New Brunswick Galaxy CO2 Incubator 14S Operations
Contents
1. If at any time you wish to change the CO level without adjusting the Temperature setpoint press the Programming 9 key twice to work in the CO display NOTE CO gas will NOT be injected until the incubator reaches its temperature setpoint Allow the incubator to stabilize at the selected setpoints for at least 2 hours before continuing CO14S 0050 Galaxy 14 S CO Incubator User s Guide 21 3 5 Referencing CO with AutoZero The purpose of the AutoZero system is to reference the CO sensor to an atmospheric CO level of 0 05 Over a period of time the sensor baseline may drift causing an inaccuracy in the CO level at the programmed value We recommend that you AutoZero the CO system Prior to using the incubator for the first time Once a month when your incubator is operating to ensure that the CO level is as accurate as possible After the incubator has been in storage or transit for a while To perform the AutoZero 1 If you have not already done so set the CO level see Section 3 4 There should be an audible click when the programmed level is entered this is the CO valve opening 2 Press the and enter keys simultaneously NOTE There is a magnetic switch in the outer door and a magnet mounted above the door to switch off the CO valve when the outer door is opened 3 The outer door and inner door if present must be opened and closed according to the displayed in2. 2 3 1 Oxygen sensor holder on rear wall of incubator 3 Hydrophobic filter disc 2 Hydrophobic filter holder screws into sensor holder 4 Hydrophobic filter cap To replace the hydrophobic filter disc membrane with reference to Figure 11 above 1 Carefully unscrew the hydrophobic filter holder from the oxygen sensor holder on the rear wall of the chamber 2 With a fingertip or a 10 11mm inch rod from the rear of the filter holder gently push the filter membrane disc and the filter cap out of the holder 3 Clean and dry the filter holder and cap 4 Wearing gloves to avoid contaminating the filter disc gently place the new filter membrane disc into the filter holder recess The filter disc works in both directions so there is no right or wrong side 5 Make sure the O rings between the cap amp filter holder and between the filter holder amp oxygen sensor holder are undamaged and securely in place Gently press the filter cap back in 7 Carefully screw the hydrophobic filter holder back into the oxygen sensor holder D 7 2 9 Troubleshooting the Oxygen Sensor If the oxygen sensor fails suddenly it is very likely that the sensor inlet membrane has become blocked by condensation To dry the membrane New Brunswick Scientific User s Guide 42 1 Remove the hydrophobic filter holder assembly by unscrewing it counter clockwise 2 Program the incubator for a temperatur
3. m rn HE The the other possible outcomes are presented in detail in Sections 7 2 5 Replace Sensor Soon and 7 2 6 Replace Sensor Now See also Section 7 2 7 for instructions on removing and replacing the sensor CO14S 0050 Galaxy 14 S CO Incubator User s Guide 39 7 2 5 Replace Sensor Soon If the referencing procedure was successful but the sensor is nearing the end of its working life the following scrolling message will be displayed nrt rEt Sensor ner Endof 2 FE bil m rh E In this case the sensor will need to be replaced soon See Section 7 2 7 for instructions on removing and replacing the sensor 7 2 6 Replace Sensor Now If the referencing procedure failed oxygen control will automatically be disabled until a new sensor is installed and correctly referenced to atmosphere The following scrolling message will be displayed BOREF FHanEH Slasoe Ge deas FE oc conire aff If the sensor fails the referencing procedure first repeat the procedure to confirm the result Then see Section 7 2 7 for instructions on removing and replacing the sensor 7 2 7 Removing amp Replacing O Sensor To remove and replace the oxygen sensor you will need the sensor removal tool provided see Figure 10 on the following page Pull the rear access cover see Figure 9 below off the rear outside wall of the incubator to gain access to the oxygen sensor Figure 9 Oxygen Sensor Rear Access Cover New Brunswic
4. 1 Inspection of Boxes After you have received your order from New Brunswick Scientific inspect the box es carefully for any damage that may have occurred during shipping Report any damage to the carrier and to your local NBS Sales Order Department or distributor immediately 2 2 Unpacking A l CRUSH WARNING At least two people are required to safely lift your 14 S by hand Disassemble the shipping crate and remove the cardboard fitted foam and protective packing Save the packing materials for possible future use and be sure to save this User s Guide for instruction and reference Locate and remove the parts stored inside the incubator and the bag outside the incubator that contains tubing and the power cord If any part of your order was damaged during shipping is missing or fails to operate please fill out Customer Satisfaction Form 6300 packed in the envelope with your warranty card and return it by fax or mail You can also call New Brunswick Scientific s or your distributor s service department Using your NBS packing list verify that you have received the correct materials and that nothing is missing Table 1 on the following page outlines the accessory items that are supplied with your new incubator New Brunswick Scientific User s Guide 12 Table 1 Accessories Provided Quantity Item Notes 2 Non tip Shelves Packed separately 2
5. 8 minutes to 696 7 3 BMS Relay Contact Alarm Option The BMS Building Management System Relay Contact Alarm allows a signal from a central alarm system to be switched ON or OFF to indicate an alarm condition at the incubator The following alarms would activate the system over temperature under temperature system failure CO2 high and CO low CO14S 0050 Galaxy 14 S CO Incubator User s Guide 43 As an integral option the alarm can be programmed to indicate when the power fails perhaps due to an electrical fault or is switched off If the power failure warning is active the relay contacts will be reversed pin 4 which is normally open becomes normally closed and pin 6 which is normally closed becomes normally open The alarm will also respond to other types of alarm depending on the options installed on the incubator The system is connected low at the middle of the equipment tray as you face the rear panel via a standard 6 pin DIN socket see Figure 12 The matching plug is provided when the option is installed in the accessories bag Figure 12 BMS Relay Contact Alarm Socket Table 6 BMS Relay Contact Alarm Socket Pin Designation Pin Designation 1 12VDC unregulated To power external equipment such as a remote buzzer 2 OV or light 100mA maximum current available 3 5VDC Via 10KQ pull up resistor for a logic signal to directly control an auxiliary control system 4 Normally
6. Guide 18 3 OPERATION 3 1 Control Panel The control panel consists of two individual three digit LED displays and four function keys see Figure 6 below Figure 6 Control Panel Temp C 96CO oo 0 1 Temperature display 2 CO display 3 Function keys Table 3 Function Keys Key Symbol Key Name Function Press this key to enter Programming mode and to e Programming set values in either display Press this key simultaneously with the Enter key to perform AutoZero In Programming mode use this key to scroll down v Down through numbered values in the display Press this key simultaneously with the Enter key to work in the Alarm system A U In Programming mode use this key to scroll up P through numbered values in the display Press this key to save a new setpoint Press this key simultaneously with the Programming key to perform AutoZero Press this key simultaneously with the Down key to work in the Alarm system enter Enter NOTE If you accidentally press the Up amp Down keys simultaneously you will engage Engineering Mode immediately press the Programming key 9 to exit CO14S 0050 Galaxy 14 S CO Incubator User s Guide 19 3 2 Preparing for Operation 1 Remove the black protective cover from the CO sensor if not already removed and store it for use later when you clean the incubator There is a storage hol
