ReadyToProcess WAVE™ 25 - GE Healthcare Life Sciences
Contents
1. The sensor is connected to the CBCU via an optical fiber cable DO measurement The DO level is measured with optical DO sensors preinstalled in Cellbag bioreactors of DOOPT type The sensor is connected to the CBCU via an optical fiber cable Three CBCU configurations are available e CO 0 and pH e Os Oyama DO CO 0 PH and DO The most suitable CBCU configuration will depend on the specific application ReadyToProcess Pump 25 The ReadyToProcess Pump 25 is a peristaltic unit incorporating two roller pumps for feed harvest and pH control The pump design makes tubing installation convenient and easy Tubing sizes from 0 5 mm 1 50 to 4 8 mm 3 16 internal diameter can be used to support flow rates from 0 07 to 100 mL min Flow rate is regulated by weight feedback from the scale and is controlled by automatic adjustments of the pump speed Manual adjustment of the flow rate is also possible Up to three pump units with all together six pump heads can be connected to the rocker Up to six pump roles can be selected from a set of 12 different possible options left acid base feed1 feed2 feed3 or harvest and right acid base feed1 feed2 feeds or harvest for the dual mode and seven different possible options acid base feed1 feed2 feed feed4 or harvest for the single mode Media handling and perfusion using calibrated pumps Calibration of pumps can be performed manually or automatically duri2. 3 C in the range of 15 C to 50 C within 5 C from calibration temperature ambient temperature 5 C to 40 C LOLL Max 10 C at ambient temperature 21 C Set point difference reduced by 1 C for each C increase in ambient temperature e g at ambient 25 C max set point difference is 6 C 1 When cultivating in a 50 L Cellbag bioreactor at maximum working volume of 25 L rocking speed and angle multiplied should not exceed 240 rom degrees For example if the rocking angle is set to 12 degrees the rocking speed should not be set higher than 20 rpm ReadyToProcess CBCU Gas flow control range Total gas flow accuracy reference flow set point Fast fill flow CO control range CO measurement accuracy at 5 CO CO control accuracy versus set point O control range O measurement accuracy O control accuracy versus set point pH measurement range pH control range OH measurement accuracy pH control accuracy versus set point DO measurement range DO measurement accuracy DO control range ReadyToProcess Pump 25 Pump flow rate range Pump flow accuracy Accumulated pumped volume accuracy Supported tubing dimensions Applications 50 to 1000 mL min 10 3 of read value mL min 3 L min 0 to 15 CO 0 5 CO when mixed only with air N 0 4 CO 0 to 50 O when mixed with N 21 to 50 O when mixed with air 0 6 1 of read value O wi
3. Gas flow mix and DO 29 0442 16 Disposable Cellbag bioreactors for Gas flow mix pH 29 0440 81 WAVE Bioreactor systems data file 28 9511 36 and DO Validation Support File UNICORN 7 0 28 9626 50 Pump 29 0320 03 ReadyToProcess WAVE 25 site preparation guide 29 0567 02 License 29 1281 16 ReadyToProcess WAVE 25 operating instructions 29 0095 97 License 29 1154 26 ReadyToProcess WAVE 25 system handbook 29 0095 98 License 29 1154 27 License 29 1154 56 Media no license 29 1280 20 29 0444 72 29 0444 73 8 29 0444 74 29 0444 75 z 29 0444 76 29 0444 77 29 0444 71 Fiber cable for pH and DO control 28 9841 89 1 29 1121 87 1 29 1125 25 t Depending on configuration one or two fiber cables are delivered with the CBCU unit t One delivered with the rocker GE Healthcare Bio Sciences AB Bj rkgatan 30 751 84 Uppsala Sweden www gelifesciences com upstream GE and GE monogram are trademarks of General Electric Company Bioclear Cellbag ReadyToProcess ReadyToProcess WAVE UNICORN and WAVE Bioreactor are trademarks of General Electric Company or one of its subsidiaries DeltaV is a trademark of Emerson Electric Co Windows and Windows XP are registered trademarks of Microsoft Corporation All other third party trademarks are the property of their respective owner Any use of UNICORN software is subject to GE Healthcare Standard Software End User License Agreement for Life Sciences Software Products A copy of this
