GE SE1200 User's Manual
Contents
1. l ment de chauffage et la lampe indicatrice de temp rature lorsque le s cheur surchauffe Le s chage continuera lorsque Pinstrument refroidi 4 un niveau op rationnel Si Pinterrupteur de s curit est fautif c est dire que Pindicateur de temp rature ne s allume pas lorsque le s cheur est r gl au minimum ou au maximum v rifier que l avant et l arri re du s cheur ne sont pas obstru s Si cela se produit plus d une fois par jour veuillez contacter le Service Technique a GE Healthcare e Les pi ces lectriques du g n rateur ne doivent jamais tre mouill es Ne submerger aucune pi ce du s cheur Essuyer tous les r sidus dans le s cheur apr s usage Voir la section Care and Maintenance page 11 pour l entretien e Si l instrument n est pas utilis en conformit avec les recommandations du fabriquant les protections de s curit qui quipent cet appareil peuvent tre rendues in fficaces e Seulement les accessoires et pi ces d tach es approuv s ou fornis par GE Healthcare sont recommand s pour l utilisation l entretien et r paration de cet appareil Operating Instructions Setting up the Dryer 1 Place the dryer in a well ventilated area such as under a fume hood 2 Plug the mains power cord into a properly grounded receptacle The mains power switch operates the fan which circulates air that is either at ambient temperature or heated if the heater is switched on The th2. preventing slippage as the gel shrinks during drying p Open EP Closed Hoefer SE 1200 Easy Breeze Air Gel Dryer 9 10 Drying the Gels 1 Slide each frame assembly into the Easy Breeze dryer driest assembly on top knobs facing up Turn on the power switch and then set the heater temperature switch to the high low or fan only 0 setting Care and Maintenance Cleaning NOTE Never autoclave or heat any component above 55 C After each use wipe the dryer with a damp cloth Clean each frame with mild soap and water rinse thoroughly with water and distilled water and allow to air dry Never use abrasive cleansers on any part Do not expose the frame to solutions or vapors of aromatic or halogenated hydrocarbons ketones esters alcohols over 30 or concentrated acids over 25 Changing the gasket If the gasket on the inner frame becomes inflexible or no longer holds the gel stack tautly remove it and install a new gasket stretching it slightly around three sides and then rolling it into place on the fourth Changing fuses NOTE Fuses protect equipment by disconnecting loads too large for the instrument s circuit design Replace fuses only with identical fuse values 115 V model one F 12A 250V 3AG 230 V model two T 6 3A 250V 5 x 20 mm 1 Caution Set the power switch to off and unplug the power cord before replacing the fuse 2 Insert a small
3. and Su J C Oven drying method for polyacrylamide gel slab packed in a cellophane sandwich Anal Biochem 141 348 350 1984 Laskey R A The use of intensifying screens or organic scintillators for visualizing radioactive molecules resolved by gel electrophoresis In Methods in Enzymology 65 L Grossman and K Moldave eds Academic Press N Y 365 371 1980 Perng G S Rulli R D Wilson D L and Perry G W A comparison of fluorographic methods of detection of 35S labeled proteins in polyacrylamide gels Anal Biochem 173 387 392 1988 13 14 Customer Service Information Technical Service and Repair GE Healthcare offers complete technical support for all our products If you have any questions about how to use this product or would like to arrange to repair it please call or fax your local GE Healthcare representative NOTE Request a copy of the GE Healthcare Health and Safety Declaration Form before returning the item No items can be accepted for servicing or return unless this form is properly completed Ordering Information Easy Breeze Air Gel Dryer Includes two 20 cm x 20 cm gel frames one 20 cm x 20 cm loading platform and 50 cellophane sheets 115 V 230 V Replacement Parts 20 cm x 20 cm frames and platform Cellophane sheets 33 cm x 33 cm Mylar sheets 33 cm x 33 cm Gel drying frame 20 cm x 20 cm Includes inner and outer frames 8 black knobs and one O ring sealing gask
