Nikon Digital Camera System User's Manual
Contents
1. Nikon O H 00 a O 7 1 LO i 180 5 DS U L 203 i gt i O N c q t Il O N S Camera Heads DS 2MBWc DS 2 MBW DS eMv CCD DS 2MBWc DS 2MBW DS 2Mv 1 1 8 in high density CCD Total number of pixels 2 11 million effective 1 98 million Recordable pixels 1600 x 1200 pixels 800 x 600 pixels 400 x 300 pixels CCD cooling device Sensitivity Peltier Device Ambient temperature 20 C Equivalent to ISO350 Can be varied between ISO 160 6400 equivalent Equivalent to ISO100 Can be varied between ISO 50 2000 equivalent A D conversion Live display mode Ds L1 12 bit 1600 x 1200 15fps 800 x 560 30fps Center Scan 30fps Display reduced or enlarged to SXGA XGA Live display mode DS U1 1600 x 1200 4fps 800 x 600 15fps 400 x 300 20fps Center Scan 400 x 280 Frame rates are a guide only Indicated rates assume USB data transfer speeds 800 x 600 12fps 400 x 300 20fps Center Scan 400 x 280 30fps Frame rates are a guide only Indicated rates assume USB data transfer speeds Lens mount C mount Exposure time 1 1000 to 600 sec 1 1000 to 60 sec Dimensions 91 0 W x 76 0 D x 41 0 H m2. PC based system that utilizes multi functional imaging software O Thissystemis controlled byaPC via a USB2 0 connection O ACT 2U imaging software enables the display of smooth and easy to see live images as well as image acquisi tion processing analysis and simple measurements via a PC Thanks to the high frame rate 2 megapixel CCD camera the movement of live images is extremely smooth Live images can be viewed on the large LCD monitor built into the control unit DS 5M L 1 ECUPSE 50i For easy image capture without a PC This stand alone control unit which does not require connection to a PC is a real space saver Camera conditions most appropriate to the illumina tion techniques used can be chosen from a menu allowing anybody to easily capture beautiful images D S 5M L1 Module Microscope High definition stand alone type with net work functions Ae O Incorporates a high resolution 5 megapixel CCD with a capture size of y z l 2560x1920 pixels minute details of images can be captured beautifully The camera control unit is equipped with a built in large 6 3 type XGA TFT gt i LCD monitor for live image viewing EO md O Ethernet based network functionality is built in Images can be saved on any given server as well as on the CF card ismboe I uol _ DS 5M U 1 Module Microscope High definition PC based system equipped with multi functional imaging software DS 2MBW L 1 ECLIPSE TS 100F A
3. 3D Tiling View EO ON i i i Show Filters Gr 5 Sobel Filter E Printing oh Piro cela MI pele Ol ORNS NN rn Boy ee ose Mi Recording Mode s laec FIC W Image Analysis 1 Print Main Menu R Displays analytical settings for control and a nia dr Cancel 1 Average Recording 7 EOW Pass Fiter 1 Focus Analysis 2 Print Preview Mode EG j Gass i a focus Mero 2 Time Lapse Recording l TRE 2 Profile Analysis 3 Layout Edit Mode o E ode a 7 Show Merge Group i i i 8 Median Filter l 2 Microscope Control 3 Variable Time Lapse Recording 3 Histogram Analysis T i 4 Z Stack Recording 4 Area ROI Analysis Sv PEEL PURE puntas SaL Settings box which allows detailed ar yS 4 Fixed Scale Bar Setup customizing of the screen layout in 5 Line ROI Analysis addition to the standard and advanced 7 a a a O o i layout Sy stem Line up For industrial applications For biological applications DS 2MBVW c U 1 ecurse TE2000 Offers image display and capture for low light florescence l DS 2Mv L1 smz1c000 Stand alone type that offers high frame rate t for real time observation 0 Maximum frame rate of 30 fps enables the system to be optimized for live Combining a high sensitivity camera equipped with a cooling mechanism pr observation that eliminates thermal noise with software that boasts a wealth of analyti ee gt O Stand alone type featuring an XGA 6 3 type TFT monitor Observation on a cal fun
