PerkinElmer Perkin Elmer - lambda 20 - Perkin Elmer Uvvis-spectrophotometer Lam
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1. Baseline linearity Baseline noise Technical Data Transmission 0 to 100 Absorbance 6 000 to 6 000 display range 1 to 9999 concentration units Absorbance 0 003 measured at Absorbance 1 at 440 nm 546 1 nm and 630 nm with NIST 930 filters Absorbance 0 015 measured at Absorbance 1 at 257 nm and 350 nm with potassium dichromate solution Transmission lt 0 02 at 220 nm 340 nm and 370 nm Absorbance gt 2 measured at 200 nm with potassium chloride solution against distilled water nm slit Absorbance 0 001 2nm slit Absorbance 0 0005 corrected 200 nm to 100 nm scan speed 240 nm min smooth 2 nm slit Absorbance 0 00008 RMS Absorbance 0 0003 peak to peak 3 min at absorbance 0 wavelength 500 nm and response 2 s B K Cr O 60 06 ug mL 0 5 in sulfuric acid c H SO4 0 005 mol L t B KC1 12 mg mL 40 594 85 Lambda 20 Lambda 40 UV Vis Spectrometers Baseline stability Absorbance 0 0003 per hour drift 500 nm after warmup Data Output Digital port One RS 232 C interface serial for connecting a printer or PC optional second RS 232 C interface Display Information parameters and entries are shown on a two line vacuum fluorescence display with 20 alphanumeric characters per line Methods Timedrive Scan Wavelength Program Concentration Analysis with factor and with Calibration 86 Lambda 40 General Dimensions T2. Electrical hazard To prevent potential injury to yourself and damage to the instrument switch OFF all instruments in the system and disconnect them from the line power supply before you alter or make any new electrical WARNING connections Installation Overview NOTE When connecting the temperature sensor align the red mark on the plug with the red mark on the socket Pull the collar on the plug back to connect disconnect the plug Release the collar to secure the plug Connector on Spectrometer Temp J10 Installation See temperature sensor description Cables Lead through the tube ports sample Compartment Cover Unchanged 44 Accessory Installation Multi sippers Electrical hazard To prevent potential injury to yourself and damage to the instrument switch OFF all instruments in the system and disconnect them from the line power supply before you alter or make any new electrical WARNING connections Installation Overview Connector on Spectrometer Accessory Port Installation See sipper manual sample Compartment Cover Unchanged Spectrometer Multi sipper B0167808 Figure 9 Multi sipper electrical connection 45 Lambda 20 Lambda 40 UV Vis Spectrometers Autosampler Electrical hazard To prevent potential injury to yourself and damage to the instrument switch OFF all instruments in the system and disconnect them from the line power supply before you alter or make
3. Please refer to Technical Data on page 83 for the electrical ratings of the spectrometer e The standard sample compartment baseplates have drain holes in them to run off spilled liquids to the benchtop underneath the instrument You can place a sheet of thick filter paper under the instrument if required 26 cm Front view Right side view Figure 4 Space Requirements 24 Basic Installation Unpacking and Inspection l Unpack the components carefully Keep the packing materials for possible future storage or reshipment Examine the components for any signs of damage in shipment In the event of damage or missing parts file an immediate claim with the authorized carrier and inform your PerkinElmer office or representative After the instrument has been unpacked check the exterior and interior for possible damage as follows l DA 6 I Check the entire outer cabinet of the spectrometer for damage Make sure that terminals fuse holders etc are not damaged Open the sample compartment cover checking that it moves freely without binding The compartment must be free of dust or other foreign matter Close the sample compartment cover Open the lamp compartment as described in Halogen Lamp Replacement on page 56 Check for damages or loose cables Close the lamp compartment NOTE We recommend that the initial installation of the spectrometer should be performed by a PerkinElmer service re
4. 71 Lambda 20 Lambda 40 UV Vis Spectrometers Fuses and Cables 10 3 15 A slow blow B0155573 10 6 3 A slow blow B0155576 l l Single Cell Holder B0505071 Ribbon for internal printer 72 System Description D System Description System Description Features Keyboard and Lamp compartment Display Cover Power switch Sample compartment Connector panel Figure 16 Lambda 20 40 Spectrometer Features Optical System Lambda 20 The Lambda 20 UV Vis Spectrometer features an all reflecting optical system The optical components are coated with silica for durability A holographic grating is used in the monochromator The optical system 1s depicted schematically in Figure 17 The monochromator 1s a holographic concave grating with 1053 lines mm in the center Two radiation sources a deuterium lamp and a halogen lamp cover the working wavelength range of the spectrometer 75 Lambda 20 Lambda 40 UV Vis Spectrometers 76 For operation in the visible Vis range mirror MI reflects the radiation from the halogen lamp onto source mirror M2 At the same time MI blocks the radiation from the deuterium lamp For operation in the ultraviolet UV range mirror M1 is raised to permit radiation from the deuterium lamp to strike source mirror M2 Source change is automatic during monochromator slewing Radiation from the source lamp is reflected from source mirror M2 through an optical filter on the filter
5. You must clean the sample compartment every time anything s spilled into it This preserves the matt black finish and prevents corrosion and contamination The standard sample compartment baseplates have drain holes in them to run off spilled liquids to the benchtop underneath the instrument You can place a sheet of thick filter paper under the instrument if required First remove the cell holder or other sample handling accessory from the sample compartment 4 Maintenance Using a soft cloth and mild laboratory detergent solution lightly scrub away all foreign material Using a clean cloth dampened with water rinse the cleaned surfaces thoroughly Dry with lint free cloth or tissue Sample Compartment Window Windows are provided with the spectrometer The window 1s made of silica and may be used in the entire spectral range of the spectrometer The window seals the sample compartment and thus protects the instrument s optics from dust and fuming or aggressive samples Generally the window should be installed at all times The window is an optical component and requires the same care and handling as cells You can remove the window to clean it The frame 1s magnetic and can be removed by hand Windows are most suitably cleaned by wiping them with a soft cloth moistened with ethanol 53 Lambda 20 Lambda 40 UV Vis Spectrometers 54 Use and Care of Cells Cell Handling A good spectrometer cell 1s
6. proceed as follows l Using the GOTO key slew the monochromator to your measurement wavelength or to 460 nm Call up a method that uses transmission T as the ordinate If necessary change the ordinate mode to transmission Open the sample compartment cover Insert the cell with a low absorbing solvent into the sample cell holder Leave the reference cell holder empty Make horizontal fine alignment to the sample cell holder locking screws and milled posts loosened to obtain the highest possible transmittance reading on the display close sample compartment cover while measuring transmittance Make vertical fine adjustment using the vertical adjustment screw again to obtain the highest possible reading close sample compartment cover while measuring transmittance When you are satisfied with the alignment tighten the milled posts and the locking screws on the cell holder Insert the matching cell with the same low absorbing solvent into the reference cell holder The first cell remains in the sample cell holder Repeat steps 5 to 7 with the reference cell holder but this time obtain the lowest possible transmittance reading on the display This completes the fine alignment procedure 33 Lambda 20 Lambda 40 UV Vis Spectrometers When the cell holder has been aligned once you can take it out and reinstall it without aligning it again Minimum Volume Applications To measure minimum sample vol
7. an optical device forming a part of the optical system of the instrument with which it is used It must be accorded the same careful treatment applied to any optical component Optical faults of a minor nature scratches lint fingermarks etc on the optical surfaces can easily introduce substantial analytical errors You should observe the following list of cell handling rules to prevent analytical errors and to achieve utmost precision Only hold cells by non optical surfaces such as the matt finish surfaces Protect cells from scratches and never permit them to rub against one another or against other hard surfaces Avoid abrasive corrosive or stain producing cleaning agents and make certain that the exposed surfaces of cells are optically clean Always wipe the optical surfaces of cells dry and free of fingermarks using a soft cloth or cleaning tissue just before placing them in the cell holder When measuring cold solutions always bear in mind that condensation can form on the optical surfaces Make certain no bubbles cling to the inner surfaces of the cell particularly when handling cold solutions For maximum precision and accuracy calibrate and test with cells of the same type and always insert cells into the holders with the same orientation Pressure Buildup in Cells If you are using stoppered cells observe the following rules to prevent the buildup of internal pressure that could cause the cell to burst M
