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VStack User Manual - Agilent Technologies

1. 25 installing Softwares te ced sos 25 Loading and Releasing a 28 Placing a Plate sry EAr erbe Daae id ete eate ets at ts a 30 Verifying Network 31 Identifying VStackS 32 Changing the Device ld 33 Deleting 34 Chapter 3 Configuration and Manual Operation Opening Diagnostics for a Specific 5 36 Selecting a Plate 38 Adding a Plate Type To the Labware 39 TESUME Plate Handling 4 5 5 0 3 230 7T Bed E OE d Pe conie E ades 40 Adjusting the Grip Pressure 41 Adjusting the Plate Specific Grip 42 Viewing Sensor lt 5 43 Configuring Plate Orientation Checking 45 Operating the VStack 52 Displaying d NeSSSBOL osuneen sae use Ep o sve uL el pes ao 54 About lt 54 Changing the Presentation 56 About the Moton Pages card qe Ed MUS cd a O NE
2. File Version Internal Name Language Original File name Product Name Product Version Apply Cancel Lab Automation System Documentation Other Documentation Content Summary Chapter 1 Introduction VStack User Guide The VStack is never used as a stand alone device it is always incorporated into a larger lab automation system that requires its own documentation If your lab automation platform is the 11 BioCel read this guide in combination with the BioCel User Guide If your lab automation platform is a third party system read this guide in combination with the documentation that is available for your system In addition to this guide read Getting Started and parts of the Database and Security User Guide Getting Started gives Velocity11 support information explains how to use the online help versions of Velocity11 documentation and provides general safety guidelines The Database and Security User Guide gives information about the labware editor This guide covers Q How to install the VStack hardware How to connect the VStack to the network How to install and use VStack ActiveX software Maintenance and troubleshooting DODO ActiveX commands which allow control of the VStack by third party systems Chapter 1 Introduction VStack User Guide Computer and Laboratory Requirements Computer Requirements Laboratory Requirements DODO d Q
3. Grip Offset Is a of the VStack It is actually a proxy of the labware editor s Stacker gripper Proxy offset property meaning that if you change the value of the plate specific grip offset the same change is made in the labware editor for that particular type of plate The reverse is also true For more information about the labware editor see the Database and Security User Guide Procedure To change the plate specific grip offset 1 Place at least two plates in the rack 2 Load the rack onto the VStack For more information see Loading and Releasing a Rack on page 28 3 Open the VStackBioNet Diagnostics software For more information see Opening Diagnostics for a Specific VStack on page 36 Select the appropriate plate type Load the rack by clicking Release Stack 6 Look at the grippers from the side through the rack If the grip position is where you want it do not change the gripper offset If it is not continue with step 7 Click Release Stack to move the plate out of the grippers 8 Change the Plate specific grip offset value which is in millimeters and click Apply 0 4 Plate specific grip offset Enter a number higher than the set value to grip the plate above the current grip point Enter a number lower than the set value to grip the plate below the current grip point 9 Click Release Stack to load the plate into the grippers Chapter 3 Configuration and Manual Operation 43 VStack Us
4. PC running Windows 2000 or XP At least a Pentium 166 processor 32 MB RAM 10 BaseT Ethernet port Bench space 8 5 inches x 8 0 inches x 7 5 inches 21 60 cm x 20 30 cm x 19 05 cm Lx W x H Electrical supply 110 240 VAC 50 60 Hz Air supply 50 80 psi at 1 cfm minimum 15 psi DAMAGE HAZARD Operating the VStack at the wrong voltage may seriously damage the instrument IMPORTANT Alllabware including deepwell and PCR plates used in the VStack must conform to the SBS microplate standards Please visit www sbsonline org msdc crevs html for current SBS standards or contact your labware s manufacturer Chapter 1 Introduction General VStack Hazard Warnings Before installing and using the VStack Before installing and using the VStack make sure you are aware of the potential hazards and understand how to avoid being exposed to them You must be properly trained in the correct and safe installation and operation of the device EU installations only Be aware that the VStack is considered partly completed machinery that is required to be installed with other equipment such that the fully assembled machinery complies with the essential health and safety requirements EHSRs of the Machinery Directive 2006 42 EC Intended product use Injury hazards INJURY HAZARD Do not remove the VStack exterior covers or otherwise disassemble the robot Doing so can expose you to hazards that could cause serious injury
5. Base Display Gripper Load Release button Plate stage Rack Chapter 2 Installation 13 VStack User Guide The main parts of the VStack are described in the following table Description Controls the pressure of the air that is used to close the grippers The two similar controls to the left and right are flow controls that affect the speed with which the grippers move in and out You should not need to adjust the flow controls Base of the VStack which has dowel pin holes and screw holes for attaching the VStack to a custom prepared lab bench or base plate Displays a text string when the void DisplayMessage ActiveX method runs You can run this method manually to test the display through the VStack Diagnostics software For more information see Displaying a Message on page 54 This method can also be called during a run by the run controlling software For example on the Velocity11 BioCel VWorks uses this method to deliver the name of the type of plate that should be loaded into each VStack When loaded grips the bottom microplate holding up the rest of the stack of plates During operation it releases the bottom plate to the plate stage when dispensing plates and grips the plate on the plate stage when receiving plates from the robot Pressing the button loads or releases a plate When the plates are released you can remove the rack Moves up and down to move plates between the rac
6. For more information see Opening Diagnostics for a Specific VStack on page 36 2 Click Move Here for the Presentation teachpoint This moves the plate stage to the presentation position Presentation 200 f 3 Move the plate stage up or down using the jog controls The increment control allows you to move the plate stage up or down in large or small moves Adjust the increment control to the desired increment and click Up or Down to move the plate stage r Jog Controls i In Motion eo Increment i Busy e o 4 When the plate stage is at the desired height click Teach Here Presentation 2 00 E i Move Here 5 At the Modify Teach Point dialog box click OK to accept the changes or Cancel to cancel the operation and return to the previous screen Chapter 3 Configuration and Manual Operation BT VStack User Guide Modify Teach Point x j amp re you sure M The Presentation teachpoint is now saved About the Motor Page Motor Page IMPORTANT The Motor page is intended for use during Screenshot manufacture and you should not use it without supervision by a Velocity119 service representative If you accidentally change a value refer to the screenshot below for the default value to change it back to Diagnostics Motor Empty Full NEN his m High Travel Limit Ki p E Low Travel Limit i
7. network card included in the kit b Follow the instructions included with the card to install it 2 Setthe IP address of the card a Click Start gt Settings gt Network and Dial up Connections b Right click the icon for the new local area connection probably Local Area Connection 2 and click Properties File Edit View Favorites Tools Advanced ay sax 2 re Search Folders e 2 XxX i Address network Connections dge New Connection Wizard Disable Status Repair Bridge Connections Create Shortcut Delete Rename Properties c Double click Internet Protocol TCP IP Chapter 2 Installation 21 VStack User Guide BITE Area Connection Properties A File and Printer Sharing for Microsoft Networks M QoS Packet Scheduler d Enter the values shown in the following screenshot Internet Protocol TCP IP Properties C Obtain DNS server address automatically 9 e Click OK f Close the Network and Dial up Connections window Chapter 2 Installation VStack User Guide For more information about the IP address of the network card see About IP Addresses on page 18 Installing the DHCP Server Introduction This section assumes that you have purchased the DHCP Server Kit from Velocity11 The DHCP server automatically assigns IP addresses to VStacks when they are turned on so they can be
8. 384 Auto wash Reservoir Normal Lidded Sealed Orientation Checking 3 Select one of the following options Option Description Normal The plates you plan to use are not sealed and have no lids Lidded The plates have lids Sealed The plates are sealed 4 If you want the VStack to sense the orientation of the plate select the Orientation Checking check box If this option is not selected the plate stage still moves to the Orientation teachpoint during upstack and downstack operations but the orientation is registered as correct regardless of the actual orientation of the plate 5 Click Apply to save the changes If you are setting up the VStack for the first time read through the remainder of this chapter and check all configuration settings before testing plate handling If your VStack is already set up and you are just checking plate handling after making an adjustment or selecting a new plate type follow the procedure in Testing Plate Handling on page 40 next Chapter 3 Configuration and Manual Operation 39 VStack User Guide Adding a Plate Type To the Labware Database Background For correct operation of the VStack the software controlling the robot must be given the dimensions of the type of plate you intend to use This information is held in the labware database If the type of plate you want to use is already defined in the database you can ignore this section If the type of plate you want
9. Configuration and Manual Operation 55 VStack User Guide Teachpoint Definition Default Value mm Presentation The height at which the stage presents a plate for 2 00 pick up by a robot IMPORTANT Do See Changing the Presentation Teachpoint on not change this value page 56 for information about changing this value for VStacks used on the BioCel Teachpoint Values Teachpoint values are displayed in the Teach Points group box of the VStackBioNet Diagnostics window m Teach Points Stack Release 100 00 Orientation Sensor 50 00 Presentation 2 00 Chapter 3 Configuration and Manual Operation VStack User Guide Changing the Presentation Teachpoint Introduction Procedure The Presentation teachpoint specifies the height above the home position at which the VStack presents plates This teachpoint should only be changed if a particular configuration requires a Presentation teachpoint that is different from the 2 00 mm default IMPORTANT Do not change this value for VStacks used on the BioCel Note that the labware editor includes a property called Presentation offset This property is used by the BioCel s VWorks For more information see the Database and Security User Guide Note When controlling the VStack from other computers the default teachpoint will appear unless it is changed on each computer To change the Presentation teachpoint 1 Open the VStack Diagnostics software
