Leica DM4000B User's Manual
Contents
1. The display indicates POL Mechanical procedure Turn the polarizer on the underside of the condenser in the light path Fig 66 Make sure that the red index point on the front of the polarizer is aligned with 0 Insert the analyzer into the left side of the stand 67 1 Fig 66 Swivel in polarizer 1 Polarizer Fig 67 1 Bring the polarizer and analyzer into cross po sition until they reach maximum darkness Insert a specimen and rotate a suitable objec tive into place Motorized procedure After selecting the POL contrast method the condenser automatically switches to the posi tion of the polarizer The analyzer cube is also automatically brought into the light path Combined procedure For the Leica DM4000 B and Leica DM5000 B microscopes it is possible to combine mechanical and motorized components Insert analyzer Analyzer 59 8 Imaging Procedure for Leica DM4000 B DM5000 B 8 1 5 Differential Interference Contrast TL Alternatively e Manually rotate the polarizer on the underside AZ MAGRO of the condenser into the light path Fig 66 Switch to the transmitted light axis TL by pushing the TL IL button e Likewise manually insert the analyzer into the left side of the stand Fig 67 Objective and coindenser prisms are automatically moved into the light path as well Insert aspecimen and rotate a suitable objec tive into place Selectthe DIC contrast method2. Caution Cover unoccupied threads on the turret with dust protector caps ni 5 Assembly Fig 9 Fastening the tube 1 Clamping screw Fig 10 Motorized tube connection 1 Connector socket LEICA DM40008 Fig 11 Objective turret with labeled objective receptacles 19 5 Assembly 5 5 Light Sources for the Transmitted Light Axis N Be sure that the lamp housing is discon nected from the power supply Unplug the power plug and the power supply during as sembly Caution 107 2 Lamp Housing This lamp housing is used with a 12V 100W halo gen lamp which is already mounted In case the lamp has to be removed e Remove the fastener screw on the housing Fig 12 e Remove the housing by pulling it upwards e Remove the lamp e Insert the new 12V 100W lamp 13 1 with dust cover straight into the socket until it stops Be sure that the lamp is inserted straight e Remove the lamp s dust cover N Do not remove the lamp s dust cover until you have installed the lamp Avoid fingerprints on the lamp Caution e Replace the housing and fasten it in place us ing the fastening screw e Place the lamp housing in the transmitted light lamp housing receptacle 14 2 and fasten it with the clamping screw on the side e Connect the lamp housing to the power supplyfor transmitted light symbol A 14 3 20 Fig 12 Lamp housing 107 2 Releasing the fastening screw Fig 13
3. 67 14 Index 14 Index Adjusting the light sources 41 Allowable ambient conditions 15 Ambient temperature 8 9 Ambient conditions 15 Analyzer 28 55 56 59 Analyzer cube 55 56 Aperture diaphragm 12 35 38 52 Beam splitting 49 Bright field 53 58 Booster Lens 29 57 Cleaning 64 Cleaning objectives 65 Coaxial pinion 46 Condenser connector 18 Condenser 11 12 18 Condenser centering 38 Condenser height adjuster 17 18 Condenser holder 18 Connection to power supply 30 Dark field 54 58 Dark field stop 54 Defined function keys 36 DICprisms 28 Differentiated interference contrast 56 Display 12 35 DMControl 11 14 Electrical safety 8 Electronics box Leica CTR5000 8 30 Ergomodule 29 Excitation Manager 10 29 57 Eyepiece 12 49 Field diaphragm 12 35 52 Filter block exchanger 13 Filter cube 26 Filter cube ICR 59 60 FIM Fluo Intensity Manager 10 52 68 Fluorescence 57 Fluorescence intensity 57 Focus finder 33 Focusing 37 47 Focusing telescope 39 Function keys 12 31 36 Gas discharge lamps 23 24 Halogen lamp 21 22 42 Hg 50 burner 24 ICT P transmitted light polarizator 27 Identification Sheet 19 26 28 53 Imaging procedure10 53 58 Immersion oil 50 65 Incident light axis 10 Incident light nosepiece disc 26 Incident light polarizators 27 Incident light shutter 35 Initialization 34 Interference contrast 60 Intermediate systems 16 K hler illumination 18 37
4. Lamp housing 107 2 opened 1 Mount with halogen lamp 2 Collector Fig 14 Rear side of stand 1 Incident light lamp housing receptacle 2 Transmitted light lamp housing receptacle 3 12V 100 W connection for transmitted light symbol J 4 12V 100 W connection for incident light symbol 7 5 6 Light Sources for the Incident Light Axis Caution During assembly always unplug the power supply unit of the 106 z lamp housing from its socket Never touch the glass parts of the burner with bare hands Never look directly into the beam path blind ing hazard During assembly work on xenon burners al ways wear the supplied protective gloves and face protection Fig 15 risk of explosion 106 z lamp housing Fig 15 Protective gloves and mask Si 5 Assembly This lamp housing is used with a 12V 100W halo gen lamp or various gas discharge lamps Inserting the 12V 100W halogen lamp into the 106 z lamp housing e Unscrew the fastening screws of the cover and lift up the cover 16 1 e Unscrew the fastening screws of the lamp mount 16 8 and pull out the mount Fig 17 Fig 16 106 z lamp housing on the side open 1 Cover raised 2 Collector 3 12 V 100 W lamp or gas discharge lamp in mount 4 Reflector mirror 5 6 7 Adjusting screw for x y reflector 8 Fastening screw for lamp mount 9 Socket for contact plug 21 5 Assembly e Insert the lamp with the dust cover straight i
5. increments p 52 Light Intensity N a 1 N The values for the field diaphragm FD and the aperture diaphragm AP are indicated numeri cally The field diaphragm may be either round or rectangular Accordingly the FD designation is setin parentheses or in brackets FD or FD A When using a digital camera rectangular field diaphragms are recommended Diaphragms Note ro If a motorized tube is used the light splitting between ocular Eye and photo output Docu is indicated in A The display may flash after the initialization phase or even during microscopy session This always occurs when the contrast method selected can not be performed with the actual microscopic settings For example an objective may be swiveled in that is not suited to the contrast method chosen Then check your settings Beam splitting Note 35 6 Startup There is a row of function keys both on the right and left side of the stand Some of these keys are defined and some of them are variable The variable function keys have various meanings depending on the microscope configuration Defined Function Keys on the left side of the stand The TL IL key 38 1 switches between incident light and transmitted light The last contrast method used is restored The INT 38 3 keys adjust the light intensity indi vidually Settings can be made either in large or small increments Pushing both INT buttons at the
6. light ICT Pol e incidentlight BF DF ICR Pol automatic Illumination Manager motorized aperture diaphragm and field diaphragm motorized intensity control automatic Constant Color Intensity Control CCIC motorized shutter integrated into the stand motorized 5x filter turret DM5000 B 8x optional with FIM Fluorescence Intensity Managemer for de creasing light intensity in 5 stages mechanical Booster Lens for increasing fluorescence intensity e motorized shutter integrated into the stand motorized 4x filter turret automatic Illumination Manager motorized shutter Objective nosepiece X Y Stage manual absolute coded 6x with M25 thread DM5000 B 7x mot DIC objective prism turret with 4 positions optional manual replaceable specimen stage coaxial pinion length 155 mm e manual or motorized manual absolute encoded 6x with M32 thread slot for DIC prisms and Pol compensators optional manual replaceable specimen stage coaxial pinion length 140 mm e optionally with two camera outputs 3 Overview of the Instrument Leica D000 B DMS000 8 Leica Manmmm Condenser e motorized condenser head e motorized condenser turret for light rings DF stop DIC prisms e optional polarizer integrated and motorized e automatic K hler Illumination Magnification Changer manual e manual e absolute coded e absolute coded e 1x 1 25x 1 6x e 1x 1 5x 2x Control Panels operat
