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EASY-nLC Series Troubleshooting and

1. Solvent A check valve Solvent B assembly check valve assembly 7 AN Autosampler needle al Four 3 position Valye S Valve W Va 6 port valves 6 6 Pressure sensor S h Ls O O l Mixing Tee Pressure sensors A and B Three syringe pumps Lo e P Flow sensors A and B Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 7 1 Introduction Hardware Components Syringe Pumps The EASY nLC instrument contains three syringe pumps The binary liquid chromatography system consists of two high pressure pumps pumps A and B The automated sampling system uses syringe pump S to draw sample from a sample vial or well and load it into the sample loop attached to valve S Figure 8 shows the EASY nLC syringe pump components The PLF model is installed in the EASY nLC II instrument The PLU model is installed in the EASY nLC 1000 instrument and is supplied as spare part for both instruments Each syringe pump consists of these primary components a stepper motor a pump body a pump head a piston a piston seal and a backup ring The PLU model also has an LED panel and a pump printed circuit board PCB cover The piston seal is the only user replaceable component The pump head holds up to 140 pL of fluid The backward movement of the pis
2. No Issue code name Description Cause Solution 41 ERROR_SENSIRION_ Cannot store flows larger Abnormal value trying Cycle EASY nLC power CALIBRATION than 9999 nL min to be written to the Check flow sensor flow sensor functionality 42 ERROR _ RS232_INIT Initialization of interface Access to interface Cycle EASY nLC power PORT_NAME failed failed 54 ERROR RS485_ COMPONENT_ID Error occurred when Troubleshoot P BUS COMMAND_VERIFY COMMAND failed sending COMMAND communication hardware ERROR_MESSAGE 55 ERROR_ Unknown rack shelf Unknown rack shelf Verify that the sample MANIPULATOR_ RACK_SHELF_VALUE position supplied to position is correct UNKNOWN_ RACK _ driver SHELF 56 ERROR_ Unknown rack position Could not move to bad Verify that the sample MANIPULATOR_ id rack position position is correct UNKNOWN_RACK_ RACK_POSITION_ID_ POS_ID VALUE 57 ERROR Grid configuration Error uploading Calibrated position not MANIPULATOR_SET___ change failed autosampler plate recognized by software See GRID_CONFIG L AUTOSAMPLER_ calibration Chapter 7 Calibrating the RETURN_CODE 0 asrOK Autosampler s XYZ Robot 1 asrBusy 2 asrBadLocation 3 asrBadCoordinate 4 asrEjected 5 asrAxisError 6 asrChecksumError 58 ERROR_ Wash configuration Error while saving Calibrated position not MANIPULATOR_SET___ change failed autosampler wash recognized by software See WASH_CONFIG AUTOSAMPLER_ calibration see code 57 Chapter
3. 2013 Thermo Fisher Scientific Inc All rights reserved EASY nLC AFC IFC nanoViper and PepMap are trademarks and Acclaim Dionex LCQ Fleet LXQ LTQ and Xcalibur are registered trademarks of Thermo Fisher Scientific Inc PEEK is a trademark of Victrex PLC PEEKsil is a trademark of SGE International Pty Ltd Corp This product uses FreeRTOS from www freertos org available upon request FreeRTOS is a registered trademark of Real Time Engineers Ltd The following are registered trademarks in the United States Advion and RePlay are registered trademarks of Advion BioSystems Inc CMOSens is a registered trademark of Sensirion AG Corp Duran is a registered trademark of Schott AG PhotoMOS is a registered trademark of Panasonic Electric Works Co Ltd Teflon is a registered trademark of E I du Pont de Nemours and Company VICI and Valco are registered trademarks of Valco Instruments Co Inc The following are registered trademarks in the United States and other countries Microsoft and Windows are registered trademarks of the Microsoft Corporation SSH is a registered trademark of Tectia Corporation Linux is a registered trademark of Linus Torvalds individual All other trademarks are the property of Thermo Fisher Scientific and its subsidiaries Thermo Fisher Scientific Inc provides this document to its customers with a product purchase to use in the product operation This document is copyright protected and any reproductio
4. EZL pg 6 After the EASY nLC application finishes the script do the following a Eject the tray b Check that 2 pL is left in the well or vial by reweighing the microplate or vial or by checking the volume with a pipette c If more than 2 pL remains recalibrate the autosampler run the Flush Air script and then rerun the sample pickup check For information about calibrating the autosampler see Chapter 7 For information about the Flush Air script see Prepare Flush Air on page 33 Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 263 6 Troubleshooting Troubleshooting Communication Problems Troubleshooting Communication Problems To troubleshoot communication problems see these topics e Network Access on page 264 e Network Connection Failures on page 265 e Testing the Network Connection to the EASY nLC Computer on page 266 Network Access Table 25 contains troubleshooting tips for network access problems Table 25 Network access problems Symptom System is not responding after pressing Save Configuration on the Network page under Configuration Possible causes Length of completion time to update the network settings Action As indicated wait while the system changes the network settings Cannot access the EASY nLC system through the network 264 Changed network address Check the network address of the EASY
5. Figure 47 Purge solvent script with 0 iterations Thermo Home Batch Setup Method Editor SCIENTIFIC Scripts Category Name f Prepare hg f Purge solvent X Log Book Support Description Output Devices Job 00 00 00 2 May 2011 18 26 admin Schedule Exit 6 Select the check box for the appropriate pump 7 Press Start Configuration Fio graph Fio graph The piston moves backward until it reaches the 140 pL position 8 To replace the piston seal go to the piston seal replacement procedure for your pump e Replacing the Piston Seal and Cleaning the Piston in a PLF Pump on page 62 or e Replacing the Piston Seal in a PLU Pump on page 67 Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 61 4 Routine Maintenance Maintaining the Syringe Pumps Replacing the Piston Seal and Cleaning the Piston in a PLF Pump Most EASY nLC II instruments have PLF model pumps see Figure 8 on page 9 Note To replace the piston seal in a PLU pump follow the instructions in Replacing the Piston Seal in a PLU Pump on page 67 Replacing a piston seal in the PLF pump requires these tools and materials Tools Parts and materials e 2 Phillips head screwdriver e Powder free safety gloves e 13 mm open ended wrench e LC MS grade methanol e 1 4 in open ended wrench e LC MS grade acetonitrile e Pipette e Piston seal and piston
6. _ lt _ left 5 Using the step buttons in the Needle Up Down area 5 adjust the needle height as follows a Using the big step button WV lower the needle until it reaches a level of 2 3 mm 0 08 0 12 in above the bottle b Using the small step button J slowly let the needle enter the hole and lower the needle until it reaches the bottom of the wash insert in the bottle Note The O ring at the top of the needle holder rises a little when the needle reaches the bottom of the well c Press the large step button T7 twice to raise the needle off the bottom of the wash bottle insert Two large steps higher than the bottom of the wash bottle insert is the appropriate needle height Note The ASA autosampler has a limit of 850 steps on the z axis vertical This limit is set to avoid bent needles You might be able to go for more than 850 steps during calibration but the needle does not go more than 850 steps during normal work 6 Press Capture when you are satisfied with the current xyz coordinate for position W4 The color of the bottle container number changes from green to red 7 Optional To calibrate the extra vial positions for the 6 x 8 plate format do the following a Press V1 vial 5 to select it b Repeat step 4 through step 6 for vial V1 8 Press Save and then press Close to close the calibration dialog box 286 EASY nLC Series Troubleshooting and Maintenance Guide
7. This script is only valid for EASY nLC II instrument valves with a serial number less than V 009999 Contact your local Thermo Fisher Scientific field service engineer if the reported overshoot is greater than 6 Unhandled error or SVG Program error in the software scalable vector graphics error release Thermo Scientific In most situations you can continue your work by pressing Ignore or OK More serious error situations might require restarting the HPLC To improve on the software quality e mail details to us customer support analyze thermofisher com eu techsupport cms thermofisher com Or connect to the remote support server See Connecting the EASY nLC Instrument to the Support Server on page 288 EASY nLC Series Troubleshooting and Maintenance Guide 203 6 Troubleshooting Using the Direct Controls for Troubleshooting and Maintenance Using the Direct Controls for Troubleshooting and Maintenance You can access the direct controls for the instruments hardware components by pressing the component icons on the Home gt Overview page These topics describe how to use the direct controls e Using the Valve Controls e Using the Pump Controls e Using the Autosampler Controls Using the Valve Controls You can change the positions of the instrument s three position valves from the Home gt Overview page For information about the valve positions see Six Port Rotary Valves on page 12
8. Using a 9 64 in L hex wrench tighten the two hex screws a little at a time by shifting from one screw to the other and back again until the screws are evenly torqued Mount the right side panel to the instrument housing Then with a 2 Phillips head screwdriver secure the panel by rotating the three captive screws a quarter turn clockwise Turn on the EASY nLC instrument and log in as an administrator or a super user Go to the next procedure To remove air from the system and check for leaks after disassembling a valve To remove air from the system and check for leaks after disassembling a valve If you removed the stator from valves A or B flush air from the system as follows a Set up the Flush Air script as described in Prepare Flush Air on page 33 b Select the pump that is connected to the affected valve c Set the flush volume threshold to 10 pL for the EASY nLC II or 12 pL for the EASY nLC 1000 d Press Start e Wait for the script to finish Check the system or subsystem for leaks as follows e Ifyou removed the stator from in valve A or B run the Leaks script for the affected valve see Test Leaks on page 44 e Ifyou replaced the rotor seal in valve S or W run the Leaks script for the system When the system is leak tight the instrument is ready for use EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 4 Routine Maintenance Maintain
9. Valve check X Support Devices fre graph Job 00 15 48 11 May 2011 12 47 admin To run the Valve Check script 1 Select the Valve Check script as follows a Press Maintenance gt Scripts b In the Category list select Test c Inthe Name list select Valve Check 2 Set up the parameters for this script as follows a Press the Parameters tab b Select the check boxes for the valves that you want to test 3 Press Start Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 47 3 Maintenance Scripts and Service Records Maintenance Scripts Test Back Pressure 48 EASY nLC Series Troubleshooting and Maintenance Guide The Back Pressure script determines instrument back pressure for solvent A and B The script runs at a preset flow and measures the back pressure on the system IMPORTANT Before you perform the Back Pressure script ensure that the solvent A bottle contains water and the solvent B bottle contains acetonitrile This test is not valid for other solvents Use this script to test for a blockage in the solvent system Before running the script connect the Column Out line to the Waste In line as shown in Figure 35 on page 45 Figure 37 shows the parameters for the Back Pressure script Figure 37 Back Pressure script parameters Thermo Home Batch Setup Method Editor SCIENTIFIC Log Book Ge tack pressure Support Configuration
10. e 110 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific Thermo Scientific 5 Field Service Maintenance Replacing the Built In Computer 6 Using a Torx T 10 wrench remove the four screws that secure the computer box to the instrument see Figure 101 Figure 101 Screws to remove from the computer box 7 Pull the computer box out of the EASY nLC housing as shown in Figure 102 Figure 102 Pulling out the computer box nr EASY nLC Series Troubleshooting and Maintenance Guide 111 5 Field Service Maintenance Replacing the Built In Computer R kod I 2 To install the new computer box Insert the new computer box into the EASY nLC housing Reconnect all of the internal cables to the computer box e Figure 97 on page 109 shows the connections to the computer box inside the EASY nLC 1000 instrument e Figure 98 on page 109 shows the connections to the computer box inside the EASY nLC II instrument Using a Torx T 10 wrench and the four screws that you removed in step 6 on page 111 secure the computer box to the instrument see Figure 101 on page 111 Using a Torx T 10 wrench and the screws that you removed in step 5 on page 110 secure the back panel to the instrument Tip Do not reconnect the left side panel to the instrument housing until after you recalibrate the autosampler Reconnect the cables to the back panel as follows e Reconnect the pow
11. 7 Calibrating the Autosampler s XYZ Robot Calibrating Plates Figure 248 Plate calibration captured for the A1 position f Autosampler Plate Calibration Calibrate Plate HT MTP Needle In Out gt Needle Up Down Needle LeftRight 7 Press the green vial or well in the lower right corner of the plate view The needle moves to the current saved coordinate The saved coordinates for the x and y axis appear on the left side of the slash the needle does not descend into the vial or well and the z axis value on the left side of the slash remains at 0 8 Repeat step 4 through step 6 on that vial or well 9 Press Save and then press Close to close the calibration dialog box 10 Replace vial caps and plate covers as necessary 11 Go to the next procedure to calibrate the W4 bottle position or reinstall the EASY nLC instrument s left side panel IMPORTANT To maintain a stable temperature inside the tray compartment the left side panel must be installed 284 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 7 Calibrating the Autosampler s XYZ Robot Calibrating the Wash Bottle and Extra Vial Positions Calibrating the Wash Bottle and Extra Vial Positions In addition to holding the sample trays the EASY nLC tray compartment holds four wash bottles W1 W4 and six extra vials V1 V6 To calibrate the W4 wash bottle position the extra vial positions or both 1 If
12. Bus cable disconnected from valve B Horizontal cable bar Back side of the Right side of the autosampler autosampler 9 Using a 3 mm hex wrench remove the three screws that secure the autosampler to the bottom plate of the housing see Figure 119 Figure 120 and Figure 121 e Remove the screw from the right side of the autosampler see Figure 119 Figure 119 Removing the screw from the right side of the autosampler Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 125 5 Field Service Maintenance Replacing the ASC Autosampler e Remove the screw from the back left side of the autosampler see Figure 120 Figure 120 Removing the screw from the back left of the autosampler e Remove the screw the screw on the front of the autosampler see Figure 121 Figure 121 Removing the front screw from the autosampler 10 Remove the autosampler from the housing through the back of the instrument Figure 122 shows the housing with the autosampler partially removed Figure 122 Housing with autosampler partially removed 126 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific Thermo Scientific 10 11 12 5 Field Service Maintenance Replacing the ASC Autosampler To install the ACS autosampler into an instrument that previously included this model Insert the new autosampler into the housing Using a 3 mm L hex wrench and if necessary a needle nosed
13. Daily Maintenance Check the solvent levels draw fresh solvent through the solvent system and flush air out of the solvent system on a daily basis To check the solvent levels 1 Visually inspect solvent bottle A solvent bottle B and the autosampler bottle in position W3 and the bottles in position W1 and W2 if used Refill if necessary Tip You can also inspect the purity of the solvent to ensure no visible precipitates have formed that might lead to blockages in the pump line 2 Visually inspect the autosampler wash bottle in position W4 and the plastic waste beaker in front of the solvent system compartment and empty if necessary To draw fresh solvent into the system and to flush air out of the system Run the Purge Solvent scripts with two purge iterations and run the Flush Air script until flush volumes fall below 10 pL for the EASY nLC II instrument or 12 pL for the EASY nLC 1000 instrument Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 27 2 Maintenance Schedule Weekly Maintenance Weekly Maintenance Because the vapor pressures of formic acid water and acetonitrile differ the solvent composition changes over time Refill the solvent bottles to maintain a consistent solvent composition Quarterly Maintenance Perform the following procedures quarterly every 3 months To check the back pressure To test for system blockages run the Back Pressure script for both solv
14. EASY nLC Series Troubleshooting and Maintenance Guide 139 5 Field Service Maintenance Replacing the Autosampler Cooler Replacing the ASA Cooler Replacing the ASA cooler in an EASY nLC II instrument requires these items Tools Parts 2 mm L hex wrench or ball driver ASA autosampler cooling module e 2 5 mm L hex wrench or ball driver pi Eee 3 mm L hex wrench e 5 5 mm open ended wrench e 2 Phillips screwdriver To replace the ASA cooler follow these steps 1 To remove the ASA cooler on this page 2 To install the ASA cooler on page 145 lt To remove the ASA cooler 1 Close down and turn off the EASY nLC instrument see Closing Down the EASY nLC Instrument on page 21 Then disconnect the power cable 2 Remove the autosampler door shown in Figure 144 to avoid damaging it when you remove the hex screws underneath the adapter plate holder Figure 144 Autosampler door 140 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 5 Field Service Maintenance Replacing the Autosampler Cooler 3 Depending on the instrument model do one of the following e On the EASY nLC II model LC100 use a 5 5 mm open ended wrench to loosen the nuts underneath the door see Figure 145 CAUTION Be careful removing the lid because the spring on the door can snap back Figure 145 Nuts beneath the lid of the EASY nLC I instrument e On the EASY nLC II model LC100 2 use a 2 mm L
15. Figure 1 EASY nLC II instrument on the left and EASY nLC 1000 instrument on the right To familiarize yourself with the EASY nLC instrument and its instrument control software review these topics Contents e Hardware Components e Touch Screen Application e Specifications Note For information about setting up the EASY nLC instrument installing the columns creating methods and running batches refer to the EASY nLC Series Getting Started Guide Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 1 1 Introduction Hardware Components Hardware Components These topics describe the hardware components of the EASY nLC instrument e Autosampler e Solvent System Components Behind the Right Side Panel e Computer and Autosampler Behind the Left Side Panel e Back Panel Autosampler The autosampler module of the EASY nLC instrument is located behind the tray compartment door You can open the tray compartment door manually or by using the touch screen controls With the use of a vial adapter plate or a microwell adapter plate the tray compartment can hold sample vials a 96 well microplate or a 384 well microplate The robotic unit moves the z axis needle holder along the x and y axes to the user specified sample location during a run When the autosampler needle reaches the sample location it descends into the sample vial or microplate well IMPORTANT Because they can block the movement of th
16. Figure 204 shows the pink pump icons on the Home gt Overview page Figure 204 Pink pump icons on the Home gt Overview page Thermo seleuTirie ASIEN Batch Setup Method Editor Maintenance Configuration Graphs LSP disabled Idle flow disabled A nl min A Fi oo oo oo oo oo oo Queue Initialize system Equilibrate precolum n Prealign A B Equilibrate analytical column Gradient Autosampler wash refill Job 00 00 00 Insert plate Ea Sample Job Current 31 Oct 2011 08 45 admin EASY nLC Series Troubleshooting and Maintenance Guide 199 6 Troubleshooting Troubleshooting Tips A pink valve icon means that the communication to the unit has been lost Be aware that the valves are serially connected so a failing valve might cause other valve icons to turn pink as well Communication between the valves and the embedded computer can be lost when the cables are loose when the embedded computer fails or when the valve PCB fails Figure 205 shows the pink valve icons on the Home gt Overview page Figure 205 Pink valve icons on the Home gt Overview page Thermo SCIENTIFIC A s Configuration 0 0 ni min Idle flow disabled 140 0 140 0 yl bats i Graphs Queue 0 7 bar 0 0 ni min 140 0 140 0 pl Solvent A 0 6 bar 0 0 ni min 140 0 140 0 pl Pickup sam pl Load sample u tum in Prealign A B equilibrate analytical column olvent B Current Gradient Autosampler wash refill 31 O
17. system plumbing is set up as shown in Figure 223 on page 239 with the solvent channel B disconnected from the mixing Tee Follow the workflow in Figure 224 or the procedure on pages 241 242 to locate and remedy the leak Figure 224 EASY nLC 1000 workflow for locating a leak between flow sensor A and the mixing Tee Part 1 You determined that the leak is in the solvent path between the outlet of flow sensor A and the mixing Tee inlet Disconnect the solvent line from the outlet of flow sensor A Then block the outlet with a blind fitting step 1b on page 241 Install a new solvent line between the flow sensor A outlet and the mixing Tee Readback inlet step 1d on page 242 for flow sensor A Then reconnect all of the solvent lines and gt 100 nL min rerun the system leak test page 218 Yes Using a 5 16 in open ended wrench tighten the inline filter to the outlet of flow sensor A step 2a on page 242 Readback for flow sensor A gt 100 nL min Reconnect all of the solvent lines and then rerun the system leak test page 218 Replace the inline filter page 81 Then reconnect all of the solvent lines and rerun the system leak test page 218 240 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 6 Troubleshooting Troubleshooting the Results of the System Leak Test To locate and remedy a leak between flow sensor A and the mixing Tee in the EASY nLC 1000 1
18. 250 0 250 0 250 0 250 0 gt gt 200 0 200 0 2 amp 200 0 200 0 2 E 150 0 150 0 5 150 0 150 0 5 100 0 100 0 G amp 100 0 H 100 0 50 0 0 0 50 0 50 0 0 0 0 0 6 0 M 0 5 10 15 20 0 5 10 15 20 Time min Time min 300 0 cafe Se Hs Desired flow Pressure 90 0 300 0 Job 00 43 13 250 0 80 0 Sample 00 21 17 I 700 amp post Tea Gradient 11 06 E 2000 soo 3 200 0 E 1500 500 g gt z z ae 00 150 0 5 19 ut 2011 2 1008 300 B z 100 0 F 20 0 S 50 0 50 0 admin 10 0 0 0 6 0 0 0 0 5 10 15 20 0 5 10 15 20 Time min Time min Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 181 6 Troubleshooting Troubleshooting Tips Figure 189 shows the flow path between pump B and the mixing Tee Figure 189 Solvent lines between pump B and the mixing Tee Valve B Flow sensor B Connected to check valve assembly B No EASY nLC Il instrument EASY nLC 1000 instrument 1 Stainless steel tubing and fittings Stainless steel tubing and fittings 2 Stainless steel tubing and fittings Stainless steel tubing and fittings 3 PEEKsil tubing nanoViper e Stainless steel fitting connected to port 6 of valve B e PEEK fitting connected to the flow sensor inlet 4 Fused silica solvent line nanoViper e PEEK fitting connected to the flow sensor outlet PEEK fitting connected to the mixing Tee 182 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific
19. 3 Maintenance Scripts and Service Records Maintenance Scripts Prepare lsocratic Flow The Isocratic Flow script runs solvents A and B at a fixed mixture ratio The script uses the AFC system to accurately control the flow Use this script to tune the mass spectrometer at a given B percentage or when cleaning the instrument the columns or both Figure 28 shows the parameters for the Isocratic Flow script Figure 28 lsocratic Flow script parameters Thermo Home Batch Setup icidgeemacineem Maintenance Bi teladtelie imieia SCIENTIFIC p fi eH No graph X Log Book Prepare isocratic flow X Support Output Devices Parameter v Value f Job 00 15 48 11 May 2011 12 41 a admin Schedule Exit Torun the Isocratic Flow script 1 Select the Isocratic Flow script as follows a Press Maintenance gt Scripts b In the Category list select Prepare c Inthe Name list select Isocratic Flow 2 Set up the parameters see Figure 28 for this script as follows a Press the Parameters tab b Enter the appropriate values In the Volume box pL enter the total volume to be delivered by the pumps Note If you select the Run Indefinitely check box the software ignores this volume setting e In the Flow box pL min enter the flow rate to be used e In the AB Mix box B enter the solvent composition as a percentage of solvent B e Ifyou want the solvent flow t
20. Sample Signal Weak or Absent 6 Troubleshooting Troubleshooting Tips If the sample signal is missing or weak review the troubleshooting tips in Table 15 Table 15 Possible causes of a weak or absent signal Symptom Possible cause The sample signal is missing or Sample not aspirated weak See Figure 190 on page 184 Action Run the Sample Pickup script If the Sample Pickup script fails see Troubleshooting the Autosampler Aspiration and Calibration on page 261 Damaged or incorrect column See Figure 191 on page 185 Leak in the system See Figure 192 on page 186 Make sure that you have installed the correct column or columns Check the back pressure on the columns by running the column equilibration scripts Run the Leaks test on the system If the test fails see Troubleshooting the Results of the System Leak Test on page 221 Incorrect column configuration Check the column configuration 1 Press Maintenance gt Devices 2 In the Devices list select EASY nLC HPLC 3 Press the Properties tab 4 Clear the One Column Setup check box for a two column setup or select this check box for a one column setup For more information refer to the EASY nLC Series Getting Started Guide Incorrect solvent in W3 bottle Make sure that the W3 bottle contains solvent A Incorrect sample loop configuration Thermo Scientific Check the loop configuration on the Propertie
21. TEAR HE N RAY FE ARRAY LIK a a E Contents it ee ee ee ee er eer ere re Ter xix Related Documentation ssn unus s sunun n he ha EO ee ROHS EES xX Downloading Manuals from the Customer Manuals Web Site XX Safety and Special Notices i 5a ccad pae owe dbdasea dala chseake teemane xxi Contacting Us ny cica cites phe ids Cha ea bin Mirae Mand awe xxiii Chapter Introduction pccccaneweaccrriceebarestverevedenseataeratenteasestaneders 1 Plaka wate Comp nentss esasen rctu raion eR iia rdenseuraeecheraien 2 A tosample se tia Macias tie theta leekiie be eed eases 2 Solvent System Components Behind the Right Side Panel 5 Computer and Autosampler Behind the Left Side Panel 15 Back Panels norice tao r tieid eee e oe ete che hee as Kees 16 Touch Screen APONCANOM use hind peeks Rake Soe hd ee ERE hee 18 Description of the Touch Screen Monitor 000000 e eee eee 18 Logging In to the EASY nLC Instrument for Maintenance Tasks 20 Closing Down the EASY nLC Instrument oxen cede cae ene cede 21 Specifications i crecer eripere EEE ETE E E E E enna 23 Performance Speciicanons yeckveren acters Kies cannes deemed 23 Technical Specifications civ cis cdo vowel air elie tees ehe eu ees 24 Chapter2 Maintenance Schedule 0 c ee eee eee eee erent e eens 27 Daily Maintenance 0 eee cence eee e eens 27 Weekly Maintenance 1 6 eee ene E e ees 28 Quarterly Mainten
22. When you use the EASY nLC instrument as an inlet to an MS detector the LC MS system might require two or more fourplex outlets The interconnected electrical outlets must have a common point to one ground connector Connecting the hardware to external grounds at different potentials can create a ground loop that causes noise and interference and can damage the contact closure relays on the instrument s back panel IMPORTANT The EASY nLC instrument the MS or LC detector and the optional data system hardware must have a common ground for several reasons e Improper grounding or no grounding can result in shock and fire hazards in case of instrument malfunction e Connecting the hardware to external grounds at different potentials can create a ground loop that causes noise and interference e Improper grounding or no grounding can damage the contact closure relays on the instrument s back panel Fixing damaged relays requires the replacement of the instrument s built in computer CAUTION Electrical safety regulations require that the EASY nLC instrument be grounded to prevent electrical shock and a fire hazard in case of malfunction To verify a common ground between the EASY nLC instrument and external hardware 1 Connect the EASY nLC instrument to line power but do not turn on the instrument 2 Using a multimeter measure the resistance between the EASY nLC chassis and the external hardware chassis for example
23. frs graph braid Parameters Bomi Devices Parameter v Value est solvent A est solvent B fro graph Job 00 15 48 11 May 2011 12 50 Exit To run the Back Pressure script 1 Open the Back Pressure script as follows a Press Maintenance gt Scripts b In the Category list select Test c Inthe Name list select Back Pressure 2 Set up the parameters for this script as follows a Press the Parameters tab b Test solvent A solvent B or both A and B by selecting the corresponding check box or check boxes 3 Press Start Thermo Scientific 3 Maintenance Scripts and Service Records Maintenance Scripts Test Autosampler Torque The Autosampler Torque script measures the torque required to move the XYZ robot on each of its axes for the ASC autosampler IMPORTANT Do not run this script for the ASA autosampler Contact your local Thermo Fisher Scientific representative before running this script Figure 38 shows the Description page of the Autosampler Torque script Figure 38 Autosampler Torque script description Thermo Emm SCIENTIFIC scripts il poe pe f Cc f No graph X Log Book Test Autosampler torque Support Batch Setup Method Editor tenance Configuration Parameters Output Devices Tests the individual torque required for the autosampler manipulator axes No graph Job 00 01 02 24 aug 2010 17 50 admin Ex
24. gh_110414132511 Open zwei Deall ael maS Dell lm ample Typ File Name Sample ID Path Inst Meth Proc Meth Posi Status Acquisition Queue Unknown Bl nkUDGI A2 C Xcalibur D C Xcalibur m Al Run Manager Unknown BlankUDG2_ A3 C Xcalibur D C Xcalibur m A2 Control Device Started Unknown BlankUDG3 A4 C Xcalibur D C Xcalibur m A2 Sequence C Xcalibur data PN 2011 4pr Unknown A5 C Xcalibur D C Xcalibur m A2 Sample Name Working On Seq Row 1 Position amp 1 Raw File C Xcalibur D ata PN 2011 April Inst Method C Xcalibur methods PN malta ALN Thermo EASY nLC LTQ Velos MS The device Thermo EASY nLC reported an error while acquiring Waiting for Contact Closure Th acalasition tll Conese The sequence has been paused To resume the list go to the Actions main menu and uncheck the Pause Analysis menu item sy admin 10 32 134 15 LC 000550 190 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 6 Troubleshooting Troubleshooting Tips Figure 198 shows the Acquisition Server message that appears during the Prepare for Run step when the requested injection volume is greater than the configured loop size minus 2 pL Figure 198 Incorrect injection volume A requested injection volume of 20 uL is too large for a 20 uL loop u velos 10 32 134 20 Remote Desktop 4 Thermo Xealibur Sequence Setup gh_110414132511 Open awet Dalla el LLIB Lo Dele m 2 ample Ty
25. 5 Field Service Maintenance Replacing a Pump PCB Replacing a Pump PCB in the PLF Pump Replacing a pump PCB PLF model pump in the EASY nLC II instrument requires these items Tools Parts e 2 Phillips screwdriver Pump PCB P N LC274 e 2 5 mm hex wrench e Small flathead screwdriver To replace the PCB on a PLF pump follow these steps 1 To remove the pump PCB from the PLF model pump EASY nLC II instrument 2 To install the pump PCB on a PLF pump EASY nLC II instrument To remove the pump PCB from the PLF model pump EASY nLC II instrument 1 Close down the EASY nLC II instrument as described in Closing Down the EASY nLC Instrument on page 21 2 Disconnect the power cable from the instrument s back panel 3 Using a 2 Phillips head screwdriver with a quarter turn loosen the three captive screws that secure the right side panel to the instrument housing Then remove the panel 4 Disconnect the P Bus communication cables see Figure 169 on page 158 from the pump actuator Unscrew and remove the four 2 5 mm hex screws that connect the PCB to the pump Disconnect the pressure sensor grounding cable yellow wire N A WwW Gently slide the PCB outward and turn it around to gain access to its back side 8 Disconnect the pressure sensor communication cable Take care to avoid putting any stress on the cables 9 Disconnect the motor cable Again take care to avoid putting any stress on the cable
26. Note The screw in the lower right also connects the pressure sensor grounding cable to the PCB 8 Reconnect the P Bus communication cables to the pump see Figure 108 on page 118 9 Mount the right side panel to the instrument housing Then with a 2 screwdriver secure the panel by rotating the three captive screws a quarter turn clockwise 10 Turn on the EASY nLC II instrument and log in as an administrator see Logging In to the EASY nLC Instrument for Maintenance Tasks on page 20 11 To draw fresh solvent into the pump purge the pump five times by using the Purge Solvent script on the Maintenance gt Scripts page see Prepare Purge Solvent on page 32 12 To remove air from the system run the Flush Air script for the pump associated with the new pressure sensor see Prepare Flush Air on page 33 Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 121 5 Field Service Maintenance Replacing the ASC Autosampler Replacing the ASC Autosampler The procedure for replacing the ASC autosampler is the same for the EASY nLC 1000 instrument and the EASY nLC II instrument However if your EASY nLC instrument has an installed ASA autosampler that is irreversibly damaged you must upgrade to an ASC autosampler because the ASA autosampler is no longer available For instructions on upgrading to an ASC autosampler see Upgrading from an ASA Autosampler to an ASC Autosampler on page
27. TIMEOUT 74 ERROR PARALLEL _ Contact closure port Contact Thermo Fisher PORT_INIT IO error Scientific 75 ERROR PARALLEL _ Contact closure port Contact Thermo Fisher PORT_WRONG_PORT IO error Scientific 77 ERROR_DEVICE_LOW DEVICE Low voltage see issue description Faulty 24V supply _24 VOLT_SUPPLY VALUE V Connect to remote support 78 ERROR_ DEVICE Init failed not see issue description Autosampler hardware MANIPULATOR_INIT ready within 1 minute issue Cycle EASY nLC power If the problem persists check the P bus cables to the autosampler 79 ERROR DEVICE SELF DEVICE Status bit s set Error returned from Connect the system to the _TEST after self test hardware component remote support server see OxBITS_IN_HEX firmware Chapter 8 308 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific Table 30 Error codes Sheet 7 of 8 A Error Codes No Issue code name Description Cause Solution 80 ERROR DEVICE_IN_ DEVICE Device in boot Error returned from Contact Thermo Fisher BOOT_MODE mode after device reset hardware component Scientific firmware because a PCB is damaged 81 ERROR_PELTIER_ DEVICE Spurious Cooler sensor defective Replace the cooler SPURIOUS_ temperature TEMPERATURE TEMPERATURE_IN_ See Replacing the DEGREES_ CELSIUS Autosampler Cooler on page 137 82 ERROR _DEVICE_LOW DEVICE Low current Peltier element Replace the cooler _CURRENT consumption defective
28. The Overview page of the Home menu opens 2 Make sure that the area in front of the tray compartment is clear and then press Eject Plate The tray compartment opens Figure 237 shows the vial adapter plate mounted to the tray holder The four studs on the bottom side of the plate fit snugly into the mounting holes on the tray holder Figure 237 Open tray compartment 3 Remove the current adapter plate by pulling it up and away from the tray holder see Figure 238 Figure 238 Removing the vial adapter plate before installing the microwell plate adapter plate Mounting stud 4 Install the other adapter plate in the autosampler Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 271 7 Calibrating the Autosampler s XYZ Robot Replacing the Autosampler Adapter Plate Replacing the Adapter Plate in the ASA Model Autosampler The ASA model autosampler is the original autosampler installed in the EASY nLC II system To replace the adapter plate in the ASA autosampler 1 Eject the tray as follows a Press Home gt Overview b Press Eject Plate Figure 239 shows an ejected tray Figure 239 Tray compartment of the ASA model autosampler 2 Using the 2 5 mm hex wrench provided with your system remove the screws that secure the plate to the tray The adapter plate is mounted with 4 or 6 hex screws as shown in Figure 240 Figure 240 Screws that secure the adapter plate to the tray 4 hex scr
29. The pump and valve compartment behind the right panel of the EASY nLC instrument contains all the flow lines between the components In addition to pumps and valves the solvent delivery and waste system includes several filters and four check valves The two check valves on each solvent line ensure that solvent is drawn from the solvent bottles and ejected to waste without switching valve positions Table 33 lists the tubing requirements and identifies the tubing components shown in Figure 260 The Low Pressure Solvent Line Kit P N LC230 contains the Tees check valves and low pressure solvent lines to connect one pump A or B to the waste system and to its corresponding solvent bottle and six port rotary valve The EASY nLC 1000 Flow Line Kit P N LC570 contains all of these flow lines The stainless steel tubing is prebent Table 33 Tubing requirements for the EASY nLC 1000 system No Connections Tubing Part number 1 Pump outlet to pressure sensor inlet Stainless steel 250 um ID 150 mm length LC512 2 Pressure sensor outlet to valve A or B Stainless steel 250 um ID 150 mm length LC513 3 Pressure sensor outlet to valve S Stainless steel 250 um ID 150 mm length LC514 4 Mixing Tee to valve S nanoViper 20 um ID 350 mm length LC522 valve A to flow sensor A valve B to flow sensor B flow sensor A to mixing Tee 5 Flow sensor B to mixing Tee nanoViper 10 pm ID 180 mm length LC543 6 Autosam
30. Thermo Home Batch Setup Method Editor Maintenance Configuration SCIENTIFIC Overview Pump A Graphs Desired flow Real flow Pressure Queue ea aunssasg E gt Job 00 29 20 Sample 00 29 20 Gradient 20 00 18 jul 2011 12 01 admin a G A 125 150 175 200 225 25 0 Exit Time min The pressure starts to drop at approximately 25 minutes as 100 solvent B reaches the column 176 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific Thermo Scientific 6 Troubleshooting Troubleshooting Tips Figure 184 Pressure dip begins later when the system has introduced swept volume Thermo fom Batch Setup Method Editor Maintenance Configuration SCIENTIFIC Overview Pump A Queue Desired flow Real flow Pressure z E gt D F S Job 00 29 45 Sample 00 29 45 Gradiem 20 00 18 Jul 2011 13 34 admin oo 25 SO 75 100 125 150 175 200 225 Exit Time min The pressure starts to drop at approximately 27 minutes as 100 solvent B reaches the column Back pressure is proportional to the viscosity of the solvent mixture If you are not running a water acetonitrile gradient the pressure profile of your gradient run will differ Table 31 on page 311 lists the viscosities for two solvent mobile phases consisting of a water methanol mixture or a water acetonitrile mixture EASY nLC Series Troubleshooting
31. Thermo Scientific 4 Routine Maintenance Replacing a Flow Sensor To install an EASY nLC II flow sensor Install the new flow sensor into the bracket as follows a Align the new flow sensor with the mounting holes in the bracket IMPORTANT You must replace flow sensor A with type SLG1430 150 and flow sensor B with type SLG1430 025 b Insert the two screws that you removed earlier in step 6 of To remove an EASY nLC II flow sensor into the bracket see Figure 90 on page 98 c Using a 2 5 mm L hex wrench tighten the two screws to secure the flow sensor to the bracket Reconnect the cable to the flow sensor and tighten the metal ring see Figure 89 on page 98 Reconnect the solvent lines to both ends of the flow sensor see Figure 88 on page 97 Remount the bracket to the panel as follows a Align the bracket with the mounting holes in the panel b Insert the screws that you removed from the bracket when you removed it from the panel c Using a 2 5 mm L hex wrench tighten the two screws to secure the flow sensor bracket to the panel see Figure 87 on page 97 Mount the right side panel to the instrument housing Then with a 2 Phillips head screwdriver secure the panel by rotating the three captive screws a quarter turn clockwise Turn on the EASY nLC II instrument Wait 30 minutes for the flow sensors to reach the proper operating temperature Calibrate the new flow sensor by
32. To change the position of a valve 1 On the Home gt Overview page press the valve icon for the valve that you want to control The Valve dialog box opens see Figure 207 The readback box lists the current valve position Figure 207 Valve dialog box Valve A Valve A E e gp F l 1 2 I Center J 1 6 J 4 2 Readback box c 2 Press the button for the position that you want the valve to switch to 1 2 Center or 1 6 The readback box displays the new position 204 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 6 Troubleshooting Using the Direct Controls for Troubleshooting and Maintenance Using the Pump Controls You can refill and empty the pumps from the Home gt Overview page IMPORTANT Use the pump s direct controls for troubleshooting To avoid contaminating the pumps take care when you use these controls e For pump A or B make sure that its corresponding valve is in position 1 2 before you refill the pump e For pump S before you empty or fill the pump use the autosampler s direct controls to insert the autosampler needle into the W4 or W3 bottle respectively Tip To prepare the EASY nLC instrument for operation use the Prepare Purge Solvent script to flush the solvent lines see Prepare Purge Solvent on page 32 The Purge Solvent script places the valves in the appropriate positions for filling and empt
33. To check if you must replace the solvent line that connects the outlet of flow sensor A to the mixing Tee do the following a Set valve A to the Center position Disconnect the solvent line from the inline filter that connects to the outlet of flow sensor A Then block the inline filter with a blind fitting Figure 225 shows the solvent path blocked at the outlet of flow sensor A c Reset valve A to position 1 6 Figure 225 Solvent A channel blocked at the outlet of flow sensor A Valve W Valve A Valve B If the flow is lt 100 nL min with this line removed from the solvent path install a new solvent line Outlet of flow sensor A plugged with a blind fitting Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 241 6 Troubleshooting Troubleshooting the Results of the System Leak Test d Check the readback for flow sensor A Then depending on the flow rate do one of the following e If the flow rate is lt 100 nL min do the following i Stop the Analytical Column Equilibration script ii Disconnect the damaged solvent line from the mixing Tee s inlet for the solvent A channel iii Install a new solvent line between the mixing Tee s inlet for the solvent A channel and the flow sensor s outlet For ordering information see Table 33 on page 316 iv Reconnect the solvent lines and rerun the system leak test see
34. a Make sure that a wash bottle with a needle wash insert is installed in position W4 Send the autosampler needle to the appropriate position as follows On the Home gt Overview page press the autosampler icon The Autosampler dialog box opens see Figure 210 on page 210 Press W4 The autosampler needle moves to the W4 position EASY nLC Series Troubleshooting and Maintenance Guide 209 6 Troubleshooting Using the Direct Controls for Troubleshooting and Maintenance c Press Needle Down The autosampler needle descends into the W4 bottle d Press Close to close the Autosampler dialog box 3 Dispense solvent from pump S as follows a Press the pump S icon The Pump S dialog box opens b In the flow rate box enter the flow rate 0 01 to 300 pL min c Inthe volume box enter the volume that you want the pump to empty to waste d Press Start The pump empties until it pumps the requested volume or reaches the empty position whichever occurs first Using the Autosampler Controls You can control the XYZ robot and the cooler temperature from the Home gt Overview page To open the Autosampler dialog box Press the autosampler icon on the Home gt Overview page The Autosampler dialog box opens see Figure 210 Figure 210 Autosampler dialog box Autosampler XYZ Robot Pas gt Eject Plate Insert Plate J ne 8x12 Abgene AB 0800 Goto Calibration F Vial or
35. install the components supplied in the Pump Mounting Kit as described in To install the bracket and adapter plate provided in the Pump Mounting Kit on page 160 Then continue this installation at step 9 158 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 8 9 10 11 12 13 14 15 16 Thermo Scientific 5 Field Service Maintenance Replacing a Pump Connect the new pump as follows a Using a3 mm ball driver or L hex wrench and the four 3 mm hex screws that you set aside when you removed the damaged pump from the instrument secure the new pump to the instrument panel b Connect the pressure sensor cable from the new pressure sensor to the receptacle on the right side of the PLU pump c Connect the P Bus communication cables Check the address setting for the rotary switch If necessary use a small flathead screwdriver to change the rotary switch address on the new pump PCB e Pump A 1l ID e Pump B 2 997 0 e PumpS 3 y w Ig Install tubing from the pump to the pressure sensor and from the pressure sensor to the valve Use PEEK tubing for subsystems A and S Use stainless steel tubing for subsystem B Reconnect the power cable to the instrument s back panel and turn on the EASY nLC II instrument Prime the new pump as described in Priming the Pump on page 71 Remove air from the system as described in Removing Air After Replacing a Piston Seal or a
36. kod To prepare the autosampler for the plate calibration routine 1 Install the appropriate adapter plate 2 To calibrate the vial or well positions on a plate do one of the following e To calibrate the 6 x 8 vial positions remove the vial caps from two vials and load them into the adapter plate in positions Al and F8 e To calibrate a microwell plate remove the mat or cover from the microwell plate and load the microwell plate onto the adapter plate 3 Open the Tools view for the autosampler as follows a Press Maintenance gt Devices b In the Devices list select the autosampler c Press the Tools tab 4 In the Plate area do the following a Select the appropriate plate format from the list or create a new plate format To create and select plate formats see Managing Plate Formats on page 274 b Press Use 5 Remove the left side panel so that you can watch the movement of the needle To calibrate the plate go to the next topic Calibrating Plates To prepare the autosampler for the V1 and W4 calibration routine 1 Place an empty vial in position V1 The V1 position is not available for the microtiter plate formates 2 Prepare the bottle for position W4 used for ejecting waste and cleaning the outside of the injection needle 3 For information about preparing the W4 wash bottle refer to the EASY nLC Series Getting Started Guide 278 EASY nLC Series Troubleshooting and Maintenance Gu
37. see Figure 129 on page 132 9 Remove the ASA autosampler through the side of the housing 10 Disconnect the cables that supply power to the fans and remove the fan assembly at the back of the housing see Figure 124 Figure 124 Removing the fan assembly and cables Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 129 5 Field Service Maintenance Upgrading from an ASA Autosampler to an ASC Autosampler To install the ASC autosampler into an instrument that previously held the ASA model Note Because you are upgrading the instrument by adding an ASC autosampler this upgrade procedure differs from the simple installation procedure on page 127 1 As you insert the new autosampler into the housing move the loosened valve W a little to create the required space as shown in Figure 125 Figure 125 Putting in the ASC autosampler 2 Align the holes in the autosampler plate with the three mounting holes in the bottom of the housing see Figure 126 Figure 126 Aligning the holes in the autosampler plate with the mounting holes 130 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 5 Field Service Maintenance Upgrading from an ASA Autosampler to an ASC Autosampler 3 Reinstall the computer box into the EASY nLC housing 4 Reconnect all of the internal cables to the computer box Figure 98 on page 109 shows the connections to the computer box inside the EASY nLC II
38. take care when loading samples into the instrument s tray compartment WARNING SHARP NEEDLE AND MOVING PARTS i KEEP HANDS CLEAR AVERTISSEMENT AIGUILLE POINTURE ET PARTIES MOUVANTES TENIR LES MAINSALECART This sticker alerts you to consult this manual for instructions on how to operate the instrument The safety and special notices in the documentation include the following CAUTION Highlights hazards to humans property or the environment Each CAUTION notice is accompanied by an appropriate CAUTION symbol CAUTION Highlights electrical hazards to humans or property Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide xxi Preface CAUTION Highlights a sharp object hazard to humans CAUTION Highlights an eye hazard to humans CAUTION Highlights a chemical hazard to humans property or the environment CAUTION Highlights lifting hazards gt p gt e IMPORTANT Highlights information necessary to prevent damage to software loss of data or invalid test results or might contain information that is critical for optimal performance of the system Note Highlights information of general interest Tip Highlights helpful information that can make a task easier xxii EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific Preface Contacting Us There are several ways to contact Thermo Fisher Scientific for the information you need To contact Technical
39. 1000 instrument most of the plumbing connections use nanoViper fittings see Figure 1 Figure 1 nanoViper fitting SS PEEK sealing Screw surface Removable knurled nut Even though nanoViper fittings can withstand UHPLC back pressures of up to 1034 bar 15 000 psi they are fingertight fittings which require only very small torques to seal To avoid damage by overtightening follow the instructions in this guide Contents e Connecting nanoViper Fittings e nanoViper Tubing Connections e Returning the System to Atmospheric Pressure Connecting To connect a nanoViper fitting to a receiving port nanoViper Fitti ngs 2 Using the black knurled nut turn the screw clockwise to an angle between 0 and 45 degrees 1 8 turn 3 Run the system leak test see Running a System Leak Test on page 218 Insert the nanoViper fitting into the receiving port Then turn the screw clockwise until you feel resistance When the leak test ends the system is at atmospheric pressure IMPORTANT To extend the lifetime of the nanoViper fittings open and close connections at atmospheric system pressures only Opening and closing connections at high system pressures can reduce the lifetime of the fitting system 4 If the Leaks script fails because the new connection is not leak tight use the black knurled nut to turn the screw up to an additional 45 degrees Do not turn the screw beyond an angle of 90 degrees from where
40. 128 CAUTION Wear powder free gloves and safety glasses when handling parts of the LC system that come into contact with solvents Replacing the ASC autosampler requires these items Tools Parts and materials e 2 Phillips screwdriver e Powder free gloves 3 mm L hex wrench or ball driver e in or 10 cm length tie wrap e Torx T 10 wrench e Autosampler ASC P N LC301 e Cutting pliers To replace the autosampler follow these steps 1 To remove the ASC autosampler on this page 2 To install the ACS autosampler into an instrument that previously included this model on page 127 To remove the ASC autosampler 1 Remove the old autosampler from the device list as follows a Press Maintenance gt Devices b Select the autosampler in the Devices list c Press Remove Device The Remove Device dialog box opens d Press Accept 1 Close down the EASY nLC instrument see Closing Down the EASY nLC Instrument on page 21 Then unplug the power cable 2 Using a 2 Phillips head screwdriver with a quarter turn loosen the three captive quarter turn screws that secure each side panel to the instrument housing Then remove both panels 122 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 5 Field Service Maintenance Replacing the ASC Autosampler 3 Remove the computer box from the instrument as described in To remove the computer box on page 108 4 Unscrew the autosampler needl
41. 41 108 93 port 22 Figure 250 Ethernet connection between the EASY nLC system and the laboratory LAN port LAN port with Internet capability 288 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific Thermo Scientific 2 If you have not already set up the support server configuration set it up as follows a 8 Remote Support Connecting the EASY nLC Instrument to the Support Server Press Configuration gt Network The Network page of the Configuration menu opens Figure 251 Figure 251 Network page of the Configuration menu Home Batch Setup Method Editor Maintenance SCIENTIFIC Users LAN Connections Profile Internet Configuration Dynamic Fixed Network IP 10 32 134 69 Subnet 255 255 255 Gateway 10 321 134 254 DNS 10 32 135 Name Ic 009808 Domain Apply Support server Job 00 00 35 Sample 00 00 24 IP 195 0 41 0 108 93 f Default IP Gradient 00 00 Apply 5 Mar 2012 16 27 admin Exit In the LAN area do the following i Select Internet in the Profile list ii Press Apply In the Support Server area do the following i Enter the following IP address 195 41 108 93 ii Pres
42. 53 Keeping Service Records o 2 acccasR aches x wcatavended mai iia iea ia aaia Ge aat 55 Checking and Resetting the Device Usage Counters 0 0 00005 56 Chapter4 Routine Maintenance 0c cece eee eee eee eee eee 57 Maintaining a Clean Working Environment 0 00000 eee 58 Replacing the Main Power Fuse 50 2 09 ios oot e PAO n eee es 58 Maintaining the Syringe Painps ines Gat dates eine a urere 60 Retracting the Piston ca eater a aioe raeey hates aa 61 Replacing the Piston Seal and Cleaning the Piston ina PLF Pump 62 Replacing the Piston Seal in a PLU Pumps cc 0 2s cee ee eee eee 67 Priming the Puget trates tonite etai aea kia i iae eE acid 71 Resetting the Pump Usage Counter n 545ib oe wes dokaeei sae nee 72 Removing Air After Replacing a Piston Seal or a Pump 73 Running the Leaks Script after Replacing a Piston Seal or a Pump 73 Maintaining the Rotary Valves io 0 5s teis deo aan aan nce Bed eases de ea 74 Cleaning the Rotor Seal and Stator eis bi wtoP ts Bae tate heis aless 74 Replacing the Rotor Seah socks Wp eben eh Sa aay Z7 Replacing the Staton uit oea eiir eaae eana a i eana i iiye 78 Replacing the Check Valves t 5 ivwid caegee beans oe ruuna 80 Replacing an Inline Filter for the EASY nLC 1000 Instrument 81 Using nanoViper Fittings 0 0 cee eee 83 Replacing the Autosampler Needle 0 2 eee cee eee 84 Replacing the Sample Loops af
43. 7 Calibrating the RETURN_CODE for asr codes Autosampler s XYZ Robot 59 ERROR_ Vial configuration change Error while saving Calibrated position not MANIPULATOR_SET_ failed autosampler vial recognized by software See VIAL_CONFIG AUTOSAMPLER_RET calibration see code 57 Chapter 7 Calibrating the URN_CODE for asr codes Autosampler s XYZ Robot 306 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific Table 30 Error codes Sheet 5 of 8 A Error Codes No Issue code name Description Cause Solution 60 ERROR_ tray eject failed see code 57 for asr Verify that the correct plate MANIPULATOR_TRAY AUTOSAMPLER RET codes is configured in the _EJECT URN_CODE EASY nLC software and the Xcalibur VI Check that no objects in the tray compartment are obstructing the XYZ robot movement Reset manipulator and run the Torque script for the ASC model autosampler 61 ERROR_ tray load failed see code 57 for asr see above MANIPULATOR_TRAY AUTOSAMPLER_RET codes _LOAD URN_CODE 62 ERROR_ grid address failed Could not move to see above MANIPULATOR_GRID AUTOSAMPLER_RET sample position see _ADDRESS URN_CODE code 57 for asr codes 63 ERROR_ wash address failed Could not move to see above MANIPULATOR_ AUTOSAMPLER_RET wash position see code WASH_ADDRESS URN_CODE 57 for asr codes 64 ERROR_ vial address failed Could not move to vial see above MANIPULATOR_VIAL_ AUTOSAMPLER_RET position see