7. NBS factory without prior authorization from the New Brunswick Scientific New Brunswick Scientific User s Guide CO14S 0050 Galaxy 14 S CO Incubator User s Guide TABLE OF CONTENTS 1 INTRODUCTION o cc vn on un i6 uo ao oo e a uo Ge uo no uo e ug an een o o o e o n nn o o o uo e n n n e o n e c ua uu ut nee 9 2 UNPACKING amp INSTALLATION 0000000000000000000000000 nhe he hehehe hee e eee e eee ea aea e au 11 2 1 INSPECTION OF BOXES eese ne nenneheh eren eene i se etes i esee essen esiste ee eee 11 22 UNPACKING 2 teeisisicecebes terea e e dU GNI EU EET D UT HIIS are debes gate ee 11 2 3 NG v i D H N i otis ee E te beet cuve ot eee E O 12 24 LOCATION zu ea venei esteso E reboot doe Pot rest aloe tere 12 2 5 INSTALLING THE PRET ois eee e ede eres EN e tdeo ee teet ed ve eee ee bee e lan 13 2 6 SETTING BEREIN 13 OPERATION cecciccscsccccdicicsscccctesccccscccssocssseccdacsossocsecooscdecedoodeccdeonevosssoncccecsasscsdccoodeccdseases 18 3 1 EONTROEPANEE ur A EES EEE EE 18 3 2 PREPARING FOR OPERATION ccccccceccccccccssssssssccccccceseessescceccsssseussesceesssssuuesssescesess 19 3 3 USING THE HUMIDITY TRAY ccccccccccccccsssssscscccccccessesscsccecsssssseesecsccecssseuauseaescesess 19 3 4 SETTING TEMPERATURE amp CO cecscasancsessseseenennnnnnsnsnsnnnnnnnnnnnnnnsnnnnennnnnnnnnsnnenennnnnn 20 3 5 REFERENCING CO WITH AUTOZERO cccsssssccccccccsssssssccccsessssusss
8. Safety requirement for electrical equipment for measurement control and laboratory use Classification Class 1 Installation Category 11 Pollution Degree 2 EN 50081 1 1992 EN 50082 1 1992 Electromagnetic Compatibility Generic Emission Immunity Standard Following the provision of Directive 73 23 EC Low Voltage Directive 89 336 Electromagnetic Compatibility SV Mike King L Managing Director New Brunswick UK Operations 9 April 2009 RS Biotech Laboratory Equipment Ltd 1 Drummond Crescent RS Biotech is part of QF49 Irvine Ayrshire KA11 SAN SCOTLAND UK New Brunswick Scientilic an Eppendorf company CO14S 0050 Galaxy 14 S CO Incubator User s Guide 35 7 OPTIONS amp ACCESSORIES 7 1 Options Some combinations of options may not be possible Others may incur extra cost Please inquire before ordering Option NBS Part Number Oxygen Control 1 19 CO14S 120 0200 120V CO14S 230 0200 230V BMS Alarm Relay Contact P0628 6300 7 2 Os Control 1 19 Option This oxygen control option is designed to cover the 1 19 range by adding nitrogen to bring the level below ambient 7 2 1 Setting Up the N Tank Before you set up your oxygen control be sure you have the proper equipment for your nitrogen supply 2 cylinders of nitrogen regulation size W two stage pressure regulator 1 Inline pressure regulator 6mm PVC tubing tubing clips Set up the nitrogen tan
9. Wire Shelf Racks one for each side Packed separately 1 Humidity Tray Packed separately 8 Silicone Rubber Extruded Rack Guards Installed 1 White porous CO Sensor Cover Installed 1 Black Sensor Cover Installed 6 Silicone Rubber Suction Pads Packed in accessories bag 1 Power Cord Packed in accessories bag metes PVC Tubing 1 4 inch or 6mm bore Packed in accessories bag 9 8 feet with an inline CO2 HEPA filter connected ready for use 2 Hose Clips Packed in accessories bag 4 Adjustable Feet with locking nuts Packed in accessories bag 4 Anti slip Pads for adjustable feet Packed in accessories bag 1 User Manual Supplied WARNING Anytime you touch or handle the white CO sensor cover be sure to wear gloves and do not later touch those gloves to your face Discard or wash the gloves 2 3 Utilities In order to use the incubator you will need Table 2 Utilities Utility Requirement Electricity 110 120V 50 60 Hz grounded electrical supply with min capacity of 6 amps OR 220 240V 50 60 Hz grounded electrical supply with min capacity of 3 amps CO Gas Cylinder with 100 CO vapor withdrawal together with a two stage regulator for pressure control to 5 psi 0 35 BAR up to 15psi 1 04BAR when using O2 control 2 4 Location The incubator is designed to operate at a chamber temperature ranging from 5 0 C above ambient to 50 C Be sure to avoid placing the incubator in a location that may affect its per
10. closed 5 Common 6 Normally open To access the relay contacts Contact limits are 3 Amps 24V DC and 3 Amps 34 VAC Cable length should not exceed 3 meters 9 8 ft to comply with EMC requirements To turn the BMS relay contact alarm option ON Press the enter and V keys simultaneously to enter the alarm menu Press the A key until 7Zz 7 7is shown in the display Press the enter key to select this option The default setting is OFF which reads 77 in the display Press the A key to change the display to 7 5 ON To deactivate the option you can press the W key to return the display to 74 OFF Press the enter key to accept the selection 6 To return to the main temperature and CO2 display screen press the 6 key twice a mi New Brunswick Scientific User s Guide 7 4 Available Accessories Table 7 Available Accessories Accessory NBS Part Number CO Bottle Automatic Change Over Controller P0628 5000 CO Two Stage Regulator P0628 5010 CO Supply Line Filters HEPA P0628 5020 In Line Pressure Regulator P0628 5030 CO Gas Analyzer Kit P0628 5040 10 Spare CO Gas Analyzer Tubes P0628 5050 Electronic CO Gas Analyzer P0628 6150 Stacking Bracket For 2 incubatorss prevents accidental P0628 6230 tipping Extra Shelf Non perforated P0628 6180 Extra Humidity Tray 0 3 liter P0628 6200 Multi Position Wire Shelf Racks 4 position P0628 6170 In
11. immediately CO14S 0050 Galaxy 14 S CO Incubator User s Guide 27 3 8 4 Review of Alarm Messages For easy reference Table 5 provides an overview of all alarm messages that you may encounter across both displays Table 5 Alarm Messages Message Meaning Ref Section doo r A door open 3 7 3 LF AL temperature sensor failure 3 8 1 SAH sensor alarm after sensor recovers 3 8 1 Lk rv iF over temperature cut out has cut power to 3 8 2 heating elements and CO valve LAL over temperature defect has occurred and 3 8 2 system is under emergency control LOF AULD CEr ll FHL CO sensor is defective and needs to be 3 8 3 replaced New Brunswick Scientific User s Guide 28 4 ROUTINE MAINTENANCE 4 1 General Notes To ensure that chamber conditions remain as stable as possible be sure to minimize the length of time that the inner door if fitted is open The magnetic door catches are specifically designed to make door opening and closing as easy as possible When you open the door wipe off any small drops of condensate that may have formed on the inner seal This will avoid a build up of condensation If you are using the humidity tray for humidification be sure to follow the indications provided in Section 3 3 NOTE There is no need to remove the top panel for normal maintenance or servicing so if you have two incubators stacked the upper incubator does not have to be moved when
12. state infrared CO sensor operating independent of GO Control humidity Semi automatic CO 2E GRE facility Range 0 2 20 Control 0 1 Stability 0 2 Uniformity 0 1 Recovery Rate Better than 0 7 minute Gas Connections 6mm 1 4 inch tubing Required Gas Pressure 0 35 bar 5 psi Relative Humidity Removable stainless steel humidity tray Reservoir Capacity 0 3 liters Fill to 0 2L Humidity Control 90 to 95 condensation may occur at the High setting Shelves Stainless steel non perforated standard Capacity 405 cm per shelf Number of Shelves 2 standard with multiple position option up to 4 shelves Two level alarm system giving programmable audio visual warnings with options for remote communication Level 1 signals system failures level 2 is programmable and monitors chamber conditions Alarm Systems Dimensions 294 x 233 x 208mm Chamber HxWxD 11 6 x 9 2 x 8 2 inches 14 liters Chamber Volume 0 5 cu ft 454 x 313 x 285mm External HxWxD 17 9 x 12 3 x 11 2 inches 580 x 440 x 400mm pallet included 22 8 x 17 3 x 15 7 inches pallet included continued External Crated HxWxD 1 RT Resistance Temperature curve 2 If the incubator is to be run close to ambient temperature it may be necessary to make some adjustments to the control parameters to avoid temperature overshoot Contact your NBS representative for details CO14S 0050 Galaxy 14 S CO Incuba