7. High pressure xenon burner direct current stabilized 400 hrs Please regard the data sheets of the burners y 23 5 Assembly e To open the 106 z lamp housing unscrew the fastening screws on the cover Caution e Remove the transport anchorage red plastic Hg 50 burner rod in place of the burner in the lamp mount After installation the labeling must be upright To do so remove the lower clamp 19 1 Pull fa glass melt nipple is present 19a 4 posi up the cooling element 19 3 and turn itto the tion it by turning the burner so that the nipple side Detach the lower clamp system 19 2 does not come in the way of the beam path and remove the transport anchorage later but instead is positioned sideways e Install the burner in mirror image fashion Xe 75 burner Remove the burner s dust cover 19b 5 after you have installed the burner Fig 19 a d Lamp mounts for gas discharge lamps 1 Upper clamping system 2 Lower clamping system 3 Cooling element 4 Nipple of the mercury 50 burner 5 Dust cover of the mercury 75 burner 24 e Insert the lamp mount with the burner in stalled into the lamp housing and tighten it with the screws 20 8 e Put the lid down again Plug in the contact plug as far as it goes and retighten the screws e Place the lamp housing in the incident light lamp housing receptacle 21 1 and fasten it with the clamping screw on the side Connect the lamp housing to the power supp
8. Lamp housing 13 Lamp housing 106 z 21 41 Lamp housing 107 2 20 41 Lamp housing receptacle 20 22 25 Light intensity 35 Light sources 41 52 Light sources incident light axis 21 Light sources transmitted light axis 20 Magnification changer 11 51 Mercury lamp Hg 50W 43 Mercury lamp Hg 100W Xe 75W 44 Mirror housing 29 Motorized analyzer 28 Motorized polarizer 27 Objective aperture 54 Objective prism slide 60 Objectives 19 50 Objective turret 10 12 Object stage 17 46 Phase contrast 39 53 Phase contrast rings checking 39 Polarization 55 59 Polarizer 55 56 59 Polarizer L ICR 27 Polarizer R ICR 27 Polarizer R P 27 Prism slot 60 Reflector cube 26 Reflector cube for lamp adjustment 41 Reset function 31 Retention pin 26 Rotating polarizer 27 Shutter 57 Software Leica DMControl 11 14 Specimen holder 17 Supply unit ebq 100 9 25 46 Switch transmitted incident light 12 Touchscreen 11 Transmitted light and incident light analyzer 28 Transmitted light axis 10 Transmitted light filter 13 Transmitted light shutter 35 Variable function keys 12 13 31 36 Vision problems 49 Xe 75 burner 24 15 EU Declaration of Conformity 15 EU Declaration of Gonformity
9. lie slightly outside the center Fig 54 Then pivot the arc s mirror image with the ad justing knobs 46 2 and 46 4 and focus it us ing the reflector 46 3 Use the adjusting knobs 46 2 and 4 to orient the mirror image symmetrically to the direct image Fig 55 The V shaped irradiation of the direct image and mirror image arcs can be superimposed Caution The bright tips of the arcs the focal spots must never be projected onto each other as this re sults in a danger of explosion by overheating 44 Fig 53 Direct arc image focused but not centered in reality the image is less focused Fig 54 Direct arc image in target position in reality the image is less focused Fig 55 Direct arc image and mirror image in target position in reality the image is less focused In older lamps the structure of the arc is no longer clearly recognizable The image is then more like that of a HG 50 lamp The im age and mirror image can no longer be su perimposed exactly In this case align both images e Using the collector defocus the image with the knob 46 6 until the arc image and mirror image are no longer recognizable and the im age is uniformly illuminated Exchange the reflector cube for lamp adjustment for the original filter cube Note Turn off the microscope before exchanging the reflector cube 6 Startup 45 7 Operation 7 Operation 7 1 Switching on the Microscope
10. not turn on the power supply unit yet First work in transmitted light in order to familiarize yourself with the microscope s controls 6 3 The Display Leica DM4000 B DM4000 M Fig 37 Display after initialization The display shows the current microscope set tings The display depends on the microscope s configuration In the first column corresponding pictograms indicate the type of information con trast method magnification light intensity dia phragms light splitting for photo tubes Please see the abbreviation index for a list of ab breviations and pictograms used p 67 Contrast Method n the first row you find an indication of the ac tive light axis transmitted light or incident light of the current contrast method and the current filter cube The shutter status is displayed for the transmitted light or incident light shutter Transmitted light shutter open Transmitted light shutter closed Incident light shutter open Incident light shutter closed Ad Magnification E current objective magnification sometimes followed by the re magnification of the magnifi cation changer appears along with the total magnification 2 Objective x Re magnification x Eyepiece 6 4 The Function Keys ll 6 Startup N La 1 N The actual brightness setting is graphically de picted by a beam Additionally the light intensity is indicated in 20 coarse adjustment or in 255 fine adjustment
11. that the centering surface is halfway covered Fig 48 Then pivot the lamp filament s mirror image with the adjusting knobs 46 2 and 4 and focus it using the reflector 46 3 Align the mirror image symmetrically to the fil ament image Fig 49 To do so use the adjust ing knobs 46 2 and 46 4 again Defocus the image with the collector head 46 6 until the filament image and mirror im age are no longer recognizable and the image is uniformly illuminated Exchange the reflector cube for lamp adjustment for the original filter cube Note Turn off the microscope before exchanging the reflector cube Centering the Hg 50 W mercury lamp 42 In the adjustment window you see the direct arc image and the mirror image which in most cases are shifted together Fig 47 Directlamp filament image focused but not centered in reality the image is less focused Fig 48 Direct lamp filament image in target position in reality the image is less focused Fig 49 Direct lamp filament image and mirror image in target position in reality the image is less focused IN Focus the direct image with the collector 46 6 Use the adjusting buttons on the rear side of the lamp housing 46 2 46 4 to pivot the arc s mirror image to the side or completely out of the beam path The lamp filament s focused image remains visible Fig 50 Use the adjusting buttons 46 1 and 46 5 to place the direct arc image
12. to the documentation tube BDT25 but itis motorized The control positions are selected using a vari able function key on the stand HC L 2TU tube The beam splitting is set manually by pulling out a control bar Control Bar Observation Photo VIS C 100 0 PHOTO e 0 100 Fig 62 BDT25 tube with digital camera 1 Control bar Me 7 Operation 7 5 Eyepieces A The eyepiece s aperture protector must be removed or at least folded back during microscopy while wearing eyeglasses Eyeglasses with multifocal lenses bifocals and smooth view glasses must be removed while operating the microscope Note e For the adjustable tubes with documentation output choose the 100 position Eyepieces with Inlaid Reticle e Focus the reticle by adjusting the eyelens e Focus on the object through this eyepiece e Then close that eye and focus on the specimen by adjusting only the second ocular Correction for Vision Problems e With your right eye look through the right eyepiece and bring the specimen into sharp focus e Then with your left eye view the same speci men and rotate the left eyepiece tube until the object is brought into sharp focus Do not use the focus dial 49 7 Operation 7 6 Objectives The objective must be moved manually into the light path Be sure that the nosepiece turret locks into place The objective s position in the turret is factory set and must be adhered to while screw
13. 3 4 Unpacking the Microscope 4 Unpacking the Microscope The device is delivered in two boxes The stand box contains the following compo nents Stand with integrated incident light axis and objective nosepiece Specimen stage with stage bracket Power cable and PC connecting cable e CD with Leica software package Instructions and list of microscope default settings Identification Sheet The system box contains the microscope acces sories e Tube e Eyepieces Objectives e Condenser e Lamp housings with accessories Fitting tool Depending on configuration additional micro scope accessories such as filter cubes etc The external ebg 100 supply unit is delivered in separate packaging For the Leica DM5000 B microscope The CTR5000 electronics box is also delivered in separate packaging First carefully remove all components from the transportation and packaging materials A Avoid touching the lens surfaces of the objectives If fingerprints do appear on the glass surfaces remove them with a soft leather or linen cloth Even small traces of finger perspiration can damage the surfaces of optical surfaces in a short time See the chapter Care of the microscope p 64 for additional in structions N Do not yet connect the microscope and pe ripherals to the power supply at this point Note Caution Installation location Work with the microscope should be perfo