10. Mo Integrator limit 10 ho Output limit 253 53 Deadband compensation 0 0 Velocity mm s 200 150 Acceleration mm s 3000 1000 Servo rate 1 Position error limit mm 1000 1000 58 Chapter 3 Configuration and Manual Operation VStack User Guide Maintenance and Troubleshooting This chapter Q Describes the common VStack errors and their possible causes and solutions Gives a maintenance schedule that you can use to keep the VStack in excellent working condition Note The error messages described in this chapter are generated by the VStack ActiveX If you are using the VStack with the BioCel s VWorks the error messages you receive may be slightly different Chapter 4 Maintenance and Troubleshooting VStack User Guide VStack Error Messages Introduction Labware Definition Errors Cannot Perform Operation Without First Loading Rack In this section the text of the error message is written as a heading which is followed by a description of the error possible causes and solutions If the labware definitions are not set appropriately for the plate you are using you may get an error of some kind depending on the specific problem If you receive a VStack error make sure you consider the labware settings in the labware editor The VStack failed to sense a rack when you clicked the Upstack button even though a rack was present eo Cannot perform ope
11. Velocity11 product name changes Velocity11 product name Changes to Access2 Automated Microplate Loader Automated Centrifuge Loader Element Automation System BioCel 900 System IWorks Device Driver Programming Interface VWorks DCL Interface PlatePierce Seal Piercing Station Microplate Seal Piercer VCode Barcode Print and Apply Station Microplate Barcode Labeler Velocityll Robot 3 Axis Robot VHooks Integration Interface VWorks Hooks Interface VPrep Pipetting System Vertical Pipetting Station VSpin Microplate Centrifuge Microplate Centrifuge VStack Labware Stacker Labware Stacker New contact information Documentation feedback documentation automation agilent com Technical Support 1 800 979 4811 or 1 408 345 8011 service automation agilent com Customer Service 1 866 428 9811 or 1 408 345 8356 orders automation agilent com European Service 44 0 1763208826 euroservice automation agilent com Web http www agilent com Labware Rack Handling Guide This guide explains how to handle the labware racks safely The topics are About the labware racks Carrying the racks Lifting the racks Loading labware into the racks Accessing product user documentation Contacting Velocityll About the labware racks The BenchCel Microplate Handling Workstation and the VStack Labware Stacker use labware racks to store the s
12. and damage the Labware Stacker INJURY HAZARD Using controls making adjustments or performing procedures other than those specified in the user guide can expose you to moving parts and hazardous voltage Agilent Technologies products must only be used in the manner described in the Agilent Technologies product user guides Any other use may result in damage to the product or personal injury Agilent Technologies is not responsible for any damages caused in whole or in part by improper use of the products unauthorized alterations adjustments or modifications to the products failure to comply with procedures in Agilent Technologies product user guides or use of the products in violation of applicable laws rules or regulations Except as otherwise expressly provided in Agilent Technologies product user guides any alteration adjustment or modification to the products will void the product warranty The VStack is not intended or approved for diagnosis of disease in humans or animals You assume full responsibility for obtaining any regulatory approvals required for such use and assume all liability in connection therewith INJURY HAZARD Never lean against the VStack INJURY HAZARD Keep far enough away from the VStack while it is in motion so you do not catch your hands clothing jewelry and so on in the mechanism INJURY HAZARD Do not start the VStack controlling software while you are near the VStack s plat
13. choose a place that allows access on all sides for cleaning and maintenance This procedure assumes that you have already prepared your bench area and have had the necessary holes drilled and screw threads tapped in your benchtop or mounting plate Note Velocity11 does not provide dowel pins or screws You will need to obtain pins and screws that are the correct length for your particular lab setup To mount the VStack 1 Measure your prepared mounting holes to make sure that they will accommodate the VStack Dimensions in the diagram below are in inches O 1 4 dowel pin hole x3 g M6 flathead x4 9 3 918 6 3 500 E 1 909 500 o 2 092 2 500 2 092 2 500 16 Chapter 2 Installation VStack User Guide Place two 1 4 inch dowel pins in the dowel pin holes of your benchtop You do not need to use all three dowel pin positions Pick up the VStack and align the dowel pin holes in its base with the dowel pins of your benchtop Place the VStack on the benchtop making sure that the dowel pins fit properly into each hole Screw two M6 flathead screws into two of the screw holes making sure they screw completely into the base You do not need to use all screw positions Check that the VStack is firmly mounted to your benchtop by trying to gently rock it Chapter 2 Installation 17 VStack User Guide Connect
14. corners and is selected in the Plate Selection list box If the plate type does not appear in the Plate Selection list box add definitions for the plate in the labware editor For more information see Adding a Plate Type To the Labware Database on page 39 Click Apply Place the plate on the plate stage For information about plate orientation see Placing a Plate on page 30 5 In the Teachpoints section next to the Orientation Sensor value click Move Here to move the stage to the Orientation sensor position Orientation Sensor a0 6 Examine the sensor readings and refer to the flowchart in Overview of Setting Up Orientation Checking on page 47 for what to do next The sensor intensity is adjusted using the labware editor See the definition of this property in the Database and Security User Guide Also see the flowchart in Overview of Setting Up Orientation Checking on page 47 for when to adjust this property The Orientation sensor threshold is adjusted using the labware editor See the definition of this property in the Database and Security User Guide Also see the flowchart in Overview of Setting Up Orientation Checking on page 47 for when to adjust this property When you added a labware entry the plate specific orientation sensor Offset was set to zero Earlier you entered a default value calculated from plate measurements Changing the Default Orientation Sensor Value on page 49 If this value is no
15. identified on the network The server will run on Windows XP NT and 2000 Installation Note You may need additional administrator privileges on the Procedure controlling computer to install the DHCP server Please check with your network administrator if you think you may not have sufficient privileges To install and start the DHCP Server service 1 Create the following directory on the controlling computer C Program Files Velocity1 1 DHCP server 2 Extract the files from the DHCP Server CD ROM into this new folder Double click the file named dhcpsrv exe The DHCP Server window opens DHCP Server x Do you want to install the DHCP server as a Windows NT 2000 service Q Yes No Don t ask this question again 4 Click Yes This installs the DHCP server as a service on your computer The DHCP Server window opens and displays the status as Running Stopping and Starting the Service Chapter 2 Installation ed VStack User Guide DHCP Server r Service Welcome to the DHCP Server v1 5 2 Install Hemove al Start Stop Written by Uwe Ruttkamp Status Running 5 Click the close box to close the window The DHCP Server service will now automatically run every time you turn on the controlling computer You can stop and start the DHCP Server service from within Windows by navigating to Start Programs Administrative Tools Services Consult y
16. of a rack that is mounted on a device Figure Door mechanism on the front load rack Door Door Thumb tab Door release button Stacker gripper hidden To load labware in a front load rack IMPORTANT Before you attempt to load the labware in a mounted rack ensure the device is ready for loading For example the clamps in the BenchCel stacker head must be closed extended See your device user guide for details 1 On each side of the rack slide the Door release black buttons forward while pushing outward on the thumb tabs The rack doors open Figure Opening the front load rack 2 Place the labware directly through the open rack doors so that the bottom labware rests on the rack stacker grippers Ensure the labware is level and securely in the rack 3 To close the doors press the thumb tabs inward until the doors snap shut To unload labware from a front load rack IMPORTANT Before you attempt to unload the labware from a mounted rack ensure the device is ready for unloading For details see your device user guide 1 To open the rack doors slide the black Door release buttons forward on each side of the rack while pressing outward on the thumb tabs 2 Carefully lift the labware out through the front of the rack Accessing product user documentation Velocityll product user documentation is available in the following formats Online help available within the software PD
17. presence of a notch and a sensor value above the threshold indicates the absence of a notch The default threshold is 0 units which must be changed for correct orientation checking For more information see the Database and Security User Guide 46 Chapter 3 Configuration and Manual Operation VStack User Guide You may need to adjust one or more of these settings to optimize orientation checking accuracy IMPORTANT The Orientation sensor teachpoint also affects orientation checking but does not change its value Overview of Setting Up Orientation Checking Turn on orientation checking Y Change the default Orientation sensor offset Y Place plate on stage and examine sensor readings The overall process of setting up orientation checking is shown in the following diagram Upstack and downstack a few plates to test Repeat for each type of plate on each VStack Setthe Orientation lt sensor threshold e Is difference between left and right notch sensors gt 100 Are sensors both at their top limit Yes Adjust the No about 230 or bottom limit 0 Adjust the Orientation sensor offset Does that create a difference of Sensor intensity Use this diagram in combination with the following procedures to set up orient