4. frames that are delivered in the same package as the Easy Breeze are preassembled When you order additional SE 1210 Series gel frames you must install the eight knobs that lock the inner frame in place before they can be used Working with Gel Frames That are Not Preassembled Screw the eight black knobs into the outer gel frame until they fit just short of snug See Figure 2 Continue with step 1 of the assembly instructions Working with Preassembled Gel Frames Turn each black knob a one quarter turn to release the inner frame See Figure 3 Gently pop the inner frame out of the outer frame and continue with step 1 of the assembly instructions Place the inner frame on the loading platform with the side labeled UP facing up Immerse a sheet of porous cellophane in distilled water and soak for several minutes Place the fully hydrated sheet over the inner frame on the loading platform Pour 5 10 ml of distilled water onto the sheet Place the gel s on the cellophane Remove all bubbles by carefully pushing them toward the edge of the gel with your gloved finger Pour 5 10 ml of distilled water around the edges of and onto the gel Cover the gel with a fully hydrated sheet of either cellophane or mylar Cellophane Use for fluorography thick gels or to preserve a gel Fully hydrate a sheet of porous cellophane in distilled water and place it over the gel Cellophane does not quench the signal of fluor treated gels M
5. loss of use and other related exposures however caused arising from the faulty and incorrect use of the product Copyright 1998 GE Healthcare All rights reserved No part of this publication may be reproduced stored in a retrieval system or transmitted in any form by any means without permission in written form from the company Renseignements importants d utilization Pour une bonne compr hension et une utilisation en s curit maximale il convient de lire enti rement ce manuel Francais Dans la documentation qui accompagne l instrument un point d exclamation dans un triangle quilat ral a pour but d attirer Pattention de Putilisateur sur des instructions importantes de fonc tionnment ou de maintenance Tous vos commentaires sur ce manuel seront les bienvenus et veuillez les adresser a GE Healthcare Marketing Department 654 Minnesota Street San Francisco CA 94107 USA GE Healthcare se r serve le droit d effectuer des modifications de ces sp cifications sans aucun pr avis Garantie et responsabilit GE Healthcare garantit Putilisateur que le produit livr a subi avec succ s tous les essais pr vus pour s assurer qu il est conforme aux sp cifica tions et normes en vigueur La garantie incluse dans les conditions de livraison n est valable que si le produit a t install et utilis conform ment aux instruc tions fournies par GE Healthcare La soci t GE Healthcare ne sera en aucu
6. Other fluorography agents include EN7HANCE and sodium salicylate For these add 0 5 2 glycerol to the final cold water precipitation step 3 Load the gel onto the drying frame as described on page 8 Thick Gels Gel thickness gt 0 75 mm and acrylamide gt 10 T 1 Fix stain and destain the gel according to your preferred protocol Use a final destaining solution of 7 acetic acid and 5 methanol 2 Final soak solution Coomassie Blue After the final destain soak the gel in a solution of 35 ethanol and 0 5 2 glycerol for 1 2 hours Ethanol dehydrates the gel which minimizes cracking during drying Do not place the gel in the ethanol solution longer than 2 hours as ethanol will begin destaining the gel Silver stain Add 0 5 2 glycerol to the final water rinse NOTE If you soak gels in a solution of ethanol and glycerol such as the preserve solution in the PlusOne Protein Silver Staining Kit sold by GE Healthcare you can dry the gels after a 2 hour soak Fluorography We recommend the fluorographic agent Resolution Follow the manufacturer s instructions with the following exception for the final water soak use a 35 ethanol and 2 glycerol cold water soak instead Other fluorography 3 agents include EN3HANCE and sodium salicylate For these use 1 glycerol in the final cold water soak to minimize gel cracking Load the gel onto the drying frame as described below Assembling the Gel Drying Stack Gel
7. SE 1200 Easy Breeze Air Gel Drying System User Manual GE imagination at work 80 6290 71 SE1200 IM Rev C 8 98 Hoefer is a registered trademark and Easy Breeze is a trademark of GE Healthcare Bio Sciences Inc All other brand or product names are trademarks or registered trademarks of their respective companies or organizations Important User Information Please read this entire manual to fully understand the safe E ng I sh and effective use of this product The exclamation mark within an equilateral tri angle is intended to alert the user to the presence of important operating and maintenance instructions in the literature accompanying the instrument If you have any comments on this manual please send them to us at GE Healthcare Bio Sciences Inc Marketing Department 654 Minnesota Street San Francisco CA 94107 USA GE Healthcare reserves the right to make changes in the specifications without prior notice Warranty and Liability GE Healthcare guarantees that the product delivered has been thoroughly tested to ensure that it meets its published specifications The warranty included in the conditions of delivery is valid only if the product has been installed and used according to the instructions supplied by GE Healthcare GE Healthcare shall in no event be liable for incidental or consequential damages including without limitation lost profits loss of income loss of business oppor tunities