14. Do so by pressing the DIC variable key Alternatively Press the CHANGE TL variable key For key occupation please see Identification Sheet The display indicates ICT e Fine adjustment is possible using the knurled ring above the objective nosepiece The polarizer located in the condenser and the fitting condenser prism are automatically brought into the light path The corresponding objective prism and the analyzer cube are also positioned automatically For fine adjustment use the knurled ring above the objective nose piece Fig 68 Fig 68 Objective prism slide 1 Knurled wheel for fine adjusting 56 8 Imaging Procedure for Leica DM4000 B DM5000 B 8 2 Fluorescence Fig 69 Switch to the fluorescent light axis FLUO by pushing the TL IL button Insert a specimen and rotate a suitable objec tive into place The current fluorescence cube is indicated on the display Closing the incident light shutter protects your specimen from fading Do so by pressing the SHUTTER variable key For key occupation please see Identification Sheet The display indicates the symbol X4 Selecting the fluorescence filter cube Press the variable keys Cube U or Cube VD Inserting the Booster Lens Fig 70 The fluorescence intensity can be increased using the Booster Lens on the right side of the stand Fig 69 For multifluorescence use of a Excitation Manager is recommended The Excitation Man
15. I CHANGE COMBI O 32 Meaning Bright field Transmitted light Phase contrast Transmitted light Interference contrast Transmitted light Dark field Transmitted light Polarization Transmitted light Switch through all transmitted light processes Increase brightness transmitted light Reduce brightness transmitted light Open aperture diaphragm transmitted light Close aperture diaphragm transmitted light Open field diaphragm transmitted light Close field diaphragm transmitted light Open close transmitted light shutter Fluorescence last filter cube Select fluorescence cube at position 1 Switch through fluorescence cubes in clockwise fashion Switch through fluorescence cubes in counterclockwise fashion Open close fluorescence shutter Increase brightness fluorescence Reduce brightness fluorescence Open field diaphragm fluorescence Open field diaphragm fluorescence Combination mode PH fluorescence or ICT fluorescence Switch through all combination modes For Leica DM4000 M Function key BF ICR DF POL CHANGE RL 9 SHUTTER RL FLUO CUBE 1 CHANGE FLUO FOCUS FINDER BFTL INT T INT J AP 7 AP J FD T FD J COMBI O RD 6 Startup Meaning Bright field Incident light Interference contrast Incident light Dark field Incident light Polarization Incident light Switch through all incident light processes Increase brightness incident light Reduce br
16. Leica DM4000 B Leica DM4000 M Leica DM5000 B Operating Manual MICROSYSTEMS Published 2003 by Leica Microsystems Wetzlar GmbH Ernst Leitz Stra e D 35578 Wetzlar Germany Responsible for contents Katja Peter Karin Schwab Marketing CM Compound Microscopy Product Management Phone Fax In case of questions please contact E mail 49 0 6441 292261 49 0 6441 292255 MOM Hotline leica microsystems com Leica DM4000 B Leica DM4000 M Leica DM5000 B Operating Manual MICROSYSTEMS Copyrights Copyrights All rights to this documentation are held by Leica Microsystems Wetzlar GmbH Reproduction of text or illustrations in whole or in part by print photocopy microfilm or other methods includ ing electronic systems is not allowed without express written permission from Leica Microsystems Wetzlar GmbH The term Windows can be used in the following text without further identification It is a registered trademark of the Microsoft Corporation Otherwise no inference with regard to the free usability of product names may be drawn from the use of those names The instructions contained in the following docu mentation reflect state of the art techno logy and knowledge standards We have compiled the texts and illustrations as accurately as possible Nevertheless no liability of any kind may be assumed for the accuracy of this manu al s contents Still we are always grateful for comments and sugge
17. Leica DM4000 B Leica DM5000 B 8 1 Transmitted Light 8 1 1 Bright Field TL Switch to the transmitted light axis TL by pushing the TL IL button Select the BF bright field contrast method Do so by pressing the BF variable key Alternatively Press the CHANGE TL 9 variable key For key occupation please see Identification Sheet The display indicates BF Insert a transmitted light specimen Rotate an appropriate objective into place Bring the image into focus using the focus dial and set the brightness using the INT function key 8 1 2 Phase Contrast Switch to the transmitted light axis TL by pushing the TL IL button Select the PH contrast phase contrast method Do so by pressing the PH variable key Alternatively Press the CHANGE TL variable key For key occupation please see Identification Sheet The display indicates PH Insert a transmitted light specimen Rotate an appropriate objective into place Objectives that are suitable for phase contrast are engraved with PH Bring the image into focus using the focus dial and set the brightness using the INT function key 03 8 Imaging Procedure for Leica DM4000 B DM5000 B 54 A Notes The microscope automatically selects the correct light ring in the condenser When selecting the phase contrast method the aperture diaphragm is opened completely and may not be adjusted To avoid errors in operation the function keys f
18. W High pressure mercury burner 100 W 103 W 2 High pressure xenon burner 75 W Screw cap for unused objective receptacles 020 422 570 000 Screw cap M 25 Replacement eyecup diaphragm protection for HC PLAN eyepiece 021 500 017 005 021 264 520 018 021 264 520 018 HC PLAN eyecup HC PLAN eyecup HC PLAN eyecup Immersion Oil conforming to DIN ISO standards fluorescence free 513 787 513 522 513 788 10 ml 100 ml 500 ml Used for 107 2 lamp housing 106 z lamp housing 106 z lamp housing 106 z lamp housing 106 z lamp housing Objective turret 10x 25 eyepiece 10x 22 eyepiece 10x 20 eyepiece OIL and IMM objectives and oil condenser heads 13 Abbreviations and Pictograms 13 Abbreviations and Pictograms o oe Ke A AET BF COMBI CUBE DF DIC FD FLUO ICR ICT INT MBDT PH POL TL Contrast method Magnification Light intensity diaphragms Beam splitting Transmitted light shutter open Transmitted light shutter closed Incident light shutter open Incident light shutter closed Advanced Ergo Tube Aperture diaphragm Brifght field Combination contrast method Filter cube Darkfield Differentiated interference contrast Field diaphragm Fluo axis incident light Interference contrast reflected light Interference contrast transmitted light Incident light axis Intensity Motorized Basic Documentation Tube Phase contrast Polarization Transmitted light axis
19. When using a gas discharge lamp the ebq 100 external supply unit must be turned on separately 56 1 Then switch on the microscope at the power switch All motorized microscope components first un dergo an initialization phase After the initialization is complete the display on the stand Fig 57 shows the current microscope setting Fig 56 Front view of the ebg 100 supply unit 1 Power switch 2 Lamp status Fig 57 Display after initialization 46 7 2 Stages and Specimen Displacement Lengthening the coaxial pinion e For lengthening pull the lower grip 58 2 downwards Repeat with the upper grip 58 1 Torque adjustment The torque is already optimally set at the factory however it can be individually adjusted using two knurled rings 58 3 58 4 Fig 58 Revolving object stage Object motion Y direction Object motion X direction Torque adjustment Y direction Torque adjustment X direction Focus dial for fine focusing 1 2 3 4 5 Rotating the Stage The swiveling range of the rotating stages Is 0 110 e In order to revolve the stage loosen the fas tening screw 59 1 e Bring the table into the desired position e Retighten the fastening screw Me 7 Operation 7 3 Focusing There is a focus dial on the left side of the stage for coarse and fine focus adjustment Fig 59 On the right side of the stand there is also a focus dial which is used exclusively for fine
