Home

Instruction Manual of Rotors and Tubes

Contents

1. 15 2 7 Life The life of plastic tubes and bottles depend upon the sample speed of rotor temperature etc The standard life of plastic tubes and bottles is specified as follows PET tubes PA seal tubes re seal tubes and cone top tubes are used one time only Table 2 3 Standard life of plastic tubes kk Used at Used at over Used with wea alkali sample standard condition 70 000 rpm Autoclaving 5 times 1 times 5 times 5 times 5 times 5 times 5 times 5 times 5 times Thick walled 50 times 50 times 50 times tubes Bottles 20 times 5 times 10 times In case they are used repeatedly for 24 hours at the maximum speed of rotors below 70 000 rpm with the aqueous sample pH7topH9 Tubes capped with S series caps are disposable For caps space caps adapters and crowns check for every use and replace worn or deteriorated one N CAUTION Do not use tubes bottles that have exceeded their life expectancy Failure to do so could result in damage of tubes bottles and the rotor and the centrifuge The life expectancy of tubes bottles depends on factors such as the characteristics of samples speed of the rotor used and temperature Always check for deterioration and damage cracks deformation and so on on tubes bottles before using them Do not use the tubes bottles if you find such a problem 2 8 How to use tube and bottles e Tubes 5mL and over for angle rot
2. SRP50AT 6 5PA Seal tube 5407828 P100AT2 P50AT4 P100AT RP50ATA RP55 SRP50AT RP40 3 RP40 2 B5 Space cap red 12PA Seal tube 484285 P65A P65AT RP65T RP65 RP40 C Space cap white RPW65T RPW45 P55AT 486582 P90AT P80AT P70AT2 RP83T RP80T 3 C2 Space cap red SRP70AT 486583 C3 Space cap white RPV45T 5402310 P65VT3 P65NT RP55VF RP65VT3 C4 Space cap black RP6S5NT 40PA Seal tube 485649 P70AT P50AT2 P50A2 P30A2 RP70T RP50T 2 RP50T RP50 2 RP30 2 RPW50T RP60T E Space cap red 484252 E2 Space cap blue RPV50T RPV30 5402525 E3 Space cap white P50VT2 RP50VF 94PA Seal tube 5408969 F Ti Space cap red P45AT P42A RP45T RP42 RPW35 The tube sealer model STF 1 model STF2 or model STF3 and the tube rack are necessary to seal the tube Applicable seal tubes 1 5PA seal tube 2PA seal tube 3 5PA seal tube Tube rack B2 201778G 201778F 201778E 4PA seal tube Tube rack B3 201778H 5PA seal tube 6 5PA seal tube Tube rack B4 201778A For STF 1 STF2 STF3 201778J 8PA seal tube Tube rack C2 201778L 12PA seal tube 40PA seal tube Tube rack C Tube rack E 201778B 201778C 94PA seal tube Tube rack F 201778D For STF 1 Tube rack F2 201778M For STF2
3. below q ve beti N N Speed used rpm Nmax Maximum speed rpm Ky K factor at the speed used KK factor at the maximum speed 2 Tubes bottles and caps 2 1 The kind of tubes bottles and caps eTubes and bottles OTubes thin walled tubes For angle rotors or vertical rotors tubes must be filled up and used with caps For swing rotors tubes must filled to within 3mm from top of tubes and caps are not used SS tubes can be used with optional volume oThick walled tubes Thick walled tubes are used in angle rotors and swing rotors without caps For angle rotors these can be used with optional volume less than net volume For swing rotors fill tubes upto within 3mm from the top of tubes with a sample OSeal tubes Seal tubes are heat sealed and used with space caps in angle rotors neo angle rotors and vertical rotors The tube sealer model STF 1 model STF2 model STF3 and the tube rack are necessary to seal these tubes ORe seal tubes and Cone top tubes we discontinue to sell them Re seal tubes and cone top tubes are sealed by screwing a plug into the tube and used in angle rotors neo angle rotors and vertical rotors with a plug and a crown These tubes must be filled up the sample Specified tube vise and tightening tools are necessary to seal these tubes Registered trademark ot Seton Scientific Company U S A Space t Plug EY cap er Thick walled Seal tube Re seal tube Tube Cone top tube Fig
4. 2kgf cm E4 AL Cap can be used only for a 40PE tube 4 Loose the handle A of the tube vice and remove the tube with the tube setter Tube setter Use the screw side 19 20 4 Balancing 1 Fill up the sample with the injector 3 Tighten the setscrew securely with etc the tube setter Set screw Tube setter Use the hex wrench side 2 Keep the difference of any two tubes which are arranged symmetrically within 0 3g See 2 9 How to balance Tighten the setscrew to prevent the leakage of the sample When using CP NX CP WX CP MX and CP a B series centrifuges you can balance the tubes simply by leveling the sample e Tubes 4mL or less for angle rotors Preparation Ea lt Tube gt lt Cap gt lt Sample gt lt Tube vice gt lt Box wrench gt lt Injector gt 7 Preparing the cap 1 Confirm the composition of the cap 2 Fix the tube vice to the desk etc by turning the handle B Desk etc Handle B 2 Preparing the tube 3 Assembling the cap Match the marks of the stem and the crown and screw the nut by hand Fill the tube upto 5mm from the top of the tube with the sample Nut 21 22 5 Fitting the cap 4 Balancing Put the tube and the cap in the balance keep the difference of any two tubes which are arranged symmetrically with in 0 3g When using CP NX CP WX CP MX and CP a B series centrifuge
5. 3 and the rotor instruction manual 56 4 Is the cover or the cap of the rotor tight Is the cap of the tube tightened firmly When using seal tubes is the tube sealer adjusted properly heater height centering See the STF 1 instructions the STF2 instructions or the STF3 instructions Are burrs removed after welding See the STF 1 instructions the STF2 instructions or the STFS instructions Is the tightening torque of the caps of the neo angle rotors or the vertical rotors right See 3 6 Neo angle rotors and titanium vertical rotors 5 Aren t parts like the cap deteriorated or worn See 2 6 Inspection Deteriorated or worn parts must be replaced 6 Is the sample and detergent good See 2 2 Materials of Tubes Are sterilizing conditions right See 2 4 Sterilization e If the tube is squeezed fill the tube hole on the rotor with tepid water and after a few minutes pull out the tube by using tweezers or something like that e If there is only a trifle symptom of trouble locate the cause or call a Hitachi Koki authorized sales service representative If such a symptom is overlooked an accident might result e If the cap has fallen in the bottom of the tube hole and you cannot removed it call a Hitachi Koki authorized sales service representative When calling a Hitachi Koki authorized sales service representative keep the part of intere
6. 34 2 9 How to balance Balance any two tubes with balance which sensitivity is less than 200mg accessory of centrifuge When using three tubes at swing rotor Balance each of three tubes But using CP NX CP WX CP MX CP a B series centrifuge you can balance the tubes simply by leveling the sample N CAUTION Extremely different sample quantities must be avoided Failure to do so might damage mechanical components N CAUTION Do not load only one tube or load tubes asymmetrically Asymmetrically loading may cause imbalance operation and damage the centrifuge and rotor Balancing two tubes Balancing three tubes When using three tubes at swing rotor Fig 2 6 How to balance N CAUTION Be careful that imbalance operation may occur in the following cases Fill the same sample in the tubes bottles and load them in the rotor ouckets that are placed symmetrically with respect to the drive shaft in the rotor Oi samples that are equal in volume but different in composition are used the precipitation levels may be different by centrifugation and such operation may increase the level of imbalance Di samples that are equal in weight but different in volume density are used or if the tubes bottles are different in inside diameter or shape there may be variations in position of center of gravity and such operation may cause imbalance The tubes of the combination in Fig 2 7 shall not set symmetrically because occurri
7. 474040A 474041A S308431A P45AT P42A RP45T 70PC Bottle B PPO PPO NBR PC RP42 RPW35 308405A 6pcs set 486370 474040A 474041A S308431A PASAT 70PC Bottle C AL PPO NBR PC 310331A 6pcs set 486370 S413195A 474041A S310419A 70PA Bottle C AL PP NBR PA S413248B 2pcs set S310455 474555 474633 AL Cap 2 assy AL POM NBR S403617A 1pc set 8301384 S401808A S401809A AL Cap assy AL POM NBR Nominal capacity The caps for 230 PA bottles P70AT P50AT2 P45AT P21A2 P19A RP19 64 Appendix 6 The list of tube kits eOpen top Tube Kit CAP PACKING for CAPS TUBE VISE WRENCH TUBE SETTER Parts a 4361463 D pe Mer 469283 d Kits Part No Name PartNo Name PartNo Name PartNo Name PartNo Name PartNo Name E 5304985A 329445A 2 460696A 8 S406626A 2 348253A 1 4361453 1 435823A 1 6 5PA Tube Kit 6 5PATube 100 B TI Cap 20 Tube Vise B Box wrench Tube Setter 5304986A 329606A 2 463577A 12 401803A 2 333885A 1 469283 1 435823A 1 12PA Tube Kit A 12PATube 100 ZT Cap 20 Tube Vise C2 Torque wrench Tube Setter 5304986B 329606A 441399A S401796A 300714A 4361453 435823A 12PA Tube Kit B 12PA Tube C AL Cap Tube Vise C Box wrench Tube Setter 5304987A 329607A 339673A S401787A 343417A 4 435823A 40PA Tube Kit A 40
