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1.                                                                             To connect to the net work  with out changing  use the 3 pole  neutral and ground plug     Leave the neutral binding post out of use     PROGRAMMING MODE    8 22    R ESPINAR S L  AEi 01    The microprocessor of this autoclave has 10 programs  numbers from PO to  P9  First 4  from PO to P3  are protected programs and they come programmed    from the factory with the usual parameters for sterilization     These pre set programs are     Prog  N     Temp     C    Press   Ster  time    Ster  Mode   Drying time   bar     minutes  minutes  RIEN psg     m Je    NT  121 1 1 30 Solids  ieee                                  A    This programs cannot be modified  in case of modification protect or unprotect more       numbers of programs  See the programs protected     To edit different sterilization programs   use the programs between p4 p9 both included     ALPHANUMERIC DISPLAY FUNCTIONS    P7 1216 020min        SOL 1 SEC  30min     The alphanumeric screen apart from showing the sterilization parameters  also shows a  warning  failure and other messages  See WARNING MESSAGES and FAILURE  MESSAGES     PROGRAM INTRODUCTION    9 22    R ESPINAR S L  AEi 01    To edit data of a program is necessary to start from an initial situation of controller shown    and follow the next steps           Connect the autoclave to the mains  and press the main switch  3  position   and at the  same time will light    The micropro
2.      FILLING OF THE CONDENSATION TANK    Unscrew the cap of the water inlet  7     Fill in the condensation tank with distilled water to the maximum level  until the message of  the screen disappears and you hear the acoustic signal  Put the cap again without tighten  it    Open the lid    Introduce the material   See LOADING THE AUTOCLAVE     The water to be used must have a minimum conductivity of 3 3 uS cm  maximum  resistivity 0 3 MO cm   If the value is lower  the level sensors may not work due to the low  conductivity of the water  In this case  and if there are not other means  mix a glass of tab  water with the distilled water and it will give conductivity to the water     Close the lid of the autoclave by means of tighten the closure   12 22    R ESPINAR S L  AEi 01    Select the appropriate program  press  START STOP  switch  initializing the process of  sterilization     The microprocessor screen will keep you informed of every step of the process     The sterilization cycle of a solid without dry is composed by the following phases                    The sterilization cycle of a solid with dry is composed by the following phases     13 22    R ESPINAR S L  AEi 01                      MPLETED    The sterilization cycle of a liquid is composed by the following phases                       VACUUM  Initial vacuum  air expulsion of the sterilization chamber   FILLING  Automatic filled of distilled water in the sterilization chamber   HEATING  Increase of pressure  heat
3.    R ESPINAR 8 1     R ESPINAR  S L     AEi 01    REGISTERED FIRM  ISO 9001             INSTRUCTION MANUAL  FOR USE AND MAINTENANCE    AUTOCLAVES FOR STERILIZATION     MICROPROCESSOR CONTROLLED  WITH DRY AND PREVACUUM     Models     AE 28 DRY  AE 75 DRY  AE 110 DRY  AE 150 DRY       1 22    R ESPINAR S L  AEi 01    This manual has been written for safety reasons  read the instructions    carefully before installing and  or using this apparatus     If this apparatus should be sold or transferred  ensure that this manual is    given to the new owner  for the correct use and installation     This manual should be kept by the apparatus for in case of doubt in its use    and for maintenance reasons     INDEX   Descriptio       I           Rearview                          idl elite    eae page  Characteristics and microprocessor functions                  page  Technical specifications                          ere etd ce        eaten idea eee        page  Characteristics         e p n a              page  ACCESSONICS      a iid ain va oe a an ee a ae page  Installation                      dade idee Aa ee      ee AT ee ea gee page  Connection to the mains                        i ede eee ied page  Changing voltage         e de page  Programming Mode                        Eu bee eee elena page  Alphanumeric display functions                                                   Program introduction           an ai ae ea page  Time and date adjust     bee e etae eel ee ee page  Program