20. aces with a fine dry and fat free hair brush by blowing with a blow bag or vacuum suction Carefully remove stubborn dirt on glass surfaces with a clean cloth moistened with distilled water If the dirt still can not be removed use pure alcohol chloroform or benzine Cleaning Objectives A The objective may not be unscrewed during cleaning If damage appears on inner sur faces the objectives must be sent to your Leica subsidiary for repair We also advise against cleaning the inside surfaces of the eyepieces Caution The front lenses of objectives are cleaned as de scribed under Cleaning Glass Surfaces The upper lens is cleaned by being blown off with a pneumatic pump 11 Care of the Microscope Removing Immersion Oil N Follow safety instructions for immersion oil Caution First wipe off the immersion oil with a clean cot ton cloth and then re wipe the surface several times with ethyl alcohol 11 3 Handling Acids and Bases For examinations using acids or other aggres sive chemicals particular caution must be taken Caution Be absolutely certain to prevent the optics and mechanical parts from coming these chemicals 65 12 Wear and Spare Parts 12 Essential Wear and Spare Parts Replacement Lamp 66 Order No Material No 500 974 500 137 500 138 500 321 500 139 Name Halogen lamp 12 V 100 W High pressure mercury burner 50W High pressure mercury burner 100
21. afe operation the user must follow the Ambient temperature 15 35 C instructions and warnings contained in this operating manual N The devices and accessories described in this operating manual have been tested for safety and potential hazards The responsible Leica affiliate or the main plant in Wetzlar must be consulted when ever the device is altered modified or used in conjunction with non Leica components that are outside of the scope of this manual Caution Unauthorized alterations to the device or noncompliant use shall void all rights to any warranty claims Relative humidity Overvoltage category Pollution degree Microscope For indoor use only Supply voltage Frequency Power input DM4000 DM5000 Fuses DM4000 DM5000 Ambient temperature Relative humidity Overvoltage category Pollution degree max 80 to 30 C Il 2 90 250 V 50 60 Hz max 180 VA max 290 VA T6 3 A IEC 60127 2 3 See CTR5000 15 35 C max 80 to 30 C Il 2 2 Safety Notes Supply unit ebq 100 Caution For indoor use only Supply voltage 90 250 V Frequency 50 60 Hz Never use any fuses as replacements other Power input max 155 VA than those of the types and the current rat Pikes 2xT2A IEC 127 ings listed here Using patched fuses or Ambient temperature 15 35 C bridging the fuse holder is not permitted Relative humidity max 80 to 30 C Overvoltage category Il Pollut
22. ager is inserted into the right side of the stand up to the last stop Fig 70 Using Booster Lens and Excitation Manager the Excitation Manager can be inserted into the back receptacle Inserting the Excitation Manager 57 9 Imaging Procedure for Leica DM4000 M 9 Imaging procedure for Leica DM4000 M 9 1 Incident Light 9 1 1 Bright Field e Switch to the incident light axis IL by pushing the TL IL button Select the BF bright field contrast method Do so by pressing the BF variable key Alternatively Press the CHANGE RL variable key For key occupation please see Identification Sheet The display indicates BF Insert a specimen Rotate an appropriate objective into place e Bring the image into focus using the focus dial and set the brightness using the INT function key 08 9 1 2 Dark Field Switch to the incident light axis IL by pushing the TL IL button Select the DF dark field contrast method Do so by pressing the DF variable key Alternatively Press the CHANGE RL variable key For key occupation please see Identification Sheet The display indicates DF The DF reflector is turned into the beam path Insert a specimen Rotate an appropriate objective into place Bring the image into focus using the focus dial and set the brightness using the INT function key A Notes The maximum objective aperture which may be used for dark field is 0 75 All o
23. bjectives with greater aperture are automatically blocked for this procedure DF flashes in the display When selecting the dark field method the aperture diaphragm is opened completely and may not be adjusted To avoid errors in opera tion the function keys for setting the aperture diaphragm AP are locked 9 1 3 Polarization Fig 71 1 2 3 Switch to the incident light axis IL by pushing the TL IL button Select the POL polarization contrast method Do so by pressing the POL variable key Alternatively Press the CHANGE RL 9 variable key For key occupation please see Identification Sheet The display indicates POL Objective prism slide Knurled wheel for fine focusing Prism slot with inserted objective prism slide Insert polarizer 9 Imaging Procedure for Leica DM4000 M Automatic procedure The ICR filter cube is automatically brought into the light path Mechanical procedure Rotate the appropriate polarizer 71 3 and the IC P analyzer 72 1 on the stand manually into the light path Also bring the polarizer and analyzer into cross position until they reach maximum darkness Insert a specimen and rotate a suitable objec tive into place Fig 72 Insert analyzer 1 59 9 Imaging Procedure for Leica DM4000 M 9 1 4 Interference Contrast Switch to the incident light axis IL by pushing the TL IL button Insert a specimen and rotate a suitable objec tive int
24. cation Fig 64 Immersion objective locked 51 1 7 8 Light Sources The brightness is set using the function keys 65 5 Then the INT function keys are as signed to the currently active axis for trans mitted light TL or incident light IL For TL and IL Settings can be made either in large or small increments Pushing both INT buttons simultaneously switches between coarse and fine setting The display indicator changes ac cordingly 0 20 Coarse adjustment u 0 255 Fine adjustment PE For Fluo The brightness is set in 5 fixed steps FIM 100 55 35 20 10 Fig 645 Control panel Variable function keys Aperture diaphragm Transmitted light incident light Field diaphragm Light intensity CI SBN 52 Operation 7 9 Aperture Diaphragm and Field Diaphragm Both diaphragms are already factory set to the optimum setting for the current objective e The AP 65 2 keys for the aperture diaphragm and the FD keys 65 4 for the field diaphragm may be used to change each diaphragm s set ting at any time Then the function keys are assigned to the currently active axis for transmitted light TL or incident light IL A Caution When doing so old values are overwritten and the new values are stored A Caution While using PH or DF the aperture diaphragm is completely opened and locked 6 Imaging Procedure 8 Imaging Procedure for Leica DM4000 B DM5000 B for
25. cident light analyzer 2 5 Assembly e Remove the plug cap on the left side of the Insert the objective prism into the tube slot stand Fig 31 1 The code letter must match the code letter on the objective e Insert the polarizer into the receptacle until it latches in place Fig 30 e With the microscope Leica DM5000 B the DIC objective prisms are already mounted in the DIC turret above the objective revolving Motorized analyzer nosepiece Fig 68 e Insert the analyzer cube as described in sec tion 5 7 Equipping the Incident Light filter turret p 26 in the corresponding position on the filter turret See the list provided identification Sheet for the correct position 6 9 DIC Prisms 5 10 Optional Accessories Fig 30 Inserting the analyzer Fig 31 Inserting the objective prism slide 1 The plug cap is replaced with the analyzer 1 Objective prism slide 28 Ergomodule For raising the eye level ofthe tube opening the ergomodule may be used It is fastened in place with the side clamping screw Mirror Housing Place the mirror housing directly onto the lamp housing receptacle on the back of the stand and attach it using the side clamping screw Place the lamp housing onto the mirror hous ing and fasten it using the corresponding clamping screw on the side Booster Lens Excitation Manager Fig 32 1 Insert of Booster Lens SS 5 Assembly Remove the clamping ring from
26. denser e Tube e Eyepieces e Objectives e Light sources Filter cubes reflectors Only a few commonly used screwdrivers and keys are necessary for assembly which are in cluded in the delivery package 5 1 Stage e Caution Before assembling the stage make sure no ob jectives are installed e Place the specimen holder on the stage and fasten it with the two screws 3 1 e Using the condenser height adjuster 3 2 turn the condenser holder completely upwards i e as close to the stage as possible e Loosen the stage clamp 3 3 slightly Fig 3 Mechanical object stage 1 Locking screws for specimen holder 2 Condenser height adjuster 3 Stage clamp Si 5 Assembly e From above set the stage clamp onto the dovetail guide 4 2 and push the stage down wards until the upper end of the dovetail guide is tightly fastened to the upper end of the stage clamp e Firmly tighten the stage clamp 4 1 A Note For thicker specimens Leica DM4000 M the stage can be set to a correspondingly lower level Fig 4 Assembling the stage 1 Stage clamp 2 Dovetail guide 17 5 Assembly 5 2 Condenser Fig 5 1 2 3 4 Using the condenser height adjuster 5 4 turn the condenser holder 5 1 completely down wards Unscrew the clamping screw for the con denser 5 3 far enough so that the condenser can be inserted from the front From the front insert the condenser into the condenser holder a