8. 5401796A CR 453412A AL 5401791A NY C4 AL Cap S407404A 5401829A SS 5401803A NBR 5407405A AL 5401791A NY C TI Cap 463577A 5401829A SS 5401803A NBR 463576A Tl 5401791A NY C2 TI Cap 481649A C SS Cap 413312A 5401829A SS 5401829A SS 5401803A NBR 481650A Tl 453413A SS 5401791A NY 5401791A NY E AL Cap 321517A 5401829A SS 5401813A POM 459814 AL 5401787A NBR 453416A AL 5401791A NY E2 AL Cap 331198A 5401829A SS 434022 AL 459814 AL 5401787A NBR 453416A AL 5401791A NY E3 AL Cap 305231A 5401829A SS S408401 AL 5401813A POM 5408400 AL 459814 AL 5401787A NBR 5408421A AL 5401791A NY E4 AL Cap S308090A 5401829A SS 5408401 AL 5401813A POM 5411239A AL 459814 AL 5401787A NBR 5408421A AL 5401791A NY E TI Cap 474272A 5401829A SS 474277 474276 474275 TI 5401787A NBR 474273A TI S401791A NY E TI2 Cap 339673A 5401829A SS S401811A POM 459814 5401787A NBR 478421A AL 5401791A NY E SS Cap 451480A E2 SS Cap 418763A F AL Cap 322690A 5401829A SS 5401829A SS 5401829A SS S401811A POM 5401792A NBR 453417A SS 453417A SS 453414A AL 5401791A NY 5401791A NY 5401791A NY F2 AL Cap 325968A 5401829A SS S401811A POM 5401792A NBR 453414A AL 5401791A NY F
9. O Replacement of consumable items Parts subject to a restricted warranty period that has expired eOther Matters Rotors that are delivered to Hitachi Koki for inspection or repair must be sterilized and cleaned beforehand Be aware that Hitachi Koki will sometimes decline to carry out inspection or repair if a rotor or other related equipment is or could be contaminated as a result of use without protection or under inadequate safety management in a radioisotope facility or a laboratory of the level P2 or higher 68 Decontamination WARNING If the centrifuge rotor or an accessory is contaminated by samples that are toxic or radioactive or blood samples that are pathogenic or infectious be sure to decontaminate the item according to good laboratory procedures and methods GE there is a possibility that the rotor or an accessory is contaminated by samples that might impair human health for example samples that are toxic or radioactive or blood samples that are pathogenic or infectious it is your responsibility to sterilize or decontaminate the rotor or the accessory properly before requesting repairs from a Hitachi Koki authorized sales service representative Note that Hitachi Koki cannot repair the centrifuge rotor or the accessory unless sterilization or decontamination is completed GR is your responsibility to sterilize and or decontaminate the rotor or parts properly before returning them to a Hitachi Koki authorized sal
10. SS Cap 418759A NOTE 1 the upper part No 5401829A SS the lower materials 5401797A CR NOTE2 Set screw washers O rings gaskets and inserts are available in sets of 10 453415A SS NOTES Individual nuts crowns weights and stems are available The stem is provided with an insert 5401791A NY 59 Appendix 2 The list of S Series Cap and tools S Series Cap For 12PA 12PE 40PA 40PE tubes e Component eMaterials AL Aluminum alloy TI Titanium alloy eNames of parts NY Nylon gt _ Ke Setscrew Groove ALStem Ti Ring This is a mark for up and down direction AL Stem is provided with an insert Parts Set screw AL Stem Insert Ti Ring Caps S 12AL Cap 5410543A 8401791A S410545 S410542A 94018234 AL NY TI S 40ALCap 410533A 8401791A 8411473 S410532A 54018294 AL NY TI NOTE1 the upper part No the lower materials NOTE2 Setscrew and inserts are available in sets of 10 NOTES Individual AL Stems and Ti Ring are available S Cap Tool Set The caps are not included in S Cap Tool Set Please purchase applicable ones Tube Holder E Tube Holder C Tube Setter C Hand Press c Parts of the tool set prie ap plied rotors Toolset Tube Holder Tube Setter Hand Press S 12ALCap eder derC P90AT P80AT S410542A 5308625A Eee 481056A S204591A P70AT2 S Cap Tool Set 85411486 Tube Setter C
11. STF3 2PA 4PA 5PA 6 5PA 12PA and 40PA seal tubes Type of tube rack is stamped on top of the tube rack e g In case of Tube rack G G is stamped on top of the tube rack 62 Multi rack For STF 1 STF2 STF3 The 1 5PA 3 5PA 8PA and 94PA seal tubes cannot be used in the multi rack 201778K Tube rack Appendix 4 lt Tube vise gt Caps tightening torque kg cm A2 AL Cap 336710A lt Box wrench gt Tool sets lt Torque wrench gt 469283 474871 Parts of the tool set Tube vises S303696A Tube vise A2 Box wrenches Toraue wrenches S406641 Box wrench Tightening tools of the cap f Ah lt Torque wrench gt 477456A Applied rotors Old models A3 AL Cap 336711A S303696A Tube vise A2 S406641 Box wrench S300715A 348253A 4361453 P50AT4 RP50AT4 Tool set B Tube vise B Box wrench SRP50AT B AL Cap 336662C 4361453 414429A Tube vise B Box wrench For 5PET tube S300716A S300717A 4361453 RP30 3 Tool set B2 Tube vise B2 Box wrench B2 AL Cap E 336662C 4361453 P50AT4 RP50AT4 474507A Tube vise B Box wrench For 6 5PET tube B TI Cap 300715A 348253A 4361453 460696A Tool set B Tube vise B Box wrench P100AT2 P100AT RP80T B2 TI Cap ES 336662A 469283 474070A 100 Tube vise B Torque wrench RPV65T C AL Cap 300713A 300714A 4361453 P65A P65AT P55
12. Tighten the cap until the bottom of the cap bumps to the bucket_and the cap must be adjusted to the match mark of the bucket Bucket stand 2 Set the tube to the bucket Be sure to set balanced tubes symmetrically ZA CAUTION Imperfect tightening of the cap may cause imbalance operation and it may cause the serious accident Bucket 43 3 Setting the bucket A WARNING When using swing rotor be sure to set all buckets whether or not samples are Put in buckets Failure to do so could not only cause the rotor to vibrate but could result in the rotor being deformed and the buckets becoming detached which is very dangerous Never use buckets made by other companies or any other type of bucket that is not exclusively made for the rotor even if it is made by Hitachi Koki 4 Operation N WARNING Before setting a rotor in ultracentrifuge make sure that buckets have been securely installed in the rotor Incorrect installation of buckets in rotor could damage the rotor or detach the buckets which is very dangerous 1 Gently and securely set the rotor onto the drive shaft 2 After mounting the rotor on the drive shaft check that the buckets swing smoothly by touching buckets gently 3 For operation refer to the instruction manual of the preparative ultracentrifuge 5 Removal of sample 1 Carefully remove the rotor from the drive shaft 2 Remove the buckets from the rotor 3 Remove the cap fro
13. and cap tight securely packing breaks or comes Isn t packing elongated out of position ls specified packing used The handle or the cap Is lubricant applied to threads cannot be tightened lf threads are defective call for repair The rotor pin is bent Call for repair The swing rotor s bucket hook is deformed Is the hook tightened at the wrong position set by the tightening tool lt should be repaired The swing rotor s bucket does not swing normally Is the bucket installed in reverse See 3 5 Swing rotors Is the bucket cap tightened securely Do the marks match Is the bucket type correct lf there is even a slight abnormality inspect the rotor again The rotor is stuck on the shaft Dust or something like that is sticking to the shaft or drive hole Call a Hitachi Koki authorized sales service representative The aluminum rotor s tube cavity or bucket have changed color Rinse sufficiently If white dots are found after rinsing inspection is necessary Consult a sales agent e f there is too much corrosion it may not be possible to repair it f an aluminum bucket old product is used with a swing rotor it could be corroded or broken unless it is maintained carefully lt is recommended to replace it with a titanium bucket 55 Symptom Possible causes and actions The reflector striped part of the op
14. either aluminum alloy or titanium alloy Table 1 1 shows the specifications of angle rotors and Fig 1 1 shows the name of parts Handle ie Handle Bar EES Handle Handle packing Ce gt Cover DSD Rotor packing Rotor body Cover Rotor body Optical adapter disk Drive hole or RLM adapter Fig 1 1 Names of parts of angle rotor Table 1 1 Specification of angle rotor Rotor Maximum Speed rpm Maximum R C F x 9 K factor Tube mLx Number Rotor body material Cover material P100AT2 100 000 803 000 18 65x 8 Titanium alloy Titanium alloy P90AT 90 000 700 000 25 12x 8 Titanium alloy Titanium alloy P80AT 80 000 615 000 27 12x 8 Titanium alloy Titanium alloy P70AT2 70 000 452 000 12x12 Titanium alloy Titanium alloy P70AT 70 000 505 000 40x 8 Titanium alloy Titanium alloy P65A 65 000 370 000 12x10 Aluminum alloy Aluminum alloy P50AT4 50 000 316 000 36 44 48 32 6 5 x 44 Titanium alloy Aluminum alloy P50AT2 50 000 303 000 70 40 x 12 Titanium alloy Aluminum alloy P50A3 50 000 252 000 33 1 5 x 24 Aluminum alloy Aluminum alloy P45AT 50 000 235 000 130 94x 6 Titanium alloy Aluminum alloy P42AT 42 000 223 000 12 0 23 x 72 Titanium alloy Aluminum alloy P32AT 32 0