18. which is bonded to conductive parts of an equipment for safety purposes Indicates that you must not discard this electrical electronic product in domestic household waste Chapter 1 Introduction Safety and regulatory compliance The VStack complies with the applicable EU Directives See the Declaration of Conformity or Declaration of Incorporation as applicable for details The Labware Stacker is designed to comply with the standards listed in the following table Regulatory Compliance Standard EMC European Union EMC Directive 2004 108 EC IEC 61326 1 2005 EN 61326 1 2006 Canada ICES NMB 001 2004 Australia New Zealand AS NZS CISPR 11 2004 Safety European Union Machinery Directive 2006 42 EC Low Voltage Directive 2006 95 EC IEC 61010 1 2001 EN61010 1 2001 Canada CAN CSA C22 2 No 61010 1 04 USA ANSI UL 61010 1 2004 Electromagnetic compatibility If the VStack causes interference with radio or television reception which can be determined by turning the device off and on try one or more of the following measures e Relocate the radio or television antenna Move the device away from the radio or television Plugthe device into a different electrical outlet so that the device and the radio or television are on separate electrical circuits Make sure that all peripheral devices are also certified Make sure that appropriate cables are used to connect the device to peripheral equip
19. 1 8 inch tubing If your house air uses a threaded nozzle attach the 1 8 inch NPT fitting to your house air 3 Push the other end of the 1 8 inch tubing into the air input port on the back of the VStack 4 Tug gently on the air line If you feel resistance the line is in place 5 Turn on the air and make sure that the air pressure does not exceed 120 psi The air pressure at the gripper is internally regulated to 40 psi although it may be changed for particular plate types For more information about adjusting the pressure see Adjusting the Grip Pressure on page 41 a Chapter 2 Installation VStack User Guide About VStack Networking Communications Introduction DHCP Server Kit About IP Addresses Automatically Assigned IP Addresses A VStack receives its operational commands from a computer to which it is networked The network used for communication is a standard Ethernet network consisting of a network card in the controlling computer and an Ethernet cable between the computer and VStack If you want to have more than one VStack in your system the network must include an Ethernet switch to serve as an adapter between the single Ethernet cable attached to your computer and the Ethernet cables of the multiple VStacks INJURY HAZARD Run your VStacks on a dedicated lab automation network and not a general organizational network It is unsafe to allow control of VStacks from computers on a general net
20. 19 VStack User Guide When the DHCP server starts it reads an initialization file that points the server to the network card through which the computer will serve addresses When you install the network card you must assign the IP address and subnet mask so that it matches the information in the initialization file To set up a network for your VStack or VStacks perform the following procedures Step Procedure 1 Installing the Network Card on page 20 2 3 4 5 6 Installing the DHCP Server on page 22 Connecting the VStack To the Network on page 24 Turning On the VStack on page 25 Installing Software on page 25 Verifying Network Communication on page 31 Chapter 2 Installation VStack User Guide Installing the Network Card Introduction This section assumes that you have purchased the DHCP Server Kit from Velocity11 The network card supplied with your DHCP Server Kit must be installed for your VStack to be able to communicate with the controlling computer assuming that you want to use your existing network card to communicate with your organization s network Installation Note You may need additional administrator privileges on the Procedure controlling computer to install the network card Please check with your network administrator if you think you may not have sufficient privileges To install and configure the network card 1 Install a new network interface card a Locate the PCI Adapter
21. 3 In Motion Plate Present Busy BIN Notch Right Notch Red Plate Absent r Gripper Delay jl XX r Teach Points r VFD Display Orientation Sensor 80 00 ISP m Plate Selection Presentation 2 200 TeachHere Move Here 384 Auto wash Reservoir Normal Lidded Sealed Orientation Checking r Stacker Selection 5 CENE 11 OK Apply 06 Plate specific grip offset 0 Plate specific orientation sensor offset Chapter 3 Configuration and Manual Operation VStack User Guide Selecting a Plate Type Introduction Procedure What To Do Next For the VStack to correctly handle a plate during manual real time operation you must select the type of plate you intend to use from the labware database If the type of plate you intend to use is not already defined in the labware database you will need to create an entry for the plate and then configure the entry s properties before returning to this section and selecting the plate type For more information see Adding a Plate Type To the Labware Database on page 39 To select a plate for a manual operation l Open the VStackBioNet Diagnostics window For directions see Opening Diagnostics for a Specific VStack on page 36 2 Inthe Plate Selection group box of the Diagnostics page select a plate from the list box r Plate Selection
22. 57 Chapter 4 Maintenance and Troubleshooting VStack Error lt 60 Maintenance 5 66 Chapter 5 ActiveX Commands About VStack ActiveX 69 Introduction This chapter introduces the VStack lists the system requirements gives general safety information and provides an important overview of the product Chapter 1 Introduction VStack User Guide About this Guide Intended Audience This guide is intended to be read by Everyone who uses the VStack Q Lab personnel who install and configure the VStack Q Developers who integrate the VStack into third party systems VStack Software This version of the VStack User Guide guide is only for use with VStack Version ActiveX software version 4 0 You can find the version number after installation To find the version number of your VStack ActiveX software 1 In your Windows operating system search for the file named VStackBioNet ocx Right click the file and select Properties Click the Version tab The version number is displayed You only need to consider the first two parts of the version number VStackBioNet ocx Properties 21 x General Version Security Summary File version Description VStackBioNet ActiveX Control Module Copyright Copyright 2001 2003 M Other version information Item name
23. F files on the software CD Printed books You can also search the online help or download the latest version of any PDF file from the Velocityll website at http www velocityll com support knowledge base Contacting Velocity11 Technical Support 1 800 979 4811 or 1 650 846 6611 Customer Service 1 866 428 9811 or 1 650 846 6601 Email infoQvelocityll com Web http www velocityl 1 com Velocity11 an Agilent Technologies Company 3565 Haven Avenue Menlo Park CA 94025 USA 1 650 846 6600 Contents Chapter 1 Introduction About this OUIde saxo ues dub potens Peete E EP n reb ER EA 2 Computer and Laboratory Requirements 4 General VStack Hazard Warnings 5 VStdoKcOVelVIBW xo NEPOS EU cn SEU AL EE UR UR 7 6 Chapter 2 Installation Unpacking The Voldol eet Ma mI LE 10 VS tack PANS Me aod od A Bate MEd 12 MOUNTING the 15 Connecting Power and Air 17 About VStack Networking Communications 18 Installing the Network Card 20 Installigthie DROPCSeRUBEA 2 to eee race Bi ea oh 22 Connecting the VStack To the Network 24 Turning On the 5
24. Labware Stacker and Rack Handling User Guide Original Instructions GE Agilent Technologies Notices Agilent Technologies Inc 2011 No part of this manual may be reproduced in any form or by any means including electronic storage and retrieval or translation into a foreign language without prior agreement and written consent from Agilent Technologies Inc as governed by United States and international copyright laws User Guide Part Number G5407 90001 Edition Revision 01 December 2011 Updates title page Safety Notices compliance declarations general safety warnings and ActiveX Revision 00 November 2003 Contact Information Agilent Technologies Inc Automation Solutions 5301 Stevens Creek Blvd Santa Clara CA 95051 USA Technical Support 1 800 979 4811 or 1 408 345 8011 service automation agilent com Customer Service 1 866 428 9811 or 1 408 345 8356 orders automation agilent com European Service 44 0 1763208826 euroservice automation agilent com Documentation feedback documentation automation agilent com Web www agilent com lifesciences automation Acknowledgements Microsoft and Windows are either registered trademarks or trademarks of the Microsoft Corporation in the United States and other countries Warranty The material contained in this docu ment is provided as is and is sub jectto being changed without notice in future editions Further to the ma
25. NT See your BenchCel User Guide or VStack User Guide for the details on how to release a rack for removal or to prepare for loading a mounted rack To load labware into a standard or top load rack 1 If possible remove the rack from the device and place the rack on a flat level surface 2 Using both hands carefully slide a small stack of labware down through the top of the rack You can use one hand to support underneath the labware stack while the other hand holds the top of the labware to keep it level See the following figure WARNING Use care to avoid sliding your hand on the interior edges in the rack The edges can have sharp surfaces Figure Loading a standard rack Interior edges can be sharp 3 Standard racks only When you reach the bottom of the open slot transfer your hand positions so that you continue supporting the labware through the bottom slot Figure Supporting labware through the bottom slot Stacker grippers on tab interior hidden 4 Ensure that the bottom labware in the stack rests on the rack stacker grippers To unload labware from a standard or top load rack With your hands positioned as shown in the previous figures carefully slide the labware in small stacks up and out of the top of the rack Loading labware in a front load rack The doors on the front load rack provide easy access for loading labware into the front
26. and Manual Operation VStack User Guide Sensor Rack Present Plate Present Stage Position Description A white number indicates that a rack is loaded on the VStack orange number of less than 20 indicates that there is no rack loaded on the VStack white number indicates that a plate is in the VStack grippers orange number indicates that no plate is present in the grippers This sensor is the large unlabeled value It indicates the plate stage position in relation to the home position at the bottom The measurements are in millimeters with positive values meaning above the home position Chapter 3 Configuration and Manual Operation 45 VStack User Guide Configuring Plate Orientation Checking About Orientation Sensing Control Orientation checking check box Plate specific orientation sensor offset Sensor intensity Orientation Sensor threshold The VStack has two sensors that sense the presence of a notch in the left or right far corners of a plate from the robot s perspective Although there are only two notch sensors the software is aware of the existence of four plate corners so that if a plate with two notches is on the stage and no notches are detected you will receive an error message telling you to turn the plate around 180 The controls that affect orientation checking are listed in the following table The default values will not support accurate orientation check