4.  1 to sterilize solids glass   plastic  etc  and it will be set  LIQ 1 to sterilize a liquid  culture media     If the autoclave has not the accessory  Heart probe  and it is set by mistake SOL 2 or LIQ   2  when the sterilization cycle initialise  the program will be aborted and it will appear the  message     PROBE 2 FAILURE     On the other case  if the autoclave has this accessory  Heart probe   it can be editable  SOL 1 SOL 2 and LIQ 1 LIQ 2  depending on the usage of the device    With the heart probe installed in the autoclave  we can introduce it inside a liquid or in the  middle of a residues bag  according to that the 1 probe controls and maintains the    11 22    R ESPINAR S L  AEi 01    sterilisation temperature selected  the 2 probe   heart probe  controls the real temperature  of the liquid to sterilize  and until this liquid has not reached the selected temperature will  not count the time of sterilization  For this reason it can be possible to sterilize a product in    time and real temperature without mass or volume dependence     START UP INSTRUCTIONS    Before starting up the autoclave  check inside if there are residues left  plastic  paper  etc    Connect the autoclave to the mains and switch the main button  3  to the position I    At the microprocessor screen will appear the message   RAYPA STEAM STERILIZER   and a few seconds later will appear a number of a program  it will always be the last  program executed   and the message   LACK OF WATER     ET  
5.  93 731 37 17    Web site  http   www cecot es raypa E mail  raypa cecot es       22 22       
6.  disconnect the apparatus from the mains    Please get in touch with your distributor if in doubt    For safety reasons and a good function of the apparatus use original spare  parts only    If spare parts are needed state the serial number and the model of the    apparatus  indicated on the characteristic plate at the rear of the apparatus      N GUARANTEE  This apparatus is guaranteed for a one year period  against any fabrication failure or  defective part  The guarantee does not cover any casualty produced by improper  use of the apparatus or alien casualties to R ESPINAR  S L   If the apparatus is manipulated by non authorized personnel by R ESPINAR  S L      this guarantee is automatically expired        R Espinar S L  reserves all right to change technical specifications  without    previous notice        21 22       R ESPINAR S L  AEi 01    DECLARATION OF CONFORMITY CE     The manufacturer  R  Espinar  S L    declare under its own responsibility that the apparatus  Autoclaves for sterilization   models  AE 28 DRY  AE 75 DRY  AE 110 DRY  AND AE 150 DRY  Cod   13360028  13380075  13400011  and 13420015    fulfil with the directives of security  73 23 CEE  Low Tension  and 89 336 CEE   Electromagnetic compatibility    According to International Regulations EN 61010 1  EN 61010 2 041  EN 50081 2  and EN 50082 1    R  Espinar  Manager Director          R  Espinar S L     Av  del Valles 322 P I  Els Bellots   08227 TERRASSA  Barcelona   Tel  93 783 03 44 93 783 07 20   FAX 
7. ESCRIPTION       1   Operation mode  SOLIDS LIQUIDS    2   Number of the program    3   Sterilization temperature    4   Final drying    5   Sterilization time    6   Dry time    7   Switch for sterilization temperature selection   TEMPERATURE    This switch is used during a sterilization program edition to select and edit the figures   8   Switch for sterilization time selection   CLOCK    This switch is used during the edition of a sterilization program to select and edit different  figures    a Time of sterilization    a Type of sterilization    a Dry time        a Date Hour    9   Increase switch    In program mode it is used to increase the value of the parameter in edition   Out of program it is also used to select a program    10   Decrease switch     4 22    R ESPINAR S L  AEi 01    In program mode it is used to decrease the value of the parameter in edition    Out of program it is also used to select a program    11   Programming switch   PRO    While pressing this switch during 2 seconds you can access to the edition of the program   During the process of sterilization will shoe the program while pressing    12   Start Stop switch    This switch is used to run or to stop the sterilization program selected    To stop the sterilization cycle it must be pressed the START STOP switch during 2  seconds  If this operation is made  in the final onform will appear the message  STOP   instead of the message  STERILIZATION OK      TECHNICAL SPECIFICATION    Apparatus  Steam s