13. which if not followed correctly could result in serious personal injury This particular Warning message represents a potential WARNING electrical hazard A This particular Warning message whether found in the manual or on the incubator means HOT SURFACE and therefore represents a potential danger to touch Crush Warning messages alert you to specific procedures or practices regarding heavy objects which CRUSH not followed correctly could result in serious personal WARNING injury This symbol on the incubator is a reminder that it is of essential importance to read the user manual CO14S 0050 Galaxy 14 S CO Incubator User s Guide gt WARRANTY lt Every Instrument manufactured by New Brunswick Scientific NBS is warranted to be free from defects in material and workmanship This apparatus with the exception of glassware lamps and electrodes where supplied is warranted for 2 years against faulty components amp assembly and our obligation under this warranty is limited to repairing or replacing the instrument or part thereof which shall within 2 years following date of shipment prove to be defective after our examination Incubator accessories are warranted for 1 year This warranty does not extend to any NBS products which have been subjected to misuse neglect accident or improper installation or application nor shall it extend to products which have been repaired or altered outside the
14. you are servicing the lower incubator 4 2 Daily Checks 1 Check that the temperature and CO levels are reading within specification 2 Check the reserve pressure in the CO cylinder normally 725 psi or 50 bar when full The design of the incubator ensures very low consumption of CO2 during normal working conditions a typical large cylinder should last approximately 12 months If there is a significant drop in the cylinder pressure from 725 psi or 50 bar it means that the cylinder is almost empty and should be replaced Ensuring that there are no leaks at any of the connections will ensure a greater lifetime to the CO supply and will help avoid accidentally running out of CO 3 Any spills in the chamber should be cleaned immediately to protect the stainless steel surfaces 4 3 Monthly Checks 1 Refill the humidity tray to a maximum volume of 0 2 liter The use of warm distilled water 37 0 C will ensure a rapid return to optimum chamber conditions After refilling check that the humidity tray is situated between the shelf rack supports CO14S 0050 Galaxy 14 S CO Incubator User s Guide 29 2 If required you can take a sample of the gas inside the chamber using the CO2 sample port and check it using a CO gas analyzer The CO sample port is located on the lefthand side of the rear panel see Figure 5 If you conduct a sampling please take the following precautions Turn off the CO gas by reprogram
15. 21 Running the 21 AutoZero Failed Message 26 B BMS Relay Contact Alarm 42 C CAUTION Symbol for 4 Chamber Alarm System Functions of 24 Chamber Alarms Flow Chart 25 CO Control System Alarm 26 CO Display 18 CO High Alarm 23 9 INDEX CO Low Alarm 23 CO Setpoint Range 20 Condensation 28 Control Panel 18 Copyright Notice 4 D Daily Checks 28 Declaration of Conformity 33 Direct Heating System 9 Disclaimer Notice 4 Disinfecting 30 Displays 18 Door Open Alarm 23 Down Key 18 Drawings List of 45 E Enter Key 18 F Factory Set Setpoints 19 Filter Disc Replacing the 40 Function Keys 18 H HEPA Filter 12 Humidity Tray 12 Hydrophobic Filter 40 Replacing the 40 I Infrared CO Sensor 9 Inner Door 10 International Offices List of 2 Introduction 9 CO14S 0050 Galaxy 14 S CO Incubator User s Guide 47 Keypad 18 L Leveling the Incubator 17 Location 12 M Maintenance 28 Manual Conventions 4 Monthly Checks 28 N Nitrogen Supply Connecting the 36 Nitrogen Tank Setting up the 35 NOTE Symbol for 4 O Options Factory Installed 35 Retrofittable in Field 35 Over temperature Safety 9 Over temperature Cut Out Alarm 26 Oxygen Control 1 19 35 Enabling 36 Operating Guidelines for 37 Setting Up 36 Specifications 42 Oxygen Sensor Replacing the 39 Oxygen Sensor Referencing to Atmosphere 36 37 38 Removing the 39 Oxygen S
16. IMEOUT 4 COMPLETED ARM ALARMS Y IS TEMPERATURE OR CO2 LEVEL MORE THAN 0 5 OUTSIDE PROGRAMMED POINTS VISIBLE AND AUDIBLE gt gt ALARM MESSAGE ONM 4 4 SCREEN PRESSING ANY KEY Go HAVE CONDITIONS VISUAL AND AUDIBLE ee RECOVERED TO ALARMS CANCELLED PRESSING ANY WITHIN 0 1 MESSAGE LEFT ON SCREEN KEY Temperature Sensor System Alarms 3 8 1 There are four temperature sensors two in the door and two in the chamber If any of these sensors should fail the following message will appear L FML Because the incubator can no longer control temperature properly without the failed sensor the heating will switch off and the incubator will cool down to room temperature User s Guide New Brunswick Scientific 26 If however a sensor fails but subsequently corrects itself the temperature control will restart and an alarm message will remain on the temperature side of the display 5 7 meaning Sensor Alarm This message can be cancelled by pressing any key 3 8 2 Over Temperature Cut out amp Alarm This alarm only occurs if the chamber temperature exceeds the Temperature setpoint by 1 C or if one of the heating elements exceeds a specific activation threshold The activation threshold is set at the factory and cannot be adjusted Following activation the over temperature cut out amp alarm system o
17. New Brunswick u aneppendorf company Guide to Operations Galaxy S Series 14 S CO Incubator MANUAL No CO14S 0050 Revision B February 16 2010 New Brunswick Scientific PO Box 4005 44 Talmadge Rd Edison 08818 4005 USA 1 800 631 5417 1 732 287 1200 bioinfo nbsc com www nbsc com INTERNATIONAL OFFICES BELGIUM New Brunswick Scientific NV SA Stationsstraat 180 4 3110 Rotselaar Belgi Belgique Tel 32 0 16 56 28 31 Fax 32 0 16 57 27 53 E mail sales nbsnv sa be CHINA New Brunswick Scientific A903 Yin Hai Building No 250 Cao Xi Road Shanghai 200235 P R China Tel 86 21 6484 5955 or 5966 Fax 86 21 6484 5933 E mail nbschc gonline sh cn FRANCE Eppendorf France SARL 60 route de Sartrouville 78230 Le Pecq France Tel 33 0 1 30 15 67 40 Fax 33 0 1 30 15 67 45 E mail eppendorf eppendorf fr GERMANY Eppendorf Vertrieb Deutschland GmbH New Brunswick Produkte Peter Henlein Strasse 2 D 50389 Wesseling Berzdorf Deutschland Tel 49 0 2232 418 0 Fax 49 0 2232 418 155 E mail vertrieb eppendorf de THE NETHERLANDS New Brunswick Scientific BV Kerkenbos 1101 6546 BC Nijmegen Nederland Tel 31 0 24 3717 600 Fax 31 0 24 3717 640 E mail sales nbsbv nl UNITED KINGDOM New Brunswick Scientific UK Ltd 17 Alban Park Hatfield Road St Albans Herts AL4 0JJ United Kingdom Tel 44 0 1727 853 855 Fax 44 0 1727 835 666 E mail bioinfo nbsuk co uk C
18. O14S 0050 Galaxy 14 S CO Incubator User s Guide CAUTION This equipment must be operated as described in this manual If operational guidelines are not followed equipment damage and personal injury can occur Please read the entire User s Guide before attempting to use this incubator Do not use this equipment in a hazardous atmosphere or with hazardous materials for which the equipment was not designed New Brunswick Scientific NBS is not responsible for any damage to this equipment that may result from the use of an accessory not manufactured by NBS New Brunswick Scientific User s Guide 4 Copyright Notice New Brunswick Scientific Box 4005 44 Talmadge Road Edison New Jersey 08818 4005 Copyright 2010 New Brunswick Scientific All Rights Reserved Reproduction adaptation or translation without prior written permission from New Brunswick Scientific is prohibited Disclaimer Notice New Brunswick Scientific reserves the right to change information in this document without notice Updates to information in this document reflect our commitment to continuing product development and improvement Manual Conventions 0 NOTE Notes contain essential information that deserves special attention Caution messages appear before procedures which if caution is not observed could result in damage to the CAUTION equipment IN Warning messages alert you to specific procedures or WARNING practices
