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Hanna Instruments Thermometer HI 4221 User's Manual

1. Note If the reading is out of range will be displayed on the LCD 44 Relative mV MEASUREMENTS TER Channel 1 To measure the Relative mV of a sample Press and then ramv 10 enter Relative mV Measure mode e The instrument will display the measured Relative mV value 424 um on the LCD together with a short GLP information about the e W mV last calibration or Not Calibrated message if no Rel mV calibration was performed no Rel mV offset set Last Calibr Jan 18 2005 12 34 Offset 0 1 Notes If the measured mV potential is out of range will be displayed on the LCD f pressing while in Relative mV Measure mode the Relative mV offset screen will be dis E 424 7 Abs mv Relative mV played on the LCD Press to clear the existing Mammam Relative mV OF USE x A or i V u 10 adjust the Set the value for the relative mi offset Relative mV value and then press 10 save the Stable Absolute mV 474 7 mi return to Relative mV Measure mode Before performing a Rel mV calibration make sure that the electrode is immersed into the sample to be measured Use Up and Dlewn to adjust the Rel mV Press Accept to update current Rel mV em oo S Relative mV 45 ISE CALIBRATION amp MEASUREMENTS HI 4222 only ISE CALIBRATION For greatest accuracy it is recommended to calibrate the instrument frequently The instrument shoul
2. entry Far edit Language This option allows the user to choose the desired language in which all information will be displayed To select the Language 105253 System Setup Press while in Measure mode bn Saving Confirmation e Press Sem GLP Data PE UM Date amp Time e Use or v foselec the Language option eee eee Serial Communication e Press and use A or v highlight the Meter information desired language Portuguese e Press seee confirm your selection and return to the System Setup menu or press 0 return fo the System Setup l Po saeco wasan me oinen menu without changing Note After selecting the desired language a warning pop up is escape seer V displayed on the LCD informing the user that the selected language becomes the current language only after restarting the instrument Serial Communication This option allows the user to set the desired speed for the serial communication baud rate between the instrument and PC The meter and the PC program must have the same baud rate To set the Serial Communication 105126 System Setup e Press serup while in Measure mode pu Saving Confirmation 1 Serial Communication S600 bps Meter Information m Hestore Factory Settings A Press Select to view and choose the Setup menu or press to retum fo fe S
3. AgCl Electrolyte in FDA approved bottle 4x30 mL 68 ORP PRETREATMENT SOLUTIONS 70201 Test Solution 200 275 mV 500 mL bottle 7021 Test Solution 240 mV 500 mL bottle 70721 Test Solution 470 mV 500 mL bottle 70911 Reducing Pretreatment Solution 500 mL 70971 Oxidizing Pretreatment Solution 500 mL pH ELECTRODES All electrodes part numbers ending in B are supplied with a BNC connector and 1 m 3 3 cable as shown below 1043 Glass body double junction refillable combination pH electrode Use strong acid alkali T DIA 12 m mm 0 5 Et im HI 1053B Glass body triple ceramic conic shape refillable combination pH electrode Use emulsions HI 1083B Glass body micro Viscolene non refillable combination pH electrode Use biotechnology micro titration 12 mm 0 5 5 mm 3 mm 0 2 0 12 3 0 er mios O O O i 4 120 mm gt 47 1131B Glass body single junction refillable combination pH electrode Use general purpose HI 1330B 9 5mm DIA 12 mm 0 37 0 5 L Y P 120 mm 4 7 Glass body semimicro single junction refillable combination pH electrode Use laboratory vials HI 1331B 5mm DIA 5mm 0 2 4 m 120 mm 4 7 Glass body semimicro single junction refillab
4. V keys help the user select the desired character It is also possible to delete the last character by positioning the cursor on the Backspace character 7 7 and pressing 5 setect the modified options are saved automatically Date amp Time This option allows the user to set the current date amp time and the format in which they appear These parameters will be displayed on the Measure screens and also when storing measured data Set Date and Time This option allows you to set the current date year month day and time hour minute second Notes Only years starting with 2000 are accepted be selected with Set Time Format This option allows you to choose between 12 Hour AM PM time format and 24 Hour time format from the displayed pop up menu Set Date Format This option allows you to choose the desired date format from 6 available options DD MM YYYY MM DD YYYY YYYY MM DD Mon DD YYYY DD MM YYYY and YYYY Mon DD To set the Date amp Time e Press while in Measure mode e Press system Note Pres f sec and use A or v highlight the deed Lu you want to modiy A or V tosetthe desired en to save the modified value for Set Date and Time option For the other two options press confirm your selection and select one of the pions 0 V is press ve to accept the modified option s a Date amp Time Enter the date and time
5. highlight the desired option Restore Factory Settings Press Select to choose the events announced by beeper escape Se A V The following is a detailed description of the System Setup option screen Beeper This option allows the user to enable or disable the beeper When the beeper is enabled a specific beep will be heard when the reading becomes stable when an alarm condition is reached when pressing a key or if a wrong key is pressed Stability Indicator When the reading becomes stable the instrument delivers a medium beep only if this option is ON along with the Stable indicator on the LCD Alarm If this option is ON a continuous double beep will be heard each time the set limits in Measure mode are exceeded along with the Alarm indicator on the LCD Key Pressed If this option is ON a short beep will be heard each time a valid key is pressed Wrong Key If this option is ON a long beep will be heard when an incorrect key is pressed To set the Beeper Press while in Measure mode au o Usj A Jor v Beeper e Press se anduse A of v to highlight gt Indicator Alarm desired beeper status option you want to modify Key Pressed Wrong Feu changing Press Select ra choose the events announced bu beeper Escape Select Saving Confirmation When enabling this option a prompt will appear on the LCD alerting the user to save the modified values by
6. year month minate second Press lt Escape gt to exit in previous screen Press Edit to edit focused entry Press Next gt or lt Previous to select entry return to the editing mode Otherwise the modified option is pes If the time is changed with more than one hour before last pH ION calibration a pop up warning will appear on the LCD notifying the user that a date time conflict has occured and some time dependent modes could work improperly e g Measure GLP Log LCD Setup This option allows the user to set the Contrast the Backlight of the LCD and the Backlight Saver The Contrast parameter can be adjusted within 7 steps while the Backlight parameter within 4 steps The Backlight Saver can be set from 1 to 60 minutes or it can be OFF disabled All the changes are visible on the LCD for each parameter Note To set the LCD Setup Press serup while in Measure mode Press Use A or select the LCD Setup option Pes Saet QNdUSE nee keytohighlight the desired parameter Us A a TH y to adjust the contrast backlight or to set the desired saver time Bs mm menu If the instrument backlight is turned off after the set period of time press any key to turn it back on Adjust the contrast and backlight and press Escape Backlight Backlight Saver 1 minute Use Up and lt Down arrows ta modify the LCD settings Press Ment to mowe to the
7. E dit B ff E e Use v to select the Calibration option ID Set Reminder Period Press see anduse A of v Ist Clear Calibration desired option meom Press Select to set the Function of the Press sees 10 confirm your selection or press escape fo cancel operation A Edit Custom Butters If you want to use other buffers than the ones already memorized the Edit Custom Buffers option is available allowing you to set the desired pH buffers Up to five pH custom buffers can be set To edit set the Custom Butters Custom Buffers Press while in pH Measure mode dun Press Press s anduse A or v to highlight the Edit Custom Buffers option to if desired and confirm the setting by pressing uu M Press Buffer to choose the neut otherwise press f 59 to edit the selected custom buffer Buffer 1 Her e While in edit custom buffer menu press set the custom the desired custom buffer value 22 Press exe 10 exit custom buffer edit menu If the Saving Confirmation is enabled press ves accept the modified option 10 escape without saving or cv 10 return to the editing mode Otherwise the modified option is saved automatically e Use key to select the next custom buffer 10 be set or press escape 10 return to Calibration options
8. Jan 18 2005 ISE Setup Channel 1 pa Analyte Addition Stability Criteria aalute Subtraction Log Escape Select Press Select ta choose the reading mode far ISE concentration measurements e The Temperature Compensation option is also included in the Temperature parameter Temperature Compensation This option allows the user to enable disable the temperature compensation for the measurement of lon concentration To set the Temperature Compensation Press while in SE Measure mode Us v to select the Temperature option Temperature Compensation option Press anduse A or v oenoable disable the Temperature Compensation 34 11 05 09 Ta Jan 18 2005 ISE Setup Channel 1 Temperature Source Channel 1 Temperature Unit UE Manual Temperature 25 0 Temperature Compensation Disabled Escape Select Press Select ta set the temperature compensation option Note T an n ISE calibration was pertormed and ihe option is changed a warning message appears on the LCD informing the user to perform a new calibration or to set the previous option in order to perform accurate measurements Calibration This option allows the user to set all the data regarding the lon calibration process Standard Entry Type Two entry modes for the standard solutions used for calibration are available Manual Selection when the calibration is started the desired standard solution can be ma
9. Last Calibr Jan 18 2005 15 02 Electrode 1 00 e 13 13 53 Jan 18 2005 Channel 1 BuitoHold 11471 Measure Last Calibr Jan 18 2005 15 13 Electrode Cond 1 00 Start Continuous return to normal Measure mode pres Continuous i Reading Note If the reading is out of range will he displayed on the LCD Outside Cal Range feature warns the user if the current reading is EGER out of the calibrated area The calibrated area is that part of the pH range in which the calibration point assures an accurate reading If the reading is taken out of the calibration area the Outside Cal Range message will start blinking on the LCD The calibrated area is calculated in according with the pH resolution used during the reading To avoid having this message the calibration points have to be well distributed in the desired measurement range If measurements are taken successively in different samples it is recommended to rinse the electrode thoroughly with deionized Etecuode water or tap water and then with some of the next sample to 2 Star prevent cross contamination and to condition the electrode before Debs f Chana immersing it into the sample solution The pH reading is affected by temperature In order to measure the pH accurately the temperature effect must be compensated To use the Automatic Temperature Compensation ATC feature
10. Use lt Up gt and amp Diewn arrows to set value Press lt gt to save current value Press lt Escape gt to exit in previous screen Escape EA V 11 00 34 Jan 18 2005 Channel 1 pH Setup Temperature Calibration Sample ID Stability Criteria pH Resolution Press Select ta choose the stability criteria during measurement Escape Select _is pressed and if the stability criterion is reached To set the Reading Mode pH Setup Press while in pH Measure mode Euri Temperature Calibration Press seip Sample ID Y Stability Criteria e Use v foselec the Reading Mode option TENER desired option pH Aesolution Press Select to choose the reading mode for measurements Escape Select Log This option allows the user to edit the logging settings Logging Type Logging Data Configuration Sampling Period and New Lot Logging lype Three logging types are available Automatic Manual and AutoHold With the Automatic type the values are logged automatically at constant time intervals while with the Manual type the user has the capability to log a measured value by pressing s With the AutoHold type the stored values are only the ones frozen on the LCD after the logging session was started To set the Logging Type pH Setup Press while in pH Measure mode CNW Logging Data Conti guratio m Press Setup Sampling Feriod e Us