8. N THE OF AUTOCLAVE     To switch off the autoclave press the general switch  3  position 0  When you start up the    autoclave again this will always show the last program selected     LOADING THE AUTOCLAVE    Place the material to be sterilized directly or in baskets  in a way that you leave space the  steam to circulate freely    Take care not to cover the opening at the top of the autoclave    If special bags are used  make sure that they don   t touch the walls of the autoclave  because when using a drying cycle they might burn    We also recommend that the bags should be placed side plastic face down in baskets  because if placed paper side down they will absorb the condensation from the baskets     15 22          R ESPINAR S L  AEi 01    PRINTER MODE    The microprocessor comes out from the factory with the configuration of manual printer   To make possible printing a sterilization inform  if the printer has a switch called  PRINT    will only have to press it    To print an inform manually from the microprocessor    1   Keep pressed switch    TEMPERATURE      7   during some seconds  in the screen will  appear the message    MANUAL PRINTING     2   Press the switch  11     PRO     in the screen will appear   PRINT NOW             3   Press the switch  9   INCREASE   in the screen will appear   PRINT NOW  YES    4   Press the switch  11     PRO    in the screen will appear     PRINTING     and it will give us  an inform of the last sterilization without data  as the fol
9. at shows the possibility to re   start the cycle again  This situation will take place after the execution of a program of  liquids or when the cycle has been aborted in a step where the pressure was not enough  to take out the water in a solid program    It can also be a failure   See FAILURE MESSAGES     FAILURE MESSAGES    The failures detected during the sterilization process  a part from giving you the printing  warning  are accompanied with the sound of an acoustic signal and the light of LCD   Pressing the switch    START STOP     the sound stops  When pressing the switch will  disappear the failure warning message also  and the lights of the LCD   in case you don t  have to do a discharge phase  program LIQUIDS   if the cycle was in a determined phase   the control still consider to be pressure inside the autoclave  or the temperature is still  high  in the case of liquid programs when the cooling process is finished or the discharge     the acoustic signal will sound     FAULT PROBE 1  The control detects that the temperature probe 1  main  is broken  It is impossible to  proceed with the sterilization  If during the sterilization the probe is broken the process    stops     FAULT PROBE 2   The control detects that the probe 2  heart probe  is broken during a sterilization process  with two probes  If this happens during a cycle  the sterilization is aborted    In this case it can be continued the sterilization while the probe 2 is changed  by editing  the programs a
10. cessor screen will show the message     RAYPA STEAM STERILIZER     during 2 seconds     When the autoclave is running for the first time  the screen will show in the first line the n      of the program  the temperature of sterilization and the sterilization time  in the second line  of the message     LACK OF WATER     because it is not filled yet the distilled water  condensed tank  See START UP    When is going to be edited a program  the message    LACK OF WATER  and will appear    the parameters left to complete the rest of the program                 Select with the buttons  9     INCREASE            10     DECREASE     the number of the  desired program    Press the switch n    11     PRO    during 2 seconds to get into the program editor desired   and it will be shown the letter    P    to indicate the edition situation    Press the switch n   7     THERMOMETER  to edit the sterilization data    Pressing the switch n   8   CLOCK  will be selected the time of sterilization or the field  SOL LIQ  corresponding at all kinds of sterilization to realize  or also the field SEC   which corresponds to the dry time    Once the field to edit is set  use  Increase  and  Decrease  switches to modify the  values of edition    To validate the modified data  press    PRO    switch  finishing the program edition    If it is needed to go out from the program without validating the modifications done   press the    START    switch that is used as an exit switch of the program  the cont