27. anual Operation 49 VStack User Guide The default setting for the plate specific orientation sensor offset and therefore also the labware editors orientation sensor offset is zero You must change this value for orientation sensing to work You can calculate an initial value by taking the average of the height of the plate plus the height of the plate s skirt 2 where x and y are dimensions shown in the following diagram n i 00023 This value may not need to be further adjusted To enter the initial plate specific orientation sensor offset value 1 Open the VStack Diagnostics software For more information see Opening Diagnostics for a Specific VStack on page 36 2 Enter the value for the Plate specific orientation offset Click Apply to save the changes 50 Chapter 3 Configuration and Manual Operation VStack User Guide Placing a Plate and Examining Sensor Readings Adjusting the Sensor Intensity Adjusting the Orientation Sensor Threshold Adjusting the Orientation Sensor Offset Before making further adjustments to sensor settings place the plate you want to use on the VStack and examine the current readings To examine the sensor readings 1 Open the VStackBioNet Diagnostics software For directions see Opening Diagnostics for a Specific VStack on page 36 2 Make sure that the plate you plan to use has only one notch positioned in either of the far
28. ation checking gt 100 Chapter 3 Configuration and Manual Operation VStack User Guide Yes 47 48 Chapter 3 Configuration and Manual Operation VStack User Guide Turning On Orientation Checking DODDO Turning On Orientation Checking on page 48 Changing the Default Orientation Sensor Value on page 49 Placing a Plate and Examining Sensor Readings on page 50 Adjusting the Sensor Intensity on page 50 Adjusting the Orientation Sensor Threshold on page 50 Adjusting the Orientation Sensor Offset on page 50 The overall goal is to obtain Left Notch and Right Notch sensor values that are widely separated and an Orientation sensor threshold that lies roughly half way between those values Note If orientation checking is turned off the plate stage still moves to the Orientation teachpoint during upstack and downstack operations but the orientation is registered as correct regardless of the actual orientation of the plate To turn on plate orientation checking 1 Open the VStack Diagnostics software For more information see Opening Diagnostics for a Specific VStack on page 36 In the Plate Selection group box select the Orientation Checking check box r Plate Selection 384 Auto wash Reservoir E Normal Lidded Sealed Orientation Checking Click Apply to save the changes Changing the Default Orientation Sensor Value Chapter 3 Configuration and M
29. bleshooting information given in Maintenance and Troubleshooting on page 59 Modify the grip pressure as described in Adjusting the Grip Pressure on page 41 To downstack a plate I oS Open VStackBioNet Diagnostics For directions see Opening Diagnostics for a Specific VStack on page 36 Select your plate type from the list box and click Apply Load a rack containing plates Make sure that your plates are properly positioned in the rack For more information see Placing a Plate on page 30 Click Release Stack to lock the rack into place Click Downstack Remove the plate from the stage Click Downstack and repeat until you are satisfied that the downstacking operation is working correctly To upstack a plate 1 Place a plate on the stage Click Upstack Repeat until you are satisfied that the upstacking operation is working correctly Chapter 3 Configuration and Manual Operation i VStack User Guide Adjusting the Grip Pressure Definition When To Adjust the Grip Pressure Grip Pressure Adjustment The grip pressure determines the force with which the VStack grips the plates It is a property of a specific VStack and not the type of plate You can see the sensor reading for grip pressure in the VStackBioNet Diagnostics window For more information about sensor readings see Viewing Sensor Readings on page 43 If the plates you are using are flimsy they may distor
30. ckBioNet 2 Click Display Diagnostics 3 Click in the Status column to reveal a drop down list 4 From the list box select Delete Status New Delete Note Other commands in this list box are for service use only Configuration and Manual Operation This chapter shows you how to set up a VStack and perform manual real time operations If your lab automation system is already set up and you only need to check that a particular plate works with a particular stacker follow the procedures in this table Step Procedure 1 Opening Diagnostics for a Specific VStack page 36 2 Selecting a Plate Type on page 38 3 Testing Plate Handling on page 40 If you have just installed the VStack you will need to completely configure it using the procedures in the above table as well one or more of the following procedures depending on your situation Adding a Plate Type To the Labware Database on page 39 Adjusting the Grip Pressure on page 41 Adjusting the Plate Specific Grip Offset on page 42 Configuring Plate Orientation Checking on page 45 DODDO Displaying a Message on page 54 Changing the Presentation Teachpoint on page 56 In addition you may need to perform some procedures given in the Database and Security User Guide This guide will direct you to those procedures at the appropriate places After your VStack is set up and routinely running protocols you may occasi
31. d a rack by the interior edges The interior edges can have sharp surfaces that can cause cuts if handled improperly CAUTION A rack that is fully loaded with labware can be heavy Grasp the rack handle firmly to prevent the rack from slipping or tilting To carry a rack Firmly grasp the rack by the handle Figure Carrying a front load rack Handle Interior edges can be sharp Lifting the racks Make sure to use the proper lifting technique when mounting a rack on a device or removing a rack from a device WARNING Avoid touching the interior edges of a rack when lifting the rack The interior edges can have sharp surfaces IMPORTANT See your BenchCel User Guide or VStack User Guide for the procedure to mount a rack on the device or to release a rack from the device To lift a rack Use both hands to grasp the rack securely around the four corners near the base as the following figure shows Figure Lifting a standard rack Interior edges can be sharp Loading labware into the racks Before loading the labware into a rack Position the rack so that the opening is facing you Determine how the microplates should be oriented in the rack For example if the BenchCel orientation sensing feature is enabled make sure the Al wells are oriented in the rack as specified Loading labware in standard and top load racks IMPORTA
32. e stage The stage moves when you turn on the VStack Damage hazards Safety labels Chapter 1 Introduction DAMAGE HAZARD Always make sure that the plate stage is empty before you start to load or release a rack DAMAGE HAZARD Do not use harsh abrasives corrosive cleaning agents or metal brushes to clean VStack components or accessories Do not use any concentration of household bleach or sodium hypoclorite on the VStack Do not allow cleaning agents to contact electrical or sensitive mechanical components Warnings in the user documentation or on the device must be observed during all phases of operation service and repair of this device Failure to comply with these precautions violates safety standards of design and the intended use of the product Agilent Technologies assumes no liability for the customer s failure to comply with these requirements The following table lists the common symbols you might find on the device The symbol on the label indicates the risk of danger A description of the warning and information that will help you avoid the safety hazard are provided in this guide Description Indicates that you must read the accompanying instructions for example the safety guide for more information before proceeding Indicates hazardous voltages Indicates pinch crush and cut hazard Indicates laser hazard Indicates hot surface hazard Indicates protective conductor terminal
33. edure To perform a manual operation 1 Open the VStackBioNetDiagnostics software For more information see Opening Diagnostics for a Specific VStack on page 36 2 Click one of the following buttons Command Description Toggle Gripper Opens or closes the grippers Do not use the Gripper Delay slider without proper instruction from a Velocityll representative The default value is 250 This value synchronizes the closing of the gripper with the movement of the plate stage and you should not have to change it Operations Group Box Downstack Transfers plates from the rack to the plate stage Upstack Transfers plates from the plate stage to the rack Release Stack Allows you to lock or unlock the rack from the base Home Moves the plate stage to the home position and then the presentation position Jog Controls Group Box Up Moves the plate stage up by the distance in millimeters set in the adjacent slider control Down Moves the plate stage down by the distance in millimeters set in the adjacent slider control The In Motion light indicates that the plate stage is moving The Busy light indicates that the VStack is performing an operation and cannot perform another operation until the current one is complete Chapter 3 Configuration and Manual Operation VStack User Guide 53 Chapter 3 Configuration and Manual Operation VStack U
34. ent Possible Cause The rack is not properly positioned in the VStack or there is no rack The Rack Present sensor has failed Solution Check that the rack is positioned correctly in the rack grippers and click OK For more information see Loading and Releasing a Rack on page 28 In the VStack Diagnostics window make sure that the Rack Sensor reads a value greater than 20 when a rack is loaded If it does not the sensor has failed The VStack stage failed to move to the commanded position x Stage position error Possible Cause Something is obstructing the VStack stage and preventing it from reaching its commanded location The stacking thickness is not set correctly in the labware editor The plate is not sitting properly on the plate stage A mechanical or communications failure has occurred Solution Check for debris that may be blocking the VStack stage Measure the thickness of the plate and make sure that the correct value is entered For more information see the Database and Security User Guide Reset the plate and click OK Click OK and see if the VStack will recover Chapter 4 Maintenance and Troubleshooting 66 VStack User Guide Maintenance Schedule Schedule Maintenance tasks for the VStack are listed in the following table Maintenance Task Time Schedule Symptoms Wipe grippers with Ethanol As needed Grippers appear dirty or are sticking to plates Replace