8. and requires little maintenance other than to keep it clean and free of dust To protect the optical system from dust and fumes you should keep the sample compartment cover closed except for when you are carrying out work in the compartment The sample compartment windows should always be installed 51 Lambda 20 Lambda 40 UV Vis Spectrometers 52 You should observe the following care routine to maintain your instrument n good condition CAUTION Immediately clean all spilled materials from the affected area and wipe it dry with lintless paper or cloth If you have to wipe the sample compartment windows make sure you do not introduce scratches sample windows are optical components and you should handle them in the sampe way as high quality cells Do not leave samples particularly those given to fuming or evaporation in the sample compartment for longer than necessary If any type of sample handling system is installed and portions of it are left in the sample compartment such as a sipper and flowcell make certain that the system 1s cleaned at the end of the working day Generally such systems should be filled with deionized water when left overnight Risk of damage to Optics or Electronics Take care not to spill liquids onto the spectrometer Expensive damage can result to the optics or electronics if liquids are spilled and run inside the instrument or onto the keyboard Cleaning the Sample Compartment
9. connect the printer to the second RS 232 interface make sure the Instrument Configuration method 1s set correctly in Super User 5 Switch on the spectrometer and the printer to activate the new printer configuration 6 Make sure the method PRINTER CONFIG is set correctly for the connected printer in Super User 35 Lambda 20 Lambda 40 UV Vis Spectrometers 36 Spectrometer Printer Printer Cable Figure 8 Connecting an External Printer to the Parallel Port Configuring an External Printer Make sure the spectrometer is correctly configured for your printer type as described in Super User printer configuration in the Operation handbook With Epson printers make sure the printer parameter 1s configured correctly for the type of paper you are using Z fold paper or single sheet Accessory A Installation Accessory Installation Accessory Installation General To operate the spectrometer with some accessories for example the Peltier Temperature sensor you need an accessory printed circuit board PCB and a connector panel They will be installed by PerkinElmer service Procedures for installing the accessories in the spectrometer are described in the directions provided with the respective accessory To install certain accessories you need to remove the sample compartment cover The required procedures are described below In the directions provided with some sample handling accessories refe
10. essere ancora presente all interno dello strumento e Quando lo strumento collegato alla rete di alimentazione i terminali possono essere sotto tensione aprendo le calotte di protezione o rimuovendo alcune parti ad eccezione di quelle raggiungibili con la mano 6 possibile esporre altre parti sotto tensione e Anche se lo strumento stato scollegato da tutte le fonti di tensione i condensatori al suo interno possono essere ancora carichi CI Nello strumento sono presenti tensioni mortali Gevaarlijke spanningen in het instrument gevaar van letsel CNL e Ook in uitgeschakelde toestand kan wanneer het instrument op de netvoeding is aangesloten op sommige plaatsen in het instrument netspanning staan e Ook bij een uitgeschakeld instrument en een onderbroken netvoeding kunnen condensatoren in het instrument nog een gevaarlijke lading bevatten Tensao perigosa no aparelho perigo de ferimento CP e Mesmo desligado o aparelho podera ainda ter tens o de rede em alguns pontos enquanto estiver ligado rede de corrente e Mesmo com o aparelho desligado e a ligac o rede de corrente interrompida os condensadores dentro do aparelho ainda poder o ter ums tensao perigosa aplicada 99 Lambda 20 Lambda 40 UV Vis Spectrometers W02 03 Electrical Hazard A To prevent potential injury to yourself and damage to the instrument switch OFF all instruments in the system and disconnect them from the
11. materials are toxic you may have to treat the collected effluent as hazardous waste Refer to your local safety regulations for proper disposal procedures 13 Lambda 20 Lambda 40 UV Vis Spectrometers Deuterium lamps and other spectral lamps are maintained under reduced pressure When you dispose of lamps that are defective or otherwise unusable handle them correctly to minimize the implosion risk UV Radiation You should be aware of the health hazards presented by ultraviolet radiation e When the deuterium UV lamp is illuminated do not open the spectrophotometer covers unless specifically instructed to do so in the manual e Always wear UV absorbing eye protection when the deuterium lamp is exposed e Never gaze into the deuterium lamp Symbols Used on the Instrument Warning symbol shown on the spectrometer housing Figure 1 Lambda 20 40 Spectrometers 14 KO RE Pt WARNING A WARNING Safety Information UV Radiation Risk of Eye Damage The lamp emits intense UV radiation which can damage your eyes Do not gaze into a lighted lamp Always wear UV absorbing safety glasses when looking at the radiation from the lamp Hot Surfaces Risk of Burns Lamps soon become hot and also heat up other components in the lamp compartment Allow the components to cool to room temperature before installing new lamps or making adjustments in the lamp compartment Warning Labels on the Instrument Wa
12. passes through a convex lens onto the photodiode detector Mirror M5 focuses the radiation beam in the reference cell The beam then passes through a convex lens onto the photodiode detector Optical System Lambda 40 The optical system is depicted schematically in Figure 18 The Lambda 40 UV Vis Spectrometer features an all reflecting optical system The optical components are coated with silica for durability A holographic grating is used in the monochromator The monochromator is a holographic concave grating with 1053 lines mm in the center Two radiation sources a deuterium lamp and a halogen lamp cover the working wavelength range of the spectrometer For operation in the visible Vis range mirror MI reflects the radiation from the halogen lamp onto source mirror M2 At the same time MI blocks the radiation from the deuterium lamp For operation in the ultraviolet UV range mirror MI is raised to permit radiation from the deuterium lamp to strike source mirror M2 Source change is automatic during monochromator slewing Radiation from the source lamp is reflected from source mirror M2 through an optical filter on the filter wheel assembly A stepping motor drives the filter wheel to be in sychronization with the monochromator Depending on the wavelength being produced the appropriate optical filter 1s located in the beam path to prefilter the radiation before it enters the monochromator Filter change is automat
13. questo Warning Waarschuwing Betekent dat wanneer de genoemde aanwijzing niet in acht wordt genomen dit kan leiden tot verwondingen van de gebruiker Warning Aviso Significa que a n o observ ncia da instruc o referida poder causar um ferimento ao usu rio 93 Lambda 20 Lambda 40 UV Vis Spectrometers 94 Caution 00 0 00080 We use the term CAUTION to inform you about situations that could result in serious damage to the instrument or other equipment Details about these circumstances are in a box like this one Caution Achtung Bedeutet da die genannte Anleitung genau befolgt werden mu um einen Ger teschaden zu vermeiden Caution Bem rk Dette betyder at den n vnte vejledning skal overholdes n je for at undg en beskadigelse af apparatet Caution Advertencia Utilizamos el t rmino CAUTION ADVERTENCIA para advertir sobre situaciones que pueden provocar aver as graves en este equipo o en otros En recuadros ste se proporciona informaci n sobre este tipo de circunstancias Caution Attention Nous utilisons le terme CAUTION ATTENTION pour signaler les situations susceptibles de provoquer de graves d t riorations de l instrument ou d autre mat riel Les d tails sur ces circonstances figurent dans un encadr semblable celui ci Caution Attenzione Con il termine CAUTION ATTENZIONE vengono segnalate situazioni che potrebbero arrecare gravi danni allo strumento
14. range see Super User Factory Configuration Scan speeds 7 5 15 30 60 120 240 480 960 1920 and 2880 nm min Ordinate Photometric range Photometric accuracy Stray radiation Baseline linearity Transmission 0 to 100 Absorbance 6 000 to 6 000 display range 1 to 9999 concentration units Absorbance 0 003 measured at Absorbance 1 at 440 nm 546 1 nm and 635 nm with NIST 930 filters Absorbance 40 015 measured at Absorbance 1 at 257 nm and 350 nm with potassium dichromate solution Transmission lt 0 02 at 220 nm 340 nm and 370 nm Absorbance gt 2 measured at 200 nm with potassium chloride solution against distilled water 2nmslit Absorbance 40 0005 corrected 200 nm to 100 nm scan speed 240 nm min smooth 2 B K Cr O 60 06 ug mL 0 5 in sulfuric acid c H SO4 0 005 mol L t B KC1 12 mg mL 0 5 89 Lambda 20 Lambda 40 UV Vis Spectrometers Baseline noise 2 nm slit Absorbance 0 00006 RMS Absorbance 0 0002 peak to peak 3 min at absorbance 0 wavelength 500 nm and response 2 s Baseline stability Absorbance 0 0003 per hour drift 500 nm after warmup Data Output Digital port One RS 232 C interface serial for connecting a printer or PC optional second RS 232 C interface Display Information parameters and entries are shown on a two line vacuum fluorescence display with 20 alphanumeric characters per line Methods Time