11. eal time and  temperature  not depending on the volume or capacity of the  material to sterilize   Thermal printer of continuous sheet  that prints the number of  the program  number of cycle  temperature  time and date of    every sterilization     Description   Perforated stainless steel basket AISI 316 of  280x200mm for  AES 28   2 per autoclave    Perforated stainless steel basket AISI 316 of  380x260mm for  AES 75 and for AES 110   2 per autoclave AES 75 and 3 per  AES 110    Perforated stainless steel basket AISI 316 of   475x360mm for  AES 150   2 per autoclave    Non perforated stainless steel basket AISI 316 of   280x200mm  for AES 28   2 per autoclave     6 22    R ESPINAR S L  AEi 01    Code Ref  Description   90130775 CCI 75  Non perforated stainless steel basket AISI 316 of   380x260mm  for AES 75 and AES 110   2 per autoclave AES 75 and 3 per  AES 110    90130850 CCI 150 Non perforated stainless steel basket AISI 316 of   475x360mm  for AES 150   2 per autoclave    90130740 CEP 7040 Pippete sterilize cylinder of   70x400mm   90131340 CEP 1340 Pippete sterilize cylinder of   130x400mm   90131326 CEP 1326 Petri capsules sterilize cylinder of   130x260mm   90131347 CEP 1347 Petri capsules sterilize cylinder of   130x400mm   90131428 SRA 1 Height adjustable support with shelves for AE AES 28   90131475 SRA 2 Height adjustable support with shelves for AE AES 75   90131510 SRA 3 Height adjustable support with shelves for AE AES 110   90131550 SRA 4 Height adjustable s
12. ing   UNLOADING  Automatic unload of the water and steam of the chamber to the  condensed water tank   only in solid program    DRYING   Baric balance and drying vacuum   only in solid program    COOLING  Natural cooling process  after the sterilization period and up to  90  C   only liquid program    PROCESS COMPLETED  End of cycle signal     14 22    R ESPINAR S L  AEi 01    At the end of the sterilization cycle  END OF PROCESS  sounds an acoustic signal  To  come back to the initial screen just press switch    START STOP       After the acoustic signal of  END OF PROCESS     and if the cycle done is with dry  the  sterilization chamber has vacuum yet that is shown on the manovacuometre  4    Depending on the autoclave s model  it still needs some time to re establish the  atmospheric temperature  it could be 10 minutes in models of 1501    Do not try to open the lid while the manovacuometre  4  is not showing  0    Once the manovacuometre shows  0  open the lid and keep it opened for a couple of    minutes before taking out the material           DO NOT INITIALIZE ANOTHER CYCLE OF STERILIZATION  BEFORE HAS PASSED 15 MINUTES  FROM THE LAST CYCLE   LET THE LID OPEN DURING THIS TIME        If the cycle done before was of liquids  in the screen will be possible to read    WATER IN CHAMBER    alternatively with  START TO CONTINUE   See  ADVISE  MESSAGES              DO NOT OPEN THE AUTOCLAVE UNTIL THE CYCLE  OF STERILIZATION IS FINISHED AND THE MANOMETRE SHOWS 0        SHUT DOW
13. lowing example    DATA PRINTING RESULTS          In automatic printing mode  data concerning to temperature is suitable  step of the cycle   and also the time of every step lasted and a final inform    To set the automatic printing    1   Keep pressed the switch  7   TEMPERATURE  during some seconds  in the screen will  appear the message   MANUAL PRINTING     2   Press switch n   9   INCREASE   and in the screen will appear   AUTOMATIC  PRINTING     3   Press switch n   11     PRO     and in the screen will appear  DATA CADENCE     this  means that this will print data every 120 seconds  To modify the printing times press  switches  INCREASE DECREASE       16 22    R ESPINAR 8 1  AEi 01    4   Press switch n   11     PRO     in the screen will appear   PRINT NOW      Press again  switch n   11   PRO  and it will appear in initial screen    With this mode of printer  every time the sterilization cycle is initialized  the printer   automatically will print data with a cadence of printing previously set by doing the  following    DATA PRINTING RESULTS                17 22    R ESPINAR S L  AEi 01    PROTECTED PROGRAMS  Info      The autoclave comes from the factory with 4 standard programs of sterilization   memorized in programs PO to P3  and they are protected  that means impossibility to  change them   Otherwise  to have access an block and unblock the N  of programs of our choice  we  have to follow the next steps   1   Keep pressed the switch  7     TEMPERATURE    during s
