Leica M165 User's Manual
Contents
1. Parfocality 1 Enlarge the view to the maximum level 2 Focus on the specimen You are done Even if you select a smaller work ing distance the specimen remains pin sharp The parfocality is maintained until you focus on another level of the specimen User Manual lris Diaphragm The iris diaphragm in the optics carrier of your M series microscope has the same purpose as those in a camera shutter it regulates the avail able light which changes the depth of field The depth of field or depth of focus is the area of a specimen that is brought into sharp focus Leica M series Closing the iris diaphragm Close the iris diaphragm by turning the knob to the left The subject appears darker and the depth of field increases Opening the Iris Diaphragm Open the iris diaphragm by turning the knob to the right The subject now appears brighter but the depth of field decreases User Manual Eyepieces Magnification Factor of the Eyepieces An eyepiece not only makes it possible to look passively into the micro scope but also has a critical effect on the maximum magnification The magnification factor is between 10x and 40x The following eyepieces are available for the M series Magnification Dioptric Correction Order number 10x 5 diopter settings 10 450 023 16x 5 diopter settings 10 450 024 25x 5 diopter settings 10 450 025 40x 5 diopter settings 10 450 0262. The instruments and accessories described in this User Manual have been tested for safety and potential hazards The responsible Leica affiliate must be consulted whenever the instrument is altered modified or used in conjunction with non Leica components that are outside of the scope of this manual Unauthorized alterations to the instrument or noncompliant use shall void all rights to any warranty claims as well as product liability and the Declaration of Conformity User Manual Place of use Only use the instruments in closed dust free rooms and between 10 C and 40 C Protect the devices from oil chemi cals and extreme humidity If using the devices outdoors protect them from dust and moisture Never use electrical devices outdoors Electrical components must be placed at least 10 cm away from the wall and away from flammable substances Avoid large temperature fluctuations direct sunlight and vibrations These condi tions can distort micrographic images for example In warm and warm damp climatic zones the individual components require special care in order to prevent the build up of fungus Safety Instructions continued Responsibilities of person in charge of instrument These Safety Instructions must be available at the workplace Ensure that The M series stereomicroscopes and accessories are operated maintained and repaired by authorized and trained person nel only All operators ha
3. mation or explanations that are intended to provide clarity Explanatory notes This symbol within the text stands for addi tional information and explanations Figures 1 Numbers in parentheses within the descriptions relate to the figures and the items within those figures User Manual Notes on how to dispose on the micro scope its components and expend China RoHS 50 year EFUP Environ mentally friendly use period IVD labeling nstrument for in vitro diagnostics wo IVD manufacturing date for example 11 2011 for MM YYYY November 2011 Safety Instructions Description t The individual modules fulfill the highest requirements for observation and docu mentation of Leica stereomicroscopes of the M series Intended use of instrument t Leica Microsystems microscopes are opti cal instruments for improving the visibility of objects or specimens through magnifi cation Accessories such as optical acces sories stands illumination cameras etc supplement the equipment configuration Non intended use Using the instrument in any way contrary to the specifications in the User Manual can lead to bodily harm and damage to objects Never use microscopes for in vivo examina tions or eye surgery if they are not expressly intended for such use Never install any other plug or unscrew optical systems Leica M series and mechanical parts unless expressly instructed to do so in the instructions
4. inserted into the eyepieces 1 Screw the insert off of the eyepiece Leica M series Clamp the graticule on the insert applying moderate pressure Ensure that the grati cule fits tightly Screw the insert and graticule firmly into place and replace the eyepiece in the tube User Manual 4 You can now align the graticule by rotating the eyepiece in the tube and then tighten ing it using the clamping screw 44 Objectives and Optical Accessories The Different Types of Objectives To meet the various requirements regarding imaging properties there is a choice of high quality interchangeable planachromatic and planapochromatic objectives and also lower priced interchangeable achromatic objectives Achromatic objectives are particularly suited for specimens with high contrast structures k Flat field planachromatic objectives are particularly well suited for studying flat objects such as wafers and thin sections With planapochromatic objectives the finest structures are visible with high contrast The sophisticated apochromatic correction allows these objectives to attain the highest color brilliance and fidelity Leica M series Achromatic objectives The 0 32x 0 5x 0 63x 0 8x 1x 1 5x 2x achromatic objectives offer countless variants for selecting the object field diameter magnifi cation ranges and working distances see page 54 Planachromatic objective 1x