4. reasonably priced monochrome configuration O Incorporatesahigh resolution 5 megapixel CCD with a capture size of 2560x1920 pixels true to life images can be captured with ease O Controlled from a PC via a USB2 0 connection O ACT 2U imaging software enables smooth and easy display of images O A launcher function is available This allows saved images to be easily transferred to other data application software for processing Easily takes high contrast monochrome images in observations such as phase contrast and DIC Using a high frame rate camera live motion can be quickly and easily confirmed Exapmle of image created using image composition software PC monitor RGB output 100Base TX Diagram Direct print printer USB mass storage class Windows 2000 XP Ethernet USB HUB Re USB keyboard USB mouse Exclusive remote E na controller a Flash CF vann AC power adapter card d Camera Control Unit ta Mikun Nikon a Niron lt lt gt A f DS ee DS emMv Ul lt n DS eMBNC DS UL NISSEN Windows 2000 XP Nikon k CED moe Exclusive camera cable 3m re USB HS Microscope Camera Control Unit DS U 1 fa Speci fi DS 2MBWc DS 2MBW DS 2Mv DS 5Mc DS 5M gi A Y J gt 76 E mm DS UL
5. speeds which enables camera control and live image display at high speed on a monitor ae GE MA variety of screen layouts N 5 WScale display WDistance H point to line The configuration offers a standard screen layout and advanced layouts with a high PS S measurement distance degree of image analysis functionality Each layout can be customized according to need R Standard Layout n De Aomin Ten unas vie Wai E for straightforward operations G SESSOS PAD 9 Y Lom J E Area E Screen patterns E Tool bar a Pod a al The area inside an enclosure outlined by Grid or concentric circle patterns can be Frequently used menu items are displayed as PA the mouse is automatically calculated displayed WXY scale display MW Circular measurement W Angle Me displays diameter circum intersection buttons es ference center of gravity El Live image screen E Key functions El Captured image screen Display of the captured image Superimposition using the Merge function Interactive control of the microscope and camera 2 ect It is easy to produce merged images of multistained fluorescence sam In combination with the ECLIPSE 90i motorized microscope it is possible to a Properties dialog box e AA ples using monochrome photography When you acquire images after control the 90i from the ACT 2U menu and to automatically detect and record For display alteration of the camera s current wne RES initially se
6. CCD that offers a high resolution image size of 2560x1920 pixels It is ideal for acquiring detailed microscopic images under a variety of illumination techniques including bright field phase contrast and DIC DS 5Mc For extremely clear color fluorescence images With its built in Peltier cooling mechanism the tem perature of the 5 0 megapixel color CCD can be main tained at 20 C below room temperature Thermal noise is thus greatly reduced during long time expo sure Stand alone camera control unit DS L E A stand alone control unit incorporating a large high definition monitor offering easy operation and useful functionality E A wide variety of tool functions O Measurement tools Other useful tools r ser N mi oe oe om 3 5 E CARA pes eN a pet ihe E i ug AT 7 e he Stand alone type Does not require a PC or an external monitor W Excellent output options In addition to saving to a Compact Flash card the unit offers these output options MW Two screen split display A frozen image can be displayed alongside the live image for easy 6 3type LCD monitor XGA 1024x768 screen resolution which allows simple fast and accurate r k y TE Sr Ga comparison focusing without the need to enlarge the Distance measurement MW Vertical horizontal measurement p image Analog RGB output Easily measures the distance between any two Easily meas
7. Digital Camera Digital Sight Series Digital Camera System for Microscopy DIGITAL SIGHT Recommended camera heads for general observation These camera heads can be deployed in a broad range of illumination techniques including bright field darkfield phase contrast and Nomarski DIC differential interference contrast Recommended camera heads for fluorescence and ultra low light observations These cameras are ideal for darkfield and flu orescence samples Incorporating a Peltier cooling mechanism cooled types are able to greatly reduce the thermal noise that can be generated from long time exposures DS 2Mv Smooth and comfortable live images Incorporates a 2 megapixel colorCCD that can smoothly display SXGA live motion images at 15 fps 1 max 30 fps A well balanced camera head that enables display of live images and capture of high definition images 1 Using the DS L1 with output to an external monitor DS 2MBW DS 2MBWc DS eMBbNC DS 2MBW High sensitivity imaging The 2MBWc incorporates a Peltier cooling mechanism that minimizes thermal noise Its 2 megapizel mono chrome CCD boasts a sensitivity rating five times greater than that of previous models resulting in a shortened total exposure time and reduced fading and allowing the realization of high frame rate A non cooled model DS 2MBW is also available Captures true to life images DS 5M incorporates a 5 megapixel color
