OPERATING INSTRUCTIONS AND SYSTEM DESCRIPTION FOR
Contents
1. BNC connector for the electrode holder driven shield 2 GND ground connector 3 HV TR150 headstage connector and cable to the HV TR150 4 BNC connector and cable to the headstage of the recording amplifier e g BA 01M OPERATE LED indicates that injection current flows holding bar NN Caution The current injection headstage has an output compliance of 125 V if switch 3 Figure 4 is in HV position 1 e 1f the OPERATE LED lights In addition all headstages are equipped with sensitive FET amplifiers that can be damaged by electrostatic charge and must therefore be handled with care see also chapter 1 Very Important Always turn power off when connecting or disconnecting headstages or when changing or adjusting the electrodes version 1 8 page 9 HV TR150 User Manual 3 6 Operation In combination with an amplifier Injecting a tracer after an experiment with an intracellular amplifier e g BRAMP O1R using continuous current E Connect the headstage of the BRAMP OIR to the headstage of the HV TR150 see Figure 6 Connect the recording application electrode to the headstage of the HV TR150 see Figure 6 Set switch 3 to AMP The green LED 2 lights Do your experiment as usual with the BRAMP OIR After finishing set switch 3 to HV Select the desired current amplitude with potentiometer 12 and the polarity of the injection current using switch 11 Set switch 4 to CONT to start tracer injection DO ELE F2. induction of electromagnetic noise the power supply unit the power switch and the fuse are located at the rear of the housing see Figure 2 right 2 6 PWR 03D The external power supply PWR 03D is capable of driving up to 3 EPMS E housings Each housing 1s connected by a 6 pole cable from one of three connectors on the front panel of the PWR 03D to the rear panel of the respective EPMS E housing see Figure 3 Fehler Verweisquelle konnte nicht gefunden werden A POWER LED indicates that the PWR 03D is powered on see Figure 3 left Power switch voltage selector and fuse are located at the rear panel see Figure 3 right Note The chassis of the PWR 03D is connected to protective earth and it provides protective earth to the EPMS E housing if connected pow TRAC y BSD Ha paac hy aie Ta mi CE Dx torn prerai we ORO C N CONT Jan ii E E Figure 3 Left PWR 03D front panel view Right PWR 03D rear panel view Note This power supply is intended to be used with npi EPMS E systems only version 1 8 page 5 HV TR150 User Manual 3 HV TR150 Tracer Injection Module 3 1 HV TR150 Components The following items are shipped with the HV TR150 system Y Injection module for the EPMS 07 system Y Headstage Y User manual Optional accessories gt Electrode holder see also Figure 5 and Figure 6 3 2 HV TR150 System Description The HV TR150 tracer injector is a plug in unit for the npi EPMS H 07 modular sy
3. or treatment of humans or for any other life supporting system npi electronic disclaims any warranties for such purpose Equipment supplied by npi electronic must be operated only by selected trained and adequately instructed personnel For details please consult the GENERAL TERMS OF DELIVERY AND CONDITIONS OF BUSINESS of npi electronic D 71732 Tamm Germany 1 2 3 4 5 GENERAL This system is designed for use in scientific laboratories and must be operated only by trained staff General safety regulations for operating electrical devices should be followed AC MAINS CONNECTION While working with the npi systems always adhere to the appropriate safety measures for handling electronic devices Before using any device please read manuals and instructions carefully The device is to be operated only at 115 230 Volt 60 50 Hz AC Please check for appropriate line voltage before connecting any system to mains Always use a three wire line cord and a mains power plug with a protection contact connected to ground protective earth Before opening the cabinet unplug the instrument Unplug the instrument when replacing the fuse or changing line voltage Replace fuse only with an appropriate specified type STATIC ELECTRICITY Electronic equipment is sensitive to static discharges Some devices such as sensor inputs are equipped with very sensitive FET amplifiers which can be damaged by electrostatic charge and must therefore
4. to be additive see chapter 3 4 it is possible to generate a RETAIN current additionally 3 Select the desired RETAIN current amplitude with potentiometer 12 and the polarity of the RETAIN current using switch 11 Leave switch 4 to CONT version 1 8 page 11 HV TR150 User Manual 4 Technical Data Electrode output Maximum current Output current polarity Output current amplitude Modes of operation Display Analog input Current monitor Voltage monitor Size Headstage size EPMS 07 SYSTEM Dimensions version 1 8 floating current source output impedance gt 10 Q 1 25 uA into 100 MQ load selected by 0 toggle switch selected by 10 turn potentiometer or analog input set by two toggle switches or TT L signal CONT GATE switch enables manual or TTL gated operation AMP HV switch connects the recording application electrode output to amplifier s headstage or to HV TR150 current source output current XXXX nA sensitivity 100 nA V Rin gt 100 kQ range 10 V sensitivity 100 nA V Rout 250 Q not isolated Ver 10 Rout 250 Q not isolated front panel 12 HP 60 6 mm x 3U 128 5 mm 7 175 mm deep 65x25x25 mm Power Requirements 115 230 V AC 60 50 Hz fuse 2 A 1 A slow 45 60 W dependent on the modules plugged in 19 rackmount cabinet 3U high 1U 1 3 4 44 45 mm page 12