35. entifies a VStack on the network You can change the Device Id to an easily recognizable name or number such as the VStack s serial number or a simple identifying number that you have applied to the outside of the VStack Note Device Ids are specific to a computer If you change a Device Id the change will not be transferred to other computers used to control the VStacks on the network You must change the Device Id on each computer To change the Device Id 1 Navigate to Start gt Programs gt Velocity11 and select VStackBioNet The VStackBioNet Testcontainer software opens E x Display Diagnostics Cycle Test Downstacks 0 Upstacks 0 Releases 2 Click Display Diagnostics 3 Click to select the VStack from the list 4 Click the number in the Device Id column The cursor appears 5 in the new Device Id Device Id Device Type IP Address MAC Address Status 500000 VStack 10 10 10 173 00 90 2 0 33 2 Matched Chapter 2 Installation VStack User Guide Deleting Old VStacks Introduction If you remove a VStack from the network it will still show up in the Discovered BioNet Devices window see Identifying VStacks on page 32 The entry will be reused if you add the VStack back later If you do not plan to add the VStack back into the network you may want to delete the entry Procedure To delete an unused device 1 Navigate to Start gt Programs gt Velocity11 and select VSta
36. er Guide 10 Repeat step 7 to step 9 to change this value until the VStack grippers hold the labware at the position you want Viewing Sensor Readings Procedure To view sensor readings 1 Open the VStack Diagnostics software For more information see Opening Diagnostics for a Specific VStack on page 36 2 View the sensor display Grip Pressure rT IPSI m Rack Present Left Notch Right Notch Red Plate Absent Sensor Types The following sensors are used on the VStack Sensor Description Left Notch Detects the presence of a notch in the left corner of the plate when the plate stage is at the Orientation Sensor teachpoint white number indicates the absence of a notch orange number indicates the presence of a notch For more information about the Orientation Sensor teachpoint see About Teachpoints on page 54 Right Notch Detects the presence of a notch in the right corner of the plate when the plate stage is at the Orientation Sensor or Grip teachpoint white number indicates the absence of a notch An orange number indicates the presence of a notch Grip Pressure Indicates the grip pressure when the grippers are closed in pounds per square inch positive number indicates that the grippers are closed When the grippers are open the value always reads zero For more information see Adjusting the Grip Pressure on page 41 44 Chapter 3 Configuration
37. eter The following fittings l 4inch quick connect 1 4 inch NPT l 4inch quick connect 1 8 amp inch NPT l 4inch quick connect 1 amp inch quick connect 1 4 inch quick connect T 1 8inch quick connect T Q An ActiveX CD ROM The VStack User Guide Getting Started Q The Database and Security User Guide European VStack Package Chapter 2 Installation 5 VStack User Guide The European VStack package contains 2 DODO A VStack and rack A country specific power cord An Ethernet cable 8 feet Polyurethane tubing 3 meters 6 mm diameter Polyurethane tubing 3 meters 4 mm diameter Polyurethane tubing 3 meters 1 8 inch diameter The following fittings 6 mm quick connect 4 mm quick connect 4 mm quick connect 1 8 inch quick connect 6 mm quick connect T 4 mm quick connect T 9999 1 8 inch quick connect T An ActiveX CD ROM The VStack User Guide Getting Started The Database and Security User Guide Chapter 2 Installation VStack User Guide VStack Parts Main Parts Diagram The main parts of the VStack are shown in the following diagram It will help you to be familiar with these terms as you read this guide i Rack Gripper Display Air pressure regulator Load Release button Plate stage Main Parts Description Part Air pressure regulator
38. gripper pads As needed Gripper pads are damaged or worn If you have any questions about maintenance please call the Velocity11 Service Center ActiveX Commands cthe ActiveX I ipused to operate the VStack when integrated with a third party systemJgbbidebrPd hboActiveX RpoaDr fab Chapter 5 ActiveX Commands VStack User Guide This page is intentionally left blank Index A ActiveX 7 events 73 finding the version 2 methods 70 properties 68 air connecting to 77 default pressure 17 input port 14 maximum pressure 17 operating pressure 4 air pressure regulator 13 B base 13 boolean Busy ActiveX property 69 boolean CheckPlateSensor ActiveX method 72 boolean GripperState ActiveX property 68 boolean IsRackLoaded ActiveX method 72 boolean IsRackPresent ActiveX method 72 boolean IsStageEmpty ActiveX method 71 boolean Open ActiveX method 70 boolean OpenlP ActiveX method 71 boolean OrientationChecking ActiveX property 69 boolean Vacuum ActiveX property 69 BSTR PlateName ActiveX property 68 Busy light 52 C communication verification 37 computer requirements 4 D damage hazards 5 Device Id about 33 changing 33 DHCP server installing 22 IP address 19 subnet mask 19 Diagnostics software 52 54 56 opening 36 display 13 documentation to read 3 dowel pins 15 Down command 52 Index 36 VStack User Guide Downstack command 52 down
39. ing so they must be correctly set for your VStack and plates Description Where Set Turns orientation checking on and off for both VStack BioNet manual operations and protocol runs using the Diagnostics VStack Note There is no relationship between this check box and the Check orientation check box in the labware editor which is used only for Velocity11 s BenchCel For more information see the Database and Security User Guide Adjusts the height of the plate stage for the selected Either plate type relative to the Orientation sensor VStack BioNet teachpoint Diagnostics L Labware editor Note This property is a proxy for the Orientation sensor offset in the labware editor When you change the value here the value of the Orientation sensor equivalent offset also changes The reverse is also true For property convenience you may decide to only adjust this value Orientation sensor in the labware editor offset Adjusting this value avoids having to adjust the actual Orientation Sensor teachpoint IMPORTANT Do not change the value of this teachpoint Adjusts the relative brightness of all sensor lamps Labware editor The default value is 50 For more information see the Database and Security User Guide Sets the Orientation sensor reading that defines the Labware editor boundary between the indicated presence and absence of a plate notch A sensor value below the threshold indicates the
40. ing To Power and Air Introduction Connecting the Power Cord Connecting the Air Line This section explains how to connect your VStack to the power and air supplies Connect the power cord first followed by the air line Review electrical requirements in Laboratory Requirements on page 4 before proceeding To connect the power cord 1 Plug one end of the power cord into the power port at the back of the VStack 2 Plug the other end of the power cord into an appropriately grounded electrical socket INJURY HAZARD Working with open charged air lines can result in injury if the end of the line whips around rapidly Switch the compressed air line off before installing the VStack If you have questions about setting up your air line contact your facilities department or the Velocity11 Customer Service Center DAMAGE HAZARD Make sure that air coming into the VStack is properly filtered for moisture and aerosol impurities there is no air filter on the VStack Dirt in unfiltered air can build up in air valves eventually causing a malfunction To connect the air line 1 Cut the supplied 1 8 inch tubing to the length you need for the air line Cut the tubing with a sharp craft knife and make sure that the edge of the tubing is square and clean 2 Plug one end of the 1 8 inch tubing into your lab s air supply You can also use 1 4 inch tubing from your lab s air supply with an adapter to switch to
41. irectly Automated Control When using the VStack during an automated protocol run it is controlled by platform software If you are using Velocityll s BioCel the platform software is VWorks If you are running the VStack in a system developed by your own organization the platform software will have been custom written using a program such as or Visual Basic Manual Control You need to control the VStack outside a protocol run to Q Recover from run errors C Test Ethernet communications Q Troubleshoot problems Set plate stage positions To do this Velocity1l provides software with a user interface called VStackBioNet Testcontainer software In addition Velocity11 provides a labware editor and database that you use to define labware dimensions The labware editor is accessed through the VStackBioNet Testcontainer software or VWorks Chapter 1 Introduction VStack User Guide Interaction of Software Components PC Labware editor Poir Labware database in Windows Registry TCP IP i ActiveX VStack commands commands VWorks firmware TCP IP ActiveX itchi commands ee Network including Diagnostics VStack commands commends Third party firmware protocol running software ActiveX commands VStackBioNet Testcontainer software ActiveX Software ActiveX is a set of technologies that allows software components to interact with each other rega
42. k and Presentation teachpoint For more information about the Presentation teachpoint see About Teachpoints on page 54 Stores microplates Can be removed from the VStack to add remove and transport plates 14 Chapter 2 Installation VStack User Guide VStack External Connections The VStack has the following external connections Quick connect air input port 1 8 inch Q Serial port Power receptacle Ethernet port These connections are shown in the following diagram 00024 Air input port Ethernet port Serial port On Off switch Power receptacle The serial port is used by Velocity11 to install firmware on the VStack Chapter 2 Installation 15 VStack User Guide Mounting the VStack About Mounting the VStack Procedure For the robot to move plates to and from the plate stage the three dimensional position of the plate stage must be fixed and known to the controlling software of the lab automation system This requires that the VStack is mounted on a stable base that does not move in relation to the robot You can mount the VStack directly on a benchtop or on a mounting plate that also holds other components of your system You should have the benchtop or mounting plate accurately drilled to create dowel pin holes and tapped to create screw threads according to the dimensions given in this section When you are planning where to install your VStack make sure you