15. of salt such as cesium chloride or the combination of tubes and bottles or tube adapters and caps Refer to this instruction manual d Always balance the tubes bottles and samples within the allowable imbalance of rotor including the tube adapters and caps Operation with imbalance exceeding the allowable range could damage the ultracentrifuge and rotor d Do not load only one tube or load tubes asymmetrically Asymmetrical loading may cause imbalance operation and damage the centrifuge and rotor d Clean the inside of drive hole crown hole in the rotor and the surface of drive shaft crown of centrifuge once a month If the drive hole or drive shaft is stained or if any foreign matter adheres the rotor may have been improperly installed and could come off during operation d Do not use tubes bottles that have exceeded their life expectancy Failure to do so could result in damage of tubes bottles and the rotor and the centrifuge The life expectancy of tubes bottles depends on factors such as the characteristics of samples speed of the rotor used and temperature Always check for deterioration and damage cracks deformation and so on on tubes bottles before using them Do not use the tubes bottles if you find such a problem d Maintain and inspect the rotor each time after using it If you fine any abnormality in the rotor stop using it and contact a Hitachi Koki sales service representative CONTENTS 1 Specification Of rotors err
16. other than the P32ST rotor and aluminum angle rotors are assumed to be in the first lifetime until they reach 1 000 operation times or 2 500 accumulated hours It is necessary to inspect rotors that have exceeded their first lifetimes at the works this is charged Then the maximum permissible speed should be decreased by 10 and the rotor is assumed to be in its second lifetime until it reaches 1 000 operation times or 2 500 accumulated hours P32ST rotor The P32ST rotor is assumed to be in the first lifetime until it reaches 500 operation times or 2 500 accumulated hours It is necessary to inspect the rotor that has exceeded its first lifetime at the works this is charged Then the maximum permissible speed should be decreased by 10 and the rotor is assumed to be in its second lifetime until it reaches 500 operation times or 2 500 accumulated hours The service life of RLM Rotor will be automatically calculated from the rotor speed and operating hours Namely if you use the rotor at a speed lower than its maximum permissible speed the service life in operating times and hours will be prolonged automatically by calculating from the strength of rotor material Titanium angle rotors Neo angle rotors and Vertical rotors The titanium angle rotors neo angle rotors and vertical rotors must not be used when they reach 5 000 operation times or 10 000 accumulated hours These rotors do not have a second lifetime For warranty see the end
17. rr rro 1 1 1 Preface eet ert etre tre rrr rer rer rer rerrerrerrerrerrerrerrrrerrerrerrerrerrerrerrerrerrerrerrerreny 1 1 2 Angle Dela 2 1 3 Neo angle elai 3 1 4 Swing FOTOS AAA AAA 4 1 5 Vertical 10 0 fs ttrt ttrtrrsrrnrnnnnnnnnnnnnnnnnnnnnnnnnnnunnnnnnnnunnnnunnnnnunnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnn 5 1 6 Prevention of over speed sunnnununnnnnnunvnvnvnvnnnnunvnvnnnvnnnnvnvnvnvnnnnvnnnvnvnnnnnnnvnnnnnnvnvnnnnn 6 1 7 Relation between rotors and ultracentrifuges eee eee eee eee eee ere ree ee ee ee rere rer etre 7 1 8 Calculation of separation CharacteriStics ttttttrrtss sr rn rss e eens 9 2 Tubes bottles and zlea 11 2 1 The kind of tubes bottles and 620 ZAUAN AAA 11 2 2 Materials of ee 13 23 Washing GANAT 1 3 2 4 Sterilization HARA 14 2 5 Storage etree terre rier terre rrerrerrerrerrerrerrrrerrerrerrerrerrerrerrerrerrerrerrerrerrerreny 15 26 Inspection HANNA AAO 15 27 Life GANA 16 2 8 How to use tubes and Mele 17 e Tubes 5mL and over for angle rotors and vertical OO 17 e Tubes 4mL or less for angle rotors urr 21 e Tubes 12PA 12PE 40PA 40PE tubes that are applicable GOO 23 o Gau 27 Seal OE 28 e Thick walled tubes for angle 0 0 s ode 30 e Tubes for SWiNg OO rr rr rr rr rr 31 e Re seal tubes and cone top tubes tit ttttrt ttt tr retire 32 29 How to balance GATAZ 35 3 HOW to use del dal dA 36 3 1 Allowable rotor speeds ee eee eee eee eee eee rere eee etre rere teeter ere ee rere rere eee etre rere 36
18. 00 111 000 12 x 32 Titanium alloy Aluminum alloy P27A 27 000 106 000 160x 6 Aluminum alloy Aluminum alloy P21A2 21 000 71 000 230x 6 Aluminum alloy Aluminum alloy P19A 30 000 55 100 230x 6 Aluminum alloy Aluminum alloy Nominal capacity 1 3 Neo angle rotors Neo angle rotors are made from titanium alloy Table 1 2 shows the specifications of neo angle rotors and Fig 1 2 shows the name of parts Cap gt Cap packing Tube cavity Rotor body Optical adapter disk Drive hole or RLM adapter Fig 1 2 Names of parts of Neo angle Rotor Table 1 2 Specification of neo angle rotor Maximum Maximum M Rotor i Rotor ZE ZE o ka mL Herr EEEa b bn bed P9ONT 90 000 646 000 10 5x 8 40 49 Gk Sei P65NT 65 000 402 000 23 12x10 120 96 eg gl PE5NT2 65 000 431 000 15 5x18 90 87 Gat ed Nominal capacity 1 4 Swing rotors There are three types of swing rotors depending on buckets types hook type buckets pin type buckets only P65ST and top loading type buckets only P32ST Table 1 3 shows the specifications of swing rotors and Fig 1 3 shows the name of parts Swing rotors hook type buckets and pin type buckets other than the P32ST rotor Rotor body d ap Ca Cap O ring or lt Casket Sa dde Gasket GRINN pa 4 E
19. 12 S407157 1 40PA Seal Tube Kit A 40PA Seal Tube 100 E Spase Cap Tube Setter 5304992B 345321A 2 58402525 a S407157 1 40PA Seal Tube Kit B 40PA Seal Tube 100 E3 Spase Cap Tube Setter 304993A 304310A 4 485650 6 58407157 1 94PA Seal Tube Kit 94PA Seal Tube 100 F2 Spase Cap Tube Setter Q ty pcs box 65 Applicable rotors Applicable rotors of every tube kit are shown below Applicable Rotors Kits P100AT2 P90AT P80AT P70AT2 P70AT P65A P50AT2 P45AT P32AT PSONT P65NT P65NT2 P100VT P65VT3 P65VT2 P50VT2 304985A 6 5 PA Tube Kit S304986A 12 PA Tube Kit A 304986B 12 PA Tube Kit B D D 304987A 40 PA Tube Kit A D 304987B 40 PA Tube Kit B D 304988A 94 PA Tube Kit D S304989A 5 PA Seal Tube Kit A D D 304989B 5 PA Seal Tube Kit B D D S304990A 6 5 PA Seal Tube Kit D 304991A 12 PA Seal Tube Kit A 304991B 12 PA Seal Tube Kit B 304991C 12 PA Seal Tube Kit C S304992A 40 PA Seal Tube Kit A 304992B 40 PA Seal Tube Kit B 304993A 94 PA Seal Tube Kit 66 Warranty eWarranty for Ultracentrifuge Rotors Hitachi Koki warrants its ultracentrifuge rotors under the terms and conditions set forth below 1 Scope of Application This warranty applies to failure or damage to the rotor due to rotor defects in materials or workmanship that are attributable to Hitach
20. 2 1 OBottles A container of the screw cap type is called lt Bottle gt a bottle Bottles with inner caps are for Bottle B Bottle C Bottle C2 high speed rotation Bottles with Plastic lt gt lt gt tip polyacetal screw caps are classified as tm Screw cap cap bottles B and those with metal aluminum alloy caps are classified as bottles C See Fig 2 2 and appendix 5 When a 30PC bottle C3 30PC bottle C 100 000 x g max G Force the liquid SR me cap a O ring 30PA bottle C 70PC bottle B 70PC Bottle bottle C ZOPA bottle C 80PC bottle C2 and 80PC bottle C are used at more than Bottle should be over the shoulder of the bottle Any volume which does not exceed the net volume is applicable at less than 100 000xg eCaps and adapters OCaps A Cap is used when a thin walled tube is used in an angle rotor or vertical rotor Be careful that different caps have different assemblies parts and materials see appendix 1 Tightening tools for caps are required see appendix 4 OSpace caps for seal tubes Space caps prevent deformation of seal tubes during operation and are made from aluminum alloy Be careful that different rotors use different space caps see appendix 3 OCrowns and Plugs for Re seal tubes or Cone top tubes Crowns and plugs prevent deformation of re seal tubes or cone top tubes and leakage from tubes during operation Crowns are made from aluminum al
21. 3 2 Sample limitation AAA 37 3 3 Adhesion of sample bea 37 3 4 Angle alie e AAA AAA AAA A 38 3 5 Swing jela aA AAA 40 3 6 Neo angle rotors and titanium Vertical rotors big etn Wine det giar eie iee eae ide gae ae die Gea ia 45 4 Maintenance dakaz badagi Gera uate cater dag deez dida kada EE EAEE 48 ER BAREA 48 4 2 Maintenance Of rotors ANO 48 4 3 Sterilizing rotors titer teeter terete etree rr rr rr rrrr 50 4 4 Inspection Of rotors gt gt gt gt srrrrtrrrrrenr eneren nere trennte rnern nenn non nnnnnn nn nnn non nnn nonner 50 4 5 Replacing optical adapter disk urr rr 51 4 6 Life e KAN EA 5 Troubleshooting ede a E E E Oa ae EE cued bak ade EE Ge BSR GEEAE EE FOT 56 Appendix 1 The list of caps AEA 58 Appendix 2 The list of S Series Cap and tools urruz rr rr rr rrrrr 60 Appendix 3 The list of space GOES rn rn rn rr rrun 62 Appendix 4 Tightening tools of the Gu rrrrrrrrrrrnrrrrrrrrrrrrrrrrrrrrrr 63 Appendix 5 The list of boilee rrrr rr rn rn rrr rr rrrrn 64 Appendix 6 The list of tube bibra rr rr rrun 65 AES 67 Decontaminatjon AE 69 Rotor retirement GANAT 69 1 Specification of rotors 1 1 Preface The rotors can be classified into following matters 1 Classified into shapes Angle rotors This rotor holds the tubes and bottles at an angle to the axis of rotation It is mainly used to separate components in a cell using fraction centrifugation Pelleting and separate nucleic acid using density gradient sedimentat
