Fujifilm LAS-3OOO User's Manual
Contents
1. illuminator at iris 2 8 without any filter is recommended For digitizing the molecular weight standard on blotted membrane white light epi illuminator which is located in the center part of the blue green and red LED epi illuminator unit is used NP Tray CHEMI LUMINESGUEN CE PLUORLSCGCENCESCHEMIEIFLUOR ES CEN GE Easy to use operation Samples are placed on the appropriate tray and placed in the intelligent dark box After closing the door all the functions are controlled remotely from the Image Reader software The software automatically rec ognizes the type of illuminator installed or changed during operation Users are able to control the LAS 3000 with the Image Reader software in either the user friendly Lite mode or the more advanced Pro mode The modes are selected with the click of a mouse Super CCD By rotating pixels 45 degrees to form an interwoven layout the Super CCDs pixel pitch in the horizontal and vertical directions is narrower than in the The five filter options available for the LAS 3000 diagonal direction achieving higher hori zontal and vertical resolution Quantitative image analysis function The 16 bit signal accumulated on each CCD pixel is processed through an A D converter to form a digital image The image data obtained at the closed shutter condition is the dark frame The image data of an even flat sample is the flat frame To analyze the ima2. size the same as the Standard e Long exposure times are required to capture images of extremely low light samples The low noise design of the LAS 3000 system includes default settings for a two hour exposure and a 15 hour exposure in addition to manual settings for exposures as long as 30 hours e A non parallax NP tray is used to elimi nate the parallax effect when imaging chemiluminescence in the 96 well plate Up to two plates can be placed More versatility for fluorescent detection More and more fluorescent dyes are intro duced into the biochemistry and molecular biology fields for staining proteins and nucleic acids in gels membranes and well plates Expression analysis using green fluo rescent protein GFP and red fluorescent protein RFP can be detected by blue exci tation or green excitation respectively The LAS 3000 includes multiple LED options for various fluorescence applications including blue LED 470nm green LED 520nm and red LED 630nm for epi illu mination and a UV 312nm light source for DIA illumination The five position filter turret holds 77mm filters such as Y515 general purpose for blue LED excitation 510DF10 GFP etc 575DF20 RFP Cy 3 Pro Q Diamond etc R670 Cy 3 ALEXA Fluor 633 etc and 605DF40 EtBr etc Digitizing functions Capturing images as we see them is called digitizing For CBB stained gel or silver stained gel use of the white LED DIA
3. W UV light SYBR Green amp Il SYPRO Orange Chemiluminescence Mode GFP DY 458XL blue LED Pro Q Diamond Cy 3 RFP DY 520XL green LED Alexa Fluor 633 Fluorescence Cy 5 DY 647 red LED E Chemifluorescence Chemifluorescence AttoPhos Digitization CBB stained gel Silver stained gel Transilluminator oe Te Neen NBT BCIP stained membrane MW marker Epi illuminator UV trans b green LED Intelligent Dark Box IV a SS EPI illuminator for fluorescence blue LED 470nm green LED 520nm red LED 630nm nem tumrescence Voce EPI illuminator for digitization White light source Transilluminator for digitization of Digitization Stained gels and autoradiographic films White LED UV transilluminator UV light source 312nm o Filter turret Five positions R ee Printers illuminator Pictrography 3500 Lens lane Analysis Digitize Mode High sensitivity lens FUJINON VRF43LMD Wide view lens F number 0 85 F number 2 0 TE Focal length 43mm Focal length 24mm S C ence ve g A g S y r i em Focus Remote power focusing Focus Manual ibe irene E EE O E Mount Bayonet Mount Adapter to Nikon F mount chemiluminescence fluorescence chemifluorescence and digitization No license is granted for use of AttoPhos to detect nucleic acid on a nylon membrane No license is granted for pre labeling gel electrophoresis method with the UV transilluminator j 7 z s Quality system FUJIFILM Life Sc