43. leshooting VStack User Guide Plate on Stage Isthe The VStack sensed a type of plate that is different from the type of plate Wrong Type selected eo Plate on stage is the wrong type Possible Cause The incorrect plate was selected The notches are set incorrectly in the labware editor The plate is rotated 180 so that the notch is not in the specified position The orientation sensors are not configured properly One or both of the notch sensors has failed Solution In the Plate Selection group box select the plate type you are using Choose the correct plate in the Plate Selection menu Edit the notch location or locations of that plate For more information see the Database and Security User Guide Rotate the plate to the specified position and click OK For more information see the Database and Security User Guide Make sure that the sensor intensity and sensor threshold configurations are correct For more information see the Database and Security User Guide If the sensor values do not change with the presence or absence of a plate in the orientation sensor position one or more sensors may have failed Contact the Velocity11 Service Center for help Rack Not Present Stage Position Error 65 Chapter 4 Maintenance and Troubleshooting VStack User Guide The VStack failed to sense a rack when you clicked the Release Stack Upstack or Downstack buttons eo Rack not pres
44. ment Consult your equipment dealer Agilent Technologies or an experienced technician for assistance Changes or modifications not expressly approved by Agilent Technologies could void the user s authority to operate the equipment Sound emission declaration Sound pressure Lp lt 70 dB according to EN 27779 1991 Schalldruckpegel LP 70 dB nach EN 277779 1991 Chapter 1 Introduction VStack User Guide VStack Overview Product Description VStack Network Software Overview The VStack is a microplate stacker used in lab automation systems to dispense and receive plates It holds deepwell and shallow well plates pintools tipboxes and lids Typically several VStacks are used in a single lab automation system The VStack communicates with the lab automation system using an Ethernet network and DHCP server which dynamically recognizes VStacks as they are added to and removed from the system The VStack has few hardware controls it is controlled almost entirely by ActiveX commands issued by software installed on a computer You can interact with the VStack by Writing a protocol that is then run automatically on a lab automation platform according to a programmed schedule controlled by platform software Sending individual commands in real time directly to the VStack from software supplied with your VStack The VStack is intended to be routinely used for automated runs but you also need to control it d
45. ms gt Velocity11 and select VStackBioNet The VStackBioNet Testcontainer software opens 5 Display Diagnostics Cycle Test Downstacks 0 Upstacks 0 Releases Click Display Diagnostics Discovered BloNet Devices E Denes Id Denice IP Address Slatus 6 101010173 Malched VStack 00 90 02 3 Nat Found D Nat Found Not Found Nat Found Nol Found Nat Found Nol Found Mot Found Not Found Mot Found Nat Found Not Found 11 c Select the Elhamet adapler to use from the list below EtherLink XL 10 100 PCI TX NIC 3305B TX Packet Scheduler Minport wh IP 10 10 10 105 xl If the Discovered BioNet Devices window opens and the VStack has astatus of Matched you know that your computer is communicating with the VStack For more information about identifying VStacks on the network see Identifying VStacks on page 32 Click OK If you want to verify that you are communicating with a particular VStack click Move Here at the Orientation Sensor teachpoint of the VStackBioNet Diagnostics dialog box The plate stage will move to the Orientation teachpoint For more information see Opening Diagnostics for a Specific VStack on page 36s Chapter 2 Installation VStack User Guide Identifying VStacks Introduction Procedure A VStack s IP address may change as you turn the VStack on and off To allow you to select a
46. n 44 types 43 viewing readings 43 serial port 14 short Downstack ActiveX method 70 short Home ActiveX method 71 short Jog ActiveX method 71 short PlateDimensionSelect ActiveX property 68 short ReleaseStack ActiveX method 70 short Upstack ActiveX method 70 software Diagnostics 8 installing 25 overview 6 Stack Release teachpoint 54 Stage Position sensor 44 starting see VStack 25 switch 24 system requirements ments 4 T teachpoint about 54 Grip 54 Orientation Sensor 54 Presentation 55 changing 56 Stack Release 54 testing plates 40 Toggle Gripper command 52 U Up command 52 Upstack command 52 upstacking a plate 40 V void AboutBox ActiveX method 72 void Close ActiveX method 70 void DisplayMessage ActiveX method 71 void SetButtonMode ActiveX method 71 void ShowDiagsDialog ActiveX method 72 VStack basic description 6 external connections 14 identifying on network 32 33 manual operation 52 overview 6 parts description 13 parts diagram 12 parts inspection 70 turning on 25 unpacking 10 VStack error messages 60 VStackBioNet ocx see ActiveX 2 see computer require j Agilent Technologies User Guide G5407 90001 Revision 01 December 2011
47. ner with the notch has a white value This position indicates that the top of the plate is just aligned with the top of the sensors vertical range 6 Write down the value of the stage position the large number in the sensor display 7 Continue to click Up until both left and right notch values are large and white At this point the light beams on both sides are hitting the top of the plate skirt and reflecting back 8 Record the stage position value 9 Calculate the average of the two values you recorded This represents the mid point of the valid sensor range 10 Click Move Here for the Orientation Sensor teachpoint to move the plate stage to the teachpoint 11 Compare the stage position value of the teachpoint with the value that you calculated in step 9 The difference represents the Plate specific orientation offset you should apply to achieve an optimal reading position 12 Enter the value for the Plate specific orientation offset For example if the Orientation Sensor teachpoint stage position value is 65 mm and the calculated value is 70 mm enter an offset value of 5 mm 13 Click Apply to save the changes Chapter 3 Configuration and Manual Operation VStack User Guide Operating the VStack Manually Introduction Once your VStack is configured you can operate it manually using VStackBioNetDiagnostics software If you configured the VStack you will have already used some of these controls Proc
48. onally need to make minor adjustments using these same procedures Chapter 3 Configuration and Manual Operation VStack User Guide Opening Diagnostics for a Specific VStack Introduction Velocity11 provides the VStackBioNet Diagnostics software for configuration and manual control of the VStack Many configuration procedures first require you to open the VStackBioNet Diagnostics software for a specific VStack Procedure Note you are using the VStack on the BioCel refer to the BioCel User Guide for how to open the VStack Diagnostics software from within VWorks To open the VStack Diagnostics software 1 Navigate to Start gt Programs gt Velocity11 and select VStackBioNet This opens the VStackBioNet Testcontainer software x Display Diagnostics Cycle Test Downstacks 0 Upstacks 0 Releases 0 The Cycle Test button in the VStackBioNet Testcontainer dialog box is used during manufacturing to test the VStack It has no purpose during normal use 2 Click Display Diagnostics The Discovered BioNet Devices window appears For more information see Verifying Network Communication on page 31 Select your VStack from the list Click OK The VStackBioNet Diagnostics window opens Chapter 3 Configuration and Manual Operation d VStack User Guide StackBioNet Diagnostics cx8 Diagnostics Motor m Controls r Operations Grip Pressure IPSI Dom Upstack MESS a Release Stack
49. our Windows documentation for more information You can also start and stop the service using the executable To stop and start the DHCP Server service 1 Navigate to C Program Files Velocity1 1 DHCP server 2 Double click the file named dhcpsrv exe 3 Click Stop to stop the service and Start to start the service Chapter 2 Installation VStack User Guide Connecting the VStack To the Network Introduction Connecting a VStack To the Network Connecting Multiple VStacks This section assumes that you have purchased the DHCP Server Kit from Velocity11 To connect the VStack to the network you must connect the Ethernet switch to the PC Q VStack to the Ethernet switch To connect the Ethernet switch to the PC 1 Locate the 8 port Ethernet switch included in the DHCP Server Kit 2 Connect the included power cord to the switch 3 Locate the Ethernet cable included in the DHCP Server Kit 4 Plug one end of the cable into any of the eight ports in the Ethernet switch 5 Plug the other end into the new network card in the PC To connect the VStack to the Ethernet switch 1 Locate another Ethernet cable included with the Accessories 2 Plug one end of the cable into any of the eight ports on the Ethernet switch 3 Plug the other end into the Ethernet port of the VStack You can connect up to six more VStacks to the network using the 8 port switch Up to 25 VStacks can be used on this network if
50. particular VStack to communicate with there must be another way to identify a specific VStack The Device Id which does not change when the VStack is turned on and off is used for this purpose Device Ids are assigned in the order that VStacks are first added to the network You should either keep a record of which Device Id corresponds to which VStack or rename the Device Ids to values that are easily recognizable see Changing the Device Id on page 33 To identify the VStacks on your network 1 Turn off all VStacks at their on off switches 2 Turn on one VStack 3 Navigate to Start gt Programs gt Velocity11 and select VStackBioNet 4 Click Display Diagnostics or more VStacks appear in the dialog box but only one has a Status of Matched Dewce ld Denice IP Address Address Status 6 VStack 101010173 00 30 2 0 33 22 Malched Cancel Select the Ethemet adapler to use from the list below 300 1 EtherLink XL 10 100 PC TX NIC 32305B TX Packet Schedule Minipot wih IP 1011010105 5 5 Note the Device Id of the VStack 6 Turn on another VStack click Cancel in the Discovered BioNet Devices dialog box and click Display Diagnostics again Note the Device Id of the new VStack 7T Repeatstep 6 on other VStacks in turn recording their Device Ids Chapter 2 Installation 33 VStack User Guide Changing the Device Id Introduction Procedure The Device Id id
51. ration without first loading rack Possible Cause Solution The rack is not properly positioned Check that the rack is positioned in the VStack correctly in the rack grippers and click OK For more information see Loading and Releasing a Rack on page 28 The Rack Present sensor has In the VStack Diagnostics window failed make sure that the Rack Present sensor reads a value greater than 20 when a rack is loaded For more information see Opening Diagnostics for a Specific VStack on page 36 If the sensor reads a value of less than 20 and the rack is properly loaded the sensor has malfunctioned Contact the Velocity11 Service Center for help 61 Chapter 4 Maintenance and Troubleshooting VStack User Guide Could Not Perform The VStack sensed a plate on the stage when you clicked the Release Operation With Plate Stack or Downstack button on Stage VStack Error x X Could not perform operation with plate on stage Possible Cause There is a plate on the stage The sensor threshold is set too low One or both of the notch sensors has failed Solution Remove the plate and click OK In the labware editor set the sensor threshold to the appropriate value for that plate type and attempt the operation again For more information see the Database and Security User Guide In the VStack Diagnostics window both notch sensors should read values close to zero when there is nothing in f