5. For the highest requirements for overall image quality we recommend equipping the micro scope with the 1x plan flat field objective which returns sharp contrast rich object fields all the way to the border User Manual Achromatic objectives with a long focal length For special applications achromatic objectives with long working distances and focal lengths of f 100 mm to 400 mm are available 46 Leica M series User Manual 47 Leica TL5000 Ergo Transmitted Light Base Controls 1 2 a gt Rear side of the TL5000 Ergo Left side of the TL5000 Ergo Right side of the TL5000 Ergo 1 A Hu for the Le 1 si display for o ana Ae 1 On Off switch for the light source Press and 2 Power supply connection the aperture or or alance in DF mode hold for5 seconds tovesel the basoto factory 2 Control for the size of the aperture hal default settings nia 2 Controls for controlling the brightness inten 3 BF button for bright field press and si hold for 2 seconds to de activate the automatic aperture 4 RC button for Rottermann Contrast in the RC mode press and hold the button to define the aperture size 5 DF button for dark field Refer also to the separately provided teen O User Manual for the TL5000 Ergo trans mitted light base Leica M series User Manual 48 Leica TL5000 Ergo Transmitted Light Base Operation The transmitted light base must only be connected to a grounded socket
6. Leica TL5000 Ergo Transmitted Light Base 52 Appendix Calculating the Total Magnification and Field of View Diameter 54 Care Maintenance Contact Persons 55 Leica M series User Manual 12 The Leica M Series Leica M series User Manual Congratulations Congratulations on obtaining your new Leica M series stereomicroscope We are convinced it will exceed your expectations as never before have we applied our decades of experience in the areas of optics mechanical engineering and ergonomics in such an uncompromising manner The Leica M series embodies all the qualities you associate with the name Leica Microsystems excellent objectives high quality engineering and reliability Furthermore the modular design ensures that the M series adapts perfectly to your needs no matter which accessories you require for your tasks Leica M series Though the reliability and robustness of Leica stereomicroscopes is legendary like any high tech product the Leica M series requires a certain degree of care and attention Therefore we recommend that you read this manual It contains all the information you need regarding operation safety and maintenance Simply observing a few guidelines will ensure that even after years of intensive use your stereomicroscope will continue to work as smoothly and reliably as on the very first day We wish you the best of success in your work after all you are now equipped with the best tool
7. Use in clean rooms The Leica M series can be used in clean rooms without any problems Cleaning Do not use any unsuitable cleaning agents chemicals or techniques for cleaning Never use chemicals to clean colored surfaces or accessories with rubberized parts This could damage the surfaces and products could be contaminated by abraded particles In most cases we can provide special solu tions on request Some products can be modified and we can offer other accesso ries for use in clean rooms Leica M series The cleaning of glass surfaces and objec tives in particular should be carried out exclusively as outlined in the brochure Cleaning of Microscope Optics The infor mation can be downloaded at http www leica microsystems com prod ucts Select your product and go to the Down load page For additional information refer to page 56 User Manual Servicing Repairs may only be carried out by Leica Microsystems trained service technicians Only original Leica Microsystems spare parts may be used Responsibilities of person in charge of instrument Ensure that the Leica stereomicroscope is operated maintained and repaired by authorized and trained personnel only Important Safety Notes User Manual This User Manual describes the special func tions of the individual modules of the Leica M stereomicroscopy series and contains impor tant instructions for their operational safe