5. Electronic Instruments for the Life Sciences ASU e ade Te OPERATING INSTRUCTIONS AND SYSTEM DESCRIPTION FOR THE HV TR150 HIGH VOLTAGE TRACER INJECTION MODULE FOR EPMS SYSTEMS E Hv Tr150 npi VERSION 1 8 npi 2015 npi electronic GmbH Bauhofring 16 D 71732 Tamm Germany Phone 49 0 7141 9730230 Fax 49 0 7141 9730240 supportOnpielectronic com http www npielectronic com HV TR150 User Manual Table of Contents Is SAFE hye UI ii dida 3 2 EPMS07 Modular Plus lt I systems 4 2 1 General System Description OperatiON oooocccncncconnnonnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnannncnnnnnnnnnos 4 22s STA A AN 4 A A E E aca 4 Zi US AAA O a en te UR Ee 4 2 Se AN 5 ZO PWR SO SD ina ini 5 ox tae RADU Tracer Injection Module oee G 6 31s HV TRIO OMpOMe mess seian a ti 6 32 HV LRIS0 System Descriptio no 6 J Descripuon ot Me Front Pane rse AA 7 SA TN 9 NS A O eI Rem eR PID nee eR een 10 In combination with an amplifier cocccccnnonnnocnnnnnnnnnnnnnnanonnncnnnnnnonnnonnnncnnnonononnnos 10 As independent iontophoresis amMplifleT ooooccccnncncnnnnnoonnnnnnnnnnononanonnnnnnnnnononaninonoss 11 A Ne hea teea eee hare uted sata haccacd te ca tae cee tues a tas hancaneneeala tons 12 version 1 8 page 2 HV TR150 User Manual 1 Safety Regulations VERY IMPORTANT Instruments and components supplied by npi electronic are NOT intended for clinical use or medical purposes e g for diagnosis
6. amplitude selected with potentiometer 12 and can be used either in GATED or CONT mode Scaling 100 nA V For instance when using as independent iontophoresis instrument a RETAIN current can be set by potentiometer 12 and switch 11 and a signal generated by a data acquisition system can be connected to the analog IN connector providing the EJECT current 8 HS connector Connector for the headstage 9 Analog OUT connector BNC connector monitoring the injection current Scaling 1 V 100 nA 1 e 1 V output corresponds to 100 nA injection current 10 VeL 10 connector BNC connector providing the potential in Volt at the electrode divided by ten 11 0 switch Switch for selecting the polarity of the injection current 12 Amplitude potentiometer Potentiometer for selecting the amplitude of the injection current The amplitude can always be read at the CURRENT nA display 1 Note The value selected at 12 is added to the signal at Analog IN connector 7 version 1 8 page 8 HV TR150 User Manual 3 5 Headstage The headstage is housed in a small box that can be mounted directly onto a micromanipulator It is connected to the HV TR150 by means of a shielded flexible cable and a multi pole connector and it is connected to the headstage of the recording amplifier e g BRAMP OIR using a shielded flexible cable and a BNC connector Figure 5 HV TR150 headstage with electrode holder optional 1 ELECTRODE
7. be handled with care Electrostatic discharge can be avoided by touching a grounded metal surface when changing or adjusting sensors Always turn power off when adding or removing modules connecting or disconnecting sensors headstages or other components from the instrument or 19 cabinet TEMPERATURE DRIFT WARM UP TIME All analog electronic systems are sensitive to temperature changes Therefore all electronic instruments containing analog circuits should be used only in a warmed up condition 1 e after internal temperature has reached steady state values In most cases a warm up period of 20 30 minutes is sufficient HANDLING Please protect the device from moisture heat radiation and corrosive chemicals version 1 8 page 3 HV TR150 User Manual 2 EPMS 07 Modular Plug In System 2 1 General System Description Operation The npi EPMS 07 is a modular system for processing of bioelectrical signals in electrophysiology The system is housed in a 19 rack mount cabinet 3U has room for up to 7 plug in units The plug in units are connected to power by a bus at the rear panel The plug in units must be kept in position by four screws M 2 5 x 10 The screws are important not only for mechanical stability but also for proper electrical connection to the system housing Free area must be protected with covers 2 2 EPMS 07 Housing The following items are shipped with the EPMS 07 housing V EPMS 07 cabinet with built in powe