15. the deuterium lamp replacement procedure Lamp Energy Attenuator An attenuator 1s located in the lamp compartment between the deuterium lamp and the beam entrance slit If required you can decrease or increase the energy by placing the attenuator into or taking the attenuator out of the beam The attenuator is set at the factory normally in the out position 62 Maintenance Electrical Hazard Switch off the spectrometer and remove the plug from the electrical supply before starting with the replacement 7 Risk of Burns WARNING dZfthe old lamp was lighted allow it to cool before proceeding with the replacement UV Radiation The lamps emit intense radiation which can damage your eyes Do not open the lamp compartment when the lamps are on Do not gaze into a lighted lamp Operate the attenuator as follows 1 Open the lamp compartment 2 Remove the lamp baffle by slackening the thumbscrew for the deuterium lamp and lifting the lamp baffle vertically upward Lamp baffle Thumbscrew deuterium lamp 3 Loosen the thumbscrew holding the attenuator in place 63 Lambda 20 Lambda 40 UV Vis Spectrometers 64 Ta 8 Deuterium lamp assembly Lamp energy attenuator Slide the attenuator downwards into the beam Tighten the thumbscrew Follow the above procedure in reverse to slide the attenuator out of the beam Replace the lamp baffle using the reverse of the procedure described in ste
16. thumbscrew for the deuterium lamp and lifting the lamp baffle vertically upward 60 Maintenance Lamp baffle Thumbscrew deuterium lamp 4 Unplug the deuterium lamp connector from the terminal board by squeezing in the two lugs at each side of the connector and carefully pulling the connector vertically upward Deuterium lamp connector Deuterium lamp assembly 5 Remove the lamp assembly from the bracket by slackening the thumbscrew and pulling the lamp mount vertically upward save the thumbscrew for use with the new lamp assembly 61 Lambda 20 Lambda 40 UV Vis Spectrometers 6 Unpack the new lamp assembly taking care to hold it only by the metal mount to prevent fingermarks on the lamp window 7 Slip the slot at the base of the lamp mount over the stud on the bracket in the lamp compartment and then secure with the thumbscrew 8 Plug the deuterium lamp connector into the socket NOTE The socket in the lamp compartment is asymmetric the deuterium lamp connector can be inserted in one direction only Make certain that the connector is the right way round before inserting it Never attempt to insert the connector by force 9 Wipe the lamp window with a soft cloth moistened with alcohol to remove dirt since this would otherwise be burned in when the lamp is hot 10 Replace the lamp baffle using the reverse of the procedure described in step 3 11 Replace the lamp compartment cover This completes
17. 05 Safety Information 1 Safety Information Safety Information Safety Information in the Handbook This handbook contains information and warnings that must be followed by the user to ensure safe operation and to maintain the instrument in a safe condition Possible hazards that could harm the user or result in damage to the instrument are clearly stated at appropriate places throughout this handbook The following safety conventions are used throughout this handbook We use the term WARNING to inform you about situations that could result in personal injury to yourself or other persons Details about these circumstances are in a box like this one WARNING We use the term CAUTION to inform you about situations that could result in serious damage to the instrument or other equipment CAUTION Details about these circumstances are in a box like this one Translations of the warning messages used in this handbook are given in Translations of Warnings on page 93 Before using the instrument it 1s essential to read the manual carefully and to pay particular attention to any advice concerning potential hazards that may arise from the use of the instrument The advice is intended to supplement not supercede the normal safety code of behavior prevailing in the user s country Lambda 20 Lambda 40 UV Vis Spectrometers IEC 1010 Compliance This instrument has been designed and tested in accordance with IEC 1010 1 Safety requ
18. Lambda 20 Lambda 40 Installation and Maintenance Guide Release History Part Number Publication Date 09935055 November 1996 B July 2000 User Assistance PerkinElmer Ltd Post Office Lane Beaconsfield Buckinghamshire HP9 1QA Printed in the United Kingdom Notices The information contained in this document is subject to change without notice PerkinElmer makes no warranty of any kind with regard to the material including but not limited to the implied warranties of merchantability and fitness for a particular purpose PerkinElmer shall not be liable for errors contained herein for incidental consequential damages in connection with furnishing performance or use of this material Copyright Information This document contains proprietary information that is protected by copyright All rights are reserved No part of this publication may be reproduced in any form whatsoever or translated into any language without the prior written permission of PerkinElmer Inc Copyright 2000 PerkinElmer Inc Trademarks Registered names trademarks etc used in this document even when not specifically marked as such are protected by law UV WinLab is a trademark of PerkinElmer Inc PerkinElmer is a registered trademark of PerkinElmer Inc Contents Contents rosis 3 cI Infor Mal iii cas 5 Sale St EE 7 Safety Information in the Handbook sss 7 IEC TOTO EE E 8 CSA R needed 8 UE Coin pane e ecco ue M IL M MM M E E 8 Elec
19. WARNING line power supply before you alter or make any new electrical connections Gef hrdung durch Elektrizit t Um Verletzungsgefahr durch elektrische Spannung und Besch digungsge fahr der Ger te zu vermeiden schalten Sie alle Ger te des MeBplatzes AUS und trennen Sie die Netzverbindungen bevor Sie irgendwelche elektrischen Verbindungen herstellen oder ndern Fare p grund af elektricitet For at undg faren for kveestelser fra den elektriske speending og faren for beskadigelse af apparaterne skal alle apparater p m lepladsen veere SLUKKET og stramtorbindelserne skal vaere skilt inden De opretter eller ndrer noget i de elektriske forbindelser amp Para evitar posibles da os personales y al equipo apague todos los m dulos del sistema y descon ctelos de la toma de corriente antes de establecer nuevas conexiones el ctricas o de modificar conexiones Peligro el ctrico existentes Risque d lectrocution Afin d viter des blessures ventuelles et des d t riorations de l instrument mettre tous les instruments du syst me hors tension et les d connecter de l alimentation par le secteur avant de modifier des connexions lectriques ou d en r aliser de nouvelles Pericolo elettricit Per evitare potenziali incidenti alle persone e danni allo strumento mettere in posizione OFF l interruttore di tutti gli strumenti del sistema e scollegarli dalla fonte di alimentazione prima di modificare i colle
20. aintenance e Only fill the cell so full that the liquid meniscus is just above the radiation beam The remaining air space in the cell 1s then adequate to compensate for any slight increase in pressure in the cell during routine operation e If for analytical reasons it is necessary to fill the cell completely insert the stopper only lightly so that the liquid in the cell has a chance to expand e Do not insert a stopper forcefully into a completely filled cell since this is likely to cause the cell to burst e When working at higher temperatures use a drilled stopper 0 4 mm hole to allow for expansion in the cell Replacing a Lamp Lamp compartment Figure 11 Lamp compartment 55 Lambda 20 Lambda 40 UV Vis Spectrometers 56 White cerami connector Halogen lamp AR assembly Deuterium lamp connector A x 56 bd Deuterium lamp assembly Lamp energy attenuator Figure 12 Inside the Lamp Compartment Baffle removed Halogen Lamp Replacement If the lamp burns out or if the bulb becomes blackened after prolonged use you should replace the lamp Replacement lamp assemblies are provided complete with prealigned mounts Part Number B0114620 Maintenance Figure 13 Prealigned Halogen Lamp B0114620 WARNING Electrical Hazard Switch off the spectrometer and remove the plug from the electrical supply before starting with the replacement Risk of Burns If the old lamp was lighted
21. allow it to cool before proceeding with the replacement UV Radiation The lamps emit intense radiation which can damage your eyes Do not open the lamp compartment when the lamps are on Do not gaze into a lighted lamp Switch off the spectrometer and unplug the line power cord Remove the lamp compartment cover by pressing down the catch and pushing the cover to the left Remove the lamp baffle by slackening the thumbscrew for the deuterium lamp and lifting the lamp baffle vertically upward 57 Lambda 20 Lambda 40 UV Vis Spectrometers Lamp baffle Le er cr Thumbscrew deuterium lamp 4 Carefully pull the white ceramic connector from the rear of the halogen lamp White ceramic connector Ecke ee RN Orc Thumbscrew Halogen lamp assembly 58 10 IT Maintenance Remove the lamp assembly from the bracket by slackening the thumbscrew and pulling the lamp mount vertically upward Save the thumbscrew for use with the new lamp assembly Unpack the new lamp assembly taking care to hold 1t only by the metal mount to prevent fingermarks on the bulb Slip the slot at the base of the lamp mount over the stud on the bracket in the lamp compartment and then secure with the thumbscrew Carefully push the ceramic connector firmly onto the pins on the base of the lamp Wipe the bulb with a soft cloth moistened with alcohol to remove dirt since this would otherwise be burned in when