8. User Manual 14 A Step Towards Infinity Ever since their introduction by Horatio S Gree nough stereomicroscopes have worked accord ing to the optical principles based primarily on Ernst Abbe s research For over a century ingenious optics designers and engineers have worked to push magnification resolution and image fidelity to the limit permitted by optics In doing so they have always been constrained by the interrelation between three factors the higher a microscope s resolution the lower the available working distance If one increases the distance of the optical axes the three dimen sional image seen by the observer becomes distorted a sphere becomes an ellipse a flat surface curves towards the observer Leica M series User Manual The Electronics Comfort Convenience and Safety for your Experiments Never before have electronics been used as extensively in a Leica series as in the new M series lineup Optics carrier tripod base and illuminator are all connected using electrical contacts which provides a number of advan tages Contacts not only transmit data but also supply the power Leica M series Fewer cables A large part of the cables have been routed in the interior of the column Data are also trans ferred via the interface between the column the optics carrier and the carrier The direct result is that you need fewer cables this not only makes your workstation neater and more
9. comfortable it even makes it appear larger User Manual Maximum Compatibility Leica engineers were careful to ensure that the new Leica M series like its predecessors remains compatible with existing series This means that objectives bases tubes and so on can be reused Objectives All new objectives of the Leica M series are parfocal If you prefer you can continue to use the previ ous Leica objective series In this case parfocal ity is no longer guaranteed Leica M series Tubes The interface between the optics carrier and the tube has remained the same so exist ing tubes fit the new M series The new tubes are designed for eyepieces with field number 23 while the predecessor models were only designed for field number 21 resulting in a smaller object field Eyepieces The new M series eyepieces have an audible and tangible click to provide immediate feed back in case of accidental adjustment User Manual On We Go If your new Leica microscope has already been assembled and commissioned by your Leica consultant click here to skip through the installation instructions and go directly to the Quick Start Guide on page 28 Leica M series If on the other hand you are assembling the microscope yourself continue with the Assem bly chapter which begins on page 19 User Manual Assembly Leica M series User Manual 19 Base and Focusing Column The first step is to c
10. unsuit able cleaning agents and techniques can damage polymers User Manual Permitted measures Clean the microscope or parts of it using warm soapy water then wipe using distilled water For stubborn dirt you can also use ethanol industrial alcohol or isopropanol When doing so follow the corresponding safety regulations Remove dust with a bellows and a soft paintbrush Clean eyepieces and objectives with special optics cleaning cloths and pure alcohol Copyright Leica Microsystems CMS GmbH Ernst Leitz Strae 35578 Wetzlar Germany 2013 Phone 49 6441 29 0 Fax 49 6441 29 2599 LEICA and the Leica logos are registered trademarks of Leica IR GmbH Printed on chlorine free bleached paper 111 13 M H Revision 1 0 issued March 14th 2013 CE www leica microsystems com eca MICROSYSTEMS
11. with a faultless power cable Failure to observe these warnings may result in serious personal injury or even death The LED illumination can be very bright Check and adjust the intensity of the illumination to a suitable brightness before looking through the eyepieces Leica M series Switching the transmitted light base on and off 2 Press the on off switch on the right side 1 Switch on the transmitted light base with once to turn on the illumination the power switch on the rear side Continued on next page User Manual 49 Leica TL5000 Ergo Transmitted Light Base Operation Continued 3 Look through the eyepiece and adjust the H The following assumes that the power light intensity using the control on the switch on the rear side of the device right side The intensity of the illumination is always switched on This switch is not is visualized by the LED scale mentioned in the remaining part of this User Manual 4 Press the on off switch on the right side once again to turn off the illumination on the base Leica M series User Manual 50 Technical Data Leica TL5000 Ergo Transmitted Light Base Light source Light source LED Illuminated field Bright field 65 mm Illuminated field Dark field Y 40 mm Relief Contrast System RC Yes Internal shutter lamp control Yes Integrated filter holder Yes Matching of high num aperture Yes Remot