8. a higher percentage of glycerol a longer drying period is required The gels may also be flexible and feel tacky when dry Fluor treated gel e Use cellophane instead of mylar e Use a thinner gel lt 0 75 mm Gel dries too slowly or not at all Gel dries unevenly e Rotate each frame 180 front to back every half hour Check the gel or stack Reduce the amount of glycerol to 0 5 to 2 e Check that you are not using two sheets of Mylar At least one sheet must be cellophane Check the heater Make sure the indicator lamp lights at the low hear or high hear settings If it doesn t light or the heater does not heat the safety switch may be tripped Check that the front and back of the dryer are unobstructed and wait a few minutes for the dryer to cool then resume heating Gel turns cloudy or white Gels gt 0 75 mm thick with acrylamide gt 10 T Reduce the percentage of ethanol to 30 Do not soak the gel for more than 2 hours in the ethanol glycerol dehydration solution After Coomasie Blue staining be sure to use final destain of 7 acetic acid and 5 methanol Dryer does not function e Check that the power switch is on e Check that the power receptacle is properly grounded and in working condition Check the fuses and replace if necessary e Ifthe dryer overheats make sure air can flow freely by checking that both the front and back are not obstructed If the dryer overheats more than once in the same d
9. ay call the GE Healthcare Technical Service Department 12 Visualization Notes Direct Autoradiography Prepare the gel in the usual manner and expose to film as directed with the instructions accompanying the film Exposure times especially for medium energy beta emitters such as 14C and 38 can be quite long For maximum sensitivity of these types of labels use fluorography Fluorography Fluorography intensifies a weak beta emission signal by impregnating the gel with a suitable fluor that converts beta emission from S 14C and 3H into light Drying the gel in cellophane does not quench this signal but for H a very weak beta emitter up to 20 of the signal may be lost If using ENHANCE or sodium salicylate as fluorography agents include 0 5 to 2 glycerol in the cold water treatment prior to drying Dehydration steps are not required for thinner lower percentage gels which are far less prone to cracking If exposed to the maximum drying temperature 54 C these gels may form longer fluor crystals resulting in higher background on X ray exposures NOTE Certain fluorographic agent manufacturers specify temperatures higher than those reached in the Hoefer Easy Breeze e g Autofluor National Diagnostics and Fluoro Hance Research Products International which require up to 80 C to produce an optimum signal This dryer is not recommended for such agents Bibliography Juang R H Chang Y D Sung H Y
10. e dryer overheats Dryer operation will resume once the unit cools to operating temperature If the safety switch is tripped that is the heater indicator lamp is not lit when the dryer is set to the low or high setting make sure both the front and back of the dryer are completely unobstructed If this occurs more than once in the same day call the GE Healthcare Technical Service Department e The electric components in the power section must be kept dry Do not immerse any part of the dryer in water Wipe away all residue from the dryer after each use Refer to the Care and Maintenance page 11 for cleaning instructions e If this equipment is used in a manner not specified by the manufacturer the protection provided by the equipment may be impaired e Only accessories and parts approved or supplied by GE Healthcare may be used for operating maintaining and servicing this product Informations Importantes Mise en Place du S cheur e Afin d assurer une bonne circulation d air placer le s cheur de facon ce que la partie frontale du s cheur et les orifices d a ration l arri re ne soient pas obstru s e Il est recommand d utiliser le s cheur sous une hotte aspirante durant le s chage de gels trait s avec des solvants organiques ou tout autre produits potentiellement dangereux Ne pas placer le s cheur pr s de liquides inflammables S curit e Vinterrupteur interne de s curit teind l