44. A and B select System Figure 35 on page 45 shows the pressurized solvent path for the system leak test 3 For a system leak test connect the Column Out line to the Waste In line using the union supplied in the accessory kit as shown in Figure 35 on page 45 Instrument Part number of the stainless steel union EASY nLC II SC600 EASY nLC 1000 SC900 For information about troubleshooting system leaks see Troubleshooting the Results of the System Leak Test on page 221 and Troubleshooting a Pump that Fails the Leaks Script on page 216 46 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 3 Maintenance Scripts and Service Records Maintenance Scripts Test Valve Check The Valve Check script performs a self test for the selected valves of an EASY nLC II instrument Use this script when valves begin to move slowly or cannot reach the required positions and after exchanging valve parts rotor or stator IMPORTANT This script is only valid for EASY nLC II valves with a serial number less than V 009999 Make sure to record any numbers that the script generates If the overshoot is greater than 6 contact your local Thermo Fisher Scientific field service engineer Figure 36 shows the parameters for the Valve Check script Figure 36 Valve Check script parameters Thermo Home Batch Setup Micidgeemacem Maintenance Eedse aeg SCIENTIFIC Category Name f f f No graph X Log Book Test
45. Edisonsvej 4 DK 5000 Odense Denmark Laboratory Instrumentation Model Name EASY nLC 1000 Model Numbers LC120 Serial Number LC 010000 and higher Year of Manufacture 2011 I the undersigned hereby declare that the equipment specified above conforms to the above Directive s and Standard s g Alo Ole Vorm Site Manager July 19 2011 WEEE Compliance This product is required to comply with the European Union s Waste Electrical amp Electronic Equipment WEEE Directive 2002 96 EC It is marked with the following symbol Thermo Fisher Scientific has contracted with one or more recycling or disposal companies in each European Union EU Member State and these companies should dispose of or recycle this product See www thermoscientific com rohsweee for further information on Thermo Fisher Scientific s compliance with these Directives and the recyclers in your country WEEE Konformitat Dieses Produkt muss die EU Waste Electrical amp Electronic Equipment WEEE Richtlinie 2002 96 EC erf llen Das Produkt ist durch folgendes Symbol gekennzeichnet Thermo Fisher Scientific hat Vereinbarungen mit Verwertungs Entsorgungsfirmen in allen EU Mitgliedsstaaten getroffen damit dieses Produkt durch diese Firmen wiederverwertet oder entsorgt werden kann Mehr Information ber die Einhaltung dieser Anweisungen durch Thermo Fisher Scientific ber die Verwerter und weitere Hinweise die n tzlich sind um
46. Environment Maintain a clean working environment for the EASY nLC instrument When cleaning the outside of the EASY nLC instrument use a mild detergent and a clean cloth Replacing the Main Power Fuse The EASY nLC uses these fuses For 120 Vac the instrument uses one T 5 AL 250 V fuse 5 x 20 mm IEC 60127 e For 230 Vac the instrument uses one T 2 5 AL 250 V fuse Use only UL Listed and CSA certified fuses All fuses supplied with the instrument are UL Listed and CSA certified CAUTION Before removing the fuses turn off the instrument and remove the power cable To replace the main power fuses 1 Move the EASY nLC instrument to a benchtop where you can access the back panel Figure 45 shows the steps required to remove the fuse holder 2 Turn off the power and pull out the power plug 3 Place a flat blade screwdriver into the slot in the top of the fuse holder and then turn the screwdriver to loosen the fuse holder 4 Pull the fuse holder out of the power entry module Figure 45 Replacing the main power fuse D a DS Turn off the power and pull the power plug Using a flat blade screwdriver loosen the out of the power receptacle fuse holder 58 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 4 Routine Maintenance Replacing the Main Power Fuse 5 To install the appropriate fuse do one of the following e Insert the 5 A fuse into the slot that aligns with the white triangle at
47. For information about running the system leak test see Running a System Leak Test on page 218 Note You cannot perform the leak test for pump S as it does not operate under pressure during normal batch execution Figure 34 shows the parameters for the Leaks script Figure 34 Leaks script parameters Thermo Home Batch Setup Micidgeemashiiiem Maintenance Configuration SCIENTIFIC wae og Book Support Devices fro graph Job 00 15 48 11 May 2011 admin Exit During the leak test of pump A or B the following events occur Step Event 1 The pump refills 2 The associated valve centers blocking the solvent flow through the valve 3 The pump builds pressure to 280 bar for the EASY nLC II instrument or 980 bar for the EASY nLC 1000 instrument 4 When the pressure stabilizes at 280 bar for the EASY nLC II instrument or 980 bar for the EASY nLC 1000 instrument the pump calculates the flow loss based on the pump piston movement required to maintain this pressure If the flow loss is less than 400 nL min the pump passes the leak test 44 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 3 Maintenance Scripts and Service Records Maintenance Scripts During a system leak test Leaks script with System selected the script pressurizes the flow paths shown with red dashed lines in Figure 35 monitors the flow sensors and pump piston moveme
48. Maintenance Guide 77 4 Routine Maintenance Maintaining the Rotary Valves 6 Using a 9 64 in L hex wrench tighten the two hex screws a little at a time by shifting from one screw to the other and back again until the screws are evenly torqued 7 Mount the right side panel to the instrument housing Then with a 2 Phillips head screwdriver secure the panel by rotating the three captive screws a quarter turn clockwise To reset the usage counter for the valve 1 Turn on the EASY nLC instrument and log in to the system as an administrator 2 Reset the usage counter for the valve as follows a Press Maintenance gt Devices b Select the valve from the list of devices c Press the Summary tab d Press Reset The value in the Rotor Shifts box resets to 0 For more information see Checking and Resetting the Device Usage Counters on page 56 3 To remove air from the system and check for leaks follow To remove air from the system and check for leaks after disassembling a valve on page 76 Replacing the Stator Replacing the stator requires one of these parts Valve serial number Replacement part V 001000 V 099999 Coated stator P N LC226 V 000100 V 000999 Uncoated stator P N LC225 Tip The About page of the Rotary Valve view on the Maintenance gt Devices page lists the serial number of the selected valve To replace the stator in a valve and return the instrument to normal operation follow these step
49. Maintenance Guide Thermo Scientific 6 Troubleshooting Troubleshooting the Results of the System Leak Test EASY nLC 1000 Locating a Leak Between the Flow Sensors and Valve S To determine whether the leak is coming from the connection to port 4 of valve S the connection to the mixing Tee outlet or the connections between the flow sensor outlets and the mixing Tee inlets follow the workflow in Figure 221 or the procedure on pages 236 239 Figure 221 EASY nLC 1000 workflow for locating leaks between the flow sensors and valve S System leak test fails and returns the following message Check solvent lines between flow sensor A and valve S including the mixing Tee and flow sensor B for leaks Redo the nanoViper fitting connection to port 4 of valve S step 1a on page 236 Start the Analytical Column Equilibration script step 1b on page 236 Readback for flow sensor A gt 100 nL min Disconnect the nanoViper fitting from the mixing Tee outlet Then block the mixing Tee outlet with a blind fitting step 2 on page 236 Install a new solvent line between port 4 of valve S and the mixing Tee outlet step 2d on page 238 Readback for flow sensor A gt 100 nL min Then reconnect the plumbing and rerun the system leak test page 218 Disconnect the blind fitting from the mixing Tee outlet and then reconnect the solvent line to the mixing Tee outlet Disconnect the solvent line from the mixing Tee
50. OUT3 IN2 OUT2 IN1 OUT1 OE DRIVE This device complies with Part 15 of the FCC Rules Operation is subject to the following two conditions 1 This device may not cause harmful interference and 2 This device must accept any interference received including interference that may cause undersired operation This instrument is for research use only DEVICE EASY NLC AAOZL OLL P N LC100 LAN MAC ADDRESS 00 6 E0 45 4E 28 220 240 V Proxeon Biosystems A S C 120 230 V 50 60 Hz 250 W Web www proxeon com y Fuse at 120 V T5 AL 250 V Phone 45 6557 2300 Fax 45 6557 2301 aig S Ee S eA Figure 17 Back panel of the EASY nLC instrument with the latest computer box a gt OA oUo do IN3 OUT3 IN2 OUT2 IN1 OUT1 Cae DRIVE This device complies with Part 15 of the FCC Rules Operation is subject to the following two conditions 1 This device may not cause harmful interference and 2 This device must accept any interference received including interference that may cause undersired operation This instrument is for research use only DEVICE EASY NLC AAOZL OLL P N LC100 LAN MAC ADDRESS 00 6 E0 45 4E 28 220 2400 Thermo Fisher Scientific A S 120 230 V 50 60 Hz 250 W Web www proxeon com zg Fuse at 120 V T5 AL 250 V Phone 45 6557 2300 Fax 45 6557 2301 SA EE EN 16 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific Thermo Scientific 1 Introduction Hardware Components Tab
51. P BUS interface in service Trying to initialize the Check that the RS485 SERVICE_MODE mode RS485 interface while switch on PC is in the serial port is in the ON position service mode 28 ERROR PBUS_ Initialization of P BUS see issue description Troubleshoot P BUS INTERFACE_INIT interface failed communication Check that the cables are properly connected 29 ERROR PBUS_ Error writing to interface see issue description Troubleshoot P BUS SEND_BYTES COMM_PORT communication Check that the cables are properly connected 30 ERROR PBUS_ Error reading from see issue description Troubleshoot P BUS READ_BYTES interface COMM_PORT communication Check that the cables are properly connected 31 ERROR_SENSIRION_ Failed parsing flow sensor Could not parse some Cycle EASY nLC power FACTOR_PARSING factor from string to a double INFO_COMMAND 32 ERROR_SENSIRION_ Flow sensor factor not Error getting flow Cycle EASY nLC power FACTOR_NOT_ found in info output sensor scaling factor FOUND Factor unknown 304 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific Table 30 Error codes Sheet 3 of 8 A Error Codes No Issue code name Description Cause Solution 33 ERROR_ Port COM_PORT does Error getting input Cycle EASY nLC power SENSIRION_ not support reading Error from flow sensor INIT getting input stream from flow sensor port COM_PORT 34 ERROR SENSIRION_ No data received from see issue descript
52. Pump on page 73 Make sure that both the right and left instrument panels have been installed and that the instrument has been turned on for at least 30 minutes Run the flow sensor calibration for the new pump see Calibrate Flow Sensors on page 51 If you replaced pump S run the Sample Pickup script see Test Sample Pickup on page 42 The instrument is now ready for use EASY nLC Series Troubleshooting and Maintenance Guide 159 5 Field Service Maintenance Replacing a Pump To install the bracket and adapter plate provided in the Pump Mounting Kit Note Some earlier versions of the EASY nLC II instrument cannot accommodate a PLU pump in the pump S position without modification To provide space for the pump outlet tubing in these instruments you must install the new flow sensor bracket and the pump adapter plate supplied in the Pump Mounting Kit The new flow sensor bracket places the flow sensors in a higher position and the pump adapter plate places the pump in a lower position than their original positions on the instrument panel This repositioning makes space for the tubing that is connected to the pump head 1 If you have not already done so follow step 1 on page 154 through step 6 on page 158 of the previous procedure To replace a PLF pump with a PLU pump in the EASY nLC II instrument to upgrade the touch screen software gain access to the solvent system compartment remove the dam
53. Running a System Leak Test on page 218 e Ifthe flow rate is gt 100 nL min go to the next step 2 To check the connection between the inline filter and the flow sensor A outlet do the following a Using a 5 16 in open ended wrench tighten the inline filter to the outlet of flow sensor A b Check the readback for flow sensor A Then depending on the flow rate do one of the following e If the flow rate is lt 100 nL min do the following i Stop the Analytical Column Equilibration script ii Reconnect all of the solvent lines iii Rerun the system leak test see Running a System Leak Test on page 218 e Ifthe flow rate is gt 100 nL min do the following i Stop the Analytical Column Equilibration script ii Remove the damaged inline filter and install a new inline filter iii Rerun the system leak test see Running a System Leak Test on page 218 242 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 6 Troubleshooting Troubleshooting the Results of the System Leak Test EASY nLC 1000 Locating a Leak Between Flow Sensor B and the Mixing Tee By following the workflow in Figure 221 on page 235 or the procedure on pages 236 239 you determined that the leaking connection is between the flow sensor B outlet and the mixing Tee inlet The system plumbing is set up as shown in Figure 223 on page 239 with the solvent channel B disconnected from the mixing Tee inlet Foll
54. Select the check boxes for the appropriate pump or pumps 3 Press Start 32 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 3 Maintenance Scripts and Service Records Maintenance Scripts Prepare Flush Air The Flush Air script removes air from inside the pump head by pressurizing the pump and then releasing that pressure into the flow path toward the waste beaker The pump refills pressurizes releases pressure and empties for each iteration of the script While the instrument executes the script the software calculates the pressurization time dynamically based on measurements from previous iterations and measures the pumped volume required to reach 200 bar Note Because the solvents are slightly compressible and the pump itself expands even an air free pump allows some pumping before reaching 200 bar Depending on the solvents a pumped volume less than 10 uL for the PLF pump model or 12 pL for the PLU pump model is acceptable Note Figure 8 on page 9 shows the PLF and PLU model pumps While the PLU model pump is standard in EASY nLC 1000 instruments and the PLF model pump is standard in most EASY nLC II instruments the PLU model pump is the replacement part for both instrument models Figure 25 shows the parameters for the Flush Air script Figure 25 Flush Air script parameters Thermo Home Batch Setup Wicidgeemactieem Maintenance Eerie Elgea SCIENTIFIC ripts Category Name
55. Stator on page 78 Maintaining the valves by cleaning the rotor seal and stator or by replacing the rotor seal the stator or both parts requires these tools and materials Tools Materials e 9 64 in hex wrench e Powder free safety gloves e 1 4 in open ended wrench e LC MS grade methanol e 2 Phillips head screwdriver e Lint free cloth CAUTION Wear powder free gloves and safety glasses when handling parts of the LC system that come into contact with solvents Cleaning the Rotor Seal and Stator If a rotary valve is leaking attempt to remove the leak by cleaning the rotor seal and the stator before taking the more costly step of replacing the rotor seal To clean the rotor seal and stator in a valve and return the instrument to normal operation follow these steps 1 To remove the stator from the valve on page 75 2 To clean the rotor seal and the stator on page 75 3 To reassemble the cleaned valve and return the instrument to normal operation on page 76 74 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific Thermo Scientific KD kod 1 3 4 Routine Maintenance Maintaining the Rotary Valves To remove the stator from the valve Close down the EASY nLC instrument and then turn off the power to the instrument see Closing Down the EASY nLC Instrument on page 21 Using a 2 Phillips head screwdriver with a quarter turn loosen the three captive screws that secur
56. Thermo Scientific A Remote Support The EASY nLC instrument includes a remote support feature that enables Thermo Fisher Scientific Technical Support to diagnose and troubleshoot your EASY nLC system remotely while the instrument is connected to the support server When you connect the instrument to the remote server you can also install the latest firmware file for the touch screen application Technical Support cannot initiate the communication link between your EASY nLC instrument and the support server You must initiate this communication link from your EASY nLC instrument To connect the EASY nLC instrument to the support server or download system files to a removable storage device follow these procedures Contents e Connecting the EASY nLC Instrument to the Support Server e Saving System Files on a USB Removable Storage Device e Downloading the Latest Firmware File IMPORTANT To establish communication with the support server you must first connect the EASY nLC instrument directly to your local network and then open the communication link for remote support through the touch screen software Thermo Fisher Scientific Technical Support cannot initiate this communication link The EASY nLC instrument uses Secure Shell SSH a network protocol for secure data communication to connect to the support server which uses the Linux operating system To use the remote support feature the firewalls for your local netw
57. To replace a pump follow the appropriate procedure e Replacing a PLU Pump on this page e Replacing a PLF Pump with a PLU Pump on page 154 CAUTION Wear powder free gloves and safety glasses when handling parts of the LC system that come into contact with solvents Replacing a PLU Pump Replacing a PLU model pump requires these tools and materials Tools Parts and materials e 2 Phillips screwdriver e Powder free gloves e 1 4 in open ended wrench e PLU model pump P N LC501 e 2 5 mm hex wrench e 3 mm hex wrench Small flathead screwdriver e Pipette for priming the pump To replace a pump in the EASY nLC instrument follow these steps 1 To remove a PLU model pump from an EASY nLC instrument on page 151 2 To install a PLU pump in an EASY nLC instrument on page 153 150 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific Thermo Scientific 5 Field Service Maintenance Replacing a Pump To remove a PLU model pump from an EASY nLC instrument Set the valve for the pump to position 1 6 as follows a Press Home gt Overview b Press the appropriate valve icon c In the Valve dialog box press Center Placing the valve in the Center position shuts off the solvent flow to the subsystem Close down and then turn off the EASY nLC instrument Disconnect the power cable from the instrument s back panel Using a 2 Phillips head screwdriver with a quarter turn loo
58. Using the piston seal tool to remove the worn piston seal Worn piston seal Installing a New Piston Seal 1 Insert the guide tube into the pump head flange Figure 6 Guide tool inserted into the pump head flange Guide tool 2 Using a pipette fill the groove in the piston seal with methanol 3 Place the new seal with the groove facing up on the piston seal tool Figure 7 Piston seal mounted onto the piston seal tool 4 Insert the piston seal tool into the guide tool Figure 8 Inserting the piston seal tool into the guide 5 Push the piston seal tool into the guide until you feel resistance 6 Remove the guide tube and the piston seal tool and then check that the piston seal is properly seated 7 Using a 13 mm open ended wrench reconnect the pump head to the pump body Do not connect the stainless steel tubing to the pump head 330 Priming the Pump 1 Turn on the EASY nLC instrument and log in as an administrator 2 Fill the pump head with the appropriate solvent as follows a Press Home gt Overview b Press the pump icon for the pump that you want to control c Set the flow rate to 300 p L min and the volume to 140 pL Then press Start The piston moves upward to the top of the pump head d Pipette the appropriate solvent into the pump head e Set the flow rate to 300 pL min and the volume to 140 pL Then press Start The piston moves down drawing solvent into the pump head M
59. a pump PCB from the PLU model pump on this page 2 To replace a pump PCB on the PLU model pump on page 166 To remove a pump PCB from the PLU model pump 1 Close down the EASY nLC 1000 instrument as described in Closing Down the EASY nLC Instrument on page 21 2 Disconnect the power cable from the instrument s back panel 3 Using a 2 Phillips head screwdriver with a quarter turn loosen the three captive screws that secure the right side panel to the instrument housing Then remove the panel 4 Disconnect the P Bus communication cables the flow sensor cable and the pressure sensor cable from the pump Figure 176 on page 164 shows these cables connected to the pump Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 163 5 Field Service Maintenance Replacing a Pump PCB 5 Using a 2 5 mm L hex wrench or ball driver loosen the four captive screws on the PCB shield see Figure 176 Then remove the PCB shield see Figure 177 Figure 176 Four PCB shield screws PLU model pump Flow sensor cable Pressure sensor cable P Bus cables The four captive screws remain in place see Figure 177 Figure 177 Removing the PCB shield PLU model pump 164 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 5 Field Service Maintenance Replacing a Pump PCB 6 Slide the PCB forward and away from the four mounting studs 7 Disconnect the motor cable on the bac
60. and Maintenance Guide 177 6 Troubleshooting Troubleshooting Tips Excessive Duration or Higher Pressure for the Column Equilibration and Sample Loading Steps Table 13 lists possible causes for the following e When the column equilibration and sample loading steps are controlled by a specified pressure the duration of these steps is longer than expected When these steps are controlled by a specified flow the pressure is higher than expected during the column equilibration step the sample loading step or both steps During the sample loading and column equilibration steps pump A delivers the user specified volume of solvent A to the system at the user specified flow rate or maximum pressure or the maximum system pressure When the columns or the Column Out line is clogged the system must lower the flow rate to maintain the pressure at the maximum pressure specified in the method The lower flow rate increases the column equilibration time the sample loading time or both Table 13 Common causes of a longer than expected column equilibration time or sample loading time Symptom Possible cause Action When the method specifies a set Clogged column Run the Back Pressure script for pump A flow rate for the column see Test Back Pressure on page 48 equilibration sample loading or both of these steps the pressure is If the test passes one of the columns is higher than usual probably clogged and must be repla
61. and Service Records Maintenance Scripts Torun the Analytical Column Equilibration script 1 Open the Analytical Col Equilibration script as follows a b C Press Maintenance gt Scripts In the Category list select Prepare In the Name list select Analytical Col Equilibration abbreviated to fit in the space allotted 2 Set up the parameters for this script as follows a b Press the Parameters tab In the Volume pL box enter the volume of solvent A to use to equilibrate the analytical column For best results use at least 10 column volumes to equilibrate the analytical column In the Flow box pL min enter the flow rate for the analytical column equilibration step If the flow field is left empty the pump operates at the set pressure In the Max Pressure bar box enter the maximum allowed pressure If the pressure field is left empty the pump operates at the set flow as long as it is below the instrument maximum pressure of 300 bar for the EASY nLC II instrument or 1000 bar for the EASY nLC 1000 instrument If both a flow and a maximum pressure are specified the pump flow is limited by whichever parameter is reached first CAUTION Check the maximum pressure rating for the analytical column Running the instrument at pressures higher than the maximum pressure rating for the column reduces the column lifespan 3 Press Start EASY nLC Series Troubleshooting and Maintenance Guide 37
62. and a Thermo Scientific mass spectrometer Connect the two pin connector to the mass spectrometer s Start In pins Connect the 12 pin connector to the back panel of the EASY nLC instrument 2 Check the contact closure settings for the LC MS system as follows a On the EASY nLC touch screen press Configuration gt Connections b Fora Thermo Scientific mass spectrometer make the following selections In the Instrument cable no list select Thermo Scientific LC160 In the Protocol list under Contact Closure Settings select One way In the State at Start list under Contact Closure Settings select Open 3 Turn on the mass spectrometer and set it up to wait for a contact closure signal e Fora third party mass spectrometer follow the instructions in the documentation provided with the mass spectrometer Then go to step 5 e Foran LTQ Series mass spectrometer go to step 4 4 For an LTQ Series mass spectrometer set up the mass spectrometer to wait for a contact closure signal as follows a Open the Tune Plus window b On the menu bar choose Control gt Acquire Data Figure 31 shows the Control menu of the Tune Plus window for an LTQ Series mass spectrometer Figure 31 Control menu gt Thermo Tune Plus Untitled File View ScanMode Display Setup Diagnostics Help a y Standby ie a Tune Calibrate Acquire Data The Acquire Data dialog box opens see Figure 32 40 EASY
63. die Produkte zu identifizieren die unter diese ROHS Anweisung fallen finden sie unter www thermoscientific com rohsweee Thermo SCIENTIFIC Conformit DEEE Ce produit doit tre conforme a la directive europ enne 2002 96 EC des D chets d Equipements Electriques et Electroniques DEEE Il est marqu par le symbole suivant Thermo Fisher Scientific s est associ avec une ou plusieurs compagnies de recyclage dans chaque tat membre de Punion europ enne et ce produit devrait tre collect ou recycl par celles ci Davantage d informations sur la conformit de Thermo Fisher Scientific 4 ces directives les recycleurs dans votre pays et les informations sur les produits Thermo Fisher Scientific qui peuvent aider la d tection des substances sujettes la directive RoHS sont disponibles sur www thermoscientific com rohsweee CAUTION Symbol gt PPP e gt CAUTION Electric Shock This instrument uses high voltages that can cause personal injury Before servicing shut down the instrument and disconnect the instrument from line power Keep the top cover on while operating the instrument Do not remove protective covers from PCBs Chemical This instrument might contain hazardous chemicals Wear gloves when handling toxic carcinogenic mutagenic or corrosive or irritant chemicals Use approved containers and proper procedures to dispose waste oil Heat Before servicing the instrument allow any heated components to
64. e Ifthe pressure is above the threshold go to step 2 2 To determine if the Waste In line is blocked do the following a Disconnect the Column Out line and the Waste In line from the union lt a Column Out line Union Waste In line b Depending on the back pressure do one of the following Ifthe pressure is below the threshold the Waste In line is blocked Discontinue this troubleshooting procedure and replace the Waste In line with a new line Instrument Pressure threshold EASY nLC II 100 bar EASY nLC 1000 125 bar e Ifthe pressure is above the threshold go to step 3 3 To determine if the Column Out line is blocked do the following a Disconnect the Column Out line from port 3 of valve S b Depending on the back pressure do one of the following e If pressure is below the threshold the Column Out line is blocked Discontinue this troubleshooting procedure and replace the Column Out line with a new line Instrument Pressure threshold EASY nLC II 100 bar EASY nLC 1000 90 bar e Ifthe pressure is above the threshold reconnect the Column Out line and then go to step 4 Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 253 6 Troubleshooting Troubleshooting a System Blockage 4 To determine if valve S is partially or totally blocked do the following a Disconnect the mixing Tee line from port 4 of valve S b Depending on the back pressure do one of the following
65. e Radioactive Yes No e Toxic Yes No e Corrosive Yes No e Explosive Yes No Has the equipment been properly decontaminated and or cleaned before being returned Yes No Legally binding declaration I hereby declare that the information supplied on this form is accurate and sufficient to judge any contamination level Name Job title Organization Address Telephone Fax E mail Signature EASY nLC Series Troubleshooting and Maintenance Guide 301 ee i Error Codes Table 30 lists the possible error codes Table 30 Error codes Sheet 1 of 8 No Issue code name Description Cause Solution 1 ERROR_IO_CLOSING_ Failed closing log file File system error Internal filesystem error FILE Replace the hard drive 2 ERROR_IO_WRITING_ IOException I O error occurred Internal filesystem error TO_FILE Replace the hard drive 3 ERROR_IO_NO_FILE_ Log was closed trying to Filesystem error Internal filesystem error OPEN log the following message Replace hard drive 4 ERROR_IO_CREATE_ Failed creating log file I O error occurred Internal filesystem error FILE LOG_FILE_NAME Replace the hard drive 5 ERROR_IO_CREATE_ Failed creating log folder I O error occurred Internal filesystem error FOLDER LOG_FOLDER_NAM Replace the hard drive E 6 ERROR _JO_FILE_ Failed creating log Filesystem error Internal filesystem error EXISTS LOG_FILE_NAME Replace the hard drive AND
66. enter 12 pL 2 Do one of the following Ifthe Output page reports one of these messages go to Troubleshooting a Pump that Fails the Flush Air Script on page 212 Aborting unable to build pressure Aborting check solvent level e Ifthe script passes go to step 3 3 Run the Leaks script for the failed pump or both pumps see Test Leaks on page 44 Ifthe Leaks script fails the leak is either in the pump the valve or the connecting lines or fittings Go to step 4 Ifthe Leaks script passes excess air in the system was the source of the problem 216 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 6 Troubleshooting Troubleshooting a Pump that Fails the Leaks Script 4 To determine whether the leak is between the pressure sensor and the valve or the pressure sensor and the pump do the following a Remove the fitting from the top of the relevant pressure sensor and replace it with a blanking nut Tighten the nut with a 1 4 in open ended wrench e EASY nLC II blanking nut LC223 e EASY nLC 1000 blanking nut LC523 b Repeat the Test Leaks script c Depending on the result of the Test Leaks script do one of the following e Ifthe Test Leaks script passes go to step 5 as the piston seal is leak tight and the leak is between the pressure sensor and the valve Ifthe Test Leaks script fails go to step 6 as the leak is between the pressure sensor and
67. f 3 f No graph X Log Book Prepare Flushair Support beete Parametel Output Devices Parameter Flush pump A Flush pump B Flush pump Flush threshold pl Job 00 00 21 f No graph 11 May 2011 11 51 admin Schedule Exit Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 33 3 Maintenance Scripts and Service Records Maintenance Scripts Torun the Flush Air script 1 Open the Flush Air script as follows a Press Maintenance gt Scripts b In the Category list select Prepare c Inthe Name list select Flush Air 2 Set up the parameters for this script as follows a Press the Parameters tab b Inthe Flush Threshold pL box enter the acceptable threshold for the pump model For the PLF pump standard in most EASY nLC II instruments enter 10 For the PLU pump standard in the EASY nLC 1000 instrument enter 12 3 Press Start The script ends after the first iteration that gives a flush volume below the set threshold Prepare Precolumn Equilibration The Precolumn Equilibration script equilibrates the precolumn with solvent from pump A Use this script to equilibrate or flush the precolumn and to determine a suitable flow rate for the sample loading step in your method Figure 26 shows the parameters for the Precolumn Equilibration script Figure 26 Precolumn Equilibration script parameters Thermo Home Batch Set
68. for the EASY nLC 1000 instrument Sample loop Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 89 4 Routine Maintenance Replacing a Pressure Sensor for the PLU Pump 3 Connect the replacement sample loop to valve S as follows a Insert the fittings at the end of the sample loop into ports 2 and 5 of valve S b Depending on the instrument model secure the connections as follows e For the EASY nLC II instrument use a 1 4 in open ended wrench to tighten the fittings e For the EASY nLC 1000 instrument secure the fittings to their receiving ports as described in Using nanoViper Fittings on page 83 Take care not to overtighten these fingertight fittings 4 Check for leaks by running the system leak test as described in Checking the Sample Loop Connections for a Leak on page 222 Replacing a Pressure Sensor for the PLU Pump This topic describes how to replace a pressure sensor that is compatible with the PLU model pump standard in EASY nLC 1000 instruments Note For information about replacing a pressure sensor for a PLF model pump standard in most EASY nLC II instruments see Replacing a Pressure Sensor for a PLF Pump on page W7 Replacing the pressure sensor that is connected through solvent line tubing to a PLU model pump requires these tools and materials Tools Parts and materials 2 Phillips head screwdriver e Powder free safety gloves e 1 4 in open ended wrenc
69. for use Maintaining the Rotary Valves Quick Reference Guide Replace the rotor seal when you detect a leak or blockage in the valve Contents e Replacing a Rotor Seal e Replacing the Stator A Replacing a piston seal requires these tools and materials CAUTION Wear powder free safety gloves when working with solvents and handling the wetted components of the instrument Tools Materials 9 64 in hex wrench Rotor seal P N LC228 EASY nLC system valves with e 1 4 in open ended serial numbers V 010000 and wrench above e 2 Phillips head e Rotor seal P N LC224 screwdriver EASY nLC II system valves with serial numbers below V 009999 e LC MS grade methanol e Powder free safety gloves Replacing a Rotor Seal 1 Close down the EASY nLC system and then turn off the power to the instrument 2 Using a 2 Phillips screwdriver with a quarter turn loosen the three captive screws that secure the panel to the right side of the EASY nLC instrument and then remove the panel 3 Using a 9 64 in L hex wrench remove the two hex screws that secure the stator to the valve assembly and then pull the stator away from the valve driver 4 Remove the rotor seal from the valve driver Figure 1 Rotor seal mounted on the valve driver 5 Clean the stator with a lint free tissue or Q tip soaked in methanol Figure 2 Cleaning the stator 6 Carefully mount the new rotor onto the valve driver
70. high organic hold at the end of the run The gradient reaches 100 solvent B I hermo Home Baieide gest cb lemme v Eia aree M orice Maintenance Configuration Overview Pump A Graphs Desired flow Real flow Pressure ps 300 0 Queue 0 275 0 250 0 225 0 200 0 175 0 2 150 0 5 z 125 0 amp Job 00 29 20 100 0 Sample 00 29 20 75 0 Gradient 20 00 50 0 18 Jul 2011 12 01 25 0 admin 0 0 00 25 SO 75 100 125 150 175 200 225 250 275 30 0 Exit Time min The pressure begins to drop when the high organic mobile phase reaches the column EASY nLC Series Troubleshooting and Maintenance Guide 195 6 Troubleshooting Troubleshooting Tips Spray Issues Table 19 lists the possible causes of an unstable spray Table 19 Possible causes of an unstable spray Symptom Possible cause Unstable spray Post column outgassing Action Degas the solvents Poor connection in either the liquid junction or the DirectJunction Check all of the connections Incorrect MS settings or emitter position Optimize the MS tune file for the desired flow rate and the emitter position Dirty emitter Clean the emitter with an organic solvent such as methanol acetonitrile or ethanol Use only LC MS grade solvents Poor high voltage connection Check all of the high voltage connections Reconnect as necessary Air movement from an air conditioning vent 196 EASY nLC Se
71. inlet for the solvent B channel and then block the inlet with a blind fitting step 3 on page 238 Readback for flow sensor A gt 100 nL min No Yes Go to EASY nLC 1000 Locating a Leak Between Go to EASY nLC 1000 Locating a Leak Between Flow Sensor B and the Mixing Tee on page 243 Flow Sensor A and the Mixing Tee on page 240 Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 235 6 Troubleshooting Troubleshooting the Results of the System Leak Test To locate leaks between the flow sensors and valve S in the EASY nLC 1000 instrument a b To ensure a leak tight connection to port 4 of valve S do the following Disconnect and then reconnect the nanoViper fitting to port 4 of valve S From the Maintenance gt Scripts page start the Analytical Column Equilibration script with the maximum pressure set to 980 bar and the volume set to 140 pL For information about setting up the Analytical Column Equilibration script see step 1b on page 228 When you start the Analytical Column Equilibration script the instrument sets the rotary valves to these positions and begins pumping solvent through flow sensor A e Valve A 1 6 e Valve B Center e Valve S 1 2 e Valve W 1 2 On the Home gt Overview page check the readback for flow sensor A Then depending on the flow rate do one of the following e Ifthe flow rate is lt 100 nL min you have remedied the l
72. ion source g Select the Wash check box to add a Wash step after pump A has pumped the specified volume of solvent A through the system During the Wash step pump S empties 100 pL of solvent A through the loop and needle to the waste bottle 3 Press Start IMPORTANT The Direct Infusion script flushes the solvent lines before it ends Stopping the script before it ends can leave sample solution in the flow path 4 If you press Stop before the script ends follow the instructions in message box that appears to make sure that the current sample is flushed from the system see Figure 43 Figure 43 Wash instructions that appear when you stop the Direct Infusion script Direct infusion Attention Wash is enabled but the script was aborted Make sure you wash loop amp needle before running further infusions e g by specifying zero for the pickup and infusion volumes 54 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific Keeping Service Records Thermo Scientific perform on the instrument To record a service action 1 Press Maintenance gt Log Book 3 Maintenance Scripts and Service Records Keeping Service Records Use the Maintenance gt Log Book page to electronically enter all the service actions that you The Maintenance gt Log Book page opens see Figure 44 Figure 44 Log Book page of the Maintenance menu tab Thermo Home Batch Setup Method Editor Maintenance Configura
73. job 01 05 43 Sample 00 35 54 Gradient 20 00 18 Jul 2011 17 03 admin ta aS SO 7S 10i 125 150 17S 260 225 250 275 300 325 350 375 Exit Time min Sample loading step 4 uL min Precolumn equilibration step 3 5 yL min EASY nLC Series Troubleshooting and Maintenance Guide 179 6 Troubleshooting Troubleshooting Tips System Reaches Its Maximum Pressure During the Gradient If the pressure trace shows maximum pressure during the gradient review the possible causes in Table 14 Table 14 Possible causes of maximum pressure spikes during a run Symptom Possible cause Action The system reaches its Clogged column Run the Back Pressure script for pumps A and B ea If the test passes one of the columns is probably run clogged and needs to be replaced Clogged system Run the Back Pressure script for pumps A and B If the test fails go to Troubleshooting a System Blockage on page 249 Excessively high flow rate Reduce the flow rate in the method When a column or the Column Out line is clogged the back pressure can increase to the maximum system pressure for pump A pump B or pumps A and B during the gradient see Figure 187 Figure 187 Back pressure shown reaching the maximum system pressure for pumps A and B Maximum pressure Maximum pressure reached for pump A reached for pump B Thermo aut Batch Setup Method Editor Maintenance Configuration SCIENTIFIC Overview oy z Pump 6 2 Gra
74. message shown in Figure 196 appears Figure 196 Server error message displayed when pump B cannot generate sufficient pressure to start the gradient Server error message s 20 07 11 11 19 Issue code 87 Pump B unable to reach target pressure within 50 pl Please check for air leaks Reported by Business logic IFC 4 0 ee Close Errors Reported by the Xcalibur Data System Table 17 lists the possible causes of the common errors reported by the Xcalibur data system Table 17 Possible causes of errors reported by the Xcalibur data system Sheet 1 of 2 Symptom An Acquisition Server failure prevents the sequence from running see Figure 197 on page 190 Possible cause Lost connection between the EASY nLC instrument and the external data system computer Action Check the Ethernet connections between the EASY nLC instrument and the data system computer Exit the Xcalibur data system Then open the Thermo EASY nLC Configuration dialog box and click Test Connection If necessary restart the EASY nLC system The Acquisition Server displays the message that the EASY nLC device has reported a failure during the Prepare For Run step Injection volume is greater than the loop size minus 2 pL See Figure 198 on page 191 Check the loop configuration on the Properties page for the EASY nLC HPLC device on the Maintenance gt Devices page Make sure that the configured loop volum
75. min tightening the solvent line connections remedied the leak and you have completed this troubleshooting procedure Return the instrument to normal operation e Ifthe flow is gt 100 nL min the leak is coming from one of the solvent lines Go to the next step Note For information about ordering solvent lines for the EASY nLC II instrument see Table 32 on page 314 4 Replace the solvent line that connects the mixing Tee outlet and port 4 of valve S Restart the Analytical Column Equilibration script and monitor flow sensor A e Ifthe flow is lt 100 nL min you have completed this troubleshooting procedure e Ifthe flow is gt 100 nL min go to the next step 246 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 6 Troubleshooting Troubleshooting the Results of the System Leak Test 5 Replace the solvent line that connects the outlet of flow sensor A to the mixing Tee inlet Restart the Analytical Column Equilibration script and monitor flow sensor A e Ifthe flow is lt 100 nL min you have completed this troubleshooting procedure e Ifthe flow is gt 100 nL min go to the next step 6 Replace the solvent line that connects the outlet of flow sensor B to the mixing Tee inlet Restart the Analytical Column Equilibration script and monitor flow sensor A e Ifthe flow is lt 100 nL min you have completed this troubleshooting procedure e Ifthe flow is gt 100 nL min contact your local Thermo Fishe
76. mixing Tee 3 Remove the nut ferrule and sleeve from the needle tubing Figure 3 Autosampler tubing with a nut ferrule and sleeve at one end Soe 4 Move the z axis needle holder to an accessible location within the tray compartment by doing one of the following e For the ASA model go to step 5 e For the ASC model go to step 6 5 For the ASA model move the z axis needle holder to position Al as follows a Press Home gt Overview b Press the autosampler icon c The Autosampler dialog box opens d In the XYZ Robot area select position Al in the Well box Figure 4 Autosampler direct control dialog box Eject Plate Insert Plate co meade up nonae oom pRPrpmmm Cooler ro su ciate Temperature e Close e Press Goto f Go to step 7 6 For the ASC model move the z axis needle holder to Figure 7 Autosampler needle connected to the zaxis needle holder position W1 as follows ASA zaxis needle holder ASC zaxis needle holder a Press Home gt Overview b Press the autosampler icon The Autosampler dialog box opens c In the XYZ Robot area press Go to Calibration The Tools page of the Autosampler view on the Maintenance gt Devices page opens Figure 5 Tools page of the Autosampler view on the Maintenance gt Devices page Installing a New Autosampler Needle Cooled Autosampler ASC Autosampler Plate Visis Wash To install an new a
77. mouse RS 232 Reserved for hardware add ons or high level MS control by using serial communication P Bus Reserved for instrument add ons using the internal EASY nLC instrument protocol bus 8 wire control and limited power at 9 24 V PS 2 Input for connection of keyboard or mouse discontinued computer box only Monitor Output for connection of external display EASY nLC Series Troubleshooting and Maintenance Guide 25 1 Introduction Specifications Table 8 Hardware components Item Specification Pumps e 140 pL volume enough for a gt 10 h 0 100 B 300 nL min gradient e 1 nL min to 300 pL min flow range e External pressure sensor Rotary valves e VICI Valco rotor stator e 6 ports e 3 positions 1 6 1 2 or CENTERED Autosampler e Peltier cooled Capacity is maximum 20 C 68 F below ambient temperature measured at 60 RH Expect less if you remove the side panels use an adapter plate in the autosampler other than the standard plate or both Plate holder ejects through spring mounted autosampler door e 4 glass bottles with plastic lids for waste or wash liquids 26 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific ee i Maintenance Schedule To familiarize yourself with the maintenance schedule for the EASY nLC instrument review this chapter Contents e Daily Maintenance e Weekly Maintenance e Quarterly Maintenance e Yearly Maintenance e Field Service Repairs
78. nLC Instrument on page 21 64 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 4 Routine Maintenance Maintaining the Syringe Pumps 9 Remove the worn piston seal as follows a To avoid contaminating the pump head clean the piston seal tool and guide tool by soaking them in a beaker filled with 100 HPLC grade methanol Figure 51 shows the piston seal tool Figure 51 Piston seal tool for the PLF model pump Piston seal tool Guide tool b Insert the piston seal tool into the worn piston seal and pull the piston seal out of the pump head Figure 52 Figure 52 Using the piston seal tool to remove the worn piston seal PLF model pump Worn piston seal 10 Install the new piston seal as follows a Insert the guide tube into the pump head flange see Figure 53 Figure 53 Guide tool inserted into the pump head flange PLF model pump Guide tool b Using a pipette fill the groove in the piston seal with methanol Tip Filling the piston seal groove with methanol before installing the seal reduces the time required for the Flush Air script to remove air from the pump Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 65 4 Routine Maintenance Maintaining the Syringe Pumps c Place the new seal with the groove facing upward on the piston seal tool see Figure 54 Figure 54 Piston seal mounted onto the piston seal tool PLF model pump d Inser
79. nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 3 Maintenance Scripts and Service Records Maintenance Scripts c In the Start Mode area select the Contact Closure option Figure 32 Acquire Data dialog box with the Contact Closure option selected Acquire Em Folder EASE AEE AE ET ELE Acquire Time Continuously File Name File st art O Scans 10 Sample Name ioe Pause O Minutes 1 00 i vew Go to Standby when Finished E Use instrument method a SED MSE ITS Acquisition Status Inst Setup State Idle Start Mode Time min 0 000 Immediate Contact Closure D Divert Valve Cancel Help d Click Start The mass spectrometer remains in the Waiting for Contact Closure state until it receives a contact closure signal 5 Send the contact closure signal from the EASY nLC instrument to the MS detector as follows a On the EASY nLC touch screen press Maintenance gt Scripts The Maintenance Scripts page opens b In the Category list select Test c Inthe Name list select MS Connection d Press Start The EASY nLC instrument sends a contact closure signal to the mass spectrometer The contact closure signal triggers the mass spectrometer to start scanning The Scan LED on the front panel of a Thermo Scientific mass spectrometer flashes blue when the contact closure signal is set up correctly Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guid
80. open ended wrench Pipette e PLU pump P N LC501 2 mm hex wrench or ball driver e Pressure sensor P N LC502 2 5 mm hex wrench or e Tubing between the pump and the pressure sensor ball driver Pumps A and S PEEK tubing P N LC212 13 mm open ended wrench Pump B prebent stainless steel tube P N LC215 Small flathead screwdriver Tubing between the pressure sensor and the rotary valve 3 mm open ended wrench Pumps A and S PEEK tubing P N LC213 Pump B prebent stainless steel tube P N LC216 To replace a PLF pump with a PLU pump in the EASY nLC Il instrument Upgrade the touch screen software to version 3 1 or later If the touch screen software is older than version 2 7 8 you must first upgrade to version 2 7 8 and then upgrade to version 3 1 For information about upgrading the software refer to Appendix B of the EASY nLC Series Getting Started Guide for the touch screen software You can find the touch screen software files in the user zone on www proxeon com 154 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 5 Field Service Maintenance Replacing a Pump 2 Before you remove the right side panel do the following a Set the corresponding valve to the Center position as described in Using the Valve Controls on page 204 b Close down the EASY nLC II instrument and then turn off the power switch on the instrument s back panel see Closing Down the EASY nLC I
81. pliers or tweezers insert and tighten the screws that secure the autosampler to the bottom of the housing as follows e Insert and tighten the screw on the back left side of the autosampler see Figure 120 on page 126 e Insert and tighten the screw on the right side of the autosampler see Figure 119 on page 125 e Insert and tighten the screw on the front of the autosampler see Figure 121 on page 126 Insert and tighten the screws that secure the back horizontal cable bar to the autosampler frame see Figure 117 on page 124 Install a new tie wrap to secure the cables to the vertical part of the autosampler frame Plug the bus cables into the autosampler cooler and horizontal XYZ axis PCBs Reinstall the computer box as described in To install the new computer box on page 112 Reinsert the autosampler needle into the holder and tighten the fitting Mount the right side panel to the instrument housing Then with a 2 screwdriver secure the panel by rotating the three captive screws a quarter turn clockwise Tip Reconnect the left side panel to the housing after you recalibrate the autosampler Reconnect the power cable Turn on the EASY nLC instrument Add the ASC autosampler to the Devices list on the Maintenance gt Devices page as follows a Press Maintenance gt Devices to open the Maintenance gt Devices page b Press Add Devices to open the Select the Device to Add dialog
82. plug see Figure 105 on page 114 Using a2 mm ball driver and the screws that you removed in step 5 on page 114 secure the monitor to the instrument Depending on the instrument model do the following e For the EASY nLC II instrument use a Torx T 10 wrench and the 10 screws that you removed in step b on page 113 to secure the front plate to the housing e For the EASY nLC 1000 instrument use a Torx T 10 wrench and the three screws that you removed in step 4 on page 114 to secure the top front panel to the housing Mount the left side panel to the instrument housing Then with a 2 screwdriver secure the panel by rotating the three captive screws a quarter turn clockwise Plug in the power cable and then turn on the EASY nLC instrument 116 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 5 Field Service Maintenance Replacing a Pressure Sensor for a PLF Pump Replacing a Pressure Sensor for a PLF Pump The procedure for replacing a pressure sensor differs between EASY nLC 1000 instruments with PLU pumps and EASY nLC II instruments with PLF pumps Note Most EASY nLC II instruments contain the PLF pump model The following procedure explains how to replace a pressure sensor that is connected to a PLF pump For instructions about replacing a pressure sensor that is connected to a PLU pump see Replacing a Pressure Sensor for the PLU Pump on page 90 CAUTION Wear powder free glove
83. pump e Aborting unable to build pressure e Aborting check solvent level Note For information about the Flush Air script see Prepare Flush Air on page 33 The following procedure systematically checks for a blockage in the check valve assembly in the solvent line that connects the pressure sensor to the valve and in the pump head CAUTION Wear powder free gloves and safety glasses when handling parts of the LC system that come into contact with solvents To determine why the pump has failed the Flush Air script 1 If you have not already done so remove the right side panel of the instrument 2 Depending on the pump do one of the following e For pumps A and B go to step 3 e For pump S go to step 4 on page 213 3 For pumps A and B determine whether the check valve assembly is functioning as follows a Run the Purge script for the pump that has failed see Prepare Purge Solvent on page 32 b Check that solvent is being aspirated and dispensed correctly through the Teflon lines e Ifthe pump is drawing solvent during the fill portion of the purge cycle the solvent side check valve is working properly Ifthe pump is dispensing solvent into the waste beaker during the empty portion of the purge cycle the waste side check valve is working properly Note Ifa check valve is not working properly replace it as described in Replacing the Check Valves on page 80 c Ifth
84. remove the panel 7 Remove the USB LVDS and monitor cables connected to the computer box being careful not to damage the LVDS plug see Figure 105 Figure 105 USB LVDS and monitor cables USB cable LVDS cable Monitor cable 114 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 5 Field Service Maintenance Replacing the Monitor 8 If a white plastic needle guide is installed on the back of the monitor remove it before removing the monitor Figure 106 shows the white plastic needle guide on the back of the monitor Figure 106 White plastic needle guide Needle guide 9 Carefully remove the monitor by pulling it straight forward see Figure 107 Figure 107 Removing the monitor Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 115 5 Field Service Maintenance Replacing the Monitor To install the new monitor Carefully insert the new monitor into the instrument housing If you removed the white plastic needle guide on the back of the monitor reinstall it Figure 106 on page 115 shows the white plastic needle guide mounted to the back of the monitor If the computer box has a PS 2 keyboard connector on its back panel as shown in Figure 16 on page 16 connect the adapter cable that is supplied with the new monitor to the monitor cable Connect the USB LVDS and monitor cables to the computer box being careful not to damage the LVDS
85. same for the EASY nLC 1000 instrument and the EASY nLC II instrument CAUTION Wear powder free gloves and safety glasses when handling parts of the LC system that come into contact with solvents Replacing a high pressure valve requires these items Tools Parts and materials e 2 Phillips screwdriver e Powder free gloves e 1 4 in open ended wrench e Switching valve e Torx T 10 wrench EASY nLC 1000 instrument P N LC519 e 2 5 mm L hex wrench oe Flathead screwdriver EASY nLC II instrument P N LC218 To replace a rotary valve follow these steps 1 To remove a rotary valve on the page 2 To install a new rotary valve on page 149 To remove a rotary valve 1 Close down the EASY nLC instrument see Closing Down the EASY nLC Instrument on page 21 and then unplug the power cable 2 Remove the computer box as described in To remove the computer box on page 108 Note Removing the computer box requires the removal of the left side panel and the back panel 3 Using a 2 Phillips head screwdriver with a quarter turn loosen the three captive screws that secure the right side panel to the instrument housing Then remove the panel 4 From the right side of the instrument solvent system compartment remove the tubing from the stator on the valve that you are replacing Be careful to avoid pulling any fused silica lines as there is limited space around the valves Tip When replumbing th
86. script for Pump S with a flush volume threshold of 10 pL for the EASY nLC II system or 12 uL for the EASY nLC 1000 system For information about running the Flush Air script see Prepare Flush Air on page 33 88 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 4 Routine Maintenance Replacing the Sample Loop Replacing the Sample Loop Thermo Fisher Scientific provides sample loops in several sizes for the EASY nLC instruments To replace the sample loop 1 Using a 2 Phillips head screwdriver with a quarter turn loosen the three captive screws that secure the right side panel to the instrument housing Then remove the panel 2 To remove the sample loop from the valve do one of the following e For the EASY nLC II instrument use a 1 4 in open ended wrench to loosen the fittings that secure the sample loop to ports 2 and 5 of valve S Then remove the sample loop from the valve Figure 80 shows the sample loop connections for the EASY nLC II instrument Figure 80 Sample loop connections for the EASY nLC II instrument Sample loop e For the EASY nLC 1000 instrument do the following a Return the system to atmospheric pressure see Returning the System to Atmospheric Pressure on page 325 b Disconnect the nanoViper fittings connected to ports 2 and 5 of valve S Figure 81 shows the sample loop connections for the EASY nLC 1000 instrument Figure 81 Sample loop connections