22. AT RP65T 441399A Tool set C Tube vise C Box wrench RP65 C4 AL Cap GO 333885B 4361453 P65A P65AT P55AT S407404A Tube vise C2 Box wrench For 12PET tube C TI Cap 341109A 333885A 469283 P90AT P80AT P70AT2 463577A 100 Tool set C2 Tube vise C2 Torque wrench SRP70AT RP83T RP80T 3 C2 Tl Cap 333885B 469283 481649A 100 SO Tube vise C2 Torque wrench RPV4ST C SS Cap 300713A 300714A 4361453 P65A P65AT P55AT RP65T 413312A Tool set C Tube vise C Box wrench RP65 E4 AL Cap 339198A 343417A 477456A P70AT P50AT2 S308090A 60 Tool set E Tube vise E Torque wrench For 40PE tube E3 AL Cap 339198A 343417A 477456A eee 5305231A 60 Tool set E Tube vise E Torque wrench RP50 2 P50A2 P30A2 RP30 2 E2 AL Cap 339198A 343417A 477456A RP30 331198A Tool set E Tube vise E Torque wrench E TI Cap 338956A 474871 474272A 120 E Tube vise Torque wrench ERRE E Tl2 Cap 339198A 343417A 477456A 339673A 60 Tool set E Tube vise E Torque wrench ZEREN E SS Cap 343417A 4361453 451480A GO Tube vise E Box wrench FOM PIE OA sone E2 SS Cap GO 343417A 4361453 418763A Tube vise E Box wrench F AL Cap 339083A 215786B 477456A RP21 322690A 80 Tool set F Tube vise F Torque wrench F2 AL Cap 339082A 215786A 477456A 325968A 80 Tool set F2 Tube vise F Torque wrench PASAT REA F SS Cap ZO 215786B 4361453 RP21 418759A Tube vise F Box wrench 63 Appendix 5 The list of bottles Poly
23. Hand Press S 40ALCap S410532A Tube Holder E P70AT P50AT2 Use the Tube Holder C inserted in Tube Holder E 60 Cap Tool Kit Cap Tube holder E Tube holder C Tube setter C Hand Press Ge 3 ei Ge Kits Ke Part No Name Qty Part No Name Qty Part No Name Qty PartNo Name Qty Part No Name Qty 5308626A S410542A 8 S411486 1 85411487 1 481056A 1 S204591A 1 S 12AL Cap Tool Kit S 12AL Cap Tube holder E Tube holder C Tube setter C Hand Press 5308626B S410542A 12 85411486 1 85411487 1 481056A 1 5204591A 1 S 12AL Cap Tool Kit S 12AL Cap Tube holder E Tube holder C Tube setter C Hand Press 5308627A S410532A a S411486 1 a 481056A 1 S204591A 1 S 40AL Cap Tool Kit S 40AL Cap Tube holder E Tube setter C Hand Press 5308627B S410532A 12 85411486 1 ZA 481056A 1 5204591A 1 S 40AL Cap Tool Kit S 40AL Cap Tube holder E Tube setter C Hand Press 61 Appendix 3 The list of space caps Select the correct space caps to match the tubes and rotors to be used Applicable tubes Name color Applicable rotors Old models 2PA Seal tube 5PA Seal tube 5402631 488101 Space cap A blue RP70VT B Space cap white RPV65T P100VT P65VT2 P9ONT P65NT2 P83VT P65VF P55VF2 RP65AF SRP83VT RP65VF RP55VF2 RP65VT2 RP67VF RP85NT B2 Space cap black 5403331 B3 Space cap white RP50AT4
24. INSTRUCTION MANUAL HITACHI ROTORS TUBES BOTTLES AND CAPS FOR Hitachi Preparative Ultracentrifuge This manual contains instruction for operation of Angle rotor Neo angle rotor Swing rotor and Vertical rotor Carefully read this manual in conjunction with manual for centrifuge and use the rotor correctly Retain it for future reference after reading 2014 12 599920413 Hitachi Koki O The contents in this manual are subject to change for improvement Copyright 2013 Hitachi Koki Co Ltd All rights reserved No part of this document may be reproduced or transmitted in any form or any means without permission from Hitachi Koki Co Ltd The names of actual companies and products mentioned herein may be the trademarks of their respective owners ABOUT Z MARKS In this manual Z N marks are used to make attention to prevent personal injury or machine damage The meaning of the mark is as follows ZX WARNING Indicates a potentially hazardous situation which if not avoided could result in severe personal injury or possible death A CAUTION Indicates a hazardous situation which if not avoided could result in personal injury or severe damage to the instrument SAFETY REMINDER Centrifuge rotors rotating at high speed have considerable potential for damage to personal properties if used improperly For safe and proper use of this rotor carefully read the centrifuge instruction manual and this rotor instruction manu
25. If using the rotor which handle separate into the handle and the handle bar be sure to remove the handle bar after setting the rotor 2 For operation refer to the instruction manual of the preparative ultracentrifuge Drive shaft 5 Removal of sample 1 Upon completion of centrifuge carefully remove the rotor from the drive shaft 2 Loosen the handle and remove the cover 3 Take tubes or bottles out with the tube setter etc When using the seal tube under 10 C it may be hard to take the tube out as soon as the rotor has stopped In this case take the tube out after 10 30 minutes 4 Recover supernatants or pellets ZX CAUTION Each time the rotor with optical adapter is used be sure to enter the results in the rotor log book Failure to do so will disable management of the life of rotor No entry is needed for the rotor with RLM adapter Perform maintenance and inspection of the rotor each time it is used If there is any abnormality immediately stop using the rotor and contact a Hitachi Koki authorized sales service representative Refer to 4 2 Maintenance of Rotors and 4 4 Inspection of Rotors 39 3 5 Swing rotors Swing rotors hook type buckets or pin type buckets other than the P32ST rotor I Preparation 2 Prepare the tubes 1 Chea ibe rator See 2 8 How to use Tube and Bottle Is a packing damaged or e gt Are the threads worn deteriorated 3 Prepare accessorie
26. P TEN f Pin RY He ne Bucket VW Rotor pin jody body Balance weight Pin guide Bucket Optical adapter disk Ge or RLM adapter Drive hole rrara type bucket Pires hule type bucket Some rotors do not have a rotor pin and a balance weight An old rotor may have a pin guide in place of a balance weight P32ST rotor top loading type buckets Rotor body Groove for the bar of the bucket g7 Cap D EEE O ring Bar Bucket body Bucket N P328D Only RLM adapter Cop bara ps Bec loading type bucket Fig 1 3 Names of parts of swing rotor Table 1 3 Specification of swing rotor Maximum Maximum x Rotor Rotor EE Speed RCF dE mL Ge capacity Ke body dai rom x 9 mL material P65ST 65 000 419 000 48 5x3 15 43 Titanium alloy Titanium alloy P56ST 56 000 409 000 54 4x6 24 5 2 Titanium alloy Titanium alloy P55ST2 55 000 366 000 50 5x6 30 55 Titaniumalloy Titanium alloy P40ST 40 000 284 000 139 13 x6 78 6 9 Titaniumalloy Titanium alloy P32ST 32 000 180 000 198 40 x6 240 7 1 Titanium alloy Titanium alloy P28S 28 000 141 000 252 40 x6 240 6 2 Aluminum alloy Titanium alloy Nominal capacity 1 5 Vertical rotors Vertical rotors are made from titanium alloy Table 1 4 shows the specifications of vertical rotors and Fig 1 4 shows the name of parts Maximum Optical adapter disk or RLM adapter Drive hole Fig 1 4 Names of parts of vertical rotor Table 1 4 Specification o
27. PA Tube E TI2 Cap Tube Vise E Tube Setter 5304987B 329607A 305231A 5401787A 343417A 435823A 40PA Tube Kit B 40PA Tube E3 AL Cap Tube Vise E Tube Setter 5304988A 5304299A 325968A S401792A 215786A 435823A 94PA Tube Kit 94PA Tube F2 AL Cap Tube Vise F Tube Setter ov Qy pcs box 477705 1 477705 Torque Wrench isss 1 2 477705 Torque Wrench 1 477706 477706 Socket Adapter 477706 Socket Adapter 1 berriri 483537A 19MM Socket 483539A 17MM Sockets rr Tr 4835374 or 483539A eSeal Tube Kit SEAL TUBE SPACE CAP TUBE SETTER The tube sealer model STF 1 or Parts model STF2 model STF3 and the tube rack are separately necessary for using Kits seal tubes See appendix 3 for the art No of the tube rack Part No Name PartNo Name PartNo Name E p S304989A 345319A 2 488101 a S407157 1 5PA Seal Tube Kit A SPA Seal Tube 100 B2 Spase Cap Tube Setter S304989A 345319A 2 488101 18 58407157 1 5PA Seal Tube Kit B 5PA Seal Tube 100 B2 Spase Cap Tube Setter S304990A 5304238A 2 5407828 a S407157 1 6 5PA Seal Tube Kit 6 5PA Seal Tube 100 B5 Spase Cap Tube Setter S304991A 345320A 2 486582 12 S407157A 1 12PA Seal Tube Kit A 12PA Seal Tube 100 C2 Spase Cap Tube Setter 5304991B 345320A 2 484285 12 58407157 1 12PA Seal Tube Kit B 12PA Seal Tube 100 C Spase Cap Tube Setter 304991C 345320A 2 402310 10 S407157 1 12PA Seal Tube Kit C 12PA Seal Tube 100 C4 Spase Cap Tube Setter 304992A 345321A 2 485649
28. To replace the optical disk follow the procedure below ZA CAUTION amp The optical disk plays an important role to control the rotor revolution speed The optical disk must match the maximum speed of the rotor e When replacing be very careful not to damage the optical disk and rotor body 51 e Replacing the optical disk 1 Preparation Prepare a new optical disk and knife lt optical disk gt Make sure that the number of stripes of the new optical disk is right as meted in Table 4 2 disk Wash and then dry the rotor well GO Optical Rotor l lt viewed from bottom gt Fig 4 3 Optical disk Table 4 2 Rotor s maximum speed and optical disk s number of stripes Rotor s maximum Stai Rotor s maximum ERE Rotor s maximum a speed pm bands speed en of black bands EEE ENE of black bands 100 000 9 50 000 18 30 000 29 90 000 10 48 000 19 28 000 32 80 000 83 000 11 45 000 20 27 000 33 70 000 13 42 000 21 25 000 35 65 000 14 40 000 22 23 000 39 60 000 15 38 000 24 21 000 40 55 000 56 000 16 35 000 25 19 000 45 54 000 17 32 000 28 17 000 50 2 Hold the rotor upside down 3 Pry the edge of the optical disk with the knife and remove the disk Be very careful not to damage the rotor 4 Clean the disk hole on the rotor by using alcohol 5 Remove the backing paper from the new optical disk Place the optical disk so that it snugly fits into the g