12. Leica M series User Manual 40 Health Notes Potential sources of infection Direct contact with eyepieces is a A potential transmission method for bacterial and viral infections of the eye The risk can be kept to a minimum by using individual eyepieces or detachable eyecups Eyecups can be ordered separately Please contact your Leica partner TO LRI ad 43 f 4 76 por 39 Le Gs i la VAL R La CA 1045 ae SCA PT a Separate eyecups are an effective way of prevent ing infections Leica M series User Manual 41 Dioptric Correction All Leica eyepieces are also available with built in dioptric correction allowing the microscope to be used without glasses even by those with vision problems The correction comprises 5 diopter settings Leica M series Using the Dioptric Correction 1 Set the dioptric correction of both eyepieces to the mid position 0 diopter settings While wearing your glasses look through the eyepieces and focus on the specimen Rotate both eyepieces to the maximum value of 5 Hold one eye closed and rotate the other eyepiece in the direction until the speci men appears sharp Then open the other eye and correct the diopter settings until the image is uniformly sharp User Manual Note that when using dioptric correction the advantage of parfocality is lost thus you have to manually refocus each time you change the zoom level To also use parfoc
13. Living up to Life User Manual Leica M125 B Leica M165 C B Seica MICROSYSTEMS CE Copyright All rights to this documentation are held by Leica Microsystems CMS GmbH Reproduction of text or illustrations in whole or in part by print photocopy microfilm or other method including electronic systems is not allowed without express written permission from Leica Microsystems CMS GmbH The instructions contained in the following documentation reflect state of the art tech nology We have compiled the texts and illus trations as accurately as possible Still we are always grateful for comments and suggestions regarding potential mistakes within this docu mentation The information included in this manual may be changed without prior notice Leica M series Revision 1 0 published March 14 2013 by Leica Microsystems Wetzlar GmbH Ernst Leitz StraBe 17 37 D 35578 Wetzlar Germany http www leica microsystems com Responsible for contents Marketing CMS User Manual 2 Function of the Microscopes The Leica M125 B and Leica M165 C B micro scopes for which this User Manual has been written are designed for routine examinations of cell and tissue cultures liquids and sedi ments This includes examining specimens taken from the human body for the purpose of gaining information about physiological or pathological conditions or inborn anomalies or testing for safety and compatibility for poten
14. ality with dioptric correction refer to the instruc tions on page 43 42 Dioptric Correction and Parfocality Leica stereomicroscopes are parfocally matched The prerequisite for this is the correct setting of the diopters and the parfocality The following adjustments have to be carried out only once by each user Preparations k Move the lever of the video phototube to the observation position and open the diaphragm Leica M series Adjusting 1 Set the dioptric correction for both eyepieces to 0 Select the lowest magnification and focus on a flat specimen Select the highest magnification and read just the sharpness Select the lowest magnification again but do not look into the eyepieces Rotate the eyepieces counterclockwise in the direction as far as they will go 5 diopter settings User Manual 7 Look into the eyepieces 8 Slowly rotate each eyepiece individually in the direction until each eye sees the object sharply imaged 9 Select the highest magnification and refo cus if necessary Now if you adjust the magnification from the lowest to the highest level the specimen is always brought into sharp focus If not repeat the process Graticules Use Leica graticules make counting easier particu larly for workstations that are not equipped with a digital camera The Leica graticules for length measurements and numbering are fitted in mounts and are
15. aticule 3 Push the eyepieces into the tubes as far as insert it now page 44 they will go and check to ensure that they Magnification range fit tightly and accurately You can extend the overall magnification range 2 Remove the plastic tube guard using available 10x 16x 25x and 40x wide field eyepieces for persons wearing glasses 4 Securely tighten the clamping screws Leica M series User Manual 23 Objective Tools used No tools required Preparation 1 Remove the protective cap on the optics carrier by turning it Leica M series Attaching the objective Hold the objective firmly during assem bly and disassembly so that it does not fall onto the stage plate This applies particu larly to the 2x planapochromatic objective which is very heavy Remove all specimens from the stage plate first 2 Screw the objective clockwise into the optics carrier User Manual TL5000 Ergo transmitted light base Unpacking the base Focusing drive and column The base is delivered with the adapter plate 1 Unscrew the extension plate from the base installed Make sure the instruments are using the Allen key provided unpacked on a flat sufficiently dimensioned and non slip surface Refer also to the separately provided User Manual for the TL5000 Ergo trans mitted light base Leica M series VOTA ENTES Attach your focusing drive column to the bottom using the 4 Allen screws Reattach