19. aks 3 Turn on the gas supply with the pressure set to 0 35 bar 5 psi 0 NOTE Be certain to check for leaks in the CO connections to avoid depleting your CO gas supply This can be accomplished using a solution of soapy water applied to each fitting and checking for bubbles If any bubbles are noted readjust the fitting WARNING Slightly increased levels of CO may be found in and around the operating area of the CO incubator Over time this can have adverse effects on those exposed to such an environment Users working in environments with elevated levels of CO should take all appropriate precautions to protect their breathing 4 Remove the black protective cover from sensor store it on the back of the incubator for use when disinfecting Ensure that the white porous sensor cover remains in place CAUTION Failure to remove the black CO sensor cover will cause the incubator to operate incorrectly CO14S 0050 Galaxy 14 S CO Incubator User s Guide 17 Figure 5 Rear Panel CO sensor cover holder CO gas inlet 7 4A fuse CO sensor cover holder HEPA filter o N Power supply O sensor optional Install the power cord 1 Insert the power cord into its receptacle on the bottom left of the incubator s rear panel see Figure 5 above 2 Press the cord firmly into its socket New Brunswick Scientific User s
20. creen You can acknowledge and cancel the alarm by pressing any key When the inner door if fitted is opened the Alarm System is disabled When you close the outer door the preset Alarm Arming Delay starts When the delay time expires the Alarm System is rearmed if the temperature and or CO should fall below or rise above the alarm setpoints the alarm will be activated If chamber conditions recover within the Alarm Arming Delay time the Alarm System will be rearmed but no alarm will be activated If an alarm is not acknowledged but the chamber conditions subsequently recover the audible alarm will be cancelled but the alarm message will remain on the screen to alert the user to the fact that an alarm has occurred You can cancel this alarm message by pressing any key The duration of the audible alarm can be adjusted from inactive to continuous see Section 3 7 4 above CO14S 0050 Galaxy 14 S CO Incubator User s Guide 25 Figure 8 Chamber Alarm System Flow Chart CANCEL ALARM BY ALARMS CANCELLED CHANGE OPEN INNER DOOR SWITCH ON PROGRAMMED VALUES v DISARM ALARMS Y CLOSE INNER DOOR la ARE TEMPERATURES AND CO2 LEVELS WITHIN 0 1 OF SET 4 6 POINT Y MEL 15 MINUTES ALARM DELAY TIMEOUT STARTED YES S v 15 MINUTES ALARM DELAY T
21. der on the rear panel of the incubator see Figure 5 above 2 Ensure that the white porous sensor cover is in place 3 Remove the silica gel dessicant sachet 4 Using the power cord provided connect the incubator to a grounded power supply The display will illuminate immediately NOTE The incubator does not contain a main power switch and will turn on immediately when connected to a power source If the power receptacle has a switch make certain the power receptacle is switched on When the incubator turns on the decimal points on the display for Temperature and CO flash on and off to signify that the Alarm System is not armed 5 Turn on the gas supply if not already on with the pressure set to 5 psi or 0 35 bar for incubators without oxygen control NOTE Be certain to check for leaks in the CO connections to avoid depleting your CO gas supply This can be accomplished by applying a solution of soapy water to each fitting and checking for bubbles If any bubbles are noted readjust the fitting 6 The chamber setpoints are preprogrammed at 37 0 C and 0 096 CO 7 If you are not planning to humidify the incubator leave the humidity tray in place and skip to Section 3 4 below 3 3 Using the Humidity Tray 1 Fill the humidity tray with 0 2 liters of warm 37 0 C distilled water 2 For cell culture work we recommend the addition of one small teaspoonful 0 11 0z 3 2 g of copper sulphate or another recogni
22. e Ferric Chloride or Sulphuric Acid 7 Wipe the internal components of the chamber twice with the alcohol water solution Wipe off excess liquid and leave it to dry completely 8 Reassemble the shelf racks the humidity tray and the shelves before switching the incubator on 9 Be sure to remove the protective cover from the sensor and put it back in the holder for safekeeping 10 When you reinstall it ensure that the humidity tray is centered between the shelf rack supports Refill the humidity tray as explained in Section 3 5 11 Leave the incubator on for at least two hours preferably overnight to allow conditions to stabilize 12 When the incubator has stabilized carry out an AutoZero and reprogram the desired CO level It may be necessary to open the door briefly if after performing an AutoZero the CO level is too high New Brunswick Scientific User s Guide 32 6 SPECIFICATIONS Model 14 S Incubator Specifications Digital programming via microprocessor control in 0 1 C increments Measurement of chamber outer door amp both heating element temperatures via 4 RT matched thermistors sensitivity 0 01 C Out of Limits temperature protection system independent of microprocessor control Temperature Management Range 5 C above ambient temperature to 50 C Control 0 1 C Stability 0 1 C Uniformity lt 0 2 C Solid
23. e of at least 37 C or higher if you normally operate the incubator at a higher temperature 3 Close the door and allow the temperature to recover 4 Reopen the door for 15 seconds to release any build up of humidity 5 Repeat steps 3 and 4 every 30 minutes or so The oxygen level should rather suddenly recover after a few hours 6 Leave the incubator for a few more hours to be sure that the sensor membrane has thoroughly dried out 7 Replace the hydrophobic filter disc following the instructions in Section 7 2 8 Re humidify the incubator 9 After 2 or 3 hours reference the oxygen sensor to atmosphere see Section 7 2 4 When the referencing has been successfully completed the incubator is ready for use ve 7 2 10 Specifications The Oxygen Control option has the following characteristics Sensor Type Self powered diffusion limited electrochemical cell with temperature compensation Zero Signal in Nitrogen lt 50 uV Temperature Compensation 2 of signal variation from 0 40 C Relative Humidity Range 0 99 non condensing Operating Temperature Range 20 C to 50 C Resolution 0 01 Oxygen Expected Operating Life 1 2 years in ambient oxygen Hydrophobic Filter Operating No data available on the filter lifespan but we are Life confident to expect it will last at least 6 months Nitrogen Input Rate 20 l min at 5 psi Typical Oxygen Reduction 3 minutes to 1696 Rates 4 minutes to 1196
24. eceecssssueuaesescecess 21 3 6 COs RECOVERY AAE E E AEE A E E A EA EE ees equ tedu devel ode Peso t Die ev Sue 22 3 7 PROGRAMMING THE ALARM SYSTEM sccsssssccccccccssesssescccccccsssesscececcesssseuuenesceeess 22 3 7 1 Setting High amp Low Temperature Alarms eese enne 22 3 7 2 Setting the CO High amp Low Alarms ueeeeeseseneeensnsnnesnsnennnnnnnennnnnnnensnnnnnsnsnnnnnne 23 3 7 3 Door Open Alarm Asus ee ee 23 3 7 4 PO ED E7111 1 A IR A AA NE E PE AAN AES EAE E A AET 23 3 7 5 Ala m Arming Delay 24 3 8 CHAMBER ALARM SYSTEM ceeeeennannnssesssseeennnnnnsnsnsnssnnnnnnnnsnsnsnsnssnennnnnssssssssnennnnsnssnenn 24 3 8 1 Temperature Sensor System Alarms eese esses eene enne 25 3 8 2 Over Temperature Cut Out amp Alarm eese eene 26 3 8 3 GO Control System Alam d eodd dudes vs e atr dU FU d cda queda v E s 26 3 8 4 Review or Alarm Messages 5 diee ee inea aaa 27 4 ROUTINE MAINTENANCE esssssssssssssssssssssessssssssssssssssssososssssosssssssssososssssosssssssssss 28 4 1 GENERAL NOTES 2 pede E nai iiie 28 4 2 DATEY CHECKS 2 25 05 oa d teet ie er ete ie eite eee let tee eee dodo ertet ee a 28 4 3 MONTHEY CHECKS 4 tet SEEE EE E ee dele et eee 28 5 CLEANING amp DISINFECT ING 0000000000000000000000000000000000000 00000000 sese hehehe sese e ae ae 30 5 1 CLBEANENG netten een Teer IT ae agent 30 5 2 DISINFEEHNG 2a ae engen e ee dcr vr 30 6 SPECIFICATIONS cccccccsc