52. rdless of the language in which the components were created Using the VStack ActiveX simple commands can be used to initiate complex operations in networked VStacks The VStack s ActiveX software includes CL VStack Diagnostics software Q Methods which control individual operations Q Properties which set the values used in methods such as speed fast Q Events which are notifications that methods are complete or have errors To allow you to develop your own controlling software the chapter called ActiveX Commands on page 67 provides the ActiveX methods events and properties Chapter 1 Introduction VStack User Guide VStack Diagnostics Labware Database VStack Diagnostics is a window that is part of the ActiveX software From the VStack Diagnostics window you can Q Perform simple VStack operations such as Downstack Upstack and Release Stack Enter properties such as the plate specific grip offset the type of plate to use and the text to show on the VStack display View sensor readings Access the labware editor Set teachpoint offsets which provide positional information to the robot The labware editor is used to enter the dimensions of each type of plate used with the VStack This information required for proper plate handling is stored in the labware database which is maintained in the Windows registry The same labware database is also used with other Velocity11 products For
53. re plates Load the rack Follow the directions in Configuration and Manual Operation on page 35 to configure the VStack Contact the Velocity11 Service Center for help 63 Chapter 4 Maintenance and Troubleshooting VStack User Guide Plate Is Rotated The VStack sensed a notch in the incorrect position 180 eo Plate is rotated 180 Possible Cause The plate is rotated by 180 so that the notch is not in the specified position The notches are set incorrectly in the labware editor The orientation sensors are not configured properly One or both of the notch sensors has failed Solution Rotate the plate to the specified position and click OK For more information see the Database and Security User Guide In the labware editor make sure that the correct notch location is checked then make sure that the plate is oriented correctly Note If the problem persists and the run must continue turn off orientation checking For more information see Selecting a Plate Type on page 38 Make sure that the sensor intensity and sensor threshold configurations are correct For more information see the Database and Security User Guide If the sensor values do not change with the presence or absence of a plate in the orientation sensor position then one or more sensors may have failed Please contact Velocityll s Service Center for assistance 64 Chapter 4 Maintenance and Troub
54. ront of them If not the sensor has malfunctioned Contact the Velocity11 Service Center for help For more information see Opening Diagnostics for a Specific VStack on page 36 No Plate on Stage No Plate Available Chapter 4 Maintenance and Troubleshooting VStack User Guide The VStack failed to sense a plate when you clicked the Upstack button Stack Error x No plate on stage Possible Cause There is no plate available to upstack The sensor threshold is set too high One or both of the notch sensors has failed Solution Place a plate on the stage click OK and attempt the command again Adjust the sensor threshold value For more information see the Database and Security User Guide If the sensor intensity is set correctly and the notch sensor values do not change when a plate is on the stage in the orientation sensor position one or both of the notch sensors have failed Contact the Velocity11 Service Center for help The VStack failed to sense a plate when you clicked the Downstack button Possible Cause rack is present loaded or unloaded but it contains no more plates A rack is present and it contains plates but it is not loaded at the time you press the Downstack button The VStack is not correctly configured For example you are using a black plate and the sensor intensity is set too low The plate present sensor has failed Solution Add mo
55. ser Guide Displaying a Message Introduction Procedure The VStack display shows text messages Long messages are scrolled across the display You can display your own message on the VStack display To display a message 1 Open the VStack Diagnostics software For more information see Opening Diagnostics for a Specific VStack on page 36 2 Type your message in the Display text box Click Set About Teachpoints Teachpoints Defined A teachpoint is a position to which the plate stage moves when commanded As the plate stage only moves in a vertical plane teachpoints are defined by a single number which is the height of the stage above the home position in millimeters The home position lies at the bottom of the stage s range of movement and has a positional value of 0 mm Teachpoints are specific to a particular VStack Teachpoint There are four teachpoints described in the following table Locations Teachpoint Definition Default Value mm Stack Release The height at which the plate stage releases the 100 00 a IMPORTANT Do not change this value Grip The height at which the grippers grip the plate 84 00 during an upstack or downstack operation 1 IMPORTANT Do not change this value for VStacks used on the BioCel Orientation The height at which the VStack senses orientation 68 00 Sensor notches on a plate 1 IMPORTANT Do not change this value for VStacks used on the BioCel Chapter 3
56. stacking a plate 40 E error messages 60 Ethernet port 14 Ethernet switch 24 events ActiveX 73 G grip pressure 41 Grip Pressure sensor 43 Grip teachpoint 54 gripper location 73 hazards see safety Home command 52 In Motion light 52 IP address 78 IPictureDisp ControlPicture ActiveX property 68 J jog controls 52 L laboratory requirements 4 labware database 8 labware editor opening 39 Left Notch sensor 43 load release button 13 loading a rack 28 long SensorFrequency ActiveX property 68 longGripDelay ActiveXProperty 68 maintenance schedule 66 manual operation 52 Motor tabbed page 57 mounting the VStack 15 network connecting VStacks 24 identifying VStacks 32 34 verifying communication 37 network card installation 20 VStack User Guide networking kit 78 Orientation Sensor teachpoint 54 P Plate Present sensor 44 plate stage 13 plate testing 40 plate type selection 38 power connecting to 77 operating voltage and current 4 receptacle 14 Presentation teachpoint 55 changing 56 preventive maintenance 66 R rack 13 loading and releasing 28 Rack Present sensor 44 Release Stack command 52 releasing a rack 28 Right Notch sensor 43 S safety general hazards 5 injury hazards 5 screws 15 selecting a plate type 38 sensor Grip Pressure 43 Left Notch 43 Plate Present 44 Rack Present 44 Right Notch 43 Stage Positio
57. t accurate enough for your specific plate type and VStack you will have been directed here from the overview flowchart Follow this procedure to obtain a more accurate offset value Note that this property can be changed in either of two places The plate specific orientation sensor offset is a proxy of the labware editors Chapter 3 Configuration and Manual Operation 21 VStack User Guide orientation sensor offset When you make a change in one place the change is automatically copied to the other place The procedure in this section explains how to change the plate specific orientation sensor offset For more information about the orientation sensor offset see the Database and Security User Guide To adjust the plate specific orientation sensor offset 1 Remove the rack from the VStack For more information see Loading and Releasing a Rack on page 28 2 Place a plate that has a single notch on the plate stage For more information see Placing a Plate on page 30 In the VStackBioNet Diagnostics dialog box click Move Here for the Orientation sensor teachpoint The plate stage moves to the Orientation sensor position 4 Inthe Jog Controls group box set the slider to a value of one 1 mm and click Down until the left and right sensor readings are both zero At this point the light beam shines above the plate The Rack Present sensor should also read zero 5 Click Up until the sensor corresponding to the cor
58. t when held with the default pressure of 40 psi so you should lower the grip pressure If the stack of plates you are using is heavy because there are many plates in the stack and perhaps because they are made of a heavy material and contain a lot of liquid the bottom plate may fall out of the gripper In this case increase the grip pressure The grip pressure however should not exceed the maximum pressure specified in Laboratory Requirements on page 4 To adjust the grip pressure 1 Locate the air pressure regulator For more information see VStack Parts on page 12 2 Using your fingers or a pair of pliers unscrew the locking ring by turning it counterclockwise 3 Adjust the grip pressure by turning the regulator clockwise to increase the pressure and counterclockwise to decrease the pressure DAMAGE HAZARD Do not set the grip pressure lower than 15 psi Toggle the grippers 2 3 times to allow the pressure to adjust 5 Lock the locking ring by turning it clockwise Chapter 3 Configuration and Manual Operation VStack User Guide Adjusting the Plate Specific Grip Offset Definition The VStackBioNet Diagnostics softwares Plate specific grip offset specifies the distance below the standard Grip teachpoint When To Adjust Adjust this value only if the VStack is not gripping the plates at the correct position The Plate Specific The Plate specific grip offset is a property of the plate and not a property
59. tacks of labware microplates tipboxes and tube racks that are processed during a protocol run Figure BenchCel Workstation with front load labware racks Rack Stacker head For a description of the BenchCel device or VStack device see the BenchCel User Guide or VStack User Guide The labware racks are available in three models standard rack top load rack and front load rack 22825 00 00 00 RackHandlingGuide June08 Copyright 2008 Velocity11 An Agilent Technologies Company All rights reserved Figure Three models of labware racks Standard Top load Front load the rack models have the following basic parts Carrying handle The standard rack has a polished top bar that can be used as a carrying handle The top load and front load racks have fold down carrying handles Tabs A pair of vertical tabs are located at the bottom sides of the rack The tabs insert into slots on the device when you mount the rack Stacker grippers A gripper is located on the interior bottom of each tab The pair of grippers hold a microplate during the labware loading unloading downstacking and upstacking processes A clamp in the device opens and closes the grippers Figure Standard rack containing labware Handle Tab A stacker gripper hidden is located on interior of each tab Labware Aecaciryi An Agilent Technologies Company Carrying the racks WARNING Do not hol