4. Lid 20 Lid 50 CBCU Pump 25 Weight Rocker Tray 10 Tray 20 Tray 50 Lid 10 Lid 20 Lid 50 CBCU Pump 25 Power supply Power consumption Enclosure protective class Gas supply per CBCU External air supply 1 0 to 1 5 bar External CO supply 1 0 to 1 5 bar External O supply 1 0 to 1 5 bar Environmental aspects Operating ambient temperature range Operating humidity range UNICORN 6 3 2 or later version 404 x 205 x 560 mm 475 x 60 x 430 mm 740 x 70 x 480 mm 800 x 70 x 610 mm 475 x 230 x430 mm 740 x 245 x 480 mm 800 x 260 x 610 mm 2 6x 117 x 360 mm 275x115 x 280 mm 24 0 kg 4 5 kg 7 3 kg 9 5 kg 1 7kg 3 3 KO 3 9 kg 4 8 kg 3 8 kg 100 to 240 V 50 to 60 Hz 1500 VA IP 2 Normal use 1 3 L min 0 2 L min 0 7 L min Fast fill 3 5 L min 0 5 L min 1 7 L min To GC 20 to 80 relative humidity noncondensing ReadyToProcess WAVE 25 rocker Rocking speed control range Rocking angle control range Rocking motion control range Medium weight control range Scale absolute accuracy Scale left right absolute accuracy dual mode Temperature sensor Temperature measurement range Temperature measurement accuracy Temperature control range Temperature control accuracy excl measurement error Temperature set point difference dual mode 2 to 40 rom to 12 15 to 100 0 2 to 25 kg 0 050 1 of load kg 0 1 6 of load kg Pt100 Class A 2 C10 50 C 0
5. a bioreactor run can be defined in a method The Method Editor module is used to create edit save and work with methods An existing method can be changed to simplify the editing process Individual changes can be saved for later use on systems having the same instrument and component configuration System control The System Control module is used to connect to the system as well as to start view and control a run Fig 8 Default parameter values for an instrument can be viewed and edited in the System Settings dialog before a run is started Or during an ongoing run using manual instructions It is also possible to connect to other systems The user friendly and intuitive Process Picture pane enables manual interaction with the system and provides status of the run parameters Data is shown in the process picture but can also be viewed as curves in the Charts tab Curves and information about the run are saved in a database which can be opened in the Evaluation module The default view shows the curves most commonly used The user may customize which curves to display and the color and style of the displayed curves SHeh ss Gy pH 0 00 Safon 0 00 uma fh gas ta J lll Fig 8 Monitoring and controlling all aspects of the culture is managed through the user friendly interface Evaluation In the Evaluation module content of the result files can be viewed analyzed and compiled as reports Reports can be customized s
6. independently in two separate 5 L cultures using the dual functionality ambient temperature 22 C 29 0566 95 AD 3 amp Volume 2 L Volume gt S 10 10 a Il 9 9 2 8 8 2 6 D 6 E lt 5 lt 5 5 4 4 s 3 5 E 2 s 2 10 12 14 16 18 20 22 24 26 28 30 10 12 14 16 18 20 Rocking speed rpm o 10 10 9 9 2 8 8 a n 7 im l 8 6 D6 E i lt 5 5 4 4 S 3 3 5 2 2 10 12 14 16 18 20 22 24 26 28 30 10 12 14 16 18 20 Rocking speed rpm Rocking speed rpm Rocking speed rpm 6L Volume 10 L 10 100 9 95 8 90 s 7 85 x 2 6 80 5 75 4 70 3 65 2 a 60 22 24 26 28 30 10 12 14 16 18 20 22 24 26 28 30 Rocking speed rpm Fo O Oxygen transfer coefficient Angle MSN S Ur Oro dr CO WO 22 24 26 28 30 10 12 14 16 18 20 22 24 26 28 30 Rocking speed rpm Fig 7 4D contour plot of the oxygen transfer coefficient at the highest 100 and lowest 15 rocking motion parameter settings Mixing and gas transfer The adjustable rocking parameters are speed angle and motion The parameter settings in combination with the cell culture volume affect the mixing and gas transfer rate in the Cellbag bioreactor The speed parameter determines the number of rocking cycles per minute and the angle parameter relates to the tray s degree of tilting at the turning points The motion parameter determines the acceleration profile The lowest motion parameter se
7. Data file 29 0566 95 AD ReadyToProcess ReadyToProcess WAVE 25 The single use ReadyToProcess WAVE 25 bioreactor system is a reliable and intuitive cell culture device for working volumes up to 25 L Fig 1 The system is based on a well known rocking technology that provides mixing and aeration to the culture which is maintained in an inflated disposable Cellbag bioreactor cultivation chamber With ReadyToProcess WAVE 25 the rocking technology is enhanced with features such as advanced sensors and intelligent control strategies System operation is easily managed via the intuitive and user friendly UNICORN system control software The system is designed for fast installation and convenient handling and delivers a reliable and accurate performance suitable for research process development and manufacturing operations ReadyToProcess WAVE 25 offers the following benefits e Robust and reliable performance e Intelligent and accurate process control e Designed for ease of use e Flexible operations with possibility of use in single or dual culture mode e Suitable for manufacturing in a regulated environment System benefits Robust and reliable performance ReadyToProcess WAVE 25 is designed to make hardware consumables and software work together in an integrated manner The system has been tested in real applications for reliable performance To decrease the risk for data losses the system comprises two mirr