27. e on the right side and press it to the left into the mounting Fig 26 e Push the retention pin 25 2 and continue to turn the filter turret until you reach the next locking position e Again make sure that the turret engages retention pin unlocks and insert the next filter and or reflector cube as described Fig 26 Inserting the filter or reflector cubes above 1 Mounting e When all filters and reflector cubes have been inserted close the front cover plate again 5 8 Polarizer and Analyzer 26 5 Assembly ICT P transmitted light polarizer Fig 27 Assembly of the ICT P transmitted light polarizer 1 Clamping screw Using the left clamping screw fasten the ICT P transmitted light polarizer to the underside of the condenser holder Fig 27 Make sure thatthe red index point on the front of the polarizer is aligned with 0 If necessary insert the compensators A and M4 plates into the polarizer s receptacle Fig 28 Fig 28 Inserting the compensators Incident light polarizers R P polarizer rotating polarizer L ICR R ICR polarizer Remove the plug cap on the right side of the incident light axis Fig 29 Fig 29 Inserting the polarizer 1 The plug cap is replaced with the polarizer Insert the polarizer into the receptacle until it latches in place Motorized polarizer A motorized polarizer is already installed and ready for operation in the DIC condenser Transmitted light and in
28. ering Be sure that a DF objective is being used gt The objective aperture setting is too high maximum 0 75 If necessary reduce the ob jective aperture using the iris diaphragm on the objective Check the condenser centering The magnification is too weak Use a higher magnification Clean the specimen and neighboring lenses gt p 65 The specimen is too thick gt The refraction index of embedding material and object is identical The cover glass is not placed evenly Therefore check the centering of the light rings gt p 39 Problem Polarization No polarization contrast is possible Fluorescence The image is completely dark no fluorescence The fluorescence is too weak Display The display flashes FAIL appears eg 10 Trouble Shooting Cause Remedy gt Bring the polarizer and analyzer into cross po sition until they reach maximum darkness without specimen p 55 59 Open the shutter p 57 Select the incident light axis IL p 36 Check the antigen antibody combination Insert the Booster Lens p 29 Center the lamp p 41ff Insert a new lamp p 20f Rotate an appropriate objective for the con trast method into the light path Check insertion of objectives cubes etc 63 11 Care ofthe Microscope 11 Care of the Microscope Caution N Unplug the power supply before perfor
29. focusing 58 4 The special design of this dial makes it possible to simultaneously grasp the coaxial drive with your hand while operating the fine drive with one finger Fig 59 Revolving object stage 1 Clamping screw 2 Fine focusing 3 Coarse focusing 4 7 Operation 7 4 Tubes A Close any unused tube openings as otherwise stray light can interfere with observation A Make sure that the connector cable is plugged in on the MBDT25 motorized tube 60 1 Note Note Adjusting the Viewing Distance e Adjust the viewing distance of the eye pieces so that a congruent total image Is seen Fig 60 Fig 60 Tube setting gt Personal eyebase settings 1 Motorized tube connection 48 Adjusting the Viewing Angle e For the AET22 and EDT22 ergotubes the view ing angle can be adjusted by tilting the binocu lar viewer in the range of 5 32 Fig 61 Adjusting the Eyepiece Extension to the Arm Length e With the AET22 tube the eyepieces can be extended up to 30 mm Fig 61 Fig 61 With AET22 tube individual adjustments E E E S q Beam Splitting in Photo Tubes EDT22 tube The beam splitting between the observation and documentation outputs has a definite presetting 50 50 BDT25 tube The beam splitting is set manually by pulling out a control bar Control Bar Observation Photo VIS LE 100 0 50 50 LE 50 50 PHOTO 0 100 MBDT25 tube This tube is similar
30. hanged filter cube is shown in the upper right of the display N Never look directly into the light path Caution Fig 45 Reflector cube for lamp adjustment similar to illustration 6 Startup When switching to the BF or Smith reflectors there is a danger of being glared For the 106z lamp housing the direct filament im age for halogen lamps or direct arc image for gas discharge lamps and its mirror image are focused separately and adjusted to each other On the left side of the microscope there is an adjustment window 1 14 p 12 for mapping the light source While observing the light source in the adjust ment window the lamp is adjusted as follows Centering the 12 V 100 W Halogen Lamp Fig 46 106z lamp housing 1 Lamp height adjustment 2 4 Mirror image height and side adjustment 3 Focusing the reflector 5 Lamp side adjustment 6 Collector focusing of the lamp image 41 6 Startup In the adjustment window you see the direct filament image and the mirror image which in most cases are shifted together Focus the direct filament image with the col lector 46 6 Use the adjusting buttons on the rear side of the lamp housing 46 2 46 4 to pivot the lamp filament s mirror image to the side or com pletely out of the beam path The lamp fila ment s focused image remains visible Fig 47 Adjust the direct filament image using the ad justing knobs 46 1 and 46 5 so
31. ightness incident light Open aperture diaphragm incident light Close aperture diaphragm incident light Open field diaphragm incident light Close field diaphragm incident light Open close incident light shutter Fluorescence last filter cube Select fluorescence cube at position 1 Switch through fluorescence cubes Select smallest field diaphragm and switch back to original field diaphragm by pressing the key again Bright field Transmitted light Increase brightness transmitted light Reduce brightness transmitted light Open aperture diaphragm transmitted light Close aperture diaphragm transmitted light Open field diaphragm transmitted light Close field diaphragm transmitted light Combination process BF and BF TL 33 6 Startup e Switch on the microscope at the power switch 34 1 36 1 All motorized microscope components first undergo an initialization phase After initialization is complete the display on the stand shows the current microscope setting Fig 37 The microscopic components such as dia phragms condenser light and phase rings are already pre centered in the factory However re centering may be necessary due to transpor tation and assembly Before proceeding with the necessary steps first familiarize yourself with the stand s display and control panel Caution 34 After turning on the gas discharge lamps the burner must be immediately adjusted There fore do
32. ing buttons for all motorized microscope functions additional variable function keys focusing knobs LC display DM5000 B with LeicaScreen touchscreen Computer Interface e RS232C Software Tools e Leica DMControl for Windows 2000 XP NT e with plugins for e customitsation e DM Operation remote control e basic Image Viewer CTR5000 For Leica DM5000 B only Electronics Box Separate control unit with power supply for 100W halogen lamp see p 8 electrical safety 11 3 Overview of the Instrument 13 12 111098 Fig 1 Leica DM4000 M left side of the stand with AET22 advanced ergotube 1 Eyepiece 8 Function keys field diaphragm 2 Eyepiece tube 9 Transmitted light incident light switch 3 Tube 10 Function keys aperture diaphragm 4 Objective nosepiece with objectives 11 Function keys Light intensity 5 Specimen stage with specimen holder 12 Focus dial with coarse and fine adjustment 6 Condenser 13 Variable function keys factory pre assigned 7 LC display 14 Lamp adjustment window 12 3 Overview of the Instrument 21 20 1918 Fig 2 Leica DM4000B right side of the stand with Advanced Ergotube AET22 15 Lamp housing for incident light 16 Lamp housing for transmitted light 17 Transmitted light filter optional 18 Transmitted light filter optional 19 Variable function keys factory pre assigned 20 X Y coaxial drive height adjustable 21 Focus fine adjustment 22 Motorized filter cube exchanger 17 1
