"取扱説明書"
Contents
1. Transdirect DNA DNA Transdirect secr ce mRNA 3 Octamer binding transcription factor 4 Oct4 SRY sex determining region Y box 2 Sox2 Kruppel like factor 4 KIf4 DTD1 mRNA Transdirect 50xL 15 000 rpm 15 20 9 L DNA DNA DNA 2 DNA Cy52. JOQA 0376 604 8511 1 604 851 1 1 075 823 1351 http www an Shimadzu cO jD 3219 02102 30A IK
3. 1 30 zL 2 DNA 25 10 DNA 25 20 1 DNA DNA Oct4 DNA TGTCGAATGCAAATCACTAGAA 30 v v 9 pL Sox2 DNA CCAAGGCCATTGTAATGCG 10mM Tris HCI pH8 0 KIf4 DNA ATGCAGGAGAAAGAAGGGCGTAGTATCTACTAG 50mM NaCl 1 1 mM DTT 0 05 TritonX100 25 ng hL Salmon sperum DNA DNA 0 eeeel 12 L 5 20 _ 250nM DNA Native PAGE 0 5XTBE 250 V 30 min _ 2SnM Cy5 ONA 30 LL TEL 075 823 1351 WEB https Solutions Shimadzu co jp form biotech contact html E Mail t direct shimadzu biotech jp Transdirect insect cell
4. P N s292 30000 91 Insect Cell Extract X5 4mM Methionine x1 0 5ug uL DTD Vector X1 40 50 L 80C 31 185 Reaction Buffer X1 0 5ug uL Control DNA x1 0 Transdirect insect cell 0 P N S292 30000 92 404 250 50 Transdirect insect ce 50 P N S292 30000 93 9824 000 100 Transdirect insect ce 100 P N S292 30000 94 1 6 1 7 000 pTD1 Vector Control DNA 201 1 1 1
5. C297 0449 SHIMADZUU i BIOTECH Transdirect insect cell DNA EMSA Electrophoresis Mobility Shift Assay DNA Transdirect iPS Oct4 Sox2 KIf4 DNA EMSA i DNA DNA DNA ma a Oct4 Sox2 KIf4 pTD1 mRNA 2 DNA 5 Cy5 DNA Native PAGE 2 2 0 1 0a2 0 RS DNA DNA 3
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