22. angerous International interruption is prohibited WARNING Lethal voltages are present in the instrument e Even with the power switch OFF line power voltages can still be present within the instrument When the instrument is connected to line power terminals may be live and opening covers or removing parts except those to which access can be gained without the use of a tool is likely to expose live parts Capacitors inside the instrument may still be charged even if the instrument has been disconnected from all voltage sources When working with the instrument e Connect the instrument to a correctly installed line power outlet that has a protective conductor earth ground e Do not attempt to make internal adjustments ore replacements except as directed in this handbook e Do not operate the instrument with any covers or parts removed Lambda 20 Lambda 40 UV Vis Spectrometers 10 Servicing should be carried out only by a PerkinElmer service representative or similarly authorized and trained person Disconnect the instrument from all voltage sources before opening it for any adjustment replacement maintenance or repair If afterwards the opened instrument must be operated for further adjustment maintenance or repair this must only be done by a skilled person who is aware of the hazard involved Use only fuses with the required current rating and of the specified type for replacement Do not use make
23. any new electrical WARNING connections Installation Overview Autosampler B0509311 Figure 10 Autosampler electrical connection Spectrometer 46 Accessory Installation Personal Computer You can connect a personal computer PC to the spectrometer v a the Serial Port RS 232 port Make sure that the Serial Port RS 232 port is configured for use with a PC default see Super User method RS232 PORT CONFIG To operate the spectrometer v a the PC you additionally require a PerkinElmer UV software package for example UV WinLab PECSS Ask your PerkinElmer service representative for more details 47 Lambda 20 Lambda 40 UV Vis Spectrometers 48 Maintenance 5 Maintenance Maintenance Obtaining Service All internal servicing of the instrument should be performed by a PerkinElmer service representative or similarly authorized person Please contact your local PerkinElmer sales or service office to obtain service Maintenance procedures that you can perform yourself are described in this chapter Unauthorized Adjustments and Servicing Do not attempt to make adjustments replacements or repairs to this instrument except as described in the accompanying User Documentation Only a PerkinElmer service representative or similarly trained and authorized person should be permitted to service the instrument WARNING Daily Care The instrument is constructed with high quality components
24. arts 71 Replacing a Lamp 55 S safety information Electromagnetic Compatibility EMC 10 safety Information 7 CSA Compliance 8 Electrical Protection 8 Electrical Safety 8 Environment 12 Graphic Symbols Used on the Instrument 14 IEC 1010 Compliance 8 UL Compliance 8 UV Radiation 14 Setting up the Printer 35 Connecting an External Printer 35 single Cell Holder 29 Aligning 31 Fine Alignment 33 Installing 30 Minimum Volume Applications 34 System Configuration 27 System Description 75 Features 75 Optical System Lambda 20 75 Optical System Lambda 40 78 T Technical Data 83 Lambda 20 83 Lambda 40 87 Temperature Sensor 44 Translations of Warnings 93 U Unpacking and Inspection 25 Use and Care of Cells 54 W Warnings Translation 93
25. be legible Center Point Tube Ports 30 Basic Installation 3 Move the milled posts a little to locate the threaded holes n the baseplate and then tighten the milled posts The tube ports located at the front of the sample compartment allow you to lead tubes from flowcells water thermsotatted cell holders etc in and out of the sample compartment When not n use you should always insert the caps into the ports Aligning the Single Cell Holder Coarse alignment of the single cell holder is carried out as follows l Open the sample compartment cover 2 Fill matching cells with a low absorbing solvent deionized water or ethanol 3 Insert one cell into the sample cell holder and one into the reference cell holder Make certain that the cell is pushed down fully NOTE 7e alignment procedure is for a given cell in a given holder After alignment the cell should always be used in the same holder 4 Block the sample and reference beam window on the right hand side of the sample compartment with a card to prevent white light from saturating the detector 5 Return to standby display 6 Using the GOTO key slew the monochromator to 0 nm to obtain a beam of visible zero order radiation in the sample compartment 7 By holding a piece of matt white paper behind each cell holder visually examine the light spot to see that the radiation beam is passing through the cell sample area Diffraction patterns bec
26. ctrometers 102 Index 1 U Index A Accessory Connections 41 Accessory Connector Panel 39 Accessory Installation 39 Autosampler 46 General accessory installation 39 Linear Transporter 43 Multi sippers 45 Personal Computer 47 Temperature Sensor 44 Autosampler 46 B Basic Installation 23 Connecting to the Electrical Supply 28 Equipment Provided 26 Laboratory Requirements 23 Overview basic installation 23 Printer 35 single Cell Holder 29 System Configuration 27 Unpacking and Inspection 25 C Cleaning the Sample Compartment 52 Connecting up the Printer Configuring an External Printer 36 Index D Deuterium Lamp Replacement 59 F Fuses Changing 65 H Halogen Lamp Replacement 56 I Internal Printer Changing the Printer Paper 66 Changing the Printer Ribbon 67 Maintaining 66 L Lambda 20 Optical system 75 Technical data 83 Lambda 40 Optical system 78 Technical data 87 Lamp Alignment 64 Lamp Energy Attenuator 62 Linear Transporter 43 M Maintenance 51 Changing Fuses 65 105 Lambda 20 Lambda 40 UV Vis Spectrometers 106 Cleaning the Sample Compartment 52 Daily Care 51 Maintaining the Internal Printer 66 Obtaining Service 51 Replacing a Lamp 55 Use and Care of Cells 54 Multi sippers 45 P Personal Computer 47 Preface 19 Documentation 20 Preparing the Working Area 23 Pressure Buildup in Cells 54 R Replacement P
27. drive Scan Wavelength Program Concentration Analysis with factor and with Calibration 90 Translations of Warnings Translations of Warnings Translations of Warnings This annex contains translations of the warnings used in this handbook WARNING 900 0 08 WO1 01 Warning We use the term WARNING to inform you about situations that could result in personal injury to yourself or other persons Details about these circumstances are in a box like this one Warning Warnung Bedeutet daB es bei Nichtbeachten der genannten Anweisung zu einer Verletzung des Benutzers kommen kann Warning Advarsel Betyder at brugeren kan blive kveestet hvis anvisningen ikke overholdes Warning Peligro Utilizamos el t rmino WARNING PELIGRO para informarle sobre situaciones que pueden provocar dafios personales a usted o a otras personas En los recuadros como ste se proporciona informaci n sobre este tipo de circunstancias Warning Danger Nous utilisons la formule WARNING DANGER pour avertir des situations pouvant occasionner des dommages corporels l utilisateur ou d autres personnes Les d tails sur ces circonstances sont donn es dans un encadr semblable celui ci Warning Pericolo Con il termine WARNING PERICOLO vengono segnalate situazioni che potrebbero provocare incidenti alle persone Troverete informazioni su tali circostanze in un riquadro come
28. e limits of a Class A digital device pursuant to Part 15 of the FCC Rules These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instruction manual may cause harmful interference to radio communications Operation of this equipment in a residential area 1s likely to cause harmful interference in which case the user will be required to correct the interference at his own expense Changes or modifications not expressly approved by the manufacturer could void the user s authority to operate the equipment 11 Lambda 20 Lambda 40 UV Vis Spectrometers 12 WARNING Environment Operating Conditions Explosive Atmosphere This instrument is not designed for operation in an explosive atmosphere The instrument will operate correctly under the following conditions e Indoors e Ambient temperature 15 C to 35 C e Ambient relative humidity 20 to 80 without condensation e Altitude in the range 0 m to 2000 m Storage Conditions You can store the instrument safely under the following conditions e Indoors e Ambient temperature 15 C to 35 C e Ambient relative humidity 20 to 80 without condensation e Altitude in the range 0 m to 2000 m When you remove the instrument from sto