8. Instruments Company E UKA ENVIRONMENTAL MANAGEMENT Accredited by the Dutch Council for Accreditation ISO 14001 Certified NIKON INSTECH CO LTD NIKON INSTRUMENTS INC 1300 Walt Whitman Road Melville N Y 11747 3064 U S A Phone 1 631 547 8500 1 800 52 NIKON within the U S A only fax 1 631 547 0306 www nikonusa com NIKON CANADA INC CANADA phone 1 905 625 9910 fax 1 905 625 0103
9. ctions Maid This system is most appropriate for high level research that demands the capture of weak fluorescence images clearly with a high signal to noise ratio UuoOInPAI3SQO larger screen is possible with an analog RGB output function mm amp Images can be saved to the CF card simply by clicking the capture button DS 5Mc U 1 ecuPSE 90i Offering the potential for integrated control of the camera and microscope gt ap DS 2Mv L 1 1mm 40 a Stand alone type with convenient built in n measuring functions O Maximum frame rate of 30 fps enables the system to be optimized for live observation O Simple measurement functions are built into the camera control unit DS L1 including distance angle of intersection circular measurements area etc Measurement results can be saved together with the image Images can be saved to the CF card simply by clicking the capture button A single cable connection via USB interface is possible from the control unit to a PC and the Eclipse 90i microscope Since the 90i can be controlled from the ACT 2U imaging software the operation of the microscope can be linked with image recording Auto focus image capture during bright field microscopy is possible utilizing the image contrast OD data detected by the camera DS 2Mv L1 eCuPSE 80i Delivering both smooth live motion images and beautiful captured images MENE ESE DS 2Mv U 1 Module Microscope
10. ge 2 0 Step 1 3 Digital zoom Interval shooting 5 to 2400 5 sec 12 hr intervals Up to 16x 8 steps 10 sec 6 hr intervals Exposure metering Average metering Peak hold metering Exposure metering range 3 selectable sizes White balance Set method Color balance adjustable Image adjustments Gamma correction shading adjustment black level adjustment hue wheel variation color saturation adjustment Storage format BMP TIFF JPEG JPEG2000 AVI BMP JPEG 4 step compression Interface USB device port computer control connector USB device port Mass Storage Class support USB host port microscope connector USB host port USB mouse USB keyboard connection Power supply AC100 240V 50 60Hz Power consumption 43VA 138VA Dimensions 180 5 W x 144 5 D x 68 4 H mm 203 W x 77 D x 204 H mm Weight approx 10008 Control unit approx 1300g AC adapter approx 350g Operating environment 0 40 C 85 RH max without condensation System composition Power cord AC adapter Power cord CompactFlash card 128MB Mouse Networking Ethernet 10 100Base TX DHCP compatible HTTP TELNET or FTP server FTP client LCD monitor 6 3 in TFT color LCD XGA 1024 x 768 60Hz External monitor output Analog RGB SXGA 1280x1024 60Hz XGA 1024x768 60Hz Storage media CompactFlash card Type 1 Type II Direct pri
11. m Weight approx 290g approx 260g System composition Camera Cable 3m Optional accessories For wide field of view observations 0 55x Relay lens C mount Camera Heads DS SMc DS 5M CCD DS 5Mc Cooled CCD Camera DS 5M Standard CCD Camera 2 3 in high density CCD Total number of pixels 5 24 million effective 5 07 million Recordable pixels 2560 x 1920 pixels 1280 x 960 pixels 640 x 480 pixels CCD cooling device Peltier Device Ambient temperature 20 C Sensitivity A D conversion Equivalent to ISO64 Can be varied between ISO 32 1250 equivalent 12 bit Live display mode Ds L1 2560 x 1920 3 8fps 1280 x 960 7 5fps Center Scan 15fps Display reduced to SXGA XGA with DS LI Live display mode Ds uU1 1280 x 960 2fps 640 x 480 7 5fps Center Scan 640 x 480 15fps Frame rates are a guide only Indicated rates assume USB data transfer speeds Lens mount C mount Exposure time Dimensions 1 1000 to 600 sec 91 0 W x 76 0 D x 41 0 H mm 1 1000 to 60 sec Weight System composition approx 290g Camera Cable 3m approx 260g Optional accessories For wide field of view observations 0 7x Relay lens C mount Camera Control Units DS UJTL DS U TL Exposure control DS U1 DS L1 Program AE Shutter priority AE Focus AE Manual with AE lock function Exposure correction 13 steps Correction ran