87. the USB memory stick EEx Fie Edit View Favorites Tools Help ae Q sek Q w P Search Folders Eii Address E Go Name Size Type Date Modified File and Folder Tasks ybatchlog zip 31KB Compressed 24 10 2005 12 23 3 El etc zip 3KB Compressed 24 10 2005 12 23 E export zip 1 K6 Compressed 24 10 2005 12 23 Publish this Folder to loafiles zip 35KB Compressed 24 10 2005 12 23 g Make a new folder the Web g Share thi folder maintlog zip 31KB Compressed 24 10 2005 12 23 98 0KB Y My Computer 294 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific Thermo Scientific 8 Remote Support Saving System Files on a USB Removable Storage Device Table 28 Zipped files created by pressing Copy Log Files File name Description batchlog zip Contains one folder for each batch you have run with information on samples methods and EASY nLC device performance etc zip Contains system configuration files export zip Contains exported batches and methods per user and system backup logfiles zip Contains system log files with information on overall system performance maintlog zip Contains one folder for each maintenance script you have run with information on EASY nLC device performance If you have problems with your system forward the files to Thermo Fisher Scientific Technical Support for assistance EASY nLC Series Troubleshooting and Maintenance Guide 295 8
88. the mass spectrometer chassis or the data system computer as follows a Connect one probe to an unpainted surface on the EASY nLC chassis and connect the other probe to an unpainted surface on the external hardware chassis see Figure 236 Figure 236 Resistance measurement Multimeter set up to take a resistance measurement b Ensure that the resistance measurement is from 0 to 10 ohms 268 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific ee Calibrating the Autosampler s XYZ Robot To calibrate the autosampler s XYZ robot follow the instructions in this chapter Contents e Replacing the Autosampler Adapter Plate e Managing Plate Formats e Preparing the Autosampler for the Calibration Routines e Calibrating Plates e Calibrating the Wash Bottle and Extra Vial Positions For the ASC autosampler the calibration covers the following areas of the autosampler Microtiter plate or vial plate l Extra vials Waste bottle with needle wash insert Wash solvent bottles Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 269 7 Calibrating the Autosampler s XYZ Robot Replacing the Autosampler Adapter Plate For the legacy ASA autosampler the calibration covers the following areas of the autosampler Microtiter target Wash waste plate bottles Calibrate the autosampler in the following situations e When you want to use a new type of vial or microwell pl
89. the pump 5 To locate a leak between the pressure sensor and the valve do the following a Remove the blanking nut and replace the original fitting b Manually check the fittings between the pressure sensor and the valve and confirm that they are leak tight If the fittings are leak tight the valve might be the source of the leak c Ifthe valve is leaking replace the worn rotor seal and clean or replace the stator in the affected valve see Maintaining the Rotary Valves on page 74 d Run the Test Leaks script for the relevant pump and confirm that you have fixed the leak 6 To locate a leak between the pressure sensor and the pump do the following a Remove the blanking nut and replace the original fitting b Confirm that the fittings between pump and the pressure sensor are leak tight c Repeat the Test Leaks script d Ifthe script fails again the cause might be a worn piston seal e Ifyou suspect that the leak is caused by a worn piston seal go to Maintaining the Syringe Pumps on page 60 Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 217 6 Troubleshooting Running a System Leak Test Running a System Leak Test To identify and locate a leak downstream from valves A and B run the system leak test Test Leaks script with System selected and then take appropriate action as described in Table 24 on page 221 Table 23 lists the materials required to identify a
90. the three pressure sensors connect the solvent path between the pump outlet and the associated rotary valve Using the strain gauge principle to measure pressure accurately the pressure sensors are flow through zero dead volume devices with a wetted path that contains 12 pL of swept volume The pressure sensors for the EASY nLC II instrument have an operating range of 0 to 5000 psi 345 bar and the pressure sensors for the EASY nLC 1000 instrument have an operating range of 0 to 15 000 psi 1034 bar The two flow sensors provide feedback control for pumps A and B The flow sensors are liquid mass flow meters with a CMOSens patented technology Each flow meter consists of a length of fused silica capillary a sensor chip a heating resistor and two temperature sensors see Figure 9 Figure 9 Flow sensor schematic Heater Temperature sensors Sensor chip Based on CMOSens Technology Principle patented US Pat 6 813 944 10 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 1 Introduction Hardware Components The flow sensors determine the flow rate of solvents passing through the system by measuring the heat transfer through the fused silica capillary Outside the capillary a heating resistor on a thermally optimized membrane is maintained above ambient temperature When liquid flows through the capillary the temperature distribution upstream and downstream of the heating resistor is disturbe
91. these functions Component Function Pumps A and B Draw solvent from the solvent bottles on top of the instrument empty solvent to the waste beaker and deliver solvent to the mixing Tee Pump S Draws solvent from the W3 bottle draws sample from the specified sample location into the autosampler needle and empties solvent to the W4 bottle in the autosampler tray compartment For maintenance purposes the system tracks the volume pumped by each pump see Checking and Resetting the Device Usage Counters on page 56 The PLF model pumps installed in the EASY nLC instrument have an error LED The LED illuminates green when the instrument is turned on and the pump is functioning properly Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 9 1 Introduction Hardware Components Pressure Sensors Flow Sensors The LED panels for the PLU model pumps installed in the EASY nLC 1000 instrument provide the following status information LED States Flow sensor for Illuminates blue the instrument is turned on and the flow sensor is Pumps A and B connected to the pump Not illuminated the instrument is not turned on or the flow sensor is not connected to the pump Power Iluminates yellow The instrument power is turned on Not illuminated The instrument power is turned off Error Iluminates red A pump error has occurred Not illuminated The pump is operating properly For pumps A B and S
92. this Thermo Fisher Scientific instrument requires a team effort to lift and or move the instrument This instrument is too heavy and or bulky for one person alone to handle safely Notice on the Proper Use of Thermo Scientific Instruments In compliance with international regulations This instrument must be used in the manner specified by Thermo Fisher Scientific to ensure protections provided by the instrument are not impaired Deviations from specified instructions on the proper use of the instrument include changes to the system and part replacement Accordingly order replacement parts from Thermo Fisher Scientific or one of its authorized representatives Notice on the Susceptibility to Electromagnetic Transmissions Your instrument is designed to work in a controlled electromagnetic environment Do not use radio frequency transmitters such as mobile phones in close proximity to the instrument For manufacturing location see the label on the instrument Declaration of Conformity for the EASY nLC II System U S Safety and EMC Electromagnetic Compliance Standards Safety This instrument has been reviewed for compliance with standard ANSI UL 3101 1 Electrical Equipment for Laboratory Use Part 1 General Requirements 1st Edition EMC This device complies with Part 15 of the FCC Rules Operation is subject to the following two conditions 1 This device may not cause harmful interference and 2 This device m
93. this procedure To test the network connection between the data system computer and the EASY nLC instrument 1 From the Windows Start menu choose Run The Run dialog box opens 2 In the Open box type cmd 3 Press OK The Command Prompt window opens 4 Type telnet ZP address for the EASY nLC system 6666 see Figure 234 266 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 6 Troubleshooting Troubleshooting Communication Problems Figure 234 Command Prompt window with the default IP address for the EASY nLC instrument ct C WINDOWS system32 cmd exe Microsoft Windows KP Version 5 1 2666 lt C gt Copyright 1985 2661 Microsoft Corp C Documents and Settings lab gt telnet 172 16 00 103 6666 5 Press the ENTER key e Ifthe connection is set up correctly a login prompt appears in the Telnet window see Figure 235 e Ifthe connection is not set up correctly the following message appears Could not open connection to host Figure 235 Telnet window with login prompt ci Telnet 172 16 0 103 lof x Connected from 172 16 6 1611 172 16 6 161 41721 a Guessed Locale en E Negotiated Terminal Info ANSI 80 25 Kernel Core messageCenter Login to use system login Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 267 6 Troubleshooting Verifying that the LC MS System Is Properly Grounded Verifying that the LC MS System Is Properly Grounded
94. to the next procedure Priming the Pump 70 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 4 Routine Maintenance Maintaining the Syringe Pumps Priming the Pump After you replace a piston seal prime the pump with the appropriate solvent before you reconnect the solvent line to the pump head To prime the pump 1 Turn on the EASY nLC instrument and log in as an administrator 2 Set the piston to its fully extended position as follows a On the touch screen press Home gt Overview b Press the pump icon for the pump that you want to control The Pump X dialog box opens where X identifies the pump A B or S c Set the flow rate to 300 pL min and the volume to 140 pL d Press Start 3 Fill the pump head with solvent as follows a Pipette an aliquot of the appropriate solvent into the pump head see Figure 65 Figure 65 Adding solvent to the pump head CAUTION Be careful not to spill solvent on the PCBs e For the PLU pump model standard in the EASY nLC 1000 instrument and replacement part for both EASY nLC instruments the LED panel cover for each pump protects the PCB from accidental contact when the instrument s right panel is removed However the cover does not form a waterproof seal with the pump body so that the PCBs are exposed to solvent leaks from above e For the PLF pump model EASY nLC II instrument the printed circuit boards are completely exposed Thermo Scientific E
95. tubing that connects the pump to its pressure sensor 7 If the problem is in the pump verify that the piston is not dirty broken or scratched and replace the piston seal as described in Maintaining the Syringe Pumps on page 60 Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 215 6 Troubleshooting Troubleshooting a Pump that Fails the Leaks Script Troubleshooting a Pump that Fails the Leaks Script Follow this procedure to determine why the pump failed the Leaks script Note For information about running the Leaks script see Test Leaks on page 44 In the Value column on the Parameters page of the Leaks script select A to test pump A or B to test pump B This procedure systematically checks whether the following problems caused the Leaks script failure e Excess air in the system e Tubing connections between the pump and the pressure sensor e Tubing connections between pressure sensor and the valve e A worn piston seal in the pump head e A worn rotor seal in the subsystem valve CAUTION Wear powder free gloves and safety glasses when handling parts of the LC system that come into contact with solvents To troubleshoot a pump that has failed the Leaks script 1 Run the Flush Air script for the failed pump see Prepare Flush Air on page 33 Enter the following value for the Flush Volume parameter e For the PLF model pump enter 10 pL e For the PLU model pump
96. using the Flow Sensors script see Calibrate Flow Sensors on page 51 EASY nLC Series Troubleshooting and Maintenance Guide 99 4 Routine Maintenance Replacing the Hard Drive Replacing the Hard Drive Use this procedure to replace the hard drive in either the EASY nLC 1000 instrument or the EASY nLC II instrument Thermo Fisher Scientific supplies several versions of the hard drive Order the appropriate hard drive based on both the instrument model and the computer box where you are replacing the hard drive The discontinued versions of the computer box have a PS 2 port see Figure 91 Figure 91 Comparison of computer ports between the obsolete and current computer boxes Obsolete computer box Current computer box exe ea ES OMe Si D PS 2 port on the back panel of the obsolete computer box Replacing the hard drive requires these tools and materials Tools Replacement part 2 5 mm hex wrench Hard drive Torx T 10 wrench e EASY nLC II instrument Compatible with obsolete computer box P N LC281 Compatible with current computer box P N LC286 or e EASY nLC 1000 instrument Compatible with obsolete computer box P N LC581 Compatible with current computer box P N LC586 To replace the hard drive follow these steps 1 To remove the hard drive on page 101 2 To install the hard drive on page 103 100 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientifi
97. well position box Goto Well a m Needle Up J Needle Down J Wash X Wi J w2 J w3 J Ww J Cooler Sim Temperature input box a 7 0 7 Set Disable Temperature Temperature readback box Close 6 7 C 210 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific Thermo Scientific 6 Troubleshooting Using the Direct Controls for Troubleshooting and Maintenance To start the calibration procedure for the current plate format Press Goto Calibration The Tools view for the autosampler on the Maintenance gt Devices page opens To calibrate the XYZ robot for the plate format follow the instructions in Calibrating Plates on page 280 To move the needle to a vial or well position In the Vial or Well box enter a vial or well position Press Goto To move the needle to a wash bottle position Press W1 W2 W3 or W4 To change the cooler temperature Enter a temperature in the temperature input box Press Set Monitor the temperature change in the temperature readback box EASY nLC Series Troubleshooting and Maintenance Guide 211 6 Troubleshooting Troubleshooting a Pump that Fails the Flush Air Script Troubleshooting a Pump that Fails the Flush Air Script If pump A B or S fails the Flush Air script and the Output page displays one of the following messages a leak or blockage could be preventing solvent from entering the
98. you felt the initial resistance IMPORTANT To prevent damage to the sealing surface of the nanoViper fitting take care not to overtighten the fingertight nanoViper fitting 2012 Thermo Fisher Scientific Inc Thermo All rights reserved SCIENTIFIC nanoViper Figure 2 shows the nanoViper tubing connections in the EASY nLC 1000 instrument Tubi ng e Port 6 of valve A to flow sensor A inlet Connections e Port 6 of valve B to flow sensor B inlet e Flow sensor A outlet to mixing Tee inlet e Flow sensor B outlet to mixing Tee inlet e Mixing Tee outlet to port 4 of valve S e Sample loop connected to ports 2 and 5 of valve S e Column Out line connected to port 3 of valve S and the HPLC union e Waste In line connected to port 2 of valve W and the HPLC union or the venting Tee Figure 2 nanoViper tubing connections in the EASY nLC 1000 instrument Valve A Column Out line Valve S Vie VICI EKS Flow sensor A Valve B 2 Sample loop rf Waste In line Flow sensor B 324 Returning the System to Atmospheric Pressure Upstream of Valve S Solvent Lines Before you disconnect a nanoViper fitting from its receiving port always return the solvent line to atmospheric pressure The high pressure solvent lines in the EASY nLC solvent system can be divided into two categories based on their position relative to valve S e Upstream of Valve S Solvent Lines e Downstream of Valve S Sol
99. 1 3 Maintenance Scripts and Service Records Maintenance Scripts Prepare Purge Solvent The Purge Solvent script fills the selected pump or pumps with solvent and then ejects it into the waste beaker Use the Purge Solvent script when exchanging solvents removing air from the solvent lines or filling the pumps Figure 24 shows the parameters for the Purge Solvent script Figure 24 Purge Solvent script parameters Thermo Home Ete T a o a N Eo aa Maintenance Meera dtelele iielg SCIENTIFIC Category Name f p Fis _ No graph a Log Book Prepare X urge solvent Support pe Parameters SEOs Devices Parameter v Value Purge iterations 2 i i i amp amp amp o o o slouls c c c aala mead e hsze f Job 00 00 21 _ No graph X 11 May 2011 11 36 admin Schedule Exit Torun the Purge Solvent script 1 Open the Purge Solvent script as follows a On the touch screen press Maintenance gt Scripts b In the Category list select Prepare c Inthe Name list select Purge Solvent 2 Set up the parameters for this script as follows a Press the Parameters tab b In the Purge Iterations box enter the number of empty fill cycles to be performed To refresh solvents on a daily basis enter 2 iterations e To exchange solvents enter a minimum of 10 iterations Note Entering a value of zero 0 for purge iterations refills the pump c
100. 1 Depending on the instrument model do one of the following e For the EASY nLC II instrument do the following Using a 1 4 in open ended wrench tighten the stainless steel fitting connected to port 6 of valve B Hand tighten the PEEK fitting connected to the flow sensor inlet e For the EASY nLC 1000 instrument return the solvent system upstream of valve S to atmospheric pressure as described in the Using nanoViper Fittings Quick Reference Guide on page 323 Then individually disconnect and reconnect the nanoViper fittings connected to port 6 on valve B and the flow sensor B inlet Take care not to overtighten these fingertight fittings EASY nLC Series Troubleshooting and Maintenance Guide 223 6 Troubleshooting Troubleshooting the Results of the System Leak Test 2 Rerun the system leak test Ifthe system leak test still fails go to To troubleshoot a leak in valve B e Ifthe system leak test passes return the system to normal operation To troubleshoot a leak in valve B 1 Clean the rotor and stator in valve B as described in Cleaning the Rotor Seal and Stator on page 74 2 If necessary replace the rotor seal as described in Replacing the Rotor Seal on page 77 Locating a Leak in the Solvent Path from Pump A to Flow Sensor A The system leak test returned the following message see Table 24 on page 221 Check from pump A to flow sensor A for leaks Pump A flow loss gt 1000 nL m
101. 1 shows the reduced time for the precolumn equilibration and sample loading steps when the precolumn is damaged and has lost a significant portion of its packing material The flow rate for the column equilibration and sample loading steps is higher gt 15 L min and the duration is shorter because the precolumn is exerting less back pressure Note The specifications for the precolumns used to acquire the data in Figure 185 and Figure 186 are as follows e 2cm length 100 pm ID e 5 um particle size e C18 Figure 191 Typical pump A profile for a damaged precolumn The measured flow rate is gt 15 pL min Spratt Method Editor Maintenance Configuration Overview Pump A frapns Desired flow Real flow Pressure 110 000 0 Queue 100 000 0 90 000 0 80 000 0 70 000 0 60 000 0 50 000 0 40 000 0 30 000 0 C 20 900 07 10 000 0 Flow ni mi s ea anssasd job 01 16 47 40 000 0 Sample 00 28 26 50 000 0 Gradiem 20 00 60 000 0 20 jul 2011 80 000 0 13 21 admin 0 25 5 0 75 10 0 125 15 0 175 200 225 25 0 27 5 Time min Exit Sample loading step Column equilibration step Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 185 6 Troubleshooting Troubleshooting Tips Weak or Absent Signal Leak Upstream of Flow Sensor B Figure 192 shows a typical pump B profile for a system with a leak upstream of flow sensor B Flow sensor B displays a negativ
102. 2 Check the sample loop for leaks Go to Checking the Sample Loop Connections for a Leak on page 222 Check the B solvent line valve to flow sensor and valve B for leaks Flow sensor B 30 nL min Check from pump A to flow sensor A for leaks Pump A flow loss gt 1000 nL min Go to Locating a Leak in the Solvent Path from Valve B to Flow Sensor B on page 223 Go to Locating a Leak in the Solvent Path from Pump A to Flow Sensor A on page 224 Check from valve S to valve W including valve W for leaks Go to Locating a Leak In or Between Valve S and Valve W on page 226 Check solvent lines between flow sensor A and valve S including the mixing Tee and flow sensor B for leaks Go to Locating a Leak Between the Flow Sensors and Valve S on page 234 EASY nLC Series Troubleshooting and Maintenance Guide 221 6 Troubleshooting Troubleshooting the Results of the System Leak Test Checking Valve S for a Leak The system leak test returned the following message see Table 24 on page 221 Check valve S for leaks Flow sensor A gt 100 nL min The most likely cause of this test result is a leak in the flow path between ports 3 and 4 of valve S To fix the leaking valve 1 Clean the rotor seal and stator in valve S with methanol and a lint free swab see Cleaning the Rotor Seal and Stator on page 74 2 Repeat the system leak test script and if it fails again r
103. 27 244 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 6 Troubleshooting Troubleshooting the Results of the System Leak Test Figure 227 Solvent line disconnected from the outlet of flow sensor B Inlets of the EASY nLC 1000 flow sensors BE x Inline filter connected to the outlet of flow sensor B Solvent line disconnected from the outlet of flow sensor B and plugged with a union and a blind fitting c Reset valve A to position 1 6 d Check the readback for flow sensor A Then depending on the flow rate do one of the following e Ifthe flow rate is lt 100 nL min do the following iii Stop the Analytical Column Equilibration script Remove the damaged inline filter from the outlet of flow sensor B and install a new inline filter For ordering information see Table 35 on page 319 Reconnect the system plumbing and rerun the system leak test see Running a System Leak Test on page 218 Ifthe flow rate is gt 100 nL min do the following iii Thermo Scientific Stop the Analytical Column Equilibration script Install a new solvent line between the flow sensor B outlet and the mixing Tee inlet For ordering information see Table 33 on page 316 Rerun the system leak test see Running a System Leak Test on page 218 EASY nLC Series Troubleshooting and Maintenance Guide 245 6 Troubleshootin
104. 277 Selecting a Plate Format To select the plate format that you want to use 1 Open the Tools view for the autosampler as follows a Press Maintenance gt Devices b In the Devices list select the autosampler device c Press the Tools tab Figure 242 shows the Tools view for the autosampler 274 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 7 Calibrating the Autosampler s XYZ Robot Managing Plate Formats Figure 242 Tools view for the autosampler Thermo Home Ba od Editor SCIENTIFIC Configuration Scripts Devices Log Book Name Type Add Device Support _ Cooled Autosampler ASC Autosampler Autosampler Devices Il EASY nLC HPLC HPLC Piston Pump flow feedback Pump A Piston Pump wi flow fee Remove Device stk Plate Vials Wash f f i Vi Calibrate aan eee New Calibrate Current Vials f Manipulator 6x8 Vials a ee 23 aug 2010 Reset 12 38 Copy To admin Exit Copy To Calibrate Selected plate format button button 2 Select a plate format from the Plate list 3 Press Use Tip If you are using a Thermo Scientific software application to control your liquid chromatograph mass spectrometer LC MS system the plate selection for the EASY nLC system in your Thermo Scientific instrument control software must match the touch screen application selection Creating a New Plate Format To
105. ASY nLC Series Troubleshooting and Maintenance Guide 71 4 Routine Maintenance Maintaining the Syringe Pumps b In the Pump dialog box set the flow rate to 300 L min and the volume to 140 pL Then press Start The piston moves down drawing the solvent into the pump c Make sure the pump head is filled with solvent during the entire retraction of the piston 4 Reconnect the solvent line to the pump head as follows e Fora PLF pump with a PEEK fitting see Figure 48 on page 63 use a 13 mm open ended wrench to tighten the fitting e Fora PLF pump with a stainless steel fitting see Figure 49 on page 63 or a PLU pump use a 1 4 in open ended wrench to tighten the fitting 5 Mount the right side panel to the instrument housing Then with a 2 Phillips head screwdriver secure the panel by rotating the three captive screws a quarter turn clockwise After you reconnect the pump head to the pump body and the high pressure line from the pressure sensor to the pump head reset the pump usage counter and then remove air from the system Note You reset the usage counters before removing air from the system because the air removal procedure requires the pump to go through 10 purge cycles Resetting the Pump Usage Counter Reset the pump usage counter after you prime the pump To reset the pump usage counter 1 On the touch screen press Maintenance gt Devices 2 Select the pump from the list of devices 3 Press the S
106. ASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific Checking Sample Pickup 6 Troubleshooting Checking Sample Pickup When you run the Sample Pickup script the autosampler withdraws a specified volume from a specified position To verify the volume withdrawn by the autosampler you must place a known volume into the specified position and then measure the remaining volume after you run the script You can use a pipette or an analytical balance with microgram accuracy to determine the initial and final volumes 1 Fill the well or vial with a 12 pL of solvent A 2 Weigh the microwell plate or vial 3 Insert the tray into the tray compartment 4 Press Maintenance gt Scripts To check sample pickup from a microtiter plate or vial 5 Run the Sample Pickup script with a pickup volume of 10 pL and your default flow see Figure 232 Figure 232 Sample Pickup script under the Maintenance menu tab Category Name Category Name Category Name f Test f Sample pickup f Test f Sample pickup f f Parameters Output Description Description Parameters Output Test autosampler sample pick up Please fill plate well with a defined volume before running test Max volume Loop volume 2pl Max flow 40 0 pl min Parameter v Value Volume ul 10 00 Flow pl min 20 00 Position 0 A1 Sample pickup started Prime pump filling
107. Accept Go to Calibrating Plates on page 280 to calibrate the vial or well positions 276 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 7 Calibrating the Autosampler s XYZ Robot Managing Plate Formats Deleting a Plate Format To delete a plate format 1 Open the Tools view for the autosampler on the Devices page of the Maintenance menu see step 1 on page 275 2 Select a plate format from the Plate list Note You cannot delete the plate in use When you select the plate in use highlighted in bold text the Delete button becomes unavailable 3 Press Delete The Delete Plate Data dialog box opens 4 Press Accept Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 277 7 Calibrating the Autosampler s XYZ Robot Preparing the Autosampler for the Calibration Routines Preparing the Autosampler for the Calibration Routines To prepare the autosampler for the calibration routines you must remove the left side panel from the instrument and load the appropriate adapter plate vials or microtiter plate wash bottle W4 with insert and optional extra vial into the tray compartment CAUTION Calibrating the autosampler requires removal of the side plate and visual inspection of the autosampler needle holder Because the autosampler compartment contains moving parts and sharp needles make sure to keep hands clear when operating the autosampler during calibration C
108. B storage device formatted with the file system FAT FAT16 and at least 128 MB of free space To copy system files to a USB storage device 1 Press Maintenance gt Support The Support page of the Maintenance menu opens see Figure 252 on page 290 2 Insert the USB storage device into the connector panel on the back of the EASY nLC instrument Choose between one of two USB ports on the upper left side of the panel see Figure 254 Figure 254 USB port location on the back panel of the EASY nLC instrument a 3 In the Log File Copy area select the To USB Removable Storage option Note When you select the To Home Directory option the EASY nLC system creates zip files in the admin folder For information about accessing the admin folder from another computer on your local network refer to the EASY nLC Series Getting Started Guide Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 293 8 Remote Support Saving System Files on a USB Removable Storage Device 4 Press Copy Log Files When the copying is finished the following confirmation appears Copied successfully It is now safe to remove the USB storage Q Close 5 Press Close and remove the USB storage device from the connector panel 6 Insert the USB storage device into your computer Five zipped files appear on the USB storage device see Figure 255 and Table 28 on page 295 Figure 255 Windows XP view of the files on
109. CURRENT_VALUE A 83 ERROR_VALVE_ DEVICE Spurious angle see issue description For valve serial number SPURIOUS_ANGLE in position POSITION lt V 009800 run the Valve ANGLE Tune script 84 ERROR APPLICATION HISTORY TRACE Application state Cycle EASY nLC power _STATE empty or illegal 85 IC_STATUS_FLAG _ Monitor reported Act according to the error WARNING flag s raised log file message only 86 IC_STATUS_FLAG Monitor reported Act according to the error _SEVERE flag s raised in user message interface current run stopped 87 IC_LC_CONDITION_ Waiting for some Failed reaching a Troubleshoot the LC UNSATISFIED condition during run specific flow pressure system for leaks and air unsuccessful etc within a specified volume period of time See Troubleshooting the Results of the System Leak Test on page 221 88 IC_STATUS_FLAG _ Monitor reported Act according to the error ERROR flag s raised in user interface message Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 309 A Error Codes Table 30 Error codes Sheet 8 of 8 No Issue code name Description Cause Solution 89 IC_VALVE_MULTIPLE_ Needed 2 attempts to A switching valve Clean the rotor seal and the ATTEMPTS switch to position 1 6 needed multiple stator Replace the rotor attempts to reach the seal if necessary target position If the problem persists do the following e For valve serial number lt
110. Checking Sample Pickup ee ap teed pac hetan i aeiad whites wate erat 263 Troubleshooting Communication Problems 00 00000 008 264 Network Access is 4 fs Wich s as heath ier se phates ck eee usta es 264 Network Connection Failures 0 0 0 eee cee eee 265 Testing the Network Connection to the EASY nLC Computer 266 Verifying that the LC MS System Is Properly Grounded 268 Chapter7 Calibrating the Autosampler s XYZ Robot 0ceeeeeeeeeee 269 Replacing the Autosampler Adapter Plate 2 0 0 ee eee eee eee 270 Replacing the Adapter Plate in the ASC Model Autosampler 271 Replacing the Adapter Plate in the ASA Model Autosampler 272 Managing Plate Poriiats 5 iets anand dia vs titania othe aree a ai ai Sainte ali 274 Selecting a Plate Format osse hoes tak ew eda aw ee Baie ERA Eee 274 Creating a New Plate Format eaccans tacewdes peewee tan tes 275 Deleune a Plate Fortnat lt d 0 0 st ae pees ee ead Ka eek Rha eed whee 277 Preparing the Autosampler for the Calibration Routines 278 Calibrating Plates ect Sse ths ora e eeu Nee i hehehe hie bile 280 Calibrating the Wash Bottle and Extra Vial Positions 0 0 285 Chapter8 Remote Support ccc eee e eee eee eee eee eee eens 287 Connecting the EASY nLC Instrument to the Support Server 288 Saving System Files on a USB Removable Storage Device 293 Downloading t
111. Completed dialog box appears 296 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific Thermo Scientific 8 Remote Support Downloading the Latest Firmware File Figure 257 shows the transfer information for an instrument with firmware version 2 4 Figure 257 Information for upgrading from a previous version x Transfer of log and firmware files completed Transfers completed The log files have been transferred to the remote support server and the firmware file has been downloaded to the instruments firmware folder To upgrade the application software you should now shut down and power cycle the instrument When the instrument restarts double tap the Upgrade button There are 4 further upgrades to download and install after this one After the downloaded upgrade has been installed you should connect to the Remote Support Server again to fetch the next upgrade 3 Click OK 4 To begin the firmware upgrade do the following a C d Press Exit in the lower left corner of the touch screen A confirmation dialog box opens see Figure 22 on page 21 Press Power Down The EASY nLC application displays a white screen with a small progress bar When the progress bar is completely filled a message appears indicating you can safely turn off the instrument Turn off the instrument and then turn it back on When the Upgrade button appears double tap it 5 If the complete upgrade re
112. Guide 233 6 Troubleshooting Troubleshooting the Results of the System Leak Test b With the Analytical Column Equilibration script running monitor flow sensor A Then depending on the flow rate do one of the following e Ifthe flow is lt 100 nL min install a new Column Out line Then reconnect the solvent lines and rerun the system leak test as described in Running a System Leak Test on page 218 For ordering information see Appendix C Consumables and Replacement Parts e Ifthe flow is gt 100 nL min remedy the leak in valve S as described in Maintaining the Rotary Valves on page 74 Then rerun the system leak test as described in Running a System Leak Test on page 218 Locating a Leak Between the Flow Sensors and Valve S When one or more of the connections are leaking in the solvent path between the flow sensor outlets and the mixing Tee inlets the system leak test returns this message see Table 24 on page 221 Check solvent lines between flow sensor A and valve S including the mixing Tee and flow sensor B for leaks As the flow sensors and mixing Tee differ for the EASY nLC 1000 and EASY nLC II instruments follow the topic for your instrument to locate and remedy the leak e EASY nLC 1000 Locating a Leak Between the Flow Sensors and Valve S on pages 235 245 e EASY nLC II Locating a Leak Between the Flow Sensors and Valve S on page 246 234 EASY nLC Series Troubleshooting and
113. HT MTP gt Needle In Out 6 rNeedle Up Down 6 DE Needle Lef Right 4 A m 3 Press the green vial or well in the upper left corner of the plate view 1 Joa The needle moves to the current saved coordinate The saved coordinates for the x and y axis appear on the left side of the slash the needle does not descend into the vial or well and the z axis value on the left side of the slash remains at 0 The values on the left side of the slash change as you adjust the needle position Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 281 7 Calibrating the Autosampler s XYZ Robot Calibrating Plates 4 Center the needle above the vial or well as follows Press the Needle In Out 3 buttons to adjust the x axis position Press the Needle Left Right 4 buttons to adjust the y axis position yaxis 1 In A j l X axis Out Right lt left 5 Using the step buttons in the Needle Up Down area 5 adjust the needle height as follows a Using the big step button WV lower the needle until it reaches a level of 2 3 mm 0 08 0 12 in above the vial or well b Using the small step button 4 slowly lower the needle until it reaches the bottom of the vial or well Tip For the ASC autosampler the O ring at the top of the needle holder rises a little when the needle reaches the bottom of the vial or well see Figure 246 Observe when the O r
114. LC 1000 instrument Description Pump Piston Seal Replacement Kit contains four spring energized piston seals and the piston seal tool Part number Quantity LC510 4 piston seals 1 per pump 1 extra Valve rotor seal LC228 8 rotor seals 2 per valve contains one rotor seal Column Out solvent line LC560 2 Waste In solvent line LC562 1 Autosampler needle ASC LC302 2 model Flow sensor filters contains LC542 4 flow sensor filters four flow sensor filters 2 per flow sensor EASY nLC Series Troubleshooting and Maintenance Guide 319 Ooo L Quick Reference Guides for Routine Maintenance This appendix contains a set of quick reference guides for the routine maintenance procedures that you can perform to keep the EASY nLC system in optimal working condition These guides are the condensed versions of the procedures in the Routine Maintenance chapter If you have replaced a PLF pump in the EASY nLC II instrument with a PLU pump follow the instructions in Maintaining the PLU Pump Quick Reference Guide on page 327 to maintain the new PLU pump Contents e Using nanoViper Fittings Quick Reference Guide e Maintaining the PLU Pump Quick Reference Guide e Maintaining the PLF Pump Quick Reference Guide e Maintaining the Rotary Valves Quick Reference Guide e Replacing a Damaged Autosampler Needle Thermo Scientific manual name 321 Using nanoViper Fittings Quick Reference Guide For the EASY nLC
115. Maintenance Scripts and Service Records Maintenance Scripts 2 Set up the parameters for this script as follows a Press the Parameters tab b Select the check boxes for the flow sensors A B or both that you want to either inspect or calibrate and inspect Note To calibrate and inspect the flow sensors this script performs a two point calibration as follows 1 Calibrates the 0 pL min point 2 Inspects and evaluates this first calibration point 3 Calibrates the 500 pL min point 4 Inspects and evaluates this second calibration point When you select the Inspection Only check box the script does not apply the new calibration Selecting the Fast Inspection option instead of the Exact Inspection option reduces the duration of the inspection and evaluation steps from 16 minutes to 5 minutes without reducing the accuracy of the calibration c Do one of the following e To check the accuracy of the current calibration only inspect the current calibration select the Inspection Only check box Note When you select the Inspection Only check box the program does not recalibrate the flow sensors e To calibrate and inspect the flow sensors and apply the new calibration clear the Inspection Only check box d Select the inspection duration as follows e When you want an estimate of the flow sensor accuracy select the Inspection Only check box and the Fast Inspection option e When you are recalibrating
116. Pressure 48 Flow Sensors 51 Flush Air 33 Isocratic Flow 38 Leaks 44 Maintenance 31 MS Connection 39 Purge Solvent 32 Reset Pressure Sensor 52 Sample Pickup 42 263 Valve Check 47 Valve Tune 50 seals piston description 8 replacing in the PLF model pump 62 in the PLU model pump 67 Secure Shell SSH network protocol 287 serial numbers device 106 service log 55 service repairs See EASY nLC II instrument or EASY nLC 1000 instrument service repairs sharp objects autosampler needle 278 sluggish system operation 202 software upgrading the touch screen application 128 296 solvent system components behind the right side panel 5 schematic EASY nLC 1000 system 317 EASY nLC II system 315 solvents checking levels 27 viscosity table 311 specifications performance 23 technical 24 spray unstable 196 SSH Connection Error message box 292 stator cleaning 75 replacing 79 support collar 148 support server IP address 289 swept volume extra 176 system overload 202 Thermo Scientific T technical specifications 24 technical support contacting xxiii Telnet window 266 temperature direct control 211 maintaining stable 284 Terminated label PC box 131 touch screen software description 18 upgrading 128 296 transport instructions 299 tray compartment accessing 2 troubleshooting autosampler 171 contact closure 172 identifying and locating leaks 218 instrument startup 172 network access 264 system blockage 249 turning off t
117. Remote Support Downloading the Latest Firmware File Downloading the Latest Firmware File If the EASY nLC instrument does not have the latest version of the touch screen application connect the instrument to the remote server and install the appropriate firmware files as described in the following procedure To install the latest firmware file 1 Ifthe instrument is not already connected to the remote server connect the instrument as follows a From the touch screen press Maintenance gt Support The Support page of the Maintenance menu appears b In the Connect to EASY nLC Support area press Connect If the EASY nLC instrument does not have the latest version of the touch screen application the Upgrade is Available dialog box appears Figure 256 shows the message for an instrument with firmware version 2 4 Figure 256 Firmware upgrade message x An upgrade is available The installed software can be upgraded to version 2 5 3032 Note the current version can be displayed by pressing the logo in the upper left corner of the application You can find the Software Release Notes by entering the EASY nLC User Zone from http jwww proxeon com Would you like to download an installable firmware file A dialog will be shown when the transfers of the instrument log files and optional firmware file are completed 2 Click Download When the file transfer is complete the Transfer of Log and Firmware Files
118. Scientific 6 Troubleshooting Troubleshooting a System Blockage To determine if the line that connects the mixing Tee to flow sensor A is blocked do the following a Disconnect the line from the outlet of flow sensor A b Depending on the back pressure do one of the following e Ifthe pressure is lt 20 bar the line that connects the mixing Tee to flow sensor A is blocked Replace it with a new solvent line e Ifthe pressure is gt 20 bar reconnect the line to flow sensor A and then go to step 8 for an EASY nLC 1000 instrument or step 9 for an EASY nLC II instrument To determine if the inline filter connected to the flow sensor outlet of the EASY nLC 1000 system is blocked do the following a Remove the inline filter from the flow sensor outlet b Depending on the back pressure do one of the following e Ifthe pressure is lt 20 bar the inline filter is blocked Replace it with a new filter e Ifthe pressure is gt 20 bar reconnect the inline filter to flow sensor A and then go to step 9 To determine if flow sensor A is blocked do the following a Disconnect the solvent line from the flow sensor A inlet b Depending on the back pressure for flow sensor A do one of the following e If the pressure is lt 20 bar flow sensor A is blocked Replace it with a new flow sensor as described in Replacing a Flow Sensor on page 92 e Ifthe pressure is gt 20 bar reconnect the line to flow sensor A and the
119. Support for the EASY nLC instrument Web site Www proxeon com E mail North and South America us customer support analyze thermofisher com Other continents eu techsupport cmf thermofisher com Address Thermo Fisher Scientific Edisonvej 4 DK 5000 Odense C Find software updates and utilities to download at mssupport thermo com To order consumable and spare parts for the EASY nLC instrument For the EASY nLC 1000 instrument go to www proxeon com productrange nano_ Ic_easy nlc_1000 accessories_spares index html For the EASY nLC II instrument go to www proxeon com productrange nano_lIc accessories spares index html Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide xxiii ee i Introduction The EASY nLC instrument see Figure 1 consists of a high pressure dual pump chromatography system that provides split free flows down to the low nanoLiter min range an automated sampling system and a built in computer with a touch screen interface for instrument control and troubleshooting The automated sampling system includes a tray compartment that can hold standard sample vials and microtiter plates an XYZ robot that moves to the sample location and a syringe pump that draws only the user specified sample volume The EASY nLC II instrument operates in the high performance pressure range of 1 to 300 bar and the EASY nLC 1000 instrument operates in the ultra high performance pressure range of 1 to 1000 bar
120. V 009800 run the Valve Tune script e For valve serial number gt V 009800 replace the valve 90 IC_DEVICE_ A device type operation Contact Thermo Fisher UNSUPPORTED_ was attempted on a Scientific OPERATION device instance that doesn t support it 92 IC_BUSINESSLOGIC_ Gradient interrupted by Pump solvent volume No action is necessary REFILL_DURING_ pump refill warning too small for next This is just a warning that GRADIENT gradient step the pumps were refilled during gradient processing 93 IC_SCRIPT_AUTO_ Maintenance script Probably because a Check for air and leaks in ABORT self abort pump ran out of the subsystem solvent while See Troubleshooting the building maintaining Results of the System Leak pressure Test on page 221 310 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific es i Mobile Phase Viscosity Table 31 lists the viscosities for two solvent mobile phases consisting of a water methanol mixture or a water acetonitrile mixture Table 31 Mobile phase viscosity at 25 C for reversed phase gradients nas cP Mobile phase v organic water Methanol Acetonitrile 0 0 89 0 89 10 1 18 1 01 20 1 40 0 98 30 1 56 0 98 40 1 62 0 89 50 1 62 0 82 60 1 54 0 72 70 1 36 0 59 80 1 12 0 52 C 4G 100 0 56 0 35 Reference D P Herman A H Billiet and L de Galan Journal of Chromatography 463 1989 1 Thermo Scientific EASY nLC Series Troubleshooting a
121. _PATH file already exists 7 ERROR_IO_FILE_CAN Failed creating log Filesystem error Internal filesystem error NOT_WRITE LOG_FILE_NAME_ Replace the hard drive AND_PATH file not writable 8 ERROR_BATCH_BEIN It appears the current see issue description Close or reschedule the G_EDITED batch is being edited batch please close or reschedule it 9 ERROR_DEVICE_ Failed accessing device Missing the hardware Add the device to ACCESS_FAILED DEVICE_ID component driver the list on the Maintenance gt Devices page Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 303 A Error Codes Table 30 Error codes Sheet 2 of 8 No Issue code name Description Cause Solution 10 ERROR PREP_ Pump B unable to Pump unable to reach Check that solvent bottle is GRADIENT_ reach 90 A pressure set pressure during not empty and check for air PRESSURE_BUILD_ within 30 pL prep gradient and leaks in the system FAILED Please check for air leaks 11 ERROR PUMP_ stopped out of solvent Out of solvent Refill the solvent reservoir STOPPED_EMPTY bottle Check for air and leaks in the system 12 ERROR _PUMP_ stopped unexpectedly Pump hardware issue Check the pump STOPPED _ unknown cause functionality UNEXPECTEDLY 26 ERROR_QUEUE_ Current queue element is Trying to execute ajob Close or reschedule the ELEMENT_BEING_ being edited stopping currently being edited batch EDITED 27 ERROR PBUS_IN_
122. a new Column Out line No Then rerun the system leak test page 218 Service valve S page 74 Then rerun the system leak test Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 227 6 Troubleshooting Troubleshooting the Results of the System Leak Test Figure 217 shows the path between valves S and W for the EASY nLC 1000 instrument Figure 217 Solvent path between valve S and valve W Valve S Valve W Blind fitting connected to port 1 Column Out Waste In line line Leak test union To locate and remedy a leak in valve S or valve W or in the solvent path between these valves 1 Make sure that the connections to the leak test union are secure as follows a Depending on the instrument model do the following e For the EASY nLC II instrument hand tighten the PEEK fittings e For the EASY nLC 1000 instrument do the following i Disconnect the nanoViper fittings from the union ii Reinsert the nanoViper fittings into the stainless steel union Then slowly tighten the fittings until you feel resistance iii Tighten both fittings by an additional 45 degrees 1 8 turn Do not tighten the fittings by more than 90 degrees 1 4 turn For more information see Using nanoViper Fittings on page 83 Note The connections to the leak test union are one of the most common sources of leaks for the system leak test b For leak testing set up and st
123. acquisition The mass spectrometer remains in the Waiting for Contact Closure state until it receives the start signal The EASY nLC instrument sends the start signal when the run gradient step begins Figure 201 Waiting for Contact Closure error 5 velos 10 32 134 20 Remote Desktop X Thermo Xcalibur Sequence Setup gh_110414132511 Open File Edit Change Actions Yiew GoTo Help fay ele Des el TBO 10 eE gt maa 2 ample Typ FileName Sample ID Path Inst Meth Proc Meth Position Status Acquisition Queue Unknown BlankUDGI A2 C Xcalibur D C Xcalibur m Al Run Manager Unknown BlankUDG2 amp 3 C Xcalibur D C Xcaliburkm A2 Control Device Started Unknown BlankUDG3 A4 C Xcaliburs D C Xcalibursm A2 Sequence C Xcalibur data PN 2011 Apr Unknown 45 C Xcalibur D C Xcalibur m A2 Sample Name Working On Seq Row 1 Position A1 Raw File C Xcalibur D ata PN 2011 April Inst Method C Xcalibur methods PN metl s LTQ Velos MS Waiting for Contact Closure lt admin 10 32 134 15 LC 000550 Run gradient Mass spectrometer state EASY nLC method step Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 193 6 Troubleshooting Troubleshooting Tips Carryover Table 18 lists the possible causes of carryover Table 18 Possible causes of carryover Symptom Possible cause Action Carryover from one run to the Incomplete elution of peptides Add extra time to the end of the gradie
124. ad for a PLF model pump Stainless steel fitting connected to an internally threaded pump head Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 63 4 Routine Maintenance Maintaining the Syringe Pumps 6 Using a 13 mm open ended wrench remove the pump head see Figure 50 Figure 50 Removing the pump head Pump head PLF model pump 7 Clean the piston as follows a Place the piston in the fully extended position as follows i Turn on the instrument and log in as an administrator ii Press Home gt Overview iii Press the pump icon for the pump you want to control The Pump dialog box opens iv In the flow rate box enter the flow rate 300 pL min v In the volume box enter the dispense volume 140 pL vi Press Start b Soak a lint free tissue in methanol and then squeeze out the excess solvent c Clean the piston with the lint free tissue soaked in methanol and visually inspect the piston for any scratches Take care to avoid solvent drips as the pump printed circuit board can be damaged by exposure to solvents CAUTION The pump PCB is easily damaged by exposure to solvents d Return the piston to the fully retracted position as follows i In the Pump dialog box set the flow rate to 300 pL min and the dispense volume to 140 uL ii Press Start 8 When the piston is fully retracted close down the EASY nLC system and turn off the power see Closing Down the EASY
125. against the bottom of the receiving port EASY nLC Series Troubleshooting and Maintenance Guide 173 6 Troubleshooting Troubleshooting Tips Leaks that Cause Delayed Elution When there is a leak in the system upstream from flow sensor B part of the solvent stream from pump A exits the mixing Tee toward flow sensor B pushes solvent B back toward valve B and partly fills the solvent line between the mixing Tee and flow sensor B during the column equilibration and sample loading steps see Figure 181 When the gradient step begins instead of pushing solvent B into the mixing Tee pump B initially pushes this small volume of solvent A delaying the start of the actual gradient Figure 181 Negative flow of solvent B toward valve B during the column equilibration and sample loading steps Positive flow Mixing Tee Negative flow Flow sensor B ae a a A leak in valve B or the solvent line between valve B and flow sensor B can allow solvent A to enter the solvent path between the mixing Tee and valve B during the column equilibration and sample loading steps 174 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific Thermo Scientific 6 Troubleshooting Troubleshooting Tips Figure 182 shows the flow and pressure traces for pump B for a system with a leak upstream of flow sensor B The measured flow rate is negative rather than 0 nL min during the column equilibration and sample loading steps Fi
126. age 254 installing in the EASY nLC II instrument 135 monitor cable internal 114 monitor replacing service repair 113 motor cable 165 168 MS Connection script 39 nanoViper fittings EASY nLC 1000 solvent lines 316 using 83 323 needle arm 135 needle guide white plastic 115 116 needle position calibrating 278 286 needle autosampler 14 network access troubleshooting 264 Thermo Scientific Index P parts common replacement 318 password initial 20 P Bus communication cables connecting to cooler PCB 132 139 connecting to pump 166 connecting to pump PCB 121 168 connecting to XYZ axis PCB 132 disconnecting from cooler PCB 129 137 disconnecting from pump 152 163 disconnecting from pump PCB 118 158 167 disconnecting from XYZ axis PCB 129 inserting P Bus connector 131 PCBs connected to 132 P Bus connector 131 PC box cables connected to 108 replacing service repair 108 Terminated label 131 PCB shield 164 166 performance specifications 23 pink autosampler icon 201 pump icons 199 valve icon 200 piston seals function 8 replacing in the PLF model pump 62 in the PLU model pump 67 plate formats creating 275 deleting 277 selecting 274 plates calibrating 280 plug EASY nLC II instrument 218 port 22 firewall setup for 287 288 Power Down button 22 power turning off 22 preparing autosampler for the calibration routines 278 system for daily use 27 pressure sensor cable 152 163 166 pressure sensors communi
127. age 296 c Ifthe Upgrade is Available dialog box appears do one of the following e Upgrade the software as described in Downloading the Latest Firmware File on page 296 or e Click Skip The Message to Support Team dialog box appears 290 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 8 Remote Support Connecting the EASY nLC Instrument to the Support Server d Select a support region provide valid contact information and describe the problem that you are experiencing Figure 253 Message to Support Team dialog box Message to support team P Support region Name Email address Phone number Enter message ERS Accept Cancel Note When you place the cursor in the Phone Number box the program prompts you for a country code and phone number Tip When you place the cursor in the Email Address box the program prompts you to enter an address in this format name company com To enter the symbol press Shift The numbers row changes to the symbols row Then press e Press Accept After you enter the requested information the Status box on the right side of the Support page should change from Not Connected to Connected Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 291 8 Remote Support Connecting the EASY nLC Instrument to the Support Server f Depending on the connection status d
128. aged pump and replace the pressure sensor 2 Disconnect the tubing from the inlet and outlet ports of the flow sensors 3 Replace the flow sensor bracket with the new flow sensor bracket supplied in the Pump Mounting Kit as follows a Using a 2 5 mm hex wrench remove the two screws that secure the flow sensor bracket to the instrument panel see Figure 171 b Set the two 2 5 mm screws aside for reuse when you secure the new flow sensor bracket Figure 171 Removing the two screws that secure the flow sensor bracket to the panel 2 5 mm hex wrench c Using a 2 0 mm hex wrench or ball driver remove the four screws that secure the two flow sensors to the flow sensor bracket see Figure 172 d Set the 2 0 mm hex screws aside for reuse when you secure the flow sensors to the new flow sensor bracket 160 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 5 Field Service Maintenance Replacing a Pump Figure 172 shows the four 2 0 mm hex screws that secure the flow sensors to the flow sensor bracket and the original flow sensor bracket Figure 173 shows the flow sensors removed from the flow sensor bracket The flow sensor cables are still connected to the instrument Figure 172 Screws that secure the flow sensors to the bracket Two screws per flow sensor e Using a 2 5 mm hex wrench and the screws that you set aside when you removed the flow sensors from the original flow sensor bracket conne
129. aining the PLU Pump Quick Reference Guide 0 327 Maintaining the PLF Pump Quick Reference Guide 0 329 Maintaining the Rotary Valves Quick Reference Guide 331 Replacing a Damaged Autosampler Needle 0000000 00048 333 ndek reyo a R ei eapea eae wets aerate wine eee AE Deas 335 EASY nLC Series Troubleshooting and Maintenance Guide xvii Oo Y Preface This guide describes the maintenance and troubleshooting procedures required to maintain the EASY nLC instrument in optimal working condition The hardware maintenance procedures are divided into two chapters Routine Maintenance and Advanced Maintenance The Routine Maintenance chapter contains common maintenance procedures that you can perform to keep the EASY nLC instrument in optimal working condition The Advanced Maintenance chapter contains hardware repair procedures that can only be performed by a Thermo Fisher Scientific field service engineer or a skilled technician with equivalent training For information about setting up the EASY nLC instrument and running chromatographic separations refer to the EASY nLC Series Getting Started Guide Contents e Related Documentation e Downloading Manuals from the Customer Manuals Web Site e Safety and Special Notices Contacting Us To suggest changes to documentation or to Help If you are viewing a PDF file of this manual online complete a brief survey about this d