8. Standard Software End User License Agreement is available on request Cellbag bioreactors with integrated optical sensors are sold under a sublicense from Sartorius Stedim Biotech under US patent numbers 6 673 532 7 041 493 and or its foreign equivalents 2013 2014 General Electric Company All rights reserved First published Jun 2013 All goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them A copy of these terms and conditions is available on request Contact your local GE Healthcare representative for the most current information GE Healthcare UK Limited Amersham Place Little Chalfont Buckinghamshire HP7 9NA UK GE Healthcare Europe GmbH Munzinger Strasse 5 D 79111 Freiburg Germany GE Healthcare Bio Sciences Corp 800 Centennial Avenue P O Box 1327 Piscataway NJ 08855 1327 USA GE Healthcare Japan Corporation Sanken Bldg 3 25 1 Hyakunincho Shinjuku ku Tokyo 169 0073 Japan For local office contact information visit www gelifesciences com contact 29 0566 95AD 12 2014
9. al After a run data is stored in a UNICORN database on an external computer For facilitated integration into a larger manufacturing operation the rocker also contains software for enabling direct communication with the system via an open platform communication OPC link ReadyToProcess CBCU The ReadyToProcess CBCU gas mixer is a compact unit with multiple functions The unit is primarily used for providing gas to the culture and for monitoring of pH and DO The unit contains a mass flow controller sensors for gas pressure and O and CO concentrations and transmitters for pH and DO Table 1 Table 1 ReadyToProcess CBCU gas mixer overview Feature CO 0 air mix controller Description Depending on the configuration air is mixed with CO and or O according to the set points Nitrogen may be used instead of air for maintaining a low oxygen environment for near anaerobic applications Gas flow controller The gas mix flow is measured and controlled by a mass flow controller MFC A correct volumetric flow is achieved by compensation for CO concentration Quick filling of gas into the Cellbag bioreactor enabled by the fast fill function Significantly reduces start up time An alarm will inform the user if pressure sensors detect under or overpressure at the gas inlets or within the Cellbag bioreactor DH measurement The pH is measured with optical pH sensors preinstalled in Cellbag bioreactors of DHOPT type
10. aved and printed 29 0566 95 AD 5 Alarms notifications and data logging The UNICORN software allows easy access to all operations data and alarm conditions A dynamic graphical user interface shows the real time status of the run while data are automatically saved From the UNICORN software the system is easily configured to trigger an alarm when certain conditions are met Individual deviation alarms can be set for all essential parameters heating gas flow weight O CO pH and DO The regulation of alarm sensitivity and delay is defined by the user If such an alarm condition is triggered the part of the system generating the alarm is highlighted in the graphical user interface and an alarm dialog is displayed The alarm dialog displays information about the alarm such as date and time of occurrence as well as a help text describing the cause of the alarm and how to solve the problem The system is easily configured to send email notifications of alarms and errors Regulatory readiness The ReadyToProcess WAVE 25 system is suitable for biomanufacturing of regulated products under various Quality Management Systems The use of UNICORN software in a 21 CFR Part 11 and GAMP 5 compliant manner enables use of the system in a regulated environment Individual user access permissions can be set and individual users are password protected Active processes can be locked to enable unattended operations without the risk of unautho
11. est lines Optical pH sensor embedded in bag Cellbag rod Optional tubing Optional tubing Needleless sampling port Fig 9 Presentation of typical Cellbag bioreactor fittings Table 2 Possible combinations of Cellbag bioreactors and trays when culturing in single or dual mode Cellbag Bioreactor Tray bioreactor culture Single Dual size L volume L culture mode culture mode 2 0 3 to 1 Tray 10 Tray 20 Tray 20 10 0 5 to 5 Tray 10 Tray 20 Tray 20 20 1 to 10 Tray 20 Not applicable 22 1 to 10 Tray 50 Tray 50 50 51025 Tray 50 Not applicable All Cellbag bioreactor sizes are available with internal perfusion filter for perfusion cultures and or can be equipped with single use optical pH pHOPT and DO DOOPT II sensors 1 Cellbag bioreactors with optical sensors require 300 mL minimal working volume Depending on application and configuration it might be possible to cultivate below the recommended minimum volume However it is highly recommended to stay above this volume for applications that require high agitation and pH and DO control The temperature pH and DO sensors need to be submerged in liquid throughout the complete rocking cycle to function correctly System specifications System specifications are listed in Table 3 Table 3 ReadyToProcess WAVE 25 system specifications General system specifications Control system Dimensions W x H x D Rocker Tray 10 Tray 20 Tray 50 Lid 10