16. e control options Yes AntiShock Pads Yes Dimensions WxHxD 412x341x46 mm Power supply Input 100 240 VAC 47 63 Hz 3 2 A Output 33 VDC 3 93 A 130 W MAX Connections Power supply Leica M series User Manual Appendix Leica M series User Manual 53 Calculating the Total Magnification and Field of View Diameter Parameter Example MO Magnification of objective MO 1x objective ME Magnification of eyepiece ME 25x 9 5 eyepiece Z Magnification changer position Z Zoom position 4 q Tube factor e g 1 5x for coaxial incident light 1 6x for 45 q Coaxial incident light 1 5x tube factor ErgoTube r Factor 1 25x r Factor 1 25x if the planachromatic and planapochromatic objectives of the MZ125 MZ16 are used on the MS5 MZ6 MZ75 or MZ95 NFOV Field number of the eyepiece Field numbers are printed on the eyepieces 10x 21 16x 14 25x 9 5 40x 6 Calculation example magnification in the binocular tube Calculation example field of view diameter in the specimen MTOT VIS MOxMExzxqxr or 1x 25x 4x 1 5 x 1 25 187 5x Leica M series User Manual 54 Care Maintenance Contact Persons We hope you enjoy using your high perfor mance microscope Leica microscopes are renowned for their robustness and long service life Observing the following care and clean ing tips will ensure that even after years and decades your Leica microscop
17. e will continue to work as well as it did on the very first day Warranty benefits The guarantee covers all faults in materials and manufacture It does not however cover damage resulting from careless or improper handling Leica M series EC Declaration of Conformity To download the EC Declaration of Conformity use this link http www leica microsystems com products stereo microscopes macroscopes research manual Select the microscope type and go to the Download page User Manual Care Protect your microscope from moisture fumes and acids and from alkaline caustic and corrosive materials and keep chemicals away from the instruments Plugs optical systems and mechanical parts must not be disassembled or replaced unless doing so is specifically permitted and described in this manual Protect your microscope from oil and grease Do not grease guide surfaces or mechani cal parts Care Maintenance Contact Persons continued Protection from dirt Dust and dirt will affect the quality of your results k Puta dust cover over the microscope when it will not be used for a long time Use dust caps to protect tube openings tubes without eyepieces and eyepieces k Keep accessories in a dust free place when not in use Leica M series Cleaning polymer components Some components are made of polymer or are polymer coated They are therefore pleasant and convenient to handle The use of
18. i ance with this is mandatory Never spill any liquids on electrical instru ments This could cause the stereomicro scope and other equipment to become electrically live and damage people and instruments Never clean instruments using corro sive cleaning agents or those containing acetone For detailed information about care refer to the User Manual for the instru ment Check the power cables regularly Defective power cables can cause injuries Wait for bulbs to cool off before changing them Touching hot bulbs can cause burns Safety Instructions continued Light sources safety regulations External power supply for the focusing External power supply for TL5000 Ergo Light sources pose a potential risk from column Permitted power supply glare Therefore lamps have to be operated Leica M165 C B SINPRO SPU130 110 in closed housings and in installed condi Permitted power supply tion SINPRO SPU46 110 Specifications Input 100 240 V AC k Never look directly into the beam path Specifications 47 63 Hz blinding hazard Input 100 240 V AC 3 2A 47 63 Hz Output 33VDC Do not select a white strongly reflective 1 2A 3 93 A background for the specimen Output 33VDC max 130 W 1 51A For indoor use only For indoor use only Use only the power supply specified A above Other power supplies must not be used If the original power supply fails or is damaged it must be replaced Repair is not permitted Original po