25. ence the oxygen sensor to atmosphere see Section 7 2 4 once a month to ensure that any long term drift in sensor output will be corrected Be sure to do it at the chamber operating temperature When you are cleaning the chamber be very careful not to wet the oxygen sensor or CO sensor Never use solvents on the sensor membrane rather be sure to cap the hydrophobic filter before you clean In fact it is good practice to replace the filter each time you clean the incubator chamber to avoid the possibility of filter contamination Under normal relative humidity conditions ambient up to 9596 the oxygen sensor s performance should not be affected If however liquid condenses around the sensor gas flow may become restricted giving the sensor a low signal This may occur if there is a large liquid spill inside the chamber or if the incubator is turned off while it is fully humidified Should such condensation appear normal operation can be restored by e removing the humidity tray e drying the chamber completely e and then running the incubator at 37 C for one hour New Brunswick Scientific User s Guide 38 This will dry out the sensor s The humidity tray can then be reinstalled and the incubator can be safely re humidified 7 2 4 Referencing to Atmosphere The oxygen sensor is a self powered electrochemical cell that has a finite life dependent on the ambient oxygen level A typical lifespan is 1 2 years at atmosphe
26. ensor Removal Tool 39 Oxygen Sensor Rear Access Cover 39 Oxygen Sensor Rear Access Cover 40 Oxygen Sensor Removal Tool 40 Oxygen Sensor Troubleshooting the 41 P Power Cord 12 Power Switch Lack of 19 Programming Key 18 PVC Tubing 12 R Recalibration 33 Replace Sensor Now 39 Replace Sensor Soon 39 RS232 Port 10 S Sealing Inner Door 10 Sensor Covers 12 19 31 Sensor Removal Tool 39 Setpoints Factory Set 19 Setting CO 20 Setting Temperature 20 Setting Up Nitrogen Tank 35 Setup 13 Setup Concerns Issues to Avoid 13 Shelf Racks 12 Shelves 12 Specifications 32 Oxygen Control 42 T Table of Contents 7 Tables List of 45 Temperature High Alarm 22 Low Alarm 22 Temperature Display 18 Temperature Sensor System Alarms 25 Temperature Setpoint Range 20 U Up Key 18 New Brunswick Scientific User s Guide 48 Utilities 12 Symbol for 4 Warranty 5 WARNING CO14S 0050 Galaxy 14 S CO Incubator User s Guide
27. et the N tank s outlet pressure gauge to 1 5 bar Set the nitrogen regulator to 0 35 bar 5 psi The nitrogen will be fed into the incubator through a HEPA filter that is already installed in the gas line The gas flow rate is approximately 20 liters minute If the programmed O level is close to the ambient oxygen it may be necessary to reduce the cylinder pressure below 1 bar to stop the oxygen level from undershooting the programmed value 4 After the incubator has been humidified and left overnight to stabilize the oxygen sensor should be referenced to atmosphere see Section 7 2 4 Enable the oxygen control a enter the ZZ zz menu by pressing and holding the A key then pressing the amp key ZZ zz should appear on the display if it does not press the A or V key until it appears now press the enter key b using the A or V key select 777 or JFF to enable or disable oxygen control c press the enter key to store the change d press the key repeatedly until you return to the Main screen Program the required oxygen level a from the Main menu press the key then press the enter key twice the screen should display ZZ 7 Z7 and the 19 0 should be flashing b now use the A or W key to set the desired oxygen level c press the enter key to store the change The oxygen alarm levels are set automatically to 0 5 of the programmed value but you can modify them in the alarm menu as explained in Ste
28. formance such as those listed below CO14S 0050 Galaxy 14 S CO Incubator User s Guide 13 DO NOT place the incubator e Directly under beside or within the air flow of heating or air conditioning ducts or other drafts e Directly beside heat generating equipment such as a heater an autoclave or oven e Near the exhaust of heat or cold generating equipment like a ULT freezer e Near a window exposed to direct unshaded sunlight Remove the incubator from the pallet and place the incubator in the working position on a level surface capable of bearing its weight approximately 12 5kg 27 6lbs for an empty incubator actual operation weight will be heavier and will depend on the options installed and the material stored in the incubator NOTE If you plan to stack two incubators be sure to factor the extra weight second incubator plus options and contents into your choice of location and be sure to use the stacking stand NBS Part P0628 6230 for a safe installation The incubator is designed so that one incubator can be safely stacked on top of another identical incubator Itis not possible to put any other type of incubator or heavy apparatus on top as the top cover is designed to support only the feet of another model 14 S 2 5 Installing the Feet To ensure adequate airflow for correct operation the incubator feet must be installed The feet come in two thread sizes there are two small thread sized
29. front feet and two large thread sized rear feet To insert the adjustable feet 1 Make sure the locking nuts are installed onto each of the four feet provided 2 Putan anti slip pad provided on each foot 3 Beginning with the front pair tilt the incubator toward the back and screw the feet in to the required depth 4 Tilt the incubator forward to install the rear pair of feet in the same manner as above 2 6 Setting Up Install the wire shelf racks and shelves 1 Each wire shelf rack has silicone suction cups and split tubing that hold the rack in place Attach three of the suction feet and four of the split silicone tubing sections to each shelf rack in the positions shown below in Figure 1 Leave a gap of approximately 10mm inch between the bottom of the silicone tubing and the bottom shelf rung This will allow the bottom shelf to be inserted with ease New Brunswick Scientific User s Guide 14 Figure 1 Shelf Rack Set Up left side 1 Leave a 10mm gap here 2 Rear suction cup 2 Place the shelf racks in the incubator chamber making sure that each rack s rear suction cup is pressed firmly against the rear of the chamber and that each piece of silicone tubing is touching the chamber wall As you will discover they will fit correctly only one way see Figure 2 Figure 2 Shelf Rack Installed left side N i 3 Beginning with the bottom shelf install both shelves see Fig
30. h the available choices you will see 444 7 5 z JU SEc bU SEc bU SEc Hr then Zr New Brunswick Scientific User s Guide 24 3 Press the enter key to select the desired value 3 7 5 Alarm Arming Delay This is the length of time that is allowed for the Temperature and CO to recover after you open the incubator and before the Alarm System is armed again This helps prevent unnecessary alarms from occurring To change the delay 1 In alarm menu see step 1 of Section 3 7 1 above press the A key The display will show or z Z across both windows 2 Press the enter key and the A amp W keys to adjust the Alarm Arming time as you scroll through the available choices you will see 7 5 Ar CU UU LUC then LFF 3 Press the enter key to select the desired value Press 9 to return to the main alarm programming menu and press again to return to the main display NOTE When alarm delay is set to OFF the Alarms will arm only when the programmed setpoint is reached 3 8 Chamber Alarm System When the incubator is switched ON or after any values have been reprogrammed the Alarm System is inactive until the setpoint values 0 1 are achieved after which the Alarm System is armed See Figure 8 Chamber Alarm System Flow Chart on the following page If temperature and or CO levels deviate more than the programmed amount the display flashes the audible alarm sounds and a message appears on the s
