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Operating Instructions G 41 - 127 / i-e - Science

1. images Objective Magnification Aperture Working distance mm Cat No Planachromat 2 5 0 08 8 9 46 01 10 9906 F Achromat _ 2 5 0 08 8 9 46 01 05 Achromat 3 2 0 07 23 2 46 01 00 9903 Planachromat 6 3 0 16 4 9 46 03 10 Achromat 10 0 22 5 0 46 04 00 9903 Achromat 10 0 22 Ph 1 5 0 46 04 01 9904 F Achromat 10 0 25 6 8 46 04 05 F Achromat 10 0 25 Ph 1 6 8 46 04 06 Planachromat 10 0 22 4 8 46 04 10 Neofluar 10 0 30 4 8 46 04 20 Planachromat 16 0 35 2 8 46 05 10 Planachromat 16 0 35 Ph 2 2 8 46 05 11 LD EPIPLAN 16 0 30 Pol 4 1 46 21 23 F Achromat LD 20 0 25 2 91 46 06 05 F Achromat LD 20 0 25 Ph 1 2 01 46 06 06 Planachromat 25 0 45 1 4 46 06 10 F Achromat LD 32 0 40 1 02 46 07 02 F Achromat LD 32 0 40 Ph 1 02 46 07 03 LD Achroplan 32 0 40 6 8 44 08 50 LD Achroplan 32 0 40 Ph 2 6 8 4408 51 1 For coverglass thicknesses of 1 2 mm 4 For coverglass thicknesses of 1 2 1 8mm With coverglass thickness of 2 mm the working distance is 2mm With coverglass thickness of 1 5 mm the working distance is 6 8 mm 2 For coverglass thicknesses of 0 5 1 5 mm The following coverglass caps are required to compensate different With coverglass thickness of 1 5 mm the working distance coverglass thicknesses is 1 0 mm Coverglass cap D Q 0 6 44 49 30 for coverglass thicknesses of 0 0 6 mm 3 Screw objective in ID 03 nosepiece via adapter Coverglass cap D 0 6 1 2 mm 44 49 31 for coverglass f 160 mm 44 49
2. where necessary 2 center halogen lamp after loosening screws 6 2 set opimum illumination After setting phase rings remove the centering telescope from the tube and insert the eyepiece If necessary insert interference wide band filter green 46 78 03 in filter opening with holding ring for light filters 46 72 52 3 5 Supporting frames for mechanical stage MT The following supporting frames can be inserted into the mechanical stage MT 3 10 for 96 position microtiter plates 471746 for microtest plates 60 72 120 positions 47 17 47 e g for for HLA tests for multidishes e g 24 position Costar plate 47 17 44 for 76 x 26 mm specimen slide 471719 for Petri dishes dia 36 mm 47 17 42 for Petri dishes dia 54 mm 4717 43 for Petri dishes dia 65 mm 47 17 58 for Petri dishes dia 88 mm 47 17 59 Objectives 6 The following objectives are available in addition to Achromat The parfocal length of the objectives on the nosepiece is 1 3 mm objectives above the stage plane in air i e this plane will always be in focus z F Achromat flatfield objectives when objectives are changed a F Achromat LD flatfield objectives with particularly long working distance Planachromat perfect flatfield objectives also for phase contrast a Achroplan LD perfect flatfield objectives with particularly long working distance Neofluar top of the line fluorite objectives for high contrast
3. 00 thicknesses of 0 6 1 2 mm Eyepieces CPL compensating flatfield eyepieces highly corrected for sophisticated work Kp compensating flatfield eyepieces optimally corrected for most exacting demands Magnification Angular field Cat No Field of view number CPL 10x 18 Br 42 46 40 22 CPL 10x 18Br foc 42 46 40 23 Kpl W 10x 18 Br 41 46 40 42 Kpl W 10x 18 Br foc 2 41 46 40 43 Kpl 12 5x 18 Br 48 46 41 42 Kpl 12 5x 18 Br foc 2 48 46 41 43 Kpl 16x 16 55 46 42 44 1 High eyepoint eyepiece for eyeglass wearers Users who do not wear eyeglasses use the fold down rubber cups 2 Focusing eyepiece Magnification of the microscope M Microscope M objective M eyepiece e g 200 20x 10 where M objective 7 objective magnification 20 in the example M eyepiece magnification 10 in the example eyepiece Lamp exchange 7 a Disconnect microscope from line supply s Open black lamp cap of illuminator 20 to the left Remove defective lamp Use paper cloth to hold new lamp and plug into socket Breathe on lamp and wipe off any finger prints with a clean cloth a The lamp can be focused with 6 1 The lamp can be centered after loosening 6 2 6 Spare fuses for 220 240 V T0 16A 250V 380127 0120 for 100 127 V T0 4A 250V 380142 0285 Two fuses are inserted beside the power plug in the instrument base To exchange them remove bay
4. 0x 18 Br foc with focusing eyelens 46 40 43 9902 7 Binocular tube permanently fixed in position 8 Quadruple revolving nosepiece with screw in objectives see fig 3 9 Focusing control acting on the nosepiece Vertical adjusting range 10 mm 10 Base with built in power supply for 220 240 V 47 12 02 9903 or for 100 127 V 47 12 03 9903 Frequency 50 60Hz Power consumption max 40 VA The instrument is interference free and complies with the VDE IEC and CSA regulations 11 Power switch with brightness control of 6 V 20 W halogen lamp The output voltage is stabilized and electronically adjustable up to 6 V A SS S Ss The Invertoscope ID 03 MT fig 3 is particularly suitable for accommodation of application oriented supporting frames for different specimen holders like microtiter and microtest plates etc see page 5 The mechanical stage MT 3 10 i e fig 3 item 10 is available for screening It is attached to the fixed stage from the left hand side and fixed from below with three knurled Screws Assembly Take stand out of transport case and put it on worktable Loosen two socket head screws 2 5 mm 2 2 of stand with supplied screwdriver pull out stand column 2 1 until it is about 15 cm above the stage plate and tighten socket head screws again The coarse and fine control 47 12 09 is preferably used for the focusing of objectives with magnifications of greater than 20 In case this contr