130. aintenance Replacing the Autosampler Needle To install a new autosampler needle 1 Mount the new autosampler needle in the z axis holder Check that the small spring is situated between the PEEK nut and the plastic stop 2 Do one of the following e For the ASC autosampler go to step step 3 e For the ASA autosampler go to step step 4 3 For the ASC autosampler do the following a Guide the end of the needle that connects to valve S through the large hole in the metal plate that separates the tray compartment from the solvent system compartment b Install the black needle holder in the side plate 4 For the ASA autosampler slide the valve end of the tubing through the small plastic holder on the back of the touch screen monitor see Figure 79 Figure 79 Holder on the back of the touch screen monitor Holder 5 Connect the needle to port 1 of valve S as follows a Slide the provided blue sleeve and metal fittings onto the tubing b To ensure a zero dead volume connection hold the blue sleeve and the tubing firmly against the bottom of the valve port and then tighten the fitting with a 1 4 in open ended wrench 6 Recalibrate the needle position as described in Chapter 7 Calibrating the Autosampler s XYZ Robot 7 Run 2 iterations of the Purge Solvent script for Pump S For information about running the Purge Solvent script see Prepare Purge Solvent on page 32 8 Run the Flush Air
131. ake sure the pump head is filled with solvent during the entire retraction of the piston A 3 Using a 1 4 in open ended wrench reconnect the tubing to the pump head CAUTION Be careful not to spill solvent onto the printed circuit board behind the LED panel Preparing the Instrument for Operation 1 Reinstall the right panel 2 Reset the usage counter for the pump as follows a Press Maintenance gt Devices b Select the pump from the list of devices c Press the Summary tab d Press Reset The value in the Intermediate Volume box resets to 0 3 To draw fresh solvent through the solvent lines and to remove air do the following a Open the Parameter page for the Purge Solvent script b In the Purge Iterations box enter 10 c Select the check box for the appropriate pump d Press Start and wait for the system to perform 10 purge cycles 4 To flush air from the pump head do the following a In the Name category on the Maintenance gt Scripts page select Flush Air b Press the Parameters tab c In the Flush Threshold uL box enter 10 d Press Start e Wait for the script to finish 5 Run the Leaks script for the pump as follows a On the Maintenance gt Scripts page select Test in the Category list and then select Leaks in the Name list b Press the Parameters tab c Select the check box for the pump with the new seal d Press Start 6 When the instrument is leak tight it is ready
132. al filled with solvent A e Microtiter plate if you are using a microtiter plate format To troubleshoot the autosampler aspiration of sample into the needle 1 Make sure that the W3 wash bottle contains sufficient solvent 2 Use the Flush Air script to remove air from pump S as follows a b Thermo Scientific Press Maintenance gt Scripts In the Category list select Prepare In the Name list select Flush Air Press the Parameters tab Select the Flush Pump S check box For Flush Threshold pL enter 10 for the PLF model pump or 12 for the PLU model pump see Figure 231 Figure 231 Flush Air script Thermo inlet MMe sta ol o geenmtelolam Maintenance Eeron tile SCIENTIFIC f Log Book Prepare X Flush air Support Desired flow Pressure o Description Parameters Output Devices o w Parameter Flow nl min o a Flush pump A Flush pump B Flush pump S Time min a graph Flush threshold pl Job 00 00 00 6 May 2011 11 15 admin Schedule Exit EASY nLC Series Troubleshooting and Maintenance Guide 261 6 Troubleshooting Troubleshooting the Autosampler Aspiration and Calibration g Press Start The script runs until the flush threshold reaches the specified value or one of these error messages appears on the Output page e Aborting unable to build pressure e Aborting check solvent level 3 Depen
133. ance 1 eet eee eens 28 Yeatly Mamnenanie hn Fhe dentara E n Ree ee he e OPA ees 29 Field Servi REGAN occu cater eiuia i a ee DENTE anwatees 29 Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide xiii Contents Chapter3 Maintenance Scripts and Service Records 0 0eeeeeeeeeees 31 Maintenance Sctipts seai eas iiin i ytd a cay tas don sy bens dog dean aE gov 31 Prepare Purse Solvent eide ta t e eb iates Wale Ras aa 32 Prepare Flush Air acco ache ounce ai Sates ako as Ree ate ee aes 33 Prepare Precolumn Equilibration n os wee tes xaos eed a cee 34 Prepare Analytical Column Equilibration 00 000000 36 Prepare Isocratic Flow ans vawcade vous vary oon ver toen ces awa 38 Test MS Connection otc rit hoor he here Spee MEE ea 39 Test Sample Picktpin xen See Soe aie Men dp acta tees eae 42 E E oe das BG Sabie Pipes in Mee GRR ellie piel hn tallies tle 44 hest Valve Checks sis nest Asuna a ate Sh Sach te Sale meee ge See 47 Test Back Press re siii ayes 0 4 4 at ch ie ai aha aaa i A po aA ley 48 Test Autosampler Vorquesy wishes sabes wie ence nnr r rnrn 49 Test Pump Torgus s rrer esi nord es R A A e R A 50 Calibrate Valve T ney ninri yea yE E ERER E EE oe E 50 Calibrate Flow Sensons ss isu nha gle acne ale nerneur 51 Calibrate Reset Pressure Sensor o n uiesewntoe tigen eh platen et 3 52 Calibrate Direct Infusion os snu unaua eeuu u rnana
134. ance Guide 257 6 Troubleshooting Troubleshooting a System Blockage To troubleshoot a system blockage when only the Test Solvent B test fails 1 To determine if the blockage is in the mixing Tee do the following a Make sure that the Column Out line is connected to the Waste In line with a union b Remove the solvent line from the mixing Tee that connects it to flow sensor B c Repeat the Back Pressure script with the Test Solvent B check box selected d Depending on the test results do one of the following If Test solvent B passes clean the mixing Tee and if necessary replace it If Test solvent B fails go to step 2 2 To determine if the blockage is in the solvent line that connects the B flow path to the mixing Tee do the following a Disconnect the solvent line from the outlet of flow sensor B b Place valve B in position 1 6 as follows i Press Home gt Overview ii Press the valve B icon iii In the Valve B dialog box press 1 6 c Set pump B to deliver 2 pL min as follows i Press the pump B icon ii In the flow rate box type 2 pL min iii Press Start iv Leave the dialog box open For more information about the direct controls for the pump see Using the Pump Controls on page 205 d Monitor the Pressure readback box for pump B Then depending on the back pressure do one of the following e Ifthe pressure is lt 20 bar the solvent line is blocked For the EASY nLC II ins
135. ance Tasks e Closing Down the EASY nLC Instrument Description of the Touch Screen Monitor The instrument monitor is a transparent layer of glass that detects finger pressure even while wearing gloves and sends the corresponding commands to the computer see Figure 18 Figure 18 Touch screen controls ue Home gt Overview page 18 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 1 Introduction Touch Screen Application Instrument control from this touch screen application includes a method wizard that helps you create chromatographic methods a batch editor that helps you create and run sample batches injection sequences maintenance scripts and record keeping features and direct control of the valves pumps XYZ robot and tray temperature Note This guide describes how to log in to the instrument as an administrator how to use the maintenance scripts and record keeping features and how to exit the touch screen application in a controlled manner and turn off the instrument before performing maintenance procedures on the instrument s hardware components For information about the maintenance scripts and record keeping features see Chapter 3 Maintenance Scripts and Service Records For information about creating methods and sample batches and running sample batches refer to the EASY nLC Series Getting Started Guide for the touch screen application The EASY nLC touch screen a
136. appropriate pump g Press Start The piston moves downward to the 140 pL position 2 Exit the EASY nLC 1000 system and turn off the power 3 Using a 2 Phillips head screwdriver with a quarter turn loosen the three captive screws that secure the right side panel to the instrument Then remove the panel CAUTION Wear powder free gloves when handling the wetted components of the LC system 4 Remove the used piston seal as follows a Using a 1 4 in open ended wrench remove the stainless steel tubing connected to the pump head Figure 2 Stainless steel fitting removed from the pump head Stainless steel tubing b Using a 3 mm hex wrench remove the two screws that secure the pump head to the pump body Figure 3 Pump head removed from the pump body c Clean the alep portion of the piston with a lint free tissue soaked in LC MS grade methanol and visually inspect the piston for scratches Ensure that no solvent runs into the pump d Remove the old seal from the pump head with great care Do not use any metal tools as they can scratch the inside of the pump head and generate leaks Installing a New Piston Seal 1 With the spring side of the piston seal spring facing away from the piston seal tool mount the new piston seal onto the piston seal tool 2 Using a pipette remove the air from the piston seal spring by carefully filling the rim of the piston seal with methanol Figure 4 Filling the pisto
137. art the Analytical Column Equilibration script as follows i On the Maintenance gt Scripts page select Prepare in the Category list and then select Analytical Col Equilibration in the Name list ii Press the Parameters tab iii In the Volume pL box enter 140 This volume should be sufficient to keep the solvent flow on during this troubleshooting procedure iv Leave the Flow box pL min box empty so that pump A operates at the set pressure 228 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 6 Troubleshooting Troubleshooting the Results of the System Leak Test v Inthe Max Pressure bar box enter 280 bar for the EASY nLC II instrument or 980 bar for the EASY nLC 1000 instrument vi Press Start The instrument places the valves in the following positions and pump A begins pumping solvent through flow sensor A e Valve A 1 6 e Valve S 1 2 e Valve B Center e Valve W 1 2 Figure 218 shows the solvent system schematic on the Home gt Overview page Use this page to monitor the readback for flow sensor A and to access the valve controls For information about changing the valve positions see Using the Valve Controls on page 204 Figure 218 Home gt Overview page showing the readback for flow sensor A Readback Press this icon to for flow sensor A with open the controls a flow lt 100 nL min for valve W Thermo Mom EET Method Editor Maintenance Configur4 SCIENTIFIC 979 8 bar ac
138. ate e When you would like to replace the needle e For problems with sample pickup for example the needle is not placed in the center of the well or not all of the liquid is picked up from one or more of the vials e For problems with the wash waste bottles for example the needle is not placed in the center of the bottle Replacing the Autosampler Adapter Plate The EASY nLC system ships with the vial adapter plate installed The microtiter adapter plate comes in the accessory kit To load microtiter microwell plates into the tray compartment you must replace the vial adapter plate with the microtiter adapter plate The vial adapter plate holds 48 vials 6 extra vials The microtiter adapter plate holds one 96 well plate or one 384 well plate Follow the procedure for the autosampler model in your EASY nLC system to switch between the two adapter plates as necessary e Replacing the Adapter Plate in the ASC Model Autosampler on page 271 e Replacing the Adapter Plate in the ASA Model Autosampler on page 272 270 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 7 Calibrating the Autosampler s XYZ Robot Replacing the Autosampler Adapter Plate Replacing the Adapter Plate in the ASC Model Autosampler The ASC model is the current autosampler model for both the EASY nLC II and the EASY nLC 1000 systems To replace the adapter plate in the ASC model autosampler 1 Press Home gt Overview
139. ator 1 Close down the EASY nLC system and then turn off the power to the instrument see Closing Down the EASY nLC Instrument on page 21 Using a 2 Phillips screwdriver loosen the three quarter turn screws that secure the panel to the right side the EASY nLC instrument and then remove the panel Disconnect the solvent lines from the valve as follows e Use a 1 4 in open ended wrench to remove stainless steel fittings e Use the black nanoViper knurled nut to remove nanoViper fittings Using a 9 64 in L hex wrench remove the two hex screws that secure the stator to the valve driver and then pull the stator away from the valve driver Mount the new stator onto the valve driver 6 Using a 9 64 in L hex wrench tighten the two hex screws a little 10 11 at a time by shifting from one screw to the other and back again until the screws are evenly torqued Reconnect the solvent lines to the valve as follows e Use a 1 4 in open ended wrench to tighten stainless steel fittings e Use the black nanoViper knurled nut to tighten nanoViper fittings Reconnect the right side panel to the instrument Tighten the three screws with a 2 Phillips screwdriver Turn on the EASY nLC instrument and log in as an administrator If you replaced the stator in Valve A or B run the Flush Air script for the valve with the replacement stator If you replaced the stator on Valve A or B run the Leaks script for t
140. autosampler s XYZ arm is blocked The error repeats without any visible reason Remove the obstacle and try again You might need to calibrate the autosampler for more precise penetration of the microtiter plate or the wash bottles For information about calibrating the autosampler see Chapter 7 Calibrating the Autosampler s XYZ Robot Contact Thermo Fisher Scientific e us customer support analyze thermofisher com e eu techsupport cms thermofisher com A server warning message about the plate format for the autosampler configuration pops up when you submit a batch Thermo Scientific The plate format has been deleted from the system configuration For information about deleting a plate format refer to the EASY nLC Series Getting Started Guide If you want to run the batch you must create the plate format again For information about selecting creating and deleting plate formats see Managing Plate Formats on page 274 EASY nLC Series Troubleshooting and Maintenance Guide 171 6 Troubleshooting Troubleshooting Tips Contact Closure Problems Table 10 contains troubleshooting tips for contact closure problems Table 10 Contact closure troubleshooting tips Symptom The mass spectrometer is waiting for a contact closure signal setup The EASY nLC instrument is waiting for a ready signal from the mass spectrometer Instrument Startup Problems Possible causes Incorrect
141. autosampler into an instrument that previously held the ASA model on page 130 To remove the ASA autosampler from the EASY nLC II instrument 1 Upgrade the touch screen software to the latest version as described in Downloading the Latest Firmware File on page 296 Note Read the release notes before you upgrade to ensure that you have the most recent version of the HPLC software 2 Press Maintenance gt Devices and remove the old autosampler from the device list 3 Close down the EASY nLC instrument see Closing Down the EASY nLC Instrument on page 21 Then unplug the power cable 4 Remove the computer box as described in To remove the computer box on page 108 Note Removing the computer box requires the removal of the left side panel and the back panel 128 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 5 Field Service Maintenance Upgrading from an ASA Autosampler to an ASC Autosampler 5 Using a 2 Phillips head screwdriver with a quarter turn loosen the three captive screws that secure the right side panel to the instrument housing Then remove the panel 6 Remove the monitor as described in To remove the monitor on page 113 7 Using a 2 5 L hex wrench or ball driver loosen valve W see Figure 123 Figure 123 Loosened valve W 8 Disconnect the two P Bus communication cables from the cooler PCB and XYZ axis PCB on the right side of the instrument
142. avoid damage by overtightening To use a nanoViper fitting 1 Insert the nanoViper fitting into the target port and slowly rotate the screw clockwise until you feel resistance 2 Using the black knurled tightening tool turn the screw clockwise to an angle between 0 and 45 degrees 1 8 turn 3 Run the Leaks script for the system as described in Running a System Leak Test on page 218 When the leak test ends the system is at atmospheric pressure IMPORTANT To extend the lifetime of the nanoViper fittings open and close connections at atmospheric system pressures only Opening and closing connections at high system pressures can reduce the lifetime of the fitting system 4 Ifthe Leaks script fails because the new connection is not leak tight use the black knurled tightening tool to turn the screw by as much as an additional 45 degrees Do not turn the screw beyond an angle of 90 degrees from where you felt the initial resistance IMPORTANT To prevent damage to the sealing surface of the nanoViper fitting take care not to overtighten the nanoViper fitting Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 83 4 Routine Maintenance Replacing the Autosampler Needle Replacing the Autosampler Needle Replacing the autosampler needle requires these tools and materials Tools Parts and materials e 1 4 in open ended wrench e Powder free safety gloves e 2 Phillips head screwdriver e ASA a
143. bing and from the inlet and outlet of the flow sensor Figure 83 shows the flow sensors mounted behind the bracket The flow direction is labeled on the bracket Figure 83 Flow sensors nanoViper fitting 5 gt hlnefilter OJ Inlet end Outlet end Inline filter nanoViper fitting 4 Using an 8 mm open ended wrench remove the inline filters from both ends of the flow sensor see Figure 83 Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 93 4 Routine Maintenance Replacing a Flow Sensor 5 Remove the black cable from the flow sensor see Figure 84 Figure 84 Removing the cable from the flow sensor 6 Using an 2 5 mm hex wrench remove the two screws that secure the flow sensor to the bracket see Figure 85 Figure 85 Removing the screws attaching the flow sensor to the bracket Location of screws that secure flow sensor A 94 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 4 Routine Maintenance Replacing a Flow Sensor 7 Remove the flow sensor see Figure 86 Figure 86 Removing the flow sensor To install an EASY nLC 1000 flow sensor 1 Insert the new flow sensor behind the bracket 2 Insert the two screws Figure 85 on page 94 that secure the flow sensor to the bracket and tighten the screws with a 2 5 mm hex wrench 3 Connect the black cable to the flow sensor
144. box c Select the ASC autosampler from the list and then press Accept d Verify that the ASC autosampler has been added to the Devices list i Select the ASC autosampler in the Devices list ii Check the information on the About page For more information about adding devices to the Devices list see Managing the Devices List on page 105 Calibrate the autosampler as described in Chapter 7 EASY nLC Series Troubleshooting and Maintenance Guide 127 5 Field Service Maintenance Upgrading from an ASA Autosampler to an ASC Autosampler Upgrading from an ASA Autosampler to an ASC Autosampler If your EASY nLC II instrument has an ASA autosampler that is irreversibly damaged you must upgrade to an ASC autosampler because the ASA autosampler is no longer available CAUTION Wear powder free gloves and safety glasses when handling parts of the LC system that come into contact with solvents Upgrading the instrument by replacing an ASA autosampler with an ASC autosampler requires these items Tools Parts and materials e 4 mm ballpoint hex wrench e Powder free gloves e 2 mm L hex wrench or ball driver e M5 x16 MC hex screw e 2 5 mm L hex wrench or ball driver e ASC autosampler P N LC301 e Torx T 10 wrench e 2 Phillips screwdriver e Tweezers To replace the ASA autosampler with an ASC autosampler follow these steps 1 To remove the ASA autosampler from the EASY nLC II instrument on this page 2 To install the ASC
145. bracket see Figure 168 Then set the L bracket and the two 2 mm hex screws aside for reuse when you mount the new pressure sensor Figure 168 Pressure sensor L bracket screws 2 5 mm ball driver Screws that secure the pressure sensor to the L bracket Screws that secure the L bracket to the instrument panel Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 157 5 Field Service Maintenance Replacing a Pump c Disconnect the P Bus communication cables from the pump actuator see Figure 169 Figure 169 Disconnected P Bus communication cable communication cable d Using a3 mm ball driver or L hex wrench remove the four screws that secure the pump to the instrument panel see Figure 170 e Set the four 3 mm screws aside for reuse in step 8a on page 159 Figure 170 Removing the four screws that secure the pump to the instrument panel 3 mm driver 6 Install the new pressure sensor as follows a Usinga2 0 mm L hex wrench or ball driver and the two 2 0 mm hex screws that you set aside when you disconnected the original pressure sensor from the L bracket connect the new pressure sensor to the L bracket b Using a 2 5 mm L hex wrench or ball driver and the two 2 5 mm hex screws that you set aside when you removed the L bracket from the instrument panel reconnect the L bracket to the instrument panel 7 Ifyou are replacing pump S in an early version of the EASY nLC II instrument
146. c 4 Routine Maintenance Replacing the Hard Drive To remove the hard drive 1 Close down the EASY nLC instrument and then turn off the power to the instrument see Closing Down the EASY nLC Instrument on page 21 2 Using a 2 5 mm hex wrench loosen the two screws in the hard drive drawer see Figure 92 Figure 92 Screws that secure the hard drive drawer obsolete computer box shown Two screws that secure the hard drive drawer 3 Pull out the hard drive drawer as shown in Figure 93 Figure 93 Pulling out the hard drive drawer Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 101 Routine Maintenance Replacing the Hard Drive 4 Using a Torx T 10 wrench remove the four screws underneath the hard drive drawer that secure the hard drive to the drawer see Figure 94 Figure 94 Removing the screws underneath the hard drive drawer 5 Unplug the hard drive from the IDE cable see Figure 95 Figure 95 Hard drive unplugged from the IDE cable IDE cable The flat IDE cable is connected to the hard disk The text on the hard disk is upside down and the pins on the hard drive fit in the same place on the flat cable connector 102 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 4 Routine Maintenance Replacing the Hard Drive Tip If the IDE cable plug is difficult to access remove the brass rod that holds the cable down see Figure 96 Use a Tor
147. c EASY nLC Series Troubleshooting and Maintenance Guide 225 6 Troubleshooting Troubleshooting the Results of the System Leak Test Locating a Leak In or Between Valve S and Valve W If the system leak test returns the following message see Table 24 on page 221 follow the workflow in Figure 215 and Figure 216 or the procedure on pages 228 234 to locate and remedy the leak Check from valve S to valve W including valve W for leaks Figure 215 Workflow for locating leaks in the solvent path between valves S and W or in these valves Part 1 System leak test fails and returns the following message Check from valve S to valve W including valve W for leaks For the EASY nLC 1000 redo the nanoViper fitting connections to the leak test union step 1a on page 228 For the EASY nLC Il hand tighten the fittings connected to the leak test union Start the Analytical Column Equilibration script step 1b on page 228 Readback for flow sensor A gt 100 nL min No Done Using a 1 4 in wrench tighten the blind stainless steel fitting in port 1 of valve W step 2a on page 230 Readback for flow sensor A gt 100 nL min Yes Set valve W to the Center position step 3a on page 238 No Readback for flow sensor A gt 100 nL min Service valve W page 74 Then rerun the system leak test page 218 Yes Go to Figure 216 on the next page 226 EASY nLC Series Troubleshooting an
148. c EASY nLC Series Troubleshooting and Maintenance Guide 29 ee Maintenance Scripts and Service Records To familiarize yourself with the maintenance scripts and record keeping features provided by the EASY nLC software review this chapter Contents e Maintenance Scripts e Keeping Service Records e Checking and Resetting the Device Usage Counters Maintenance Scripts The EASY nLC touch screen software includes a variety of built in maintenance scripts that help you prepare the instrument for use and troubleshoot instrument problems To select a maintenance script 1 On the touch screen press Maintenance gt Scripts 2 Select a category and then the script for the specific operation you want You can also schedule some of the scripts for execution using the job queue Each of the following topics combines the category with the actual script name Prepare category Test category Calibrate category e Prepare Purge Solvent e Test MS Connection e Calibrate Valve Tune e Prepare Flush Air e Test Sample Pickup e Calibrate Flow Sensors e Prepare Precolumn Equilibration e Test Leaks e Calibrate Reset Pressure Sensor e Prepare Analytical Column Equilibration Test Valve Check e Calibrate Direct Infusion e Prepare Isocratic Flow e Test Back Pressure e Test Autosampler Torque e Test Pump Torque Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 3
149. c tool remove the old seal from the pump head see Figure 60 CAUTION Avoid using a metal tool to remove the piston seal from the pump head as metal can scratch the inside of the pump head and generate leaks Figure 60 Worn piston seal installed in the pump head PLU model pump 6 Install the new piston seal as follows a With the spring side of the piston seal facing upward mount the new piston seal onto the piston seal tool see Figure 61 Figure 61 Piston seal mounted on the piston seal tool PLU model pump Piston seal spring b Using a pipette fill the rim of the piston seal with 100 LC MS grade methanol to remove the air from the piston seal spring see Figure 62 Figure 62 Filling the piston seal with methanol PLU model pump Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 69 4 Routine Maintenance Maintaining the Syringe Pumps c Place the pump head on the guide rods and evenly press it all the way down against the piston seal tool to insert the seal into the pump head see Figure 63 Figure 63 Inserting the piston seal into the pump head PLU model pump d Gently pull the pump head off of the guide rods see Figure 64 Figure 64 Removing the pump head from the piston seal tool PLU model pump 7 Using the two screws and a 3 mm L hex wrench reconnect the pump head to the pump body Do not connect the stainless steel tubing to the pump head 8 Go
150. cation cable 119 121 167 description 10 grounding cable connecting to PCB service repair 118 121 168 replacing EASY nLC 1000 instrument routine maintenance 90 EASY nLC II instrument service repair 117 EASY nLC Series Troubleshooting and Maintenance Guide 337 Index Q pump direct control 205 manual control 71 205 PLF EASY nLC II instrument description 8 maintaining 62 replacing service repair 154 PLU EASY nLC 1000 instrument description 8 maintaining 67 replacing service repair 150 volume pumped usage counter 56 Pump dialog box 206 Pump Mounting Kit 154 160 pump PCB replacing service repair EASY nLC 1000 instrument 163 166 EASY nLC II instrument 167 168 Purge Solvent script 32 Q quick reference guides autosampler needle replacing 333 EASY nLC 1000 pump maintaining 327 EASY nLC II pump maintaining 329 nanoViper fittings using 323 valves maintaining 331 R regulatory compliance iii remote support 287 replacement parts common 318 Reset Pressure Sensor script 52 rotary valves See valves rotor seal replacing 77 S safety calibrating the autosampler 278 precautions for the EASY nLC instrument xxi standards EMC vii safety standards iii safety standards EMC v sample pickup check 263 Pickup script 42 sample loop replacing 89 schedule maintenance 27 338 EASY nLC Series Troubleshooting and Maintenance Guide scripts Analytical Col Equilibration 36 Autosampler Torque 49 Back
151. cation cables 7 Calibrate the flow sensor by following the automated script described in Calibrate Flow Sensors on page 51 168 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific Troubleshooting To troubleshoot problems with the EASY nLC instrument follow the troubleshooting tips and procedures in this chapter Contents e Troubleshooting Tips e Using the Direct Controls for Troubleshooting and Maintenance e Troubleshooting a Pump that Fails the Flush Air Script e Troubleshooting a Pump that Fails the Leaks Script e Running a System Leak Test e Troubleshooting the Results of the System Leak Test e Identifying a Leaking Check Valve e Troubleshooting a System Blockage e Troubleshooting the Autosampler Aspiration and Calibration e Checking Sample Pickup e Troubleshooting Communication Problems e Verifying that the LC MS System Is Properly Grounded Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 169 6 Troubleshooting Troubleshooting Tips Troubleshooting Tips To troubleshoot problems with the EASY nLC instrument see these topics e Autosampler Problems on page 171 e Contact Closure Problems on page 172 e Instrument Startup Problems on page 172 e Delayed Elution on page 173 e Excessive Duration or Higher Pressure for the Column Equilibration and Sample Loading Steps on page 178 e System Reaches Its Maximum Pressure During t
152. ced Clogged syst Run the Back P ipt fi A Whaikonooi gaien ogged system un the Back Pressure script for pump maximum pressure for one or both If the test fails go to Troubleshooting a of these steps the duration of these System Blockage on page 249 steps is longer Figure 185 and Figure 186 show the flow rate and pressure traces for pump A for a normal run and a run with a partially clogged column respectively The flow rate for a run with a clogged column is lower than the flow rate for a normal run The lower flow rate increases the duration of the column equilibration and sample loading steps Note The specifications for the precolumns used to acquire the data in Figure 185 and Figure 186 are as follows e 2cm length e 100 um ID e 5 pm particle size e C18 178 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific Thermo Scientific 6 Troubleshooting Troubleshooting Tips Figure 185 Normal run SCIENTIFIC Overview Pump A Desired flow Real flow Pressure 110 000 07 Flow nl min ueq aunssasg job orot Sample 00 30 49 Gradiem 20 00 19 jul 2013 11 12 admin 150 175 200 225 250 275 300 Time min Sample loading step 10 L min Precolumn equilibration step 10 L min Figure 186 Run with a partially blocked column SCIENTIFIC Overview Pump A Graphs Desired flow Real flow Pressure Queue Flow nl min a aunssesg
153. code 57 ADDRESS URN_CODE for asr codes 65 ERROR_ needle down failed Could not move needle see above MANIPULATOR_ AUTOSAMPLER RET down see code 57 for NEEDLE_DOWN URN_CODE asr codes 66 ERROR_ needle up failed Could not move needle see above MANIPULATOR_ AUTOSAMPLER _ up see code 57 for asr NEEDLE_UP RETURN_CODE codes Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 307 A Error Codes Table 30 Error codes Sheet 6 of 8 No Issue code name Description Cause Solution 69 ERROR VALVE SET COMPONENT_ID Valve unable to switch Clean the rotor seal and the POSITION setPosition failed position stator Replace the rotor Unrecognized position seal if necessary POSITION COMPONENT _ID If the problem persists do setPosition failed the following Problems switching to e For valve serial number position POSITION lt V 009800 run the COMPONENT ID Valve Tune script needed ATTEMPTS e For valve serial number attempts switching to gt V 009800 replace position POSITION the valve 70 ERROR_PARALLEL_ Contact closure port Contact Thermo Fisher PORT_WRITE IO error Scientific 71 ERROR PARALLEL _ Contact closure port Contact Thermo Fisher PORT_NOT_ IO error Scientific INITIALIZED 72 ERROR PARALLEL _ Contact closure port Contact Thermo Fisher PORT_MASS_SPEC_ IO error Scientific BUSY 73 ERROR PARALLEL _ Contact closure port Contact Thermo Fisher PORT_MASS_SPEC_ IO error Scientific
154. contact closure Action If you are running two way contact closure with feedback from the mass spectrometer set the contact closure Protocol setting to Two way on the Configuration gt Connections page If the mass spectrometer is waiting for a signal set the contact closure State setting to Open If you are running one way contact closure that is the EASY nLC instrument sends a start signal to the mass spectrometer but feedback from the mass spectrometer is ignored set the contact closure Protocol setting to One way on the Configuration gt Connections page Table 11 contains troubleshooting tips for instrument startup problems Table 11 Startup troubleshooting tips Symptom Possible causes Dark monitor Broken or open main power fuse Action 1 Turn on the power to the instrument 2 Remove the right side panel of the EASY nLC instrument 3 If there is no light on the pumps turn off the EASY nLC instrument replace the right side panel and check the fuse Defective monitor Attach an external monitor to the VGA connector labeled MONITOR on the back panel of the instrument see Figure 16 on page 16 Startup bar is no longer Damaged instrument proceeding 172 EASY nLC Series Troubleshooting and Maintenance Guide Turn off the EASY nLC instrument wait 5 seconds before turning it on again and then wait approximately 10 minutes for the restart process to finish In some cases this recovers
155. cool Fire Use care when operating the system in the presence of flammable gases Eye Hazard Eye damage could occur from splattered chemicals or flying particles Wear safety glasses when handling chemicals or servicing the instrument General Hazard A hazard is present that is not included in the above categories Also this symbol appears on the instrument to refer the user to instructions in this manual When the safety of a procedure is questionable contact your local Technical Support organization for Thermo Fisher Scientific San Jose Products VORSICHT Elektroschock In diesem Ger t werden Hochspannungen verwendet die Verletzungen verursachen k nnen Vor Wartungsarbeiten mu das Ger t abgeschaltet und vom Netz getrennt werden Betreiben Sie das Ger t nicht mit abgenommenem Deckel Nehmen Sie die Schutzabdeckung von Leiterplatten nicht ab Chemikalien Dieses Ger t kann gef hrliche Chemikalien enthalten Tragen Sie Schutzhandschuhe beim Umgang mit toxischen karzinogenen mutagenen oder tzenden reizenden Chemikalien Entsorgen Sie verbrauchtes OI entsprechend den Vorschriften in den vorgeschriebenen Beh ltern Hitze Warten Sie erhitzte Komponenten erst nachdem diese sich abgek hlt haben Feuer Beachten Sie die einschl gigen Vorsichtsma nahmen wenn Sie das System in Gegenwart von entz ndbaren Gasen betreiben Verletzungsgefahr der Augen Verspritzte Chemikalien oder kleine Partikel k nne
156. create a new plate format 1 Open the Tools view for the autosampler as follows a Press Maintenance gt Devices b In the Devices list select the autosampler device c Press the Tools tab Figure 242 shows the Tools view for the autosampler Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 275 7 Calibrating the Autosampler s XYZ Robot Managing Plate Formats 2 Do one of the following e To create a new plate format go to step 3 P g P e To copy an existing plate format copies the format selection go to step 4 3 Create a new plate format as follows a e Press New The Create New Plate Format dialog box opens Figure 243 Figure 243 Create New Plate Format dialog box Create new plate format Format 16x24 HT MTP Name am a Accept Cancel In the Format list select a plate format 6 x 8 vials 8 x 12 96 well plates or 16 x 24 384 well plates In the Name box enter a name for the new plate format Press Accept The new plate name appears in the plate list with the text uncalibrated appended on the right Go to Calibrating Plates on page 280 to calibrate the vial or well positions 4 Copy an existing plate format as follows a Select an existing plate format from the Plate list b Press Copy To The Copy Plate Format dialog box opens In the Name box type a name for the new plate format Press
157. ct 2011 08 49 admin 200 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific Thermo Scientific 6 Troubleshooting Troubleshooting Tips An autosampler icon turning pink means that the communication to the unit has been lost Figure 206 shows the pink autosampler icon on the Home gt Overview page Figure 206 Pink autosampler icon on the Home gt Overview page up Method Editor Mainten ifiguration SCIENTIFIC LSP disabled in finnites Convelied Idle flow disabled O nl min 40 0 140 0 pl Pickup sam ple Initialize sys Refill AB Load sample Equilibrate p Prealign A B Equilibrate an Aut osampler wash refill S al column Cradient s olvent B Job 00 00 00 Eject plate Insert plate Current 1 Nov 2011 14 55 admin EASY nLC Series Troubleshooting and Maintenance Guide 201 6 Troubleshooting Troubleshooting Tips Miscellaneous For additional issues that might arise while using the EASY nLC instrument consult these miscellaneous troubleshooting tips Table 22 Table 22 Miscellaneous troubleshooting tips Sheet 1 of 2 Symptom Copying log files to a USB memory stick failed Possible causes Memory stick in wrong format Action Format the memory stick in FAT FAT16 format Memory stick not recognized by the instrument Use one of the memory sticks supplied by Thermo Fisher Scientific or try plugging the stick into the other USB port Contac
158. ct a Device to Add dialog box 5 Verify that the device was added to the system by locating it in the Devices list Select the device in the Devices list and review the information on the About page To remove a device from the Devices list 1 Press Maintenance gt Devices 2 Select the device in the Devices list 3 Press Remove Device The Remove Device dialog box appears 4 Press Accept to remove the device and return to the Maintenance gt Devices page Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 105 4 Routine Maintenance Managing the Devices List KD To view information about a device 1 Press Maintenance gt Devices 2 Select the device in the Devices list 3 Press the About tab The About view contains information about the serial numbers and firmware versions for the rotary valves piston syringe pumps autosampler and HPLC 106 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific ee i Field Service Maintenance This chapter provides procedures for use by Thermo Fisher Scientific field service engineers on replacing the subassemblies in the EASY nLC instrument Only Thermo Fisher Scientific field service engineers can perform these procedures CAUTION Only Thermo Fisher Scientific field service engineers can perform the procedures in this chapter Contents e Replacing the Built In Computer e Replacing the Monitor e Replacing a Pressure Sens
159. ct the flow sensors to the new flow sensor bracket supplied in the Pump Mounting Kit Note The mounting holes in the new flow sensor bracket are lower than the mounting holes in the original flow sensor bracket When you connect the new bracket to the instrument panel it is positioned higher than the original bracket allowing more space for the tubing that is connected to the pump head Figure 174 New flow sensor bracket with lower mounting holes Lower mounting holes in the new flow sensor bracket Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 161 5 Field Service Maintenance Replacing a Pump 4 Using a 2 5 mm hex wrench and the screws that you set aside when you removed the original flow sensor bracket from the instrument panel connect the new flow sensor bracket to the instrument panel Reconnect the tubing to the inlet and outlet ports of the flow sensors Be careful when installing the fused silica line to flow sensor B as the end of the tubing is fragile CAUTION Take care when you connect the fused silica line to the outlet port of flow sensor B The end of the tubing is fragile and easily damaged Using a 2 5 mm L hex wrench or ball driver and the four screws provided in the Pump Mounting Kit secure the pump adapter plate to the instrument panel Figure 175 Securing the pump adapter plate to the instrument panel E i Pump adapter plate Install the PLU pump in the
160. d The two temperature sensors measure this temperature asymmetry Because the temperature distribution depends on both the solvent flow rate and the solvent mixture you must recalibrate the flow sensors whenever you change the solvent types for solvents A and B For example if you use methanol instead of acetonitrile for the solvent B mixture run the script Calibrate Flow Sensors on page 51 The EASY nLC 1000 instrument uses two flow sensors with a maximum measuring range of 5 0 pL min The EASY nLC II instrument uses two different flow sensors The maximum measuring range is approximately 2 9 pL min for flow sensor A and 4 5 pL min for flow sensor B The wetted path for the EASY nLC 1000 instrument includes external inline filters for the flow sensor inlets and outlets When the inline filters become clogged they must be replaced For the EASY nLC II instrument the tubing that connects the valve to the flow sensor contains a built in filter Check Valve Assemblies Thermo Scientific When the corresponding high pressure 6 port rotary valves for pumps A or B are in position 1 2 the check valves perform these functions e The solvent side check valve prevents backflow into the associated solvent inlet line when the associated pump is emptying solvent to the waste beaker e The waste side check valve prevents backflow from the waste beaker when the associated pump is filling and drawing solvent from the associated solvent bo
161. d Maintenance Guide Thermo Scientific 6 Troubleshooting Troubleshooting the Results of the System Leak Test Figure 216 Workflow for locating leaks in the solvent path between valves S and W or in these valves Part 2 Continued from Figure 215 For the EASY nLC 1000 redo the nanoViper connection to port 2 of valve W step 4a on page 230 For the EASY nLC Il use a 1 4 in open ended wrench to tighten the stainless steel fitting in port 2 of valve W No Readback for flow sensor A gt 100 nL min Done Yes Disconnect the Waste In line from the leak test union step 5a on page 231 Then block the free end of the union with a blind fitting y No Readback for flow sensor A gt 100 nL min Install a new Waste In line Then rerun the system leak test page 218 Yes For the EASY nLC 1000 redo the nanoViper connection to port 3 of valve S step 6a on page 232 For the EASY nLC Il use a 1 4 in open ended wrench tighten the stainless steel fitting in port 3 of valve S y Readback for flow sensor A gt 100 nL min Disconnect the Column Out line from port 3 of valve S step 7a on page 233 Then block port 3 of valve S with a blind fitting Readback for flow sensor A gt 100 nL min Reconnect the solvent lines and rerun the No system leak test to confirm that the system is leak tight page 218 Install
162. d a failure during Prepare For Run command Thermo EASY nLC The hat been paused Ready to Download To resume the list go to the Actions main menu LTQ Velos MS and uncheck the Pause Analysis menu item Ready to Download Lx Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 191 6 Troubleshooting Troubleshooting Tips Figure 200 shows the Home Page error message that appears when the sequence list contains a well plate position and the autosampler configuration is set up for the vial adapter plate format Figure 200 Incorrect vial position for the vial adapter plate format Invalid vial position t4 Thermo Xcalibur Senuence 110414132511 Open hange aae Deele le m ao Typ File Name Sample ID Beam Status TE Unknown BlankUDGI A2 a Run Manager 2 Unknown BlankUDG2 A3 C XcaliburD C Xcalibur m Ready To Download 3 Unknown BlankUDG3 A4 C Xcalibur D C Xcalibur m Sequence 4 Unknown 45 C Xcalibur D C Xcalibur m Sample Name Working On Position Home Page Raw File Inst Method Invalid Autosampler Vial Position Thermo EASY nLC A valid example vial would be A1 Ready to Download LTQ Velos MS Ready to Download 192 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 6 Troubleshooting Troubleshooting Tips Figure 201 shows that the mass spectrometer is waiting for a start signal from the EASY nLC device before it starts data
163. damage could occur from splattered chemicals or flying particles Wear safety glasses when handling chemicals or servicing the instrument General Hazard A hazard is present that is not included in the above categories Also this symbol appears on the instrument to refer the user to instructions in this manual When the safety of a procedure is questionable contact your local Technical Support organization for Thermo Fisher Scientific San Jose Products EREE US OHWSIMRETERL Ak Att DTE ET RTF EER UTRREFEL MRED OTHORMLTFEY EMH AEA LIES THNAA 7Y hR RORMBA i ROTE LEDER ERAAN PELTOA TEED Y ET BEE EP ATE PAE ER MRE LOHDSRHERYVRIM FRE MALTFAY EMOANN MEODSBL FMEHRALTFS WR WAS ROKMMIMADOL HO TH ORF EBEAOTIS AI MED HADES SBM CL AF ALMETSBSIL KORE EMOTE RET BER CFR RLF ATK L TRE SoD OE T EFMRORMYORV HSVILHMBORT SICH L TSMR MALTESE RRIRER ORMISLIEUADS 4 TORRBAETS OL ERLE T Rk HRB COMMA TWSHEI Krz FLenn RLTFS RSMAS AFE bhb Ft BL QY EILY bOVHVO tIOFI OFI hbt ke Va cCCBBC EE ER EE G2 RERARARPERKRARHESHRRKRER EHEZH gt LAA RERA ERER o FLAERESLHHAP RH th 0 th 2 HF PCB REE LEG RERA TEREA AREE Eo EMH RH KERAN ADECER HRAFFRo KREG A BR HR Bi HT HE BEF o Hh AER ALKA EE o KR EA RAE REAR gt WB LD CBE RSE ER RRS RRA Ee Ro RBM AE ee EAE AR SS HK A OF BLM HK SMR HF RKERR HARARE rRe o ht RS Be ER AR UE RA PARAS AP YK o ORY de AEE ABE ie
164. ded wrench to tighten the fittings e For the nanoViper fittings in an EASY nLC 1000 instrument follow the instructions in Using nanoViper Fittings on page 83 to reconnect the nanoViper solvent lines to the valve ports Take care not to overtighten these fingertight fittings To remove air from the system and check for leak follow To remove air from the system and check for leaks after disassembling a valve on page 76 EASY nLC Series Troubleshooting and Maintenance Guide 79 4 Routine Maintenance Replacing the Check Valves Replacing the Check Valves If a check valve is not functioning replace it Replacing a check valve requires the following tools and materials Tools Parts and materials e 9 16 in open ended wrench e Powder free safety gloves e 2 Phillips head screwdriver e Solvent side check valve P N LC233 e Waste side check valve P N LC234 CAUTION Wear powder free gloves and safety glasses when handling parts of the LC system that come into contact with solvents To replace a check valve 1 Close down the EASY nLC system and then turn off the power to the instrument see Closing Down the EASY nLC Instrument on page 21 2 Using a 2 Phillips head screwdriver with a quarter turn loosen the three captive screws that secure the right side panel to the instrument housing Then remove the panel 3 Do the following e Fora solvent side check valve remove the solvent inlet line from the c
165. ding on whether the Flush Air script passed or failed do one of the following e Ifthe Flush Air script failed leave the Maintenance gt Scripts page open and go to step 4 to determine if the autosampler needle is blocked e Ifthe Flush Air script passed leave the Maintenance gt Scripts page open and go to step 5 to determine if the autosampler requires calibration 4 To determine if the autosampler needle is blocked do the following a Using a 1 4 in open ended wrench disconnect the PEEKsil autosampler needle from port 1 of valve S b Disconnect the Teflon waste line from port 3 of valve W and connect it to port 1 of valve S c Place the free end of the Teflon line in an HPLC vial filled with solvent A d To open the Purge script select Purge in the Name list e Select the Purge Pump S check box f Press Start g Verify that pump S aspirates solvent from the HPLC vial h Do one of the following e If pump S can aspirate solvent through the Teflon waste tubing the needle is blocked Reconnect the Teflon waste tubing to port 3 of valve W and replace the autosampler needle see Replacing the Autosampler Needle on page 84 e If pump S cannot aspirate solvent through the Teflon waste tubing go to Troubleshooting a Pump that Fails the Flush Air Script on page 212 5 To determine if the autosampler needle is going to the correct position and depth go to Checking Sample Pickup on page 263 262 E
166. do si usa il sistema in presenza di gas infiammabili Pericolo per la vista Gli schizzi di prodotti chimici o delle particelle presenti nell aria potrebbero causare danni alla vista Indossare occhiali protettivi quando si maneggiano prodotti chimici o si effettuano interventi di manutenzione sull apparecchio Pericolo generico Pericolo non compreso tra le precedenti categorie Questo simbolo utilizzato inoltre ull apparecchio per segnalare all utente di consultare le istruzioni descritte nel presente manuale n Quando e in dubbio la misura di sicurezza per una procedura prima di continuare si prega di mettersi in contatto con il Servizio di Assistenza Tecnica locale per i prodotti di Thermo Fisher Scientific San Jose CAUTION Symbol PPP PRP o CAUTION Electric Shock This instrument uses high voltages that can cause personal injury Before servicing shut down the instrument and disconnect the instrument from line power Keep the top cover on while operating the instrument Do not remove protective covers from PCBs Chemical This instrument might contain hazardous chemicals Wear gloves when handling toxic carcinogenic mutagenic or corrosive or irritant chemicals Use approved containers and proper procedures to dispose waste oil Heat Before servicing the instrument allow any heated components to cool Fire Use care when operating the system in the presence of flammable gases Eye Hazard Eye
167. e Flow Sensors The Flow Sensors script calibrates the flow sensors for pumps A and B Use this script to do the following e Periodically check the accuracy of the flow sensor calibration for your maintenance records select the Inspection Only check box e Calibrate the flow sensors when you change the type of solvent in solvent bottles A or B clear the Inspection Only check box This calibration does not require any additional plumbing IMPORTANT Because the flow sensor calibration requires a stable operating temperature ensure that the instrument s side panels are installed and that the instrument has been on for a minimum of one half hour Figure 40 shows the parameters for the Flow Sensors script Figure 40 Flow Sensors script parameters Thermo Home Batch Setup Hede azio Maintenance Eesaia SCIENTIFIC fc libi fii No graph X Log Book alibrate Flow sensors v Support pE Parameters Mera Devices Parameter Include sensor A Include sensor B Inspection only Exact inspection f Fast inspection No graph X Job 00 15 48 11 May 2011 12 54 admin Schedule Exit Torun the Flow Sensors script 1 Open the Flow Sensors script as follows a Press Maintenance gt Scripts b In the Category list select Calibrate c Inthe Name list select Flow Sensors Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 51 3
168. e matches the installed sample loop Lowercase letter used in the sequence table for vial position See Figure 199 on page 191 Change the letters to capitals Unable to submit samples The following error message appears Invalid Autosampler Vial Position Thermo Scientific Incorrect plate configuration See Figure 200 on page 192 EASY nLC Series Troubleshooting and Maintenance Guide Select the correct autosampler plate in the Thermo EASY nLC Configuration dialog box 189 6 Troubleshooting Troubleshooting Tips Table 17 Possible causes of errors reported by the Xcalibur data system Sheet 2 of 2 Symptom Possible cause Action The mass spectrometer remains in Contact closure configured Press Configuration gt Connections the Waiting for Contact Closure incorrectly state and data acquisition does not Select the correct MS configuration and start see Figure 201 on page 193 settings Failing contact closure relay Check accordingly to Verifying that the due to incorrect grounding LC MS System Is Properly Grounded on page 268 Figure 197 shows the Acquisition Server message that appears when the EASY nLC instrument loses communication with the data system computer during a sequence run The sequence pauses until you intervene Figure 197 Lost connection between the EASY nLC instrument and the Acquisition Server u velos 10 32 134 20 Remote Desktop DEAR Thermo Xcalibur Sequence Setup
169. e 229 shows the areas that the following procedure systematically checks for blockage Figure 229 Solvent path from pressure sensor A to the Waste In line Valve W 2 6 1 Valve W 2 Waste In line 345 Note Disconnect the Column Out and the Waste In lines from the union after testing valve W for blockage Valve S 3 Column Out line 4 Valve S ale f 345 5 Valve S port 4 to mixing Tee 6 Mixing Tee 7 Mixing Tee to flow sensor A 13 Valve A port 1 to outlet pressure sensor 8 Inline filter Valve A EASY nLC 1000 instrument X 9 Flow sensor A m 10 Inline filter 2 1 6 EASY nLC 1000 instrument 345 12 Valve A 11 Valve A port 6 to flow sensor inlet 252 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 6 Troubleshooting Troubleshooting a System Blockage To locate the source of the blockage when the Test Solvent A test fails 1 To determine if valve W is blocked do the following a Make sure that the Precolumn Equilibration script is set up and running as described in step 1 on page 251 and that the Column Out and Waste In lines are connected with a union b Disconnect the Waste In line from port 2 of valve W see Figure 229 on page 252 c Depending on the back pressure do one of the following e Ifthe pressure is below the threshold valve W is blocked Go to Maintaining the Rotary Valves on page 74
170. e ASA model go to step 5 e For the ASC model go to step 6 5 For the ASA model move the z axis needle holder to position A1 as follows a Press Home gt Overview b Press the Autosampler icon The Autosampler dialog box opens c Inthe XYZ Robot area select position Al in the Well box see Figure 75 Figure 75 Autosampler direct control dialog box XYZ Robot C Eject Plate Insert Plate 6x8 standard vials Goto Calibration Goto Well f f Needle Up Needle Down f f 7 0 Set Disable Temperature 2G Close d Press Goto e Go to step 7 on page 86 6 For the ASC model move the z axis needle holder to position W1 as follows a Press Home gt Overview b Press the Autosampler icon The Autosampler dialog box opens Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 85 4 Routine Maintenance Replacing the Autosampler Needle c Inthe XYZ Robot area press Go to Calibration The Tools page of the Autosampler view on the Maintenance gt Devices page opens see Figure 76 Figure 76 Tools page of the Autosampler view on the Maintenance gt Devices page SCIENTIFIC Devices Remove Device EASY nLC HPLC HPLC Piston Pump flow feedback Pump A Piston Pump wi flow fee Add Device e Cooled Autosampler ASC Autosampler Plate Vials Wash Calibrate Current 8x12 4bgene 4B 0800 Calibrate Job 00 00 00 Mani
171. e Ifthe pressure is below the threshold valve S is partially or totally blocked Discontinue this troubleshooting procedure and go to Maintaining the Rotary Valves on page 74 Instrument Pressure threshold EASY nLC II 100 bar EASY nLC 1000 90 bar Ifthe pressure is above the threshold reconnect the mixing Tee line to port 4 of valve S and then go to step 5 5 To determine if the line that connects valve S to the mixing Tee is blocked do the following a Disconnect the line from the mixing Tee b Depending on the back pressure do one of the following Ifthe pressure is below the threshold the line that connects the mixing Tee to valve S is blocked Replace it Instrument Pressure threshold EASY nLC IT 100 bar EASY nLC 1000 65 bar e Ifthe pressure is above the threshold reconnect the line to the mixing Tee and then go to step 6 6 To determine if the mixing Tee is blocked do the following a Disconnect the line that exits flow sensor A from the mixing Tee b Depending on the back pressure do one of the following e Ifthe pressure is below the threshold the mixing Tee is blocked Clean the mixing Tee or if necessary replace it Instrument Pressure threshold EASY nLC II 100 bar EASY nLC 1000 65 bar e Ifthe pressure is above the threshold reconnect the line to the mixing Tee and then go to step 7 254 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 10 Thermo
172. e Thermo Scientific 1 Introduction Hardware Components Table 1 Valve functions Component Valves A and B Function Direct the solvent flow from syringe pump A and B respectively 1 2 position Directs the solvent flow toward the check valve When the valve is in the 1 2 position the pump draws solvent from the solvent bottle on top of the instrument or empties solvent to the waste beaker 1 6 position Directs the solvent flow toward the mixing Tee Centered e Prevents backflow into the solvent line upstream of the mixing Tee e Allows the back pressure for the solvent A and B lines to equalize before the gradient step of a sample run e Blocks the solvent path through the valve and to the subsystem for several maintenance scripts Valve S Directs the solvent flow from syringe pump S and the solvent flow from the mixing Tee 1 2 position The solvent flow bypasses the sample loop 1 6 position The solvent flow passes through the sample loop Centered Isolates the pump S subsystem for the Flush Air script e Blocks the solvent path after valve S for the system leak test Leaks script with the System check box selected e Shuts off the flow to the Column Out line and provides back pressure during the Flow Sensor Calibration script Valve W Thermo Scientific Directs the solvent flow through the venting Tee to the waste beaker or provides pressure venting to the system 1 2 p