31. is opened and then slowing it down to produce a more accurate reading after the setpoint level is achieved CO Level amp CO Figure 7 Actual vs Displayed CO Levels COz Valve Close Point Displayed amp Actual P NS COzLevel zi Displayed COz Level Door Door o d I Closed pene ose 1 je 1 2 3 4 5 6 Time Minutes Tem perature 37 0 Deg C CO2 Level 5 0 Actual CO2 Level Hum idified to 90 RH Displayed CO2 Level Door Opening 10 45 Secs Displayed amp Actual CO2 Level 3 7 Programming the Alarm System 3 7 1 Setting High amp Low Temperature Alarms 1 Press the enter amp V keys simultaneously to enter the alarm menu The display will show A Press the enter key to display the High Temperature Alarm 4 77 5 The factory setting is the setpoint value 37 0 C 0 5 C If you wish to adjust the High Temperature Alarm use the A or V key The minimum setting however is 0 5 C from setpoint CO14S 0050 Galaxy 14 S CO Incubator User s Guide 23 Press the enter key to save the setting whether or not you have changed it Now the Low Temperature Alarm is displayed Z 75 5 The factory setting is the setpoint value 37 0 C 0 5 C Use the A or V key if you wish to adjust the value Press the enter key to accept the setting whether or not you have changed it 3 7 2 Setting the CO High amp Low Alarms l While you are still in the alarm
32. k Scientific User s Guide 40 2 Reach inside and disconnect the sensor by unplugging the connector be sure to grasp the white connector body not the wire leads 3 WARNING Never pull on the wire leads disconnect the white connector body 3 Using the sensor removal tool see Figure 10 unscrew the oxygen sensor by turning it counter clockwise Because the oxygen sensor contains lead be sure to dispose of it according to local regulations Figure 10 Oxygen Sensor Removal Tool 4 Using the sensor removal tool install the new oxygen sensor by turning it clockwise until it is finger tight Do not use excessive force or any metal tool 5 Reconnect the sensor by plugging the white connector body back in 6 Make sure that the sensor wires are inside the metal sensor tube to protect them from damage then press the rear access cover snugly back in place 7 After replacing the sensor humidify the incubator and allow it to stabilize overnight 8 Calibrate the sensor with reference to the atmospheric oxygen level see Section 7 2 4 7 2 8 Replacing the Filter Disc The hydrophobic filter installed on your Oxygen Control system helps prevent condensation from reaching the sensor see Figure 11 on the following page CO14S 0050 Galaxy 14 S CO Incubator User s Guide 41 Figure 11 Hydrophobic Filter amp Holder Assembly exploded view 1 HH i A DH OW i
33. ks as follows _ Inspect them to be sure there are no leaks or other damage 2 Check that the two stage pressure regulator valve and the inline pressure regulator valve are closed by trying to turn their knobs in a counter clockwise direction the knobs should be at the stop point 3 Place the inline pressure regulator close to the incubator 4 Securely attach the two stage pressure regulator to the nitrogen tank s outlet and to the inline pressure regulator s inlet with tubing securing both ends with clips 5 Connect the inline pressure regulator s outlet to the incubator s N inlet on the lower lefthand side of the equipment tray as you face the rear panel secure both ends with clips See important NOTE on the following page New Brunswick Scientific User s Guide 36 7 2 2 NOTE To add an automatic gas cylinder changeover instrument with the incubator turned off connect its inlet to the two stage pressure regulator s outlet and the changeover instrument s outlet to the inline pressure regulator s inlet with tubing securing the ends with tubing clips Setting Up Oxygen Control 1 NOTE Remove the red plastic protective cover from the hydrophobic filter cap making sure that the hydrophobic filter cap is not removed with it located in the rear wall of the incubator chamber Retain the cover for use when you clean the chamber Open the nitrogen gas supply from the tank S
34. menu see Step I of Section 3 7 1 above press the A key The display will show 24 A Press the enter key to display 7 5 5 The factory setting is the setpoint value 5 096 0 596 If you wish to adjust the High CO Alarm use the A or V key The minimum setting however is 0 5 C from setpoint Press the enter key to save the setting whether or not you have changed it Now the Low CO Alarm is displayed 277 5 The factory setting is the setpoint value 5 0 0 5 Use the A or V key if you wish to adjust the value Press the enter key to accept the setting whether or not you have changed it 3 7 3 Door Open Alarm When you open the door an alarm will sound after a preset time delay If you wish to adjust the time delay l In the alarm menu see Step 1 of Section 3 7 1 above press the A key the display will show zzz 7 reading across both displays Press the enter key and the A amp W keys to adjust the time as you scroll through the available choices you will see 4 74 45 44 75 amp 5 7 seconds then 7 Press the enter key to save the desired value 3 7 4 Alarm Duration The following steps will adjust the length of time during which all audible alarms will last l In the alarm menu see step 1 of Section 3 7 1 above press the A key The display shows F oc reading across both displays Press the enter key and the A amp W keys to adjust the Alarm duration as you scroll throug
35. ming the setpoint for CO to 0 0 to prevent CO from being injected into the chamber which would give a false reading Use a flow rate lt 0 5 liters minute to take the sample Keep the outer door closed Remember to reset the CO setpoint to the desired level after sampling We recommend that you perform a CO AutoZero prior to sampling see Section 3 5 3 We also recommend that you AutoZero the CO system once a month to ensure that the CO level is correct New Brunswick Scientific User s Guide 30 5 CLEANING amp DISINFECTING 5 1 Cleaning 1 Routinely clean the exterior of the incubator by wiping it over with a soft cloth moistened with soapy water 2 Rinse the soap from the cloth in clean water and wipe the exterior surfaces again CAUTION Be sure to use only approved cleaning fluids and materials Solvents chloride based cleaning substances and abrasive materials among others may cause permanent damage to the product surfaces Also be sure to wipe all surfaces dry leaving them free from any foreign particulates or fluids which could cause subsequent surface damage See the CAUTIONS in Section 5 2 5 2 Disinfecting The recommended disinfecting agent for use with the incubator is a solution of 7096 isopropanol iso propyl alcohol and 30 distilled water Be sure to follow appropriate safety regulations while you are using this solution WARNING As a routine precaution wear pr