11. gt c IO co 4 INO o co Mo Ll c co no NO co Tl c o MO c c 4 Qo 4 o l MO c 4 c MO i y c M2 o c NO Qo 4 o Tl co mmi A e MO oo M2 o xO D c oo co oo c BH MO NO 4 M2 o xO co oo o co INO co o o MO 441 T oo 4 INO M2 o xO oo Dm c T co oo o N o xO c MO MO oo onal o T oo 4 M2 o xO co Ll c 4 o co o 4 co MO BO co o MO gt N gt E CO co c D Ll oo gt BO co c 993 e 176 1767 3003 4 156 7 010 18 58 195 1700 sov 4172 s 709 sam 970 10908 _ 363 194 vo sop a17 6080 709 ast sz rosie 95 368 203 1807 2996 aam sama 70m 009 979 1073 DE rs aw BD During calibration the instrument will display the pH buffer value at 25 C 63
12. the sample volume previous calibration V the standard volume f the stoichiometric ratio between sample _ and standard and Ve V AMPLE m V sr 50 This feature allows the user to log pH mV or ISE HI 4222 only measurements together with temperature automatically The logging behaviour is dependent on the Logging Type and Reading Mode options from the appropriate unit setup The Logging Data Configuration options from the appropriate unit setup must be set first in order to be saved into the log report The maximum number of logged records is 5000 lot the maximum logging time is 24h and up to 100 lots can be saved Regarding data logging the available logging modes are shown in the table below Logging Mode LOGGING MODE 1 This logging mode can be used to monitor a chemical reaction e g biochemical processes By choosing this logging mode 54 will be available in Measure mode Note For 4222 the set S will be available in multichannel Measure mode depending on the focused channel To log data using this mode Measure Press Set while in Measure mode to start the logging session The Logging and the Sampling Period indicators will be displayed on the LCD and data will be stored at the set sampling period Note While automatic logging is running the measured unit setup is not available A warning message will be displayed on the LCD if the setup is accessed Chan
13. v toseled the Log option Penn Press anduse A or v highlight the Logging Type option Press se and use A or v to highlight the desired option cancel operation mE Escape Seer V logging Data Configuration This option allows the user to select the parameters that accompany a logged value Date Time Channel Calibration Data Sample ID Instrument ID Operator ID Company Name Aditional Info 1 and Aditional Info 2 27 To set the Logging Data Configuration ossing Data Config Press while in pH Measure mode ETE Sampling Period This option allows the user to select the desired sampling period for automatic logging type To set the Sampling Period UM Se Press serue while in pH Measure mode ers i Logging Type Press s oup x EC guration e Use v fo seled the Log option fion e Press see 10 confirm your selection or press ese 10 ee cancel operation New Lot Accessing this option a new manual lot can be generated next time you want to manually log a measured value Note If New Lot option is accessed and the Logging Type is Automatic a warning message appears on the LCD informing the user that a new lot can be created only if the Logging Type is set as Manual To generate a New Lot Press while in pH Measure mode Pressi s 28 e Use v toselect the Log option pH Setup Channel 1 Ei NE
14. 2005 12 53 ISE Fluoride TEMP 1 27 B c ere Known Addition Channel 1 148 9 First Reading Second Step Second Reading Stable 1 5 4 Sample Volume SA Buffer Val Standard Volume Standard conc Add 1 000 mL ISA buffer to 70 000 mL of Sample and immerse the electrode then press lt Continue gt Escape Continue 12 32 42 Jon 18 2005 Known Addition Channel 1 148 9 First Step First Reading Stable MaProbe 1 z5 4 G Sample Wol ISA Val Std Val Std Cone then press Continue Escape Eh Previous Note Press Escape dt any time to stop the measurement and When the reading is stable press 10 store the first mV reading The second step of the method will be displayed on the LCD in which the user is notified to add the Standard Volume of set concentration to the sample The method parameters are also displayed on the LCD Press to change the desired method parameters as indicated in the first step of the method Press pem to take the second mV reading S u ss ISE Result When the reading is stable press rea 10 store the second mV reading The ISE measurement results will be displayed on the LCD Press sae 10 log the current results into a ISE Method Calculated Slope Reading 1 Sample ID Report 0 or press ees to return to ISE Measure mode
15. Alarm See pH Setup section page 20 Note The Alarm Limits Low and High are set in the selected concentration unit of the measured ion or chemical compound and are displayed in a scientific format value and value exponent 3 Isopotential Point This option allows the user to edit the isopotential point of the electrode used for ion measurements The ion selective electrodes have different isopotential points If temperature compensation is desired for the ion measurements the isopotential point value is absolutely necessary The isopotential point is always edited in ppm mg L units To set the Isopotential Point Press while in SE Measure mode Press key to save the modified value Press Escape to return to the lon Setup options If the Saving 4 Jan 18 2005 Isopotential Point Channel 1 Edit the value far izopotential point 5 Limit Low Limit High 0 07 ppm 1 00 5 ppm Use Up and Down arrows to set value Escape A VO Note lf an ISE calibration was performed and the Isopotential Point option is changed a warning message appears on the LCD intorming the user to perform a new calibration or to set the previous option in order fo perform accurate measurements ISE Significant Digits Accessing this option the number of ISE significant digits can be set with one x two xx or three significant digits To set the ISE
16. key is not e Press Escape 210 return to the main menu Log History Accessing this option last logged records will be displayed on the LCD The log history list also contains the appropriate mV values the logged temperature the temperature compensation source as well as the records time stamp Note If data were logged the Log History Empty message will be displayed on the LCD To access Ine History displaying mode Press The instrument will display the log history regarding the selected Measure mode 10 11 25 Jan 18 2005 MIeasure Last Calibr Jan 18 2005 03 28 Electrode Cand 100 Channel 2 Last Calibr Jan 18 2005 10 40 Electrode Cond 1 003 le Notes When an alarm condition is active all logged records will have an exclamation mark If another Measure mode is selected the Log History will reset If the temperature unit is changed all logged temperature values will be automatically displayed in the new temperature unit 13 SYSTEM SETUP The System Setup menu allows the user to customize the user interface consult the meter information set the external serial communication interface and to restore the manufacturer settings Accessing System Setup System Setup Press while in Measure mode e Press Swem The system setup options will be displayed on the LCD To access a System Setup option Serial Communication Meter Information e Use or v
17. 931001 pH and ORP electrode simulator with LCD and 1 m 3 3 coaxial cable ending in female BNC connectors 7662 T Temperature probe with 1 m 3 2 cable HI 92000 Windows compatible software 920010 90 9 pin RS232 cable 4 RECOMMENDATIONS FOR USERS Before using these products make sure they are entirely suitable for the environment in which they are used Operation of these instruments in residential areas could cause unacceptable interferences to radio and TV equipment requiring the operator to follow all necessary steps to correct interferences The glass bulb at the end of the pH electrode is sensitive to electrostatic discharges Avoid touching this glass bulb at all times During operation ESD wrist straps should be worn to avoid possible damage to the electrode by electrostatic discharges Any variation introduced by the user to the supplied equipment may degrade the instruments EMC performance To avoid electrical shock do not use these instruments when voltages at the measurement surface exceed 24 Vac or 60 Vdc To avoid damage or burns do not pertorm any measurement in microwave ovens Hanna Instruments reserves the right to modify the design construction and appearance of its products without advance notice 9 SALES AND TECHNICAL SERVICE CONTACTS Australia Tel 03 9769 0666 Fax 03 9769 0699 China Tel 10 88570068 Fax 10 88570060 Egypt Tel amp Fax 02 2758 683 German
18. AmpHel electrodes If the electrode does not respond to pH changes the battery run down and the electrode should be replaced MEASURE Rinse the pH electrode tip with distilled water Immerse the tip bottom 4 cm 1 7 in the sample and stir gently for a few seconds For a faster response and to avoid cross contamination of the samples rinse the electrode tip with a few drops of the solution to be tested before taking measurements STORAGE PROCEDURE To minimize clogging and assure a quick response time the glass bulb and the junction of pH electrode should be kept moist and not allowed to dry out Replace the solution in the protective cap with a few drops of HI 70300 or HI 80300 Storage Solution or in its absence Filling Solution 7071 or 8071 for single junction and 7082 or 8082 for double junction electrodes Follow the Preparation Procedure on page 64 before taking measurements Note NEVER STORE THE ELECTRODE IN DISTILLED OR DEIONIZED WATER PERIODIC MAINTENANCE Inspect the electrode and the cable The cable used for connection to the instrument must be intact and there must be no points of broken insulation on the cable or cracks on the electrode stem or bulb Connectors must be perfectly dean and dry If any scratches or cracks are present replace the electrode Rinse off any salt deposits with water For refillable electrodes Refill the reference chamber with fresh electrolyte HI 7071 or HI 8071 fo
19. Buffer Edit Butter Group Accessing this option the user can edit the desired group of five pH buffers for automatic buffer recognition Automatic Buffer Entry Type If the Buffer Group already contains five pH buffers at least one pH buffer has to be removed in order to add another buffer To edit set the Buffer Group UE Press while in p Measure mode these Available Buffers e Pes et 1 673 3 000 4 010 5 000 7 010 3 177 10 010 21 e Use v fo select the Calibration option Press E and use Ej and Mj to choose the p buffer to be induded in the buffer group to from the buffer group Pese Tess gt _ Calibration Reminder In order to have accurate readings the instrument must be calibrated frecquently Three options are available for the calibration reminder Daily Periodic or Disabled To set the Calibration Reminder pH Setup Press while in pH Measure mode thine Manual Selection Point w Edit Custom Buffers cn gets Edit Buffer Group Calibration Reminder Disabled e Press see fo confirm your selection or press 10 reminder type or to disable cancel operation MM Escape Seer V 23 Set Reminder Period If choosing Daily or Periodic options for the Calibration Reminder the Set Remind Period must be accessed in order to set the time interval until next calibration The time interval between two calibrations can be set
20. Escape gt to exit in previous screen Escape Accept pH Resolution Accessing this option the desired pH resolution can be set with one two xx or three x xxx decimals To set the pH Resolution 11 03 31 E Jan 18 2005 pH Setup Press while in pH Measure mode Channel 1 Press sup i Use v to select the pH Resolution option desired option pH Resolution Press Select ta set the pH resolution far direct reading cancel operation Escape Select 31 The mV Setup menu allows the user to set the parameters associated with mV and Relative mV measurements These parameters can be set specifically for each channel HI 4222 only The settings will be applied only to the active channel Accessing mV Setup UP Press while in Measure mode and then mv Temperature 22 5 i Sample ID o select mV mV Rel range for the desired channel Emme i Press and then V to access mV Setup menu To access a mV Setup option The following is a detailed description of the mV Setup option screens source and units ewe A V Temperature See pH Setup section page 20 Sample ID See pH Setup section page 20 Stability Criteria See pH Setup section page 20 Reading Mode See pH Setup section page 20 Log See pH Setup section page 20 Alarm See pH Setup section page 20 Note The Alarm Limits Low and High are set in mV units 32
21. ISE SETUP HI 4222 only The ISE Setup menu allows the user to set the parameters regarding ISE measurement and calibration These parameters can be set specifically for each channel The settings will be applied only to the active channel Accessing ISE Setup ISE Setup Press while in Measure mode and then sc to 8 Press and then ss 10 access ISE Setup menu To access an ISE Setup option e Use v highlight the desired option lsopotential Point Press serce access the selected option ISE Significant Digits The following is a detailed description of the ISE Setup option Press Select ta choose the reading em owe AT Reading Mode This option allows the user to select the desired reading mode Direct Direct AutoHold Known Addition Known Subtraction Analyte Addition and Analyte Subtraction Except Direct and Direct AutoHold all the other methods are incremental see ISE Theory section for details page 54 Direct If using Direct reading mode the ion concentration can be read directly from the instrument Make sure the instrument was calibrated before taking measurements Direct AutoHold If using Direct AutoHold reading mode the ion concentration can be frozen on the LCD after starting the AutoHold mode Known Addition In the Known Addition method a standard with a known concentration of the measured ion is added to the sample The difference in mV potential is then used to calculate