33. ing in the objectives see Objective Assembly p 19 When you rotate the objective into position the microscope automatically recognizes e the selected contrast method e the optimal settings for field and aperture diaphragm e the optimal condenser setting The objective magnification and the total magni fication appear in the display gt p 35 50 e Start with a small level of magnification Then switch to the next higher objective e For immersion objectives use the appropriate immersion medium OIL only use optical immersion oil according to DIN ISO standards Cleaning p 65 W Water immersion IMM Universal objective for water glycerol oil immersion Follow safety instructions for immersion oil Caution For lockable immersion objectives e Lock these by pushing the front part upwards until it stops approx 2 mm e Then after a gentle turning motion to the right the objective is locked Fig 64 For objectives with corrective mounts e Turn the knurl to adjust the objective to the thickness of the cover glass Fig 63 Immersion objective released MaI 7 Operation 7 7 Magnification Changer Optionally a coded magnification changer can be used which is manually operated On the knurled ring the following magnification factors can be set B Stand M Stand 1x 1x 1 25x 1 5x 1 6x 2X The selected factor is indicated in the display and included in the total magnifi
34. ion keys behind the focus dials TL BF appears in the first line of the display Fig 39 Stage with specimen holder 1 Object motion X direction 2 Object motion Y direction 3 Specimen holder 4 Condenser height adjuster 6 Startup Insert the specimen in the stage s specimen holder 39 3 Focus on the specimen The focus wheel on the left side of the stage allows focus adjust ment in large and small increments On the right side of the stage there is also a focus wheel for fine focus adjustment Set the light intensity using the INT keys 38 3 Close the field diaphragm with the FD function key 38 2 until the edge of the diaphragm ap pears in the specimen plane Using the condenser height adjuster 39 4 ad just the condenser until the edge of the field diaphragm appears in sharp relief If the image does not appear in the middle of the field of view 41c the condenser must be moved into the middle of the field of view with the help of the two leveling screws 40 1 37 6 Startup e Open the field diaphragm just enough for itto Do not adjust the aperture diaphragm The aper disappear from the field of view 41d ture diaphragm is already set optimally for each objective A Caution 6 6 Checking Phase Contrast Rings Fig 40 Condenser centering Fig 41 K hler Illumination 1 Centering bolts a Field diaphragm not focused not centered b Field diaphragm focused but not centered c Fie
35. ion degree 2 Caution see enclosed manual Caution Caution The power plug may only be plugged into an outlet equipped with a grounding contact The microscope s electrical accessory com ponents are not protected against water Water can cause electric shock Protect the microscope from excessive tem perature fluctuations Such fluctuations can lead to the accumulation of condensation which can damage the electrical and optical components Ambient temperature 15 35 C Do not interfere with the grounding function by using an extension cord without a ground wire Any interruption of the ground wire in side or outside of the device or release of the ground wire connection can cause the device to become hazardous Intentional ground interruption is not permitted l Caution Caution Before exchanging the fuses or lamps be absolutely certain to switch off the main ower switch and remove the power cable Through connection to the grounding con p p nection ancillary equipment with its own and or extra power supply may be brought to the same ground wire potential For connections without a ground connector Leica Service must be consulted 3 Overview of the Instrument 3 Overview of the Instrument ci sana Imaging Procedure Transmitted Light Axis Incident Light Axis e transmitted light BF DF PH Pol DM5000 B also ICT incident light fluorescence e transmitted BF DF PH
36. ld diaphragm focused and centered Diameter is too small however d Field diameter light Field diameter view K hler Illumination If your microscope is equipped for the use of phase contrast the light rings that fit the objec tives are built into the condenser The light rings are already leveled in the factory However the leveling should be rechecked A Every objective is assigned its own light ring in the condenser disc Therefore a check must be performed for each objective When swiveling in a suitable objective for phase contrast the cor responding light ring is set automatically e Press the BF Bright Field button one of the variable function keys behind the focus dials Note Fig 42 Focusing telescope 1 Adjustable eyelens 2 Clamping ring for fixing the focus position eg 6 Startup e In the place of an eyepiece insert the focus ing telescope Fig 42 into the observation tube e Swivel in the phase contrast objective with the least magnification e Focus the ring structure 43a by slightly loos ening the clamping ring 42 2 and moving the evelens 42 1 e Retighten the clamping ring e Press the PH Phase Contrast button The ring diaphragm in the condenser is pivoted in If the light ring and the phase ring are not shown as arranged in Fig 43c the light ring must be leveled Fig 43 Phase contrast centering procedure PH phase contrast ring LR light ring a Conde
37. luminate immediately upon being switched on Re 10 Trouble Shooting Cause Remedy Make sure that voltage is impressed Make sure that the microscope is connected to the power supply Check the cable connections gt Inform service technician to change the fuses Open the shutter p 35 Check the connection of the lamp houses to the microscope Transmitted axis A Incident Fluo axis V Make sure that the lamps are connected to the power supply gt Inform service technician to change the fuses of the ebq 100 Remove all unneeded filters from the light path Center the lamp p 41ff Replace the old lamp p 20ff Be sure that there is no loose connection at the power supply Replace the old lamp p 20ff gt The ebg 100 must be switched on repeatedly Hot Hg lamps should cool down before switching on again 61 10 Trouble Shooting Problem Bright Field The specimen can not be brought into focus Dark Field No definite DF contrast is possible The image is unevenly or not uniformly illumi nated Undesirable stray light Phase contrast No phase contrast is possible 62 Cause Remedy Use the correct immersion medium Lay the specimen with the cover glass to wards the top Make sure that the cover glass thickness is correct and that is conform to the indication on the objective Check the condenser cent
38. ly 22 1 Fig 21 Rear side of stand 1 Incident light lamp housing receptacle 2 Transmitted light lamp housing receptacle 3 12V 100 W connection for transmitted light symbol A 4 12V 100 W connection for incident light symbol Q 5 Assembly Fig 20 106 z lamp housing on the side open 1 Cover raised 2 Collector 3 12 V 100 W lamp or gas discharge lamp in mount 4 Reflector mirror 5 6 7 Adjusting screw for x y reflector 8 Fastening screw for lamp mount 9 Socket for contact plug Fig 22 Rear side of the ebq 100 supply unit 1 Lamp connection 5 Assembly 5 7 Equipping the Incident Light filter turret Fig 23 Filter cube Fig 24 Filter cube front side back side The receptacles on the turret are numbered According to your equipment the individual filter and or reflector cubes have already pre assigned positions A list is provided along with your shipment Identification Sheet Insert the filter and reflector cubes in the follow ing manner e Equip the incident light turret only when the microscope is switched off Fig 25 Removing the front panel 1 Filter receptacle e Remove the face plate from the upper part of 2 Retention pin the microscope Fig 25 Turn the turret in any 2 on Dane direction until the locking pin engages e Insert the filter or reflector cube into the mounting in front of you according to the identification sheet provided To do so place the filter or reflector cub
39. ming cleaning and maintenance work Protect electrical components from moisture Microscopes in warm and warm damp climatic zones require special care in order to prevent fungus contamination The microscope should be cleaned after each use and the microscope optics should be kept strictly clean 11 1 Dust Cover A To protect against dust cover the microscope and accessories with the dust cover after each use Note Caution Let lamps cool down before covering the stand with a dust cover The dust cover is not heat resistant In addition condensation water may occur 11 2 Cleaning 64 Caution Residual fiber and dust can create unwanted background fluorescence Cleaning Coated Parts Dust and loose dirt particles can be removed with a soft brush or lint free cotton cloth Clinging dirt can be cleaned with all commercially available water solutions benzine or alcohol For cleaning coated parts use a linen or leather cloth that is moistened with one of these sub stances Caution Acetone xylene or nitro containing thinner can harm the microscope and thus may not be used Test cleaning solutions of unknown composition first on a less visible area of the unit Be sure that coated or plastic surfaces do not become matted or etched Cleaning the Stage Remove light colored spots on the stage by rub bing with paraffin oil or acid free Vaseline Cleaning Glass Surfaces Remove dust on glass surf