36. monitoring of the chamber conditions A direct heat system utilizing a thermal heating element completely surrounds the incubator providing an even temperature within the chamber The independently heated outer door is designed to ensure an even distribution of heat thereby eliminating condensation on the door This system ensures a rapid controlled return to optimum chamber conditions after a door opening while also preventing any overshoot A solid state infrared sensor is used to control the level of CO providing excellent reliability and remaining unaffected by humidity The CO system has a semi automatic zero function AutoZero that provides a simple process to maintain an accurate level of CO within the chamber The 14 liter seamless deep drawn chamber and all internal components are manufactured from stainless steel The non tip shelves shelf racks and humidity tray are easily removed without tools for thorough cleaning and are capable of being sterilized Air circulation is achieved without the use of a fan eliminating ductwork a potential source of contamination simplifying cleaning eliminating vibration and reducing small sample evaporation within the chamber The outer shell of the incubator is manufactured from paint powder coated steel to give a durable corrosion resistant finish An independently controlled water tray at the bottom of the incubator allows a high uniform relative humidity while preventing conden
37. ner Glass Door P0628 6210 CO14S 0050 Galaxy 14 S CO Incubator User s Guide 45 8 DRAWINGS amp TABLES 8 1 List of Drawings Following is a key to the illustrations in this manual Figure Description Page 1 Shelf Rack Set Up left side 14 2 Shelf Rack Installation left side 14 3 Installing the Shelves 15 4 Shelf Racks Shelves amp Humidity Tray Installed 15 5 Rear Panel 17 6 Control Panel 18 7 Actual vs Displayed CO Levels 22 8 Chamber Alarm System Flow Chart 25 9 Oxygen Sensor Rear Access Cover 39 10 Oxygen Sensor Removal Tool 40 11 Hydrophobic Filter amp Holder Assembly exploded view 41 12 BMS Relay Contact Alarm Socket 43 82 List of Tables Following is a key to the tables in this manual Table Description Page 1 Accessories Provided 12 2 Utilities 12 3 Function Keys 18 4 Temperature amp CO Setpoint Ranges 20 5 Alarm Messages 27 6 BMS Relay Contact Alarm Socket 43 T Available Accessories 44 New Brunswick Scientific User s Guide 46 1 1 19 Oxygen Control 35 A Access Port 10 Accessories 44 Accessories Provided 12 Adjustable Feet 12 Installing the 13 Alarm Messages Recap of 27 Alarm Socket 42 Alarm System Programming the 22 Alarms Arming Time Delay 24 Duration of 23 Anti Slip Pads Installing the 13 Anti Slip Pads for Feet 12 AutoZero Frequency 29 Purpose of
38. otective gloves Be sure to adequately ventilate the work area as you are disinfecting to avoid the formation of potentially explosive alcohol vapors Protect all electrical connections from contact with the alcohol solution To best protect yourself your incubator and your work area follow these instructions 1 Program 0 0 CO and switch off the incubator then unplug the incubator from the power supply 2 Dampen a clean cloth with the alcohol solution and wipe down all external surfaces taking care to keep the alcohol solution from coming into contact with any electrical outlets or assemblies CO14S 0050 Galaxy 14 S CO Incubator User s Guide 31 3 Remove all of the shelves the humidity tray and the shelf racks CAUTION It is very important to ensure that no liquid is spilled onto the white porous CO sensor cover at the rear of the chamber see Step 4 Failure to use the protective cover could result in damage to the sensor and may affect your warranty 4 Place the black protective cover over the CO sensor 5 Youcan clean the humidity tray by rinsing it in sterile water wiping it down with the alcohol solution and then rinsing it with sterile water 6 Wipe down the inside of the chamber with the alcohol water solution and leave it to dry completely CAUTION Never use any of the following substances to clean the stainless steel or damage will result Sodium Azide Aqua Regia lodin
39. p 7 CO14S 0050 Galaxy 14 S CO Incubator User s Guide 37 7 To set the oxygen alarm levels Set the oxygen level alarms a press and hold the W key and press the enter key to enter the alarm menu b use the A or V key to scroll through the menu until 4 is displayed then press the enter key c The High alarm is displayed first use the A or V key to set the desired alarm level then press the enter key to store the change d the Low alarm is now displayed use the A or V key to set the desired alarm level then press the enter key to store the change 0 NOTE The alarm values cannot be set to less than 0 5 of the programmed value NOTE When using the incubator at low oxygen levels it is possible that the CO and O levels may not fully recover within the default alarm limit of 15 minutes after the door was opened This alarm delay time period can be modified in the alarm menu a enter the alarm menu as indicated in Step 7 above use the A or V key until or L is displayed b press the enter key c use the A or V key to increase the door delay value d press the enter key to store the change e press the key repeatedly until you return to the main screen 8 By finely adjusting the CO and N gas inlet pressures oxygen control can be tailored to achieve the programmed oxygen and carbon dioxide levels at approximately the same time 7 2 3 Operating Guidelines We recommend that you refer
40. perates in two sequential modes Mode 1 The over temperature cut out amp alarm activates and cuts power to the heating elements and the CO control valve The condition is shown on the display by the message ZZ F When the chamber temperature has fallen to the programmed setpoint the system changes to Mode 2 Mode 2 The control system then tries to maintain the chamber temperature at the programmed level by switching the heating elements on and off using an emergency control method that is however less precise The message L wil flash on screen to signal that an over temperature fault occurred and the incubator is being controlled by the emergency control system This message cannot be cancelled via the keypad Normal temperature control can be regained and the over temperature cut out amp alarm can be cancelled by reprogramming the temperature opening and closing the door or switching the incubator off and then back on If the problem persists the alarm will recur if this happens please contact your service representative or your distributor immediately 3 8 3 CO Control System Alarm This alarm only occurs if the CO AutoZero cannot reference the sensor signal to atmosphere If this is the case at the end of the AutoZero procedure the following message will appear LAZ ALY cEri FH LEd This means that the CO sensor is defective and requires replacement If this alarm occurs contact your service representative
41. ric levels During the sensor s lifespan the signal produced will slowly degrade until it is ultimately unusable For this reason we recommend that you reference the sensor to atmospheric oxygen levels on a monthly basis The sensor is automatically referenced to atmospheric oxygen levels when you enter the oxygen control menu To do this 1 First make sure the incubator has been humidified and left overnight to stabilize 2 Hold the A key then press the amp key the display will read Z cont 3 Press the A key until the display reads 4 7Z7 then open the incubator door 4 The display will begin a 60 second countdown timer zz 59 ez 54 cd 57 etc When the countdown period has elapsed close the incubator door 5 The oxygen control system will allow the oxygen sensor to stablize for ten minutes after the door is closed Once it has stablized the control system will reference the sensor to atmopheric oxygen level The reading is automatically adjusted to 19 796 which is the true reading taking into account the relative humidity in the chamber During the oxygen sensor reference to atmosphere procedure the control system measures the sensor output to estimate the remaining sensor life There are three possible outcomes The first is that the referencing procedure was successful and no further action is needed In this case the following scrolling message will be displayed 477 755 5 m 5er UH then Z