19. onnect the focusing column of the M series to the corresponding base Tools used Allen key 3 mm Leica M series Assembling the column adapter Securely install the column adapter on the column using the four included screws 1 User Manual Assembling the focusing drive 2 Securely screw the focusing column to the base using the six included screws Optics carrier Tools used Assembling the Optics Carrier Allen key 4 mm 1 Place the optics carrier on the focus 2 Press the optics carrier backwards to the ing column so that the screw fits into the focusing column and screw it in place using thread provided and the lug fits into the your other hand groove Leica M series User Manual 21 Tube All intermediate tubes that fit between the Assembling the tube optics carrier and the binocular tube are fitted 2 Push the tube for example the inclined in the same manner binocular tube into the dovetail ring and rotate it slightly in both directions until the Tools used positioning screw meshes with the guide No tools required groove Preparations 1 Unscrew the positioning screw and remove the protective cover Leica M series User Manual While holding the tube only slightly care fully tighten the positioning screw It is automatically brought to the correct posi tion Eyepieces Tools used Preparation Inserting the eyepieces No tools required 1 If you want to use an optional gr
20. ontinued Legal regulations Observe the generally applicable statu tory and country specific regulations for accident prevention and environmental protection EC Declaration of Conformity Electrically operated accessories are constructed based on the state of the art of technology and are provided with an EC Declaration of Conformity See page 55 Health risks Workplaces with stereomicroscopes facilitate and improve the viewing task but they also impose high demands on the eyes and holding muscles of the user Depending on the dura tion of uninterrupted work asthenopia and musculoskeletal problems may occur For this reason appropriate measures for reduction of the workload must be taken Optimal arrangement of workplace work Leica M series assignments and work flow changing tasks frequently Thorough training of the person nel giving consideration to ergonomic and organizational aspects The ergonomic design and construction of the Leica M stereomicroscopy series are intended to reduce the exertion of the user to a minimum Direct contact with eyepieces can be a potential transmission path for bacterial and viral infec tions of the eye The risk can be kept to a minimum by using personal eyepieces for each individual or detachable eyecups User Manual Car eful handling Exercise particular care when setting up the instruments If it is specified that two or more people are required for setup compl
21. sired magnification is configured The rotary knob for the zoom can be used either left or right handed Leica M series User Manual Click Stops and Magnifications The zoom button can optionally be operated Enabling and disabling click stops Magnifications and fields of view either with or without ratchet steps Continu 1 Push the top button downwards to disable The formula on page 54 provides additional ous zoom is possible when the click stops are the ratchet steps information about the magnifications and field disabled which many users find convenient of view diameters with consideration given to On the other hand when the click stops are 2 Push the bottom button upwards to enable the position of the magnification changer and enabled photographs measurement results the ratchet steps the eyepiece and objective combination used etc can be reproduced more accurately Leica M series User Manual 36 Parfocality More Comfort and Convenience for Your Work All Leica stereomicroscopes are parfocally matched meaning that you can view a focused specimen from the lowest to the highest magnification without having to refocus The focus needs to be readjusted only if you want to view a specimen detail that is located higher or lower Requirements for parfocal work If you are using an eyepiece with dioptric correction the procedure differs from this description For more information refer to page 43 Leica M series
22. the adapter plate to its original position using the 6 Allen screws Wiring Connections Leica M165 C B The new Leica M series features extensive encoding with which various microscope data and settings can be read out and reproduced later The interface to the optics carrier is on the column Leica M series The terminals User Manual The connection to other instruments is made using the terminals on the rear side of the column 1 2xCTL2 terminals for auxiliary equipment such as the ring illuminator the base and other accessories from the Leica product range 2 Terminal for the 50 watt power supply provided Cables Cable Duct The integrated cable duct in the column Feeding the cables 3 Place the cables in the cable duct and screw enables a neat cable layout around the micro 1 Unscrew the three screws on the cable the cover on tightly scope For example the USB or FireWire cables duct of the camera can be stowed in the cable duct 2 Remove the cover of the cable duct Tip Estimate the length of the cable ends you will need before screwing on the cover For thick cables it is difficult to change the length retroactively Leica M series User Manual 27 Quick Start Guide The Fastest Route to Success Your Leica stereomicroscope has been delivered in completely assembled condition by your Leica partner and naturally you want to get right to work Therefore your ne