29. echnical Data Scanning double beam spectrometer for the UV Vis range with four selectable slit widths microprocessor and keyboard Width 650 nm Height 260 nm Depth 560 nm Ambient operating temperature E 357 Humidity range Technical Standard Radio interference suppression 20 to 80 relative humidity without condensation In compliance with the requirements for technical instruments stipulated by IEC 1010 1 9 90 In compliance with the legal requirements of the EMC directive 89 336 EEC EN 50 081 1 EN 50 082 1 87 Lambda 20 Lambda 40 UV Vis Spectrometers Optics Beam center height 15 mm above cell holder bottom Beam cross section 0 5 mm sl t ca 0 25 mm x 7 mm width x height Imm slit ca 0 6 mm x 7 5 mm width x height 2mmslit ca 1 mm x 7 5 mm width x height 4mmslit ca2 mm x 7 5 mm width x height at focal point of sample and reference beam in sample compartment Optical pathlength in 121 mm sample compartment Grating Monochromator Holographic concave grating with 1053 lines mm in the center Prealigned deuterium and halogen lamps Detector Photodiodes One for the sample beam and one for the reference beam Abscissa Wavelength range 190 nm to 1100 nm 0 nm for alignment purposes Spectral bandwidth 0 5 nm 1 nm 2 nm 4 nm selectable fixed slit widths 88 Lamp change Technical Data Automatically at 326 nm selectable over the whole wavelength
30. f elektricitet Apparatet skal altid vere jordet Man m under ingen omst ndigheder skille eller fjerne jordlederen inde i apparatet eller i str mledningen Peligro el ctrico Cualquier interrupci n del conductor de protecci n dentro o fuera del aparato o la desconexi n del terminal del mismo toma de tierra podr an ocasionar serios peligros al usar el equipo Prohibida la interrupci n intencionada Risque d lectrocution Toute interruption du conducteur de protection l int rieur ou l ext rieur de l instrument ou d connexion du raccord du conducteur de protection terre peut rendre l instrument dangereux Il est interdit d interrompre volontairement ce conducteur Pericolo elettricit Qualsiasi interruzione della protezione del conduttore all interno o all esterno dello strumento o lo scollegamento del terminale di terra massa del conduttore di protezione possono rendere pericoloso lo strumento E vietato provocare volontariamente queste interruzioni Risico s door elektriciteit Het instrument moet voor de werking altijd geaard zijn In geen geval mag de aarding van het instrument of de netvoeding worden onderbroken of worden verwijderd Perigo por electricidade Para a operag o o aparelho tem de estar sempre ligado a terra De forma alguma separar ou retirar os condutores de protecg o a terra no aparelho ou no cabo de alimentag o da rede 97 Lambda 20 Lambda 40 UV Vi
31. gamenti elettrici o di creare nuovi collegamenti Risico s door elektriciteit Om het gevaar van letsel door elektrische spanning en het gevaar voor beschadiging van het instrument te voorkomen schakelt u voordat u welke elektrische verbinding dan ook wilt maken of wilt veranderen alle instrumenten van de meetplaats UIT en ontkoppelt u de netvoeding Perigo por electricidade A fim de evitar o perigo de ferimento por tensao el ctrica e o perigo de danificag o dos aparelhos DESLIGUE todos os aparelhos do posto de medig o e interrompa as ligacoes rede antes de estabelecer ou modificar qualquer ligag o el ctrica 9000 0 100 Translations of Warnings WG CG UV Radiation Risk of Eye Damage The lamp emits intense UV radiation which can damage your eyes Do not gaze into a lighted lamp Always wear UV absorbing safety glasses when looking at the radiation from the lamp UV Strahlung Gef hrdung der Augen Die Strahlungsquelle Lampe emittiert intensive UV Strahlung und kann dadurch Augensch den verursachen Tragen Sie eine Schutzbrille die die emittierte UV Strahlung ausreichend absorbiert wenn Sie in die leuchtende Strahlungsquelle Lampe schauen Ultraviolet str ling farligt for jnene Str lingskilden lampen emitterer ultraviolet str ling og kan derfor for rsage gjenskader Baer beskyttelsesbriller som absorberer den emitterede ultraviolette str ling tilstreekkeligt nar De se
32. hange the printer paper Change the printer ribbon when necessary Changing the Printer Paper l 2 Lift off and remove the old paper roll Replace with a new paper roll Cut square the end of the paper Carefully feed the end of the paper into the slot at the top of the printer 5 Maintenance Press 4 Print to feed the paper through the printer until it appears at the front Changing the Printer Ribbon i Ze Lift off and remove the paper roll Push in the catch on the printer cover Carefully l ft out the printer Gently pull the ribbon cassette forwards to remove Replace with a new one Replace the printer and printer cover using the reverse of the above procedure 67 Lambda 20 Lambda 40 UV Vis Spectrometers 68 Replacement Parts 6 Replacement Parts Replacement Parts Supplies accessories and replacement parts can be ordered directly from PerkinElmer PE XPRESS PerkinElmer s catalog service offers a full selection of high quality ultraviolet fluorescence and polar metry supplies through the Supplies Catalog for Ultraviolet Visible and Fluorescence Spectroscopy and Polarimetry To place an order request a free catalog or ask for information If you are located within the U S call toll free 1 800 762 402 8 a m to 8 p m EST Your order will be shipped promptly usually within 24 hours If you are located outside of the U S call your local PerkinElmer sales office
33. he sample compartment 3 Carefully slide the cover straight up off both hinges to remove You install the sample compartment cover or other accessory cover by performing this procedure in reverse Sample Compartment Front Cover Some of the accessories used with the spectrometer require removal of the sample compartment front cover Do this carefully as follows l Open the sample compartment cover 2 Carefully slide the cover straight up off both hinges to remove You install the sample compartment cover or other accessory cover by performing this procedure in reverse Sample Compartment Window To remove or install certain accessories you need to remove the sample compartment windows Each window has a magnetic frame and can be easily removed by hand 42 Linear Transporter Electrical hazard To prevent potential injury to yourself and damage to the instrument Accessory Installation switch OFF all instruments in the system and disconnect them from the WARNING connections Installation Overview Connector on Spectrometer Installation Cables Tubes Sample Compartment Cover Alignment line power supply before you alter or make any new electrical Accessory Port See linear transporter handbook Lead through bottom of spectrometer housing Lead through the tube ports See linear transporter handbook Unchanged 43 Lambda 20 Lambda 40 UV Vis Spectrometers Temperature Sensor
34. ializado devidamente formado e autorizado 95 Lambda 20 Lambda 40 UV Vis Spectrometers 96 WO1 03 Explosive Atmosphere This instrument is not designed for operation in an explosive atmosphere E gt z t Explosionsf hige Atmosph ren Das Ger t darf nicht in explosionsf higen Atmosph ren betrieben werden Eksplosive omgivelser Apparatet m ikke anvendes i eksplosive omgivelser Atm sfera explosiva Este aparato no ha sido disenado para utilizarlo en atm sferas explosivas Atmosph re explosive Cet instrument n est pas concu pour fonctionner dans une atmosph re explosive Atmosfera esplosiva Questo strumento non e adatto per l uso in atmosfera esplosiva Explosiegevaarlijke omgevingen Het instrument mag niet in een explosiegevaarlijke omgeving worden gebruikt Atmosferas explod veis O aparelho n o pode ser utilizado em atmosferas explodiveis 96000869 Translations of Warnings c a WO Electrical Hazard Any interruption of the protective conductor inside or outside the instrument or disconnection of the protective conductor earth ground terminal is likely to make the instrument dangerous Intentional interruption is prohibited ae 0 0 0 0 0 Gef hrdung durch Elektrizit t Das Ger t mu zum Betrieb immer geerdet sein Auf keinen Fall die Schutzleiter im Ger t oder in der Netzzuleitung trennen oder entfernen Fare p grund a
35. ic during monochromator slewing From the optical filter the radiation passes through the entrance slit Selectable Slit 1 of the monochromator System Description The radiation is dispersed at the grating to produce a spectrum The rotational position of the grating effectively selects a segment of the spectrum reflecting this segment through the exit slit Selectable Slit 2 to mirror M3 Halogen lamp X M2 Deuterium MT lamp Filter wheel a Selectable Slit 1 M5 A selectable Zeg Slit 2 MA Grating N N Monochromator Figure 18 Optical Path for Lambda 40 M Mirror M1 M4 and M5 Plane Mirror M2 Toroidal Mirror M3 Spherical Mirror Reference Detector tes M3 iam Splitter Lens T3 Detector ample The exit slit restricts the spectrum segment to a near monochromatic radiation beam The slits provide a spectral bandpass of 0 5 nm 1 nm 2 nm and 4 nm From mirror M3 the radiation 1s reflected onto a beam splitter which allows 50 of the radiation to pass onto plane mirror MA and reflects 50 of the radiation onto plane mirror MS Mirror M4 focuses the radiation beam in the sample cell 79 Lambda 20 Lambda 40 UV Vis Spectrometers The beam then passes through a convex lens onto the photodiode detector Mirror M5 focuses the radiation beam in the reference cell The beam then passes through a convex lens onto the photodiode detector 80 Technical Data 8 Techn