173. e check valve assembly is functioning properly go to step 4 212 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 6 Troubleshooting Troubleshooting a Pump that Fails the Flush Air Script 4 Check whether the pump can draw solvent through the tubing that connects the pressure sensor to the rotary valve as follows a Using a 1 4 in open ended wrench disconnect the solvent line from the rotary valve see Figure 211 e For pumps A or B disconnect the solvent line from port 1 of the valve e For pump S disconnect the solvent line from port 6 of the valve Figure 211 Rotary valve layout for the EASY nLC 1000 instrument Port 1 of valve A Port 6 of valve S Port 1 of valve B Valve S Valve W fe ne nw nw b Place the free end of the solvent line into a small vial that contains the appropriate solvent Use solvent A for pump A and solvent B for pump B c Run the Purge Solvent script and check that solvent is being aspirated from the vial into the pump For information about the Purge Solvent script see Prepare Purge Solvent on page 32 d Depending on whether the pump aspirated the solvent do one of the following e Ifthe pump does not aspirate solvent from the vial go to step 5 e Ifthe pump aspirates solvent from the vial the problem is probably resolved To verify that
174. e fitting and remove or pull up the autosampler needle from the needle holder Figure 114 shows the autosampler needle connected to the needle holder Figure 114 Needle connected to the needle holder Autosampler needle fitting ony A 1 5 Unplug the bus cables on the left side of the instrument from the autosampler cooler and horizontal XYZ axis PCB see Figure 115 Figure 115 Bus cables a z 7 Cooler bus cable XYZ axis PCB bus cable Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 123 5 Field Service Maintenance Replacing the ASC Autosampler 6 Cut the tie wrap attaching the cables to the vertical part of the autosampler frame as shown in Figure 116 Figure 116 Cutting the strip attaching the cables to the autosampler frame m a W 7 Using a Torx T 10 screwdriver remove the screws that secure the back horizontal cable bar to the autosampler frame see Figure 117 Figure 117 Horizontal cable bar after removal of screws gori Bus cable connected to valve B Back left screw that secures the Autosampler autosampler to the instrument frame Horizontal cable bar 124 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 5 Field Service Maintenance Replacing the ASC Autosampler 8 Move the cable bar to the side this is easier to do if you also remove the bus cable to valve B See Figure 118 Figure 118 Horizontal cable bar moved aside Valve B
175. e flow during the column equilibration and sample loading steps If the gradient is short a leak upstream of flow sensor B will cause a complete lack of signal because solvent B is not reaching the columns within the duration of the gradient Figure 192 Typical pump B profile for a leak upstream of flow sensor B Thermo Home Batch Setup Method Editor Maintenance Configuration SCIENTIFIC Overview Pump B Craps Desired flow Real flow Pressure Flow ni min eq aunssasg Job 00 28 18 Sample 00 28 18 Gradiem 20 00 18 jul 2011 12 56 admin 125 15 0 Exit Time min Negative flow during the column equilibration and sample loading steps 186 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 6 Troubleshooting Troubleshooting Tips Slow or No Pressure Increase in Subsystem A or B A lack of pressure or a slow increase in pressure in subsystem A or B is often caused by a worn piston seal a worn rotor seal or a leaking tubing connection Note A subsystem includes the pump the solvent line that connects the pump to the pressure transducer the solvent line that connects the pressure transducer to the valve and the valve Table 16 Troubleshooting low back pressure in subsystems A or B Symptom Back pressure is low in subsystem A or B Thermo Scientific Possible cause Action Air in the pump Run the Purge Solvent script for the affected pump with 2 itera
176. e m 3 Maintenance Scripts and Service Records Maintenance Scripts Test Sample Pickup The Sample Pickup script tests the accuracy of the autosampler pickup function During the script the XYZ robotic arm moves to the specified sample location Then pump S aspirates the specified volume from the vial or microplate well and ejects this volume to waste To determine if the autosampler is aspirating the specified sample volume weigh the sample both before and after you run the test script as described in Checking Sample Pickup on page 263 Use this script to verify the calibration of the XYZ robotic arm and to determine the appropriate flow rate aspiration rate by pump S setting in the method or Xcalibur instrument method that you plan to use for your samples The appropriate flow rate depends on the sample viscosity Figure 33 shows the parameters for the Sample Pickup script and the Position dialog box that opens when you press the value box for the Position parameter Figure 33 Sample Pickup script parameters Thermo Home Batch Setup Method Editor SCIENTIFIC Log Book g X Fn pickup 4 Support Dmi Parameters Output Devices Parameter Volume pl Select position Flow l min i v 0 Plate vials A Position Job 00 00 00 Accept Cancel 6 Sep 2011 11 26 Exit i 42 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 3 Main
177. e of the following e Ifthe pressure is lt 20 bar flow sensor B is blocked Replace it as described in Replacing a Flow Sensor on page 92 e Ifthe pressure is gt 20 bar keep pump B running and go to step 5 for the EASY nLC 1000 instrument or step 6 for the EASY nLC II instrument For the EASY nLC 1000 instrument to determine if the inline filter connected to the inlet of flow sensor B is blocked do the following a Remove the inline filter from the flow sensor outlet b Depending on the back pressure do one of the following e Ifthe pressure is lt 20 bar the inline filter is blocked Replace it with a new filter e Ifthe pressure is gt 20 bar reconnect the inline filter to flow sensor A and then go to step 6 To determine if the line that connects valve B to flow sensor B is blocked do the following a Disconnect the line from port 6 of valve B and then monitor the pressure b Depending on the pressure do one of the following e Ifthe pressure is below the threshold the solvent line is blocked Discontinue this troubleshooting procedure and replace the solvent line that connects port 6 of valve B to the flow sensor inlet Instrument Pressure threshold EASY nLC II 20 bar EASY nLC 1000 10 bar e Ifthe pressure is above this threshold keep the pump running and go to step 7 EASY nLC Series Troubleshooting and Maintenance Guide 259 6 Troubleshooting Troubleshooting a System Blockage 7 To dete
178. e pressure sensor cable from the pump Figure 82 shows the pressure sensor connections Figure 82 Pressure sensor connections One of two screws that secure the pressure sensor to the L bracket Screws that secure the L bracket to the instrument panel Solvent line connections One of two screws that secure the pressure sensor to the L bracket Pressure sensor cable Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 91 4 Routine Maintenance Replacing a Flow Sensor To install the new pressure sensor 1 Using a 2 mm L hex wrench and the screws that you removed in step 6 on page 91 secure the new pressure sensor to the bracket 2 Using a 2 5 mm L hex wrench or ball driver and the screws that you removed in step 5 on page 91 reconnect the pressure sensor bracket to the interior right panel of the instrument 3 Reconnect the stainless steel solvent lines to the inlet and outlet ports of the pressure sensor Using a 1 4 in open ended wrench tighten the fittings 4 Mount the right side panel to the instrument housing Then with a 2 Phillips head screwdriver secure the panel by rotating the three captive screws a quarter turn clockwise 5 Reconnect the pressure sensor cable 6 Run the Flush Air script as described in Prepare Flush Air on page 33 Replacing a Flow Sensor The procedure for replacing the flow sensor differs bet
179. e system follow the solvent system schematics in Appendix C Consumables and Replacement Parts Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 147 5 Field Service Maintenance Replacing a Rotary Valve 5 Using a 2 5 mm L hex wrench remove the four 2 5 mm hex screws that keep the valve in place inside this compartment As you remove the last hex screw the valve might slip down into the autosampler compartment 6 From the back of the instrument disconnect the cables shown in Figure 159 from the valve to be exchanged Figure 159 Cables connected to valves 7 Pull out the valve as shown in Figure 160 Figure 160 Pulling out a valve Note If there is a support collar between the body of the valve and the body of the EASY nLC instrument move it to the new valve 148 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific Thermo Scientific 5 Field Service Maintenance Replacing a Rotary Valve To install a new rotary valve Using a small flathead screwdriver set the rotary switch on the new valve of the PCB shown in Figure 161 to the same address as the valve being replaced e Valve A 1 e Valve B 2 e Valve S 3 e Valve W 4 Figure 161 Valve rotary switch Rotary switch Insert the new valve making sure that the valve PCB faces down In the back of the instrument connect the cables shown in Figure 159 on page 148 to the new valve Usin
180. e the right side panel to the instrument housing Then remove the panel Using a 9 64 in L hex wrench remove the two hex screws that secure the stator to the valve assembly and then pull the stator away from the valve driver Depending on whether you are cleaning or replacing the rotor seal go to the appropriate procedure e To clean the rotor seal and the stator on this page e To replace the rotor seal on page 77 Note If you are cleaning the valve components or replacing only the rotor seal you do not need to remove the tubing connected to the stator If you are replacing the stator you must remove all the solvent lines from the valve To clean the rotor seal and the stator If you have not already done so remove the stator from the valve as described in the previous procedure To remove the stator from the valve Leave the rotor seal mounted to the valve driver see Figure 66 Clean the mounted rotor seal with a lint free tissue or Q tip soaked in methanol Figure 66 Rotor seal mounted on the valve driver Rotor seal Clean the stator with a lint free tissue or Q tip soaked in methanol see Figure 67 Figure 67 Cleaning the stator EASY nLC Series Troubleshooting and Maintenance Guide 75 4 Routine Maintenance Maintaining the Rotary Valves 76 To reassemble the cleaned valve and return the instrument to normal operation Mount the clean stator onto the valve driver
181. e z axis needle holder avoid placing tall objects into the tray compartment The autosampler needle draws solvent from wash bottle W3 to perform a standard wash and ejects the solvent into wash bottle W4 which contains the needle wash insert The autosampler can also perform a custom wash using the solvents from wash bottles W1 W3 The waste beaker to the right of the tray compartment collects solvent that is delivered by pumps A and B when these pumps empty solvent to waste This waste beaker also collects solvent delivered by pump A during the precolumn equilibration and sample loading steps for a two column setup The EASY nLC Series includes two supported versions of the autosampler module e ASC Model Current on page 3 e ASA Model Legacy EASY nLC II Instruments on page 4 Note The ASC model has an integrated cooling compartment which ensures stable temperature conditions throughout the entire vial plate By contrast the ASA model is cooled from underneath only 2 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 1 Introduction Hardware Components The following differences between the two autosampler models affect the routine calibration and maintenance procedures e The z axis needle holders and the autosampler needles This difference affects the autosampler needle replacement procedure e The location of the wash bottles This difference affects the autosampler calibration pr
182. eak and completed this troubleshooting procedure Return the instrument to normal operation e Ifthe flow rate is gt 100 nL min go to the next step To check the solvent line that connects the mixing Tee outlet to port 4 of valve S do the following a On the Home gt Overview page set valves A and W to the following positions to return the system to atmospheric pressure e Valve A Center e Valve W 1 6 For information about changing the valve positions see Using the Valve Controls on page 204 Disconnect the nanoViper fitting from the mixing Tee outlet Then block the mixing Tee outlet with a blind fitting see Figure 222 236 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 6 Troubleshooting Troubleshooting the Results of the System Leak Test Figure 222 Mixing Tee outlet plugged with a blind fitting Waste In line Leak test union Valve A Valve B Column Out line Solvent line that connects the mixing Tee outlet to port 4 of valve S Inline filter connected to the outlet of lt Blind fitting connected to the mixing Tee outlet flow sensor A ee ee Inline filter connected to the outlet of flow sensor B c On the Home gt Overview page reset these valve positions e Valve A 1 6 e Valve W 1 2 Thermo Scientific EASY nLC Series Troubleshooting a
183. ean Safety and EMC Electromagnetic Compliance Standards Application of Council Directive s 2006 95 EEC Low Voltage Intertek Group plc 89 336 EEC Electromagnetic Compatibility DELTA Denmark Standard s to which conformity is declared Manufacturers Name Manufacturers Address EN61010 1 2001 Second edition Safety Requirements for Electrical Equipment for Measurement Control and Laboratory Use EN61010 2 81 Part 2 081 Particular requirements for automatic and semi automatic _ aboratory equipment for analysis and other purposes EN IEC 61326 1 2006 A1 1998 A2 2001 and A3 2003 EMC requirements for electrical equipment for measurement control and laboratory use Proxeon Biosystems A S Edisonsvej 4 DK 5000 Odense Denmark Type of Equipment Laboratory Instrumentation Model Name EASY nLC II Model Numbers LC110 LC111 Serial Number LC 000100 and later Year of Manufacture 2010 I the undersigned hereby declare that the equipment specified above conforms to the above Directive s and Standard s 7 y fe ALO Ole Vorm Site Manager August 31 2010 Declaration of Conformity for the EASY nLC 1000 System U S Safety and EMC Electromagnetic Compliance Standards Safety This instrument has been reviewed for compliance with standard UL 61010 1 Electrical Equipment for Measurement Control and Laboratory Use Part 1 General Req
184. ement Parts for the EASY nLC II Instrument e Common Replacement Parts for the EASY nLC 1000 Instrument Common Replacement Parts for the EASY nLC II Instrument Table 34 lists the annual consumption of common replacements parts for the EASY nLC II instrument Table 34 Common replacement parts for the EASY nLC II instrument Description Part number Quantity Pump Piston Seal LC210 4 piston seals 1 per pump Replacement Kit contains 1 extra four piston seals and the piston seal tool Valve rotor seal for valve LC224 8 rotor seals 2 per valve serial numbers V 009999 and below contains one rotor seal Valve rotor seal for valve LC228 8 rotor seals 2 per valve serial numbers V 010000 and above contains one rotor seal Column Out solvent line LC260 2 Waste In solvent line LC262 1 Autosampler needle ASA LC251 1 model Autosampler needle ASC LC302 2 model Valve to flow sensor line LC222 2 contains two flow sensor lines Replacement filter discs LC232 2 filter discs 1 per solvent contains two 10 pm filter bottle discs 318 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific C Consumables and Replacement Parts Common Replacement Parts Common Replacement Parts for the EASY nLC 1000 Instrument Thermo Scientific Table 35 lists the annual consumption of common replacements parts for the EASY nLC 1000 instrument Table 35 Common replacement parts for the EASY n
185. ens c Locate the autosampler device and select it d Press Accept to add the autosampler device to the Devices list and close the Select a Device to Add dialog box e Verify that the autosampler device was added to the system by locating it in the Devices list 10 Calibrate the autosampler as described in Chapter 7 Calibrating the Autosampler s XYZ Robot 104 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 4 Routine Maintenance Managing the Devices List Managing the Devices List The Device list on the Maintenance gt Devices page lists the current devices installed in the EASY nLC instrument When you add or remove a device that is not automatically detected during the boot procedure you must add the device to or remove the device from the Devices list on the Maintenance gt Devices page Devices that are not recognized during the boot procedure include the autosampler and the external RePlay device To add a device to the Device list 1 Press Maintenance gt Devices 2 Press Add Device The Select a Device to Add dialog box opens Advion RePlay Fluid Storage Autosampler ASA Autosampler Autosampler ASC Autosampler EASY nLC HPLC New Era NE 1000 Syringe Drive Proxeon EASY SPOTTER Liquid Depositor Accept Cancel 3 Locate the device in the list and select it 4 Press Accept to add the autosampler device to the Devices list and close the Sele
186. ensuring that the rotor sealing surface engraved flow passage is facing out 7 Mount the stator onto the valve driver 8 Using a 9 64 in L hex wrench tighten the two hex screws a little at a time by shifting from one screw to the other and back again until the screws are evenly torqued 9 Reconnect the right side panel 10 Turn on the EASY nLC instrument and log in as an administrator 11 Reset the usage counter for the valve as follows a Press Maintenance gt Devices b Select the valve from the list of devices c Press the Summary tab d Press Reset The value in the Rotor Shifts box resets to 0 12 If you replaced the rotor seal in Valve A or B flush air from the system as follows a On the Maintenance gt Scripts page select Prepare in the Category list Then select Flush Air in the Name list b Select the pump that is connected to the affected valve c Set the flush volume threshold to 10 pL for the EASY nLC II or 12 pL for the EASY nLC 1000 d Press Start e Wait for the script to finish 13 Run the Leaks script as follows a Select Test in the Category list Then select Leaks in the Name list b Do one of the following e Ifyou replaced the rotor seal in Valve A or B run the Leaks script for the affected valve Ifyou replaced the rotor seal in Valve S or W run the Leaks script for the system When the system is leak tight the instrument is ready for use Replacing the St
187. entific 6 Troubleshooting Troubleshooting the Results of the System Leak Test Figure 223 Mixing Tee inlet for the solvent B channel plugged with a blind fitting Leak test union Valve A Valve B Solvent line that connects the mixing Tee outlet to port 4 of valve S Mixing Tee inlet for the solvent B channel plugged with a blind fitting Ba e On the Home gt Overview page check the readback for flow sensor A Then depending on the flow rate do one of the following e Ifthe flow rate is lt 100 nL min go to EASY nLC 1000 Locating a Leak Between Flow Sensor B and the Mixing Tee on page 243 as removing the solvent B channel from the flow path remedied the leak e Ifthe flow rate is gt 100 nL min the leak is in the solvent A channel as removing the solvent B channel from the solvent path did not remedy the leak Go to the next procedure EASY nLC 1000 Locating a Leak Between Flow Sensor A and the Mixing Tee Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 239 6 Troubleshooting Troubleshooting the Results of the System Leak Test EASY nLC 1000 Locating a Leak Between Flow Sensor A and the Mixing Tee By following the workflow in Figure 221 on page 235 or the procedure on pages 236 239 you determined that the leaking connection is between flow sensor A and the mixing Tee The
188. ents see Test Back Pressure on page 48 IMPORTANT The script evaluates the back pressure based on using 99 9 water for solvent A and 99 9 acetonitrile for solvent B To check the cooler temperature Check that the actual plate temperature matches its set point readout on the Home gt Overview page Figure 23 Cooler area of Autosampler dialog box Cooler ra A 7 N 70 Set J Disable J Temperature A Set point Actual temperature temperature To check the autosampler pickup function Run the Sample Pickup script to check the sample pickup function see Test Sample Pickup on page 42 To check the EASY nLC solvent system for leaks Run the Leaks script for A B and for System to check for and diagnose instrument leaks For more information see Test Leaks on page 44 and Running a System Leak Test on page 218 IMPORTANT Check the valve rotor shifts regularly When the valve rotor shifts exceed 6000 Thermo Fisher Scientific recommends checking the system for leaks From this point forward perform leak tests every 1000 shifts and replace the rotor seal when the leak test fails 28 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 2 Maintenance Schedule Yearly Maintenance To check the check valves Run the Purge Solvent script see Prepare Purge Solvent on page 32 and do the following e When the pu
189. eplace the rotor seal in valve S see Replacing the Rotor Seal on page 77 3 To return the EASY nLC instrument to normal operation as part of an LC MS system secure the right side panel reconnect the Column Out line to the column assembly and then reconnect the Waste In line to the venting Tee Checking the Sample Loop Connections for a Leak The system leak test returned the following message see Table 24 Check the sample loop for leaks The sample loop connections are leaking or the rotor seal in valve S is worn To fix the leaking sample loop connections or the leaking valve 1 Depending on the instrument model do one of the following e Foran EASY nLC II instrument use a 1 4 in open ended wrench to tighten the sample loop fittings connected to ports 2 and 5 on valve S e Foran EASY nLC 1000 instrument return the instrument to atmospheric pressure as described in the Using nanoViper Fittings Quick Reference Guide on page 323 Then individually disconnect and reconnect the nanoViper fittings connected to ports 2 and 5 on valve S Take care not to overtighten these fingertight fittings 2 Rerun the system leak test see Running a System Leak Test on page 218 3 If the script still fails clean the rotor seal and stator using methanol and a lint free swab Then rerun the system leak test as described in Cleaning the Rotor Seal and Stator on page 74 4 If the problem persists replace the rot
190. er cable to the back panel and to a laboratory power outlet e Ifthe instrument was connected to a laboratory LAN port reconnect the Ethernet cable e Ifthe instrument is part of a Thermo Scientific LC MS system reconnect the Ethernet cable to the back panel of the instrument and make sure that the other end of the cable connects to the Ethernet switch for the data system hardware 6 Turn on the EASY nLC instrument and log in as an administrator 7 Add the autosampler to the Devices list see Managing the Devices List on page 105 8 Recalibrate the autosampler see Chapter 7 112 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 5 Field Service Maintenance Replacing the Monitor Replacing the Monitor The procedure for replacing the monitor is the same for the EASY nLC 1000 instrument and the EASY nLC II instrument except where noted Replacing the monitor requires the following part and tools Tools Part e Torx T 10 wrench Monitor and adapter cable for computer EE E T E wnench boxes with a PS 2 keyboard connector as shown in Figure 16 on page 16 P N LC282 To replace the monitor follow these steps 1 To remove the monitor on this page 2 To install the new monitor on page 116 To remove the monitor 1 Close down the EASY nLC instrument see Closing Down the EASY nLC Instrument on page 21 and unplug the power cable 2 Depending on the instrument m
191. er occurs first Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 207 6 Troubleshooting Using the Direct Controls for Troubleshooting and Maintenance To empty pump A or B to waste 1 Check the position of the pump valve on the Home gt Overview page 2 Do one of the following e To empty pump A or B directly to waste make sure that its valve is in position 1 2 e To empty pump A or B to waste by way of valve W place the pump valve in position 1 6 and valve W in position 1 6 Valve Position Solvent flow AorB 1 2 Empties to the waste beaker 1 6 Passes through valve S and into the Column Out line S 1 2 Bypasses the sample loop 1 6 Passes through the sample loop W 1 2 Passes through the installed columns 1 6 Exits the Column Out line to waste for a one column setup or passes through the precolumn and then exits the system to waste for a two column setup 3 Press the pump icon for the pump you want to control The Pump dialog box opens see Figure 208 on page 206 4 In the flow rate box enter the flow rate 0 01 to 300 pL min When valve A or B is in position 1 6 the maximum system pressure limit constrains the maximum flow rate 5 In the volume box enter the volume that you want the pump to empty to waste 6 Press Start The pump empties until it pumps the requested volume or reaches the empty position whichever occurs first 208 EASY nLC Series Troubleshooting and Mai
192. eshooting and Maintenance Guide Thermo Scientific 5 Field Service Maintenance Replacing the Autosampler Cooler Replacing the Autosampler Cooler The procedure for replacing the autosampler cooler is the same for the EASY nLC 1000 and EASY nLC II instruments but it differs between an ASC cooler and an ASA cooler To replace the autosampler cooler follow the appropriate procedure e Replacing the ASC Cooler on this page e Replacing the ASA Cooler on page 140 Replacing the ASC Cooler Replacing the ASC cooler requires these items Tools Parts e Torx T 10 wrench ASC autosampler cooling module P N LC310 e 2 5 mm L hex wrench To replace the ASC cooler follow these steps 1 To remove the ASC cooler on this page 2 To install the new ASC cooler on page 139 To remove the ASC cooler 1 Close down and turn off the EASY nLC instrument see Closing Down the EASY nLC Instrument on page 21 Then disconnect the power cable 2 Using a Torx T 10 wrench remove the screws on the back cover panel and remove the panel 3 Access the PCB on the back of the cooler through the back of the instrument and unplug the P Bus communication cables shown in Figure 139 Figure 139 P Bus communication cables P Bus communication cables Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 137 5 Field Service Maintenance Replacing the Autosampler Cooler 4 Using a 2 5 mm ball drive
193. ess Login in the lower left corner of the touch screen see Figure 19 on page 19 The Login As User dialog box opens see Figure 20 Figure 20 Login As User dialog box a gt admin Password f gt r gt Accept Cancel 2 In the User list select a user with administrative rights 3 In the Password box enter the password for this user Tip The factory user name and password for the system administrator is admin 20 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 1 Introduction Touch Screen Application 4 Press Accept Figure 21 EASY nLC login dialog box r gt admin Password gt gt Accept Cancel Closing Down the EASY nLC Instrument Closing down the instrument in a controlled manner is important to allow all of the components to shut down in an orderly sequence Using this controlled method saves important data so that the instrument starts with the correct information the next time you use it IMPORTANT Ifyou turn off the power switch during normal operation you risk damaging essential system components To close down the EASY nLC instrument and turn off the power 1 Press Exit lower left corner of the screen A confirmation dialog box opens see Figure 22 Figure 22 Confirmation dialog box that opens after pressing Exit Administrator Logout Change password eZ l a top App
194. evrene ots deneri vori eiie Pade hemi 163 Replacing a Pump PCB in the PLU Pump 04 163 Replacing a Pump PCB in the PLF Pumps i4 is4viw pa tesa a ewes 167 Chapter6 Troubleshooting 0c ccc cece e eee eee eee eee eee 169 Tro bleshooting Tips respinse p ieena eP e tee teaweee Weald 170 Autosampler Problems nnn nuueeeeuunna narren nnee 171 Contact Closure Problems n n dhe wes Saeed ee Ae eek oe ees 172 Instrument Startup Problamiivss 6 wei kool ie nie ee mice Oops 172 Delayed El tions ass Ks ne aeiae haniai aae nng a 173 Excessive Duration or Higher Pressure for the Column Equilibration and Sample Loading Steps o oo u cob eed gles aaa Oks Es Be 178 System Reaches Its Maximum Pressure During the Gradient 180 Sample Signal Weak or Abs ttt lt scaisscacs sod ears kare he ae eae ee 183 Slow or No Pressure Increase in Subsystem A or B 00 000 eee 187 Errors Reported by the Xcalibur Data System 000000000 189 Garry OVEL srry tared as dtahiteerand catueleieybea tat beetyies san E 194 Spray ASSES ce wor a le Gets let Kilt Meads Pal a a Aaa ote tee des ae aly dana el 196 Chromatographic Performance seiicces ac dates aloes a activa o cde ae a whe 197 Device Failures sepiga fk scien on tenes Hoe led PARE Eb a AS 198 Miscellaneous spose ilcns aa eran here pitas Ehret deg 202 Using the Direct Controls for Troubleshooting and Maintenance 204 Using the Valve Co
195. ews 6 hex screws 272 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 7 Calibrating the Autosampler s XYZ Robot Replacing the Autosampler Adapter Plate 3 Place the new adapter plate on the tray see Figure 241 Figure 241 Tray for the ASA model autosampler 4 Fasten the screws in the following balanced order a Screw in the upper right corner b Screw in the lower left corner c Screw in the upper left corner d Screw in the lower right corner e Screws in the middle Note Mounting the screws in a balanced order can avoid distortion of the adapter plate The autosampler is now ready for new plate formats For information about selecting a plate format and creating a new plate format see Managing Plate Formats on page 274 After you create a new plate format perform the calibration routines described in this chapter Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 273 7 Calibrating the Autosampler s XYZ Robot Managing Plate Formats Managing Plate Formats When the EASY nLC system is initially installed the application includes plate formats for vials and microtiter plates Follow the appropriate procedure to use an existing plate format create your own plate format or delete a plate format that you no longer need e Selecting a Plate Format on this page e Creating a New Plate Format on page 275 e Deleting a Plate Format on page
196. g Troubleshooting the Results of the System Leak Test EASY nLC II Locating a Leak Between the Flow Sensors and Valve S Figure 228 on page 247 shows the solvent lines that connect the flow sensor outlets to the mixing Tee and the solvent line that connects the mixing Tee outlet to port 4 of valve S To systematically locate and remedy a leak in an EASY nLC II instrument from the flow sensor outlets to port 4 of valve S 1 To ensure a leak tight connection to port 4 of valve S do the following a Tighten the fitting in port 4 of valve S b From the Maintenance gt Scripts page start the Analytical Column Equilibration script with the maximum pressure set to 280 bar and the volume set to 140 pL For information about setting up the Analytical Column Equilibration script see step 1b on page 228 c On the Home gt Overview page check the readback for flow sensor A Then depending on the flow rate do one of the following e If the flow rate is lt 100 nL min you have remedied the leak and completed this troubleshooting procedure Return the instrument to normal operation e If the flow rate is gt 100 nL min go to the next step 2 Hand tighten the following PEEK fittings e Fittings connected to the mixing Tee e Fittings connected to the flow sensor outlets 3 With the Analytical Column Equilibration script running monitor flow sensor A Depending on the flow rate do one of the following e If the flow is lt 100 nL
197. g a 2 5 mm L hex wrench insert and tighten the four hex screws that keep the valve in place inside the autosampler compartment In the solvent system compartment restore the tubing and connections to the stator on the valve that was replaced Reinstall the computer box as described in step 1 through step 5 of To install the new computer box on page 112 Mount the left and right side panels to the instrument housing Then with a 2 screwdriver secure each panel by rotating the three captive screws a quarter turn clockwise Run the Flush Air script as described in To run the Flush Air script on page 34 Enter 12 pL for a PLU pump standard in the EASY nLC 1000 instrument e Enter 10 pL for a PLF pump standard in most EASY nLC II instruments Run the Leaks script for the subsystem that you have worked on as described in To run the Leaks script on page 46 e For valve A select A e For valve B select B For valves W or S select System EASY nLC Series Troubleshooting and Maintenance Guide 149 5 Field Service Maintenance Replacing a Pump Replacing a Pump The procedure for replacing a pump differs between EASY nLC 1000 instruments that contain PLU model pumps and EASY nLC II instruments that contain PLF model pumps Note Because Thermo Fisher Scientific no longer stocks the PLF model pump as a replacement part you must replace both pump models with the PLU model pump
198. ge reset valves A and S as follows Valve S 1 2 Valve W 1 2 230 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 6 Troubleshooting Troubleshooting the Results of the System Leak Test b With the Analytical Column Equilibration script running monitor flow sensor A Then depending on the flow rate do one of the following e Ifthe flow is lt 100 nL min you have remedied the leak and completed this troubleshooting procedure Return the instrument to normal operation e Ifthe flow is gt 100 nL min go to the next step 5 Check the Waste In line as follows a Depending on the instrument model do the following e For the EASY nLC II instrument disconnect the Waste In line from the leak test union Then block the free end of the union with a blind fitting e For the EASY nLC 1000 instrument do the following i On the Home gt Overview page change the position of the following valves to return the system to atmospheric pressure Valve S Center Valve W 1 6 ii Using the black knurled nut to loosen the nanoViper fitting disconnect the Waste In line from the leak test union Then block the free end of the union with a blind fitting iii On the Home gt Overview page reset valves S and W as follows Valve S 1 2 Valve W 1 2 Figure 219 shows the Waste In line disconnected from the leak test union and the free end of the union plugged with a blind fitting Thermo Sc
199. gt 100 nL min go to the next step 3 Check if valve W is leaking as follows a On the Home gt Overview page press the valve W icon and set the valve to the Center position Placing valve W in the Center position blocks the solvent flow into valve W and eliminates valve W as the possible source of the leak b With the Analytical Column Equilibration script running monitor flow sensor A Depending on the flow rate do one of the following e Ifthe flow is lt 100 nL min with valve W removed from the solvent path valve W is the source of the system leak To fix the leaking valve follow the instructions in Maintaining the Rotary Valves on page 74 to clean the valve replace the rotor and if necessary replace the stator Then rerun the system leak test e Ifthe flow is gt 100 nL min go to the next step 4 Check for a leak tight connection to port 2 of valve W as follows a Depending on the instrument model do the following e For the EASY nLC II instrument use a 1 4 in open ended wrench to tighten the fitting to port 2 of valve W e For the EASY nLC 1000 instrument do the following i On the Home gt Overview page change the position of the following valves to return the system to atmospheric pressure Valve S Center Valve W 1 6 ii Disconnect and then reconnect the nanoViper fitting from port 2 of valve W Take care to avoid overtightening the fingertight fitting iii On the Home gt Overview pa
200. gure 182 Flow trace for pump B when there is a leak upstream of flow sensor B Met j jitor Mi enanc onf iratior Arrr ir Method Edito Maintenance Configuration Overview Pump B Desired flow Real flow Pressure Flow ni min eal aunssaig Job 00 28 18 Sample 00 28 18 Gradiem 20 00 18 Jul 2011 12 55 admin Paii 5 i i jo 125 150 175 200 225 250 S Exit Time min Negative flow rate during the column equilibration and sample loading steps EASY nLC Series Troubleshooting and Maintenance Guide 175 6 Troubleshooting Troubleshooting Tips Introduced Swept Volume Normally when solvent B acetonitrile with 0 1 formic acid reaches the column the back pressure measured by pressure sensor A drops to less than one half the amount measured for solvent A water with 0 1 formic acid alone If the back pressure does not drop by more than 50 percent when solvent B reaches the column the solvent system might contain additional swept volume introduced by poor tubing connections The additional swept volume increases the gradient delay volume so that the gradient reaches the column later Figure 183 shows the pressure dip that occurs when the gradient starts to reach the column Figure 184 shows the pressure dip for the sample chromatographic method but occurring two minutes later due to the added gradient delay volume Figure 183 Normal pressure dip for pressure sensor A as the gradient reaches the column
201. h e Pressure sensor P N LC502 e 2 5 mm L hex wrench or ball driver CAUTION Wear powder free gloves and safety glasses when handling parts of the LC system that come into contact with solvents To replace a pressure sensor that is connected to a PLU pump follow these steps 1 To remove a pressure sensor from the instrument on page 91 2 To install the new pressure sensor on page 92 90 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 4 Routine Maintenance Replacing a Pressure Sensor for the PLU Pump To remove a pressure sensor from the instrument 1 Set the corresponding valve to the Center position as described in Using the Valve Controls on page 204 2 Close down the EASY nLC system and then turn off the power to the instrument see Closing Down the EASY nLC Instrument on page 21 3 Using a 2 Phillips head screwdriver with a quarter turn loosen the three captive screws that secure the right side panel to the instrument housing Then remove the panel 4 Using a 1 4 in open ended wrench disconnect the stainless steel tubing attached to the inlet and outlet ports of the pressure sensor 5 Using a 2 5 mm L hex wrench or ball driver remove the two screws that secure the pressure sensor L bracket to the instrument panel 6 While holding the pressure sensor use a 2 mm L hex wrench to remove the two screws that secure the pressure sensor to the bracket 7 Disconnect th
202. hat are downstream of valve S to atmospheric pressure 1 On the touch screen press Home gt Overview 2 Using the valve controls set the valves to the following positions Valve Position Effect S Center Maintains the system pressure upstream of valve S W 1 6 Vents the system pressure downstream of valve S Figure 4 shows the Column Out and Waste In solvent lines that are downstream of valve S Figure 4 Solvent lines that are downstream of valve S Column Out line Waste In line Waste beaker Maintaining the PLU Pump Quick Reference Guide Replace the piston seal when you detect a leak in the pump Contents e Removing a Used Piston Seal e Installing a New Piston Seal Replacing a piston seal requires these tools and materials Tools Materials 3 mm hex wrench Pipette Piston seal tool Powder free safety gloves 1 4 in open ended wrench LC MS grade methanol 2 Phillips head screwdriver LC MS grade acetonitrile Piston seal P N LC510 Removing a Used Piston Seal 1 Set the pump piston to the fully retracted position as follows a On the touch screen press Maintenance gt Scripts b In the Category list select Prepare c In the Name list select Purge Solvent d Press the Parameters tab e Press Purge Iterations and enter 0 Figure 1 Purge Solvent script set to 0 iterations Category Narne Prepare a Purge solvent Parameter v Value f Select the check box for the
203. he EASY nLC Instrument Transport Instructions Figure 258 ASA autosampler only Transport collar location transport collars after closing down the instrument 8 Fill out the Declaration of Contamination of Equipment in Declaration of Contamination on page 301 9 Place the EASY nLC instrument in the original shipping container 300 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 9 Returning the EASY nLC Instrument Declaration of Contamination Declaration of Contamination Thermo Scientific To return service or repair the EASY nLC instrument you must complete and submit a Declaration of Contamination of Equipment to Thermo Fisher Scientific with the returned equipment Qualified personnel must review the declaration Contact Thermo Fisher Scientific for additional copies of this form or if you have any questions regarding the contents of this declaration Description of equipment Equipment type model Serial No Date of receiving purchasing equipment Reason for return Maintenance Repair End of test Describe symptoms and problems Equipment condition Has the equipment been used Yes No Has the equipment been exposed to potentially harmful substances Yes No If yes attach list of all known harmful substances including chemical name and symbol and precautions associated with the substances Were any of the harmful substances
204. he EASY nLC instrument 21 U union HPLC 218 upgrading the touch screen software 128 296 URLs consumable parts xxiii customer manuals xx usage counters device 56 USB cable internal 114 USB ports EASY nLC instrument 17 293 USB storage device copying system files 293 user interface layout 19 Vv Valve Check script 47 valve position description 12 manual control 204 Valve Tune script 50 Thermo Scientific Index T valves check valves description 11 replacing 80 checking for blockage valve B 257 valves A S and W 251 checking for leaks valve B 223 rotary description 12 maintaining 74 replacing 147 versions application 20 297 vials extra vial positions calibrating 285 plate format calibrating 280 viscosities solvent 311 W wash bottles calibrating W4 bottle with insert 285 description 2 waste beaker description 2 Waste In line checking for blockage 253 weak signal from detector 183 WEEE compliance ix weight EASY nLC instrument 25 well plates calibrating 280 plate formats managing 274 X Xcalibur data system errors reported by 189 XYZ robot calibrating 278 286 direct control 210 XYZ axis PCB 123 127 129 132 XYZ axis PCB bus cable 123 EASY nLC Series Troubleshooting and Maintenance Guide 339
205. he Gradient on page 180 e Sample Signal Weak or Absent on page 183 e Slow or No Pressure Increase in Subsystem A or B on page 187 e Errors Reported by the Xcalibur Data System on page 189 e Carryover on page 194 e Spray Issues on page 196 e Chromatographic Performance on page 197 e Device Failures on page 198 e Miscellaneous on page 202 170 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific Autosampler Problems 6 Troubleshooting Troubleshooting Tips Table 9 lists troubleshooting tips for the autosampler Note For information about troubleshooting sample pickup see Troubleshooting the Autosampler Aspiration and Calibration on page 261 Table 9 Autosampler troubleshooting tips Symptom The needle cannot penetrate the plastic film on the microtiter plate Possible causes The needle tip is damaged or bent Action Do the following 1 Check the software configuration for the adapter tray 2 Eject the tray 3 Remove the left side panel and visually inspect the needle tip 4 Do one of the following e Ifthe needle tip is bent or damaged replace it see Replacing the Autosampler Needle on page 84 Ifthere is any residue on the needle tip wipe the needle tip with a lint free tissue soaked in LC MS grade ethanol XYZ robot error Possible step loss on one of the motors because the movement of the
206. he Latest Firmware File 0 00 00 ce eee eee 296 Chapter9 Returning the EASY nLC Instrument 0 0 0 cece eee eee eee 299 Transport Instructions s es aiden tossed Ante wr Sew aa eaten tlee a ehh eae y Maes 299 Declaration of Contamination 44s led dale beers ee on ee aad 301 xvi EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific Thermo Scientific Appendix A Appendix B Appendix C Appendix D Contents Error Godesk nenna E aa face ewe ware E a EE EE 303 Mobile Phase Viscosity 0c ccc cee cece eee anann 311 Consumables and Replacement Parts 0 0 eeeeeeeee eens 313 Ordering Informations i othe erie xe Poe lea ah hn bond Repeat 313 Solvent System Schematic for the EASY nLC II Instrument 314 Solvent System Schematic for the EASY nLC 1000 Instrument 316 Common Replacement Parts o35 02fasseshe dda cadens te hiatn thea cy atecartstal eck 318 Common Replacement Parts for the EASY nLC I Instrument 318 Common Replacement Parts for the EASY nLC 1000 Instrument 319 Quick Reference Guides for Routine Maintenance 5 321 Using nanoViper Fittings Quick Reference Guide 0 004 323 Connecting nanoViper Fittings 02 0 eee ee eee ee 323 hanoViper Tubing Connections 2 i aii nsec ben 2 ach ad aay ntetintie neee 324 Returning the System to Atmospheric Pressure 0 0000000 325 Maint
207. he pump is filling and the flow to the waste beaker when the pump is emptying see Prepare Purge Solvent on page 32 Replacing an Inline Filter for the EASY nLC 1000 Instrument Inline filters are connected to both the inlet and outlet ports of the flow sensors The inline filter consists of a stainless steel body nut filter and tube and a ferrule see Figure 69 Figure 69 Inline filter components nut with integrated tube and filter and ferrule all Replacing an inline filter requires the following tools and materials Tools Parts and materials e 5 16 in open ended wrench e Powder free safety gloves e 2 Phillips head screwdriver e Inline filter CAUTION Wear powder free gloves and safety glasses when handling parts of the LC system that come into contact with solvents To install an inline filter into the receiving port of a flow sensor 1 Close down the EASY nLC system and then turn off the power to the instrument see Closing Down the EASY nLC Instrument on page 21 2 Using a 2 Phillips head screwdriver with a quarter turn loosen the three captive screws that secure the right side panel to the instrument housing Then remove the panel Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 81 4 Routine Maintenance Replacing an Inline Filter for the EASY nLC 1000 Instrument 3 While holding the wide end of the ferrule toward the nut slide the ferrule over the tube that extend
208. he valve with the replacement stator If you replaced the stator on Valve S or W run the Leaks script for the system 332 Replacing a Damaged Autosampler Needle Replace the autosampler needle when it is bent or clogged Contents e Removing the Damaged Autosampler Needle e Installing a New Autosampler Needle A Replacing a damaged needle requires these tools and materials CAUTION Wear powder free safety gloves when working with solvents and handling the wetted components of the instrument Tools Materials e 1 4in open ended wrench Powder free safety gloves e 2 Phillips head screwdriver ASA autosampler needle P N LC251 e ASC autosampler needle P N LC302 Removing the Damaged Autosampler Needle To remove the autosampler needle 1 Using a 2 Phillips screwdriver with a quarter turn loosen the three captive screws that secure the panel to the right side the EASY nLC instrument and then remove the panel 2 Using a 1 4 in open ended wrench unscrew the nut that secures the autosampler needle to port 1 of valve S Then remove the fitting from the port Figure 1 Valve S solvent line connections for the EASY nLC II system Valve S Autosampler needle Column Out line From the EEE pressure From the sensor mixing Tee Figure 2 Valve S solvent line connections for the EASY nLC 1000 system From the Autosampler pressure needle sensor From the
209. heck valve and then terminate the solvent line with a plug e Fora waste side check valve remove the waste line from the check valve CAUTION To prevent solvent from siphoning out of the solvent bottle on top of the instrument and onto system components use a plug to terminate a solvent inlet line when you disconnect it from the solvent side check valve The pump PCB is easily damaged by contact with solvents 4 Do one of the following e Fora solvent side check valve use a 9 16 in open ended wrench to loosen the valve from the check valve assembly e For the waste side check valve remove the check valve by turning it counterclockwise with your fingers 5 Screw the new check valve onto the check valve assembly Using a 9 16 in open ended wrench slightly tighten the solvent side check valve to the assembly 80 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 4 Routine Maintenance Replacing an Inline Filter for the EASY nLC 1000 Instrument 6 Do one of the following e Fora solvent side check valve reconnect the solvent inlet line e Fora waste side check valve reconnect the waste line 7 Mount the right side panel to the instrument housing Then with a 2 Phillips head screwdriver secure the panel by rotating the three captive screws a quarter turn clockwise 8 Run the Purge Solvent script for the pump associated with the replaced check valve and monitor the flow from the solvent bottle when t
210. hex wrench to loosen the screws on the front see Figure 146 Figure 146 Screws on the front of the EASY nLC II instrument model LC100 2 O O 4 Using a 2 Phillips head screwdriver with a quarter turn loosen the three captive screws that secure the left side panel to the instrument housing Then remove the panel Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 141 5 Field Service Maintenance Replacing the Autosampler Cooler 5 Using a flathead screwdriver or your fingernail loosen the cap that secures the ribbon cable to the controller PCB as shown in Figure 147 Figure 147 Loosening the ribbon cap on the controller PCB Figure 148 shows a close up view of the cap that secures the cable to the PCB Figure 148 Cap around the ribbon cable close up view 6 Pull the ribbon cable out of the cap as shown in Figure 149 Figure 149 Pulling the ribbon out of the cap 142 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 5 Field Service Maintenance Replacing the Autosampler Cooler 7 Using a 2 5 mm L hex wrench remove the lid where the ribbon cable connects to the autosampler tray see Figure 150 Figure 150 Removing the lid 8 Using a 2 mm hex wrench remove the screws in the small PCB underneath the lid Figure 151 shows the screw in the lower right corner and Figure 152 shows the screw in the middle of the left side Figure 151 Loosening the scre
211. hooting Troubleshooting the Results of the System Leak Test b With the Analytical Column Equilibration script running monitor flow sensor A Then depending on the flow rate do one of the following e If the flow is lt 100 nL min reconnect the solvent lines and rerun the system leak test as described in Running a System Leak Test on page 218 to confirm that the system is leak tight e Ifthe flow is gt 100 nL min go to the next step 7 Determine whether the leak is in the Column Out line or in valve S as follows a Depending on the instrument model do the following e For the EASY nLC II instrument use a 1 4 in open ended wrench to disconnect the Column Out line from port 3 of valve S Plug the port with a blind fitting Then use a 1 4 in open ended wrench to tighten the fitting e For the EASY nLC 1000 instrument do the following i On the Home gt Overview page set valve S to the Center position to return the system to atmospheric pressure ii Using the black knurled nut disconnect the Column Out line from port 3 of valve S Then block port 3 of valve S with a blind fitting iii On the Home gt Overview page reset valve S to position 1 2 Figure 220 shows port 3 of valve S plugged with a blind fitting Figure 220 Port 3 of valve S plugged with a blind fitting Valve S Valve W From the mixing Tee Blind fitting in port 3 of valve S Thermo Scientific EASY nLC Series Troubleshooting and Maintenance
212. ide Thermo Scientific Thermo Scientific 7 Calibrating the Autosampler s XYZ Robot Preparing the Autosampler for the Calibration Routines 4 Install the wash bottle in position W4 IMPORTANT For the autosampler to determine the appropriate depth for the needle the wash bottle in position W4 must contain the needle wash insert 5 Remove the left side panel so that you can watch the movement of the needle To calibrate the wash bottle and extra vial positions go to Calibrating the Wash Bottle and Extra Vial Positions on page 285 EASY nLC Series Troubleshooting and Maintenance Guide 279 7 Calibrating the Autosampler s XYZ Robot Calibrating Plates Calibrating Plates For information about selecting or creating a plate format see Managing Plate Formats on page 274 IMPORTANT When calibrating the XYZ robot for vial or microwell plates make sure to remove the vial caps or plate covers Keeping the caps or covers on makes the needle susceptible to bending During a sample run the z axis needle holder moves to the specified sample position along the xy plane When the z axis needle holder reaches the specified location the needle descends along the z axis into the sample vial or well Calibrating plates requires determining the xyz positions for the top left and bottom right vial or well Table 27 lists the top left and bottom right positions for the three plate formats Table 27 Top left and b
213. ientific EASY nLC Series Troubleshooting and Maintenance Guide 231 6 Troubleshooting Troubleshooting the Results of the System Leak Test Figure 219 Setup for eliminating the Waste In line as the source of the system leak Blind fitting Valve S Valve W From the mixing Tee b With the Analytical Column Equilibration script running monitor flow sensor A Then depending on the flow rate do one of the following e If the flow is lt 100 nL min install a new Waste In line Then reconnect the solvent lines and rerun the system leak test as described in Running a System Leak Test on page 218 For ordering information see Appendix C Consumables and Replacement Parts e Ifthe flow is gt 100 nL min go to the next step 6 Check for a leak tight connection to port 3 of valve S as follows a Depending on the instrument model do the following e For the EASY nLC II instrument use a 1 4 in open ended wrench to tighten the fitting to port 3 of valve S e For the EASY nLC 1000 instrument do the following i On the Home gt Overview page set valve S to the Center position to return the system to atmospheric pressure ii Disconnect and then reconnect the Column Out line to port 3 of valve S Take care to avoid overtightening the fingertight fitting iii On the Home gt Overview page reset valve S to position 1 2 232 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 6 Troubles
214. ight need to wiggle the connectors carefully from side to side to loosen them Note Take care when you disconnect the monitor LVDS cable because it is easily damaged 108 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific Thermo Scientific 5 Field Service Maintenance Replacing the Built In Computer Figure 97 shows these cables in the EASY nLC 1000 instrument and Figure 98 shows these cables in the EASY nLC II instrument Figure 97 Cables connected to the computer box in the EASY nLC 1000 instrument LVDS cable LVDS cable Tip To remove the 12 V cables on the EASY nLC II instrument press the tab on the top of the plug as shown in Figure 99 Figure 99 Pressing the tab on the top of the plug of the 12 V cables EASY nLC Series Troubleshooting and Maintenance Guide 109 5 Field Service Maintenance Replacing the Built In Computer 5 Using a Torx T 10 wrench remove the screws that secure the back panel to the instrument see Figure 100 Note In older EASY nLC instruments 11 screws secured the back panel to the housing In current EASY nLC 1000 instruments 3 screws secure the back panel to the housing Figure 100 Screws that secure the back panel to the instrument housing M omm OA LAN MAC ADDRESS 00 6 0 45 4E 28
215. ilure messages 216 fan cables 145 Fast Inspection option 52 FCC compliance iii field service repairs See EASY nLC II instrument or EASY nLC 1000 instrument service repairs filling a pump with solvent 206 flow rate determining appropriate analytical column 36 precolumn 34 direct control 205 measuring 10 flow sensor cable 152 163 166 flow sensors calibration script 51 checking for blockage 255 259 description 10 maintenance schedule 29 replacing EASY nLC 1000 instrument 92 EASY nLC II instrument 96 Flush Air script 33 fuse replacing 58 G grounding requirements 268 H hard drive replacing 100 HPLC union 218 Thermo Scientific inline filters replacing in the EASY nLC 1000 instrument 81 Inspection Only check box 52 instrument startup troubleshooting 172 interface layout 19 IP address support server 289 Isocratic Flow script 38 L L brackets pressure sensor 91 120 157 Leaks script 44 46 leaks checking for 28 44 46 218 247 log book 55 Logo icon Thermo Scientific 20 loop sample replacing 89 lower case letters cause of Acquisition Server error 189 low pressure solvent filters 29 LVDS cable internal 114 116 maintenance daily 27 quarterly 28 scripts 31 weekly 28 yearly 29 manuals EASY nLC xx mass spectrometer contact closure MS Connection script 39 troubleshooting 172 266 memory stick correct format 202 message log file 292 messages failure 216 mixing Tee checking for block