12. g the run and then stored on the database server 6 29 0566 95 AD Because results and instructions are saved locally on the embedded drives a run can continue even in the event of a network communication failure UNICORN software can be integrated in a supervisory control and data acquisition SCADA system such as the Emerson DeltaV control system using the UNICORN OPC server Please contact your local representative for further information Cellbag bioreactors Presterilized Cellbag bioreactors are single use bags for the noninvasive mixing of culture medium and cells during cultivation Fig 9 Cellbag bioreactors require no sterilization or cleaning steps The disposable bioreactors provide a suitable environment for cell growth while minimizing the cross contamination risk The bags are manufactured from multilayer laminated clear USP Class VI plastics and are easily connected to the full suite of ReadyToProcess cell culture purification and fluid handling products Table 2 shows possible combinations of Cellbag bioreactors and trays when culturing in single or dual mode Cellbag bioreactors are available in two films Bioclear 10 and the low antioxidant Bioclear 11 For more information about Cellbag bioreactors see data file 28 9511 36 Optical DO sensor tte tetttetitteeteteeteeeetereteeetteeetteeesteeeeteesteeetieeen embedded bac Air outlet filter Optional tubing Air inlet filter Inoculation harv
13. mp units control liquid addition and or removal for example to enable tailored feeding strategies base addition or perfusion in the two parallel cultures Lid Graphical user interface ReadyToProcess Pump 25 Peristaltic pump Client computer Tray ReadyToProcess WAVE 25 rocker ReadyToProcess CBCU Installed with UNICORN software Rocker temperature sensors Gas flow mix controller Can control up to three scale embedded controller pH and DO sensors ReadyToProcess WAVE 25 systems and dual hard drives System setup and integration of subunits TT ee Si SS i eee i ti had a it ri ReadyToProcess WAVE 25 rocker ReadyToProcess Pump 25 l e Acid base for pH control e Rocking e Feed harvest for perfusion or medium addition e Weight e Up to three pump 25 units can be connected measurement I 1 i e Temperature UNICORN software measurement e Administration a ReadyToProcess CBCU e System control e Gas flow mix e Method editor e pH measurement Evaluation Controller with dual hard drives amp DOmenciremeni Up to two CBCU can be connected LAN OPC oe Se petiole mais lt a UNICORN 5 SCADA database not included k ee aa a i UNICORN
14. n A snap lock mechanism allows Cellbag bioreactors to be correctly installed and rapidly changed Fig 4 To protect light sensitive components of the culture medium and to prolong the life of the optical sensors lids are available for all tray sizes Snap locks Fig 4 The tray has an easy lock mechanism for convenient and proper attachment of Cellbag bioreactors Temperature control Efficient and evenly distributed heating is provided by the tray heater plate The temperature control is managed by sensors that are integrated in the rocker base unit to enable reliable temperature measurement and to minimize the need for calibration when changing trays Fig 5 To minimize the risk for overheating heating is only enabled when the rocker is in motion For an accurate stable and fast temperature control the heater power output is automatically adjusted based on the Cellbag bioreactor size and content weight Temperature control in two separate Cellbag bioreactor cultures when using the dual functionality is shown in Figure 6 DEA F Temperature n 3 sensors A off Fig 5 Integrated temperature sensors for convenient handling and reliable temperature control 40 WN Ga Heating left culture Temp set point left culture Heating right culture Temp set point right culture Temperature C PO WN on O DO O 0 50 60 90 Time min Fig 6 Temperature is accurately controlled