42. sation in other parts of the chamber The incubator incorporates a two level alarm system The chamber monitoring alarms are programmable and will alert you if temperature or CO have not recovered within a preset time after the door has been opened If it is not required this system can be disarmed The system alarms occur only if a system component problem has developed that requires user intervention to rectify The incubator also incorporates an over temperature safety system that operates independently from the main control system New Brunswick Scientific User s Guide 10 The Galaxy 14 S model contains many standard features usually seen as options such as a 25mm access port to allow for seamless integration of independent probes or other equipment through the chamber In addition NBS has included an RS232 port as standard on all Galaxy S series incubators This port will communicate with any computer through a hyperlink access or can be used to externally datalog the incubator through NBS software The incubator s direct heat system was designed with optimal use of laboratory space in mind it allows the most efficient internal volume for the footprint of the instrument In addition the incubator s top panel is specifically designed to support the weight of a second identical incubator stacked directly on top of the first CO14S 0050 Galaxy 14 S CO Incubator User s Guide 11 2 UNPACKING amp INSTALLATION 2
43. scscssssssssssssssssssssssscssssssssscsscssscesescsscscscesssscesesesssesssssssoses 32 6 1 CERTIFICATIONS 2 52 2 er oet tree te EBEN a uie revu teer erde 33 7 OPTIONS amp ACCESSORIES 00000000000000000000000000000000000000000000000000000000000000000000000 00000 35 7 1 OPTIONS AEE E E EA EN ee ie ET 35 7 2 Os CONTROL I 19 OPTION sai ae ea ee 35 New Brunswick Scientific User s Guide 7 2 1 Setting Up the Na Tank au ae ai 35 722 SONIS ED SCN C OHTFOLS eisen Stl Bently tee eet hadnt 36 7 2 3 Operating Guidelines nr een ner 37 7 2 4 Referencing to AImOSDIVEYTO Ae uada tts een 38 7 2 5 Replace Sensor Soon in alla 39 7 2 6 ReplaceSensor INOW sea A E A Eus 39 7 2 7 Removing amp Replacing OF Sensor ns 39 7 2 8 Replacing the Filter Discs ran 40 7 2 9 Troubleshooting the Oxygen Sensor iste anni 41 72 A0 Specifications 22 a i a ee dL 42 7 3 BMS RELAY CONTACT ALARM OPTION aneignen 42 TA AVAILABLE ACCESSORIES zit a dnas 44 DRAWINGS amp TABLES nungen 45 8 1 LIST OF DRAWINGS ohen ee ei ae nde eo E 45 53 JEITOF TABLES ana n eic R Te a AR RRRS 45 9 INDEX C E O E T EN E E E 46 CO14S 0050 Galaxy 14 S CO Incubator User s Guide 1 INTRODUCTION The Galaxy 14 S CO incubator is microprocessor controlled and designed to ensure accurate and reliable operation The incubator incorporates a simple door mounted touch sensitive keypad with two individual three digit LED displays that allow for easy programming and
44. structions after which the process is completely automatic Read the instructions that appear in the display the inner door if fitted must also be opened and closed according to the displayed instructions 4 Open the door for 60 seconds to completely degas the chamber A countdown is shown on the display 5 When the display instructs you to do so close the door An automatic 10 minute countdown will start to allow the chamber conditions to recover and the CO2 sensor signal to stabilize 6 At the end of the countdown the sensor signal will automatically be re referenced The incubator will reopen the CO valve to allow the CO level to recover to the setpoint value NOTE Pressing any key or opening the door during the AutoZero will automatically terminate the process and control of CO will restart The incubator is now ready for use New Brunswick Scientific User s Guide 22 3 6 CO Recovery After a door opening it should be possible for the actual CO level to recover to around 4 8 in 2 to 2 5 minutes The displayed CO level takes 30 to 45 seconds more to catch up with the actual level This is because it takes approximately 45 seconds from the time the gas enters the chamber to fully mix and become uniform inside the sensor The graph below Figure 7 shows typical results for door openings between 10 and 45 seconds The control system does this is by speeding the sensor reaction time when the door
45. tor User s Guide 33 Model 14 S Incubator Specifications Weight Crated 18 5 kg 40 8 Ibs Uncrated 12 5 kg 27 6lbs with standard features Grounded Electrical Supply 110 120V 50 60Hz Voltage or 220 240V 50 60Hz Power 500 watts Energy to maintain 37 C lt 0 06 kwh at room temperature between 20 25 C Storage Temperature 10 50 C 0 NOTE Factory calibration of the incubator is carried out at 37 C 5 0 CO with humidification in an ambient temperature of 20 25 C with no heat generating apparatus inside the chamber Software calibration adjustments may be required to optimize performance if the incubator is being used well outside these operating conditions Performance specifications may also be affected For advice on calibration adjustments and relevant performance specifications contact NBS Please be prepared with the model and serial number of your incubator and the complete details of your operating conditions 6 1 Certifications The CE Declaration of Conformity is reproduced on the following page New Brunswick Scientific User s Guide 34 Bj New Brunswick CE Conformity Certification Galaxy 14 S Declaration of Conformity Declare that the Galaxy 14 S CO Incubator Model Numbers CO14S 120 0 00 and CO14S 230 0 00 to which this declaration relates are in conformity to the following standards EN 61010 1 1993
46. ures 3a amp 3b Make sure that each shelf s anti tip groove is properly inserted onto each of the wire shelf rack guides For orientation the front edge of the shelf faces downward while the back edge of the shelf faces upward CO14S 0050 Galaxy 14 S CO Incubator User s Guide 15 Figure 3 Installing the Shelves Figure 3a Figure 3b 1 Humidity Tray slides in here see Fig 4 Level the incubator by adjusting the feet 1 Place a small level on the top shelf of the incubator 2 Adjust the leveling feet until the incubator is level and stable 3 Lock the leveling feet in place by tightening the locking nuts on each foot Install the humidity tray 1 Install the humidity tray between the lowest supports of the wire shelf racks see Figure 3b above and Figure 4 below 2 Ensure that the humidity tray is centered between the supports Figure 4 Shelf Racks Shelves amp Humidity Tray Installed Connect the CO gas supply New Brunswick Scientific User s Guide 16 1 Connect the incubator to the CO supply using the 6mm 14 inch plastic tubing with the installed HEPA filter by attaching the tubing between the two stage regulator or in line regulator and the matching CO inlet on the rear of the incubator Figure 5 below NOTE It is highly recommended that an in line regulator be used at the incubator s gas inlets 2 Use the tubing clips provided to eliminate CO le
47. zed biocide in the humidity tray Tests have shown that in addition to inhibiting bacterial growth in the tray this can reduce contamination on the chamber walls New Brunswick Scientific User s Guide 20 3 For IVF and other sensitive work we do not recommend the use of any biocide in the humidity tray To reduce the possibility of contamination every 10 14 days the humidity tray water should be changed and the humidity tray should be cleaned with a solution of 70 isopropyl alcohol and 30 distilled water s CAUTION Never leave water in the humidity tray if the incubator is switched OFF as this may cause damage to the CO sensor 3 4 Setting Temperature amp CO At this time program the desired Temperature and CO setpoints Temperature and CO may be set within the following ranges Table 4 Temperature amp CO Setpoint Ranges Parameter Available Setpoint Range Temperature 10 C to 50 C must be at least 4 C above ambient CO 0 2 to 20 To set the Temperature O Press the Programming 9 key Press the Up A or Down key until the desired value appears in the lefthand display Press the enter key to save the setpoint Now you can set the CO level AY To set the CO level 1 Press the programming 9 key 2 Press the up A or down V key until the desired value appears in the righthand display 3 Press the enter key to save the setpoint
Download Pdf Manuals
Related Search