36. ical Data Technical Data Lambda 20 General Scanning double beam spectrometer for the UV Vis range with microprocessor and keyboard Dimensions Width 650 nm Height 260 nm Depth 560 nm Ambient operating temperature 15 C to 35 C Humidity range 20 to 80 relative humidity without condensation Technical Standard In compliance with the requirements for technical instruments stipulated by IEC 1010 1 9 90 Radio interference suppression In compliance with the legal requirements of the EMC directive 89 336 EEC EN 50 081 1 EN 50 082 1 83 Lambda 20 Lambda 40 UV Vis Spectrometers Optics Beam center height 15 mm above cell holder bottom Beam cross section Imm slit ca 0 6 mm x 9 mm width x height 2mmslit ca mm x 9 mm width x height at focal point of sample and reference beam in sample compartment Optical pathlength in 121 mm sample compartment Grating Monochromator Holographic concave grating with 1053 lines mm in the center Prealigned deuterium and halogen lamps Detector Photodiodes One for the sample beam and one for the reference beam Abscissa 0 nm for alignment purposes nm or 2 nm fixed slit Lamp change Automatically at 326 nm selectable over the whole wavelength range see Super User Factory Configuration Scan speeds 7 5 15 30 60 120 240 480 960 1920 and 2880 nm min 84 Ordinate Photometric range Photometric accuracy Stray radiation
37. irements for electrical equipment for measurement control and laboratory use and Amendment 1 to this standard CSA Compliance This instrument meets the Canadian Standards Association CSA Standard CAN CSA C22 2 No 1010 1 92 Laboratory Equipment UL Compliance This instrument meets the Underwriter Laboratories UL Standard UL 3101 1 Oct 93 Electrical Equipment for laboratory use part 1 general requirements Electrical Protection Insulation Class I as defined in IEC 1010 1 Installation Category The instruments are able to withstand transient overvoltage according to Installation Category II as defined 1n IEC 1010 1 and IEC 664 Pollution Degree The equipment will operate safely in environments that contain non conductive foreign matter and condensation up to Pollution Degree 2 as defined in IEC 1010 1 and IEC 664 Electrical Safety To ensure satisfactory and safe operation of the instrument it 1s essential that the green yellow lead of the line power cord is connected to true electrical earth ground If any part of the instrument is not installed by a PerkinElmer service representative make sure that the line power plug is wired correctly Safety Information Protective Conductor Green Yellow earth ground Electrical Hazard Any interruption of the protective conductor inside or outside the instrument or disconnection of the protective conductor earth ground terminal is likely to make the instrument d
38. ntes exceptuando aquellos a los cuales se puede acceder con la mano e Los condensadores internos del aparato pueden permanecer cargados incluso cuando el aparato haya sido desconectado del voltaje de la linea Pr sence de tensions mortelles dans l instrument CF e M me lorsque l interrupteur de puissance est sur ARRET des tensions de secteur peuvent encore tre pr sentes dans l instrument e Lorsque l instrument est reli au secteur les raccords peuvent tre sous tension et des parties sous tension peuvent tre d couvertes en ouvrant des capots ou en retirant des pi ces l exception de celles auxquelles il est possible d acc der manuellement e Les condensateurs contenus dans l instrument peuvent encore tre charg s m me si l instrument a t d connect de toutes les sources de tension Translations of Warnings A W02 02 e Even with the power switch OFF line power voltages can still be present T Lethal voltages are present in the instrument within the instrument WARNING e When the instrument is connected to line power terminals may be live and opening covers or removing parts except those to which access can be gained by hand is likely to expose live parts e Capacitors inside the instrument may still be charged even if the instrument has been disconnected from all voltage sources e Anche se l interruttore di alimentazione in posizione OFF la tensione di linea pu
39. o ad altra apparecchiatura l Troverete informazioni su tali circostanze in un riquadro come questo Caution Opgelet Betekent dat de genoemde handleiding nauwkeurig moet worden opgevolgd om beschadiging van het instrument te voorkomen Caution Aten o Significa que a instru o referida tem de ser respeitada para evitar a danifica o do aparelho A WARNING O 000860 Translations of Warnings Unauthorized Adjustments and Servicing Do not attempt to make adjustments replacements or repairs to this instrument except as described in the accompanying User Documentation Only a Perkin Elmer service representative or similarly trained and authorized person should be permitted to service the instrument Unzul ssige Arbeiten am Ger t Wartungs oder Reparaturarbeiten oder Justierungen die in der Benutzer dokumentation zum Ger t nicht beschrieben sind d rfen nur vom Perkin Elmer Kundendienst oder von entsprechend ausgebildeten und autorisierten Fachkr ften ausgef hrt werden Ikke tilladte arbejder p apparatet Vedligeholdelses eller reparationsarbejder eller justeringer som ikke er beskrevet i dokumenterne til brugeren m kun udfores af Perkin Elmers kundeservice eller af tilsvarende uddannede og autoriserede fagfolk Ajustes y servicios sin autorizaci n No intente realizar ning n tipo de ajuste sustituci n o reparaci n en este aparato a excepci n de lo descrito en la Documentaci n del U
40. ome apparent if the radiation beam impinges on the cell wall 31 Lambda 20 Lambda 40 UV Vis Spectrometers 32 8 9 If the radiation beam is not centered exactly loosen the two locking screws and the two milled posts on the relevant cell holder and shift the cell holder plate to center the radiation beam horizontally Then retighten the two milled posts and the two locking screws Now visually check the vertical alignment of the radiation beam in the cell sample area Alignment is correct when the radiation beam is just above the floor of the cell sample area min 2 mm or covers the cell window NOTE 7e center of the window for micro flowcells should be ideally approximately 15 mm above the base of the cell Figure 7 Correct Alignment of the Radiation Beam in the Cell Sample Area 10 11 12 13 If vertical alignment s required turn the vertical adjustment screw on the l fter either clockwise to raise the cell or counterclockwise to lower the cell Recheck the horizontal alignment of the radiation beam through the cell and correct if necessary Using the GOTO key slew the monochromator to any value above 200 nm Remove the card blocking the sample beam window and close the sample compartment cover Basic Installation This completes the coarse alignment of the cell holder If necessary proceed with the fine alignment as described below Fine Alignment If fine alignment is necessary
41. p 2 Close the lamp compartment Lamp Alignment Procedure Due to the prealigned mounts the alignment of lamps after installation is generally so good that further alignment is not required Maintenance Changing Fuses Electrical hazard To prevent potential injury to yourself and damage to the instrument switch OFF all instruments in the system and disconnect them from the d b line power supply before you alter or make any new electrical WARNING connections The fuses are located in a fuse holder at the rear of the instrument Fuse Holder gt Figure 15 Rear view Fuse Holder l Switch off the instrument and remove the line power cord from the electrical supply 2 Squeeze the two lugs at each side of the fuse holder and gently pull out Fuse Holder 65 Lambda 20 Lambda 40 UV Vis Spectrometers 66 3 Replace the spent fuse with a new one of the same type and rating 4 100V 120 V 6 3 A slow blow 210 V 240 V 3 15 A slow blow NOTE The module has two fuses Replace the fuse holder Align the lug at the bottom of the fuse holder with the slot in the socket A click s heard as each lug snaps into place NOTE f you use the correct fuses but the instrument still does not work correctly or the fuses blow repeatedly contact your PerkinElmer office or representative Maintaining the Internal Printer The internal printer needs no special maintenance You only need to C
42. pply via a multisocket distributor Single Cell Holder Locking screw for horizontal alignment Vertical adjustment SCrew Milled post Lifter LAMBDA Locking screw 9 for horizontal v alignment Figure 6 Single Cell Holder B0505071 NOTE Depending on the spectrometer the single cell holder can be installed in two different positions in the sample compartment Always install the holder such that the arrow on the cell holder lines up with the center point on the baseplate see Installing the Cell Holder on page 30 29 Lambda 20 Lambda 40 UV Vis Spectrometers Inscription legible Use in Spectrometer on Holder LAMBDA In this position the cell holder can be used with all Lambda Series Spectrometers BIO LAMBDA 2 In this position the cell holder can be used with Lambda 2 Series Spectrometers as Lambda 10 20 40 Bio etc baseplate with 4 threaded holes The smallest beam diameter is exactly in the middle of the cell This is useful especially for operation with micro and semi micro cells Installing the Cell Holder Install the single cell holder in the sample compartment as follows l Orientate the holder so that the lifter is toward the rear of the sample compartment 2 Lower the holder so that the two alignment holes slip onto the two studs on the baseplate at the bottom of the sample compartment The arrow on the cell holder must line up with the centre point of the baseplate and BIO LAMBDA 2 must