15. ng an ongoing perfusion process Automatic calibration is possible for flow rates above 3 5 L d and is easily managed by entering the tubing diameter in the UNICORN control software Automated pH control To minimize fluctuations in pH during adjustments with CO base or acid base the flow rate of the acid base pumps needs to be rigorously controlled The ReadyToProcess WAVE 25 system uses combined information from the load cells the current process pH value the pump tubing diameter and the acid base molarity for calculation of the needed flow rate during the run Automation of process parameters in pH and DO control The UNICORN software automatically sets the desired PID control parameters based on Cellbag bioreactor size and gas flow set point The system also adapts the parameters during the run for optimization to reach a new set point or to maintain a current set point Proportional integral derivative UNICORN software UNICORN system control software consists of four modules Administration Method Editor System Control and Evaluation A comprehensive help tool is also included Administration The Administration module is used to administer all functions of the UNICORN software Available functions include user and email setup controlling access to groups and network users defining and editing system properties database management and logging records of usage and activity Method editor Instructions to control
16. ored solid state drives for data storage Intelligent and accurate process control The system is equipped with advanced sensors and automated controllers for temperature liquid handling pH and dissolved oxygen DO The system dynamics have been gelifesciences com Fig 1 ReadyToProcess WAVE 25 bioreactor system can be operated in single or dual culture mode methodically characterized and the results used to create a library of predefined control parameters tailored for different cultivation volumes The system can automatically determine suitable parameters for a run a feature that facilitates fast start up and enables accurate and stable control Easy to use ReadyToProcess WAVE 25 system is designed for ease of use Cellbag bioreactor attachment is straightforward and system operation is conveniently managed with the UNICORN software with menus and process pictures that are easily accessible and interpretable The harvest operation is facilitated by an innovative tilt function that omits the need for heavy lifting Flexible operations with possibility of use in single or dual culture mode With ReadyToProcess WAVE 25 one or two cultures can be run at the same time with high control accuracy When dual mode is used two cultivations can be run using the same or different bag sizes and or working volumes Parameters such as temperature pH and DO can be controlled independently in the two Cellbag bioreactors Separate pu
17. rized interference All records are stored in a single unalterable database including results and extended run documentation Additional validation support includes comprehensive documentation on control system validation as well as 1Q OQ services The IQ OQ offers proven test procedures verifying that the equipment has been installed in accordance with system drawings and specifications The IO OO also assures that the system operates as specified in the design satisfying functional requirements The IO OO protocols enable quality assurance and allow regulatory reviewers to verify that all functional testing of the quality critical equipment and components including the requirements of 21 CFR Part 11 has been performed and documented Available validation support documentation includes e Detailed description of the development model used for UNICORN software e 21CFR Part 11 system assessment in checklist format e Audit report and 21 CFR Part 11 conclusion on functionality by an external independent expert Networking capabilities UNICORN 7 operates in Windows 7 environments and the network ability allows real time control from a remote or local computer Communication is Ethernet based and each instrument is controlled by a built in instrument server One database can be connected to 32 systems and up to three instruments can be controlled simultaneously from one UNICORN client Results are saved locally in the rocker durin
18. software E mail At home or within notifications enterprise network Fig 2 ReadyToProcess WAVE 25 system overview The System Control module on the client computer is used to start and monitor the cultivation process One UNICORN client can control up to three ReadyToProcess WAVE 25 systems simultaneously SCADA supervisory control and data acquisition OPC open platform communications LAN local area network 2 29 0566 95 AD a Suitable for manufacturing in a regulated environment ReadyToProcess WAVE 25 is designed to meet the demands and standards that are required in a regulated environment The documentation is extensive and includes material certificates system specifications installation and operational qualification IO O0 protocols and a detailed user manual The UNICORN software Is suitable for use in a manner that complies with 21 CFR Part 11 and Good Automated Manufacturing Practice GAMP 5 System overview ReadyToProcess WAVE 25 system components The ReadyToProcess WAVE 25 system consists of a rocker CBCU gas mixers and pumps all operated by the UNICORN software installed on a client computer The rocker is the main unit and is used together with a tray and a disposable Cellbag bioreactor When operated in dual culture mode culturing can be performed in two separate Cellbag bioreactors simultaneously although controlled separately The rocker has multiple functions amongst them temperature control c