29. Upon completion of centrifuging carefully remove the rotor from the drive shaft 2 Remove the cap with the handle or the torque wrench 3 4 3 Take space caps and tubes out with the tube setter 4 Recover supernatant or pellets Z N CAUTION amp Each time the rotor with optical adapter is used be sure to enter the results in the rotor log book Failure to do so will disable management of the life of rotor No entry is needed for the rotor with RLM adapter e Perform maintenance and inspection of the rotor each time it is used If there is any abnormality immediately stop using the rotor and contact a Hitachi Koki authorized sales service representative Refer to 4 2 Maintenance of Rotors and 4 4 Inspection of Rotors 47 4 Maintenance 4 1 Anti corrosion property The anti corrosion properties depend on the material of the rotor See chemical resistance chart in separate manual The over speed adapters of all rotors are made from aluminum alloy The covers of some titanium angle rotors are made from aluminum alloy Consult Fig 1 1 The caps of all titanium vertical rotors are made from aluminum alloy The surface of aluminum rotors are anodized However when salt solution cesium chloride is used in a swing rotor s bucket made from aluminum alloy the bucket may be broken during centrifuging it it is corroded For salt solution use a rotor with a bucket made from titanium alloy or rep
30. WX CP MX and CP a B or STF3 series centrifuges this is not Tube Sealer required for balancing 7 Filling the sample Fill the samples of the same density into the f BO Small quantity tubes with an injector so a small quantity of of bubbles i Seal tube bubbles remains If the volume of the sample is small or the tubes are not welded completely leakage will occur from the shoulder part or the tube may be deformed 2 Balancing Put the bottle and the cap in the balance keep the difference of any two bottles which are arranged symmetrically within 0 3g When using CP NX CP WX CP MX and CP a B series centrifuges you can balance the tubes simply by leveling the sample within 5mm 3 Sealing the tubes Wipe off moisture 1 Wipe off moisture adhering inside the opening of the seal tube 2 Weld the seal tube according to the instruction manual of the STF 1 STF2 or STF3 tube sealer i Move the tube rack to the direction of arrows shown in the right figure to adjust position of the neck of the tubes under the heater Depress the handle until the heater presses the neck of the tube and then keep this condition for 2 to 3 seconds Then depress the handle to the end and wait until the HEAT lamp is put out Remove the protrusion on the welding part with your nail Push the seal tube slightly and check that the sample does not leak If the tubes are not welded completely the tube may be deforme
31. X OJ O O O0 OO O0 O0 O O XI OJO Chemical solution Hydrogen peroxide 3 x O 0 O0 0 O OJ X OJ O O 0 0 OJ O 0 GK GI O GO O OL GK OJ x Olx GOGOR GK GO DO O O O O O x x Formalin Green screw caps of bottles old type cannot be autoclaved Blue one can be autoclaved 14 2 5 Storage Store the tubes and caps at room temperature Do not store at high temperature or humidity or in a chemical vapor or expose to UV radiation 2 6 Inspection The cap space cap and O ring are consumable supplies their lives vary in different conditions of use Inspect them each time they are used and replace them if you find any damage deterioration or wear CAPS E Is the hex hole of the setscrew worn O Are the threads of the nut worn or corroded Is the crown corroded or cracked Is the weight ring deformed or corroded Is the O ring damaged Are the threads of the stem worn Is the stem deformed or corroded When the insert is worn contact the sales agent You can see the black insert at the reverse side of the stem OD Om Crack Do not use the cracked tube SPACE CAPS and CROWNS for re seal tubes and cone top tubes Is there a crack at bottom of the space cap or the crown Do not use the cracked space cap or crown Plugs for re seal tubes and cone top tubes Is the hex hole wom Are the threads worn or corroded
32. al before use and observe the instructions N WARNING Never use any sample capable of producing flammable or explosive vapors Your ultracentrifuge and rotor have no explosion proof construction d Your ultracentrifuge and rotor are not designed to confine any sample particles dispersed due to a leakage Therefore when using toxic or radioactive samples or pathogenic or infectious blood samples take additional precautions to prevent exposure to these materials e g use of isolated areas d Never exceed the maximum speed of the rotor stamped on it Exceeding the specified speed could break the rotor resulting in damage to the ultracentrifuge Check the chemical resistance chart attached the rotor and do not use any sample inapplicable to the rotor including buckets Using such a sample could corrode the rotor including buckets d Do not remove the RLM adapter or optical adapter from rotor or replace it with the adapter of another rotor The adapter is a critical component that detects the over speed of rotor If an adapter that is not compatible with the rotor is attached the rotor could break resulting in damage to the ultracentrifuge d Do not allow the rotor temperature to rise above 100 C This would cause the material to become brittle d Never autoclave the rotor or sterilize it in boiling water The strength of rotor may be significantly lost When using swing rotor be sure to set all buckets of the same type whether o
33. clean the rotor Using other detergents could discolor or corrode aluminum alloy portion of RLM adapter optical adapter etc e Clean the inside of the drive hole crown hole of the rotor and the surface of the drive shaft crown of the centrifuge once a month If the drive hole or the drive shaft is stained or any foreign matter is adhered the rotor may be improperly installed and come of during operation Hold the cloth to a cut Cleaning bar Fig 4 2 How to the use a cleaning bar 49 4 3 Sterilizing rotors Sterilize the rotor appropriately following Table 4 1 ZX WARNING Do not autoclave the rotor or sterilize it in boiling water because its material may deteriorate and its strength may decrease Table 4 1 Sterilizing of rotor O Usable x Not usable Aluminum rotors Titanium rotors 115 C 0 7kg cm for 30 min x x Autoclaving 121 1 Okg cm for 20 min x x 126 C 1 4kg cm for 15 min x x Boiling 15 30 min x x Ultraviolet rays 200 300 nm O O Ethylene oxide O O Gas Formaldehyde O O A Ethanol 70 O O Chemical l Hydrogen peroxide 3 O O solution 3 Formalin O O Heat resistance C 100 100 Do not dip the rotor in the formalin 3 solution more than 2 hours 4 4 Inspection of rotors Check the rotor as follows after every use If the optical adapter disk is unclean wipe it with a soft cloth but do not damage it Replacements o
34. d and the samples will leak Protrusion Welding part 29 e Thick walled tubes for angle rotors 30 Preparation b e Thick walled lt Sample gt lt Injector gt lt Balance gt tube When you use CP NX CP WX CP MX and CP o B series centrifuges this is not required for balancing J Filling the sample Fill the tube with the sample You can use the thick walled tube with optional volume less than net capacity Net capacity vary with using rotor Balancing Put the tube in the balance Keep the difference of any two tubes which are arranged symmetrically with in 0 3g When using CP NX CP WX CP MX and CP a B series centrifuges you can balance the tubes simply by leveling the sample within 5mm ZX CAUTION When using a 4 7PC thick walled tube at the speed of over 60 000 rpm in the P100AT P100AT2 rotor cap a 4 7PC thick walled tube with B TI lid Part No 5408523 e Tubes for swing rotors Preparation be lt Sample gt lt Injector gt lt Balance gt When you use CP NX CP WX CP MX and CP o B series centrifuges this is not required for balancing 7 Filling the sample Fill the tube up to within 3mm from the top of Within 3mm the tube with the sample 2 Balancing Put the tube in the balance keep the differen6e of any two tubes which are arranged symmetrically within 0 2g When using CP NX CP WX CP MX and CP a B series centrifuges you can bala
35. e Use the tubes bottles at a temperature between 4 C and 20 C PP Polypropylene Strong but susceptible to low temperature brittle point 0 C It can be autoclaved at 121 C or less Use the tubes bottles at a temperature between 4 C and 25 C PA Polypropylene copolymer Strong and can be autoclaved at 115 C or less Use the tubes bottles at a temperature between 4 C and 25 C Use a 30PA bottle C and 70PA bottle C at a temperature between 4 C and 20 C SS Stainless steel Excellent in chemical resistance and heat resistance It can be autoclaved at 121 C or less For chemical resistance see chemical resistance chart in separate manual 2 3 Washing Refer to table 2 1 for washing tubes and bottles 1 Wash with tap water or dilute neutral detergent after use If there is a heavy stain soak in dilute neutral detergent for a while or wash with a soft brush carefully 2 Rinse with tap water and distilled water 3 Dry in the air The PC tubes and bottles are inferior in chemical resistance with respect to alkali solution Do not use detergent of pH9 or above And do not allow the soaking in dilute detergent for a long time because otherwise the material would become brittle For caps take to pieces and wash in the same way with tubes After washing dry completely and store caps as a unit Take care of pH of detergent when performing ultrasonic washing 13 Table 2 1 Washing Conditions O Usable x Not