4. Specifications and Applications a ae ra Saal Unit Camera Head and Controller a Fj FUJIFILM Science CCD chip Fujifilm Super CCD Area Type chip sorait viy Re sorution Ie l Imaging amp Information Number of pixels 3 2M pixels Pixel size 10 75 x 10 75um Cooling Two stage thermoelectric module with air circulation Cooling temperature 30 C when room is below 28 C Focusing Power focusing remote and preset control Exposure time 1 100 second to 30 hrs 2 hrs to 30 hrs to be set manually Dynamic range Four orders of magnitude Gradation 16 bits 65 536 Image size 12 6MB Max 49 2KB Min Maximum sample size 25 x 25cm wide angle lens 14 x 21cm Fujinon VRF43LMD lens Binning 1x2 2x4 4x8 and 8 x 16 pixels Interface USB1 1 Software The binning mode of the LAS 3000 allows researchers to select from four S U er D Image capture Fujifilm Image Reader Mac and Windows SUE a a a a Image analysis Fujifilm Image Gauge Mac Fujifilm MultiGauge Windows Remote Controlled Dimensions and Weights Sample is placed on a tray Camera head 180 W x 170 H x 250mm D 3 4kg Dark Box IV 510 W x 730 H x 480mm D 49 0kg L Chemiluminescence Analyzing Unit Operating system Windows XP or Mac OS 9 amp X o Applicable Reagents and or Samples Chemiluminescence CDP Star ECL ECL Plus SuperSignal ImmunoStar CSPD Fluorescence EtBr
5. ge data the dark frame and flat frame data are subtracted from the original image This correc tion function is included in the Image Reader software MultiGauge software for Windows and ImageGauge software for Mac are the standard image analysis software included in the ScienceLab software provided by Fujifilm LED units DIGIFIZATIrON Fujifilm Super CCD Area Type imaging chip FUJINON lens VRF43LMD and five position filter turret Applications Fluorescence Chemifluorescence Blue SYBR Green I amp Il SYPRO Ruby SYPRO Tangerine FAM EGEP DY 458XL Green SYPRO Red TAMRA BERA Alexa Fluor 546 Pro Q Diamond BODIPY 576 589 R phycoerythrin HNPP DY 547 Red Alexa Fluor 633 Alexa Fluor 647 BODIPY 650 665 TONOS DY 647 UV Ethidium Bromide White Silver stained NBT BCIP Chemiluminecence ECEN ECL Advance Super Signal CSPD Bright Star SYBR Gold SYPRO Orange EME ECER AttoPhos Cyn ROM Alexa Fluor 532 Deep Purple Rhodamine Red NED RER DY 520XL Alexa Fluor 635 Cy 5 DiD DDAO phosphate SYPRO Rose CBB X ray film ECL Plus Lumi Light Plus CDP Star Renaissance
6. ience TOV i J PRODUCT SERVICE SO 9001 i Y Iel Imaging amp Information Fuji Photo Film Co Ltd 26 30 Nishiazabu 2 Chome Minato ku Tokyo 106 8620 Japan Tel 81 3 3406 2201 Fax 81 3 3406 2158 http lifescience fujifilm com E mail sginfo tokyo fujifilm co jp Fujifilm Medical Systems U S A Inc 419 West Avenue Stamford CT 06902 U S A Tel 1 203 324 2000 ext 6112 1 800 431 1850 ext 6112 in the U S Fax 1 203 351 4713 http www fujimed com E mail SSG fujimed com Fuji Photo Film Europe GmbH Heesenstr 31 40549 Dusseldorf Germany Tel 49 211 5089 174 Fax 49 211 5089 139 http www fujifilm de E mail mkaling fujifilmeurope de LAS 3BO00U Ref No BB 204E 04 09 Specifications and system configuration subject to change for improvement without notice All other product names mentioned herein are the trademarks of their respective owners GFP expression in tobacco leaf Detection of EPO as doping substance in sports Isoelectric profiles of natural EPO found in normal human urine B and the bands found in urine after injections of epoetin or darbepoetin Data courtesy of Dr Francoise Lasne Laboratoire National de D pistage du Dopage Chatenay Malabry France e LUMINESCENT IMAGE ANALYZER LAS FOR APPLICATIONS REQUIRING HIGH SENSITIVITY AND A WIDE RANGE OF FLUORESCENT CAPABILITIES The LAS 3000 imaging system combines Fujifilm s high sensitivity Super CCD came
7. ra technology with the added ver satility of white blue green and red EPI illuminators The Super CCD imaging chip binning mode and specially designed camera lens allow researchers to capture faint light luminescent images with unprecedented sensitivity and resolution Multicolor illuminator options enlarge application area in fluorescent imaging More sensitivity for chemiluminescent detection Western blotting Southern blotting and Northern blotting detection by chemilumi nescence is a widely accepted method The use of a cooled CCD camera system enables the generation of a digital image and quanti tative analysis of the image s signal strength Several state of the art technologies were incorporated to make the LAS 3000 system as sensitive as the conventional film method e The newly designed FO 85 high sensitiv ity Fujinon lens gathers as much light as possible onto the CCD e The CCD is a 3 2M pixel Super CCD having large octagonal shaped CCD pixels 10 75 x 10 75ym in size The CCD is cooled to 30 C by peltier when the environment is below 28 C e Pixel binning in the LAS 3000 electroni cally increases the pixel area to increase sensitivity The binning mode includes four binning levels 1 x 2 Standard 2 x 4 High 4 x 8 Super and 8 x 16 Ultra In the Ultra mode the exposure time is nearly 60 times faster than Standard An image smoothing function increases the pixels to make the image
Download Pdf Manuals
Related Search