22. J e Use v o select the Sample ID option Text Editor Press see to confirm your selection f the selected increment mode is None the Text Editor menu will be 22222 displayed on the LCD allowing you to enter the desired sample TEUER ME number name by accepting the highlighted character which is added escape se P V the user to select the desired character It is also possible to delete the last character by positioning the cursor on the Backspace character and pressing sees return to the editing mode Otherwise the moditied options are saved automatically cancel operation Stability Criteria This option allows the user to select the signal stability criterion for the measured parameter pH mV ISE Fast this criterion will give faster results with less accuracy Medium this criterion will give medium speed results with medium accuracy Accurate this criterion will give slower results with high accuracy To set the Stability Criteria Press while in pH Measure mode e Press ft i Use desired option e Press see 10 confirm your selection or press escape to cancel operation Reading Mode This option allows the user to select between Direct and Direct AutoHold pH reading mode If choosing the second Auto Hold 11 00 18 Jan 18 2005 Channel 1 Edit Sample ID Edit a numeric value for sample identifier 001 Limit Low 001 Limit High 333
23. Logging Type New Lot option Logaing Data Configuration Press confirm or press 10 escape without saving and return to the Log options The next manual record wil be stored in a new Lar Are you sure you want t continue Press generate a new manual lag Alarm This option allows the user to edit the alarm settings Alarm State and Alarm Limits If the Alarm option is enabled a continuous double beep will be heard along with the Alarm indicator blinking on the LCD each time the set limits in Measure mode are exceeded Alarm State Three modes are available for the Alarm State option Disabled the alarm will be disabled Inside Limts the alarm will notify the user when the measured value is inside the set limits Outside Limits the alarm will notify the user when the measured value is outside the set limits To set the Alarm State pH Setup Press setup while in pH Measure mode wa p Disabled Alarm State sus Alarm Limita e Use v to select the Alarm option essel i qe 1 Alarm Limits This option allows the user to set the alarm limits for the measured value Note The Alarm High value can not be lower than the Alarm Low value 29 To set the Marm Limits Press while in pH Measure mode Alarm Limits option Bos entry to be edit value then press Accept 10 save the modifie
24. Significant Digits Press while in SE Measure mode desired option Press select to confirm your selection or press 5 escape 10 cancel operation 38 11 13 31 Jan 18 2005 ISE Setup Channel 1 Reading Mode Temperature Concentration Unit Sample ID Stability Criteria Log Alarm lsopotential Point ISE Significant Digits Press lt Select gt to set the number of significant digits For concentration Espe Seea V pH CALIBRATION amp MEASUREMENTS pH CALIBRATION Calibrate the instrument often especially if high accuracy is required The instrument should be recalibrated e Whenever the pH electrode is replaced e At least once a week e After testing aggressive chemicals When No pH Calibration or pH Calibration Expired message appears on the LCD in the Reminder messages area PREPARATION Pour small quantities ot the butter solutions into clean beakers If possible use plastic beakers to minimize any EMC interferences For accurate calibration and 10 minimize cross contamination use two beakers for each buffer solution One for rinsing the electrode and one for calibration If you are measuring in the acidic range use pH 7 01 or 6 86 as first buffer and pH 4 01 3 00 or 1 68 as second buffer If you are measuring in the alkaline range use pH 7 01 or 6 86 as first buffer and pH 10 01 9 18 or 12 45 as second buffer For extended range measurements acidic and al
25. Y the activity coefficient C the concentration of the ion being measured In very dilute solutions Y approaches 1 so activity and concentration are the same Actual samples that are more concentrated have much smaller activity coefficients Y lt 1 The addition of an inert background salt to standards and samples stabilizes the activity coefficient so that concentration measurements may be made directly Hanna s lonic Strength Adjustment Buffer ISAB formulations also may optimize pH and complex interferences in addition to standardizing the ionic strength 54 The Nernst equation can be rewritten E E Slog C ION SELECTIVE ANALYSIS METHODS Direct Analysis This method is a simple procedure for measuring multiple samples H should only be used in the linear working regions of the sensor A direct reading instrument such as the HI 4222 determines concentration of the unknown by a direct reading after calibrating the instrument with the standards The instrument is calibrated as described in ISE CALIBRATION amp MEASUREMENTS section with two or more freshly made standards that are in the measurement range of the unknowns lonic strength adjustment is made to samples and standards Unknowns are measured directly by the instrument At lower concentrations in non linear regions of the electrode response multiple calibration points will extend measurements to a practical detection limit Calibrations must be performed more
26. and the value of the selected calibration buffer is significant If this message is displayed check if you have selected the appropriate calibration buffer Wrong buffer temperature this message appears if the buffer temperature is out of the defined buffer temperature range Clean the electrode or check the buffer Press lt Accept gt to update calibration this message alerts the user that some dirt or deposits could be on the electrode Refer to the electrode Cleaning Procedure see page 65 Slope too low Please check the buffer Slope too high Please check the buffer these messages appear if the current slope is under 80 or over 110 of default slope Recalibrate the instrument using fresh buffers Slope too low Press Clear Cal gt to dear old calibration Slope too high Press lt Clear Cal to dear old calibration these messages appear as a result of an erroneous slope condition Follow displayed instructions 4 Unrecognized buffer Please check the buffer or the buffer list for Semiautomatic and Automatic buffer entry type this message appears if the current buffer value is not close to any of the buffers from the buffer list group Check if the current buffer is present in the buffer list or the appropriate buffer group was selected The current buffer was already calibrated Press lt Accept gt to recalibrate in this point or change Follow displayed instructions pH MEASUREMENTS Make sure the instrument has
27. buffer and stir gently The temperature probe should be close to the pH electrode Select the used pH calibration buffer with 0 or Pievos The Please wait message will appear on the LCD unti the reading is stable or the buffer is validated If the pH butter is validated will appear on the LCD Press acer update calibration The calibration buffer will be added to the Calibrated Buffers section Immerse the pH electrode and the temperature probe into the next buffer solution and follow the above procedure or press exe fo exit calibration Notes The new added calibration point will replace an old one if the difference between them is 0 2 pH pH Calibration Channel 1 e f the existing stored calibration is full five calibration points a pop up menu will be displayed on the 0 in which you select with lt gt keys the buffer you want to replace with the current buffer Press Remove 10 delete the selected buffer and then press acce to update calibration with Use Left and lt Right arrpws to select the new buffer which old buffer want ta be removed When in MTC mode if pressing see after entering pH calibration and while a HANNA pH calibration buffer with a resolution is selected a pop up menu will SE E be displayed on the LCD in which the temperature value be ajusted using wv Press Accent fo sav
28. calibration The calibration point value will be added li Calibrated section e The Please wait message will appear on the LCD until the reading is stable and 10 seconds elapsed time in which immerse the lon Selective Electrode and the temperature probe into the next standard solution and follow the above procedure or press eee 10 exit calibration 13 02 18 Jan 18 2005 Notes The new added calibration point will replace an old one if the difference between them is less than 20 ETE of the standard solution ISE Calibration Stable If the existing stored calibration is full five calibra tion points a pop up menu will be kr ip on the LCD in which you can select with or bp the standard solution you want to ia with ihe current one Ri Remove to et the bration with the new standard If the isopotential point of the electrode is unknown the ion calibration and measurements can be performed only without temperature compensation see ISE Setup Tem perature option for details page 33 When the temperature compensation option is disabled at least a two point calibration must be performed in order to perform ISE measurements Otherwise will appear on the LCD When in MIC mode if pressing after enter ing ISE calibration and while a standard solution is selected a pop up menu will be displayed on the LCD in which the concentration and the erin value aie or hi Press Acce
29. connect and submerge the HI 7662 T temperature probe into the sample as dose as possible to the electrode and wait for a few seconds If the temperature of the sample is known Manual Temperature Compensation MIC can be performed by disconnecting the temperature probe Notes For single channel pH measurements HI 4221 or ATC indicators will be displayed on the LCD while for dual channel pH measurements HI 4222 or ATCI ATC2 indicators will be displayed depending on the temperature compensation mode and selected channel For mV Rel mV measurements NO PROBE or TEMP indicators will be displayed on the LCD HI 4221 only respectively NoProbe or TEMPT1 TEMP2 indicators 4222 only for mV Rel mV ISE measurements depending on the temperature compensation mode and selected channel When in MTC mode the temperature can be modified by pressing Tor pH Measure mode and for ml Rel mV Measure mode HI 4221 mn Ife Mode option is Direct HI 4222 only 1 Th value can be ajusted with from 20 0 C to 120 0 C Press m in ATC mode for pH respectively TEMP for 5 will be on the LCD if the measured temperature is under or over temperature range 20 0 C to 120 0 C 43 mV amp Relative mV MEASUREMENTS mV ORP MEASUREMENTS Oxidation reduction potential ORP measurements provide the quantification of the oxidizing or redu
30. data To choose the Basic displaying mode Press cisely While in Measure mode The Choose Display Configuration message will be displayed in the Reminder messages area Press 8 54 38 10 28 36 Jan 18 2005 Measure Jan 18 2005 Measure Channel 1 Stable 40 b pH Last Calibr Jan 18 2005 10 27 Electrode Cond 1 0077 28 e p H Channel 2 Stable 2 ppm Last Calibr Jan 18 2005 09 40 Electrode Cond 1 00 Last Calibr Jan 18 2005 10 15 TEMP ISE Fluoride 24 7 P EL s Accessing this option detailed GLP data will be displayed on the LCD for pH Measure and ISE Measure modes only Last Calibration date and time Offset and Slope values Calibration Buffers Standards and general information regarding the buffers standards the calibration temperature temperature compensation mode date and time For pH Measure the Electrode Condition is also displayed on the LCD in percent Note If only a one point pH calibration is performed or the current calibration does not include at least two consecutive standard buffers of pH 4 01 7 01 6 86 and 10 01 9 18 buffers the Electrode Condition will be unknown To access the GLP displaying mode 11 15 30 33 10 40 52 Jan 18 2005 Measure Jan 18 2005 Measure Channel 1 Channel 1 Last Calibr Jan 18 2005 10 34 Average Slope 100 024 Calibrated ag ISE Fluoride Last Calibr Jan 18 2005 10 22 Cond Channel 2 Offset 3