216. in The cause of this test result is a leak between flow sensor A and the inside of pump A The system leak test script has detected that the piston movement is 1000 nL min more than the flow measured at flow sensor A This procedure checks whether the leak is in the solvent path between the valve and the flow sensor If the leak in not between the valve and the flow sensor this procedure directs you to the Troubleshooting a Pump that Fails the Leaks Script on page 216 where you determine if the leak is coming from the solvent path between the pump and the valve or from the pump itself Figure 214 shows the solvent path from pump A to flow sensor A Figure 214 Solvent path from pump A to flow sensor A Pump to pressure sensor Pressure sensor to valve A Pump A o Valve to flow sensor Flow sensor A 224 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 6 Troubleshooting Troubleshooting the Results of the System Leak Test To identify a leak in pump A or the solvent path between pump A and flow sensor A 1 Run the Leaks script for pump A only as follows a Set the parameters for the Leaks script as follows i If you exited the Leaks script on the Maintenance gt Scripts page reopen it ii Press the Parameters tab iii In the Value list select A pump A b Press Start 2 Depending on whether the leak test for pump A passes or fails do the following Ifthe script passes the leak is i
217. ing moves up a bit or gently hold needle tubing as you press the step button 282 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 7 Calibrating the Autosampler s XYZ Robot Calibrating Plates Tip For the ASA autosampler do one of the following to determine the bottom position of the vial or well e To feel when the needle reaches the bottom of the vial or well hold the needle gently as you press the down arrow on the touch screen interface To see when the needle reaches the bottom of the vial or well draw a mark on the needle and observe the needle mark as you press the down arrow on the touch screen interface When the needle mark moves up a bit the needle has reached the bottom of the vial Figure 247 shows the ASA needle holder and needle Figure 247 ASA autosampler needle holder ar Ea Use a black marker pen to make a line on top of the PEEKsil tubing c Press the small step button once to raise the needle slightly off the bottom of the vial or well The appropriate needle height is one small step higher than the bottom of the vial or well 6 When you are satisfied with the current xyz coordinate for the vial or well press Capture The color of the vial or well on the plate graphic changes from green to red and the position readbacks for the xyz axes are unpopulated see Figure 248 Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 283
218. ing the Rotary Valves Replacing the Rotor Seal If cleaning the rotor seal and stator does not remove the system leak replace the rotor seal Replacing the rotor seal requires one of these parts Valve serial number Replacement part V 010000 and above Rotor seal P N LC228 V 009999 Rotor seal P N LC224 Tip The About page of the Rotary Valve view on the Maintenance gt Devices page lists the serial number of the selected valve To replace the rotor seal in a valve and return the instrument to normal operation follow these steps 1 To remove the stator from the valve on page 75 2 To replace the rotor seal on this page To reset the usage counter for the valve on page 78 A GB To remove air from the system and check for leaks after disassembling a valve on page 76 To replace the rotor seal 1 If you have not already done so access the rotor seal by following To remove the stator from the valve on page 75 2 Remove the rotor seal from the valve driver see Figure 68 Figure 68 Rotor seal mounted on the valve driver Rotor seal 3 Carefully mount the new rotor onto the valve driver ensuring that the rotor sealing surface engraved flow passage is facing out 4 If you have not already done so clean the stator with a lint free tissue or Q tip soaked in methanol 5 Mount the clean stator onto the valve driver Thermo Scientific EASY nLC Series Troubleshooting and
219. inst each other inside the union do not tighten them by more than 90 degrees 1 4 turn Overtightening the fittings can irreparably damage their sealing surfaces Figure 212 shows the connection between the Column Out and Waste In lines for the EASY nLC 1000 instrument Figure 212 Column Out line connected to the Waste In line EASY nLC 1000 instrument jac he nanoViper Union fitting P N SC900 IMPORTANT Follow the instructions in Using nanoViper Fittings on page 83 to ensure that the nanoViper fittings are securely connected to the union 3 Open the Test Leaks script as follows a Press Maintenance gt Scripts b In the Category list select Test c Inthe Name list select Leaks 4 Set the parameters for the system leak test as follows a Press the Parameters tab b In the Value list select System 5 Press Start The script starts and the Output page opens During the duration of this script the EASY nLC instrument pressurizes the flow paths highlighted with a dotted red line in Figure 35 on page 45 and it monitors the flow sensors and pump piston movements to identify and localize leaks When the script ends the Output page displays a message 6 Depending on the message displayed on the Output page of the Test Leaks script take the appropriate action as listed in Table 24 220 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 6 Troubleshooting Troubleshooting the Result
220. instrument 5 Insert the P Bus terminator between the computer box and the P Bus communication cables as shown in Figure 127 It does not matter which cable the terminator connects to Note Do not install the connector if the computer box is labeled Terminated as shown in Figure 128 This designation means that you do not have to install a separate terminator cable Figure 127 P Bus connector inserted between the computer box and the P Bus communication cables P Bus connector Figure 128 Computer box with Terminated label Terminated label Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 131 5 Field Service Maintenance Upgrading from an ASA Autosampler to an ASC Autosampler 6 Using a Torx T 10 wrench and the four screws that you removed in step 6 on page 111 secure the computer box to the instrument see Figure 101 on page 111 7 Connect the P Bus communication cables to the autosampler The longer cable connects to the cooler PCB The shorter cable connects to the XYZ axis PCB Figure 129 shows these two cables Figure 129 P Bus communication cables XYZ BOARD Figure 130 shows the boards that the P Bus communication cables connect to Figure 130 Boards that the P Bus communication cables connect to Cooler PCB XYZ axis PCB 8 Using a 2 5 mm hex wrench or ball driver mount valve W 132 EASY nLC Series Troubleshooting and Maintenance Guide Ther
221. inted circuit board behind the LED panel 8 Reinstall the right side panel 9 Reset the usage counter for the pump as follows a Press Maintenance gt Devices b Select the pump from the list of devices c Press the Summary tab d Press Reset The value in the Intermediate Volume box resets to 0 10 To draw fresh solvent through the solvent lines and to remove air do the following a Open the Parameters page for the Purge Solvent script as described in step 1 on page 327 b In the Purge Iterations box enter 10 c Select the check box for the appropriate pump d Press Start and wait for the system to perform 10 purge cycles 11 To remove air from the pump head do the following a In the Name category on the Maintenance gt Scripts page select Flush Air b Press the Parameters tab c In the Flush Threshold uL box enter 12 d Press Start e Wait for the script to finish 12 Run the Leaks script for the pump with the new piston seal a On the Maintenance gt Scripts page select Test in the Category list and then select Leaks in the Name list b Press the Parameters tab c Select the check box for the pump with the new seal d Press Start 13 When the instrument is leak tight it is ready for use Maintaining the PLF Pump Quick Reference Guide Replace the piston seal when you detect a leak in the pump Contents Removing the Pump Head and Cleaning the Piston Removing the Worn P
222. ion Cycle EASY nLC power NO_DATA flow sensor for X seconds Check the flow sensor functionality 35 ERROR_SENSIRION_ Flow sensor in Trying to startaflow Cycle EASY nLC power IN_MEASURE_MODE measurement mode Stop sensor that is already measurements before started sending further commands 36 ERROR_SENSIRION_ IO Error while see issue description Check the flow sensor IO communicating with flow communication cable sensor 37 ERROR SENSIRION_ No response from flow see issue description Cycle EASY nLC power TIMEOUT sensor timeout If the problem persists replace the flow sensor See Replacing a Flow Sensor on page 92 38 ERROR SENSIRION_ Flow sensor echo Flow sensor fails Rare error Connect the ECHO_MISMATCH ECHO does not match to talk back EASY nLC system to command remote support COMMAND See Connecting the EASY nLC Instrument to the Support Server on page 288 39 ERROR SENSIRION_ Flow sensor error Flow sensor failing but Rare error Connect the BAD_RESPONSE ERROR_MESSAGE Flo does not report it EASY nLC system to w sensor did not respond remote support with OK but did not report an error either 40 ERROR_SENSIRION_ Cannotcalibrate sensorin Flow sensor in idle Cycle EASY nLC power IN_IDLE_MODE idle mode mode during Check the flow sensor calibration functionality Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 305 A Error Codes Table 30 Error codes Sheet 4 of 8
223. iston Seal Installing a New Piston Seal Priming the Pump MN mB WO NHN Preparing the Instrument for Operation A Replacing a piston seal requires these tools and materials CAUTION Wear powder free safety gloves when working with solvents and handling the wetted components of the instrument Tools Materials 3 mm hex wrench Pipette Piston seal tool Powder free safety gloves LC MS grade methanol 2 Phillips head screwdriver LC MS grade acetonitrile Piston seal P N LC210 includes the piston seal tool 1 4 in open ended wrench Removing the Pump Head and Cleaning the Piston 1 Set the pump piston to the fully retracted position as follows a On the Maintenance gt Scripts page Select Prepare in the Category list and then select Purge Solvent in the Name list Press the Parameters tab In the Purge Iterations box enter 0 Select the check box for the appropriate pump b Press Start The piston moves down to the 140 pL position 2 Exit the EASY nLC II system and turn off the power 3 Using a 2 Phillips head screwdriver with a quarter turn loosen the three captive screws that secure the right side panel to the instrument Then remove the panel 4 Remove the tubing connected to the pump head as follows e Ifa PEEK fitting is connected to the pump head use a 13 mm open ended wrench to remove it Figure 1 PEEK fitting on the externally threaded pump head PEEK fitting connected to an externally th
224. it Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 49 3 Maintenance Scripts and Service Records Maintenance Scripts Test Pump Torque For the PLU pump in the EASY nLC 1000 instrument the Pump Torque script measures the actuators ability to generate pressure at predefined torque levels IMPORTANT Do not use this script for the PLF pump in the EASY nLC II instrument Contact your local Thermo Fisher Scientific representative before running this script Figure 39 shows the parameters for the Pump Torque script Figure 39 Pump Torque script parameters Thermo Home Batch Setup Method Editor Maintenance Configuration SCIENTIFIC cripts Category Name f fi _ No graph X Log Book Test Pump torque Support peeo Parameters elas Devices fro graph Job 00 15 48 11 May 2011 12 51 admin Calibrate Valve Tune The Valve Tune script automatically retunes the selected valve in the EASY nLC II instrument IMPORTANT This script is only valid for an EASY nLC II valve with a serial number below V 009999 Contact your local Thermo Fisher Scientific representative before running this script as running this script incorrectly will compromise the performance of the instrument 50 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 3 Maintenance Scripts and Service Records Maintenance Scripts Calibrat
225. itter to the Column Out tubing Figure 42 shows an example of a direct infusion setup for an EASY nLC 1000 instrument Figure 42 Setup for a direct infusion experiment 1 32 in fitting Column Out line PEEK adapter union for 1 16 in OD and 1 32 in OD tubing Emitter and sleeve 1 16 in fitting 2 Mount the assembly onto your nanoflow ion source Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 53 3 Maintenance Scripts and Service Records Maintenance Scripts Torun the Direct Infusion script 1 Select the Direct Infusion script as follows a Press Maintenance gt Scripts b In the Category list select Calibrate c Inthe Name list select Direct Infusion 2 Set up the parameters for this script as follows a Press the Parameters tab b In the Pickup Volume pL box enter the volume that you want the autosampler needle to pick up The autosampler loads this volume into the sample loop c In the Pickup Flow pL min box enter the flow rate at which pump S aspirates the sample from the vial or well d In the Pickup Position box enter the vial or well position e In Infusion Volume box pL enter the volume that pump A delivers to the system to backflush the sample from the sample loop out through the Column Out line and into the mass spectrometer s ion source f In the Infusion Flow pL min box enter the flow rate at which pump A moves the sample into the mass spectrometer s
226. k of the pump PCB see Figure 178 Figure 178 Motor cable connected to the pump PCB PLU model pump Motor cable Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 165 5 Field Service Maintenance Replacing a Pump PCB Nn 10 1 j To replace a pump PCB on the PLU model pump Using a small flathead screwdriver reset the address on the rotary switch on the pump PCB see Figure 179 as follows e Pump A 1 e Pump B 2 e Pump S 3 Figure 179 PCB rotary switch PCB rotary switch Plug the motor cable into the back of the PCB see Figure 178 on page 165 Insert the new pump PCB onto the pump Install the PCB shield Using a 2 5 mm L hex wrench or ball driver tighten the four 2 5 mm hex screws in the PCB shield Reconnect the P Bus communication cables the flow sensor cable and the pressure sensor cable see Figure 176 on page 164 to the pump Mount the right side panel to the instrument housing Then with a 2 screwdriver secure the panel by rotating the three captive screws a quarter turn clockwise Reconnect the power cable to the back panel of the instrument Turn on the instrument Wait for 30 minutes to allow the temperature in the housing to stabilize Calibrate the flow sensor by following the automated script described in Calibrate Flow Sensors on page 51 166 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific
227. lacing do the following e When replacing pump A or B run the pump leak test and then run the flow sensor calibration see Test Leaks on page 44 and Calibrate Flow Sensors on page 51 e When replacing pump S run the sample pickup test see Test Sample Pickup on page 42 Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 153 5 Field Service Maintenance Replacing a Pump Replacing a PLF Pump with a PLU Pump You must replace an irreparably damaged PLF pump in an EASY nLC II instrument with a PLU pump as Thermo Fisher Scientific no longer stocks the PLF pump as a replacement part When you replace a PLF pump with a PLU pump do the following Replace the pressure sensor for the pump and the high pressure tubing associated with the pump as the PLU pump is not compatible with the pressure sensor used in the EASY nLC II instrument Upgrade the touch screen software to version 3 1 4 or later as the EASY nLC II instrument only supports the PLU pump when running software version 3 1 4 or later If you are replacing pump S in an EASY nLC II instrument with an earlier version of the chassis order and install the components in the Pump Mounting Kit so that the PLU pump can fit into the pump S position Replacing an EASY nLC II pump PLF model with a PLU pump requires these items Tools Parts and materials 1M kod l 2 Phillips screwdriver e Powder free gloves 1 4 in
228. le 2 lists the function of each back panel element Table 2 Back panel elements Element Description RS 232 For communication with add on devices for example a syringe drive USB Port for keyboard or mouse or removable USB storage device two 2 provided PS 2 discontinued computer box only Input for connection of keyboard and mouse LAN For 10 100 MB sec Ethernet connection MONITOR Output for connection of external display P BUS For communication with add on devices IN OUT pins Contact closure primarily for communication with the mass spectrometer IJO Power switch Fuse holder below Replaceable fuse ratings IJO switch o site For 120 V T5 AL 250 V e For 230 V T 2 5 AL 250 V P N Product order code S N Instrument serial number LAN MAC ADDRESS MAC address of the embedded computer Gives the EASY nLC computer a unique network identifier on your local network EASY nLC Series Troubleshooting and Maintenance Guide 17 1 Introduction Touch Screen Application Touch Screen Application The EASY nLC instrument includes an integrated computer with instrument control software that you access from the instrument monitor These topics provide an overview of the touch screen application and describe how to log in to the EASY nLC instrument as an administrator and how to close down the instrument e Description of the Touch Screen Monitor e Logging In to the EASY nLC Instrument for Mainten
229. le 21 lists troubleshooting tips for devices failures Table 21 Troubleshooting tips for device failures Symptom Possible cause Action The pump icon fora pump is Communication failure Check that the that is not functioning turns 140 0 ul to the pump cables are a pink color on the Home gt connected Overview page Could be the pump or correctly the PC BUS cable See Figure 204 on page 199 The valve icon for a valve Communication failure Check that all of that is not functioning turns to the valve the cables are a pink color on the Home gt connected Overview page Could be the valve or correctly the PC BUS cable See Figure 205 on page 200 The autosampler icon turns Communication failure Check that all of a pink color on the Home gt on the cooling unit the cables are Overview page connected Could be the PC box a correctly See Figure 206 on page 201 198 EASY nLC Series Troubleshooting and Maintenance Guide damaged cable or a loose connection Thermo Scientific Thermo Scientific 6 Troubleshooting Troubleshooting Tips A pump icon turns pink when the pump loses communication with the EASY nLC computer The pumps are serially connected so if pump B loses communication all of the pumps lose communication Pump B is the first pump in the series Communication between the pumps and the embedded computer can be lost when the cables are loose when the embedded computer fails or when the pump PCB fails
230. lication Power Down Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 21 1 Introduction Touch Screen Application 2 Press Power Down The EASY nLC application displays a white screen with a small progress bar When the progress bar is filled out and the message appears indicating you can safely turn off the instrument go to the next step EAS Y nLC Please wait SCIENTIFIC 3 After receiving the message that you can safely turn off the instrument turn off the power switch on the back panel of the instrument 22 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 1 Introduction Specifications Specifications These topics describe the performance and technical specifications for the EASY nLC syringe pumps and autosampler e Performance Specifications e Technical Specifications Performance Specifications Table 3 lists the performance specifications for the EASY nLC instruments Table 3 Performance specification Sheet 1 of 2 Item Specification Flow range gradient 20 to 2000 nL min Recommended 100 to 1000 nL min Flow while loading and Up to 25 pL min equilibrating Pressure range EASY nLC II 0 to 300 bar EASY nLC 1000 0 to 1000 bar Retention time Within the recommended flow range 0 1 to 0 4 reproducibility RT RSD Outside the recommended flow range typically better than 1 Peak widths Typically 3 to 5 seconds full width at half maxi
231. llowing Ifthe pump does not aspirate solvent go to step 6 e Ifthe pump aspirates solvent the tubing that connected the pressure sensor to the valve is damaged Do the following i Using a 1 4 in open ended wrench disconnect this tubing from the valve and install a new solvent line ii To confirm that installing new tubing fixes the problem run two iterations of the Purge Solvent script Then run the Flush Air script with a threshold of 10 nL for the PLF pump or 12 pL for the PLU pump 6 To determine if the problem is in the pump do the following a Reconnect the solvent line to the top of the pressure sensor and tighten the fitting b Disconnect the solvent line from the pump head by doing one of the following e Ifa PEEK fitting is connected to the pump head use a 13 mm open ended wrench to disconnect it e Ifa stainless steel fitting is connected to the pump head use a 1 4 in open ended wrench to disconnect it c Pipette the appropriate solvent into the port in the pump head see Figure 65 on page 71 214 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 6 Troubleshooting Troubleshooting a Pump that Fails the Flush Air Script d Run the Purge script and check that the pump is aspirating solvent from the pump head into the pump e Ifthe pump does not aspirate solvent from the pump head the problem is in the pump e Ifthe pump does aspirate solvent from the pump head replace the
232. max pressure are specified the pump flow is limited by whichever parameter is reached first CAUTION Check the maximum pressure rating for the precolumn Running the instrument at pressures higher than the column s maximum pressure rating reduces the column lifespan 3 Press Start EASY nLC Series Troubleshooting and Maintenance Guide 35 3 Maintenance Scripts and Service Records Maintenance Scripts Prepare Analytical Column Equilibration The Analytical Column Equilibration script equilibrates the analytical column with solvent from pump A Use this script to equilibrate or flush the analytical column determine a suitable equilibration flow rate for the method or initialize a new analytical column Figure 27 shows the parameters for the Analytical Column Equilibration script Figure 27 Analytical Column Equilibration script parameters Thermo Home Batch Setup Method Editor Viaintenance i teleiteieieialeya SCIENTIFIC fe Famers p eaten Log Book repare ae E nalytical col e v Support Devices _ No graph Job 00 05 02 Sample 00 05 00 Gradient 00 00 2 May 2011 14 06 admin Schedule Exit Note The Analytical Column Equilibration script sets the valves to these positions e Valve A 1 6 e Valve B Center e Valve S 1 2 e Valve W 1 2 36 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific Thermo Scientific 3 Maintenance Scripts
233. micos o particulas que salten bruscamente pueden causar lesiones en los ojos Utilice anteojos protectores al manipular productos qu micos o al darle servicio de mantenimiento al instrumento Peligro general Significa que existe un peligro no incluido en las categorias anteriores Este simbolo tambi n se utiliza en el instrumento par referir al usuario a las instrucciones contenidas en este manual Cuando la certidumbre acerca de un procedimiento sea dudosa antes de proseguir pongase en contacto con la Oficina de Asistencia Tecnica local para los productos de Thermo Fisher Scientific San Jose AVVERTENZA Shock da folgorazione L apparecchio alimentato da corrente ad alta tensione che puo provocare lesioni fisiche Prima di effettuare qualsiasi intervento di manutenzione occorre spegnere ed isolare l apparecchio dalla linea elettrica Non attivare lo strumento senza lo schermo superiore Non togliere i coperchi a protezione dalle schede di circuito stampato PCB Prodotti chimici Possibile presenza di sostanze chimiche pericolose nell apparecchio Indossare dei guanti per maneggiare prodotti chimici tossici cancerogeni mutageni o corrosivi irritanti Utilizzare contenitori aprovo e seguire la procedura indicata per lo smaltimento dei residui di olio Calore Attendere che i componenti riscaldati si raffreddino prima di effetturare l intervento di manutenzione Incendio Adottare le dovute precauzioni quan
234. mo Scientific 5 Field Service Maintenance Upgrading from an ASA Autosampler to an ASC Autosampler 9 Secure the autosampler to the housing as follows a Using a pair of tweezers place the M5 x 16 hex screw in the back right mounting position lifting up the autosampler in order to put the screw in place Figure 131 Figure 132 and Figure 133 demonstrate this procedure Figure 131 Lifting up the autosampler to position the screw Figure 132 Placing the M5 x 16 hex screw into the back right side of the autosampler frame Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 133 5 Field Service Maintenance Upgrading from an ASA Autosampler to an ASC Autosampler b Insert the screw into the back left side of the autosampler Use a ball driver to tighten the screw see Figure 134 Figure 134 Securing the back left screw c Insert the screw into the front of the autosampler Use a ball driver to tighten the screw see Figure 135 Figure 135 Securing the front screw 134 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 5 Field Service Maintenance Upgrading from an ASA Autosampler to an ASC Autosampler 10 Place the air filter from the ASA autosampler onto the ASC cooler see Figure 136 Figure 136 Placing the air filter onto the cooler Because the injection needles are different for the ASA and ASC autosamplers you must install a needle arm that can be held in
235. most of the instrument components by removing either the left or right side panel of the instrument Three captive quarter turn screws secure each panel to the instrument housing To remove a side panel from the housing loosen unlock the screws from the housing by rotating them 90 counterclockwise with a 2 Phillips head screwdriver When loosened the screws rotate freely without constraint but remain connected to the side panel e To secure a side panel to the housing mount the panel Then tighten the screws to the housing lock by rotating them 90 clockwise with a 2 Phillips head screwdriver For information about the suggested maintenance schedule and the built in maintenance scripts and record keeping features provided by the EASY nLC software see Chapter 3 Maintenance Scripts and Service Records Contents e Maintaining a Clean Working Environment e Replacing the Main Power Fuse e Maintaining the Syringe Pumps e Maintaining the Rotary Valves e Replacing the Check Valves e Replacing an Inline Filter for the EASY nLC 1000 Instrument e Using nanoViper Fittings e Replacing the Autosampler Needle e Replacing the Sample Loop e Replacing a Pressure Sensor for the PLU Pump e Replacing a Flow Sensor e Replacing the Hard Drive e Managing the Devices List Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 57 4 Routine Maintenance Maintaining a Clean Working Environment Maintaining a Clean Working
236. mps are ejecting solvent make sure that solvent is exiting the A and B waste tubing into the waste beaker e When the pumps are refilling make sure that no solvents are going into the pump through the waste tubing If you detect a leaking check valve replace it Yearly Maintenance Replace the solvent filters and check the flow sensor calibration once a year 1 To exchange low pressure solvent filters and high pressure inline filters Thoroughly clean new solvent filters before use as these can be a contamination source To check the flow sensor calibration Run the Flow Sensor calibration script with the Inspection Only parameter enabled see Calibrate Flow Sensors on page 51 This checks that the flow sensor calibration is working within specifications Field Service Repairs If any of these instrument components is irreparably damaged contact your local Thermo Fisher Scientific field service engineer for repairs e Autosampler or autosampler cooler e Rotary valve e Pump for both instruments or pump PCB for the EASY nLC II instrument e Integrated computer e Touch screen monitor CAUTION Chapter 5 Field Service Maintenance describes how to replace these instrument components However only a Thermo Fisher Scientific field service engineer can perform these procedures Attempted repairs by untrained personnel might cause personal injury or irreparably damage the instrument Thermo Scientifi
237. mum fwhm using PepMap nano bore columns as supplied at delivery Carryover Typically lt 0 05 Conditions Injection of 100 fmol tryptic digests of BSA on Pepmap columns as supplied at delivery and by using the standard wash procedure for the autosampler Autosampler sample pickup 0 1 to 18 pL with the standard 20 uL loop volume range 0 1 to 48 pL with a 50 pL loop Injection reproducibility 0 2 at 5 uL pickup injection RSD 3 0 at 0 1 pL pickup Injection linearity 0 9985 at 0 5 to 10 uL injection volume Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 23 1 Introduction Specifications Table 3 Performance specification Sheet 2 of 2 Item Autosampler formats Specification ASC model 6x 8 Vials 6 Vials 1 x 96 well MTP 6 Vials 1 x 384 well MTP 6 Vials 2x 48 PCR strips 6 Vials 4x24 PCR strips 6 Vials ASA model 6x 8 Vials earlier versions of the 1x96 well MTP EASY nLC II 1 x 384 well MTP instrument 2x 48 PCR strips Autosampler cooling Technical Specifications 4 x 24 PCR strips 20 C below ambient typical setting 7 C 45 F These tables list the technical specifications for the EASY nLC instrument e General specifications Table 4 e Physical specifications Table 5 e Electrical specifications Table 6 e Communication specifications Table 7 e Hardware components able 8 Table 4 General specifications Item Specification So
238. n Augenverletzungen verursachen Tragen Sie beim Umgang mit Chemikalien oder bei der Wartung des Ger tes eine Schutzbrille Allgemeine Gefahr Es besteht eine weitere Gefahr die nicht in den vorstehenden Kategorien beschrieben ist Dieses Symbol wird im Handbuch au erdem dazu verwendet um den Benutzer auf Anweisungen hinzuweisen Wenn Sie sich ber die Sicherheit eines Verfahrens im unklaren sind setzen Sie sich bevor Sie fortfahren mit Ihrer lokalen technischen Unterst tzungsorganisation f r Thermo Fisher Scientific San Jose Produkte in Verbindung ATTENTION Choc lectrique L instrument utilise des tensions capables d infliger des blessures corporelles L instrument doit tre arr t et d branch de la source de courant avant tout intervention Ne pas utiliser l instrument sans son couvercle Ne pas enlever les tuis protecteurs des cartes de circuits imprim s Chimique Des produits chimiques dangereux peuvent se trouver dans l instrument Portez des gants pour manipuler tous produits chimiques toxiques canc rig nes mutag nes ou corrosifs irritants Utiliser des r cipients et des proc dures homologu es pour se d barrasser des d chets d huile Haute Temperature Permettre aux composants chauff s de refroidir avant tout intervention Incendie Agir avec pr caution lors de l utilisation du syst me en pr sence de gaz inflammables Danger pour les yeux Des projections chimiques liquides
239. n earlier versions of the EASY nLC II instrument Figure 4 ASA autosampler model installed in earlier versions of the EASY nLC II instrument zaxis needle holder Adapter plate holder with peltier cooling Wash bottles Xaxis in and out 4 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 1 Introduction Hardware Components Figure 5 shows the tray compartment for the ASA autosampler model as viewed from above when the tray compartment door is open The tray compartment of the ASA autosampler model holds a vial adapter or a well plate adapter three wash solvent bottles and a wash bottle with a wash insert for cleaning the autosampler needle The ASA autosampler model does not hold six additional vials Figure 5 Tray compartment for the ASA autosampler model viewed from above Solvent System Components Behind the Right Side Panel The EASY nLC solvent delivery system is located behind the right side panel and consists of three syringe pumps three pressure sensors two flow sensors four rotary valves four check valves two for each assembly and a mixing Tee Note Two of the syringe pumps pumps A and B produce the binary mobile phase and the third syringe pump pump S draws the sample into the sample loop that is attached to one of the rotary valves valve S These topics describe the hardware components behind the right side panel e View Behind the Right Side Panel e Syri
240. n go to step 10 for an EASY nLC 1000 system or step 11 for an EASY nLC II system To determine if the inline filter connected to the flow sensor inlet of the EASY nLC 1000 system is blocked do the following a Remove the inline filter from the flow sensor inlet b Depending on the back pressure do one of the following e Ifthe pressure is lt 20 bar the inline filter is blocked Replace it with a new inline filter e Ifthe pressure is gt 20 bar reconnect the inline filter to flow sensor A and then go to step 11 EASY nLC Series Troubleshooting and Maintenance Guide 255 6 Troubleshooting Troubleshooting a System Blockage 11 To determine if the line that connects valve A to flow sensor A is blocked do the following a Disconnect the solvent line from port 6 of valve A b Depending on the back pressure do one of the following e Ifthe pressure is lt 20 bar the line from valve A to flow sensor A is blocked Replace it with a new solvent line e Ifthe pressure is gt 20 bar reconnect the line to valve A and then go to step 12 12 To determine if valve A is blocked do the following a Disconnect the solvent line from port 1 of valve A b Depending on the back pressure do one of the following e Ifthe pressure is lt 20 bar valve A is blocked Inspect the valve and clean the stator If the blockage persists replace the rotor seal e Ifthe pressure is gt 20 bar reconnect the line to valve A and then go to ste
241. n of the whole or any part of this document is strictly prohibited except with the written authorization of Thermo Fisher Scientific Inc The contents of this document are subject to change without notice All technical information in this document is for reference purposes only System configurations and specifications in this document supersede all previous information received by the purchaser Thermo Fisher Scientific Inc makes no representations that this document is complete accurate or error free and assumes no responsibility and will not be liable for any errors omissions damage or loss that might result from any use of this document even if the information in the document is followed properly This document is not part of any sales contract between Thermo Fisher Scientific Inc and a purchaser This document shall in no way govern or modify any Terms and Conditions of Sale which Terms and Conditions of Sale shall govern all conflicting information between the two documents Release history Revision A March 2012 Revision B January 2013 Revision B includes information about replacing a PLF pump with a PLU pump information about upgrading the touch screen software and improved troubleshooting procedures Hardware versions EASY nLC II and EASY nLC 1000 instruments Software version Touch screen software version 3 1 For Research Use Only Not for use in diagnostic procedures Regulatory Compliance Thermo Fisher Scientific
242. n seal with methanol 3 Place the pump house on the guide rods and evenly press it all the way down to insert the seal and then gently remove the house when the seal is in position Figure 5 Inserting the piston seal into the pump head 4 Reassemble the pump head on the pump using the two screws and a3 mm hex wrench Do not connect the stainless steel tubing to the pump head 5 Turn on the EASY nLC system and log in as an administrator 6 Fill the pump head with the appropriate solvent as follows a Press Home gt Overview b Press the pump icon for the pump that you want to control The Position readback displays the current pump position from 0 pL empty to 140 pL full c Set the flow rate to 300 pL min and the volume to 140 pL Then press Start The piston moves up to the top of the pump head Figure 6 Pump dialog box Pump A Pressure Bar Pasition 27 75 pl Real flow nl min 328 d Pipette the appropriate solvent into the pump head Figure 7 Adding solvent to the pump head Te at e Set flow rate to 300 pL min and the volume to 140 pL Then press Start The piston moves down drawing solvent into the pump head Make sure the pump head is filled with solvent during the entire retraction of the piston A 7 Using a 1 4 in open ended wrench reconnect the tubing to the pump head see Figure 2 on page 327 CAUTION Be careful not to spill solvent onto the pr
243. n the solvent path from valve A to flow sensor A Go to step 3 e Ifthe script fails the leak is in pump A Go to Troubleshooting a Pump that Fails the Leaks Script on page 216 3 Check the connections to port 6 of valve A and the inlet to flow sensor A as follows e For the EASY nLC II instrument do the following Hand tighten the PEEK fitting connected to the flow sensor inlet Using a 1 4 in open ended wrench tighten the stainless steel fitting connected to port 6 of valve A e For the EASY nLC 1000 instrument return the flow path upstream of valve S to atmospheric pressure as described in the Using nanoViper Fittings Quick Reference Guide on page 323 Then individually disconnect and reconnect the nanoViper fittings connected to port 6 on valve A and the flow sensor A inlet Take care not to overtighten these fingertight fittings 4 Rerun the system leak test Depending on whether the test fails or passes do the following e Ifthe test fails follow the instructions in Maintaining the Rotary Valves on page 74 to fix a leaking valve which in this case is valve A These instructions guide you through the process of cleaning the valve s rotor seal and stator rerunning the system leak test and then replacing the rotor seal if necessary e Ifthe test passes the system is leak tight You have completed this troubleshooting procedure Return the EASY nLC instrument to normal operation Thermo Scientifi
244. nLC system on the Configuration gt Network page The stand alone EASY nLC instrument is not connected to a laboratory LAN port with intranet access Connect the stand alone EASY nLC instrument to a laboratory LAN port with intranet access The LC MS system is not connected to a laboratory LAN port with intranet access EASY nLC Series Troubleshooting and Maintenance Guide Connect the data system computer for the LC MS system to a laboratory LAN port with intranet access Thermo Scientific 6 Troubleshooting Troubleshooting Communication Problems Network Connection Failures Thermo Scientific Table 26 lists the possible causes of a network connection failure Figure 233 shows the Thermo EASY nLC Configuration dialog box that you access from the Thermo Foundation Instrument Configuration application When the EASY nLC instrument cannot establish communication with the data system computer the Connection Failed message appears when you click Test Connection Figure 233 Network connection failure EASY nLC Network Configuration IP Ad ress Mostname 10 32 134 79 About box on the EAST ALC instrument User name admin Run samples as this user User must exist on the EASY nLC Connection failed LC device driver and instrument information LC device driver LCServer 1 1 1 1 Autosampler Configuration Solvents for LC Pump pate teed G a Sample layout Row first Startpos A1 B ace
245. nd Maintenance Guide 237 6 Troubleshooting Troubleshooting the Results of the System Leak Test d On the Home gt Overview page check the readback for flow sensor A Then depending on the flow rate do one of the following e If the flow rate is lt 100 nL min do the following i Stop the Analytical Column Equilibration script and return the system to atmospheric pressure ii Disconnect the damaged solvent line from port 4 of valve S iii Install a new solvent line to connect the mixing Tee outlet to port 4 of valve S For ordering information see Table 33 on page 316 iv Rerun the system leak test see Running a System Leak Test on page 218 e If the flow rate is gt 100 nL min go to the next step 3 To eliminate the solvent B channel as the leak source do the following a Set valve A to the Center position to return the system to atmospheric pressure while maintaining pressure on the front end of the system b Disconnect the blind fitting from the mixing Tee outlet and then reconnect the solvent line to the mixing Tee outlet c Disconnect the solvent line from the mixing Tee inlet for the solvent B channel and then block the inlet with a blind fitting Figure 223 shows the solvent line disconnected from the mixing Tee and a blind fitting in the inlet port of the mixing Tee for the solvent B channel d Reset valve A to position 1 6 238 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Sci
246. nd Maintenance Guide 311 M Consumables and Replacement Parts To order consumables and replacement parts for the EASY nLC system see the ordering information and solvent system schematics in this chapter Contents e Ordering Information e Solvent System Schematic for the EASY nLC II Instrument e Solvent System Schematic for the EASY nLC 1000 Instrument e Common Replacement Parts Ordering Information To obtain ordering information for the EASY nLC instrument s spare parts and accessories go to the following Web sites e For the EASY nLC 1000 instrument go to http www proxeon com productrange nano_IC_easy nlc_1000 accessories_spares e For the EASY nLC II instrument go to http www proxeon com productrange nano_IC accessories_spares These EASY nLC parts are consumable parts and not covered by the normal first year Limited Warranty offered by Thermo Fisher Scientific or any other service contract agreement containing extended warranty coverage e All flow lines including Tee pieces and fittings nuts ferrules sleeves and valve stops e Check valves e Solvent filters inline filters and all associated filter holders e Pump piston seals e Valve rotors and stators e All bottles and lids All sample vials microtiter plates and associated lids and mats e Columns e Fuses e Autosampler needle Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 313 C Consumables and Replacement Part
247. nd locate leaks Because this troubleshooting procedure requires you to handle the solvent lines you must wear gloves and safety glasses CAUTION Wear powder free gloves and safety glasses when handling parts of the LC system that come into contact with solvents Table 23 Materials required for running the system leak test and troubleshooting leaks Instrument Union Plug or blind fitting EASY nLC II P N SC600 Zero dead volume pe union for 1 32 in OD tubing EASY nLC 1000 P N SC900 Thermo Scientific Dionex P N 6040 2303 gt gt EAA iS HPLC zero dead volume union for 1 16 in OD tubing To run a system leak test follow these steps 1 To prepare the instrument for a system leak test on page 219 2 To run a system leak test on page 219 218 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific KD I Thermo Scientific 6 Troubleshooting Running a System Leak Test To prepare the instrument for a system leak test Run the Flush Air script for pump A as follows a Press Maintenance gt Scripts b In the Category list select Prepare In the Name list select Flush Air c Press the Parameters tab d In the Flush Threshold pL box enter the following value e For the PLF model pump EASY nLC II instrument enter 10 e For the PLU model pump EASY nLC 1000 instrument enter 12 Figure 25 on page 33 shows the parameters for the Flush Air script e Press Star
248. nge Pumps e Pressure Sensors e Flow Sensors e Check Valve Assemblies e Six Port Rotary Valves e Autosampler Needle View Behind the Right Side Panel You can access the solvent system components by removing the instrument s right side panel which is secured to the housing with three captive quarter turn screws Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 5 1 Introduction Hardware Components Figure 6 shows the EASY nLC II solvent system Figure 6 Solvent system components behind the right panel of the EASY nLC II instrument Mixing Tee Autosampler needle Waste In line Valv Column Out line Flow sensors A and B 6 EASY nLC Series Troubleshooting and Maintenance Guide Solvent A check valve assembly Solvent B check valve assembly Rotary valves W S A and B Pressure sensors S A and B Pumps S A and B Thermo Scientific 1 Introduction Hardware Components Figure 7 shows the solvent system components for the EASY nLC 1000 instrument Figure 7 Solvent system components behind the right panel of the EASY nLC 1000 instrument
249. nstrument on page 21 c Disconnect the power cable from the instrument s back panel 3 Using a 2 Phillips head screwdriver with a quarter turn loosen the three captive screws that secure the right side panel to the instrument housing Then remove the panel Figure 164 shows the solvent line connections from pump A to valve A which are made with PEEK tubing The solvent line connections from pump B to valve B are made with stainless steel tubing Figure 164 Pump connections in an EASY nLC II instrument PEEK tubing for pump A Valve A Valve A gt E 7O Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 155 5 Field Service Maintenance Replacing a Pump 4 Disconnect the subsystem tubing between the pump head and the high pressure valve as follows Note The PLF pump can have an internally or an externally threaded pump head The latest version of the PLF pump has an internally threaded pump head a Disconnect the tubing from the pump head as follows e Ifa PEEK fitting is connected to the pump head use a 13 mm open ended wrench to remove it see Figure 165 Figure 165 Externally threaded pump head for a PLF model pump PEEK fitting c
250. nt and identifies the location of a leak Before you run a system leak test you must connect the Column Out line to the Waste In line with the HPLC union Figure 35 Pressurized lines for a system leak test Pump S NS Pressure sensor Autosampler needle Pump A w G Pressure EE gr sensor g Orua Flow sensor e E HBR SSE a SSeS EE Ya ae Te Pump B Q m Column Out line iil Pressure Waste In Line f sensor Pa ae Sa Se eee EN Flow sensor g f Centered Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 45 3 Maintenance Scripts and Service Records Maintenance Scripts Torun the Leaks script 1 Open the Leaks script as follows a Press Maintenance gt Scripts b In the Category list select Test c Inthe Name list select Leaks 2 Set up the parameters for this script as follows a Press the Parameters tab b To select pump A or B both pumps or the entire system flow path for the leak test press the cell in the Value column and make your selection from the list e To test the solvent path from pump A to valve A upstream of valve A select A To test the solvent path from pump B to valve B upstream of valve B select B e To test the solvent path from pump A to valve A and from pump B to valve B upstream of pumps A and B select A B e To test the solvent path downstream of valves
251. nt or add next from column a high organic hold to the end of the gradient To determine if the high organic hold is long enough make sure that you see a pressure drop during the hold period see Figure 202 and Figure 203 Insufficient washing of needle Add extra wash steps to the method Consider and loop using an organic solvent for one of the custom wash solvents Dead volumes in the column Remake the connections in the column setup setup Figure 202 shows a gradient run with a high organic hold at the end of the run that is too short for the column to see the high organic solvent as displayed by the lack of a pressure drop at the end of the run Figure 202 Gradient run without an adequate high organic hold at the end of the run Thermo fome Batch Setup Method Editor Maintenance Configuration SCIENTIFIC Overview Pump A Graphs Desired flow Real flow Pressure eq aunssaig F E E Z e Job 00 24 42 Sample 00 24 42 Gradient 14 00 20 Jul 2011 16 15 admin AE 25 50 75 100 125 150 175 Exit Time min 194 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific Thermo Scientific 6 Troubleshooting Troubleshooting Tips Figure 203 shows the effect of a high organic hold at the end of the gradient run on pressure The pressure continues to drop until the column equilibrates to the high organic content Figure 203 Gradient run with an adequate
252. ntenance Guide Thermo Scientific 6 Troubleshooting Using the Direct Controls for Troubleshooting and Maintenance Using the Direct Controls To Fill or Empty Pump S Thermo Scientific Before using the direct controls to fill or empty pump S make sure that the autosampler needle is inserted into the appropriate wash or waste bottle IMPORTANT To avoid contaminating pump S take care to place the autosampler needle in the appropriate wash bottle before filling the pump 1M Kod 1 2 To fill pump S Make sure that a wash bottle with the appropriate solvent is installed in position W3 Send the autosampler needle to the appropriate position as follows a d a On the Home gt Overview page press the autosampler icon The Autosampler dialog box opens see Figure 210 on page 210 Press W3 The autosampler needle moves to the W3 position Press Needle Down The autosampler needle descends into the W3 bottle Press Close to close the Autosampler dialog box Draw solvent into pump S as follows Press the pump S icon The Pump S dialog box opens In the flow rate box enter the filling flow rate 0 01 to 300 pL min For best results enter a flow rate of 40 L min to fill pump S In the volume box enter the volume that you want pump S to draw Press Start The pump draws solvent until it draws the requested volume or reaches the full position whichever occurs first To empty pump S to waste
253. ntrols iit eet ins BELG ae Gok mate 204 Using the Pump Controls 3505 6p eAG ad tay on barns daa eras 205 Using the Autosampler Controls ec cus betaine fall tet eke eis 210 Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide xv Contents Troubleshooting a Pump that Fails the Flush Air Script 212 Troubleshooting a Pump that Fails the Leaks Script 00 216 R nning a System Leak Westie cde ua kien tian Ue aan Vee eels 218 Troubleshooting the Results of the System Leak Test 0 221 Checking Valve Sfora Leik rcsiiacperirs erris estin Be nu pleck Setet 222 Checking the Sample Loop Connections for a Leak 04 222 Locating a Leak in the Solvent Path from Valve B to Flow Sensor B 223 Locating a Leak in the Solvent Path from Pump A to Flow Sensor POP ig a Dec er teen Gitar be ces eae Mari ER ease 224 Locating a Leak In or Between Valve S and Valve W 00000 226 Locating a Leak Between the Flow Sensors and Valve S 234 Identifying a Leaking Check Valve 0 eee cc eee 248 Troubleshooting a System Blockage ihe seis be bees awen see ee kes 249 Running the Back Pressure Test for the A and B Solvent Paths 250 Troubleshooting a System Blockage when Test Solvent A Fails 251 Troubleshooting a System Blockage when Test Solvent B Fails 257 Troubleshooting the Autosampler Aspiration and Calibration 261
254. o continue until you press Stop select the Run Indefinitely check box EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 3 Maintenance Scripts and Service Records Maintenance Scripts 3 Press Start When either pump runs out of solvent both pumps refill The refilling process takes approximately 2 minutes Test MS Connection The MS Connection script tests the contact closure function between the EASY nLC instrument and the mass spectrometer Figure 29 shows the MS connection script Figure 29 MS Connection script Thermo Home Batch SCIENTIFIC Sem oa Maintenance i telaiteele vatelg Log Book f fest connection X Support Devices Tests the connection to the mass spectrometer using gradient ready and f gradient done events No graph Job 00 15 48 11 rey Pu admin Exit To run the MS Connection script and test the contact closure connection 1 Connect the contact closure cable to the EASY nLC instrument and the mass spectrometer Figure 30 shows the contact closure cable for an EASY nLC instrument to be used with a Thermo Scientific mass spectrometer Figure 30 Thermo Fisher contact closure cable Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 39 3 Maintenance Scripts and Service Records Maintenance Scripts Tip Use the Thermo Fisher contact closure cable LC160 to make contact closure between the EASY nLC instrument
255. o one of the following e Ifthe status changes from Not Connected to Connected go to step 3g e Ifthe connection fails see To troubleshoot a failed connection to the support server on page 292 When the connection fails the SSH Connection Error dialog box might appear with this message Not able to determine connection status See message log for details The message log file is in the admin systemLog folder on the EASY nLC system For information about accessing the admin folder from another computer on your local network refer to the EASY nLC Series Getting Started Guide While the message log file might provide useful information to a member of Thermo Scientific Technical Support users should follow the suggested troubleshooting procedure To troubleshoot a failed connection to the support server on page 292 g After the status changes to Connected allow enough time for the EASY nLC system to create the zip file in the admin folder and for the support server to retrieve the zip file Then go to step 4 After you send a message with the appropriate contact information through the remote support feature a member of the Thermo Fisher Scientific Technical Support team will contact you 4 To close the communication link press Disconnect 5 To return the system to normal operation do the following a Reconnect the Ethernet cables as appropriate For information about connecting the EASY nLC system to a da
256. ocedure e The adapter plate holder The adapter plate holder for the ASC model holds an additional six sample vials This difference affects the autosampler calibration procedure and the maximum number of sample vials that you can run without reloading the autosampler ASC Model Current Figure 2 shows the ASC autosampler model that is installed in the current versions of the EASY nLC II and EASY nLC 1000 instruments Figure 2 ASC autosampler model installed in the current versions of the EASY nLC instruments y axis left and right Zaxis needle holder Sample vial tray xaxis in and out Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 3 1 Introduction Hardware Components Figure 3 shows the tray compartment for the ASC autosampler as viewed from above when the tray compartment door is open The tray compartment of the ASC autosampler model holds a vial adapter or a well plate adapter six additional vials three wash solvent bottles and a wash bottle with a wash insert for cleaning the autosampler needle Figure 3 Tray compartment for the ASC autosampler model viewed from above Well plate 96 or 384 Three positions for Waste bottle with wash Six extra vial positions for or vial 6 x 8 adapter wash solvents insert for cleaning of the standards or regular outside of the needle samples ASA Model Legacy EASY nLC II Instruments Figure 4 shows the ASA autosampler model that is installed i
257. ocument by clicking the button below SURVEY If you are reading a printed copy of this manual complete a brief survey about this document by going to the following URL http www surveymonkey com s PQM6P62 Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide xix Preface Related Documentation In addition to this guide Thermo Fisher Scientific provides the following documents for the EASY nLC instrument EASY nLC Series User Guide for the Xcalibur 2 x Data System EASY nLC Preinstallation Requirements Guide EASY nLC Series Getting Started Guide Downloading Manuals from the Customer Manuals Web Site For Thermo Scientific software and hardware products Thermo Fisher Scientific provides instructional guides as PDF files on its Customer Manuals Web site Note Ifyou are using a Thermo Scientific application to control the EASY nLC system as part of an LC MS system the EASY nLC manuals are also available from the data system computer s Start menu iM Xa To download the EASY nLC manuals from the Customer Manuals Web site 1 Go to mssupport thermo com 2 On the Terms and Conditions page click I Agree 3 Click Customer Manuals in the left margin of the window 4 On the right side of the window click Search The Documents Advanced Search page opens Thermo SCIENTIFIC GS ets Tug Thermo Fisher Scientific Home gt gt Customer Dovnload Documents Advanced Search Ad
258. odel do the following e For the EASY nLC II instrument go to step 3 to remove the front panel e For the EASY nLC 1000 instrument go to step 4 to remove the top front panel 3 To remove the front panel from the EASY nLC II instrument do the following a Using a flat edged tool pry off the Thermo blue applique b Using a Torx T 10 wrench remove the 10 screws that secure the front plate to the EASY nLC II instrument Then remove the plate see Figure 103 c Goto step 5 Figure 103 Removing the front plate from the EASY nLC II instrument Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 113 5 Field Service Maintenance Replacing the Monitor 4 To remove the top front panel from the EASY nLC 1000 instrument do the following a Using a Torx T 10 wrench remove the two screws that secure the top front panel to the top back of the instrument b Open the tray compartment door and then use a Torx T 10 wrench to remove the screw that secures the top front panel to the front of the instrument 5 Using a 2 mm hex wrench remove the four screws that secure the monitor to the instrument see Figure 104 CAUTION Use only hand tools with a hex tip to avoid damaging the screw heads Figure 104 Removing the screws from the front of the monitor 6 Using a 2 Phillips head screwdriver with a quarter turn loosen the three captive screws that secure the left side panel to the instrument housing Then