14. ming of printer connection 2    page  SOLID LIQUID Modes         ii page  Statup A c  page  Filling of the condensation tank   1                     page  Shut down of the autoclave               ssssssssseeeeeneneeeenen menn nennen nennen enne enne page  Loading the autoclave    ssepe cete ee        i ere nae page  Printerdode         use Re pic ep ep vc et edet He ee ee Ld          page  Protected programs  Info         eee Pise bee iin pite bike page  Warning ImessageS uuo ode ee eee eec duet ecce en deba eer pi e gb de e edd page  Failure massages                                    ave indeed eed page  Repairs and spare parts         iius        ede ede dor ie iene Ra ka eade ce eiae de a qr anne page  Guarantee    idee denied ade lads nee eee eee ie page  Conformity declaration                 nennen nemen nennen neret nnns page                          Oo Oo TO KR WO       11  11  11    12  15  15  16  18  18  19  21  21  22    2 22    R ESPINAR 8 1  AEi 01  DESCRIPTION    1   Interface outlet RS 232   2   Microprocessor     3   Mains switch        4   Mano vacuometre    5   Thermal printer of continuous sheet   6   Thermal protector of the cover    7   Filling of the condensed water tank     REAR VIEW    8   Ventilation bacteriological filter  10  9   Safety valve     11  10   Condensed water tank empty valve  12       11   Condensed tank outlet   12   Valve for autoclave chamber empty     3 22    R ESPINAR S L  AEi 01    MICROPROCESSOR FUNCTIONS AND CHARACTERISTICS  D
15. ome seconds  in the screen will  appear the message   MANUAL PRINTING  and then the next 4 seconds will appear in  the screen the message   N PROG PROTEC  4  that shows us that the 4 programs are  protected   2   Pressing switches  INCREASE DECREASE  to modify the n  of program that is  suitable do be block or unblock   0  1 To 10   Program to protect from PO to P9     No program protected     3   To exit the screen and validate the n  of programs protected press switch  PRO      ANG THIS INFORMATION IS CONVINIENT TO BE KNOWN BY AUTORIZED  PERSONNEL ONLY     WARNING MESSAGES    These messages will appear in the second line of the microprocessor screen  They only    appear if there is a sterilization process going on     LACK OF WATER   It is detected that the water of the condensed tank has reached its minimum level    This message will not disappear until the maximum level would be replaced  Fill in with  water just up to the point the message disappears  This will coincide with an acoustic    signal     PRINTING    This message will remain until the printing process is finished     REPLAGE FILTER    18 22    R ESPINAR S L  AEi 01    Remind you to change the bacteriologic filter  An acoustic signal will sound intermittently   To delete this message it must be stopped and started with the main switch  3      WATER IN CHAMBER   The control detects the water inside the sterilization chamber  out of cycle  This message  will be alternative with another   START TO CONTINUE     th
16. roller  goes back to the normal situation without validating any change in edition    Switches  INCREASE  and    DECREASE     make no effect over the edition data  if the  selected program is protected    Have in mind that       If the program is for liquids  field SEC will not exist  and for this reason will be  modified         If SEC is equal to    0     out of the program edition this field will not appear     DATE AND HOUR ADJUST    10 22    R ESPINAR S L  AEi 01    Starting from the initial position of view  maintain pressed during 5 seconds the switch   CLOCK   and the year will be lighting   pressing some times the switch  PRO  we will  have access to the other parameters  month  day  hour and minute     To modify the parameter to edit  press switches  increase decrease     Go out from the  configuration by pressing switch  PRO     To visualize date and hour  just press the switch    PRO        PROGRAMMING OF PRINTER CONNECTION    The autoclave is provided with a lateral connector  1  of 9 pins   for printer connection   The printer which is connected  will be of 24 columns and must have a RS 232 interface at  9 600 bps  8 bits without parity and 1 bit of stop  It also must have a minimum buffer of 150    characters        9 PINS CONNECTOR    If a printer is connected  see paragraph  PRINTER MODE   SOLID LIQUID MODE    If the autoclave is not equipped with an accessory code number  90131400  reference  PT   2  Heart probe   when a program is edited it will be set  SOL