23. tial recipients or for monitoring therapeutic measures IVD The above named microscopes comply with the Council Directive 98 79 EC concerning in vitro diagnostics The manufacturer assumes no liabil A ity for damage caused by or any risks arising from using the microscope for Leica M series other purposes than those for which they are intended or not using them within the speci fications of Leica Microsystems CMS GmbH In such cases the Declaration of Conformity Shall be invalid These IVD instruments are not A intended for use in the patient environ ment defined by DIN VDE 0100 710 Nor are they designed to be combined with medical Leica M125 B nameplate Leica Model M125 B 123456 33 V DC 130W max Leica MicrosystemsCMS GmbH Ernst Leitz Str 17 37 D 35578 Wetzlar 012 IL MOOTO ATA A Dil Ivo CE Lude in Singapore User Manual instruments in accordance with EN 60601 1 Ifa microscope is electrically connected to a medi cal instrument in accordance with 60601 1 the requirements listed in EN 60601 1 1 shall apply Not suitable for examining potentially infec tious specimens This type of instrument may be operated by trained laboratory personnel only Leica M165 CB nameplate la Leica MicrosystemsCMS GmbH ELCOL Emnst Leitz Str 17 37 D 35578 Wetzlar addi 1 20 12 Model Mies ca IMM 33 VDC 1 5 A max OA 33VDC 3 9 A max 1 ME vo CE Made in Srgapore General Notes
24. to this Not to worry after a little while it will become automatic Reference values The distance between eye and eyepiece measures approx 22 mm for 10 23B widefield eyepieces for persons wearing glasses Leica M series User Manual 31 Using the Eyepieces The eyepieces form the connection between the tube and the eye of the observer Simply push them into the tube and they are ready to use Each eyepiece offers a certain magnification factor that has a determinative effect on the total magnification Furthermore all Leica eyepieces can be equipped with practical grati cules that enable measuring and quantifying of specimens Dioptric Correction A built in dioptric correction is available for eyeglass wearers For more information refer to page 42 Leica M series If you do not wear glasses 1 2 Hold the eyepiece firmly and rotate the eyecups forwards counterclockwise If an eyepiece is equipped with the inte If you wear glasses 1 Hold the eyepiece firmly and rotate the eyecups counterclockwise towards the rear as otherwise the viewing distance is too great grated dioptric correction turn the value to the 0 mark User Manual 2 If an eyepiece is equipped with the inte grated dioptric correction turn the value to the 0 mark By the way one benefit of viewing with eyeglasses is a drastically lower risk of bacterial transmission see page 41 The soft material of the eyec
25. ty maintenance and accessories You can combine individual system articles with articles from external suppliers e g cold light sources etc Please read the User Manual and the safety instructions from the supplier Leica M series Before installing operating or using the instru ments read the user manuals listed above In particular please follow all safety instructions To maintain the unit in its original condition and to ensure safe operation the user must follow the instructions and warnings contained in these user manuals User Manual We guarantee the quality of our products Our guarantee covers all faults in materials and manufacture It does not however cover damage resulting from careless or improper handling Symbols Used Warning Safety hazard This symbol indicates especially impor A tant information that is mandatory to read and observe Failure to comply can cause the following Hazards to personnel Functional disturbances or damaged instruments Warning of hazardous electrical voltage This symbol indicates especially impor tant information that is mandatory to read and observe Failure to comply can cause the following Hazards to personnel Functional disturbances or damaged instruments Leica M series Danger due to hot surface A This symbol warns against touching hot surfaces e g those of light bulbs Important information This symbol indicates additional infor