5. invertoscope ID 03 and ID 03 MI Operating Instructions G 41 127 i e Contents 2 Page Invertoscopes ID 03 and ID OS MT ssssssssssssesseessnsssennrussnnnnosaennrnnnosnessonnnnnninsersserarena 3 ASSEDI aiins anaa AA e AT aenenenien aan 3 Braigntield adjustment scmavinanunraenn a e 4 Phase contrast adjustment ssssessssssssssirssnsssnnnsnenunnatnosseennennrensstosanonarennenaniesnrasnee 5 Supporting frames for mechanical Stage MT ss seeessssssessensssessresseroseessrisnrarerernrrenns 5 ODISCIIVGS seataceresavassancevteisascesvessanectere tatiana aca aarrinuaamm aut 6 VCC COS nain E OT 7 Magnification of microscope n E EER PRE ae aed maser A ER 7 EAMIDOXCNANGE ia E A dante 7 Notes The 6 or 10 digit numbers e g 47 17 45 are ordering numbers for instruments or components Changes or repairs to these instruments may only be performed by the manufacturer or by a person expressly authorized by him to do so Specifications subject to change Invertoscopes ID 03 and ID 03 MT 3 Fig 1 invertoscope ID 03 1 Lamp housing 6V 20W with LD condenser 0 3 Br Ph 1 Ph 2 and lamp carrier connected to in base power supply 2 Clamping screw to secure the lamp carrier 3 Fixture with clamping screw and pin to align the lamp carrier The illumination system can be rotated about the stand column 4 Fixed specimen stage 211x270 mm 5 Kpl wide angle eyepiece 10x 18 Br 46 40 42 9903 6 Kpl wide angle eyepiece 1
6. ol is packed separately from the microscope it can be mounted by the user himself put the coarse and fine control to the prepared drive axle so that its pin engages into the groove of the drive axle Tighten clamping screw with supplied screwdriver width of opening 2 5 For subsequent installation please notify our nearest agency Brightfield adjustment Connect microscope to line supply knob 1 11 must be switched off Then switch on halogen lamp with this knob Swing an objective into light path Place specimen on the specimen stage When using an Invertoscope ID 03 with mechanical stage MT 3 10 slide supporting frame 3 13 e g for microtiter or micro test plates beneath spring clips 8 11 from the front until it snaps into position Unless not yet done stick scales to the recesses of the mechanical stage i e the scale with numbers 3 9 in the direction left right and the scale with letters 3 12 in the direction to fro Focus on the specimen Adjust tubes 3 16 until you see a circular sharply defined field of view with both eyes Look through eyepiece 3 17 and focus on the specimen with contro 3 14 Then focus for the other eye by turning the eyelens 3 18 of the eyepiece Illuminate the specimen Adjust height of illuminator 20 3 4 with lamp carrier 3 1 and holding ring 3 2 until the distance between specimen and underside of illuminator is approx 56 mm Insert diaphragm slide 3 3 Remove
7. onet mount and replace defective fuses
8. safety screw 3 8 from slide Insert slide into opening of the illuminator from the right until stop in the middle position Fasten safety screw Illuminate objective pupil uniformly and brightly Remove eyepiece from tube Observe objective pupil either with the naked eye or with a centering telescope adjust the eyelens until the objective pupil is in focus Swivel illuminator 20 slightly around column and clamp it when illumination is at its brightest and uniform Fix safety ring 3 2 on lamp carrier The condenser front lens 47 12 08 3 7 that can be attached to the illuminator increases the aperture of the LD condenser of the illuminator to 0 6 The distance to the specimen is then approx 15 mm Adjust brightness with control 1 11 Set optimum image contrast with lever 3 6 Where necessary insert filter dia 32 mm into filter opening of illuminator 20 with holding ring 3 5 Phase conitrast adjustment Screw Ph objectives into nosepiece Slide phase stop Ph 1 4 1 into light path for objectives Ph 1 and phase stop Ph 2 4 2 for objectives Ph 2 Adjust specimen as described for brightfield Insert centering telescope in binocular tube instead of an eyepiece and adjust its eyelens until the dark ring is in focus Use centering screws 4 4 to cover black Ph ring with bright Ph ring fig 5 If the bright Ph ring is not uniformly illuminated then 1 check exact center position of illuminator 20 and readjust

Operating Instructions G 41 - 127 / i-e - Science

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