8. nd then to activate the current flow 2 AMP HV LEDs LEDs indicating which module is connected to the electrode when operating in combination with an amplifier module using the same electrode AMP the amplifier module e g BA 01M is connected to the electrode HV the high voltage module HV TR150 is connected to the electrode version 1 8 page 7 HV TR150 User Manual 3 AMP HV switch Toggle switch for selecting the module that is connected to the electrode AMP the amplifier module e g BRAMP 01R is connected to the electrode HV the high voltage module HV TR150 is connected to the electrode 4 CONT GATE switch Toggle switch for selecting continuous CONT position or gated GATE position operation 5 TTL IN connector BNC connector for selecting the module that is connected to the electrode remotely with a TTL signal HI the high voltage module HV TR150 is connected to the electrode LOW the amplifier module e g BRAMP 01R is connected to the electrode Note AMP HV switch 3 must be in AMP position for remote selection 6 TTL IN connector BNC connector for the gating signal in GATED operation CONT GATE switch in GATE position The injection current is generated as long as the TTL signal is HI 5 V 7 Analog IN connector BNC connector for an external waveform e g from a data acquisition system or a function generator The signal connected here is added to the current
9. or gated operation set switch 4 to GATE and apply a gating TTL signal to connector 5 Note If the HV TR150 is active the display of the BRAMP OIR shows any value because the headstage of the recording amplifier is not connected to the electrode 1 e is open Figure 6 connection of BA 01M and HV TR150 headstages version 1 8 page 10 HV TR150 User Manual As independent iontophoresis amplifier Injecting a dye iontophoretically E 3 E 3 Connect the application electrode to the headstage of the HV TR150 Set switch 3 to HV The yellow LED 2 lights Select the desired current amplitude with potentiometer 12 and the polarity of the injection current using switch 11 Set switch 4 to CONT to start dye injection For gated operation set switch 4 to GATE and apply a gating TTL signal to connector 6 The voltage at the electrode and the injection current can be monitored using connectors 9 and 10 Injecting a dye iontophoretically using a data acquisition system or a function generator DO OO OL Connect the application electrode to the headstage of the HV TR150 Set switch 3 to HV The yellow LED 2 lights Set switch 11 to O and switch 4 to CONT Connect the output of a data acquisition system or a function generator to connector 7 and program a current injection protocol Start current injection using the data acquisition system or the function generator Since input 7 and the potentiometer 12 are wired
10. r supply Y Mains cord J Fuse 2 A 1 A slow inserted Y Front covers Figure 1 Left front view of empty EPMS 07 housing In order to avoid induction of electromagnetic noise the power supply unit the power switch and the fuse are located at the rear of the housing see Figure 2 right 2 3 EPMS H 07 Housing In addition to the standard power supply of the EPMS 07 the EPMS H 07 has a built in high voltage power supply This 1s necessary for all MVCS MVCC modules the HVA 100 HV TR150 and HVC 03M modules The output voltage depends on the modules in use 2 4 EPMS E 07 Housing The following items are shipped with the EPMS E 07 housing EPMS E 07 cabinet External Power supply PWR 03D Power cord PWR 03D to EPMS E 07 Mains chord Fuse 1 6 A 0 8 A slow inserted Front covers SNA A K S version 1 8 page 4 HV TR150 User Manual The EPMS E 07 housing is designed for low noise operation especially for extracellular and multi channel amplifiers with plugged in filters It operates with an external power supply to minimize distortions of the signals caused by the power supply 2 5 EPMS 03 The following items are shipped with the EPMS 03 housing Y EPMS 03 cabinet with built in power supply Y Mains cord Y Fuse 034 A 0 2 A slow inserted Front covers DPA 2F npl E d TE PERE Figure 2 Left front view of EPMS 03 housing Right rear panel detail of EPMS 03 and EPMS 07 housing In order to avoid
11. stem The HV TRI50 is designed to inject dyes into cells iontophoretically It can be used in combination with an amplifier module or independently as a iontophoresis system Important For operating the HV TR150 the high voltage version of the EPMS 07 housing is required EPMS H 07 The HV TR150 can deliver max 1250 nA into 100 MQ and can be operated continuously or gated by a TTL signal The injection current can be set either by a built in potentiometer or by a data acquisition system A toggle switch allows convenient switching between recording and injection when operated in combination with an amplifier module using the same electrode This switch can also be operated with a TTL signal Two LEDs at the front panel indicate whether the amplifier or the HV TRI150 is connected to the electrode A red LED at the headstage indicates additionally that the HV TR150 is connected to the electrode A digital display shows the amplitude of the injection current version 1 8 page 6 HV TR150 User Manual 3 4 Description of the Front Panel O HV TR150 npi O CURRENT nA Figure 4 NV TR150 front panel view 1 CURRENT nA display 3 Ya digits display showing the amplitude of the injection current The current that is set with potentiometer 12 or via analog IN connector is always displayed even if actually no current flows because the AMP HV switch 3 is in AMP position This allows first to set the desired current amplitude a
Download Pdf Manuals
Related Search