36. es service representative In such cases copy the attached decontamination sheet and fill out the copied sheet then attach it to the item to be returned Hitachi Koki may ask you about the treatment for the rotor or parts if the decontamination is checked and judged as insufficient by Hitachi Koki It is your responsibility to bear the cost of sterilization or decontamination If you have any question please send e mail to himac hitachi koki co jp Note that Hitachi Koki cannot repair or inspect the rotor or the accessory unless sterilization or decontamination is completed Rotor retirement After many years of use there will be inevitably some corrosion or stress corrosion At some points the combination of such damage and metal fatigue could make the rotor vulnerable to a failure Although a rotor may appear to be in a good condition you should follow the rotor retirement recommendation shown below Rotor Material Retire After Years Angle rotor Titanium alloy 12 Vertical rotor Aluminum alloy 10 Titanium alloy Swing rotoi Aluminum alloy ba Zonal rotor Titanium alloy 10 Continuous flow rotor Titanium alloy 10 69 Hitachi Koki Co Ltd 1060 Takeda Hitachinaka City Ibaraki Pref 312 8502 Japan Tel 81 29 276 791 4 Dial in Fax 81 29 276 7475 URL http www hitachi koki com himac
37. f packing and O rings are available If the rotor appears to be corroded deformation cracks or discoloration is observed do not use the rotor and call a Hitachi Koki authorized sales representative The rotor may be irreparable depending on the degree of corrosion and wear Angle rotors Are the threads worn Is the packing damaged or deteriorated Is the tube cavity corroded Is the adapter disk stained Is the balance weight loosened Is foreign matter adhering to the drive hole Is the rotor pin bent 50 e Vertical rotors and Neo angle rotors Are the threads worn Is the O ring damaged or deteriorated Is the adapter disk stained Is foreign matter adhering to the drive hole Is the balance weight loosened Is the adapter disk stained Is foreign matter adhering to the drive hole Is the rotor pin bent amp Buckets of swing rotors Are the threads worn Is the packing damaged or deteriorated Is the tube cavity corroded or cracked ZA CAUTION Check the packing O ring every time Replace it with new one if deterioration crack deformation etc is observed Otherwise samples might be damaged due to poor rotor seal or the centrifuge or the rotor might be damaged during operation 4 5 Replacing optical adapter disk The optical adapter disk if corroded or discolored must be replaced immediately Call the Sales Agent if it is necessary to replace the optical adapter
38. f vertical rotor Maximum Rotor Rotor K Tube Weight Cap or adapter ZERK d GO factor mL x Number Seta kg d material P100VT 100 000 700 000 6 5x 8 40 4 1 Titanium alloy Aluminum alloy P65VT3 65 000 402 000 13 12x10 120 10 1 Titanium alloy Aluminum alloy P65VT2 65 000 416 000 10 5x16 80 95 Titanium alloy Aluminum alloy P50VT2 50 000 243 000 36 40x 8 320 11 9 Titanium alloy Aluminum alloy Nominal capacity 1 6 Prevention of over speed If the rotor speed exceeds the maximum limit the rotor might break leading to a serious accident The RLM adapter or optical adapter disk fitted at the bottom of the rotor prevents overspeed of the rotor N WARNING Do not remove the RLM adapter or optical adapter from rotor or replace it with the adapter for another rotor The adapter is a critical component that detects the over speed of rotor If an adapter that is not compatible with the rotor is attached the rotor could break resulting in damage to the ultracentrifuge Z CAUTION Do not bring the RLM adapter near a magnet or scratch it Doing so will erase the memory stored in adapter and make the rotor unusable To prevent the adapter from being scratched store the rotor with RLM adapter using the stand provided with the rotor rotor stand for protecting adapter If the adapter is scratched immediately stop using the rotor and contact a Hitachi Koki authorized sa
39. he SG Stem fit on the mouth of the tube en ee 24 3 Depressing the Ti Ring Remove the tube with the Al Stem from the Tube Holder Tube Then mount the Ti Ring on the tube Se from the bottom of the tube with the groove of the ring facing downward Ti Ring ey e Se Ti Ring The groove of the ring should be set downward Set the tube as the above to the Tube Holder JEG Tube Holder stag Place the Tube Holder on the suitable position of the Hand Press Then push down the Handle until the Ti Ring contacts the step of the Al Stem Handle xX Depressing improperly Al Stem ay d Gap b P vr Tube Holder Ti Ring O Depressing properly i d Hand Press Tzi Although there may be about 0 5mm gap between the E Al Stem and the Ti Ring there is no problem in performance for the tube seal 25 4 Balancing Fill up the tube with the sample by Tighten the setscrew securely with using the injector etc the tube setter Otherwise the tube may be deformed Tube setter SO Use the pee wrench side Setscrew W Ze e during operation Tube Put the tube and setscrew in the Balance Keep the difference of any two tubes which are arranged symmetrically within 0 3g Tighten the setscrew to prevent the leakage of the sample When using CP NX CP WX CP MX and CP a B series centrifuges you can balance the tube
40. i Koki and failure or damage to a Hitachi Koki ultracentrifuge due to defects in a rotor covered by the warranty This warranty covers the rotor or ultracentrifuge only and Hitachi Koki shall not be liable for damage to samples or other items that may arise from failure of or damage to the rotor or ultracentrifuge 2 Warranty Period This warranty is valid for five years from the date of delivery of the rotor by Hitachi Koki or a Hitachi Koki dealer However except as provided under 1 below even within such period the warranty does not cover cases where the user has exceeded the applicable limit on the number of runs or the cumulative operating time for the type of rotor as set forth in 1 and 2 below 1 Aluminum alloy angle rotor swing rotor zonal rotor and continuous flow rotor O The limits on the maximum number of runs and the maximum cumulative operating time for these rotor types are stated in the table below The warranty covers the time up until either of these limits is reached provided that it is reached during the warranty period and that the maximum and allowable speeds are not exceeded In order for the warranty to cover use after either of the limits in the table below is reached inspection by Hitachi Koki will be necessary The user must pay for such inspection If Hitachi Koki s inspection finds that the rotor is free of corrosion and other abnormality Hitachi Koki will then further warrant the rotor up unti
41. ion equilibrium Neo angle rotors This rotor holds the tubes at a smaller angle to the axis of rotation than the angle rotors It is effective to separate samples that produce precipitates and floating matter such as plasmid DNA in a short time Swing rotors a This rotor holds tubes in the bucket and swings the bucket in the centrifugal direction It is mainly used for high precision separation such as to separate components in a cell using the density gradient sedimentation velocity Vertical rotors This rotor holds the tubes perpendicularly to the centrifugal force It is expected for separation in a shorter time with this rotor than with swing rotors and angle rotors It is specially effective to separate nucleic acid using the density gradient sedimentation equilibrium 2 Classified into materials Titanium rotors Rotors made from titanium alloy Aluminum rotors Rotors made from aluminum alloy 3 Classified into management type Rotor with optical adapter disk FC i The rotor s operation history is recorded in the log book for control An optical adapter disk is attached as an optical over speed prevention device RLM rotor eeen This is exclusively used with a CP NX a CP WX a CP MX or a CP a ultra centrifuge The rotor life management RLM system controls the rotor s operation history automatically An RLM adapter is attached for magnetic recording 1 2 Angle rotors Angle rotors are made from
42. l either of the limits in the table below is reached once more but with the rotor s maximum speed reduced by 10 Even in such case however the warranty period shall remain limited to five years from the date of delivery and shall not be extended Maximum number of runs Maximum cumulative operating time P32ST rotor 500 2 500 hours Rotors other than the P32ST rotor KE 2500 hoars 2 Titanium alloy angle rotor titanium alloy vertical rotor and neo angle rotor The limits on the maximum number of runs and the maximum cumulative operating time for these rotor types are stated in the table below The warranty covers the time up until either of these limits is reached provided that it is reached during the warranty period and that the maximum and allowable speeds are not exceeded With these types the warranty cannot be extended beyond the stated limits even if the rotor undergoes inspection by Hitachi Koki Maximum number of runs Maximum cumulative operating time 5 000 10 000 hours 67 3 Warranty Terms 1 Should a rotor covered by this warranty fail or be damaged Hitachi Koki will replace it with a new rotor in return for payment of a sum determined according to the formula below based on the ratio of the actual number of runs or cumulative operating time to the maximum number of runs or cumulative operating time Actual number Actual cumulative Replacement price Current price of rotor x