43. presentative After receipt of the instrument please contact your PerkinElmer office or representative for further information You are required to prepare a suitable working space 25 Lambda 20 Lambda 40 UV Vis Spectrometers 26 Equipment Provided Spectrometer 1 Lambda 20 Spectrometer Double beam OR 1 Lambda 40 Spectrometer Double beam with four selectable slit widths according to order Components The components listed below are provided with the spectrometer Quantity Part Number Pack of spare fuses Line power cord with suitable connector for your electrical outlets ER RSR wem nom wem __ MEE EN I eme 0 Basic Installation System Configuration Spectrometer External Printer option Figure 5 Components of the System e You can place the optional printer to either side of the spectrometer to suit your own preferences e Do not place the printer or other instruments on top of the spectrometer e When placing instruments side by side always leave a small gap between them e If you wish you can set up the printer on a separate trolley cart e Make certain that air can circulate freely over and under the system as well as behind it CAUTION Do not place anything on or under any of the components that could hinder free air circulation 27 Lambda 20 Lambda 40 UV Vis Spectrometers Connecting to the Electrical Supply Electrical Hazard To prevent potential inju
44. r ind i den lysende stralingskilde S Radiaci n de rayos ultravioleta La lampara emite una intensa radiaci n de rayos ultravioleta que puede ser perjudicial para los ojos No mire fijamente a la l mpara Al examinar la radiaci n de las l mparas utilice siempre gafas de seguridad que absorban este tipo de rayos Rayonnement UV La lampe met un rayonnement UV intense susceptible de provoquer des l sions oculaires Ne pas regarder fixement une lampe allum e Toujours porter des lunettes de protection absorbant les UV pour regarder le rayonnement des lampes Radiazioni ultravioleite La lampada emette intense radiazioni ultraviolette dannose per gli occhi Non fissare le lampade accese Indossare sempre occhiali protettivi ad assorbimento degli ultra violetti quando si guardano le radiazioni emesse dalla lampade UV straling Risico s voor de ogen De stralingsbron lamp emitteert intensieve UV straling en kan daardoor letsel aan de ogen veroorzaken Draag een veiligheidsbril die de ge mitteerde UV straling voldoende absorbeert wanneer u in de brandende stralingsbron moet kijken Radiacao UV perigo para os olhos A fonte de radiac o l mpada emite uma radiagao UV intensapodendo assim provocar ferimentos aos olhos Use culos de protec o que absorvam suficientemente a radia o UV emitida ao olhar directamente para a fonte de radiag o luminosa 9 00 9 101 Lambda 20 Lambda 40 UV Vis Spe
45. rage before putting it into operation allow it to stand for at least a day under the approved operating conditions Safety Information Chemicals Use store and dispose of chemicals that you require for your analyses in accordance with the manufacturer s recommendations and local safety regulations Hazardous Chemicals Some chemicals used with this instrument may be hazardous or may become hazardous after completion of an analysis WARNING The responsible body for example Laboratory Manager must take the necessary precautions to ensure that the surrounding workplace and instrument operators are not exposed to hazardous levels of toxic substances chemical or biological as defined in the applicable Material Safety Data Sheets MSDS or OSHA ACGIH or COSHH documents Venting for fumes and disposal of waste must be in accordance with all national state and local health and safety regulations and laws OSHA Occupational Safety and Health Administration U S A ACGIH American Conference of Governmental Industrial Hygienists COSHH Control of Substances Hazardous to Health U K Toxic Fumes If you are working with volatile solvents or toxic substances you must provide an efficient laboratory ventilation system to remove vapors that may be produced when you are performing analyses Waste Disposal Waste containers may contain corrosive or organic solutions and small amounts of the substances that were analyzed If these
46. ration Guide This handbook contains comprehensive information on operating the spectrometer and describes the parameters used Basic Installation 3 Basic Installation Basic Installation Overview For spectrometers with installation the initial installation will be performed by a PerkinElmer service representative After receipt of the instrument please contact your PerkinElmer office or representative for further information For spectrometers without installation you can easily install the instrument by following the directions given in the table below If you intend to use accessories for example a cell changer or sipper refer to Accessory Installation on page 39 to install these accessories Preparing the Working Area For maximum stability and minimum maintenance observe the following requirements when siting the instrument e A firm base free from vibration e Enough space around and underneath the instrument for efficient air circulation e A constant temperature between 15 C and 35 C e Constant humidity between 20 and 80 relative humidity e An atmosphere free from dust and corrosive fumes e Keep out of direct sunlight Illumination with diffuse lighting 1s ideal 23 Lambda 20 Lambda 40 UV Vis Spectrometers e A suitable source of electrical power should be located in the vicinity of the instrument Electrical power must be available at a proper earth grounded 3 wire electrical outlet
47. rence is made to earlier models in the Lambda Series of spectrometers These directions are generally applicable to the current series of instruments since the sample compartment is standardized Accessory Connector Panel LP5 5 To facilitate manufacturing procedures a common connector panel is used for the Lambda Series of instruments Not all connectors are required for Lambda 20 or Lambda 40 see table below 39 Lambda 20 Lambda 40 UV Vis Spectrometers aabo o 0000000000000000000 J11 ee 0000000000009000000 ARA o Autosampler J6 Sphere J8 000000 D DOOD O Cell Changer J10 2 RS232 00000 O 0000000006000 00000 00000 000000000000 Accessory Port Serial Port Parallel Port Linear transporter I C bus interface multi sipper PC printer First RS 232 C interface P15 2 RS 232 PC printer Second RS 232 C interface J10 J11 Temp Temperature sensor J10 2 pin socket J11 4 pin socket J30 Peltier Peltier cell changer Active and Peltier cell holder 40 Accessory Installation J88 Sphere Integrating Sphere Accessory Connections Older Accessories Temperature Sensor External Printer Multi sipper PC 41 Lambda 20 Lambda 40 UV Vis Spectrometers Sample Compartment Cover Some of the accessories used with the spectrometer require removal of the sample compartment cover l Do this carefully as follows 2 Open the cover only about 90 degrees to t
48. rning labels shown on the inside of the lamp compartment Figure 2 Lambda 20 40 Spectrometers 15 Lambda 20 Lambda 40 UV Vis Spectrometers The following warnings are shown on the inside of the lamp compartment DANGER DANGER HIGH HAUTE VOLTAGE TENSION WARNING UV RADIATION HARMFUL TO THE EYES HOT COMPONENTS RISK OF BURNS ACHTUNG UV STRAHLUNG GEFAHRDUNG DER AUGEN HEISSE BAUTEILE VERBRENNUNGSGEFAHR ATTENTION RADIATION UV DOMMAGEABLE POUR LES YEUX PARTIES CHAUDES RISQUE DE BRULURES 16 Preface 2 Preface Preface Application The Lambda 20 and Lambda 40 spectrometers are intended for routine UV Vis analys s The spectrometer features a double beam all reflecting optical system Lambda 20 and Lambda 40 spectrometers are usable n a w de range of applications as indicated by their performance specifications see Technical Data on page 83 Lamp ompartment Keyboard and Display Cover Power Switch Space for Optional Printer Sample Compartment Figure 3 Operating controls for Lambda 20 40 Spectrometers 19 Lambda 20 Lambda 40 UV Vis Spectrometers 20 Documentation A documentation package 1s provided with the spectrometer Installation and Maintenance Guide This handbook describes the installation setup and maintenance procedures for the spectrometer It also includes a detailed description of the spectrometer and a list of consumables and replacement parts Ope
49. ry to yourself and damage to the instrument first make the electrical connections between the WARNING i E i instruments in the system before connecting to the line power supply The spectrometer automatically adjusts to the correct operating voltage Before starting the instrument for the first time make sure that the correct fuse is fitted to your line power supply If you have a different fuse change it for the correct one Do not connect the spectrometer to the line power supply if the wrong fuse is fitted 1 Make sure that the correct fuses are fitted in the holder at the rear of the spectrometer see Changing Fuses on page 65 100 V 120 V 6 3 A slow blow 210 V 240 V 3 15 A slow blow NOTE The module has two fuses 2 Make sure that the plug fitted to the line power cord provided with the spectrometer is suitable for your local electrical outlets If it is not remove it and fit a plug conforming to the local regulations 3 After all connections have been made between the various components of the system make certain that all power switches are set to off then connect the line cords to the electrical power supply The power switch is located at the top right hand rear of the spectrometer 28 Basic Installation NOTE To prevent interferences caused by earth loops when operating with ancillary instruments printers etc connect all components of the system to the same phase ofthe electrical su