11. enzione nella documentazione allegata al prodotto Si prega di inviare eventuali commenti al presente manuale a GE Healthcare Bio Sciences Inc Marketing Department 654 Minnesota Street San Francisco CA 94107 USA GE Healthcare si riserva il diritto di apportare modifiche ai dati tec nici senza preavviso Garanzia e responsabilit GE Healthcare garantisce che prima della consegna il prodotto stato collaudato a fondo per soddisfare i requisiti specificati La garanzia inclusa nelle condizioni di consegna risulta valida solamente se il prodotto stato instal lato ed utilizzato nel rispetto delle istruzioni fornite da GE Healthcare GE Healthcare non potr essere ritenuta responsabile di incidenti o danni consequenziali inclusi ma non limitati a perdite di profitti mancato guadagno perdite di affari difetti di funzionamento e relative esposizioni dovuti ad un utilizzo non corretto del prodotto Copyright 1998 GE Healthcare Tutti i diritti riservati Nessuna parte della presente pubblicazione pu essere riprodotta conservata in sistemi di gestione dati o trasmessa in alcun forma n per nessuno scopo senza autorizzazione scritta del produttore CONTENTS Air Dryer Function and Description Unpacking Specifications Operating Instructions Setting up the Dryer Preparing the Gel for Drying Thin Gels Thick Gels Assembling the Gel Drying Stacks Working with Gel Frames That are Not Preassembled Working wi
12. et Order loading platform separately O ring sealing gasket 20 cm x 20 cm frame Knobs black Gel loading platform 20 cm x 20 cm Outer gel drying frame and 8 knobs 20 cm x 20 cm Inner gel drying frame 20 cm x 20 cm and one O ring sealing gasket Replacement Parts 20 cm x 25 cm frames and platform Cellophane sheets 33 cm x 38 cm Mylar sheets 33 cm x 38 cm Gel drying frame 20 cm x 25 cm Includes inner and outer frames 8 black knobs and one O ring sealing gasket Order loading platform separately O ring sealing gasket 20 cm x 25 cm frame Knobs black Gel loading platform 20 cm x 25 cm Outer gel drying frame and 8 knobs 20 cm x 25 cm Inner gel drying frame 20 cm x 25 cm and one O ring sealing gasket Fuses 115 V model F 12A 250 V 3 AC 230 V model T 6 3A 250 V 5 X 20 mm Qty 50 25 50 25 Code No 80 6121 61 80 6121 80 80 6121 99 80 6122 18 80 6122 37 80 6122 56 80 6122 75 80 6122 94 80 6123 13 80 6123 32 80 6430 17 80 6429 98 80 6429 22 80 6430 36 80 6122 75 80 6429 41 80 6430 74 80 6430 55 80 6109 26 80 6112 68
13. flat blade screwdriver into the slot on the fuse module depress slightly and turn it 4 turn counterclockwise The spring loaded module cap will loosen and you can then pull the cap fuse holder out 3 Pull the fuse out of its holder and inspect If the fuse element is burned or broken replace the fuse with an identical type If the fuse appears to be intact check it with a multi meter A reading of 1 Q or less indicates the fuse is still usable 4 Insert the fuse into the holder and then insert this assembly back into the unit Seat the module by inserting the screwdriver into the slot pressing gently and turning the cap 4 turn clockwise 5 230 V model Repeat steps 2 to 4 for the second fuse 11 Troubleshooting Gel or cellophane cracks All gels Handle the gel and cellophane with care Fully hydrate the cellophane with distilled water just before use and avoid stretching it during assembly Ifthe cellophane is drying too quickly instead of hydrating it in distilled water soak it in the same glycerol solution used for the gel Always remove all air bubbles before drying Lower the heat setting e Load frame assemblies into the dryer from the top down Avoid dripping onto a partially dried gel Gradient gels or gels gt 0 75 mm thick with acrylamide gt 10 T e Dehydrate the gel in 35 ethanol and 2 glycerol before drying If gels still crack increase the percentage of glycerol up to 5 Note With