31. displayed on the LCD Select the desired lot with ol CY vew 10 display logged n from ihe highlighted lot The Please wait message will be displayed on the LCD for one second The selected Logging Data Contiguration options will be displayed on the LCD together with GLP information last calibration date and calibrated buffers standards if a calibration has been performed on the se lected mode and the logged values measured value mV value temperature value temperature compensation mode and the logging time and press Note For automatic logging only it is possible to view the plotted graph Press San to display the graph Dy pressing Sht jt is possible to move the graph along the X or Y axis with the arrow keys If pressing serue while the graph is displayed the zoom menu for the X and Y axes will be accessed Press Zoom Or Zoom Time A 4X switch between the active zooming axes and then zoom in or out on the selected axis by pressing the appropriate Hg key 01 13 27 08 Jan 18 2005 J 5 n mn gcn pH ij Auto Log Recall 12 Press View to view highlighted lot Press Setup to change options Press lt gt to filter log lots L 1z PH Automatic Hanna Channel 1 Jan 15 2005 16 20 16 01 Additional Info 2 Last Calibration Jun 18 2005 16 02 Sree 6 1 33 2 25 5 Jan
32. instrument on from the power switch located on the rear panel of the instrument Please wait until the instrument finishes the initialization process instruments Loading Note lt is normal for the loading process to take a few seconds If the instrument doesn t display the next screen restart the meter using the power switch If the problem persists contact your dealer CHANNEL SELECTION HI 4222 only Press While in Measure mode to access channel TIE selection menu Four available options will be displayed 2028 Channel 1 Channel 2 or multi channel with the first or the second channel focused The Choose Channel Configuration message is displayed in the Reminder messages area Last Calibr Jan 18 2005 10 22 MED Electrode Cand 10605 Select the desired option by pressing the appropriate key i or CK The instrument will enter in the selected option Measure mode Last Calibr Jan 18 2005 10 15 ISE Fluoride Choose Channel Configuration Channel 1 anne Channel 1 Channel 2 alae TE Channel 2 a DISPLAYING MODES For each measurement mode pH mV Rel mV or lon the following display configurations are available Basic Good Laboratory Practice GLP Graph and Log History Basic Accessing this option the measured value and its units are displayed on the LCD along with the temperature value temperature compensation mode and GLP
33. logging mode and then es again To stop the logging session press gt ee 8e Notes For the automatic logging if the maximum logging time 24h has been reached a warning pop up will be displayed on the LCD in order to stop the current log and start another one in a new lot e f 100 lots have been saved or maximum 5000 records have been manually stored a warning pop up will be displayed on the LCD in order to delete one lot or to select a new lot for the manual logging to log other records LOG RECALL This feature allows the user to view all stored data If no data were DUST logged the No records were found message will be displayed on 7 the LCD in the Log Recall screen Otherwise the instrument will display all the memorized lots in accordance with the selected option Automatic Log Manual Log or ISE Method Report HI 4222 only To view the memorized data Press while in Measure mode Press 5 The Choose Log Report Type message will Last Calibr Jan 18 2005 08 40 Electrode Cond 100 ATE be displayed in the Reminder messages area 97 Choose Log Report Type E Automatic Manual Method scaps Log Log Report 60 Report m logged Tis for the ae Log Report type will be displayed on the LCD To filter the displayed lots press and then the desired unit jor we HI 4222 only Only the selected measurement unit joi will be
34. peu I Sample Volume Standard Volume If pressing eae L the method parameters can be changed ISA Volume Standard Conc and the sample concentration will be recalculated Press X 9 o sta rt nother measurement Press 2 522 to return in main n s Press lt log the current rezults retum ISE Measure mode KNOWN SUBTRACTION To measure the concentration of a sample using the Known Subtraction incremental method Press mone and then to select SE Measure mode for the selected channel Select the Known Subtraction method see ISE Setup for details page 33 Pres 2 start the measurement The first step of the method will be displayed on the LCD in which the user is notified to add the appropriate ISA Butter Volume if different from 0 mL to the sample and to immerse the electrode into the Sample Volume The method parameters are also displayed on the LCD Press c fO set me Press previous 0 dd next previous parameter Note For th Known Subtraction method the Udine T ratio factor between standard and amk must also be set in order to take accurate measurements Make sure that the set parameters values are the used ones and submerge the lon Selective Electrode tip 4 cm 1 ri into the iis to be tested When the vein is stable press fo store the first mV reading The second step of the method will be displayed on the LCD in which the user is notified to ad
35. pressing To modify the Saving Confirmation option System Setup Press while in Measure mode Enabled Saving Confirmation GLP Data Date amp Time s Piesi e Use v toselectthe Saving Confirmation option Moo Serial Communication e Press Select u and Use u u Or x V u 10 highlight the Meter Information Restore Factory Settings cancel operation Press Select ta enable or disable the saving confirmation option GLP Data This option allows the user fo set general information which will appear in the log reports The options are available for both channels HI 4222 only and can have a max of 10 characters Operator ID this option allows you to edit the name of the operator Instrument ID this option allows you to edit an identification name number for the instrument Company Name this option allows you to edit the company name Additional Info 1 amp Additional Info 2 for general purpose notations 15 To set the GLP Data GLP Data e Press setup while in Measure mode nstrument 10 H Company Hame Pes m NE de Text Editor Use A V _ to select the GLP Data option desired option Press see 10 edit the desired information The Text Editor menu will be displayed on the LCD Enter the desired information by accepting the highlighted character which is added to the text bar using sect The gt and EE information entry
36. probes are used If no temperature probe is detected Manual Temperature Compensation will be performed with the MIC indicator on the LCD Temperature Unit Accessing this option the desired temperature unit can be chosen Celsius Fahrenheit or Kelvin degrees and the meter will automatically make the conversion for the selected unit Manual Temperature If no temperature probe is connected the desired temperature can be set manually The default setting is 25 C If the measured temperature is different the value can be manually adjusted in order to obtain an accurate pH reading 20 To set one of the Temperature options p m pH Setup Press while in pH Measure mode Channeli Temperature Source Channel 1 e Press pH i Temperature Unit Manual Temperature Pres se anduse A or v highlight the desired Temperature option you wish to modify e Press see and use A or v highlight the desired option for Temperature Source amp Unit options or use or Vv adjust the temperature value between source far current channel the displayed limits for Manual Temperature option sa A V Press seee 10 d selection for Temperature Source amp Temperature iid Ta press ese 10 cancel operation Calibration This option allows the user to set all the data regarding the pH calibration process Butter Entry Type Three entry modes for the pH butters used for calibration are avai
37. shipping costs prepaid When shipping any instrument make sure it is properly packed for complete protection To validate your warranty fill out and return the enclosed warranty card within 14 days from the date of purchase TABLE OF CONTENTS 2 PRELIMINARY EXAMINATION 4 GENERAL DESCRIPTION on 4 FUNCTIONAL DESCRIPTION J aaa aaa 5 SPECIFICATIONS on 8 OPERATIONAL GUIDE 9 DISPLAYING MODES c 1 SYSTEM SETUP 14 DH SETUP iet ttt 20 mV SETUP Lee 32 ISE SETUP 4222 only 33 pH CALIBRATION amp MEASUREMENTS nnn 39 mV amp RELATIVE mV MEASUREMENTS 44 ISE CALIBRATION amp MEASUREMENTS HI 4222 46 LOGGING 57 PC INTERFACE 62 pH BUFFER TEMPERATURE DEPENDENCE 63 ELECTRODE CONDITIONING amp MAINTENANCE aaa 54 TROUBLESHOOTING GUIDE aaa 66 TEMPERATURE CORRELATION FOR pH SENSITIVE GLASS 67 68 PRELIMINARY EXAMINATION Remove the instrument from the packing material and examine it carefully to make sure that no damage has occurred during shipping If there is any damage notify your dealer or the nearest Hanna Service Center The meters are supplied complete with 1131B Glass body Combination pH Electrode 7662 T Temperature probe e pH 4 01 amp 7 01 Buffer solutions 20 mL each e 700661 Cleaning Solution 2 x 20 mL 7071S Electrolyte solution 76404N Electrode Holder 2Vdc Power Adapt
38. up to 1 day 1 year for Daily Periodic options Note If Set Reminder Period parameter is accessed and the Calibration Reminder is disabled a warning message appears on the LCD informing the user that the reminder period can be set only if the Calibration Reminder is set as Daily or Periodic To set the Reminder Period Periodic Reminder Channel 1 Press while in pH Measure mode Enter the time period that must be passed since the last calibration before the time reminder will appear sao Use v to seled the Calibration option days hous minutes mmm qme 01 Press A jo V pa Reminder Period option Use lt gt and zDlown arrows to set value e Press Select and use Previous 10 select next previ OUS B fo be adit Press lt Escape gt to exit in previous screen Press Edit to edit focused entry Press Next gt lt Previous to select entry Press ue A jo V Escape i Previous Press Escape 10 relum to ti Calibration options If the Saving Confirmation is enabled press ve 10 accept the modified Option 10 escape without saving cance 10 return 10 the editing mode Otherwise the moditied option is saved automatically lear Calibration Accessing this option the existent pH calibration relative to the selected channel can be cleared If the calibration is cleared another
39. 18 2005 15 02 54 16 1 5 52 16 15 35 16 1 5 34 16 1 5 55 L 1z PH Automatic Hanna Channel 1 Jan 15 2005 16 20 16 20 3 Press Setup to select Zoom mode 1615354 A 161535 amp Jan 18 2005 15 20 16 gt To delete lots Auto Log Recall Press while in Log Recall mode M2PH Jan 18 2005 Pes s jor 589 to access delete or delete dl mode de Otherwise press vew 10 return to Lag Recall view mode 1 After selecting one of the deleting modes use or clan 17 to select one lot and then press 5 cin 1 to delete the selected lot or all lots The Please wait Jan 17 message will be displayed on the LCD until the selected lot or E all lots are deleted Press Setup to change options Press lt gt to Filter lag lots mode Note Logged lots should also be deleted whenever Please Delete Old Log Files or Low Data Logging Space message appears on the LCD in the Reminder messages area Data transmission from the instrument to the PC can be done with the HI 92000 Windows compatible software optional 92000 also offers graphing and on line help feature Data can be exported to the most popular spreadsheet programs for further analysis HI 4221 and HI 4222 instruments have two available serial interfaces RS232 and USB The desired serial interface can be selected from the settings window of the HI 92000 software If choosing the RS23
40. 2 serial interface use the optional Hanna HI 920010 cable connector to connect your instrument to a PC Make sure that your instrument is switched off and then plug one connector to the instrument RS232 socket and the other one to the serial port of your PC Note Other cables than HI 920010 may use a different configuration In this case communication between instrument and PC may not be possible If choosing the USB serial interface use a standard USB cable to connect your instrument to the For both serial interfaces make sure that the instrument and the HI 92000 software have the same baud rate and the appropriate communication port 62 pH BUFFER TEMPERATURE DEPENDENCE Temperature has an effect on pH The calibration buffer solutions are affected by temperature changes to a lower degree than normal solutions During calibration the instrument will automatically calibrate to the pH value corresponding to the measured or set temperature pH BUFFERS NM T s per soo ne zm so uno o Fem s ww sm on rox use om sme m ser n o om e Dems unam n en om m m um um sus m Jo 1 c c co o l MO c c c oo M2 xO co INO a c NO co c NO LL NI INO N N Un ON c co co g Mo
41. 4 mY Average Slope 100 5 100 Haia A Jan 18 2005 10 22 276 C Jan 18 2005 10 21 177 8 mv Last Calibr Jan 18 2005 10 40 TRIB 2 005 C Janie 2005 10 21 Offset 0 4 mv Slope 100 1 i Hanna Calibrated Pani Elec Cord sss 11 fess ee ee Graph Accessing this option the online graph with currently logged values pH mV Rel mV or ISE vs Seconds will be displayed If there is no active log the previously logged data for the selected parameter will be plotted Notes I no data were logged the graph displaying mode will not be accessible f no automatic log is saved the offline graph will not be available To access the offline online graph 15 43 32 10 44 38 Jan 18 2005 Measure Jan 18 2005 Measure Channel 1 Stable Last Calibr Jan 18 2005 09 40 ATC Electrode Cond 1 00 25 a 2 C Last Calibr Jan 18 2005 10 40 Electrode Cond 1 00 25 Start scape bea When the online graph is displayed Use move the graph along X iie axis When the a graph is displayed e Use the arrow keys to move the graph along X Time and Y parameter axes Press serur fo access the zoom menu for X and Y axes Use zm or Zom zom Time i 7em 0 switch between the active zooming axes Press zoomin OT zoom our iL zoom the selected o axis Note While in zoom graph menu the move