40. nser in bright field BF position b Condenser in phase contrast PH position Light ring LR not centered c Light ring and phase ring centered PH 39 6 Startup Insert the centering key through the corre sponding openings 44 1 in the condenser holder Turn the centering screws until the dark ring phase ring in the objective is congruent with the slightly narrower bright ring light ring in condenser 43 c Repeat the process for all other phase con trast objectives Remove the centering keys after the centering procedure A Note During change of objectives the centering keys must not remain in the openings of the condenser 6 7 Adjusting the Light Sources Transmitted Light Axis TL with 107 2 Lamp housing 40 Fig 44 Light ring centering Clamping screw 1 The 107 2 lamp housing with 12 V 100 W halogen lamp has a defined presetting The lamp need notto be centered Incident light axis IL with 106 z lamp housing e When a supply unit is used it is turned on first Activate the incident light axis using the TL IL function key FLUO Leica DM4000 B DM5000 B or IL Leica DM4000 M appears in the display e Insert the reflector for lamp adjustment Fig 45 into the filter turret in place of a filter cube See p 26 Note the name of the exchanged filter cube Turn the reflector into the light path The reflector has reached the correct position when the name of the exc
41. nto the socket until it stops e Remove the dust cover e Reinsertthe lamp mount and retighten the fas tening screw 16 8 N Do not remove the lamp s dust cover until af ter you have installed the lamp Be certain to avoid getting fingerprints on the lamp Caution e Close the lamp housing and retighten the fas tening screws Fig 17 Lamp mount with 12 V 100 W halogen lamp e Place the lamp housing in the incident light lamp housing receptacle 18 1 and fasten it with the clamping screw on the side e Connect the lamp housing to the power supply for incident light symbol Q 18 4 Fig 18 Rear side of stand 1 Incident light lamp housing receptacle 2 Transmitted light lamp housing receptacle 3 12V 100 W connection for transmitted light symbol A 4 12V 100 W connection for incident light symbol 7 22 DD 5 Assembl Inserting the gas discharge lamps Hg and Xe into the 106z lamp housing Hg and Xe lamps are powered by the separate ebq 100 supply unit Read the separate instruction manual provided with this supply unit The following gas discharge lamps may be used and require different lamp mounts Fig 19 Type Typical bulb life 50 W high pressure mercury burner alternating current 100 hrs 100 W high pressure mercury burner direct current stabilized not stabilized 200 hrs 100 W high pressure mercury burner direct current stabilized not stabilized type 103 W 2 300 hrs 75 W
42. nual 1 Important Notes about this Manual Caution This operating manual is an essential com ponent of the microscope and must be read carefully before the microscope is put into operation or used Text symbols and their meanings 1 2 p 20 N o This operating manual contains important in structions and information for the operational safety and maintenance of the microscope and accessories Therefore it must be kept and taken care of Numbers in parentheses such as 1 2 corre spond to illustrations in the example Figure 1 Item 2 Numbers with pointer arrows for example p 20 point to a certain page of this manual Special safety instructions are indicated with the triangle symbol shown here and have a gray background Caution The microscope and accessories can be damaged when operated incorrectly Explanatory note Item not contained in all configurations 2 Safety Notes 2 Safety Notes 2 1 General Safety Notes 2 2 Electrical Safety This safety class 1 device is constructed and General specifications tested in accordance with EN 61010 1 IEC 1010 1 Leica CTR5000 electronics box for DM5000 B safety regulations for electrical measuring con trol and laboratory devices For indoor use only Supply voltage 90 250 V Frequency 50 60 Hz Caution Power input max 290 VA Fuses T6 3 A In order to maintain this condition and to en IEC 60127 2 3 sure s
43. o place Select the DIC contrast method Do so by pressing the DIC variable key Alternatively Press the CHANGE RL variable key For key occupation please see Identification Sheet The display indicates ICR The ICR filter cube containing polarizer and analyzer is automatically brought into the light path on the incident light axis Insert the objec tive prism slide into the prism slot 71 2 Alternatively 60 Rotate the ICR polarizer 71 3 and the IC P analyzer 72 1 on the stand manually into the light path Insert the objective prism slide into the prism slot 71 2 For fine adjustment rotate the knurled screw 71 1 on the objective prism slide 9 2 Transmitted Light 9 2 1 Bright Field Switch to the transmitted light axis by pushing the TL IL button Select the BF bright field contrast method Do so by pressing the BF variable key Alternatively Press the CHANGE RL variable key For key occupation please see Identification Sheet The display indicates BF Insert a transmitted light specimen Rotate an appropriate objective into place Use the focus dial to bring the image into fo cus and set the brightness using the INT func tion key 10 Trouble Shooting Problem Stand The microscope does not respond Illumination The image is completely dark The image is unevenly or not uniformly illumi nated The illumination flickers The lamp does not il
44. or setting the ap erture diaphragm AP are locked 8 1 3 Dark Field TL Switch to the transmitted light axis TL by pushing the TL IL button Select the DF dark field contrast method Do so by pressing the DF variable key Alternatively Press the CHANGE TL variable key For key occupation please see Identification Sheet The display indicates DF The dark field ring dark field stop is set au tomatically Insert a transmitted light specimen Rotate an appropriate objective into place Bring the image into focus using the focus dial and set the brightness using the INT function key A Notes The maximum objective aperture which may be used for dark field is 0 75 All objectives with greater aperture are automatically blocked for this procedure DF flashes in the display The microscope automatically selects the correct light ring in the condenser When selecting the dark field method the aperture diaphragm is opened completely and may not be adjusted To avoid errors in opera tion the function keys for setting the aperture diaphragm AP are locked 8 Imaging Procedure for Leica DM4000 B DM5000 B 8 1 4 Polarization TL Switch to the transmitted light axis TL by pushing the TL IL button Select the POL polarization contrast method Do so by pressing the POL variable key Alternatively Press the CHANGE TL 9 variable key For key occupation please see Identification Sheet
45. phragm FD are al ways set correctly The values for INT AP and FD can be changed individually This overwrites the previous setting Actual settings are stored automatically The specifications for INT AP and FD always relate to the currently activated light axis trans mitted light or incident light In addition to the preset function keys for INT AP and FD there are also variable function keys Variable function keys e These function keys are assigned logical functions before delivery see Identification Sheet e These functions can be reprogrammed according to your individual wishes A Note Reset Function The microscope can be reset to the default functions programmed atthe factory e When the microscope is switched off press all 3 variable function keys on the left stand section e Switch on the stand e Hold the keys pressed down until initialization is complete The standard information is shown in the display Switch off the instrument and switch it on again The settings are stored now 6 2 Switching on the Microscope e First swivel the objective with the least magnification into position 31 6 Startup Possible Assignments for the Function Keys For Leica DM4000 B DM5000 B Function key BF PH ICT DF POL CHANGE TL O INT T INT J AP 7 AP J FD T FD SHUTTER TL FLUO CUBE 1 CHANGE CUBE U CHANGE CUBE O SHUTTER FLUO INT FLUO T INT FLUO J FD FLUO T FD FLUO J COMB