43. lace with a bucket made from titanium alloy Consult the sales agent when replacing the bucket 4 2 Maintenance of rotors Do maintenance the rotor to avoid corrosion after use 1 Ordinary maintenance 1 Wash the rotor with tap water or dilute neutral detergent 2 Rinse with distilled water 3 Wipe off water drops with soft cloth and dry the rotor up side down After drying coat the rotor with the silicone grease vacuum grease For drive hole coat with silicone grease vacuum grease and wipe it Store the rotor in a dry place Be sure to keep the RLM rotor on the rotor stand provided to protect the RLM adapter a Coat with the Vacuum grease b Coat with the silicone grease Vertical rotors Angle rotors Neo angle rotors Bucket Fig 4 1 Coating the grease 48 For the rotor body of the swing rotor wipe and coat with the silicone grease vacuum grease 2 After using corrosive samples Wash the rotor with flowing water for a while and then perform ordinary maintenance 3 If foreign matter adheres to the rotor Soak the rotor in warm water 40 50 for a while and remove foreign matter with a cleaning bar or a soft brush If you cannot remove it contact a Hitachi Koki authorized sales service representative ZX WARNING Do not allow the temperature of aluminum rotors and titanium rotors to rise above 100 This would cause the material to become brittle ZN CAUTION amp Use only detergent pH 5 9 to
44. les service representative Take great care not to scratch the optical adapter Any scratch on adapter will make the rotor unusable To prevent the adapter from being scratched store the rotor with optical adapter using the stand provided with the rotor rotor stand for protecting adapter If the adapter is scratched immediately stop using the rotor and contact a Hitachi Koki authorized sales service representative SI foreign matter adheres to the RLM adapter or optical adapter disk wipe it off promptly Otherwise the RLM adapter or optical adapter disk may be corroded The RLM adapter of RLM Rotor is a magnetic memory Use care to protect it from damage and keep it away from magnetism otherwise memory contents would be destroyed To protect the RLM adapter be sure to keep the RLM Rotor on the rotor stand provided The optical adapter disk of the standard rotor has stripes of black not reflecting light and white reflecting light as shown in Fig 1 5 and the number of stripes depends on the maximum speed of the rotor This adapter disk keeps the rotor speed under the maximum permissible value See 4 5 Replacing Optical Adapter Disk for replacing the optical disk Optical Optical adapter disk Fig 1 5 Optical adapter disk At model 55PA 65P and 55P 2 if over speeding occurs a small pin jumps out from the pin guide and the overspeed preventing system functions to reduce speed When using an old type ult
45. load to the rotor which is very dangerous The crystallization will also greatly affect the density gradient of the CsCl solution and separation status of the sample Therefore always use a solution which will not crystallize within the allowable speed 36 3 Allowable speed for the combination of tubes caps etc The speed of the rotor should be limited by the combination of tubes caps etc For detail consult Rotors Adapters Tubes Bottles and Caps accessories of centrifuges 4 Allowable speed for the life of aluminum rotors The aluminum rotors should be limited the speed after 1 000 runs or 2 500 hours used See 4 6 Life of rotors 3 2 Sample limitation N WARNING Never use any sample capable of producing flammable or explosive vapors Your ultracentrifuge and rotor don t have an explosion proof construction Your ultracentrifuge and rotor are not designed to confine any sample particles dispersed due to a leakage Therefore when using radioactive toxic or pathogenic materials take additional precautions to prevent exposure to these materials e g use of isolated areas 3 3 Adhesion of sample etc A CAUTION If sample etc adheres to the rotor use a soft cloth etc to wipe it off leaving it could corrode the rotor Especially if sample adheres to the RLM adapter or optical adapter of rotor immediately wipe it off The adapter is aluminum alloy and susceptible to co
46. loy and plugs are made from stainless steel Be different rotors use different crowns or plugs OAdapters Adapters are used when relatively smaller tubes are inserted into the tube cavities Most adapters are made from white polyacetal Since the specific gravity of adapters is high the rotation speed is restricted when adapters are used Fig 2 2 Bottles Ele Set screw Nut S Washer b Crown Weight ring p assembly C Orting or Gasket Some caps do not have the parts in brackets Fig 2 3 Caps Fig 2 4 Adapters 2 2 Materials of tubes N CAUTION Run the rotor at the proper temperature see the below Otherwise the tubes may be damaged or deformed during operation When running the rotor beyond this limit test the tube to ensure that there is no abnormality before actual operation ZA CAUTION Check the chemical resistance chart in separate manual and do not use any sample inapplicable to the tubes and the bottles PC Polycarbonate Strong transparent and can be autoclaved at 121 C or less Use the tubes bottles at a temperature between 4 C and 25 C Weak in organic solution alkali solution and alkali detergent PET Polyethylene terephthalate Transparent Can not be autoclaved Sliceable and puncturable Weak in organic solution Use the tubes bottles at a temperature between 4 C and 20 C PE Polyethylene Opaque Can not be autoclaved Excellent in chemical resistanc
47. m The speed rpm can also be calculated from the RCF x g using this formula 2 To find the separation time The sedimentation time T required for the particles scattered in the solution to lower from the top r to the bottom rp of the tube The sedimentation characteristic is expressed as sedimentation coefficient s but generally the sedimentation coefficient s becomes a very small value and s x 10 may be indicated as S This S expressed as s x 10 is called the sedimentation coefficient in Svedberg units However in biochemistry etc it may simply be called the sedimentation coefficient w Angular velocity w N Speed rpm ri Distance from axis of rotation to top of solution fp Distance from axis of rotation to bottom of tube In tp In r 10 3 wW 3600 Here if it is defined that K T hr If S is unknown the following formula Stokes law is used for calculation dipu Pi 13 _ x 1 S 18n 0 d Diameter of lowering particle cm p Density of solution surrounding particles g mL p2 Density of lowering particle g mL n Viscosity of solution surrounding particles Poise The viscosity of water at 20 C is 0 01 Poise S in the pure water at 20 C is expressed as Szo w K is called the K factor clearing factor which varies depending of the type of rotor and the speed When the rotor is used at a certain speed the K factor Ky can be obtained from the formula shown
48. m the bucket 4 Take tubes out with tweezers 5 Recover supernatants or pellets Z CAUTION Perform maintenance and inspection of the rotor each time it is used If there is any abnormality immediately stop using the rotor and contact a Hitachi Koki authorized sales service representative Refer to 4 2 Maintenance of Rotors and 4 4 Inspection of Rotors 44 3 6 Neo angle rotors and titanium vertical rotors Preparation Ghee We vale 3 Prepare the accessories Is the O ring i EO i Space cap for seal tubes damaged or i matters in the tube cavities Tube setter for seal tubes X Tube setter C for tubes d re seal tubes or cone top tubes Seal tubes Re seal tubes and cone top tubes Tightening tool Setting the tubes 1 Load the rotor symmetrically with the tubes within allowable imbalance 0 3g or less by using the tube setter the removal tool or the tube setter c Excessive imbalance may damage the rotary shaft of the centrifuge Tube setter Tube setter C r E Use the screw o Removal tool SG side l Tube Rotor Sealtube gt Cone top tube Space cap d prevent deformation of the tubes during operation A Seal tube TISS Re seal tube 2 Place the space caps on the tubes to 45 46 3 Setting the caps to rotor Using SEAL TUBE in P100VT P83VT P65VT2 1 Fix the holder base to a desk etc Torque wrench 2 Fix