42. 5 and 5 custom buffers Up to five point calibration ISE Calibration 5 fixed standard solutions available for each measurement unit and 5 custom solutions Input channels Temperature compensation Manual or Automatic from p p 20 0 to 120 0 C 4 0 to 248 0 F 253 to 393 160 x 231 x 94 mm 6 3 x 9 1 x 3 7 1 2 Kg 2 6 Ib 0 50 C 32 122 F 273 323 K max 95 RH non condensing Environment OPERATIONAL GUIDE POWER CONNECTION Plug the 12 Vdc adapter into the power supply socket Notes These instruments use non volatile memory to retain the pH lon calibrations and all other settings even when unplugged e Make sure a fuse protects the main line ELECTRODE AND PROBE CONNECTIONS For pH or ORP measurements connect a pH ORP electrode with internal reference to the BNC connector located on the rear panel of the instrument for the desired channel HI 4222 only For ISE measurements HI 4222 only connect to the desired channel an ISE electrode with internal reference to the BNC connector located on the rear panel of the instrument For electrodes with a separate reference connect the electrode s BNC to the BNC connector and the electrode s reference to the reference input socket For temperature measurements and automatic temperature compensation connect the temperature probe to the appropriate socket for the desired channel HI 4222 only INSTRUMENT START UP Turn the
43. ELECTRODE CONDITIONING amp MAINTENANCE Reference Reference Fill Hole N L Fill Hole Sensing Wire Reference Wire Reference Sensing Wire Wire Reference Reference Junction Junction Glass c Glass Bulb R Bulb A Plastic Body Glass Body pH Electrode pH Electrode Reference Reference Wire Wire Reference Reference Junction Junction Platinum or Platinum or Gold tip Gold tip Plastic Body Glass Body ORP Electrode ORP Electrode PREPARATION PROCEDURE Remove the protective cap of the pH electrode DO NOT BE ALARMED IF SALT DEPOSITS ARE PRESENT This is normal with electrodes They will disappear when rinsed with water During transport tiny bubbles of air may form inside the glass bulb affecting proper functioning of the electrode These bubbles can be removed by shaking down the electrode as you would do with a glass thermometer If the bulb and or junction is dry soak the electrode in 70300 or HI 80300 Storage Solution for at least one hour For refillable electrodes If the filling solution electrolyte is more than 2 cm 1 below the fill hole add 7082 or HI 8082 3 5M KCI Electrolyte Solution for double junction or 7071 or HI 8071 3 5M KCI AgCl Electrolyte Solution for single junction electrodes For faster response unscrew the fill hole screw during measurements 64 For
44. IA 12 mm 0 37 0 5 from fo A 120 4 7 FC911B Plastic body PVDF double junction refillable with built in amplifier combination pH electrode Use very high humidity m 3 3 E mem 1 1 D o 3 3 Y l 72 ORP ELECTRODES HI 3131B Glass body refillable combination platinum ORP electrode Use fitration HI 3230B Plastic body PEI gel filled combination platinum electrode Use general purpose HI 4430B Plastic body PEI gel filled combination gold ORP electrode Use general purpose Consult the Hanna General Catalog for more electrodes with screw type or BNC connectors PEI is registered Trademark of General Electric Co PVDF is registered Trademark of Pennwalt Corp EXTENSION CABLE FOR SCREW TYPE ELECTRODES SCREW TO BNC ADAPTER HI 7855 1 Extension cable 1 m 3 3 long HI 7855 3 Extension cable 3 m 9 9 long HI 7855 SERIES CABLE CONNECTORS CONNECTOR AND 3 0 mm 0 12 CABLE WITH BNC CONNECT TO CONNECT TO THE SCREW TYPE BNC SOCKET ELECTRODES OF THE METER OTHER ACCESSORIES 710005 8 Voltage adapter from 115 Vac 12 Vdc 800 mA USA plug 710006 8 Voltage adapter from 230 Vac 12 Vdc 800 mA European plug 76404N Electrode holder HI 8427 pH and ORP electrode simulator with 1 m 9 2 coaxial cable ending in female BNC connectors HI
45. Instruction Manual HI 4221 amp HI 4222 pH mV ISE Temperature Bench Meters HANNA instruments www hannainst com Dear Customer Thank you for choosing a Hanna Instruments product This manual will provide you with the necessary information tor correct use of the instrument Please read this instruction manual caretully before using the instrument If you need additional technical information do not hesitate to e mail us at tech hannainst com or see the back side of this manual for our worldwide sales and technical service contacts These instruments are in compliance with C directives WARRANTY HI 4221 and HI 4222 are warranted for two years against detects in workmanship and materials when used for their intended purpose and maintained according to instructions Electrodes and probes are guaranteed for six months This warranty is limited to repair or replacement free of charge Damage due to accidents misuse tampering or lack of prescribed maintenance is not covered If service is required contact the dealer from whom you purchased the instrument If under warranty report the model number date of purchase serial number and the nature of the failure If the repair is not covered by the warranty you will be notified of the charges incurred If the instrument is to be returned to Hanna Instruments first obtain a Returned Goods Authorization number from the Technical Service Department and then send it with
46. Solution in FDA approved bottle 500 mL pH 7 010 Buffer Solution 500 mL bottle pH 9 177 Buffer Solution 500 mL bottle pH 9 18 Buffer Solution in FDA approved bottle 500 mL pH 10 01 Buffer Solution in FDA approved bottle 500 ml pH 10 010 Buffer Solution 500 mL bottle pH 12 450 Buffer Solution 500 mL bottle ELECTRODE STORAGE SOLUTIONS 70300L Storage Solution 500 mL bottle 803001 Storage Solution in FDA approved bottle 500 mL ELECTRODE CLEANING SOLUTIONS HI 70000P 70611 70731 70741 70 71 80611 80 31 80 71 Electrode Rinse Sachets 20 mL 25 pcs General Purpose Solution 500 mL bottle Protein Cleaning Solution 500 mL bottle Inorganic Cleaning Solution 500 mL bottle Oil amp Fat Cleaning Solution 500 mL bottle General Purpose Solution in FDA approved bottle 500 mL Protein Cleaning Solution in FDA approved bottle 500 mL Oil amp Fat Cleaning Solution in FDA approved bottle 500 ml ELECTRODE REFILL ELECTROLYTE SOLUTIONS HI 7071 HI 7072 HI 7082 HI 8071 HI 8072 HI 8082 HI 8093 3 5M AgCl Electrolyte 4x30 mL for single junction electrodes IM KNO Electrolyte 4x30 mL 3 5M KCI Electrolyte 4x30 mL for double junction electrodes 3 5M KCI AgCl Electrolyte in FDA approved bottle 4x30 mL for single junction electrodes IM KNO Electrolyte in FDA approved bottle 4x30 mL 3 5M KCI Electrolyte in FDA approved bottle 4x30 mL for double junction electrodes
47. The method parameters are also displayed on the LCD Press a to change the desired method 05 indicated in the first step of the method will be displayed on the 0 Press se 10 log the current results into a ISE Method Report or press 2e to return to SE Measure mode e f pressing a the method parameters can be changed and the sample concentration will be recalculated Press start another measurement 53 ISE THEORY An lon Selective Electrode ISE is an electrochemical sensor that changes voltage with the activity or concentration of ions in solutions The change in voltage is a logarithmic relationship with concentration and is expressed by the Nernst equation E E Slog a where E the measured voltage standard voltage and other standard system voltages a the activity of the ion being measured S the Nernst slope factor and is derived from thermodynamic principles S 2 303RT nF R the universal gas constant 8 314 J K mol T the temperature in degrees Kelvin F the Faraday s constant 96 485 C mol n the ion charge The slope may be positive or negative depending upon the ion charge n SLOPE mV decade Monovalent cation 59 16 Divalent cation 2958 Monovalent anion 59 16 Divalent anion 29 58 Activity and concentration are related by an activity coefficient expressed as a yC where a the activity of the ion being measured
48. ajusted using Or Vo Press oe 0 50 6 the new concentration value I the standard solution concentration is validated will appear on the LCD Press 10 update calibration The calibration point value will be added 10 the Calibrated Standards section The Please wait message will appear on the LCD for 10 seconds and until the reading is stable time in which immerse the ion selective and the temperature probe into the next standard solution and B To calibrate the instrument using T Standard entry type Press J If the instrument was calibrated before and calibration was not cleared the old calibration can be cleared by pressing a t After 10 seconds ce wil no longer be available Note It is very important to dear calibration history when a new electrode is used because most errors and warning messages that appear during calibration depend on calibration history Add ISAB to all standard solutions Immerse the lon Selective Electrode and the temperature probe approximately 4 m 1 4 into the less concentrated standard solution and stir gently When the stability condition is reached and 10 seconds elapsed a pop up menu will appear on the LCD in which the standard concentration value can be set e 4cm 11 27 Press owes Previous o select entry to be B Press a 000 8 A Or store ii set concentration VW 4 f the standard solution concentration is validated
49. an 18 2005 12 59 option New Lot generation ISE Fluoride LOGGING MODE 4 and be available in Measure mode Note For 4222 or v and m go will be available in multichannel Measure mode depending on the focused channel To log data using this mode was pressed When the measured value is frozen on the LCD by pressing 4 the stability criterion is reached the logged value is the one that has been frozen on the LCD 59 store another frozen value press cewe to return to normal logging mode and then 9 again e The records will be stored in one lot In order to change the logging lot see the measured unit Setup for details Log option New Lot generation LOGGING MODE 5 and Ae will be available in Measure mode Notes For 4222 set Set and Ae Ae will be available in multichannel Measure mode depending on the focused channel f the Reading Mode option is set as Direct and the Logging Mode 5 session is started a warning pop up will be displayed on the LCD informing the user that the Reading Mode option must be set as Direct AutoHold in order to use this logging mode To log data using this mode Press while in Measure mode to start the logging session The logged values are only the ones Log frozen on the LCD after 5 was pressed and the stability criterion reached To store another frozen value press to return to normal
50. been calibrated before taking pH measurements DIRECT MEASUREMENT To measure the pH of a sample using the Direct reading mode Note If the reading is out of range Press and then select pH Measure mode for the selected channel HI 4222 only Select the Direct reading mode see pH Setup for details page 20 Submerge the electrode tip and the temperature probe approximately 4 cm 1 into the sample to be tested Allow time for the electrode to stabilize The measured pH value will be displayed on the LCD together with a short GLP information or Not Calibrated message if no pH calibration was performed will be displayed on the LCD DIRECT AUTOHOLD MEASUREMENT To measure pH of a sample using the Direct AutoHold reading mode for the selected channel HI 4222 only Select the Direct AutoHold reading mode see pH Setup for details page 20 Submerge the electrode tip and the temperature probe approximately 4 cm 1 7 into the sample to be tested Allow time for the electrode to stabilize The measured pH value will be displayed on the LCD If pressing m The AutoHold indicator will start blinking on the LCD until the stability criterion is reached The pH value will be frozen on the LCD along with AutoHold indicator 42 the buffer this message appears when calibration is performed in one of the previous calibrated buffers 15 02 21 Jan 18 2005 Channel 1 Measure
51. calibration has to be performed 10 58 51 To dear Calibration pH Setup Channel 1 Press while in pH Measure mode Manual Selection Paint Press si e Press and Use A or lear Calibration option Are uou sure you want to e Press see fo clear calibration A pop up menu will be clear the calibration displayed asking for confirmation e Press ve 10 confirm or we 10 escape without saving and return to the Calibration options 24 Sample ID This option allows the user to give to the measured samples an identification number name Two Sample ID options are available ID Increment Mode and Edit Sample ID ID Increment Mode Two increment modes for the sample ID can be selected None the sample ID will be fixed and it can be set alphanumerically Automatic the sample ID will be increased with 1 from the set value for each new log lot To set the ID Increment Mode NUTUS e Press while in pH Measure mode Channel ID Increment Edit Sample ID Press alin it Sample e Us A or v to select the Sample ID option ml open m Edit Sample ID This option allows the user to edit the sample ID Note If the ID Increment Mode is set to None the sample ID can be set alphanumerically otherwise it can be set only numerically To edit the Sample D pH Setup oc Channel 1 Press while in pH Measure mode D Increment Edit Sample ID Press s gt
