 Electrical Before switching on the apparatus make sure it is set to the voltage of the local power supply see Installati
19. on The power cord shall be inserted in a socket provided with a protective earth contact The protective action must not be negated by the use of an extension cord without a protective conductor 4 Warning Any interruption of the protective conductor inside or outside the apparatus or disconnection of the protective earth terminal is likely to make the apparatus dangerous Intentional interruption is prohibited Whenever it is likely that the protection has been impaired the apparatus shall be made inoperative and be secured against any unintended operation Note NEVER touch or handle the power supply on this instrument due to the high voltage The protection is likely to be impaired if for example the apparatus A Shows visible damage A Fails to perform the intended measurements A Has been subjected to prolonged storage under unfavorable conditions A Has been subjected to severe transport stresses 5 Performance To ensure that the instrument is working within its specification especially when making measurements of an important nature carry out performance checks with particular reference to wavelength and absorbance accuracy Performance checks are detailed in this manual 6 Radio Interference For compliance with the EMC standards referred to in the EC Declaration of Conformity it is necessary that only shielded cables supplied by us are used when connecting the instrument to computers and accessories CHAPTER 2 INTR
20. ress to confirm then the curve equation will be deleted If you don t want to delete it just dale m Cea la tek addy Detit f choose NO or press Ms atk Mogh oi a e to return Il Coefficient Method 1 Go into Coefficient method iat Mn Press Arrow Key to choose Coefficient press babas 5 Cuba Mm Ms ibit In dad Ma 3 to go into biit F tai it lp LN Jat de Dh Bkt hy il pre test interface then press to go into the parameter setting interface Fig 4 21 Quantitative Coefficient O Standard Curve 0 000 Abs Coefficient K Coefficient 0 000 mg ml O Coefficient B O Unit 500 0nm Fig 4 21 2 Parameter Setting A Use Arrow Key to move the cursor on Coefficient K and press 5 to go into K setting interface Fig 4 22 Input K and press to confirm the system will return the former interface automatically Coefficient e Coefficient K O Coefficient B ais X Input the value of B in the same way press to go back to the pre test interface 3 Set Wavelength Press to go into wavelength setting interface input the ql i wavelength value by the numeric keypad and press to confirm Fig 4 23 Coefficient Quantitative 0 000 Abs WL 500 0nm 0 000 mg ml Please Input WL 500 0 nm WL 4 Cali
21. s att futile gt Hoc Copies HEI loz to confirm The system will go into the following interface Fig 4 11 500 0nm 0 000Abs 500 0nm 0 000Abs Input Stds No No of Copies No __ Fig 4 11 A Use the numeric keypad or Arrow Key to input the test times of each ENTER standard sample then press to confirm Then the system will go into the following interface Fig 4 12 500 0nm 0 000Abs 0 000Abs Put 1 Standard No of copies Input 1 Conc C TT Fig 4 12 Te 3 Calibrate 100 T 0Abs CO nd Hom EE Test St Put the Reference Solution in the light path then press calibrate 100 I OAbs ilim Tillis ArtiSandad 4 Input Standard Samples Concentration x Pull the 1 Standard Sample in the light path and input the value of Putt Sanda htt Tesi its concentration by the numeric keypad then press to confirm The system will go into the next interface Fig 4 13 1 500 0nm 0 100Abs 500 0nm 0 100Abs Put 1 Standard Input 1 Conc Test Std 1 1 C 0 020 Fig 4 13 1 Press to measure 1 Standard Sample for the first time then the system will ask you to test Sample 1 again Fig 4 13 2 500 0nm 0 100Abs 500 0nm 0 100Abs Test Std 1 1 Test Std 1 2 ENTER Press to measure 1 Standard Sample for the second time Then it goes into the next Interface Fig 4 14 Fig 4 13 2 Note The system will take th
22. sity of the light focused on the A D before it permeates the solution Note When test the solvent is usually taken as the Reference Solution and its Transmittance is considered as 100 T While the Transmittance of the sample to be tested is a relative value which is got comparing to that of the Reference 3 Structure The spectrophotometer consists of five parts 1 Halogen or deuterium lamps to supply the light 2 A Monochromator to isolate the wavelength of interest and eliminate the unwanted second order radiation 3 A sample compartment to accommodate the sample solution 4 Detector to receive the transmitted light and convert it to an electrical signal and 5 A digital display to indicate absorbance or transmittance The block diagram Fig 2 4 below illustrates the relationship between these parts e s _Jofeo s Light sample Process and Monochromator Detector a Pa Loa j H Source Compartment Display Fig 2 4 Block diagram for the Spectrophotometer In your spectrophotometer light from the lamp is focused on the entrance slit of the monochromator where the collimating mirror directs the beam onto the grating The grating disperses the light beam to produce the spectrum a portion of which is focused on the exit slit of the monochromator by a collimating mirror From here the beam is passed to a sample compartment through one of the filters which helps to eliminate unwanted second order radiation from the diffraction
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