12. nting Direct printing possible without a computer using special printer PictBridge compatible Optional accessories Exclusive remote controller Direct Print license key Direct printer CP900DW Mitsubishi ACT 2U Imaging Software System Requirements Computer type MS DOS PC supporting USB2 0 CPU Pentium 4 1 7GHz or faster Pentium 4 2 4GHz or faster recommended RAM 1GB or more USB2 0 2 ports Hard disk 100MB to install 300MB or more free space to run on launch disk Operating system Windows 2000 Professional SP4 or later English or Japanese Windows XP Professional English or Japanese pre installed versions only Graphics Others 1280x1024 pixels or more 16 bit color or more 24 bit color recommended DirectX 9 0b support CD ROM drive to install Microsoft USB2 0 driver The above system requirements list does not constitute a guarantee that all computers and systems meeting these criteria will be able to run the software Company and product names included in this brochure are the registered trademarks of the respective companies Monitor images are simulated Specifications and equipment are subject to change without any notice or obligation on the part of the manufacturer May 2005 2005 NIKON CORPORATION TO ENSURE CORRECT USAGE READ THE CORRESPONDING MANUALS CAREFULLY BEFORE USING THE EQUIPMENT NIKON CORPORATION Fuji Bldg 2 3 Marunouchi 3 chome Chiyoda k
13. s provided By using an FTP client and HTTP FTP Telnet server functionality it is possible to transmit images to a network or remotely control the camera from a network For wafer IC chip images For fluorescence darkfield obser vations A separate license is needed in order to use the Direct Print function Direct Print function option A 4 For DIC phase contrast observations For printed cir cuit board images Ares le Th fee Real 10 mode PictBridge el c2 03 offer DL he pr The Real 10 mode is selectable with a dedicated Mitsubishi CP900DW It is possible to print directly without a computer using a CMP WB JAH L BERE gy Y With this mode it is possible to print with a device magnification of 10x PictBridge compatible printer and USB connection ah for easy confirmation of sample size CAM VIEW REC MEPA For HANG For metal ___ DS 5M DS 5Mc C mount 0 7X TV Adaptor optional is necessary TOOL SETUP observations ceramic materi Mu Gi ma al images DS 2Mv DS 2MBW DS 2MBWc C mount 0 55X TV Adaptor optional is necessary top Gee v ar Interactive control of microscope and camera option A separate license is needed in order to use the interactive control Ada In combination with the ECLIPSE 90i motorized microscope it is possible to control the 90i from Prp MAS the DS LI menu and to automatically detect and record the microscope sta
14. tting the number of layers and display colors for each the microscope status data with the image including the objective magnifica settings pe c reagent colored images will be automatically displayed By changing tion and fluorescence filter in use Automatic detection of status is also possi El Image thumbnails a al the wavelength for each acquisition this function easily allows a super ble when combined with the ECLIPSE 80i microscope configured with a digi or r area 2 imposed image to be produced tal imaging head 6 Capture window A sjopy Fete Sahnaga Basic image capture control window Scene function for one click optimal photog raphy E The control software of the 90i and digital imaging head can be easily accessed from ACT 2U Advanced Layout Secon ss snsceu a6 S08 adding useful functions aia Gti A E Toolbox ll Tool Bar H Image Processing E Image Display E Process View E File Operation Tool buttons for processing and analysis of 1 Main Tool Bar 1 Direction Resize Control 1 2D Image View E Thumbnail Window E Camera Control images 2 Tool Box 2 Crop Control 2 2D Quadrant View E Experimental Information aa Conneccion Daca Box z Show Analyze Group 3 Annotation Tool Box 3 Duplicate Control 3 3D Orthogonal View Window los te E Process view window Show Duplicate Group hev 4 Graphic Annotation Setup 4 Kirsch Filter 4