52. cing power of the tested sample To correctly perform a redox measurement the surtace of the ORP electrode must be clean and smooth DIRECT MEASUREMENT To measure the mV of a sample using the Direct reading mode Press and then v 10 enter mV Measure mode ESTEE for the selected channel HI 4222 only Select the Direct reading mode see mV Setup for details page 32 Measure Submerge the tip of the ORP electrode 4 cm 1 7 into the b 4 sample to be tested and allow a few seconds for the reading eM mV to stabilize The instrument will display the measured mV value on the LCD Note If the reading is out of range will be displayed NoProbet on the LCD 21 6 Start DIRECT AUTOHOLD MEASUREMENT To measure mV of a sample using the Direc AutoHold reading mode Press move and then v 10 select mV Measure mode for the selected channel HI 4222 only Select the Direct AutoHold reading mode see mV Setup for details page 32 Submerge the tip of the ORP electrode approximately 4 1 5 into the sample to be tested and allow a few seconds tor the reading to stabilize The measured mV value will be displayed on the LCD If Measure pressing 4 the AutoHold indicator will start blinking on the LCD until the stability criterion is reached The mV value will be frozen on the LCD along with AutoHold indicator return to normal Measure mode press
53. ctrode C the standard concentration S the electrode slope determined in a Vues 7 the sample volume previous calibration Vo the standard volume f the stoichiometric ratio between sample _ and standard and V 7 V E V sm Example 1 You have sulfide samples and you are adding The reaction is S 2A4g gt Ag S One mole sulfide sample reacts with 2 moles silver standard f 1 Example 2 You have sulfide samples and you are adding Pb The reaction is S Pb PbS One mole sulfide sample reacts with 1 mole lead standard f 1 Analyte Addition and Analyte Subtraction Analyte Addition and Subtraction are variations of the previous two methods With Analyte Addition sample analyte is added to an ion standard being measured The standard and sample contain the same ion mV are taken before and after the sample addition From the mV the analyte concentration is determined AE S a SAMPLE STD V SAMPLE With Analyte Subtraction sample analyte is added to an ion standard being measured The analyte reacts with the measured ion in a known manner thus removing measured ions from the solution From the change in mV the concentration of the analyte is determined V C supLE y f SAMPLE where Cy the sample concentration AE the difference of potential trom the electrode Crp the standard concentration S the electrode slope determined in a V pre
54. d also be recalibrated whenever No ISE Calibration or ISE Calibration Expired message appears on the LCD in the Reminder messages area Due to electrode conditioning time the electrode must be kept immersed a few seconds to stabilize The user will be guided step by step during calibration with easy to follow messages on the display This will make the calibration a simple and error free procedure PREPARATION Pour small quantities of the standard solutions into clean beakers If possible use plastic beakers to minimize any EMC interterences For accurate calibration and to minimize cross contamination use two beakers for each standard solution One for rinsing the electrode and one for calibration Note For accurate measurements add the appropriate ISAB lonic Strength Adjustment Buffer to the calibration standards CALIBRATION PROCEDURE Up to five point calibration is possible using five memorized standard solutions 0 1 1 10 100 1000 ppm and 5 custom solutions The ISE calibration and measurement can be performed with or without temperature compensation If the femperature compensation option is enabled the isopotential point of the electrode must be set in ISE Setup in order to perform correct concentration measurements Before calibrating make sure that the appropriate Electrode Type has been selected in ISE Setup according to the measured lon compound ISE Calibration screen description IMEEM ISE Calibrat
55. d the Standard Volume of set concentration to the sample The method parameters are also displayed on the LCD 21 Press 10 take the second mV reading When the reading is stable press 10 store the second mV reading The ISE measurement results will be displayed on the LCD ANALYTE ADDITION To measure the concentration of a sample using the Analyte Addition incremental method Select the Analyte Addition method see ISE Setup for details page 33 Press see fo start the measurement The first step of the method will be displayed on the LCD in which the user is notified to add the appropriate ISA Buffer Volume if different from 0 mL to the standard and to immerse the electrode into the Standard Volume of set concentration The method parameters are also displayed on the LCD Press 10 set the method parameters Press sea 10 Select next previous parameter to be edit then and use A v set the desired parameter value Press Make sure that the set parameters values are the used ones and submerge the lon Selective Electrode tip approximately 4 cm into the standard solution Press coninue 10 take the first mV reading will be displayed on the LCD in which the user is notified to add the Sample Volume to the standard solution The method parameters are also displayed on the LCD 52 f pressing a the method parameters can be changed and the sample co
56. d value Press to return to the Alarm options If the Saving automatically Isopotential Point This option allows the user to edit the isopotential point of the electrode used for pH measurements The isopotential point is the mV reading for an electrode at which temperature has no effec on the measurement The ideal electrode has an isopotential point of 0 0 mV and 7 00 pH while an actual electrode tipically deviates slightly from the ideal values If the actual isopotential pH for an electrode is known it can be set by accessing this option The isopotential pH limits low and high will be displayed on the LCD Note If the isopotential point has been modified recalibration must be performed for the respective channel To set the Isopotential Point Press while in pH Measure mode 655 sao Use A Press see and set the desired isopotential pH value using o Vv Escape 10 cancel operation 30 pH Alarm Limits Channel 1 Alarm Low 2 000 Alarm High 20 000 pH Press lt gt to exit in previous screen Press Edit to edit focused entry Press Next gt or lt Previous to select entry Escape EN Previous Isopotential Point Channel 1 Edit the value for isopatential point xa 2 000 pH 20 000 pH Limit Low Limit High Use Up and Down arrows to set value Press lt gt to save current value Press lt
57. e the new temperature value 40 When in mode if pressing after entering calibration and while a custom pH calibration butfer is pH Calibration Ch iE selected a pop up menu will be displayed on the LCD in which the custom buffer and the oe value be ajusted by pressing and then or V keys f 110 sove the value and then 10 Select next previous value to be adjusted PN o EDI When in ATC mode if pressing serve after entering T aele calibration and while a standard pH buffer with a Press Setup to edit the temperature xxx resolution or a custom pH buffer is selected a pop up menu will be disployed on the LCD in which the buffer value be ajusted using A or V Press e to Save the new buffer value f the lutomofi buffer entry type has been selected for the calibration procedure the instrument will automatically select the closest buffer to the measured pH value from the edit buffer group see pH Setup for details page 20 f the Semiautomatic buffer entry type has been selected for the calibration procedure the instrument will automatically select the closest buffers to the measured pH value from all the available buffers and the used buffer value can be selected with Previous CALIBRATION MESSAGES Wrong buffer Please check the buffer this message appears when the difference between the pH reading
58. electric charge is its slope can be manually set by pressing A pop up menu will be on press scape 0 return to the lon Constants menu Note lf an SE calibration was performed and a different lon Selective Electrode is selected standard or custom a warning message appears on the LCD informing the userto perform a new calibration or to select the previous ISE in order to perform accurate measurements Concentration Unit Accessing this option the user can select the desired concentration unit for the measured ion or chemical compound The available concentration units are ppt g L ppm mg L ppb ug L mg mL M mol L mmol L and 9ow v To set the Concentration Unit TIE Press while in SE Measure mode Hacc Reading Mode Biase aoe SEUC T erpperature e Use of v oseledthe Une Pd m ise E Tm y SRM M ccr poc p Log b iL u desired option Alarm E Isopatential Point PA mol L e Press see 10 confirm your selection or press 10 ISE Significant Digits mmol L cancel operation Press Select to choose the unit for concentration measurements Escape Select Sample ID See pH Setup section page 20 Stability Criteria See pH Setup section page 20 Log See pH Setup section page 20 Note The Logging Data Configuration option includes also the lon Constants parameter If you want it to appear in the log reports it must be enabled
59. er e Instruction Manual 4221 and 4222 are supplied with 12 Vdc 230 Vac adapter HI 4221 01 and HI 4222 01 are supplied with 12 Vdc 115 Vac adapter Note Save all packing material until you are sure that the instrument works properly Any defective item must be returned in the original packing with the supplied accessories GENERAL DESCRIPTION HI 4221 and HI 4222 are professional bench meters with color graphic LCD for pH ORP Oxidation Reduction Potential ISE HI 4222 only and Temperature measurements with Calibration Check The display can be configured as a single channel or dual channel display HI 4222 only in various modes Basic information only GLP information Graph mode and Log History mode Each channel can be configured as pH mV Relative mV or ISE HI 4222 only The main features of the instruments are e One HI 4221 or two HI 4222 input channels Manual selection automatic and semiautomatic pH calibration up to five points with standard 1 68 3 00 4 01 6 86 7 01 9 18 10 01 and 12 45 and custom buffers up to 5 custom buffers Manual Selection and Custom Standard ISE calibration up to five points with standard 0 1 1 10 100 1000 ppm and custom solutions up to 5 custom solutions with or without temperature compensation e AutoHold feature to freeze first stable reading on the LCD wo selectable alarm limits Five selectable logging modes Automatic logging with and withou
60. frequently in these cases Incremental Methods Incremental methods are useful for the measurement of samples whose constituents are variable or concentrated Incremental techniques can reduce errors from such variables as temperature viscosity or pH extremes and will provide indirect analysis of ions for which there is no ISE sensor for a direct measurement There are four different incremental methods for sample measurement They are Known Addition Known Subtraction Analyte Addition and Analyte Subtraction 4222 allows the analyst to use these techniques as a simple routine procedure thus eliminating calculations or tables The method once set up can be used for repetitive measurements on multiple samples Known Addition and Known Subtraction With Known addition standard is added to a sample being measured The standard and sample contain the same ion mV are taken before and atter the standard addition From the mV the sample concentration is determined C SAMPLE STD AE S V 10 V SAMPLE With Known subtraction a Known standard is added to an ionic sample being measured The standard reacts with the measured ion in the sample in a known manner thus removing measured ions from the solution From the change in mV the concentration of the sample is determined y C SAMPLE f SAMPLE V 10 95 where the sample concentration AE the difference of potential from the ele