50. s Spectrometers 98 W02 02 Lethal voltages are present in the instrument e Even with the power switch OFF line power voltages can still be present within the instrument WARNING e When the instrument is connected to line power terminals may be live and opening covers or removing parts except those to which access can be gained by hand is likely to expose live parts e Capacitors inside the instrument may still be charged even if the instrument has been disconnected from all voltage sources Gef hrliche Spannung im Gerat e Auch in ausgeschaltetem Zustand kann an einigen Stellen im Ger t Netzspannung anliegen wenn das Ger t am Stromnetz angeschlossen ist e Auch bei ausgeschaltetem Ger t und getrennter Netzverbindung k nnen Kondensatoren im Ger t noch mit gef hrlicher Spannung geladen sein DK Farlig sp nding i apparatet fare for kv stelser e Ogs i slukket tilstand kan der vere netspaending nogle steder i apparatet hvis apparatet er tilsluttet til str mnettet e Selv n r apparatet er slukket og str mforbindelsen er afbrudt kan kondensatorerne i apparatet v re ladet med farlig sp nding En el aparato existen voltajes letales CE e Incluso con el interruptor desconectado puede haber voltaje dentro del equipo e Cuando el instrumento se encuentre conectado a la red el ctrica los terminales pueden estar bajo corriente y stos quedar expuestos al abrir las cubiertas o al extraer compone
51. shift fuses or short circuit the fuse holders Whenever it is likely that the instrument is no longer electrically safe for use make the instrument inoperative and secure it against any unauthorized or unintentional operation The instrument is likely to be electrically unsafe when it Shows visible damage Fails to perform the intended measurement Has been subjected to prolonged storage under unfavorable conditions Has been subjected to severe transport stresses Electromagnetic Compatibility EMC European Union EMC Directives This instrument has been designed and tested to meet the requirements of the EC Directives 89 336 EEC and 92 3 1 EEC It complies with the generic EMC standards EN 50 081 1 rf emissions and EN 50 082 1 immunity for domestic commercial and light industrial environments Safety Information This instrument has passed the following EMC tests Emission EN 50 081 1 92 Immunity EN 50 082 1 92 Emission of conducted Electromagnetic Compatibility and radiated noise IEC 801 2 91 EN 55011 91 IEC 801 3 84 EN 60 555 2 87 IEC 801 4 88 EN 60 555 3 87 TEC 801 5 90 United States FCC This instrument is classified as a digital device used exclusively as industrial commercial or medical equipment It 1s exempt from the technical standards specified in Part 15 of the FCC Rules and Regulations based on Section 15 103 c Japan FCC This instrument has been tested and found to comply with th
52. suario que se adjunta Tan s lo un ingeniero de Servicio T cnico de Perkin Elmer o una persona de formaci n y autorizaci n similares podr n realizar trabajos de revisi n y mantenimiento delinstrumento R glages et entretien non autoris s Ne pas essayer d effectuer des r glages des remplacements ou des r parations sur cet instrument d une mani re autre que celle d crite dans la Documentation Utilisateur jointe Seul un repr sentant du service apres vente Perkin Elmer ou une personne autoris e et de m me formation devraient tre autoris s intervenir sur l instrument Regolazione e manutenzione non autorizzate Non tentare di regolare sostituire o riparare questo strumento se non come descritto nell allegata Documentazione Utente Solo un rappresentante dell assistenza Perkin Elmer o una persona dalla analoga esperienza pu eseguire se autorizzata la manutenzione dello strumento Niet toegestane werkzaamheden aan het instrument Onderhouds reparatie of afstelwerkzaamheden die niet in de gebruikersdocumentatie van het instrument worden omschreven mogen alleen door personeel van de klantenservice of door overeenkomstig geschoolde en geautoriseerde vakmensen worden uitgevoerd Trabalhos n o permitidos no aparelho Trabalhos de manutenc o ou de reparac o ou ajustamentos n o descritos na documenta o do usu rio para o aparelho apenas podem ser executados pelo Servico T cnico da Perkin Elmer ou por pessoal espec
53. the lamp is hot Replace the lamp baffle using the reverse of the procedure described in step 3 Replace the lamp compartment cover This completes the halogen lamp replacement procedure Deuterium Lamp Replacement If the lamp burns out or indicates falling energy after prolonged use you should replace the lamp Replacement lamp assemblies are provided complete with prealigned mounts Part Number B2000501 59 Lambda 20 Lambda 40 UV Vis Spectrometers i Operating Hours Counter Figure 14 Prealigned Deuterium Lamp Assembly B2000501 NOTE An operating hours counter is incorporated in the red deuterium lamp lead By means of a gap between the two display bars it is possible to read off the number of hours that the lamp has been in operation One scale division corresponds to approximately 100 hours Electrical Hazard Switch off the spectrometer and remove the plug from the electrical supply before starting with the replacement i 7 Risk of Burns WARNING Jf the old lamp was lighted allow it to cool before proceeding with the replacement UV Radiation The lamps emit intense radiation which can damage your eyes Do not open the lamp compartment when the lamps are on Do not gaze into a lighted lamp l Switch off the spectrometer and unplug the line power cord 2 Remove the lamp compartment cover by pressing down the catch and pushing the cover to the left 3 Remove the lamp baffle by slackening the
54. trical Protect Ea 8 ACA a etia Fiet oe 8 Electromagnetic Compatibility EMC sss sse eee eee eee 10 AA A 12 Symbols Used on the Instrument 14 A POP 17 Preta PR 19 O n A N a 19 Documentation 20 Bas c Installation ua aaa Ren 21 Bai on seen een 23 A LT T dati 23 Preparing the Working Arkansas 23 Unpacking and Inspection eb tee inopes e bebe db n cens 25 Eguipment PrOVIOOQ uu eaim o ee 26 System C OM EE E een leihen 27 Connecting to the Electrical Supply 45 oleae 28 sinole Cell Holder zn asien et moa ati a een 29 Minimum Volume Anplcanons 34 Seti up tlie PNET u 35 Accessory INSTALACION EE 37 lb Te 39 ET C 39 An ee 43 Temperature Sensor un rien 44 Ee 45 O O 46 PS sl 47 MEA PP RU 49 Lambda 20 40 Installation amp Maintenance Guide Matte 51 EE ee SI BERNA RE edo ted SI Cleaning the Sample Compartment eee eee eee ee 52 Us and Carol Cells dada 54 Replace no E een 55 A e A 65 Maintaining the Internal Printer eu 66 Replacement Parts TIT TT 69 Replacement PATS anne DEM MEN UP UE 71 System M DESC HIP COM ER 73 SEET 75 Il TT 75 Optical System Lambda 2D ii 75 Optical System Lambda AO uos eese rx ae 78 Technical Data a aka 81 Kee et er RD 83 Lambda EE 83 Lambda EE 87 Translations of ANERE gedeelt 91 Translations Of WM 93 Index EE A O TTE E ES N EE EA 103 lillo 1
55. umes use microcells offered by PerkinElmer The minimum sample volume required is a function of the cell internal width or volume and is specified below Cell Type Cell Pathlength Minimum Internal Volume Number Width Required H liquid slightly pair more than height of 4 mm lcm 400 uL B063 1064 beam pair Cell Pathlength Minimum Part Volume Volume Number Required completely liquid 30 uL 50 uL B0631079 e bg e 34 Basic Installation NOTE You should align microcells very carefully in the radiation beam by following the procedures in Aligning the Single Cell Holder on page 31 When aligning microcells fill each cell with the minimum volume of liquid specified in the above table to make sure that the liquid meniscus is not in the radiation beam Setting up the Printer Activating the Internal Printer If you want to use the internal printer make sure the method PRINTER CONFIG is set to EPSON INTERNAL in Super User On delivery the default setting s EPSON INTERNAL Connecting an External Printer You can use an external printer for hard copy printouts of the analytical results PerkinElmer offers suitable printers refer to the current price list for details To install an external printer l Switch off the spectrometer if it 1s on 2 Setup the printer as given in the User s Guide provided with the printer 3 Configure the printer 4 Connect the printer to the Parallel Port If you
56. wheel assembly A stepping motor drives the filter wheel to be in sychronization with the monochromator Depending on the wavelength being produced the appropriate optical filter 1s located in the beam path to prefilter the radiation before it enters the monochromator Filter change is automatic during monochromator slewing From the optical filter the radiation passes through the entrance slit Slit 1 of the monochromator The radiation is dispersed at the grating to produce a spectrum The rotational position of the grating effectively selects a segment of the spectrum reflecting this segment through the exit slit Slit 2 to mirror M3 Halogen lamp C M2 Deuterium lamp Y Filter wheel M5 Slit 2 Grating MA N Monochromator Figure 17 Optical Path for Lambda 20 System Description M Mirror M1 M4 andM5 Plane Mirror M2 Toroidal Mirror M3 Spherical Mirror Reference a Detector 40 Lens M3 A Beam Splitter Lens Detector Sample The exit sl t restricts the spectrum segment to a near monochromatic radiation beam The slits provide a spectral bandpass of 1 nm or 2 nm From mirror M3 the radiation is reflected onto a beam splitter which allows 50 of the radiation to pass onto plane mirror M4 and reflects 50 of the radiation onto plane mirror MS Mirror M4 focuses the radiation beam in the sample cell 77 Lambda 20 Lambda 40 UV Vis Spectrometers 78 The beam then
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