60. the U S Gov ernment will receive no greater than Restricted Rights as defined in FAR 52 227 19 c 1 2 June 1987 U S Government users will receive no greater than Limited Rights as defined in FAR 52 227 14 June1987 or DFAR 252 227 7015 b 2 November 1995 as applicable in any technical data Safety Notices AN A WARNING or INJURY HAZARD notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly performed or adhered to could result in personal injury or death Do not proceed beyond a WARNING notice until the indicated conditions are fully understood and met A CAUTION DAMAGE HAZARD notice denotes a hazard It calls attention to an operating procedure practice or the like that if not correctly performed or adhered to could result in damage to the product or loss of important data Do not proceed beyond CAUTION notice until the indicated conditions are fully understood and met Letter to our Customers Dear Customer e Agilent Technologies The Agilent Technologies acquisition of Velocityll resulted in the following changes e Creation of Agilent Technologies Automation Solutions formerly Velocityll e Renaming of some Velocityll products e New Customer Service and Technical Support contact information e New website address for product information Please make a note of the following changes as they impact this user guide
61. this reason the details of the labware database are given in a separate publication the Database and Security User Guide Installation This chapter explains how to install the VStack The installation process includes Step Procedure 1 Unpacking the VStack on page 10 2 Mounting the VStack on page 15 3 Connecting To Power and Air on page 17 4 Installing the Network Card on page 20 5 Installing the DHCP Server on page 22 6 Connecting the VStack To the Network on page 24 7 Turning On the VStack on page 25 8 Installing Software on page 25 9 Loading and Releasing a Rack on page 28 10 Verifying Network Communication on page 31 Chapter 2 Installation VStack User Guide Unpacking the VStack Parts Inspection As you unpack the VStack inspect all parts for possible shipping damage If anything is missing or appears to be damaged contact the Velocity11 Service Center as soon as possible IMPORTANT Save the carton and packing materials in case you ever need to ship the VStack Please note that Velocity11 is not responsible for damage if the VStack is incorrectly packaged and moved by someone other than a Velocityll employee North American The North American VStack package contains VStack Package AVStack and rack A Power cord An Ethernet cable 8 feet Polyurethane tubing 10 feet 1 4 inch diameter Polyurethane tubing 10 feet 1 8 inch diam
62. to use is not already defined start by following the procedure in this section Opening the Note If you are using the VStack on the BioCel refer to the BioCel User Labware Editor Guide for how to open the labware editor from within VWorks Then refer to the Database and Security User Guide for how to use the labware editor To open the labware editor 1 Open VStackBioNet Diagnostics window For directions see Opening Diagnostics for a Specific VStack on page 36 2 Inthe Plate Selection group box click Edit r Plate Selection 384 Auto wash Reservoir Normal Lidded Sealed Orientation Checking 3 Refer to the Database and Security User Guide for directions for adding a plate type The Database and Security User Guide will direct you back to this guide for some of the procedures you need to perform Chapter 3 Configuration and Manual Operation VStack User Guide Testing Plate Handling Introduction Downstacking Plates Upstacking Plates After selecting the plate type you want to use perform some downstacking and upstacking operations with the plate to make sure that the plate is handled correctly by the VStack If the VStack performs without error it is ready for use with an automated protocol If you receive errors a Check the labware editor parameters for the labware you are using For more information see the Database and Security User Guide Check the trou
63. up will install files Unless you specify a different location the installation program will install the ActiveX in a folder it creates which is C Program Files Velocity11 VStack If you want to choose a different destination folder a Click Browse b Navigate to and select the folder Chapter 2 Installation 27 VStack User Guide C Click Next The Select Program Folder dialog box opens InstallShield Wizard Select Program Folder Please select a program folder d Inthe Program Folders text box type a name for the shortcut you want to be created in the Start gt Programs menu or leave the default name 8 Click Next 9 The Setup Status screen opens 10 When the text InstallShield Wizard Complete opens click Finish Chapter 2 Installation VStack User Guide Loading and Releasing a Rack Loading and When you stack plates into a rack the bottom plate sits on the gripper Releasing Defined When you install a rack into a VStack the bottom plate drops controlled by the plate stage to be held on the side by the gripper The rack is also held so it will not rock This process is called loading When the plate stage moves the bottom plate up so it rests on the gripper the rack becomes free to move This process is called releasing Note The VStack will not operate until the rack is loaded INJURY HAZARD Keep far enough away from the VStack while it is in motion so that you do not catch
64. work because remote computer operators may accidentally initiate an operation that causes the plate stage to move unexpectedly when lab personnel are nearby If you do not have an existing network for your VStacks you will need to set one up even if you are only using one VStack Note The exception to this is if you are using one VStack and the controlling computer does not need to be connected to your organization s network In this case you can use the computers existing network card and a crossover cable to connect to the VStack To set up the network you can buy the DHCP Server Kit from Velocity11 which includes A networking card An Ethernet cable An Ethernet switch C DHCP server software IP addresses allow computers and other devices to be identified on a network so information can be specifically routed to them When setting up the VStack network there are two implementations of IP addresses to consider U The automatically assigned IP address of each VStack Q The manually assigned IP address of the network card on the controlling computer that interfaces with the VStack network Each VStack on a network must have a unique IP address to allow the controlling computer to specifically send commands to it IP addresses are assigned automatically by a DHCP server that you install on the controlling computer Manually Assigned IP Addresses Overview of Setting Up the Network Chapter 2 Installation
65. x imum extent permitted by applicable law Agilent disclaims all warranties either express or implied with regard to this manual and any information contained herein including but not limited to the implied warranties of merchantability and fitness for a par ticular purpose Agilent shall not be liable for errors or for incidental or consequential damages in connection with the furnishing use or perfor mance of this document or of any information contained herein Should Agilent and the user have a separate written agreement with warranty terms covering the material in this document that conflict with these terms the warranty terms in the sep arate agreement shall control Technology Licenses The hardware and or software described in this document are furnished under a license and may be used or copied only in accordance with the terms of such license Restricted Rights Legend If software is for use in the performance of a U S Government prime contract or sub contract Software is delivered and licensed as Commercial computer soft ware as defined in DFAR 252 227 7014 June 1995 or as a commercial item as defined in FAR 2 101 a or as Restricted computer software as defined in FAR 52 227 19 June 1987 or any equivalent agency regulation or contract clause Use duplication or disclosure of Software is subject to Agilent Technologies standard commercial license terms and non DOD Departments and Agencies of
66. you add more 8 port switches Chapter 2 Installation 25 VStack User Guide Turning On the VStack Introduction Turn on the VStack after setting up the network and before installing the ActiveX software Turning on the To turn on the VStack VStack 1 Toggle the power switch to the on position The motor moves to the home position and the VStack processor obtains an IP address The text on the display reads Finding Server Installing Software Introduction For general information about the software that controls the VStack see VStack Overview on page 6 Installation To install the ActiveX software Procedure l Insert the CD ROM labelled VStackBioNet ActiveX into your PC 2 In Windows navigate to the CD ROM drive and open it to view the files on the drive 3 Double click the Setup exe file The InstallShield Wizard starts InstallShield Wizard Welcome to the InstallShield Wizard for VStack The InstallShield Wizard will install Stack on your computer To continue click Next 4 Click Next The Customer Information dialog box opens _ Chapter 2 Installation VStack User Guide InstallShield Wizard Customer Information Please enter your information Delighted Customer Productivity Inc 5 Enter your name and your company s name 6 Click Next The Choose Destination Location dialog box opens InstallShield Wizard Choose Destination Location Select folder where Set
67. your hands clothing jewelry and so on in the mechanism Loading a Rack To load a rack 1 Place a rack on the base unit lowering it so that the rectangular tabs drop into the slots in the gripper unit The rack can be placed with the open corner at the front or back Chapter 2 Installation 29 VStack User Guide 2 Either Press the load release button on top of the base unit Click Release Stack in the VStackBioNet Diagnostics window see Opening Diagnostics for a Specific VStack on page 36 The VStack loads the rack Releasing a Rack To release a rack 1 Either Press the load release button on top of the base unit Click Release Stack in the VStackBioNet Diagnostics window DAMAGE HAZARD Always remove the rack from the base unit before attempting to move the VStack 2 Liftthe rack up and off the base Chapter 2 Installation VStack User Guide Placing a Plate Plate Orientation To place a plate on the plate stage 1 Place the plate so that the Al well is at the far left as viewed by the robot A1 Well Chapter 2 Installation 31 VStack User Guide Verifying Network Communication Procedure After installing the ActiveX software follow this procedure to verify that the computer and VStack are communicating correctly To verify that the Computer and VStack are communicating 1 Navigate to Start gt Progra

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