61. he selected channel Select the Direct AutoHold reading mode see ISE Setup for details page 33 49 Add ISAB to the sample solution Submerge the lon Selective Electrode tip and the temperature probe approximately 4 cm 11 into the sample to be tested Allow time for the electrode to stabilize The measured concentration value will be displayed on the LCD If pressing Ave the AutoHold indicator will start blinking on the LCD until the stability criterion is reached The concentration value will be frozen on the LCD along with AutoHold indicator Note If the reading is out of range will be displayed on the LCD KNOWN ADDITION To measure the concentration of a sample using the Known Addition incremental method for the selected channel Select the Known Addition method see ISE Setup for details page 33 Press 39 10 start the measurement The first step of the method will be displayed on the LCD in which the user is notified fo add the appropriate ISA Buffer Volume if different from 0 ml to the sample and to immerse the electrode into the Sample Volume The method parameters are also displayed on the LCD Make sure that the set parameters values are the used ones and submerge the lon Selective Electrode tip approximately 4 cm 1 7 into the sample to be tested Press corine 10 take the first mV reading 50 13 14 48 Jan 18 2005 Channel 1 Measure Last Calibr Jan 18
62. ion Channel 1 Measured Calibration concentration Standard Calibrated Standards Calibrated Standards we Last Calibration Jan 18 2005 12 42 Calibration messages Press Clear Cal gt to clear old calibration Press lt Escape gt to cancel the calibration E Clear Previous Sede Cal Standard Standard 46 Two standard entry types are available ManualSelection and Custom Standard The default option is Manual Selection A To calibrate the instrument using Manual Selection standard entry type e Press cat J If the instrument was calibrated before and calibration was not cleared the old calibration can be cleared by pressing lt t After 10 seconds will longer be available Note It is very important 0 dear calibration history when 0 new electrode is used because most errors and warning messages that appear during calibration depend on calibration history e Add ISAB to all standard solutions mmerse the lon Selective Electrode and the temperature probe approximately 4 m 172 into the less concentrated standard solution and stir gently 1127 4cm Select the used standard solution concentration with or The Please wait message will appear on the LCD until the reading is stable or the concentration is validated Note When in ATC mode if pressing serve 0 pop up menu will be displayed on the LCD in which the concentration value can be
63. kaline perform a five point calibration by selecting five of the available buffers CALIBRATION PROCEDURE Calibration has a choice of 8 memorized buffers pH 1 68 3 00 4 01 6 86 7 01 9 18 10 01 12 45 and up to 5 custom buffers For accurate measurements it is recommended to perform a five point calibration However at least a two point calibration is suggested pH Calibration screen description 115 pH Calibration Channel 1 Measured buffer value 10 011 i 25 3 Calibration buffer 0 010 Calibrated Buffers Last Calibration Jan 18 2005 08 40 Calibration messages Press lt gt to update calibration E Previous m Buffer Buffer 39 Three buffer entry types are available Automatic Semiautomatic and Manual Selection The default option is Manual Selection To calibrate the instrument using Manual Selection buffer entry type Press If the instrument was calibrated before and calibration was not cleared the old calibration can be cleared by pressing es After 10 seconds will no longer be available Note It is very important to clear calibration history when 0 new electrode is used because most errors and warning messages that appear during calibration depend on calibration history Immerse the pH electrode and the temperature probe approximately 4 cm into a buffer solution of your choice pH 1 68 3 00 4 01 6 86 7 01 9 18 10 01 12 45 or a custom
64. lable Automatic the instrument automatically selects the closest buffer to the measured pH value from the edit buffer group Semiautomatic the instrument automatically selects the closest buffers to the measured pH value from all available buffers and you can choose the one used Manual Selection the desired pH buffer is manually selected from all available buffers regardless of measured value To set the Butter Entry Type Sa cea Press while In pH Measure mode Channel 1 Buffer Entry Type Manual Selection Press si rece Set Rerninder Period Press 7 Select mda Use Ns Or V x Clear Calibration TM Entry Tyne option Press se and use A 0 V desired option e Press sese 10 confirm your selection or press Esee to cancel operation Escape see V 2 Ist Cal Point Two options are available for the Ist Cal Point parameter Point and Offset If Point option is selected the slope values adjacent to the calibration points will be reevaluated normal calibration If at least a two point calibration has been performed and an offset correction of the electrode is wanted maintaining the existing slope values perform a one point calibration using the Offset option To set the Ist Cal Point Press while in pH Measure mode Channel EU Buffer Entru Type Manual Selection Press pH 1st Cal Point Setup i Edit Custom Buffers eee genes
65. le combination pH electrode Use flasks HI 1230B 8 mm 7 5mm DIA 0 3 0 29 gt 210 8 25 Plastic body PEI double junction gel filled combination pH electrode Use general field 70 HI 2031B Glass body semimicro conic refillable combination pH electrode Use semisolid products HI 1332B Plastic body PEI double junction refillable combination pH electrode Use general purpose 12 mm 0 5 NEM a e B A 120 mim il HI 1413B Glass body single junction flat tip Viscolene non refillable combination pH electrode Use surface measurement os 71 110 gt 4 3 FC 100B Plastic body PVDF double junction refillable combination pH electrode Use general purpose for food industry 12 mm 0 5 1 P 120 mm gt FC 200B Plastic body PVDF2 open junction conic Viscolene non refillable combination pH electrode Use meat amp cheese FC 210B Glass body double junction Viscolene non refillable combination pH electrode Use milk yogurt 12 mm 0 5 1 gt i m 120 mm gt 4 7 FC 220B Glass body triple ceramic single junction refillable combination pH electrode Use food processing 9 5mm D
66. lease wait pop up message will be displayed Press lt gt to save current value Note For larger logged lots the saving time can be up to several minutes see se A V LOGGING MODE 2 Sampling 00 00 07 Note For HI 4222 the 54 or and will be available in multichannel Measure mode depending on the focused channel To log data using this mode e Press 54 while in Measure mode to start the logging session When the measured value is frozen on the LCD by pressing and the stability criterion is reached the logged value is the one that has been frozen on the LCD until returning normal logging mode by pressing ees 58 The Logging sampling period and AutoHold indicators will Im be displayed on the LCD Note While automatic logging is running the measured unit setup is not available A warning message will be dis played on the LCD if the setup is accessed To store another frozen value press 4 again pH e To stop the logging session press gt 3 Log2 F Last Calibr Jan 18 2005 15 13 Electrode Cond 1 00 Note For 4222 tose will be available in Measure Channel 1 channel To log data using this mode record The Logged indicator will be displayed on the LCD 3960 e The records will be stored in one lot In order to change the logging lot see the measured unit Setup for details Log Last Calibr J
67. ment mode pH mV Rel mV or ISE HI 4222 only To enter Setup System pH mV or ISE and to access Log Recall function To obtain general informations about the selected option operation VIRTUAL KEYS The upper row keys are assigned to the virtual keys placed on the bottom of the LCD which allow you to perform the displayed function depending on the current menu e g B and in Measure mode ET le Note All the virtual keys are assigned to the focused channel HI 4222 only LCD GENERAL DESCRIPTION Measure Selected Time amp Date mode Stability indicator Measured value Measurement unit Temperature Short GLP Last Calibr Jan 18 2005 08 40 compensation information Electrode 1 00 Reminder Temperature messages area Low Data Logging Space value amp unit Start Virtual keys mper Lag SPECIFICATIONS HI 4221 HI 4222 2 000 to 20 000 pH 2000 0 mV RANGE 20 0 to 120 0 C 4 0 to 248 0 F 253 15 to 393 15 K 0 1 pH 0 01 pH 0 001 pH RESOLUTION NEP 1 conc 0 1 conc 0 01 conc 0 001 conc 0 1 C 0 1 F 0 1 0 1 pH 0 01 pH 0 002 pH ILSD ACCURACY 0 2 mV ILSD i 0 5 monovalent ions 1 divalent ions 0 2 C 20 4 F X02 K Relative mV offset range 2000 0 mV H Calibration Up to five point calibration 8 standard buffers available p 1 68 3 00 4 01 6 86 7 01 9 18 10 01 12 4
68. ncentration will be recalculated Press to start another measurement Note Press e af any fime to stop the measurement and return to Measure mode ANALYTE SUBTRACTION To measure the concentration of a sample using the Analyte Subtraction incremental method Press wove and then 10 select SE Measure mode tor the selected channel Select the Analyte Subtraction method see ISE Setup for details page 33 Press S to start the measurement The first step of the method will be displayed on the LCD in which the user is notified to add the appropriate ISA Buffer Volume if different from 0 mL to the standard and to immerse the electrode into the Standard Volume of set concentration The method parameters are also displayed on the LCD e Press a 10 set the method parameters Press net Previous 10 Select next previous parameter to be ei then press ea and use A 0 V 10 set he desired value Press Note For the Analyte Subtraction method the ratio factor between standard and must also be set in order to take accurate measurements Make sure that the set parameters values are the used ones and submerge the lon Selective Electrode tip and 4 cm into the solution When the is stable press store the first mV reading The second step of the method will be displayed on the LCD in which the user is nofified to add the Sample Volume to the standard solution
69. nel 1 Last Calibr Jan 18 2005 13 38 f accessing Graph option while logging the online graph can _ Electrode Cond 1 00 be visualized on the LCD see Display Mode section for details page 11 57 f accessing Log History option while logging last logged data can be visualized on the LCD see Display Mode section for details page 11 e stop the logging session press em MAD os Log Save The Log Save screen will be displayed in which the i Log2 logged lot parameters can be set Press to adjust the log interval and or the log Star Tine Jan 18 2005 Channel 1 Lot name L 14 pH sampling or press see 10 save the current log in m Sampling Press lt Save gt to save the current log time or the log sampling Press j Accept x 10 save the Press lt Set Interval gt ta adjust log interval ar log sampling current value and Use 0 Previous 10 adjust Ix Bud s uml next previous parameter Note The minimum log sampling which can be set is calcu Log Save lated related to the logging time thus the 5000 records lot not be exceeded e g for 50 000 seconds logging time the minimum log sampling will be 10 seconds Stan Time Jan 18 2005 Lot name 1014 e Press es 10 exit log interval edit menu and then Stop Time Jan 18 2005 configuration While the instrument is saving the data a P
70. nually selected from a standard solutions list 0 1 ppm 1 ppm 10 ppm 100 ppm and 1000 ppm Custom Standard when the calibration is started each standard solution can be manually set in ppm units in 0 displayed pop up menu To set the Standard Entry Type EE eee Press while in SE Measure mode Standard Entry Type Manual Selection Press e Use v to select the Calibration Hen Press s and use A jor v Standard Entry Type option Press ses 000108 A 0 V e Press Select to 0 nfirm your selection Or press Escape x to mode for standard solution concentration onc oem em oe BV Calibration Reminder See Calibration option from pH Setup section page 20 Set Reminder Period See Calibration option from pH Setup section page 20 Clear Calibration See Calibration option from pH Setup section page 20 Electrode Type This option allows the user to select the desired lon Selective Electrode used for measurements from a list Ammonia Bromide Cadmium Calcium Carbon Dioxide Chloride Chlorine Cupric Cyanide Fluoride lodide Lead Nitrate Potassium Silver Sodium Sulfate Sulfide and five custom ISE For the standard ISE it is possible to view the lon constants Name Molar Weight and Electric Charge Slope while for the custom ISE all these constants can be manually set 35 To set the Electrode Type Press while in SE Measure mode Pres
71. of range in the appropriate scale Dry membrane junction Out of order temperature probe Broken or out of order electrode Dirty broken electrode contaminated buffers Only one point calibration has been performed Internal or software error 66 TROUBLESHOOTING GUIDE SOLUTION Soak the electrode tip in HI 7061 solution for 30 minutes and then clean the electrode Clean the electrode Refill with fresh solution for refillable electrodes only Make sure the sample is in the specified range Recalibrate Check the electrolyte level and the general state of the pH ORP or ISE electrode Soak in 70300 Storage solution for at least one hour Replace the probe Replace the electrode Follow displayed instructions Perform at least a two point calibration Restart the instrument using the power switch If the error persists contact your vendor TEMPERATURE CORRELATION FOR pH SENSITIVE GLASS The resistance of glass electrodes partially depends on the temperature The lower the temperature the higher the resistance It takes more time for the reading to stabilize if the resistance is higher In addition the response time will suffer to a greater degree at temperatures below 25 C 77 F J J L CENSO OLI 20 10 0 10 20 30 40 50 60 0 80 90 C Since the resistance of the pH electrode is in the range of 50 200 Mohm the current acr