15. tus data with the 1129314 IETS image including the objective magnification and fluorescence filter in use Automatic detection o mE of status is also possible when combined with the ECLIPSE 80i microscope configured with a dig res lt ii gt ital imaging head Focus Info Example of the microscope control menu PC based camera control unit Imaging Software DS U L ACT 2U Single cable connection to PC via USB2 0 interface Extremely versatile imaging software that is easy to operate and has wealth of functions to suit almost any needs E Scene mode for one click optimal photography This compact control unit can be connected quickly to a Windows based PC 3 Capture View Capture View via its USB2 0 interface without the need for a separate PCI board Nikon a ai Gr SAT ng For wafer IC chip images For brightfield observations By controlling the camera with a PC the user can expand their system not only to facilitate image capture but also results analysis and image processing with Nikon s ACT 2U Multi functional imaging software Exposure Time For metal ceramic For fluorescence material images darkfield observations For printed circuit board images For DIC phase contrast observations High speed image transfer to PC Two USB ports are provided Connects to a PC via USB2 0 interface USB2 0 offers both wide compatibility and high data transfer
16. u Tokyo 100 8331 Japan www nikon com NIKON INSTECH CO LTD Parale Mitsui Bldg 8 Higashida cho Kawasaki ku Kawasaki Kanagawa 210 0005 Japan Phone 81 44 223 2175 Industrial dept 1 81 44 223 2167 Biological dept fax 81 44 223 2182 www nikon instruments jp eng NIKON INSTRUMENTS EUROPE B V Schipholweg 321 1171PL Badhoevedorp NL Phone 31 20 44 96 222 fax 31 20 44 96 298 www nikon instruments com NIKON FRANCE S A S FRANCE phone 33 1 45 16 45 16 fax 33 1 45 16 00 33 NIKON INSTRUMENTS SHANGHAI CO LTD NIKON GMBH CHINA phone 86 21 5836 0050 fax 86 21 5836 0030 GERMANY phone 49 211 9414 0 fax 49 211 9414 322 Beijing office NIKON INSTRUMENTS S p A CHINA phone 86 10 5869 2255 fax 86 10 5869 2277 ITALY phone 39 55 3009601 fax 39 55 300993 NIKON SINGAPORE PTE LTD NIKON AG SINGAPORE phone 65 6559 3618 fax 65 6550 3668 SWITZERLAND phone 41 43 277 2860 fax 41 43 277 2861 NIKON MALAYSIA SDN BHD NIKON UK LTD MALAYSIA phone 60 3 78763887 fax 60 3 78763387 UNITED KINGDOM phone 44 20 8541 4440 fax 44 20 8541 4584 NIKON INSTRUMENTS KOREA CO LTD KOREA phone 82 2 2186 8410 fax 82 2 555 4415 Printed in Japan 0506 10 Am M Code No 2CE MRGH 1 Certified ISO 14001 by BAG Y NATIONAL mH ACCREDITATION OF CERTIFICATION ISO 14001 BODES ISO 14001 Certified NIKON CORPORATION Yokohama Plant Pu ISO 9001 ISO 9001 Certified NIKON CORPORATION
17. ures the distance between two horizon W Text input line and figure drawing points specified by the user tal and two vertical lines E Thumbnail image display E Superimpose function E Shading function E Focus indicator E Histogram E Digital zoom E Log text saving fl Interval exposure On screen menus Intuitive on screen menu based opera tion allows for simple set up and capture Direct printing using PictBridge USB Mass Storage Class Network connectivity Screen patterns Count marking Cross line and concentric circle patterns can be dis Up to 99 serial numbers per color can be marked to played provide a convenient way to identify points of interest on screen They can be easily saved and printed with the image E Scene mode for one click optimal photography Using Nikon s experience with digital imaging optimal pre programmed imaging modes have been made available as menus including preset camera conditions according to the sample types and illu MScale display WXY scale display Point to line MCircular measurement M Angle mination techniques used Optimal images can be captured with a simple click Users can also cus distance displays diameter circumference intersection tomize settings and save up to seven of these modes for quick retrieval ga BIOLOGICAL E Expanded functionality provided by external connectivity Network functionalit A 10 100Base TX compatible Ethernet port i
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