259. om the slot in the panel Black needle Slot in the holder metal plate Holder F i _ 5 Connect the needle to port 1 of Valve S as follows a Slide the provided blue sleeve and metal fittings onto the tubing b To ensure a zero dead volume connection hold the blue sleeve and the tubing firmly against the bottom of the valve port and then tighten the fitting with a 1 4 in open ended wrench Recalibrate the needle position 6 e For the ASA model remove the white plastic needle guide on 7 Press Maintenance gt Scripts the back of the touch screen monitor 8 In the Category list select Prepare 9 Loosen the nut that is connected to the z axis needle holder by 9 In the Name list select Purge Solvent Then run 2 iterations of turning it counterclockwise Then carefully pull the autosampler the Purge Solvent script for Pump S needle upward and away from the holder 10 In the Name list select Flush Air Then run the Flush Air script for Pump S with a flush volume threshold of 10 pL for the EASY nLC II system or 12 pL for the EASY nLC 1000 system 334 S Index A Acquisition Server errors 189 adapter plate replacing ASA autosampler 272 ASC autosampler 271 air removing from pump heads 33 Analytical Column Equilibration script 36 application version 20 ASA autosampler adapter plate replacing 272 description 4 needle description of 14 See also autosampler upgrading to ASC autosampler service
260. onnected to an externally threaded pump head e Ifa stainless steel fitting is connected to the pump head see Figure 166 use a 1 4 in open ended wrench to remove it Figure 166 Internally threaded pump head for a PLF model pump g Stainless steel fitting connected to an internally threaded pump head 156 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 5 Field Service Maintenance Replacing a Pump b While holding the pressure sensor use a 1 4 in open ended wrench to disconnect the tubing connected to the pressure sensor s inlet and outlet ports see Figure 167 Figure 167 Disconnecting the solvent lines from the pressure sensor Solvent lines connected to the pressure sensor c Using a 1 4 in open ended wrench disconnect the other end of the tubing that is connected to the subsystem valve as follows e For valve S disconnect the tubing from port 6 e For valve A or B disconnect the tubing from port 1 5 Disconnect the pressure sensor assembly and the damaged PLF pump from the instrument panel as follows a Using a 2 5 mm L hex wrench or ball driver remove the two hex screws that secure the pressure sensor L bracket to the instrument panel see Figure 168 Then set the two 2 5 mm hex screws aside for reuse when you reconnect the L bracket to the instrument panel b Using a2 mm L hex wrench or ball driver unscrew the two hex screws that secure the pressure sensor to the L
261. or for a PLF Pump e Replacing the ASC Autosampler e Upgrading from an ASA Autosampler to an ASC Autosampler e Replacing the Autosampler Cooler e Replacing a Rotary Valve e Replacing a Pump e Replacing a Pump PCB Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 107 5 Field Service Maintenance Replacing the Built In Computer Replacing the Built In Computer The procedure for replacing the built in computer is the same for the EASY nLC 1000 and EASY nLC II instruments except where noted Replacing the computer box requires the following part and tools Tools Parts e 2 Phillips screwdriver Computer box e Torx T 10 wrench e EASY nLC II instrument P N LC285 e Small flathead screwdriver a e EASY nLC 1000 instrument P N LC585 To replace the built in computer follow these steps 1 To remove the computer box on this page 2 To install the new computer box on page 112 To remove the computer box 1 Close down the EASY nLC instrument see Closing Down the EASY nLC Instrument on page 21 and then unplug the power cable 2 Disconnect all of the cables from the instrument s back panel 3 Using a 2 Phillips head screwdriver with a quarter turn loosen the three captive screws that secure the left side panel to the instrument housing Then remove the panel 4 Remove the internal cables connected to the computer box Note The cable connectors can be hard to loosen You m
262. or seal see Replacing the Rotor Seal on page 77 5 If the problem persists after replacing the rotor seal replace the sample loop see Replacing the Sample Loop on page 89 222 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 6 Troubleshooting Troubleshooting the Results of the System Leak Test Locating a Leak in the Solvent Path from Valve B to Flow Sensor B Thermo Scientific The system leak test returned the following message see Table 24 on page 221 Check the B solvent line valve B to flow sensor B and valve B for leaks Flow sensor B has detected solvent flow toward valve B This negative flow rate is caused by a leak in valve B or in the connections to port 6 of valve B and the flow sensor inlet Note When the system is leak tight and valve B is in the Center position the solvent flow through flow sensor B from pump A should be negligible A negative flow rate of up to 30 nL min is within the normal range A higher negative flow rate indicates a leak that could affect the retention time of analytes Figure 213 shows a reading for flow sensor B of 60 nL min This means there is a leak in the solvent path from valve B to flow sensor B Figure 213 Solvent line between valve B and flow sensor B Possible leaking connections Valve B 60 nL min Flow sensor B lt To troubleshoot a leak in the solvent line connections between valve B and flow sensor B
263. ork must allow outgoing TCP IP traffic from the EASY nLC system to the support server at IP address 195 41 108 93 port 22 When you establish the communication link with the support server the support server triggers the file compression utility on the EASY nLC instrument to build a zip file with the appropriate log files and then retrieves the zip file from the EASY nLC instrument Meanwhile the EASY nLC instrument sends an e mail with your supplied message and contact information to Thermo Fisher Scientific Technical Support After you send a message through the remote support feature a member of the Thermo Fisher Scientific Technical Support team will contact you Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 287 8 Remote Support Connecting the EASY nLC Instrument to the Support Server Connecting the EASY nLC Instrument to the Support Server Use this procedure to establish a communication link between the EASY nLC instrument s computer and the support server R To open a communication link between the EASY nLC system and the support server 1 Using a shielded Ethernet cable connect the LAN port on the back panel of the EASY nLC system see Figure 250 directly to a laboratory LAN port that provides access to the Internet To use the remote support feature the firewalls for your local network must allow outgoing TCP IP traffic from the EASY nLC system to the support server at IP address 195
264. osition Directs the solvent flow toward the column by blocking the solvent path through valve W 1 6 position Directs the solvent flow from the venting Tee to the waste beaker Centered Blocks the solvent flow through the valve to the waste beaker EASY nLC Series Troubleshooting and Maintenance Guide 13 1 Introduction Hardware Components Autosampler Needle During a sample run the autosampler needle descends into the requested sample vial or well and then pump S draws the requested sample volume into the needle tubing The autosampler needle is a user replaceable part For information about replacing the autosampler needle see Replacing the Autosampler Needle on page 84 The autosampler needle for the ASC autosampler is a length of PEEKsil tubing with a sleeve and a stainless steel fitting at one end and a PEEK nut and plastic stop at the other end see Figure 13 Fitting into a slot in the panel between the solvent system compartment and the tray compartment the needle retainer properly positions the needle tubing Figure 13 Autosampler needle for the ASC autosampler current EASY nLC II and EASY nLC 1000 instruments Stainless steel fitting a and sleeve Needle PEEK nut retainer Plastic stop Figure 14 shows autosampler needle for the ASA autosampler Figure 14 Autosampler needle for the ASA autosampler model earlier EASY nLC II instruments For both needles the stainless steel fit
265. oting and Maintenance Guide 243 6 Troubleshooting Troubleshooting the Results of the System Leak Test To locate a leak between flow sensor B and the mixing Tee in the EASY nLC 1000 1 To ensure leak tight connections for the solvent line that connects the outlet of flow sensor B to the mixing Tee inlet do the following a Set valve A to the Center position b Set up the system plumbing as follows i Remove the blind fitting from the mixing Tee s inlet for the solvent B channel ii Reconnect the solvent line to the solvent B channel inlet of the mixing Tee iii Redo the nanoViper connection to the mixing Tee s inlet for the solvent B channel The system plumbing now matches that for a system leak test c Reset valve A to position 1 6 d Check the readback for flow sensor A Then depending on the flow rate do one of the following e Ifthe flow rate is lt 100 nL min you have completed this troubleshooting procedure Return the instrument to normal operation e If the flow rate is gt 100 nL min go to the next step 2 Using a 5 16 in open ended wrench tighten the inline filter to the outlet of flow sensor B 3 To determine whether the solvent line or the inline filter is the leak source do the following a Set valve A to the Center position b Disconnect the solvent line from the outlet of flow sensor B Then plug the free end of the solvent line with a leak test union and a blind fitting see Figure 2
266. ottom right positions on the plate formats Plate format Top left position Bottom right position 6x8 Al F8 8x12 Al H12 16x24 Al P24 To calibrate plates 1 If you have not already done so prepare the autosampler for the plate calibration routine see To prepare the autosampler for the plate calibration routine on page 278 2 Open the Autosampler Plate Calibration dialog box as follows a Press Maintenance gt Devices b In the Devices list select the autosampler c Press the Tools tab The Tools view opens see Figure 244 Figure 244 Tools view for the autosampler About Properties Tools Cooled Autosampler ASC Autosampler Plate Vials Wash f New f Calibrate Current Vials Calibrate la Manipulator 6x8 Vials J L Reset copy to f f 280 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 7 Calibrating the Autosampler s XYZ Robot Calibrating Plates d In the Plate area of the Tools view for the autosampler press Calibrate see Figure 244 on page 280 The Autosampler Plate Calibration dialog box opens Figure 245 shows the dialog box at the beginning of the plate calibration routine The top left and bottom right positions are highlighted in green The position readbacks for the xyz axes are unpopulated Figure 245 Plate view in the Autosampler Plate Calibration dialog box Autosampler Plate Calibration Calibrate Plate
267. ou solides peuvent tre dangereuses pour les yeux Porter des lunettes de protection lors de toute manipulation de produit chimique ou pour toute intervention sur l instrument Danger g n ral Indique la pr sence d un risque n appartenant pas aux cat gories cit es plus haut Ce symbole figure galement sur l instrument pour renvoyer l utilisateur aux instructions du pr sent manuel Si la s ret d une proc dure est incertaine avant de continuer contacter le plus proche Service Client le pour les produits de Thermo Fisher Scientific San Jose PRECAUCION Descarga el ctrica Este instrumento utiliza altas tensiones capaces de producir lesiones personales Antes de dar servicio de mantenimiento al instrumento ste debera apagarse y desconectarse de la l nea de alimentacion el ctrica No opere el instrumento sin sus cubiertas exteriores quitadas No remueva las cubiertas protectoras de las tarjetas de circuito impreso Qu mica El instrumento puede contener productos quimicos peligrosos Utilice guantes al manejar productos quimicos t xicos carcinogenos mutagenos 0 corrosivos irritantes Utilice recipientes y procedimientos aprobados para deshacerse del aceite usado Altas temperaturas Permita que los componentes se enfrien ante de efectuar servicio de mantenimiento Fuego Tenga cuidado al operar el sistema en presencia de gases inflamables Peligro par los ojos Las salicaduras de productos qu
268. ow the workflow in Figure 226 or the procedure on pages 244 245 to locate and remedy the leak Figure 226 EASY nLC 1000 workflow for locating a leak between flow sensor B and the mixing Tee You determined that the leak is in the solvent path between the outlet of flow sensor B and the inlet of the mixing Tee Remove the blind fitting from the solvent B channel inlet of the mixing Tee Reconnect the solvent line to the solvent B channel inlet of the mixing Tee Redo the nanoViper connection to the outlet of flow sensor B step 1 on page 244 The system plumbing now matches that for a system leak test see Figure 35 on page 45 No Readback for flow sensor A gt 100 nL min Done Using a 5 16 in open ended wrench tighten the inline filter to the outlet of flow sensor B step 2 on page 244 No Readback for flow sensor A gt 100 nL min Disconnect the solvent line from the outlet of flow sensor B Then plug the free end of the solvent line with a leak test union and a blind fitting step 3 on page 244 Readback for flow sensor A gt 100 nL min Done Install a new inline filter page 81 No Reconnect all of the solvent lines Then rerun the system leak test page 218 Install a new solvent line between the flow sensor B outlet and the mixing Tee inlet step 3d on page 245 Rerun the system leak test page 218 Thermo Scientific EASY nLC Series Troublesho
269. p 13 13 To determine if the line from pressure sensor A to valve A is blocked or if pressure sensor A is blocked do the following a Disconnect the solvent line from the outlet of pressure sensor A b Depending on the back pressure do one of the following e Ifthe pressure is lt 20 bar the line that connects pressure sensor A to valve A is blocked Replace it with a new solvent line e Ifthe pressure is gt 20 bar pressure sensor A is blocked Replace it with a new pressure sensor as described in Replacing a Pressure Sensor for the PLU Pump on page 90 256 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 6 Troubleshooting Troubleshooting a System Blockage Troubleshooting a System Blockage when Test Solvent B Fails Follow this procedure when the Back Pressure test for Test Solvent B fails see To run the back pressure script for both the A and B solvent paths on page 250 Figure 230 shows the areas that this procedure systematically checks for blockage Figure 230 Solvent system from pressure sensor B to the mixing Tee Mixing Tee Flow sensor outlet to the mixing Tee Inline filter for the EASY nLC 1000 instrument Flow sensor Inline filter for the EASY nLC 1000 instrument From valve B port 6 to the flow sensor inlet Valve B Valve B port 1 to the pressure sensor or the pressure sensor Thermo Scientific EASY nLC Series Troubleshooting and Mainten
270. p File Name Sample ID Path Inst Meth Proc Meth Position Inj Vol Status Acquisition Queue Unknown BlankUDGI A2 C Xcalibur D C Xcalibur m L 200 Run Manager Unknown BlankUDG2 A3 C Xcalibur D C Xcalibur m 10 0 Ready To Download Unknown BlankUDG3 A4 C Xcalibur D C Xcalibur m 10 0 Unknown A5 C Xcalibur D C Xcalibur m 10 0 0 0 Acquisition Server Proxeon_EASY nLC device reported a failure during Prepare For Run command That i hermo EASY nLC The has een paused Ready to Download To resume the list go to the Actions main menu LTQ Velos MS and uncheck the Pause Analysis menu item Ready to Download Ce Figure 199 shows the Acquisition Server message that appears when you incorrectly use a lowercase letter to define the vial or well position For the EASY nLC device you must use capital letters in the alphanumeric description of the vial or well position Figure 199 Incorrect vial position nomenclature Incorrect use of a lowercase letter 5 velos 10 32 134 20 Remote Desktop alka a aan Xcalibur ir Sequence Setup zh 1104141 32511 Open ample Typ File Name Sample ID Path Inst Meth Proc Meth Position Inj Vol Unknown BlankUDG1 A2 C Xcalibur D C Xcaliburm Pil 10 0 Unknown BlankUDG2 A3 C Xcalibur D C Xcalibur m 10 0 Unknown BlankUDG3 A4 C Xcalibur D C Xcalibur m 10 0 Unknown 45 C Xcalibur D C Xcalibur m 10 0 00 Acquisition Server Proxeon_EASY nLC device reporte
271. performs complete testing and evaluation of its products to ensure full compliance with applicable domestic and international regulations When the instrument is delivered to you it meets all pertinent electromagnetic compatibility EMC and safety standards as described in the Declaration of Conformity Changes that you make to the instrument might void compliance with one or more of these EMC and safety standards Changes to the instrument include replacing a part or adding components options or peripherals not specifically authorized and qualified by Thermo Fisher Scientific To ensure continued compliance with EMC and safety standards replacement parts and additional components options and peripherals must be ordered from Thermo Fisher Scientific or one of its authorized representatives FCC Compliance Statement THIS DEVICE COMPLIES WITH PART 15 OF THE FCC RULES OPERATION IS SUBJECT TO THE FOLLOWING TWO CONDITIONS 1 THIS DEVICE MAY NOT CAUSE HARMFUL INTERFERENCE AND 2 THIS DEVICE MUST ACCEPT ANY INTERFERENCE RECEIVED INCLUDING INTERFERENCE THAT MAY CAUSE UNDESIRED OPERATION CAUTION Read and understand the various precautionary notes signs and symbols contained inside this manual pertaining to the safe use and operation of this product before using the device Notice on Lifting and Handling of Thermo Scientific Instruments For your safety and in compliance with international regulations the physical handling of
272. phs Desired flow Real flow Pressure _ Desired flow Real flow ease 10 000 0 300 0 1 000 0 300 0 Queue 7 500 0 Me 750 0 malig 250 0 250 0 5 000 0 4 500 0 250 0 0 0 250 0 500 0 750 0 t 200 0 7 200 0 Flow nl min eq aunssaig Flow nl min ega eunssaug v gt on 1 000 0 s 0 pee LU ob or ere J 5 10 I5 20 2 Time min Time min Pesan oD S F w 100 0 Desired flow Pressure t 90 0 80 0 70 0 60 0 Gradiem 16 44 50 0 19 Jul 2011 es ui 12 11 thee 20 0 admin 4 10 0 f bod 5 10 CT ae Ce A Exit Time min Time min Jod 00 51 48 Sample 00 27 14 Flow nl min 3 aunixiw gy Flow nl min eq aunssaug a o 180 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 6 Troubleshooting Troubleshooting Tips When only the flow path between pump B and the mixing Tee has a blockage the back pressure on pump B increases and differs from the back pressure on pump A Figure 188 shows the typical profile for a blockage in the solvent system between pump B and the mixing Tee Figure 188 Back pressure shown reaching the maximum pressure for pump B only Maximum pressure reached for pump B SCIENTIFIC aa etup Method Editor Mainter on Craps Desired flow Real flow Pressure Desired flow Real flow Pregsure 5 300 0 300 0 300 0 3000 Queue
273. place underneath the mixing Tee holder 11 Mount the aluminum needle arm under the mixing Tee holder see Figure 137 Figure 137 Aluminum needle arm Mixing Tee holder Needle arm 12 Place the mixing Tee in the holder Use the aluminum needle arm when you attach the needle see Figure 138 Figure 138 Needle Mixing Tee Needle arm Mixing Tee holder 13 Reinstall the monitor as described in To install the new monitor on page 116 Tip Do not reconnect the left side panel to the instrument housing until after you recalibrate the autosampler 14 Turn on the instrument and then log in to the instrument as an administrator Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 135 5 Field Service Maintenance Upgrading from an ASA Autosampler to an ASC Autosampler 15 Add the ASC autosampler to the Devices list on the Maintenance gt Devices page as follows a Press Maintenance gt Devices The Maintenance gt Devices page opens b Press Add Devices The Select the Device to Add dialog box opens c Select the ASC autosampler from the list and then press Accept d Verify that the ASC autosampler has been added to the Devices list For more information about adding devices to the Devices list see Managing the Devices List on page 105 16 Calibrate the autosampler as described in Chapter 7 Calibrating the Autosampler s XYZ Robot 136 EASY nLC Series Troubl
274. pler needle connected to port 1 of PEEKsil 150 pm ID 550 mm length LC302 valve S 7 Column Out tubing connected to port 3 of nanoViper 20 pm ID 550 mm length LC560 valve S 8 Waste In line venting Tee to port 2 of nanoViper 75 um ID 550 mm length LC562 valve W 9 Sample loop 20 pL nanoViper 250 pm ID 410 mm length LC472 D Port 2 of valve A to check valve A Teflon 500 pm ID 150 mm length kit LC230 Port 2 of valve B to check valve B E Tubing 2 from check valves to solvent Teflon 500 pm ID 390 mm length kit LC230 bottles F Tubing 2 from check valves to waste beaker Teflon 500 pm ID 390 mm length kit LC230 G Tubing from valve W to waste beaker Teflon 500 pm ID 330 mm length LC263 316 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific C Consumables and Replacement Parts Solvent System Schematic for the EASY nLC 1000 Instrument Figure 260 Solvent system schematic for the EASY nLC 1000 system 00000000 00000000 00000000 00000000 a 00000000 00000000 000000 t Solvent A Solvent B ia Pressure sensor Pressure sensor Waste EE Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 317 C Consumables and Replacement Parts Common Replacement Parts Common Replacement Parts For proper maintenance of your EASY nLC instrument Thermo Fisher Scientific recommends that you maintain an inventory of replacement parts e Common Replac
275. pplication contains five menu tabs at the top of the touch screen When you press a tab at the top of the touch screen a set of side tabs appears on the left side of the touch screen Figure 19 shows the menu layout for the Home gt Overview page Figure 19 EASY nLC touch screen with the menu layout on the Home gt Overview page Top menu tabs Logo icon Batch Setup Method Editor Side tabs Bueniew LSP disabled ee et di Idle flow disabled 0 3 bar 0 0 nl min 140 0 140 0 ul Solvent A 8 9 bar 0 0 nl min 140 0 140 0 pl 3 8 nl min Solvent B Job 00 00 00 11 May 2011 Current user 10 58 Job Current at J Login and Exit button Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 19 1 Introduction Touch Screen Application During a session the application tracks which page side tab you chose inside each menu top tab and shows that page when you next choose that menu The Thermo Scientific logo icon in the upper left corner and the Login button in the lower left corner are always available To view the version information for the application press the Logo icon Logging In to the EASY nLC Instrument for Maintenance Tasks To run the maintenance scripts use the direct controls set up the system configuration and so on you must log in to the EASY nLC instrument as an administrator To log in as a user with administrative privileges 1 Pr
276. pulator 8x12 Abgene AB 0800 5 May 2011 Reset ne fa Exit d Inthe Manipulator area press Reset The z axis needle holder moves to the W1 position 7 Using a 2 Phillips head screwdriver with a quarter turn loosen the three captive screws that secure the right side panel to the instrument housing Then remove the panel 86 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 4 Routine Maintenance Replacing the Autosampler Needle 8 Depending on the instrument model do the following e For the ASC model remove the black needle holder from the slot in the metal plate that separates the solvent system hardware from the autosampler compartment Removing the fitting requires some gentle movement from both sides of the plate see Figure 77 Figure 77 Removing the black needle holder from the slot in the metal plate Black needle holder Slot in the metal plate ff e For the ASA model remove the white plastic needle guide on the back of the touch screen monitor 9 Loosen the nut that is connected to the z axis needle holder by turning it counterclockwise see Figure 78 Then carefully pull the autosampler needle upward and away from the holder Figure 78 Autosampler needle connected to the zaxis needle holder ASA model z axis needle holder ASC model z axis needle holder Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 87 4 Routine M
277. pump S position as follows a Using a3 mm ball driver or L hex wrench and the four 3 mm hex screws that you set aside when you removed the damaged pump from the instrument panel secure the new pump to the pump adapter plate b Connect the pressure sensor cable from the new pressure sensor to the receptacle on the right side of the PLU pump c Connect the P bus communication cables 8 To complete the pump installation start from step 9 on page 159 of the previous procedure To replace a PLF pump with a PLU pump in the EASY nLC II instrument 162 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 5 Field Service Maintenance Replacing a Pump PCB Replacing a Pump PCB The procedure for replacing the pump PCB differs between the PLU model pump and the PLF model pump Note The PLU pump is standard in the EASY nLC 1000 instrument The PLF pump is standard in most EASY nLC II instruments Figure 8 on page 9 shows these two pump models To replace a pump PCB follow the appropriate procedure e Replacing a Pump PCB in the PLU Pump on this page e Replacing a Pump PCB in the PLF Pump on page 167 Replacing a Pump PCB in the PLU Pump Replacing the pump PCB on the PLU model pump requires these items Tools Parts e 2 Phillips screwdriver Pump PCB P N LC574 e 2 5 mm hex wrench e Small flathead screwdriver To replace the pump PCB on the PLU pump follow these steps 1 To remove
278. quires more firmware files reconnect to the server and install the upgrade files as described in step 1 through step 4 of this procedure Table 29 lists the software versions that you must install to upgrade the software to the current version Table 29 Software installation matrix Current version Install versions 2 4 2923 2 5 3032 2 7 8 1 2 8 11 1 and 3 1 4 1 2 5 3032 2 7 8 1 2 8 11 1 and 3 1 4 1 2 7 8 1 2 8 11 1 and 3 1 4 1 2 8 11 1 3 1 4 1 3 1 4 1 current as of January 2013 EASY nLC Series Troubleshooting and Maintenance Guide 297 ee 6 Returning the EASY nLC Instrument If it is necessary to return the EASY nLC system to the factory follow the instructions in this chapter Contents e Transport Instructions e Declaration of Contamination Transport Instructions To prepare the instrument for transport 1 Press Home gt Overview Press Eject Plate Remove the plates tubes Press Insert Plate 2 2 4 Remove the waste beaker and the wash waste bottles 5 6 Close down the system see Closing Down the EASY nLC Instrument on page 21 7 Remove the cables on the back side Tip For the original ASA autosampler only Secure the autosampler by using the transport collars see Figure 258 before shipping the instrument This is not necessary on the latest ASC model Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 299 9 Returning t
279. r remove the two 2 5 mm hex screws at the bottom of the cooler shown in Figure 140 Figure 140 Screws at the bottom of the cooler 5 Using a 2 5 mm ball driver remove the 2 5 mm hex screw in the top center position of the cooler as shown in Figure 141 The screws do not fall out when loosened because they are held in place by O rings Figure 141 Removing the top center screw 138 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific Thermo Scientific 6 Nn ioe 5 Field Service Maintenance Replacing the Autosampler Cooler Pull out the cooler as shown in Figure 142 Figure 142 Pulling out the cooler To install the new ASC cooler Insert the new cooler Be careful not to damage any of the cables during reassembly Figure 143 shows a damaged cable Damaged cable Using a 2 5 mm ball driver insert and tighten the 2 5 mm hex screw in the top center position see Figure 151 on page 143 Using a 2 5 mm ball driver insert and tighten the two 2 5 mm hex screws at the bottom of the cooler see Figure 150 on page 143 Plug the P Bus communication cables into the PCB on the back of the cooler accessing the PCB through the back of the instrument Align the back panel to the back of the instrument Using a Torx T 10 wrench tighten the eleven screws that secure the back panel to the instrument Reconnect the power cable Turn on the EASY nLC instrument
280. r Scientific field service engineer Figure 228 Solvent lines that connect the flow sensor outlets to the mixing Tee and the solvent line that connects the mixing Tee outlet to port 4 of valve S in the EASY nLC II instrument Solvent line that connects the outlet of flow sensor A to the mixing Tee Mixing Tee with fingertight connections Solvent line that connects the outlet of flow sensor B to the mixing Tee Column Out and Waste In lines connected to the leak test union Solvent line that connects the mixing Tee outlet to port 4 of valve S Flow sensor outlets with fingertight connections Solvent lines that connect valves A and B to the flow sensor inlets RQ Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 247 6 Troubleshooting Identifying a Leaking Check Valve Identifying a Leaking Check Valve The EASY nLC instrument has four check valves Each solvent inlet line connects to a check valve assembly with a solvent side check valve and a waste side check valve CAUTION Wear powder free gloves and safety glasses when handling parts of the LC system that come into contact with solvents To determine if the waste side check valve is leaking 1 Run the Purge Solvent script as follows a Press Maintenance gt Scripts b In the Category list select Prepare c Inthe Name list select Purge Solvent d Press
281. readed pump head e Ifa stainless steel fitting is connected to the pump head use a 1 4 in open ended wrench to remove it Figure 2 Stainless steel fitting on the internally threaded pump head pN Stainless steel fitting connected to an internally threaded pump head 5 Using a 13 mm open ended wrench remove the pump head Figure 3 Removing the pump head Pump head 6 Place the piston in the fully extended position as follows a Turn on the instrument and log in as an administrator b Press Home gt Overview and then press the pump icon for the pump you want to control The Pump dialog box opens c Set the flow rate to 300 pL min and the volume to 140 pL d Press Start 7 Clean the piston with the lint free tissue soaked in methanol and visually inspect the piston for any scratches A 8 Return the piston to the fully retracted position as follows CAUTION Take care to avoid solvent drips onto the pump PCB a In the Pump dialog box set the flow rate to 300 pL min and the dispense volume to 140 pL b Press Start 9 When the piston is fully retracted close down the EASY nLC system and turn off the power Removing the Worn Piston Seal 1 Clean the piston seal tool in a beaker filled with 100 methanol Figure 4 Two component piston seal tool for the PLF model pump 2 Insert the piston seal tool into the worn piston seal and pull the piston seal out of the pump head Figure 5
282. rench or ball driver remove the four 2 5 mm hex screws that secure the PCB to the pump Figure 109 shows these screws The lower right screw also connects the pressure sensor grounding cable to the PCB pi ELN Pressure sensor grounding cable 118 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 5 Field Service Maintenance Replacing a Pressure Sensor for a PLF Pump 6 Gently pull the PCB toward you and rotate it to gain access to its back side see Figure 110 Figure 110 Turning the PCB around PLF pump Pressure sensor communication cable connection to the back side of the PCB 7 Disconnect the pressure sensor communication cable being careful not to put any stress on the cables see Figure 111 Figure 111 Disconnecting the pressure sensor communication cable PLF pump Pressure sensor communication cable connection to the back side of the PCB Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 119 5 Field Service Maintenance Replacing a Pressure Sensor for a PLF Pump 8 While holding the pressure sensor use a 1 4 in open ended wrench to disconnect the solvent lines connected to the inlet and outlet of the pressure sensor see Figure 112 Figure 112 Disconnecting the solvent lines from the pressure sensor Solvent lines connected to the pressure sensor 9 Using a 2 5 mm L hex wrench or ball driver remove the two hex screws that secure the press
283. repair 128 ASA cooler replacing service repair 140 ASC autosampler adapter plate replacing 271 description 3 needle description of 14 replacing service repair 122 See also autosampler ASC cooler replacing service repair 137 autosampler calibrating XYZ robot 269 286 checking sample pickup schedule 28 description 2 direct control 210 needle blocked 262 description 14 replacing 84 88 Sample Pickup script 263 Torque script 49 tray compartment accessing 2 troubleshooting tips 171 Thermo Scientific back panel instrument 16 back pressure testing 48 troubleshooting maximum pressure spikes during gradient 180 system blockage 249 too high during sample loading and column equilibration 178 Back Pressure script 48 backup ring 8 beaker waste 2 blank injections troubleshooting 183 184 263 blind fitting EASY nLC 1000 instrument 218 blockage troubleshooting 249 C cables internal connected to EASY nLC 1000 pump 152 cooler bus cable 123 LVDS cable 108 114 116 monitor cable 114 P Bus communication cables 118 158 PC box cable connections 108 pressure sensor communication cable 119 USB cable 114 calibrating autosampler XYZ robot 269 286 flow sensors 51 plates 280 pressure sensors 52 carryover specification 23 troubleshooting tips 194 check valves description 11 replacing 80 EASY nLC Series Troubleshooting and Maintenance Guide 335 Index D checking check valves for leaks 29 248 sample pickup 263
284. rews aside 152 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 5 Field Service Maintenance Replacing a Pump To install a PLU pump in an EASY nLC instrument 1 Connect the new pump as follows a Using a3 mm ball driver or L hex wrench and the four 3 mm hex screws that you removed in step 7 on page 152 secure the new pump to the instrument panel b Depending on the instrument model do one of the following e For the EASY nLC 1000 instrument reconnect the flow sensor cable the pressure sensor cable and the P bus communication cables e For the EASY nLC II instrument reconnect the pressure sensor cable and the P bus communication cables 2 Make sure that the address setting on the pump PCB is the same as the setting for the old PCB Use a small flathead screwdriver to change the rotary switch address on the new pump PCB e Pump A 1 ID e Pump B 2 9 0 7 09 Yr e Pump S 3 rc ww Ig Reconnect the power cable to the instrument s back panel A OG Turn on the EASY nLC instrument and log in as an administrator Nn Prime the pump as described in Priming the Pump on page 71 6 Reconnect the tubing to the pump head Using a 1 4 in open ended wrench tighten the fitting 7 Draw fresh solvent into the solvent lines and remove air from the pump head as described in Removing Air After Replacing a Piston Seal or a Pump on page 73 8 Depending on the pump you are rep
285. ries Troubleshooting and Maintenance Guide Deflect the air from the vent or use a shield to protect the ion source Thermo Scientific Chromatographic Performance 6 Troubleshooting Troubleshooting Tips Table 20 lists troubleshooting tips for common chromatography problems Table 20 Troubleshooting tips for chromatographic performance Symptom Possible cause Broad peaks Dead volume between the column and emitter Action Check the connections Replace the fittings if needed Damaged column Inspect the column especially the outlet Replace the column if it is damaged Poor retention time reproducibility Temperature fluctuations in the laboratory Monitor the laboratory temperature Stabilize the temperature by keeping the laboratory doors closed and by ensuring that the air conditioning system is kept constant at all times Do not run the EASY nLC instrument with the panels removed Back flow on flow sensor B Run the Leaks script for Pump A Pump B and System Replace defective piston seals rotor seals and solvent lines as indicated Spikes seen in flow sensor B during valve shifts Use the scripts to check for a leak in the B subsystem Leak test Pumps A B and System Replace defective seals rotors and solvent lines as needed Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 197 6 Troubleshooting Troubleshooting Tips Device Failures Tab
286. rmine if valve B is blocked do the following a Remove the solvent line from port 1 of valve B and then monitor the pressure b Depending on the pressure do one of the following e Ifthe pressure is below the threshold valve B is blocked Go to Maintaining the Rotary Valves on page 74 Instrument Pressure threshold EASY nLC II 20 bar EASY nLC 1000 10 bar e Ifthe pressure is above the threshold keep the pump running and go to step 8 8 To determine if either the line that connects valve B to pressure sensor B is blocked or pressure sensor B is blocked do the following a Remove the solvent line that connects valve B to pressure sensor B from pressure sensor B and then monitor the pressure b Depending on the pressure do one of the following e If the pressure is below the threshold the solvent line is blocked Replace it Instrument Threshold pressure EASY nLC II 20 bar EASY nLC 1000 10 bar e Ifthe pressure is gt 20 bar pressure sensor B is blocked Replace it as described in Replacing a Pressure Sensor for the PLU Pump on page 90 260 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 6 Troubleshooting Troubleshooting the Autosampler Aspiration and Calibration Troubleshooting the Autosampler Aspiration and Calibration Troubleshooting the autosampler aspiration requires these tools and materials e 1 4 in open ended wrench e Powder free safety gloves e Sample vi
287. ry and keep the Log Entry dialog box open Press Add and Close to add this entry and close the Log Entry dialog box e Press Close to close the Log Entry dialog box without saving any entries To search the log In the Log Book list select Queue Job or User In the User List select Any Admin or Guest In the Time From list select a time In the Time To list select a time Press Search The search results appear in the Search Result table Checking and Resetting the Device Usage Counters The EASY nLC system tracks the volume pumped by pumps A B and S and the number of valve and rotor shifts for valves A B and S KD Kod l 2 To view the device usage values Press Maintenance gt Devices Do one the following e To view the volume pumped by a pump select the pump from the list of devices e To view the number of valve and rotor shifts for a valve select the valve from the list of devices Press the Summary tab The Summary view opens To reset the volume pumped or reset the rotor shifts to zero Open the appropriate Summary page Press Reset For a pump the value in the Intermediate Volume box resets to 0 For a valve the value in the Rotor Shifts box resets to 0 56 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific M Routine Maintenance To maintain the EASY nLC instrument follow the maintenance procedures in this chapter Tip You can access
288. s 1 To remove the stator from the valve assembly on page 79 2 To install the new stator on page 79 3 To remove air from the system and check for leaks after disassembling a valve on page 76 78 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific Thermo Scientific 4 Routine Maintenance Maintaining the Rotary Valves To remove the stator from the valve assembly Close down the EASY nLC system and then turn off the power to the instrument see Closing Down the EASY nLC Instrument on page 21 Using a 2 Phillips head screwdriver with a quarter turn loosen the three captive screws that secure the right side panel to the instrument housing Then remove the panel Disconnect the solvent lines from the valve as follows e Use a 1 4 in open ended wrench to remove stainless steel fittings e Use the black nanoViper knurled nut to loosen and remove nanoViper fittings Using a 9 64 in L hex wrench remove the two hex screws that secure the stator to the valve driver and then pull the stator away from the valve driver To install the new stator Mount the new stator onto the valve driver Using a 9 64 in L hex wrench tighten the two hex screws a little at a time by shifting from one screw to the other and back again until the screws are evenly torqued Reconnect the solvent lines to the valve as follows e For stainless steel fittings use a 1 4 in open en
289. s 10 Remove the PCB 11 Go to the next procedure To install the pump PCB on a PLF pump EASY nLC II instrument Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 167 5 Field Service Maintenance Replacing a Pump PCB To install the pump PCB on a PLF pump EASY nLC II instrument 1 Remove the damaged PCB as described in the previous procedure To remove the pump PCB from the PLF model pump EASY nLC II instrument on page 167 2 Using a small flathead screwdriver reset the address on the rotary switch on the new pump PCB as follows e Pump A l e Pump B 2 e Pump S 3 3 Reconnect the motor cable to the back of the PCB see Figure 178 on page 165 4 Mount the new PCB onto the pump 5 Secure the PCB to the pump as follows e Slide the four screws into the holes in the four corners of the PCB e Slide the connector at the end of the pressure sensor grounding cable under the screw in the lower right corner of the PCB e Using a 2 5 mm hex wrench tighten the four screws Figure 180 PLF pump with the pressure sensor cable connected grounding cable P Bus cable P Bus cable Motor cable routed to the back of the PCB CAUTION To avoid damaging the PCB make sure that the grounding connector does not touch any other components 6 Reconnect the P Bus communi
290. s Solvent System Schematic for the EASY nLC II Instrument Solvent System Schematic for the EASY nLC II Instrument Table 32 lists the tubing requirements for the EASY nLC II instrument The numbers in the ID column correspond to the numbers in the solvent system schematic shown in Figure 259 Kit LC230 includes the Teflon tubing and the check valve assembly required to replace the low pressure solvent system for one pump Kit LC243 includes a complete set of flow lines to the mixing Tee but does not include the mixing Tee itself Table 32 Tubing requirements for the EASY nLC II system No Connections Tubing Part number 1 Pump A outlet to pressure sensor A inlet PEEK 300 um ID 15 cm length LC212 Pump S outlet to pressure sensor S inlet Pressure sensor A outlet to port 1 of valve A Pressure sensor S outlet or port 2 of valve S 2 Pump B outlet to pressure sensor B inlet stainless steel prebent LC215 Pressure sensor B outlet to port 1 of Valve B stainless steel prebent LC216 4 Valve A to flow sensor A PEEKsil 50 um ID 25 cm length LC222 Valve B to flow sensor B 5 Flow sensor A to mixing Tee PEEKsil 20 pm ID 21 cm length Kit LC243 6 Flow sensor B to mixing Tee Fused silica 10 um ID 15 cm length 7 Mixing Tee to valve S PEEKsil 30 um ID 13 cm length 8 Autosampler needle connected to port 1 of valve S PEEKsil 150 um ID 55 cm length ASA model LC251 ASC model LC302 9 Column Out tubing connec
291. s page for the EASY nLC HPLC device on the Maintenance gt Devices page Make sure that the configured loop volume matches the installed sample loop EASY nLC Series Troubleshooting and Maintenance Guide 183 6 Troubleshooting Troubleshooting Tips Weak or Absent Signal Sample Not Aspirated A pressure drop at the start of the sample loading step indicates that pump S has introduced air into the sample loop see Figure 190 When the method uses pressure to control the flow rate the pressure should return to the specified level within approximately 4 seconds after the sample is loaded onto the column If the pressure does not return to the specified level within approximately 4 seconds pump S has probably introduced air into the system Figure 190 Typical pump A profile for a system with air in the sample loop M on Maintenance SCIENTIFIC a enalice Overview Pump A Graphs Desired flow Real flow Pressure Queue v o Flow nl min eq eunsseug tw e o Sampie 0015 33 Gradient 05 23 19 jul 2011 14 00 e admin a 8 9 Exit Time min Pressure drop at the start of the Sample Loading step ini fod 00 15 33 feet 184 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 6 Troubleshooting Troubleshooting Tips Weak or Absent Signal Defective Precolumn When compared to the Pump A graph for a normal run shown in Figure 185 on page 179 Figure 19
292. s Apply EASY nLC Series Troubleshooting and Maintenance Guide 289 8 Remote Support Connecting the EASY nLC Instrument to the Support Server 3 Send a support request message and a zip file containing the appropriate log files to Technical Support as follows a Press Maintenance gt Support The Support page of the Maintenance menu appears see Figure 252 Figure 252 Support page of the Maintenance menu Thermo Home Batch Setup Method Editor laintenance FESTE SCIENTIFIC Scripts Connect to EASY nLC support Log Book f Remote Port Number 2000 Connect Status Not Connected M Suppor f Message window can be used to chat with support when connected Show Clear Devices Log file copy To home directory To USB removable storage Copy Log Files Status Contact 00 00 00 Thermo Fisher Scientific easysupport proxeon com Edisonsvej 4 http Avww proxeon com hplic DK 5000 Odense C Phone 45 6557 2300 Denmark Fax 45 6557 2301 30 Aug 2010 14 14 admin Exit b In the Connect to EASY nLC Support area press Connect Depending on whether the instrument has the latest firmware file installed one of these dialog boxes appears e Ifthe instrument has the latest firmware file installed the Message to Support Team dialog box appears see Figure 253 Ifthe instrument does not have the latest firmware file installed the Upgrade is Available dialog box appears see Figure 256 on p
293. s and safety glasses when handling parts of the LC system that come into contact with solvents Replacing the pressure sensor that is connected to a PLF pump requires these items Tools Parts and materials e 2 Phillips screwdriver e Powder free gloves e 2 0 mm L hex wrench e Pressure sensor P N LC202 e 2 5 mm L hex wrench or ball driver e 1 4 in open ended wrench To replace a pressure sensor that is connected to a PLF pump follow these steps 1 To remove a pressure sensor that is connected to a PLF pump on this page 2 To install a pressure sensor for a PLF pump on page 121 To remove a pressure sensor that is connected to a PLF pump 1 Set the corresponding valve to the Center position as described in Using the Valve Controls on page 204 2 Close down the EASY nLC II instrument see Closing Down the EASY nLC Instrument on page 21 and unplug the power cable 3 Using a 2 Phillips head screwdriver with a quarter turn loosen the three captive screws that secure the right side panel to the instrument housing Then remove the panel Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 117 5 Field Service Maintenance Replacing a Pressure Sensor for a PLF Pump 4 Disconnect the P Bus communication cables from the pump see Figure 108 Figure 108 P Bus communication cables connected to the pump PCB of the PLF pump P Bus communication cables 5 Using a 2 5 mm L hex w
294. s from the threaded end of the nut IMPORTANT The inline filters have a swept volume flow path volume of less than one microliter To prevent leaks take care to properly seat the ferrule in the receiving port Once the ferrule is swaged onto the tubing do not connect the fitting to a different receiving port 4 Using a 5 16 in open ended wrench tighten the nut 5 Install the nanoViper solvent inlet line into the inline filter see Figure 70 Figure 70 Installed inline filters Inline filter 6 Run the Back Pressure script and the Leak script on the system as described in these topics e Test Back Pressure on page 48 e Test Leaks on page 44 82 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 4 Routine Maintenance Using nanoViper Fittings Using nanoViper Fittings For the EASY nLC instrument most of the plumbing connections are made with nanoViper fittings see Figure 71 Note For more information about working with nanoViper fittings see Using nanoViper Fittings Quick Reference Guide on page 323 Figure 71 nanoViper fitting Removable black PEEK sealing surface knurled tightening tool Screw Even though nanoViper fittings can withstand UHPLC backpressures of up to 1034 bar 15 000 psi they are fingertight fittings which require only very small torques to seal Therefore you must follow the guidelines below to
295. s of the System Leak Test Troubleshooting the Results of the System Leak Test Thermo Scientific Table 24 lists the actions required to troubleshoot the results of a system leak test Tip The Using nanoViper Fittings Quick Reference Guide on page 323 describes how to return the solvent lines in the flow path of the EASY nLC 1000 instrument to atmospheric pressure Print this reference guide to refer to while troubleshooting leaks coming from the nanoViper tubing connections Message System tight Sensor A lt 100nl min Table 24 Output messages for a system leak test and actions to take when this test fails Action No action is required however the system leak test does not test for a a leak in valve B or pump B Flow sensor A should be calibrated The 0 nL min point is off by more than 30 nL min so the software cannot determine leaks Rerun the system leak test If the same message appears run the Flow Sensor script to calibrate flow sensor A see Calibrate Flow Sensors on page 51 Flow sensor B should be calibrated The 0 nL min point is off by more than 30 nL min so the software cannot determine leaks Rerun the system leak test If the same message appears run the Flow Sensor script to calibrate flow sensor B see Calibrate Flow Sensors on page 51 Check valve S for leaks Flow sensor A gt 100 nL min Go to Checking Valve S for a Leak on page 22
296. s the two pump models To replace a pump piston seal and clean the piston follow these procedures 1 Retracting the Piston on page 61 2 Depending on the pump model follow one of these procedures e Replacing the Piston Seal and Cleaning the Piston in a PLF Pump on page 62 e Replacing the Piston Seal in a PLU Pump on page 67 Oo Priming the Pump on page 71 A Resetting the Pump Usage Counter on page 72 5 Removing Air After Replacing a Piston Seal or a Pump on page 73 6 Depending on whether you are performing maintenance on one of the chromatography pumps pump A or B or on the sample pump pump S follow one of these procedures e For pumps A and B run a pump leak test as described in Running the Leaks Script after Replacing a Piston Seal or a Pump on page 73 e For pump S run a sample pickup test as described in Test Sample Pickup on page 42 60 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific Retracting the Piston 4 Routine Maintenance Maintaining the Syringe Pumps Set the pump piston to the fully retracted position by running the Purge Solvent script with 0 iterations To retract the pump piston 1 On the touch screen press Maintenance gt Scripts 2 In the Category list select Prepare In the Name list select Purge Solvent 3 4 Press the Parameters tab 5 In the Purge Iterations box enter 0 see Figure 47
297. se hap were teei oes PAN Dee 89 Replacing a Pressure Sensor for the PLU Pump 00 200005 90 xiv EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific Contents Replacing a Flow Sensor seat esate ov keate whites ap beeteae baefe 92 Replacing a Flow Sensor in the EASY nLC 1000 Instrument 92 Replacing a Flow Sensor in the EASY nLC II Instrument 96 Replacing the Hard Drives sya aad 20 55 00 aah Ne ace an a aloes Ae aed 100 Managing the Devices List sccina oe waa det epes int C pr Eet AE ee eae 105 Chapter5 Field Service Maintenance 0 0ceeeee cece eee eee e eee 107 Replacing the Built In Computer 0 0 0 0 cee eee eee 108 Replacing the Monitor shes is ox eon iin Ohio ea phew e a onda Meanie 113 Replacing a Pressure Sensor fot a PLF Pump ew caid cv cae ea elena 117 Replacing the ASC Autosampler n o dues Satna s reeurar 122 Upgrading from an ASA Autosampler to an ASC Autosampler 128 Replacing the Autosampler Cooler n s 500k inka paw ereenn 137 Replacing the ASC Cooletsiccaso s cesie dan vo renea 137 Replacing the ASA Coolets isis ea anro eT Ae tee Reape steed 140 Replacing a Rotary Valve ioeis vacales Sedans teens edna weg ales 147 Replaciie a Pump vec Ge citoatt eid wee ee awe E outa 150 Replacing 4 PLU Pumpi nni ri n E eet a alee tay ede 150 Replacing a PLF Pump with a PLU Pump 00 00 0000 154 Replacing a Pump PCBarnvs si
298. seal tool P N LC210 CAUTION Wear powder free gloves and safety glasses when handling parts of the LC system that come into contact with solvents R To replace the piston seal in a PLF model pump 1 Ifyou have not already done so set the piston to its fully retracted position as described in Retracting the Piston on page 61 2 Set the corresponding valve to the Center position as described in Using the Valve Controls on page 204 3 Close down the EASY nLC instrument and turn off the power see Closing Down the EASY nLC Instrument on page 21 4 Using a 2 Phillips head screwdriver with a quarter turn loosen the three captive screws that secure the right side panel to the instrument housing Then remove the panel 62 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 4 Routine Maintenance Maintaining the Syringe Pumps 5 Remove the tubing connected to the pump head as follows e Ifa PEEK fitting is connected to the pump head use a 13 mm open ended wrench to remove it see Figure 48 Figure 48 Removing a PEEK fitting from the externally threaded pump head for a PLF model pump PEEK fitting connected to an externally threaded pump head e Ifa stainless steel fitting is connected to the pump head use a 1 4 in open ended wrench to remove it Figure 49 shows a stainless steel fitting connected to an internally threaded pump head Figure 49 Internally threaded pump he
299. see Figure 84 on page 94 4 Reinstall the inline filters on both ends of the flow sensor see Figure 83 on page 93 Tighten the fittings with an 8 mm open ended wrench 5 Reconnect the nanoViper tubing to both ends of the flow sensor see Figure 83 on page 93 6 Replace the right side panel 7 Turn on the EASY nLC 1000 system and wait one hour for the flow sensor to reach the correct operating temperature 8 Calibrate the new flow sensor by using the Flow Sensors script see Calibrate Flow Sensors on page 51 Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 95 4 Routine Maintenance Replacing a Flow Sensor Replacing a Flow Sensor in the EASY nLC II Instrument Replacing a flow sensor in the EASY nLC II instrument requires these tools and materials Tools Parts and materials e 2 Phillips head screwdriver e Powder free safety gloves e 2 5 mm hex wrench or ball e Flow sensor A type SLG1430 150 P N LC240 driver e Flow sensor B type SLG1430 025 P N LC241 CAUTION Wear powder free gloves and safety glasses when handling parts of the LC system that come into contact with solvents To replace an EASY nLC II flow sensor follow these steps 1 To remove an EASY nLC II flow sensor on this page 2 To install an EASY nLC II flow sensor on page 99 To remove an EASY nLC II flow sensor 1 Close down the EASY nLC system and then turn off the power to the instrument see Clo
300. sen the three captive screws that secure the right side panel to the instrument housing Then remove the panel Using a 1 4 in open ended wrench disconnect the stainless steel tubing connected to the pump head shown in Figure 162 Figure 162 Fitting on the pump head Fitting on the pump head EASY nLC Series Troubleshooting and Maintenance Guide 151 5 Field Service Maintenance Replacing a Pump 6 Depending on the instrument model do one of the following e For the EASY nLC 1000 instrument unplug the flow sensor cable from pump A or B the pressure sensor cable and the P Bus communication cables shown in Figure 163 Figure 163 Flow sensor cable pressure sensor cable and P Bus communication cables for an EASY nLC 1000 instrument One of four screws that secure the pump to the panel One of four screws that secure the pump to the panel Flow sensor cable Rotary switch One of four screws that secure the pump One of to the panel four screws that secure the pump to the panel Pressure sensor cable P Bus communication cables e For the EASY nLC II instrument unplug the pressure sensor cable and the P Bus communication cables from the pump The EASY nLC II flow sensors do not have a flow sensor cable that connects to the pump 7 Using a 3 mm ball driver or L hex wrench remove the four 3 mm hex screws that secure the pump to the instrument panel and then remove the pump Set the sc
301. sing Down the EASY nLC Instrument on page 21 2 Using a 2 Phillips head screwdriver with a quarter turn loosen the three captive screws that secure the right side panel to the instrument housing Then remove the panel 3 Using a 2 5 mm hex wrench remove the two screws see Figure 87 that secure the flow sensor bracket to the instrument panel 96 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 4 Routine Maintenance Replacing a Flow Sensor Figure 87 Removing the two screws that secure the flow sensor bracket to the panel w wv CMOSens SLG1430 150 Liquid Flow Sensor 0743 00078 4 gt Ser No max Flow 7000 nifmin 2 5 mm hex screw 4 Remove the solvent lines from both ends of the flow sensor see Figure 88 Figure 88 Removing the solvent lines Outlet ends Inlet ends Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 97 4 Routine Maintenance Replacing a Flow Sensor 5 Loosen the metal ring and remove the cable connected to the flow sensor see Figure 89 XY Figure 89 Removing the cable to the flow sensor Cable connected to the flow sensor 6 Using a 2 5 mm hex wrench remove the two screws that secure the flow sensor to the bracket see Figure 90 Figure 90 Removing the screws that secure the flow sensor to the bracket Two screws per flow sensor 98 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific
302. solvent levels 27 system for leaks 44 clogged column or system troubleshooting 178 closing down the EASY nLC instrument 21 Column Out line checking for blockage 253 clogged 178 180 tubing ID 249 column clogged 178 compliance FCC iii regulatory iii WEEE ix compliance electromagnetic v vii computer integrated location 15 out of memory 202 PC box replacing service repair 108 consumables 318 contact closure MS Connection script 39 troubleshooting 172 contamination declaration 301 preventing 205 cooler PCB 132 cooler temperature checking 28 direct control 211 daily maintenance 27 Declaration of Conformity v vii Declaration of Contamination 301 devices managing the Devices list 105 usage counters 56 Direct Infusion script 53 E EASY nLC 1000 instrument service repairs ASC autosampler replacing 122 127 ASC cooler replacing 137 139 high pressure valves replacing 147 149 monitor replacing 113 116 PC box replacing 108 112 PLU pump replacing 150 153 pump PCB replacing 163 166 336 EASY nLC Series Troubleshooting and Maintenance Guide EASY nLC II instrument service repairs ASA cooler replacing 140 146 ASC autosampler replacing 122 127 ASC cooler replacing 137 139 autosampler upgrading ASA to ASC 128 136 monitor replacing 113 116 PC box replacing 108 112 PLF pump replacing 154 pressure sensor replacing 117 121 pump PCB replacing 167 168 electromagnetic compatibility iii Exit button 21 F fa