72. oss the membrane is in the pico Ampere range Large currents can disturb the calibration of the electrode for many hours For these reasons high humidity environments short circuits and static discharges are detrimental to a stable pH reading The pH electrode s life also depends on the temperature If constantly used at high temperatures the electrode lite is drastically reduced Typical Electrode Life Ambient Temperature 3 years 90 C 194 F Less than 4 months 120 C 248 F Less than month Alkaline Error High concentrations of sodium ions interfere with readings in alkaline solutions The pH at which the interference starts to be significant depends upon the composition of the glass This interference is called alkaline error and causes the pH to be underestimated Hanna s glass formulations have the indicated characteristics Sodium lon Correction for the Glass at 20 25 C 68 77 F Concentration 0 1 Mol L Na 1 0 Mol 67 pH BUFFER SOLUTIONS HI 6001 HI 6003 80041 6004 80001 6068 8007L 600 6091 80091 80101 6010 6124 pH 1 679 Buffer Solution 500 mL bottle pH 3 000 Buffer Solution 500 mL bottle pH 4 01 Buffer Solution in FDA approved bottle 500 mL pH 4 010 Buffer Solution 500 mL bottle pH 6 86 Buffer Solution in FDA approved bottle 500 mL pH 6 862 Buffer Solution 500 mL bottle pH 7 01 Buffer
73. pt r save the E can be by pressing a previous value to 1 adjusted a TEMF 1 Use Left and Fight arrows to select which old standard you want to remove 13 08 10 Jan 18 2005 Channel 1 ISE Calibration Concentration E 1 ppm Temperature 27 6 C Press Gecepr to update calibration Eae Edt Nest Previous CALIBRATION MESSAGES Wrong standard solution Please check the standard solution this message appears when the difference between the ppm reading and the value of the selected standard solution concentration ppm is significant If this message is displayed check if you have selected the appropriate calibration standard 48 Wrong new slope Please check the standard solution this message appears if the current slope exceeds slope window 5096 to 12096 of default slope for the corresponding ion charge see ISE Theory section for details page 54 Recalibrate the instrument using fresh standards Wrong old slope Press lt Clear Cal gt to clear old calibration this message appears as a result of an erroneous slope condition Press clear old calibration and restart calibration Difference between standards temperature is too high Press lt Accept gt to update the calibration or clear old calibration this message appears when the difference between two standards temperature is greater than 5 C Recalibrate the instrument while paying attention to the standard
74. r single junction or HI 7082 or HI 8082 for double junction electrodes Allow the electrode to stand upright for 1 hour Follow the Storage Procedure above pH CLEANING PROCEDURE General Soak in Hanna HI 7061 or 8061 General Cleaning Solution for approximately hour Protein Soak in Hanna HI 7073 or HI 8073 Protein Cleaning Solution for 15 minutes e Inorganic Sodk in Hanna 7074 Inorganic Cleaning Solution for 15 minutes il grease Rinse with Hanna 7077 or 8077 Oil and Fat Cleaning Solution IMPORTANT After performing any of the cleaning procedures rinse the electrode thoroughly with distilled water refill the reference chamber with fresh electrolyte not necessary for gel filled electrodes and soak the electrode in HI 70300 or HI 80300 Storage Solution for at least 1 hour before taking measurements 65 SYMPTOMS Slow response excessive drift Readings fluctuate up and down noise The LCD displays during measurements pH mV mV Rel or ISE Out of range in the mV scale The instrument does not work with the temperature probe The meter fails to calibrate or gives faulty readings Explicit warnings are displayed during calibration The electrode condition is not displayed after calibration The instrument does not override the loading process Dirty pH electrode Clogged dirty junction Low electrolyte level refillable electrodes only Out
75. s System Setup Beeper Saving Confirmation Serial Communication Information Are you sure you want to reset the meter to factory defaults The pH Setup menu allows the user to set the parameters associated with pH measurement and calibration These parameters can be set specifically for each channel HI 4222 only The settings will be applied only to the active channel Accessing pH Setup pH Setup Elbe T Press while in Measure mode and then 007 Temperature select pH range for the desired channel Calibration Sample ID Press setup and then 2 10 access pH Setup menu 22 i p i Reading Mode e Use v highlight the desired option pH Resolution The following is a detailed description of the pH Setup option Press Select ta choose the temperature Temperature The temperature has a direct influence on pH This option allows the user to choose the temperature source and units as well as the desired manual temperature for manual temperature compensation mode Temperature Source 4222 only If using a temperature probe Automatic Temperature Compensation will be performed relative to the displayed temperature with the ATC indicator displayed on the LCD For HI 4222 only the ATC option can be selected for Channel 1 or Channel 2 in accordance with the active channel utilizing a probe or for both channels if two temperature
76. s E e Usei E or v to select the Electrode Type option Press sec 000088 A or v toseledthe desired standard ISE or a custom one from the list For SE Setup options For custom ISE For the lon name the Text Editor menu will be displayed on the LCD Enter the desired information by accepting the highlighted character which is added in the text bar E T T AE keys help the user to select the character is also possible to delete the last character by positioning the cursor on the Backspace character and pressing Suet Press Escape 110 return to the lon Constants menu If the Saving Confirmation is BID Hes ves 10 accept the Ces 10 reum to the iiim mode Otherwise the modified option is saved automatically set the appropriate lon molar weight in g mol units ue A o V Run then ad Accept O SOVE 36 jp m Electrode Type Channel 1 Ammonia Press View to display lon parameters Press Select to use the selected electrode perm Ion C onstants E Ion Molar Weight Channel 1 Set the value for lan molar weight g mol Limit Low 0 001 g mol Limit High 1000 000 Use Up and Down arrows to set value Press lt gt to sawe current value Press Escape to exit in previous screen Escape Acces V 10 select the Electric Charge Slope Use 1 55 ectricCharge Slope Or cand then press see Ifthe lon Eem
77. s temperature ISE MEASUREMENTS Make sure the instrument has been calibrated before taking ISE measurements When using one of the incremental methods for measurement and no or only a one point ISE calibration was performed a warning message will be displayed on the LCD notifying the user that at least a two point ISE calibration must be performed For accurate measurements add the appropriate ISAB lonic Strength Adjustment Buffer to both samples and standards if necessary If ISAB is added to the calibration standards it is important to add ISAB also to the samples to be measured DIRECT MEASUREMENT To measure the concentration of a sample using the Direct reading mode Press and then ow 0 select SF Measure mode for the Tne selected channel Select the Direct reading mode see ISE Setup for details page 33 e Add ISAB to the sample solution e Submerge the lon Selective Electrode tip and the temperature 239 probe approximately 4 cm 112 into the sample to be ppm tested Allow time for the electrode to stabilize The measured concentration value will be displayed on the LCD in Last Calibr Jan 18 2005 12 53 the selected units Fluoride TEMA Note If the reading is out of range will be displayed 27 6 c 2 2 e DIRECT AUTOHOLD MEASUREMENT To measure the concentration of a sample using the Direct AutoHold reading mode Press and then ise to select SE Measure mode for t
78. t AutoHold feature Manual logging with or without AutoHold and AutoHold logging mode Up to 100 logging lots Selectable log interval and log sampling features for Automatic logging GLP feature Online and offline graph User friendly interface on large color graphic LCD 240x320 pixels PC interface via RS232 and USB FUNCTIONAL DESCRIPTION HI 4221 DESCRIPTION FRONT PANEL REAR PANEL Liquid Crystal Display LCD Main Keyboard USB connector ON OFF switch Power adapter socket RS232 serial communication connector Temperature probe socket BNC electrode connector for pH ORP measurements Reterence input socket cw N N O WU 2 2 5 HI 4222 DESCRIPTION FRONT PANEL Last Calibr Jan 18 2005 10 27 ATCA Electrode Cond 1000 REAR PANEL Liquid Crystal Display LCD Main Keyboard USB connector ON OFF switch Power adapter socket RS232 serial communication connector Temperature probe socket Channel 2 BNC electrode connector for pH ORP ISE measurements Channel 2 Reference input socket Channel 2 10 Temperature probe socket Channel 1 11 BNC electrode connector for pH ORP ISE measurements Channel 1 12 Reference input socket Channel 1 O amp O WU gt L KEYBOARD DESCRIPTION FUNCTION KEYS CAL To enter exit calibration mode i To select the desired measure
79. the concentration of the ion in the sample Known Subtraction In the Known Subtraction method a standard with a known concentration is added to the sample reacting with the ion to be measured The stoichiometric ratio between standard and sample must be known The ion concentration is then calculated using the difference in mV potential The method is especially usetull when there is no available ISE for the measured ion 33 Analyte Addition This method is similar to the Known Addition method with the difference that an aliquot of sample is added to a standard of known concentration The sample and standard contain the same ion to be measured The ion concentration is then calculated using the difference in mV potential Analyte Subtraction In the Analyte Subtraction method an aliquot of sample is added to a standard of known concentration reacting with the ion to be measured The stoichiometric ratio between standard and sample must be known The ion concentration is then calculated using the difference in mV potential The method is especially usefull when there is no available ISE for the measured ion To set the Reading Mode Press while in SE Measure mode Use v fo select the Reading Mode option desired option Press see 10 confirm your selection or press esee 10 cancel operation Temperature pH Setup section page 20 Notes To access ISE Setup press and 3 11 04 52
80. y Tel 07851 9129 0 Fox 07851 9129 99 Greece Tel 210 823 5192 Fox 210 884 0210 Indonesiq Tel 210 4584 2941 Fox 210 4584 2942 Japan Tel 03 3258 9565 Fax 03 3258 9567 Korea Tel 02 2278 5147 Fax 02 2264 1729 Malaysia Tel 603 5638 9940 Fax 603 5638 9829 Singapore Tel 6296 7118 Fax 6291 6906 South Africa Tel 011 615 6076 Fax 011 615 8582 Taiwan Tel 886 2 2739 3014 Fax 886 2 2739 2983 Thailand Tel 66 2619 0708 Fax 66 2619 0061 United Kingdom Tel 01525 850 855 Fox 01525 853 668 e N USA Tel 401 765 7500 Fox 401 765 7575 For e mail contacts and a complete list of Sales and Technical offices please see www hannainst com
81. ystem Setup menu without changing Sen Meter Information This option provides general information about the instrument serial number each instrument has an unique identification serial number the software version and the factory calibration date and time for mV and temperature 18 Note All the instruments are factory calibrated for mV and temperature After one year following factory calibration the Factory Calibration Due message will appear on the LCD in the Reminder messages area notifying the user that the instrument should be taken to the nearest Hanna Customer Service for factory calibration To view the Meter Information Press while in Measure mode LIE Use or v oseledihe Meter Information option Press see 10 confirm your selection and to view the Meter menu Restore Factory Settings This option allows the user to reset the instrument to the default factory settings To restore the Factory Settings Press while in Measure mode System i Setup Fr Press Setup or press 10 return to the System Setup menu without restoring defaults Press escape 10 return to Measure mode 19 10 52 08 Jan 18 2005 10 51 52 Jan 18 2005 System Setup Beeper Saving Confirmation GLP Data Date amp Time ooo000000 v1 Factory Calibration my Jan 14 2005 08 14 Temp Jan 14 2005 08 47 Press Escape to return Pre identification parameter

Hanna Instruments Thermometer HI 4221 User's Manual

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