17. s SOL 1 and LIQ 1     19 22    R ESPINAR S L  AEi 01    FAULT FILLING   When the filling phase is initialized  the control makes the autoclave fill in with water up to  its level  by acting over the filling valve  The control takes the time to fill in the chamber    If the autoclave is not filled within a determined time  or if it is observed a failure during this    process  or also a failure at the level detector  the cycle is aborted     FAULT HEATING  When a cycle of sterilization starts  the evolution of the temperature increase is being  controlled up to the temperature selected  If the temperature does not evolute correctly     that means there must be a problem and the cycle is aborted     FAULT TEMPERATURE  During the sterilization process the temperature must be between a scale of value to  guarantee a correct sterilization   if this value is modified more than the setting one  the    sterilization is not correct and the process is aborted     ELECTRO CUTTING  By the way it is not a failure but an interruption of the sterilization cycle  because of an    electrical cut  This will mean an abortion of the cycle     WATER IN CHAMBER  If at the end of the sterilization process  the control detects water inside the autoclave     after the execution of a solids program   LACK OF WATER    This message will not disappear until the autoclave has been filled up to its maximum    level     20 22       R ESPINAR S L  AEi 01    REPARATION AND SPARE PARTS    Before any reparation
18. terilization autoclave  Sterilization temperature  From 105  C to 139  C   Max  pressure  2 5 bar  Voltage  220 V single phase  for AE 28 DRY and AE 75 DRY    220 V or 380 V triple phase   N  for AE 110 DRY and  AE 150 DRY    Frequency  50 60 Hz   Consume  2 000 W  3 000 W    Model AE 28 DRY   Model AE 75 DRY   4 500 W  Model AE 110 DRY   6 000 W  Model AE 150 DRY   Sterilization time  from 3 to 120 minutes   Final Drying  Selectable   DRY NOT DRY   Drying time  From 0 3 to 60 minutes     Data printing  Manual   Printing cadence  Every 120 seconds   Printer connection  Interface RS 232    5 22    R ESPINAR 8 1     CHARACTERISTICS          Seal of silicone     AEi 01    a Tank  rotary seal and lid made of stainless steel AISI 316      Exterior metallic and top surface made of AISI 304 stainless steel     a Automatic air purge evacuation by initial pre vacuum    a Distilled water tank   with maximum and minimum water levels   a Drying system with dry heat and vacuum pump    a Automatic water filled in the sterilization chamber     a Over temperature and over pressure safety devices  safety presostate and safety    ACCESSORIES THAT MUST BE INSTALLED AT THE FACTORY     valve   ACCESSORIES  Code Ref   90131400 PT 2  90131300 IT    Other accessories     Code  90130228    90130375    90130450    90130628    Ref   CI 28    CI 75    Cl 150    CCI 28    Description  Heart probe PT 100  hermetic with flexible wire of silicone to  introduce it into the liquids or bags to sterilize at a r
19. upport with shelves for AE AES 150    All accessories must be installed in factory    A INSTALLATION    Install the apparatus on a stable and solid surface   Don t install the autoclave in areas where there is danger of fire or explosion   Don   t block or cover the rear ventilation grid     Connect a flexible tube to the rear outlet  11  for drainage     CONNECTION TO THE MAINS    There is the characteristics plate situated at the rear  indicating the voltage and power   Check to make sure that your electrical installations have the same conditions    The plug of the oven is a SCHUKO with central and lateral connection  for security  reasons the oven must be installed to ground    The triphasic autoclaves  AE 110 DRY and AE 150 DRY  have a normalized plug of 16A   3 poles  neutral and ground    The autoclaves are equipped from the factory to be connected at 380V N and ground  but  they can be changed into triphasic 220V and ground following the instructions given in  CHANGE OF VOLTAGE    CHANGING VOLTAGE    7 22    R ESPINAR S L  AEi 01    To change voltage into 220V triple phase and ground follow the next steps   a Disconnect the autoclave from the mains       Take off the back cover of the autoclave       a Change the metallic bridges on the binding post from the star connection to a delta    connection  as indicates below       Take out the neutral  blue  from terminal space of main entrance  and connect to black    wire L1                                                 
    
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