26. up also ensures that your glasses will not be scratched even if they contact the eyepiece Focusing Focusing raises or lowers the stereomicroscope Focusing Coarse fine adjustment using the focusing drive The specimen detail is The inner coarse adjustment is used for The sharpness is adjusted using the coarse fine brought into sharp focus as soon as it is in the covering great distances adjustment focal point of the objective The outer fine adjustment is used for fine 2 The coarse fine adjustment carries a The focusing drive can be operated either focusing load of up to 15 kg left or right handed The resolution of the coarse fine adjust ment is 1 um Leica M series User Manual 33 Adjusting the Resistance of the Focus Drive Adjusting the resistance Is the focusing drive too loose or too tight Does the equipment tend to slide downwards The resistance can be adjusted individually depending on the equipment weight and personal preferences as follows 1 Grip the outer drive knobs with both hands and turn them towards each other until the desired resistance is reached during focus ing Leica M series User Manual 34 Changing the Magnification Zoom All M series microscopes have an integrated Zooming zoom The name indicates the zoom range 1 Look into the eyepieces covered 2 Focus on the specimen Leica M125 B 12 5 1 Leica M165CB 16 5 1 3 Rotate the magnification changer until the de
27. ve read understood and observe this User Manual and particularly the safety regulations Repairs service work Repairs may only be carried out by Leica Microsystems trained service technicians Before opening the instruments switch off the power and unplug the power cable Avoid contact with powered electrical circuits which can lead to injury tapon Use the original packaging for shipping or transporting the individual modules of the Leica M stereomicroscopy series and the accessory components In order to prevent damage from vibrations disassemble all moving parts that accord ing to the user manual can be assembled and disassembled by the customer and pack them separately AE cia in third party products When installing Leica products into third party products the manufacturer of the complete system or its dealer is responsible for following all applicable safety instruc tions laws and guidelines ats Once the product has reached the end of its service life please contact Leica Service or Sales about disposal Please observe and comply with the national and federal laws and regulations that are equivalent to EC directives such as WEEE Like all electronic devices the micro scope its accessory components and Only original Leica Microsystems spare parts may be used consumables must never be disposed of with general household waste User Manual 8 Leica M series Safety Instructions c
28. wer supplies are avail able from your Leica branch office or Leica dealer Leica M series VOTA ENTES 10 Contents Copyright 2 Function of the Microscopes 3 General Notes 4 Important Safety Notes 5 Symbols Used 6 7 Safety Instructions The Leica M Series Congratulations 14 A Step Towards Infinity 15 The Electronics Comfort Convenience and Safety for your Experiments 16 Maximum Compatibility 17 On We Go 18 Base and Focusing Column 20 Optics carrier 21 Tube 22 Eyepieces 23 Objective 24 TL5000 Ergo transmitted light base 25 Wiring Connections Leica M165 C B 26 Cables Cable Duct 27 Leica M series Quick Start Guide The Fastest Route to Success 29 Overview of an M Series Microscope 30 The correct interpupillary distance 31 Using the Eyepieces 32 Focusing 33 Adjusting the Resistance of the Focus Drive 34 Changing the Magnification Zoom 35 Click Stops and Magnifications 36 Parfocality More Comfort and Convenience for Your Work 37 Iris Diaphragm 38 Magnification Factor of the Eyepieces 40 Health Notes 41 Dioptric Correction 42 Dioptric Correction and Parfocality 43 Graticules 44 User Manual 11 Optical Accessories The Different Types of Objectives 46 Bases Leica TL5000 Ergo Transmitted Light Base Controls 48 Leica TL5000 Ergo Transmitted Light Base Operation 49 Technical Data
29. xt step should be to study the Quick Start Guide which outlines the most important steps at a glance This manual will then familiarize you with the finer details of your micro scope The following pages contain important practical information that makes using it every day easier Take time to read it it s worth it MICHORTETES A The M series Quick Start Guide included with instrument Leica M series User Manual Overview of an M Series Microscope Switch click stops on off Magnification changer zoom Coarse fine focusing Transmitted light base Interchangeable objective Built in iris diaphragm Fastening screw for the binocular tube or accessories Adjustable eyepiece tubes Fastening screws for the eyepieces Eyepieces for spectacle wearers with dioptric correction and eyecups 11 Trinocular tube ST DAN ANA da Uy SN Leica M series User Manual The correct interpupillary distance The interpupillary distance is correctly set if you Adjusting the interpupillary distance 3 Slowly approach the eyepieces with your see a single circular image field when looking 1 Look into the eyepieces eyes until you can see the complete image at a specimen field without corner cutting 2 Hold the eyepieces with both hands Push If you are still a novice microscope user you the eyepieces together or separate them may need a short time to become accustomed until you see a circular image
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