303. sue code 11 Pump A stopped out of solvent Reported by Pump A Close When subsystem B has a large leak or is filled with air the gradient fails to start because the pressure cannot be increased to reach the starting conditions see Figure 195 Figure 195 Subsystem B leak SCIENTIFIC Overview Job 00 12 31 Sample 00 10 13 Gradient 00 00 20 Jul 2011 11 22 admin Exit 188 Desired flow Real flow Pressure 100 000 0 300 0 250 0 75 000 0 200 0 50 000 0 f 150 0 F 100 0 Flow nl min eq aunssaig 25 000 0 50 0 0 0 alk 0 0 0 0 25 50 75 10 0 Time min f Cradient 30 0 eF 100 0 90 0 250 0 t 80 0 70 0 t 60 0 150 0 p 50 0 40 0 30 0 t 20 0 10 0 0 0 200 0 Flow ni min gt gt gt o o gx eumixiw gy o 10 15 Time min EASY nLC Series Troubleshooting and Maintenance Guide Maintenance er Flow nl min Flow nl min nfiguration Pump B Desired flow Real flow Pressure 300 0 250 0 200 0 150 0 100 0 50 0 0 0 0 0 nss Time min Fras Desired flow Pressure 25 5 0 7 5 Time min 300 0 250 0 200 0 150 0 100 0 seq aunsseug 0 0 a aunsseig Pressure remains low Thermo Scientific 6 Troubleshooting Troubleshooting Tips When pump B cannot generate sufficient pressure to start the gradient the server error
304. t The script starts and the Output page opens Depending on the results of the Flush Air script displayed on the Output page do the following e Ifthe script passes run a system leak test as described in To run a system leak test on page 219 e Ifone of these messages appears go to Troubleshooting a Pump that Fails the Flush Air Script on page 212 Aborting unable to build pressure Aborting check solvent level CAUTION Wear powder free gloves and safety glasses when handling parts of the LC system that come into contact with solvents To run a system leak test If you have not already done so run the Flush Air script to prepare the instrument for a system leak test see To prepare the instrument for a system leak test on page 219 Connect the Column Out and Waste In lines with the appropriate union see able 23 on page 218 For the EASY nLC 1000 instrument connect these lines to the union as follows a Insert the nanoViper fittings on the ends of the Column Out and Waste In lines into the stainless steel union and then slowly tighten the fittings until you feel resistance EASY nLC Series Troubleshooting and Maintenance Guide 219 6 Troubleshooting Running a System Leak Test b Tighten both fittings by an additional 45 degrees 1 8 turn Do not tighten the fittings by more than a 1 4 turn see Using nanoViper Fittings on page 83 CAUTION Because the fittings seal aga
305. t Control and Laboratory Use Part 1 General Requirements EMC This Class A digital apparatus meets all requirements of the Canadian Interference Causing Equipment Regulations Thermo SCIENTIFIC European Safety and EMC Electromagnetic Compliance Standards Application of Council Directive s 2004 108 EC Electromagnetic Compatibility 2006 95 EC Low Voltage Directive LVD Standard s to which conformity is declared IEC 61010 1 Second edition 2001 Safety Requirements for Electrical Equipment for Measurement Control and Laboratory Use Also conformity with e EN 61010 1 2001 e UL61010 1 second edition e CAN CSA C22 2 No 61010 1 Second Edition EN IEC 61326 1 2006 Electrical equipment for measurement control and laboratory use EMC requirements Part 1 General requirements EN IEC 61000 3 2 2006 A1 A2 Electromagnetic compatibility EMC Part 3 2 Limits Limits for harmonic current emissions equipment input current lt 16 A per phase EN IEC 61000 3 3 2008 Electromagnetic compatibility EMC Part 3 3 Limits Limitation of voltage changes voltage fluctuations and flicker in public low voltage supply systems for equipment with rated current lt 16 A per phase and not subject to conditional connection FCC CFR 47 Part 15 Class A Radiated Emissions and Conducted Emissions Manufacturers Name Proxeon Biosystems A S Manufacturers Address Type of Equipment
306. t Thermo Fisher Scientific if neither action works us customer support analyze thermofisher com eu techsupport cms thermofisher com Copying to the USB memory stick does not stop Too much data to be copied Remove the memory stick from the EASY nLC instrument and press Ignore when the error message appears Contact Thermo Fisher Scientific for an instrument check up us customer support analyze thermofisher com eu techsupport cms thermofisher com Forgot the admin password If you are already logged in to the EASY nLC instrument Thermo Fisher Scientific can extract the password from the system in two ways e Ifthe network is set up for remote access press Maintenance gt Support and press Connect or e Runa factory reset System is running slower and slower Memory filled with graph data from long gradient run Press Home gt Graph and disable the graphs by pressing No Graph in each graph window System overload caused by other reasons 202 EASY nLC Series Troubleshooting and Maintenance Guide Restart the application or power down power up from Maintenance gt Scheduling Thermo Scientific Table 22 Miscellaneous troubleshooting tips Sheet 2 of 2 Symptom Possible causes Valve unknown position The valve s duty cycle requires calibration 6 Troubleshooting Troubleshooting Tips Action Run the Valve Check script see Test Valve Check on page 47
307. t the piston seal tool into the guide tool see Figure 55 Figure 55 Inserting the piston seal tool into the guide PLF model pump e Push the piston seal tool into the guide until you feel resistance see Figure 56 Figure 56 Seating the piston seal PLF model pump 66 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 4 Routine Maintenance Maintaining the Syringe Pumps f Remove the guide tube and the piston seal tool and then check that the piston seal is properly seated see Figure 57 Figure 57 Piston seal properly seated in the pump head PLF model pump 11 Using a 13 mm open ended wrench reconnect the pump head to the pump body 12 Go to Priming the Pump on page 71 Replacing the Piston Seal in a PLU Pump Replacing the piston seal in a PLU model pump requires these tools and materials Tools Parts and materials E 2 Phillips head screwdriver e Powder free safety gloves 3 mm L hex wrench e LC MS grade methanol e 1 4 in open ended wrench e LC MS grade acetonitrile e Pipette e Piston seal and piston seal tool P N LC510 To replace the piston seal in a PLU model pump 1 If you have not already done so set the piston to its fully retracted position as described in Retracting the Piston on page 61 2 Set the corresponding valve to the Center position as described in Using the Valve Controls on page 204 3 Close down the EASY nLC instrument and turn off
308. ta system computer refer to the EASY nLC Series Getting Started Guide b In the LAN area of the Configuration gt Network page do the following i Select LAN in the Profile list ii Press Apply To troubleshoot a failed connection to the support server 1 Check the support server configuration see step 2 on page 289 2 Verify that the Ethernet cable is securely connected to both the LAN port on the back panel of the EASY nLC system and the laboratory LAN port 3 Verify that the Ethernet cable is working 4 Ask your IT administrator if the network firewalls allow outgoing traffic to port 22 through an SSH connection 292 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 8 Remote Support Saving System Files on a USB Removable Storage Device Saving System Files on a USB Removable Storage Device The EASY nLC system includes network capability Through the network you can back up your system export and import batches and methods analyze your system by examining the log files and perform many other tasks However when you are not connected to the network or the network is broken or you do not have a computer with network access that is near the EASY nLC system you can use the copy function to copy all the system files on the system to a USB removable storage device such as a USB flash drive or memory stick The EASY nLC system ships with a USB flash drive for your convenience But you can use any US
309. ted to port 3 of valve S PEEKsil 30 pm ID 37 cm length LC260 10 Waste In line venting Tee to port 2 of valve W PEEKsil 75 pm ID 50 cm length LC262 11 Port 2 of valve A to check valve A Teflon 500 pm ID 15 cm length Kit Port 2 of valve B to check valve B Low pressure 12 Tubing from check valve A to solvent bottle A Teflon 500 pm ID 37 cm length solvent lines Tubing from check valve B to solvent bottle B LC230 13 Tubing from check valve A to the waste beaker Teflon 500 pm ID 39 cm length Tubing from check valve B to the waste beaker 14 Tubing from valve W to the waste beaker Teflon 500 pm ID 27 cm length LC263 314 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific C Consumables and Replacement Parts Solvent System Schematic for the EASY nLC II Instrument Figure 259 Solvent system schematic for the EASY nLC II system 2 2 a Solvent A Solvent B BB z Check valves M a for solvent A 13 Check valves for solvent B 00000000 00000000 00000000 00000000 00000000 00000000 000000 Flow sensor A Mixing Tee ressure sensor PEEK Stainless steel 1 1 PEEKsil B O Teflon Fused silica Pump S Pump A Pump B Pressure sensor Pressure sensor Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 315 C Consumables and Replacement Parts Solvent System Schematic for the EASY nLC 1000 Instrument Solvent System Schematic for the EASY nLC 1000 Instrument
310. tenance Scripts and Service Records Maintenance Scripts Torun the Sample Pickup script 1 Load the autosampler with the appropriate samples Tip For more information about running this script and testing the accuracy of the sample pickup function see Checking Sample Pickup on page 263 2 Open the Sample Pickup script as follows a Press Maintenance gt Scripts b In the Category list select Test c Inthe Name list select Sample Pickup 3 Set up the parameters for this script as follows a Press the Parameters tab b In the Volume box pL enter the volume to be picked up c In the Flow box pL min enter the flow rate that pump S uses to aspirate the sample For aqueous samples enter 20 pL min d In the Position box specify the position of the sample in the autosampler See the Position dialog box in Figure 33 4 Press Start Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 43 3 Maintenance Scripts and Service Records Maintenance Scripts Test Leaks The Leaks script tests for possible leaks at the pump seal and in the flow path from the pump to its associated valve high pressure subsystems A or B The Leaks script can also test for system leaks in the flow path downstream of valves A and B Use this script when you suspect leak problems in the pumps pump piston seals in the flow path between the pump outlet and the associated valve or downstream of valves A and B
311. terials Tools Parts and materials 1 4 in open ended wrench Powder free gloves e EASY nLC II instrument HPLC union P N SC600 e EASY nLC 1000 instrument HPLC union P N SC900 to loosen the stainless steel fittings CAUTION Wear powder free gloves and safety glasses when handling parts of the LC system that come into contact with solvents When running the Back Pressure script the system also checks for an unusually low back pressure If the back pressure is unusually low the system probably has a leak To troubleshoot a system blockage follow these procedures 1 Running the Back Pressure Test for the A and B Solvent Paths on page 250 2 Depending on the test results replace the blocked components in the column assembly or follow one or both of these procedures e Troubleshooting a System Blockage when Test Solvent A Fails on page 251 e Troubleshooting a System Blockage when Test Solvent B Fails on page 257 Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 249 6 Troubleshooting Troubleshooting a System Blockage Running the Back Pressure Test for the A and B Solvent Paths The Back Pressure script determines instrument back pressure for solvent lines A and B The script runs at a preset flow and measures the back pressure on the system IMPORTANT Before you perform the Back Pressure script ensure that the solvent A bottle contains water and the solvent B bottle contains ace
312. the Parameters tab e Select the check box for the appropriate pump f Press Start 2 As the pump is filling observe the waste line Ifyou observe solvent moving toward the check valve assembly the waste side check valve is leaking 3 If the waste side check valve is leaking replace it and repeat the Purge Solvent script To determine if the solvent side check valve is leaking 1 Start the Purge Solvent script 2 As the pump is emptying observe the solvent line If you observe solvent moving toward the solvent bottle from the check valve assembly the solvent side check valve is leaking 3 If the solvent side check valve is leaking replace it and repeat the Purge Solvent script 248 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 6 Troubleshooting Troubleshooting a System Blockage Troubleshooting a System Blockage Use this troubleshooting procedure when the back pressure for a chromatographic method rises above the expected level or when the flow decreases below the set rate for the column equilibration or sample loading steps In most cases an increase in back pressure is caused by a blocked column or a blocked Column Out line The Column Out line has an ID of 30 pm for the EASY nLC II instrument and 20 um for the EASY nLC 1000 instrument and is the smallest ID line that the sample passes through from sample pickup to the columns Troubleshooting a system blockage requires these tools and ma
313. the end of the 110 120 V line power text e Insert the 2 5 A fuse into the slot that aligns with the white triangle at the end of the 220 240 V text 6 Make sure that the fuse ends align with the mounting brackets Note The fuse holder holds two fuses One fuse is for a 220 240 V line power and the other is for a 110 120 V line power 7 Insert the fuse holder into the power entry module in the orientation that corresponds to the operating power The triangle to the right of the voltage rating points toward the white mark at the bottom of the power entry module see Figure 46 Figure 46 Power entry module A AOZL OLL AGSZ 30 ST 1GISN4 S30 PEE SELEUS IGA LEES AC FIRE LOF AOSZ HLM AINO 280 220 240 V Y Appropriate orientation for 220 240 line voltage Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 59 4 Routine Maintenance Maintaining the Syringe Pumps Maintaining the Syringe Pumps Each pump contains a piston seal and a piston Over time the buffered solutions leave deposits on the pistons and through constant contact with the moving pistons the piston seals slowly deteriorate Note Most EASY nLC II instruments contain PLF model pumps which have been discontinued If this pump is damaged you must replace the PLF model pump with a PLU model pump Both the EASY nLC 1000 instrument and new shipments of the EASY nLC II instrument use PLU model pumps Figure 8 on page 9 show
314. the flow sensors clear the Inspection Only check box and select the Exact Inspection option 3 Press Start Calibrate Reset Pressure Sensor This script auto zeroes the pressure sensor IMPORTANT Performing this script incorrectly will compromise the performance of the instrument Contact your local Thermo Fisher Scientific representative before running this script 52 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 3 Maintenance Scripts and Service Records Maintenance Scripts Calibrate Direct Infusion Use the Direct Infusion script to analyze samples by direct infusion or to optimize the spray stability by infusing a known peptide In a direct infusion experiment sample is introduced into the mass spectrometer without chromatographic separation by an analytical column The EASY nLC instrument picks up the sample volume from the specified location and then pumps it directly into the ion source using the solvent flow from pump A Figure 41 shows the parameters for the Direct Infusion script Figure 41 Direct Infusion script Thermo Home Batch Setup Method Editor SCIENTIFIC Scripts Category Name r z fe ib fa rust _ No graph a Log Book Calibrate Direct infusion Support Configuration Devices fro graph Job 00 04 20 17 Feb 2012 12 43 admin Exit To set up the system for a direct infusion experiment 1 Connect an em
315. the power see Closing Down the EASY nLC Instrument on page 21 4 Using a 2 Phillips head screwdriver with a quarter turn loosen the three captive screws that secure the right side panel to the instrument housing Then remove the panel Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 67 4 Routine Maintenance Maintaining the Syringe Pumps 5 Remove the used piston seal as follows a Using a 1 4 in open ended wrench remove the stainless steel fitting connected to the pump head see Figure 58 Figure 58 Stainless steel fitting connected to the pump head PLU model pump Stainless steel fitting b Using a 3 mm L hex wrench remove the two screws that secure the pump head to C the pump body see Figure 59 IMPORTANT Do not move the piston if the pump head has been removed Doing so can affect the piston alignment which might cause the piston calibration to be lost and result in irreversible piston damage Figure 59 Using a 3 mm L hex wrench to remove the pump head Two screws that secure the pump head to the pump body Clean the exposed portion of the piston with a lint free tissue soaked in HPLC grade methanol and visually inspect the piston for scratches Ensure that no solvent runs into the pump 68 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 4 Routine Maintenance Maintaining the Syringe Pumps d Using only your fingernails or a plasti
316. the system Contact Thermo Fisher Scientific if the problem recurs e us customer support analyze thermofisher com Pp y e eu techsupport cms thermofisher com Thermo Scientific Delayed Elution 6 Troubleshooting Troubleshooting Tips The most common cause of delayed elution is a worn rotor seal in valve B causing valve B to leak Table 12 lists the common causes of delayed elution These topics show the effect of leaks and swept volume on the pressure and flow traces e Leaks that Cause Delayed Elution on page 174 e Introduced Swept Volume on page 176 Table 12 Common causes of delayed elution Symptom Possible cause Delayed elution Leak in the solvent system Action 1 Run the Leaks script for the system see Test Leaks on page 44 2 Locate the leak by following Troubleshooting the Results of the System Leak Test on page 221 Incorrect tubing installed 1 Remove the right side panel of the instrument 2 Verify that the tubing matches the solvent system schematic e For the EASY nLC II instrument see page 314 e For the EASY nLC 1000 instrument see page 316 3 Replace the incorrect tubing with the specified tubing Swept volume introduced by incorrect tubing connections Thermo Scientific Check the tubing connections In the EASY nLC II instrument you can inadvertently introduce swept volume extra volume in the solvent path by not seating the tubing
317. the system is working go to step 4e e Reconnect all of the solvent lines and then run the Flush Air script again f Depending on the results of the Flush Air script do one of the following e Ifthe Flush Air script fails go to step 6 on page 214 e Ifthe Flush Air script passes you have completed this troubleshooting procedure Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 213 6 Troubleshooting Troubleshooting a Pump that Fails the Flush Air Script 5 Check whether the tubing that connects the pressure sensor to the valve is blocked as follows a Reconnect the solvent line to port 1 of the valve b Using a 1 4 in open ended wrench disconnect the fitting connected to the top of the pressure sensor c Pipette solvent into the port on the top of the pressure sensor CAUTION Be careful not to spill solvent on the PCBs e For the PLU pump in either the EASY nLC 1000 or EASY nLC II instrument the LED panel cover for each pump protects the PCB from accidental contact when the instrument s right side panel is removed However the cover does not form a waterproof seal with the pump body so that the PCBs are exposed to solvent leaks from above e For the PLF pump in the EASY nLC II instrument the PCBs are completely exposed d Run the Purge Solvent script and check that the pump is aspirating solvent through the pressure sensor e Depending on whether solvent is aspirated do one of the fo
318. the valves are in the appropriate position For information about controlling the position of the valves see Using the Valve Controls on page 204 IMPORTANT Before you fill pump A or B make sure that the pump s associated valve is in position 1 2 You can contaminate the pump by attempting to fill the pump when its associated valve is in position 1 6 To fill pump A or B with solvent 1 On the Home gt Overview page check the position of the valve that controls the solvent flow direction for the pump of interest 2 Make sure that the valve is set to position 1 2 When valve A or B is in the 1 2 position the corresponding pump draws solvent from the solvent bottle on top of the instrument see Figure 209 206 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 6 Troubleshooting Using the Direct Controls for Troubleshooting and Maintenance Figure 209 Position of the rotary valve when filling a pump Check valve assembly Solvent bottle A Waste beaker 3 Press the pump icon for the pump you want to control The Pump dialog box opens see Figure 208 4 In the flow rate box enter the filling flow rate 0 01 to 300 pL min For best results enter a flow rate of 100 pL min to fill the pump 5 In the volume box enter the volume that you want pump A or B to draw 6 Press Start The pump draws solvent until it draws the requested volume or reaches the full position whichev
319. ti i LSP disabled System controlled AANA Idle flow 250 30 113 7 140 0 pl Graphs eT 34 9 cuni Queue T E gt lt 2 4 bar lL ii 0 0 nl min D nI 7 140 0 140 0 ul Solvent A 1 2 bar 0 0 nl min 140 0 140 0 ul 12 4 nlfmin L SooToy 6 20 0 ul A etal Solvent B 00 07 49 f Eject plate f Insert plate f f sh refil 8 Jan 2013 Analytical col equili 14 15 Current 1 QG c On the Home gt Overview page check the readback for flow sensor A Then depending on the flow rate do one of the following e Ifthe flow rate is lt 100 nL min you have remedied the leak and completed this troubleshooting procedure Return the instrument to normal operation Ifthe flow rate is gt 100 nL min go to the next step Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 229 6 Troubleshooting Troubleshooting the Results of the System Leak Test 2 Make sure that the connection between the blind fitting and port 1 of valve W is leak tight as follows a Using a 1 4 in open ended wrench tighten the stainless steel blind fitting connected to port 1 of valve W b With the Analytical Column Equilibration script running monitor flow sensor A Then depending on the flow rate do one of the following Ifthe flow is lt 100 nL min you have remedied the leak and completed this troubleshooting procedure Return the instrument to normal operation e Ifthe flow is
320. ting connects to port 1 of valve S The tubing routes through a cutout in the panel that separates the solvent delivery system components from the autosampler compartment The other end of the tubing slides into the z axis holder and is secured with a PEEK nut 14 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 1 Introduction Hardware Components Computer and Autosampler Behind the Left Side Panel You can access the integrated computer and the autosampler needle fitting that connects to the needle guide by removing the left side panel of the instrument see Figure 15 Three captive quarter turn screws secure the left side panel to the instrument housing Figure 15 Internal features behind the left side panel ii Integrated computer Needle tubing Needle guide Autosampler Enclosed refrigerated sample compartment Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 15 1 Introduction Hardware Components Back Panel The system communication contact closure and power line connections are on the back y P panel of the instrument see Figure 16 and Figure 17 Figure 16 Back panel of the EASY nLC instrument prior to March 2012 PROXEON N OA do IN3
321. tion SCIENTIFIC Scripts Log record contains one term per line Time From Support Devices Search result Timestamp Source 05 08 2010 17 52 49 05 08 2010 17 44 34 19 07 2010 10 41 58 Log book First record Queue job 3 Time To ser Last record F any f Search Summary Batch admin 20100805 1751 Batch admin 20100805 1742 Batch admin 20100719 1039 Job 00 00 00 l4 2 05 201017 03 40 Purge solvent 00 00 00 29 04 2010 10 29 34 Purge solvent 00 00 00 ZS aug 20140 29 04 2010 10 28 42 12 37 Purge solvent 00 00 00 admin N BiN 434 f Exit f Enter Log Entry Press Enter Log Entry The Log Entry dialog box opens In the Templates list select a template In the box at the top of the dialog box enter a description of the service action The Templates list contains the following selections Comment New Part Maintenance Property Change and Precolumn Changed by lt User gt on lt Date gt In the Components list select a hardware component The Components list contains the following selections None Gradient Valve B Valve A Valve S Valve W Analytical Column Precolumn Autosampler Pump A Pump B and Pump S EASY nLC Series Troubleshooting and Maintenance Guide 55 3 Maintenance Scripts and Service Records Checking and Resetting the Device Usage Counters Do one of the following e Press Add to add this ent
322. tions Then run the Flush Air script If the Flush Air script fails see Troubleshooting a Pump that Fails the Flush Air Script on page 212 Leak in the pump Run the Leaks script for the affected pump If the Leaks script fails see Troubleshooting a Pump that Fails the Leaks Script on page 216 Figure 193 shows the leak test profile for pump A Even when the flow rate is set to the maximum allowable value the pressure for pump A does not increase Figure 193 Leak test profile for pump A SCIENTIFIC Maintenance Configuratior Overview Pump A Graphs Desired flow Real flow Pressure 110 000 0 a Queue 100 000 0 0 000 0 000 70 000 60 0 000 0 0 000 0 25 40 000 0 30 000 0 20 0000 1750 2 10 000 0 3 0 0 1500 z 10 000 0 G 20 000 0 2350 5 30 000 90 jod 19 03 35 40 000 0 Sample 00 07 18 50 000 0 gt Gradiemt 00 00 60 000 09 70 000 0 20 jut 2011 80 0000 1029 90 000 0 25 4 adinin 100 000 0 di r 110 000 0 gt gt gt nS 00 OS 10 18 20 25 30 35 40 IS SO SS 60 65 70 75 Exit Time min Extremely low pressure EASY nLC Series Troubleshooting and Maintenance Guide 187 6 Troubleshooting Troubleshooting Tips When subsystem A has a large leak pump A can run out of solvent during a sample run resulting in the server error message shown in Figure 194 Figure 194 Server error message displayed when pump A runs out of solvent 5 26 11 11 48 AM Is
323. ton draws solvent into the pump head and the forward movement of the piston pushes solvent into the attached high pressure solvent line The stepper motor controls the forward and backward movement of the piston within the pump head The pump body provides piston alignment The piston seal allows the piston to move freely within the pump head The piston seal is made of an extremely strong thermoplastic The seal for the EASY nLC 1000 pumps contains a wire spring that forces the inside flange of the seal against the piston to prevent leaks The seals are not leak proof In fact wetting the surface of the pistons requires a small quantity of liquid which acts as a lubricant to reduce wear on the piston seal Through continued use and over time the seal degrades and must be replaced Allowing the pump to run dry and neglecting to rinse the pump head assemblies after pumping buffered eluents shorten the lifespan of the seals The backup ring holds the piston seal in place It also provides better alignment of the piston 8 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 1 Introduction Hardware Components Figure 8 Syringe pump components showing the pump head removed from the pump body EASY nLC II syringe pump PLF model EASY nLC 1000 syringe pump PLU model j Pump head Piston seal Piston Backup ring Backup ring Rotary switch Pump body Stepper motor Motor cable The syringe pumps perform
324. tonitrile EASY nLC Series Troubleshooting and Maintenance Guide 265 6 Troubleshooting Troubleshooting Communication Problems Table 26 Possible causes of a network connection failure Symptom Possible cause The connection between the The network connection has EASY nLC instrument and the been unexpectedly disabled virtual interface for the Xcalibur data system fails Action Test the network connection as described in Testing the Network Connection to the EASY nLC Computer on page 266 Close down the EASY nLC instrument as described in Closing Down the EASY nLC Instrument on page 21 and then turn off the power Restart the Xcalibur data system computer Turn on the EASY nLC instrument and log in as an administrator Retest the connection An antivirus program is blocking port 6666 Test the connection Make sure that the data system computer is not connected to the Internet and then uninstall the antivirus program The network firewall is blocking port 6666 Test the connection Remove the firewall Connection between the data system computer and the EASY nLC instrument has been lost Test the connection Testing the Network Connection to the EASY nLC Computer If the connection between the EASY nLC instrument and the data system computer fails when you click Test Connection in the Thermo EASY nLC Configuration dialog box of the Foundation Instrument Configuration window follow
325. tonitrile This test is not valid for other solvents Torun the back pressure script for both the A and B solvent paths 1 Remove the columns and connect the Column Out line to the Waste In line using the appropriate HPLC union Instrument Union EASY nLC II SC600 EASY nLC 1000 SC900 2 Run the Back Pressure scripts for both Solvent A and Solvent B as follows a Press Maintenance gt Scripts b In the Category list select Test c Inthe Name list select Back Pressure d Press the Parameters tab e Select the Test Solvent A and Test Solvent B check boxes f Press Start 3 Depending on the test results do one of the following Ifthe Test Solvent A test fails and the following message appears go to Troubleshooting a System Blockage when Test Solvent A Fails on page 251 EASY nLC II instrument Pressure is xxx bar exceeds 100 bar limit test failed EASY nLC 1000 instrument Pressure is xxx bar exceeds 175 bar limit test failed Ifthe Test Solvent B test fails and the following message appears go to Troubleshooting a System Blockage when Test Solvent B Fails on page 257 Pressure is xxx bar exceeds 100 bar limit test failed Ifboth Test Solvent A and Test Solvent B tests pass and the following message appears inspect and if necessary replace the columns the packed emitter or both Pressure is xxx bar test passed 250 EASY nLC Series Troubleshooting and Maintenance G
326. trument replace the solvent line with a new fused silica line Then to confirm that the blockage was in the discarded solvent line repeat the Back Pressure script with the Test Solvent B check box selected For the EASY nLC 1000 instrument place valve B in position 1 2 to release the pressure Remove the blocked nanoViper line and replace it with a new nanoViper line Then to confirm that the blockage was in the discarded solvent line repeat the Back Pressure script with the Test Solvent B check box selected e Ifthe pressure is gt 20 bar keep pump B running and go to step 3 for the EASY nLC 1000 instrument or step 4 for the EASY nLC II instrument 258 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific Thermo Scientific 6 Troubleshooting Troubleshooting a System Blockage For the EASY nLC 1000 instrument to determine if the inline filter connected to the flow sensor outlet is blocked do the following a Remove the inline filter from the flow sensor outlet b Depending on the back pressure do one of the following e Ifthe pressure is lt 20 bar the inline filter is blocked Replace it with a new filter e Ifthe pressure is gt 20 bar reconnect the inline filter to flow sensor A and then go to step 4 To determine if flow sensor B is blocked do the following a Disconnect the solvent line from the inlet of flow sensor B and then monitor the pressure b Depending on the pressure do on
327. ttle Figure 10 Check valve connections From the solvent reservoir bottle Solvent side on the top of the instrument check valve Connected to port 2 of valve Aor B Waste side check valve Routed to the waste beaker The check valves are replaceable For more information about determining if the check valves are leaking see Identifying a Leaking Check Valve on page 248 EASY nLC Series Troubleshooting and Maintenance Guide 11 1 Introduction Hardware Components Six Port Rotary Valves The three position six port rotary valves consist of a driver a rotor seal and a stator see Figure 11 Figure 11 Valve components i Driver Stator Q Screws that C secure the stator Q to the dri KO o the driver The six port valves can be in three different positions 1 2 position 1 6 position and centered see Figure 12 Figure 12 Valve positions 1 2 position 1 6 position Centered 0 For maintenance purposes the system tracks the number of valve shifts for each valve see Checking and Resetting the Device Usage Counters on page 56 Depending on the valve position the valves perform the functions listed in Table 1 Note The EASY nLC solvent system contains three subsystems one for each pump A subsystem includes the pump the pressure sensor the solvent lines to and from the pressure sensor and the rotary valve 12 EASY nLC Series Troubleshooting and Maintenance Guid
328. uide 145 5 Field Service Maintenance Replacing the Autosampler Cooler 146 10 11 12 13 Using a 3 mm L hex wrench insert and tighten the four hex screws underneath the adapter plate holder Figure 153 on page 144 shows the location of these screws Using a 2 mm L hex wrench tighten the two screws in the small PCB Figure 151 on page 143 and Figure 152 on page 143 show the locations of these screws Using a 2 5 mm L hex wrench mount the lid where the ribbon cable connects to the autosampler cooler see Figure 150 on page 143 Insert the ribbon cable into the controller board see Figure 147 on page 142 and Figure 149 on page 142 Using a flat screwdriver or your fingernail push the cap around the ribbon cable in place see Figure 148 on page 142 Reattach the autosampler door see Figure 144 on page 140 Verify that the autosampler moves smoothly in and out by moving it manually Reconnect the power cable Turn on the EASY nLC II instrument and check the cooler temperature from the Home gt Overview page Before using the instrument install the vial plate adapter and perform an autosampler calibration For instructions see Chapter 7 Calibrating the Autosampler s XYZ Robot EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 5 Field Service Maintenance Replacing a Rotary Valve Replacing a Rotary Valve The procedure for replacing a rotary valve is the
329. uide Thermo Scientific 6 Troubleshooting Troubleshooting a System Blockage Troubleshooting a System Blockage when Test Solvent A Fails Follow this procedure when the Back Pressure test for Test Solvent A fails see Running the Back Pressure Test for the A and B Solvent Paths on page 250 To verify the system blockage 1 Run the Precolumn Equilibration script and check the back pressure as follows a On the Maintenance gt Scripts page select Prepare in the Category list and then select Precolumn Equil in the Name list b Press the Parameters tab c Inthe Volume pL box enter 140 pL d In the Flow pL min box enter 2 pL min e Leave the Max Pressure bar box blank f Press Start g Monitor the pump A pressure trace 2 Depending on the back pressure do the following Ifthe back pressure is below the pressure threshold the system blockage has been removed To confirm that the back pressure is below the pressure threshold rerun the Back Pressure script with the Test Solvent A check box selected e Ifthe back pressure is above the pressure threshold keep the Precolumn Equil script running and go to To locate the source of the blockage when the Test Solvent A test fails on page 253 Instrument Pressure threshold EASY nLC II 100 bar EASY nLC 1000 175 bar Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 251 6 Troubleshooting Troubleshooting a System Blockage Figur
330. uirements EMC This device complies with Part 15 of the FCC Rules Operation is subject to the following two conditions 1 This device may not cause harmful interference and 2 This device must accept any interference received including interference that may cause undesired operation WARNING Changes or modification to this unit not expressly approved by the party responsible for compliance could void the user s authority to operate the equipment Note This equipment has been tested and found to comply with the limits for a Class A digital device pursuant to Part 15 of the FCC Rules These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instruction manual may cause harmful interference to radio communications Operation of this equipment in a residential area is likely to cause harmful interference in which case users will be required to correct the interference at their own expense You must use shielded cables with this unit to ensure compliance with the Class A FCC limits Canadian Safety and EMC Electromagnetic Compliance Standards Safety This instrument has been reviewed for compliance with standard CAN CSA C22 2 No 61010 1 Second edition Safety Requirements for Electrical Equipment for Measuremen
331. ummary tab 4 Press Reset The value in the Intermediate Volume box resets to 0 For more information see Checking and Resetting the Device Usage Counters on page 56 72 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 4 Routine Maintenance Maintaining the Syringe Pumps Removing Air After Replacing a Piston Seal or a Pump To draw fresh solvent through the solvent system and to remove air from the solvent system components including the pump heads follow these procedures after you prime the pump and reset the usage counters To draw fresh solvent into the system and remove air from the system 1 To draw fresh solvent into the system and remove air from the solvent lines do the following a On the touch screen press Maintenance gt Scripts b In the Category list select Prepare c Inthe Name list select Purge Solvent see Prepare Purge Solvent on page 32 d Press the Parameters tab e In the Purge Iterations box enter 10 f Select the check box for the appropriate pump g Press Start and wait for the system to perform 10 purge cycles 2 To remove air from the pump head do the following a In the Name list select Flush Air see Prepare Flush Air on page 33 b Press the Parameters tab c Set the flush volume threshold to 10 pL for the PLF pump or 12 pL for the PLU pump Note Figure 8 on page 9 shows the PLU and PLF pump models d Press Start and
332. und pressure level lt 70 dBA Operating temperature 5 to 30 C Storage temperature 25 to 60 C Humidity 20 to 80 RH non condensing Sample viscosity 0 1 to 5 cP Solvent A Water with 0 1 formic acid Solvent B Acetonitrile with 0 1 formic acid Note Use only LC MS grade solvents Safety 24 EASY nLC Series Troubleshooting and Maintenance Guide According to IEC 61010 Thermo Scientific Thermo Scientific 1 Introduction Specifications Table 5 Physical specifications Item EASY nLC Il EASY nLC 1000 Width 35 cm 13 8 in 36 cm 14 2 in Depth 38cm 15 1 in 38cm 15 1 in Height 45 cm 17 7 in 45 cm 17 7 in Weight 32 kg 70 5 1b 35 kg 77 lb Weight in the shipping 45 kg 99 1b 45 kg 99 1b container Table 6 Electrical specifications Item Power requirements Specification 120 Vac 50 60 Hz 250 W 230 Vac 50 60 Hz 250 W For universal power supply UPS dimensioning assume 250 W Fuses For 120 Vac one T 5 AL 250 V fuse 5 x 20 mm IEC 60127 For 230 Vac one T 2 5 AL 250 V fuse All fuses are UL Listed and CSA certified Table 7 Communication specifications Item Contact closure Specification 3 outputs 3 inputs and 6 ground pins IN circuit TTL Level OUT circuit PhotoMOS relay protected against high voltages Continuous switch current from 0 8 to 5 V is 35 mA LAN 10 100 Mb s BaseT Ethernet USB 2 x USB 1 1 for keyboard and
333. up Method Editor SCIENTIFIC Es Category Name f fi fe i il No graph Log Book Prepare v Precolumn equil Support Configuration Output Devices f No graph Job Loses Max pressure bar 7 Sample 00 05 00 Gradient 00 00 2 May 2011 14 02 admin Schedule Exit 34 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific Thermo Scientific 3 Maintenance Scripts and Service Records Maintenance Scripts To run the Precolumn Equilibration script 1 Open the Precolumn Equilibration script as follows Press Maintenance gt Scripts In the Category list select Prepare In the Name list select Precolumn Equilibration 2 Set up the parameters for this script as follows Press the Parameters tab b Enter the appropriate values In the Volume pL box enter the volume of solvent A to use to equilibrate the precolumn For best results use at least 10 column volumes to equilibrate the precolumn In the Flow pL min box enter the flow rate for the precolumn equilibration step If the flow field is left empty the pump operates at the set pressure In the Max Pressure bar box enter the maximum allowed pressure If the pressure field is left empty the pump operates at the set flow as long as it is below the instrument maximum pressure of 300 bar for the EASY nLC II instrument or 1000 bar for the EASY nLC 1000 instrument If both a flow and a
334. ure sensor L bracket to the instrument panel see Figure 113 Figure 113 Pressure sensor L bracket screws Screws that secure the pressure sensor to the L bracket Screws that secure the L bracket to the instrument panel 10 Using a 2 mm L hex wrench or ball driver unscrew the two hex screws that secure the pressure sensor to the L bracket see Figure 113 120 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 5 Field Service Maintenance Replacing a Pressure Sensor for a PLF Pump To install a pressure sensor for a PLF pump 1 Disconnect and remove the pressure sensor from the EASY nLC I instrument as described in To remove a pressure sensor that is connected to a PLF pump on page 117 2 Using a 2 mm L hex wrench or ball driver insert and tighten the two hex screws to secure the pressure sensor to the L bracket 3 Using a 2 5 mm L hex wrench or ball driver insert and tighten the two hex screws to secure the pressure sensor L bracket to the instrument panel see Figure 113 on page 120 4 Reconnect the solvent lines to the pressure sensor see Figure 112 on page 120 5 Reconnect the pressure sensor communication cable being careful not to put any stress on the cables see Figure 111 on page 119 6 Gently slide in the PCB 7 Using a 2 5 mm L hex wrench or ball driver insert and tighten the four 2 5 mm hex screws to connect the PCB to the pump see Figure 109 on page 118
335. ust accept any interference received including interference that may cause undesired operation WARNING Changes or modification to this unit not expressly approved by the party responsible for compliance could void the user s authority to operate the equipment Note This equipment has been tested and found to comply with the limits for a Class A digital device pursuant to Part 15 of the FCC Rules These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instruction manual may cause harmful interference to radio communications Operation of this equipment in a residential area is likely to cause harmful interference in which case users will be required to correct the interference at their own expense You must use shielded cables with this unit to ensure compliance with the Class A FCC limits Canadian Safety and EMC Electromagnetic Compliance Standards Safety This instrument has been reviewed for compliance with standard CAN CSA C22 2 No 61010 1 Second edition Safety Requirements for Electrical Equipment for Measurement Control and Laboratory Use Part 1 General Requirements EMC This Class A digital apparatus meets all requirements of the Canadian Interference Causing Equipment Regulations Europ
336. utosampler needle a j New f Calibrate Current amp x1 2Angene AB 800 Calibrate 5 1 Mount the new autosampler needle in the z axis holder Check G Manipulator f A Bxt2 Abgene a8 800 ii that the small spring is situated between the PEEK nut and the eset SA 9 Cc a plastic stop 2 Do one of the following For the ASC autosampler go to step step 3 dfs the Manipulator atta pres Reset e For the ASA autosampler go to step step 4 The z axis needle holder moves to the W1 position 3y Forte ASC anosmples dothefolowtie 7 Using a 2 Phillips screwdriver loosen the three quarter turn screws that secure the panel to the left side of the EASY nLC instrument and then remove the panel a Guide the end of the needle that connects to Valve S through the large hole in the metal plate that separates the tray compartment from the solvent system compartment 8 Do one of the following b Install the black needle holder in the side plate For the ASC model remove the black needle holder from the 7 For the ASA autosampler slide the valve end of the tubing slot in the panel that separates the solvent system hardware through the small plastic holder on the back of the touch screen from the autosampler compartment Removing the fitting a requires some gentle movement from both sides of the plate Figure Holderonihe bankai the toich saan moni Figure 6 Removing the black needle holder fr
337. utosampler needle P N LC251 e ASC autosampler needle P N LC302 To remove the autosampler needle from an ASC or an ASA model autosampler 1 Using a 2 Phillips head screwdriver with a quarter turn loosen the three captive screws that secure the right side panel to the instrument housing Then remove the panel 2 Using a 1 4 in open ended wrench unscrew the nut that secures the autosampler needle to port 1 of valve S Then remove the fitting from the port Figure 72 and Figure 73 show the solvent line connections to valve S for the EASY nLC II solvent system and the EASY nLC 1000 solvent system respectively Figure 72 Valve S solvent line connections for the EASY nLC II instrument Valve S Autosampler needle From the pressure sensor Column Out line From the mixing Tee Figure 73 Valve S solvent line connections for the EASY nLC 1000 instrument Valve S Autosampler needle i From the pressure sensor Column Out From the line ie mixing Tee 84 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 4 Routine Maintenance Replacing the Autosampler Needle 3 Remove the nut ferrule and sleeve from the needle tubing Figure 74 Autosampler tubing with a nut ferrule and sleeve at one end 4 Depending on the instrument model move the z axis needle holder to an accessible location within the tray compartment by doing the following For th
338. vanced Mass Spectrometry Customer Search for keyword Registration Customer Information Model EASY nLC Service Instrument Partner Program Part Number Reset to the Default Parameters 5 Open a list of linked PDF documents for the EASY nLC as follows a In the Model list select EASY nLC and in the Document Type list select Manual b Click Submit Search XX EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific Preface 6 On the Documents page click the link to the right of Download to view the PDE 7 Click the Save a Copy icon B browse to an appropriate folder and then click Save to save a copy of the document Safety and Special Notices Make sure to follow the safety practices presented in this guide and observe the safety and special notices that appear in boxes Observe all written safety precautions during all phases of operation service and repair of this instrument Failure to comply with these precautions or with specific warnings elsewhere in this manual violates safety standards of design manufacture and intended use of the instrument and might result in injury or loss of life The following two stickers appear on the EASY nLC instrument This sticker warns you that the instrument includes a sharp needle and moving parts that are accessible to the operator To prevent personal injury or damage to parts of the EASY nLC instrument
339. vent Lines The following solvent lines numbered from left to right in Figure 3 are in the flow path upstream of valve S Solvent line Solvent line 1 Port 6 of valve A to flow sensor A inlet 4 Flow sensor B outlet to mixing Tee inlet 2 Port 6 of valve B to flow sensor B inlet 5 Mixing Tee outlet to port 4 of valve S 3 Flow sensor A outlet to mixing Tee inlet 6 Sample loop connected to ports 2 and 5 of valve S gt To return the solvent lines that are upstream of valve S to atmospheric pressure 1 On the touch screen press Home gt Overview 2 Using the valve controls set the valves to the following positions Valve Position Effect A and B Center Maintains the pressure between the pump A and valve A during the Analytical Column Equilibration script S and W 1 6 Vents the system pressure downstream of valve A Figure 3 shows the flow path when valves A and B are Centered and valves S and W are in position 1 6 Figure 3 Solvent lines that are downstream of valves A and B and upstream of valve S numbered 1 6 No flow Center position 325 Waste beaker Downstream of Valve S Solvent Lines 326 The following solvent line connections are in the flow path downstream of valve S e Column Out tubing connected to port 3 of valve S and the HPLC union P N SC900 e Waste In tubing connected to port 2 of valve W and the HPLC union or the venting Tee gt To return the solvent lines t
340. w in the lower right corner Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 143 5 Field Service Maintenance Replacing the Autosampler Cooler 9 Using a 3 mm L hex wrench loosen the four hex screws underneath the adapter plate holder and remove the holder Figure 153 shows the location of these screws Figure 153 Screws beneath the adapter plate holder 10 Lift and pull the adapter plate holder out of the autosampler as shown in Figure 154 Figure 154 Pulling the adapter plate holder out of the autosampler EASY nbc o Note The sleeves around the holes on the bottom of the adapter plate holder can fall out Figure 155 shows these sleeves Figure 155 Hole sleeves on the bottom of the adapter plate holder A Sleeve that has fallen out of the hole 144 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 5 Field Service Maintenance Replacing the Autosampler Cooler To install the ASA cooler 1 Align the arms of the autosampler bed as shown in Figure 156 Figure 156 Aligning the arms of the autosampler bed 2 Carefully place the new cooler in the autosampler bed as shown in Figure 157 Figure 157 Placing the new cooler in the autosampler bed 3 Verify that the cables from the fan are routed in the groove see Figure 158 Figure 158 Verifying the placement of the fan cables Thermo Scientific EASY nLC Series Troubleshooting and Maintenance G
341. wait for the script to finish Running the Leaks Script after Replacing a Piston Seal or a Pump Thermo Scientific After you prime pump A or pump B reset the usage counters remove air from the system and run a pump leak test Note For pump S run a sample pickup test as described in Test Sample Pickup on page 42 For information about replacing a pump see Replacing a Pump on page 150 Only a Thermo Fisher Scientific field service engineer can replace a damaged pump as replacing the pump is an advanced maintenance procedure Torun a pump leak test on a replacement pump or a pump with a new piston seal Follow the instructions in Test Leaks on page 44 When the instrument is leak tight the instrument is ready for use EASY nLC Series Troubleshooting and Maintenance Guide 73 4 Routine Maintenance Maintaining the Rotary Valves Maintaining the Rotary Valves The EASY nLC instrument has four rotary valves labeled Valve A Valve B Valve S and Valve W These valves contain the same internal components To determine if the valve requires maintenance run the Leak test script as follows e For valves S and W run the Leaks script on the system System e For valves A and B run the Leaks script on the respective subsystem A or B To maintain the valves follow these procedures e Cleaning the Rotor Seal and Stator on this page e Replacing the Rotor Seal on page 77 e Replacing the
342. ween the EASY nLC 1000 system and the EASY nLC II system Depending on the instrument model follow one of these procedures to replace a flow sensor e Replacing a Flow Sensor in the EASY nLC 1000 Instrument on this page e Replacing a Flow Sensor in the EASY nLC II Instrument on page 96 Replacing a Flow Sensor in the EASY nLC 1000 Instrument Replacing a flow sensor in the EASY nLC 1000 system requires these tools and materials Tools Parts and materials e 2 Phillips head screwdriver e Powder free safety gloves e 8 mm open ended wrench e Flow sensor A B P N LC540 e 2 5 mm hex wrench or ball driver CAUTION Wear powder free gloves and safety glasses when handling parts of the LC system that come into contact with solvents 92 EASY nLC Series Troubleshooting and Maintenance Guide Thermo Scientific 4 Routine Maintenance Replacing a Flow Sensor To replace a flow sensor in the EASY nLC 1000 system follow these steps 1 To remove an EASY nLC 1000 flow sensor on this page 2 To install an EASY nLC 1000 flow sensor on page 95 To remove an EASY nLC 1000 flow sensor 1 Close down the EASY nLC system and then turn off the power to the instrument see Closing Down the EASY nLC Instrument on page 21 2 Using a 2 Phillips head screwdriver with a quarter turn loosen the three captive screws that secure the right side panel to the instrument housing Then remove the panel 3 Remove the nanoViper tu
343. x T 8 wrench to remove the screws Place the rod back in the drawer afterward Figure 96 Removing the brass rod that holds the cable down To install the hard drive 1 Insert the new hard drive 2 Connect the hard drive to the IDE cable Qe Insert the four screws into the holes underneath the hard drive drawer and then using a Torx T 10 wrench tighten the four screws to secure the hard drive to the drawer see Figure 94 on page 102 4 Close the hard drive drawer Nn Using a 2 5 mm L hex wrench insert and tighten the two screws that secure the drawer to the back panel see Figure 92 on page 101 6 Turn on the EASY nLC instrument 7 Press Vendor 8 When prompted enter the serial numbers for the instrument and the hard drive Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 103 4 Routine Maintenance Replacing the Hard Drive Note When you replace the hard drive the pumps and valves are automatically detected during the boot procedure Because the autosampler is not detected by the boot procedure you must add it to the Device list After you add the autosampler to the Device list you must calibrate the plate formats that you plan to use and the wash bottle positions as these calibrations were stored on the old hard drive 9 Add the autosampler to the Devices list as follows a Press Maintenance gt Devices b Press Add Device The Select a Device to Add dialog box op
344. ying pumps A and B and places the autosampler needle in the appropriate wash and waste bottles for filling and emptying pump S To work with the pump s direct controls follow these procedures e Opening the Direct Controls for the Pumps e Using the Direct Controls To Fill or Empty Pump A or B e Using the Direct Controls To Fill or Empty Pump S Opening the Direct Controls for the Pumps Each pump is represented by a pump icon on the Home gt Overview page To open the pump dialog box On the Home gt Overview page press the pump icon for the pump that you want to control Its dialog box opens see Figure 208 The Position readback box displays the current pump position from 0 pL empty to 140 pL full Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 205 6 Troubleshooting Using the Direct Controls for Troubleshooting and Maintenance Figure 208 Pump dialog box Pump A Pump A Volume box 0 55 pirin Flow rate box aS gt S gt S gt gt e gt a 140 0 ul St Start Pressure 22 3 Bar Position 15 84 pl Readback boxes Real flow 549 6 nl min a Close Note Because pump S does not have an associated flow sensor the Pump S dialog box does not include a Real Flow readback box Using the Direct Controls To Fill or Empty Pump A or B Before using the direct controls to fill or empty pump A or B make sure that
345. you have not already done so prepare the autosampler for the extra vial and wash bottle calibration as described in To prepare the autosampler for the V1 and W4 calibration routine on page 278 2 Open the Autosampler Vials Wash Calibration dialog box as follows a Press Maintenance gt Devices b In the Devices list select the autosampler c Press the Tools tab below the Devices list d In the Vials Wash area press Calibrate The Autosampler Vials Wash Calibration dialog box opens see Figure 249 3 Press the bottle container in W4 1 see Figure 249 The needle moves to the current saved coordinate The saved coordinates for the x and y axis appear on the left side of the slash the needle does not descend into the bottle and the z axis value on the left side of the slash remains at 0 Figure 249 Vials Wash view in the Autosampler Vials Wash Calibration dialog box Calibrate Vials Wash Needle Infout Z Needle Up Down 4 3 Needle LeftRight Reset 00 00 D 00 00 OE Ble Thermo Scientific EASY nLC Series Troubleshooting and Maintenance Guide 285 7 Calibrating the Autosampler s XYZ Robot Calibrating the Wash Bottle and Extra Vial Positions 4 Center the needle above the bottle as follows Press the Needle In Out 2 buttons to adjust the x axis position Press the Needle Left Right 3 buttons to adjust the y axis position X axis Out Right

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