I3-FITC
Contents
1. 10 11 12 HLA D DR 13 13 HLA DR HLA DQ HLA DP HLA DR 12 14 152. 6 7 Fc 10 15 1mg mL 8 9
3. Harvard Medical School Dr S F Schlossman Coulter Immunology T B 3 13 FITC I
4. 4 5 7 DM 13 FITC 2 8 C 24 1 2 8 2 IS3 FITC 2 8 6 0 20 6 70
5. 16 gt PBS PBS 2 8 C o NBS 2 BSA 0 19 0 19 gt 1 500L 100 000 xg 10 gt 1 5L
6. 1 EDTA 6 2 90 3 859 4 Ficol Paque Ficoll Paque 5 5 Ficol Paque
7. HLA D DR MSlgG2a FITC 1 29 29 T T
8. T T 3 9 CV 7 50 HLA DR y 0 95X 2 7 r 0 938 1 0 196 3
9. MSlgG2a FITC 200L 3 4 6 2 8 C 30 7 2 8 C 400 450 x g 5 8 3 4 9 PBS 200L 2 8 C 400 450 x g 5 10 3 4 11 PBS 200L EPICSW Cytomics 1 2 Ficoll Paque 3 FITC Log 1
10. 1 4mL PBS Ficoll Paque 4mL 1 3 2 8 C 400 450 x g 30 4 Ficol Paque 2 8 C PBS 2 8 C 400 450 xg 8 6 PBS 7 2 8 C 400 450 x g 4 8 PBS 9 2 8 C 400 450 x g 3 10 PBS 1 x 10 mm 1 x 10 mL 11 a b 90
11. 6603424 2009 11 2007 2 20300AMY00144000 877429 IS FITC IN ww 1 3 HLA D DR 13 15rg 3 HLA DR DQ DP 34kD w 29kD 13 I
12. 2 5 a 12 1x 10 1x 103 mm 1mL 13 2 8 C PBS 2 8 400 450 x g 4 14 0 05mg mL Propidium lodide 3 1 15 2 8C PBS 2 8C 400450 xg 4 16 15 17 859 b 12 25 000 1 x10 L 25 L 13 Propidium lodide 0 01mg mL 10L 14 30 Acridine orange 0 005mg mL 10L 3
13. 8 0 5 1mL PBS 9 EPICS Cytomics 3 Ficoll Paque 1 1 x 10 200L Ficoll Paque U 96 200pL 2 2 8 C 400 450 x g 5 3 4 5 200L
14. 135 0063 5 7 TOC Wa COULTER 6603424_0911
15. MHC class II HLA D DR HLA D DR B CD4 T HLA D DR B HLA D DR CD3 T T T IS3 I MHC class I HLA D DR HLA DR HLA DQ HLA DP B
16. 15 16 100 85 gt PBS PBS PBS 2 lt 8 NBS 296 BSA 0 196 0 19 PBS PBS 0 196 2 8 C lt gt 1 1 2 100L 3
17. 3x10 mm PBS 20 25 6 2 4 a gt 10 x 10 mm 10 20 x10 2 20 30 x10 3 30 40 x10 5 40 60 x10 6 60 100 x10 10 100 200 x103 20 b lt 3x10 mm 1 25C 500 x g 5 2 3 4 LH 700 5 10x10 mm 1 100 L 2 Ficoll Paque
18. 200L MSlgG2a FITC 200L 4 45 5 a b a 2mL 5 15 b PBS 2 3mL 400450 x g 5 PBS 25 1mL 30 2 250L 6 PBS 2mL 7 400 450 x g 5 8 6
19. 5 L 195L PBS 1 PBS PBS 6603369 1 500mL pH 7 2 0 2 0 196 2 1 2 1 6603152 300 PBS 24mL 1mL 9 259 6 2 1L 8 26g 1 0g EDTA 4Na 2Na 37mg pH 7 2 7 4 1 1
20. 3 lt 10 468 lt 509nm 504 541nm 1 0 5mL 0 2 0 01M 0 15M 0 196 HLA D DR 1 4 HLA D DR 488nm FITC FS 907 SS
21. MHC class I HLA D DR HLA DR HLA DQ HLA DP B 75 I3 B B 16 I3 CFU C T l T I MHC T CD4 13 II MHC I3 9 49 BALB cJ NS 1 AG4 lg IgG2a H zL IS FITC FITC Fluorescein lsothyocyanate FITC
22. 12 3 20 25 474 13 FITC 6603424 100 0 5mL 1 Todd RF Meuer SC Romain PL and Schlossman SF 1984 A monocbnal antibody that blocks class I histocompatibility related immune interactions Human Immunology 10 23 Barclay AN Birkeland ML Brown MH Beyers AD Davis SJ Somoza C and Williams AF eds 1993 The Leukocyte Antigen Facts Book Academic Press London pp 376 377 Ko HS Fu SM Winchester RJ Yu DTY and Kunkel HG 1979 la determinants on stimulated human T lymphocytes Occurrence on mitogen and antigen activated T cells J Exp Med 150 246 Koepke JA and Landay AL 1989 Precision and accuracy of absolute lymphocyte counts Clin Immunol Immunopathol 52 19 27 IN ww 135 0063 5 7 TOC TEL 0120 566 730 FAX 03 5530 2460
23. 7 9 0 5 1mL PBS 10 EPICS Cytomics 2 Ficoll Paque 1 12mm x75mm 2 Ficol Paque 1 x 10 1x10 mm 1mL 3 2 8 C 400 lt 450 x g 4 4 200L MSlgG2a FITC 200L 5 2 8 C 30 6 PBS 1mL 2 8 C 400 450 x g 4 7 5 2
24. 2 30 2 10 15 20 30 3 MslgG2a FITC 6602906 100 0 5mL 4 Propidium lodide Calpiochem 53705 0 01mg mL 0 05mg mL 5 Acridine orange Baker A366 3 0 005mg mL 6 Ficoll Paque Pharmacia 17 0840 03 EDTA 1 1 100L 3 10 x 103 mm 10x10 mm
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