19. thin 0 to 50 O when mixed only with air N 0 6 O pH 4 5 to 8 5 pH 6 0 to 8 0 0 05 pH within 0 25 pH from offset calibration pH 0 1 pH within 0 25 to 0 5 pH from offset calibration 0 05 pH 0 to 250 air saturation 5 air saturation excl atmospheric pressure variations 0 to 100 air saturation 0 1 to 144 L d 0 07 to 100 mL min 0 1 5 of read value mL min after calibration 10 of measured volume i d 0 5 to 4 8 mm 1 50 to 3 16 wall thickness 1 6 mm 1 16 ReadyToProcess WAVE 25 is designed for a variety of cell culture applications involving mammalian insect and plant cells e Batch culture e Fed batch culture e Perfusion culture e Cultivation of adherent cells 29 0566 95 AD 7 Ordering information Product ReadyToProcess WAVE 25 ReadyToProcess CBCU pH ReadyToProcess CBCU DO ReadyToProcess CBCU Full ReadyToProcess Pump 25 UNICORN 7 0 WrkStn pure BP exp UNICORN 7 0 Remote UNICORN 7 0 Dry UNICORN 7 0 Evaluation Classic UNICORN 7 0 DVD pack Tray 10 Tray 20 Tray 50 Lid 10 Lid 20 Lid 50 Filter heater Bag sensor adaptor 2 5 m assembly Tube kit CBCUt Adjustable foot wrench One delivered with the rocker additional needed for dual functionality Description Code number Related literature Code number Rocker 28 9880 00 UNICORN 7 0 Manual Package 29 1277 95 Gas flow mix and pH 29 0442 13 UNICORN 7 0 control software data file 29 0567 02
20. tting 15 gives an almost constant speed throughout the rocking cycle whereas the highest setting 100 gives a faster speed in the middle of the cycle and slower at the turning points Whereas oxygen transfer increases with increasing rocking speed and angle the rocking motion pattern only has a minor effect on the oxygen transfer rate Fig 7 Weight measurement Load cells integrated in the rocker provide accurate and continuous weight measurement of the culture and eliminate the need for external scales In Dual mode separate weight measurements are provided for each Cellbag bioreactor The load cells communicate with the system control function for proper media handling and tailored regulation of pH and temperature The weight measurement functionality is also used for automatic calibration of the pumps in perfusion cultures An adjustable foot enables equal weight distribution between the load cells also on nonlevel surfaces for increased precision of the weight measurement Data storage and communication Data and control parameters from the cell culture run are stored on two independent mirrored solid state drives integrated in the rocker Thus once cultivation has been started and data from the instrument modules have been collected the run can continue without the need for a network connection The use of two solid state drives enables culturing without losing any data even if only one drive is 4 29 0566 95 AD function
21. ulture mixing and weight measurement The CBCU contains a gas mixer that delivers gas of a defined composition to the culture and is used together with the optical sensors of the Cellbag bioreactor for online control of culture pH and DO Liquid is delivered accurately to the culture by ReadyToProcess Pump 25 The pump unit has a flow range covering multiple applications from additions of feed and base to perfusion culturing Up to two CBCU gas mixers and up to three pump units can be connected to ReadyToProcess WAVE 25 An overview of the ReadyToProcess WAVE 25 system is shown in Figure 2 The individual components are described in detail below ReadyToProcess WAVE 25 rocker The rocker is the main hardware unit of the system and provides mixing through the rocking reliable temperature measurement from integrated sensors and accurate weight measurement from integrated load cells The ergonomic design makes handling convenient and easy The ability to handle the bioreactor in a tilt position facilitates sampling and harvest Fig 3 The minimal footprint facilitates placement when space is limited A Fig 3 A The ergonomic design makes activities such as sampling and harvest convenient and easy B ReadyToProcess WAVE 25 in tilt position Trays and lids Trays are available in three different sizes for culturing of up to 5 L 10 L and 25 L respectively The trays are easily attached to the rocker when in the tilt positio
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