14. n cas responsable de tout dommage caus directement ou indirectement par toute utilisation incorrecte ou non approuv e du produit ou d coulant de cette utilisation y compris toute perte de b n fice ou de recettes toute perte de perspectives commerciales tout emp chement d utilisation et tout autre risques ayant un rapport avec l utilisation du produit mais sans aucune limitation quant la nature de ces dommages Copyright 1998 GE Healthcare Tous droits r serv s La reproduction le stockage dans un syst me de r cup ra tion d informations ou la transmission sous quelque forme que ce soit et par quelque moyen que ce soit de la pr sente publication en totalit ou en partie sont strictement interdits sans autorisation pr alable crite de la soci t Informacion importante para el usuario Para comprender el producto y utilizarlo con seg ES pa n ol uridad es necesario leer este manual en su total idad El signo de admiraci n en un tri ngulo equil tero en el manual advi erte al usuario sobre la presencia de instrucciones importantes de operaci n y mantenimiento del aparato Si desearan hacer alg n comentario sobre este manual tengan la amabilidad de remitirlo a GE Healthcare Bio Sciences Inc Marketing Department 654 Minnesota Street San Francisco CA 94107 USA GE Healthcare se reserva el derecho a modificar las especificaciones sin previo aviso Garant a y responsabilidad GE Healthcare garanti
15. ree heat settings are high setting 54 C o ambient temperature low setting 43 C The indicator lamp located next to the heater switch lights up when the low or high setting is chosen Note Unless gel cracking is a recurring problem prevent gels from becoming tacky by using 2 glycerol or less in the gel soaking solution Preparing the Gel for Drying Listed below are recommended procedures to ensure optimum drying results for gels of different thicknesses and acrylamide concentrations Thin gels 0 75 mm or less are recommended for best results This drying method is not recommended for e Agarose gels which are generally too thick e Polyacrylamide gels attached to GelBond film Thin Gels Gel thickness lt 0 75 mm or gel thickness gt 0 75 mm and acrylamide lt 10 T 1 Fix and stain the gels according to your preferred protocol 2 Final soak solution Coomassie Blue The final destaining solution should consist of 7 acetic acid 5 methanol and 0 5 2 glycerol Glycerol maintains the flexibility of the gel making it less likely to crack Equilibrate the gel in this solution for a minimum of 2 hours but preferably overnight After destaining rinse the gel with distilled water for 1 2 minutes to remove excess acetic acid Silver stain Add 0 5 2 glycerol to the final water rinse Fluorography We recommend the fluorography agent Resolution Follow the manufacturer s instructions
16. sota Street San Francisco CA 94107 USA GE Healthcare beh lt sich das Recht vor die Spezifikationen ohne vorhergehende Ank ndigung zu ndern Gew hrleistung and Haftung GE Healthcare garantiert da das gelieferte Produkt sorgf ltig auf die Einhaltung der ver ffentlichten Spezifikationen getestet wurde Die in den Lieferbedingungen n her erl uterten Gew hrleistungsanspr che gelten nur dann wenn das Produkt gem den von GE Healthcare gelieferten Anweisungen installiert und benutzt wurde GE Healthcare bernimmt keinerlei Haftung f r Sch den oder Folgesch den einschlie lich aber nicht begrenzt auf Gewinneinbufen Einkom mensverluste entgangene Gesch ftsabschl sse Verlust der Gebrauchsf higkeit oder andere Verluste die wie auch immer durch eine fehlerhafte oder unsach gem e Verwendung des Produkts verursacht wurden Copyright 1998 GE Healthcare Alle Rechte vorbehalten Die vorliegende Ver ffentlichung darf nur mit vorherge hender schriftlicher Genehmigung durch das Unternehmen vervielf ltigt in einem Abrufsystem gespeichert oder in irgendeiner Form oder mit irgendwelchen Mit teln bertragen werden Informazioni importanti per operatore Per un utilizzo sicuro del prodotto leggere attentam ente l intero contenuto del presente manuale Italiano Il punto esclamativo all interno di un tri angolo equilatero indica all operatore la presenza di importanti istruzioni di funzionamento e manut
17. th Preassembled Gel Frames Drying the Gels Care and Maintenance Troubleshooting Visualization Notes Direct Autoradiography Fluorography Bibliography Customer Service Information Technical Service and Repair Ordering Information 00 CO NN ND O FW O mu md QQ N O Ha w w _ _ A O Rh e gt A Figure 1 Main components of the SE1200 Easy Breeze Air Dryer Function and Description The SE 1200 Easy Breeze Air Gel Dryer dries polyacrylamide gels by circulating air around gels that are supported on at least one side by semi permeable cellophane The dryer circulates air that is either at ambient temperature or heated to 43 C low setting or 54 C high setting Two 20 cm x 20 cm drying frames and a loading platform 20 cm x 20 cm are included The dryer can hold up to six frames and when fully loaded the dryer can accommodate up to six 20 x 20 cm or twenty four 8 x 10 cm gels With the 20 cm x 25 cm accessory frames the dryer can accommodate up to six 20 cm x 25 cm or thirty six 8 cm x 10 cm gels Heater power switch High Off 0 Mains power switch turns on the fan Slide assembled frames into the racks starting at the top slot Always place the wettest assemblies in the bottom slots Unpacking Unwrap all packages carefully and compare contents with the packing list making sure all items arrived If any part is missing contact your local sales office Inspect all componen