46. right or left on an imaginary center line of the centering plane Fig 51 Then pivot the arc s mirror image with the ad justing knobs 46 2 and 4 and focus it using the reflector 46 3 Use the adjusting knobs 46 2 and 4 to orient the mirror image symmetrically to the direct image Fig 52 Defocus the image with the collector knob 46 6 until the arc image and mirror image are no longer recognizable and the image is uniformly illuminated Exchange the reflector cube for lamp adjustment for the original filter cube Centering the Hg 100 W and Xe 75 W mercury lamps In the adjustment window you see the direct arc image and the mirror image which in most cases are shifted together 6 Startup Fig 50 Direct arc image focused but decentered in reality the image is less focused Fig 51 Direct arc image in target position in reality the image is less focused Fig 52 Direct arc image and mirror image in target position in reality the image is less focused 43 6 Startup Focus the direct image with the collector 46 6 Use the adjusting buttons to pivot the arc s mirror image on the rear side of the lamp housing 46 2 46 4 to the side or completely out of the beam path The arc s focused im age remains visible Fig 53 Use the adjusting buttons 46 1 and 5 to place the direct arc image in the middle of the centering plane whereby the bright tip of the arc the focal spot should
47. rmed in a dust free room which is free of oil vapors and other chemical vapors as well as extreme humidity Atthe workplace large temperature fluctuations direct sunlight and vibrations should be avoided These conditions can distort measurements and micrographic images Allowable ambient conditions Temperature 15 35 G Relative humidity maximum 80 up to 30 C Microscopes in warm and warm damp climatic zones require special care in order to prevent the build up of fungus See the chapter Care of the microscope p 64 for additional instructions N Electrical components must be assembled at least 10 cm from the wall and away from flammable substances Caution i 4 Unpacking the Microscope Transport For shipping or transporting the microscope and its accessory components the original packaging should be used As a precaution to prevent damage from vibra tions the following components should be disas sembled and packaged separately e Unscrew the objectives e Remove the condenser e Remove the stage e Remove the lamp housings e Disassemble the burner of 106 z lamp housing e Remove all moving or loose parts 15 Si 5 Assembly 9 Assembling the Microscope The microscope components are logically as When using intermediate systems and optical sembled in this order accessories the sequence may vary In this case read Chapter e Stage 5 10 Optional accessories p 29 e Con
48. s far as it will go On the underside of the condenser there is an orien tation pin 6 1 which must be located in the guiding notch 7 1 Pull the condenser s clamping screw 5 3 so that the condenser is locked in place Connect the condenser over the connection 8 1 with the stand Condenser holder Condenser holder Condenser centering Clamping screw for condenser Condenser height adjuster Note The condenser must be centered before using the microscope K hler illumination p 37 Fig 6 Underside of condenser 1 Orientation pin Fig 7 Condenser holder 1 Guiding notch Fig 8 Condenser connector 1 Condenser cable socket 18 5 3 Tube and Eyepieces The tube is mounted to the stand either directly or with the use of intermediate modules It is fastened in place with the side clamping screw 9 1 e Loosen the clamping screw 9 1 e Insert the tube in the circular receptacle dovetail ring e Retighten the clamping screw 9 1 e Only for the MBDT motorized tube Connect the tube to the stand with the con nector socket 10 1 e The eyepieces are inserted into the eyepiece tubes on the tube 6 4 Objectives The receptacles on the objective turrets are numbered Fig 11 The individual objectives have already pre assigned positions at the factory according to their configuration A list of the exact objective positions is provided in shipment Identification Sheet
49. same time switches between coarse and fine setting The display indicator changes accordingly p 52 The AP 38 4 keys for the aperture diaphragm and FD 38 2 for the field diaphragm are used to set each diaphragm The optimal values are automatically preset when selecting the con trast method Fig 38 Defined Function Keys 1 Transmitted light incident light 2 Field diaphragm 3 Light Intensity 4 Aperture diaphragm 36 Variable function keys A factory preset is performed which fits your microscope configuration The function keys are labeled accordingly and a separate description of the key occupation accompanies the microscope Identification Sheet Abbreations are listed on p 32f 6 5 K hler Illumination For each objective optimal values for the aper ture diaphragm and the field diaphragm are al ready set The condenser is also already adjusted in the factory However depending on how the condenser is disassembled and reassembled it may be nec essary to re adjust the condenser in some cases Therefore check the condenser centering The following procedure is provided for the transmitted light bright field illumination moderate e Select an objective with magnification 10x 40x Activate the transmitted light axis by pushing the TL IL button 38 1 TL appears in the first line of the display Choose bright field as the contrast method by pressing the BF one of the variable funct
50. se Contrast Rings 39 Adjusting the Light Sources 40 Opera ee ee 46 Switching on the Microscope 46 Stages and Specimen Displacement 46 OC ES er 47 A A ATA Eyepieces eeeeeeensnnennenennnnensnnensnnennnnnennennn 49 Objectives roi 50 Magnification Changer 51 LIS ae 52 Aperture Diaphragm and Field Diaphragm sn 52 Imaging Procedure for Leica DM4000 B Leica DM5000 B 53 Transmitted Light aussen 53 8 1 1 Bright Field 53 8 1 2 Phase Contrast ans 53 8 1 3 Dark Field nes 54 8 1 4 Polarization anni 55 8 1 5 Differential Interference Contrast 56 Contents 8 2 FIUORBSCENLE nie 57 9 Imaging Procedure for Leica DM4000 M 58 9 1 Incident Light nen 58 9 1 1 Bright Field nenn 58 9 12 Dark Field ne 58 9 1 3 Polarizati n nee 59 9 1 4 Interference Contrast 60 9 2 Transmitted Light nenne 60 9 2 1 Bright Field u a 60 10 Trouble Shooting 61 11 Care of the Microscope 64 TL1 Dust LOVE nennen 64 11 2 Lili 64 11 3 Handling Acids and Bases 65 12 13 14 15 Essential Wear and Spare Parts 66 Abbreviations and Pictograms 67 LI 5 ARR REA 68 EU Declaration of Conformity 69 1 Important Notes about this Ma
51. stions regarding potential mistakes within this documentation The information in this manual is subject to modifi cation at any time and without notification Contents 1 2 1 2 2 5 1 5 2 5 3 5 4 9 9 5 6 9 7 5 8 5 9 5 10 5 11 5 12 Important Notes about this Manual 7 Safety Notes rn 8 General Safety Notes 8 Electrical Safety ses 8 Overview of the Instrument 10 Unpacking the Microscope 14 Assembling the Microscope 16 Rls 17 CONDCNSe pri 18 Tube and Eyepieces 19 OC CUI nee 19 Light Sources for the Transmitted Light Axis cece 20 Light Sources for the Incident Light AXIS 21 Equipping the Incident Light filter turret 26 Polarizer and Analyzer 27 DIG Psa 28 Optional Accessories 29 Connection to the Power Supply 30 Connection to the CTR5000 Electronics Box 30 6 1 6 2 6 3 6 4 6 5 6 6 6 7 1 1 1 2 1 3 1 4 48 1 8 16 11 18 19 8 1 Contents SANI rici 31 Functional Principle sun 31 Switching on the Microscope 34 The Display Leica DM4000 B DM4000 M 35 The Function Keys n 36 K hler Illumination u 0 37 Checking Pha
52. the filter slide Insert the Booster Lens or Excitation Manager Push the cover back Insert the clamping ring Insert the filter slide into the front receptacle on the right side of the stand 32 1 33 1 Using two filter sliders the Excitation Manager can be inserted into the back receptacle 5 11 Connection to the Power Supply Fig 33 Insert of Excitation Manager 29 5 Assembly After completing the assembly work connect Only for the Leica DM5000 B the stand to the power supply using the power cable Fig 34 2 e Connect the microscope 36 1 to the 5 12 Connection to the CTR5000 Electronics Box Microscope jack 35 1 on the rear of the electronics box Use the cable with the 25 pin plug Fig 34 Rear side of stand Leica DM4000 B M 1 Power switch 2 Power supply e Connectthe electronics box to the power sup ply using the power cable 35 2 Fig 35 Rear side of electronics box CTR5000 Fig 36 Rear side of stand Leica DM5000 B 1 Microscope connection 1 Connection to the CTR5000 electronics box 2 Power supply 30 6 Startup 6 1 Functional Principle The microscope s most important functions may be easily accessed using function keys The microscope may be switched between various contrast processes by pressing a single button e The microscope recognizes the objective chosen and the respective contrast process There fore the values for intensity INT aperture diaphragm AP and field dia
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