18. ts for damage that may have occurred while the unit was in transit If any part appears damaged contact the carrier immediately Be sure to keep all packing material for damage claims or for repacking should it become necessary to return the unit Specifications 115 V model Max wattage Max voltage Frequency 230 V model Max wattage Max voltage Frequency Environmental operating conditions Installation category Pollution degree Dimensions Product certifications 1325 W 105 125 V 57 63 Hz 1325 W 210 250 V 47 63 Hz Indoor use 4 40 C Humidity up to 80 Altitude up to 2000 m Il 2 width x depth x height 32 x 51 x 27 cm 12 5 x 20 x 10 5 in EN61010 1 UL3101 1 CSA C22 2 1010 1 CE This declaration of conformity is only valid for the instrument when it is e used in laboratory locations and e used as delivered from GE Healthcare except for alterations described in the User Manual Important information Dryer Placement e To ensure enough air flow position the dryer so that both the front of the dryer and air vents in the back are completely unobstructed e It is advisable to run the dryer under a fume hood when drying gels treated with organic solvents or any other potentially hazardous material e Do not place the dryer near flammable liquids Dryer Safety e The dryer s internal safety switch shuts off the heating element and the heater indicator lamp when th
19. ylar Use for contact autoradiography for gels lt 1 mm Fully hydrate a sheet of Mylar in distilled water and place it over the gel Once the gel is dried the Mylar peels off Place the uncovered side against the X ray film IMPORTANT Remove all bubbles trapped under the top sheet by displacing them with distilled water and pushing them to the edge of the gel with your gloved finger Work from the center of a single gel or if several gels are on the platform work from the center of the frame toward the edge Position the outer frame with the Hoefer label facing up and the locking knobs facing down over the cellophane sheets and around the inner frame Press down to engage the two frames Applying pressure to opposite corners of the frame usually causes the frames to slip easily into place Operating Instructions Figure 2 Frame parts and gel drying stack assembly frame size 20 cm x 20 cm or 20 cm x 25 cm Figure 3 Lock the frame Flip the assembly over and turn the knobs perpendicular to the frames Outer frame Hoefer label faces up wai Screw 8 _ into the 3 threaded holes lt in the outer L frame i Orient the frame so that the screws face down ME i i ge TS Inner frame E N with word UP facing up K AS Loading platform _ A 6 Turn the frame assembly over Turn each knob turn so that the long edge is perpendicular to both frames See Figure 3 This locks the two frames together
20. za que el producto entregado ha sido pro bado a fondo para comprobar el cumplimiento de las especificaciones publicadas La garant a incluida en las condiciones de entrega s lo es v lida si el producto se ha instalado y utilizado de acuerdo con las instrucciones entregadas por GE Healthcare GE Healthcare no ser responsable bajo ning n concepto de da os directos o indirectos incluyendo sin limitaci n la p rdida de beneficios la p rdida de ingresos la p rdida de oportunidades de negocio la p rdida de utiliza ci n y otras consecuencias relacionadas cualquiera que sea la causa que se deban a la utilizaci n defectuosa e incorrecta del producto Copyright 1998 GE Healthcare Reservados todos los derechos No est permitida la reproducci n ni el almacenaje en un sistema de recuperaci n ni la transmisi n de parte alguna de esta publicaci n sin la autorizaci n por escrito de la empresa Wichtige Benutzerinformationen F r ein vollst ndiges Verst ndnis und eine sichere Handhabung dieses Produktes ist es notwendig da der Benutzer dieses Handbuch vollst ndig durchliest Deutsch Ein Ausrufezeichen in einem gleichseitigen Dreieck soll den Benutzer auf die Anwesenheit wichtiger Betriebs und Wartungsanweisungen in der dem Ger t beiliegenden Dokumentation hinweisen Wenn Sie Anmerkungen zu diesem Handbuch haben dann senden Sie diese bitte an GE Healthcare Bio Sciences Inc Marketing Department 654 Minne
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