49. nce the tubes simply by leveling the sample When using three tubes keep the difference of three tubes within 0 2g 31 e Re seal tubes and cone top tubes Preparation I A lt Tube gt lt Crown and Plug gt lt Tube setter C gt Tools to tighten the caps lt Balance gt When you use CP NX CP WX CP MX and CP o B series centrifuges this is not required for balancing lt Injector gt lt Sample gt 7 Preparing the cap 1 Fix the tube vice to a desk etc by turning the handle B 2 Confirm the combination with the tube and the Crown assembly Beart Handle A Handle B 2 Filling the sample Fill the samples of the same density into the tubes with an injector so a small Small quantity b of bubbles quantity of bubbles remains If the volume of the sample is small or the tubes are not welded completely leakage will occur from the shoulder part or the tube may be deformed 32 4 Attaching the crown and the plug to the tube Balancing Put the tube in the balance Keep the difference of any two tubes which are arranged symmetrically with in 0 3g When using CP NX CP WX CP MX and CP a B series centrifuges you can balance the tubes simply by leveling the sample within 5mm 1 Insert the crown into the top of the tube Push the crown to the top of the tube and the bottom of the crown contact closely 2 Set the assembly of the tube and c
50. ng imbalance during the operation different caps different tubes different density Fig 2 7 Correct balancing 35 3 How to use rotors 3 1 Allowable rotor speeds The rotor should never be used at any speed higher than the maximum speed marked on the rotor in any 6ase The rotor speed is limited by the following conditions DO NOT exceed this allowable speed 1 Allowable speed for density of the sample To centrifuge a sample of an average density more than 1 2g mL Determine the allowable speed from the following equation Allowable speed rom maximum speed rpm x a e Average density of a sample g mL To centrifuge a sample with seal tubes in neo angle rotors or vertical rotors These rotors can be used at their maximum speed with a sample with an average density less than 1 7g mL But in case of centrifuging a sample of an average density more than 1 7g mL in these rotors determine the allowable speed from the following equation Allowable speed rom maximum speed rpm x pear e eo Average density of a sample g mL 2 Allowable speed for density gradient medium The cesium chloride CsCl solution is frequently used as a density gradient medium but if the CsCl solution with a high density is used it may saturate during the rotation of the rotor depending on the rotation condition which may cause CsCl crystals to be educed Educed crystals have a high density approx 4g mL and apply an excessive
51. of runs or operating time whichever is greater Max number Max cumulative of runs operating time Replacement price Current price of drive unit x 2 Should an ultracentrifuge fail or be damaged due to failure of or damage to a rotor covered by this warranty Hitachi Koki will supply free of charge all parts required for repair of the ultracentrifuge However in the case of failure of or damage to an ultracentrifuge s drive unit Hitachi Koki will replace it with a new drive unit in return for payment of a sum derived by multiplying the current price of the drive unit by the ratio of the actual total number of revolutions or years completed to the warranted total number of revolutions or warranted years that are stated in the operator s manual for the ultracentrifuge as in the formula below Actual number of i Years completed revolutions or EIE competed Whichever is greater Warranted total Warranted years number of revolutions 4 Notes 1 This warranty extends only to the original buyer from Hitachi Koki or the original buyer from a Hitachi Koki dealer Rotors whose ownership passes from the original buyer to a third person due to resale transfer or the like are not covered by this warranty Neither does this warranty cover cases where the ownership of a rotor is reacquired subsequently by the original buyer 2 The warranty for the rotor proper warranty period and terms shall continue to apply in cases where
52. of this manual N CAUTION Each time the rotor with optical adapter is used be sure to enter the results in the rotor log book Failure to do so will disable the management of the life of rotor No entry is needed for the rotor with RLM adapter m When using the rotor with optical adapter the rotor log book is essential to manage the life of rotor and for warranty when an accident occurs Be sure to enter the results in it when using the rotor with optical adapter and do not lose it If any results of use are not entered in the rotor log book note that the rotor will not be covered by warranty 5 Troubleshooting 5 1 Rotors Check the rotor immediately if it has something wrong Table 5 1 summarizes trouble symptoms possible causes and actions to be taken e If the rotor has fallen on the floor by accident have it checked by a Hitachi Koki authorized sales service representative whether of not it is deformed If the rotor has fallen in the rotor compartment of the centrifuge do not use the centrifuge and call a Hitachi Koki authorized sales service representative immediately e If there is only a trifle symptom of trouble locate and remove the cause If the cause cannot be located do not use the device and call a Hitachi Koki authorized sales service representative immediately Table 5 1 Troubleshooting of the rotor Symptom Possible causes and actions The rotor packing or cap Are handle
53. ors and vertical rotors Preparation lt Tube gt lt Cap gt lt Tube setter B E Tightening wea lt Balance gt of the caps When you use See appendix 4 CP NX CP WX CP MX and CP o B series centrifuges this is not required for balancing lt Injector gt lt Sample gt 1 Preparing the cap 1 Confirm the composition of the cap 2 Fix the tube vice to a desk etc by turning the handle B Set screw Geek Nut 0 Py washer Crown weight ring Turn the flat surface of the weight ring to the upper when assembling Desk the cap lt Gasket E Handle A l O ring 0 Handle B Stem G 17 2 Assembling the cap 1 Assemble the cap loosely Unscrew the setscrew 2 Fill the tube upto three fourths with Remove the set screw L of the cap using hex the sample depress the cap until wrench side of the tube A the tube comes in contact with the AS Setter crown 3 Tighten the nut manually 3 Tighten the Cap 1 Insert the tube and the cap into the Nut Crown hole of the tube vice If there is a step between the top surface of the crown and that of tube Stem vise depress the cap until there is no step between them 2 Tighten the handle A of the tube vise to fix the tube and cap When you use the tube vise with the stopper t
54. ors have the balance weight In case of using the these rotors with the former centrifuge model 55PA 55P 2 or 65P install the pin guide to the rotor in place of the balance weight In case the pin guide is necessary please buy it DO NOT use rotor without pin guide with these centrifuge because it causes danger Note5 The P32ST rotor is a RLM rotor The P32ST rotor is not a rotor with an optical adapter disk The P32ST rotor can be used only in a series of CP NX WX MX ultracentrifuges P55AT RP55T SRPSOAT RP50T P50A2 RP50 2 P42AT RPL427 P42A RP42 RP40 2 RP40 RP30 3 P30A2 RP30 2 RP21 P19A RP19 SRP28SA SRP28SA1 RPW50T Pin guide Balance weight RPW45 RPW35 P48ZT RPZ48T i Rae e P35ZT RPZ35T P32CT RPC32T daaa eOld rotors with black adapter are used with model 55PA 55P 2 or 65P only Old rotors which have no adapter are used with model 40 P or 55P only Fig 1 8 Black adapter No adapter 1 8 Calculation of separation characteristics 1 Relationship between the relative centrifugal force and speed The relative centrifugal force RCF generated by rotation is related to the speed and radial distance from the axis of rotation The RCF is obtained from the following formula Generally the RCF is expressed as a ratio of the earth s gravitational acceleration and x g is used as the unit RCF 1 118 x 10 r x N x 9 N Speed rpm r Radial distance from the axis of rotation c
55. out how to inspect the cap refer to Holder according to the kinds of tubes the instruction manual attached to the centrifuge or the rotor D Setscrew Al Stem NY Insert is inside Stem Tube Holder C Tube Holder D Tube Holder E Check that NY Insert is not For 12mL Tube For30mLTube For 40 mL Tube Use the Tube Holder C inserted in Tube Holder E Ti Ring Set it for the correct direction The groove of the ring should be set downward Tube Holder C is used with Tube Holder E during operation 23 2 Depressing the Al Stem Remove the setscrew from the Remove the setscrew from the Al Stem cap using hexagon wrench side of the tube setter Setscrew Fill the tube up to three fourths R Al Stem with the sample Place the Al Stem on the mouth Ea of the tube GISet the tube in the Tube Holder SAA Place the Tube Holder on the suitable position of the Hand Press Then push down the Handle to depress the Al Stem to the desirable position Handle Depressing improperly TE The step of the Stem is inside the Aube koder tube A TJ I ar Al Stem Sa ZKO There is a gap between the step of the Stem and the mouth of the tube TT Tube Depressing excessively Depressing insufficiently Hand Press O Depressing properly oe Depress the Al Stem until the step of t
56. propylene copolymer How to indicate Parts of bottles Part No and material e The upper number is part No The parts with d mark are 1 po set and others are 10 pcs set If blank the part is not provided A e The lower letters indicate materials Material PA polypropylene copolymer PPO polyphenylene oxide PP polypropylene AL aluminum alloy Screwcap Innercap O ring Bottle PC polycarbonate POM polyacetal NBR nitrile butadiene rubber lt Bottles gt Parts of bottles Part No and material Part No Name Applied rotors old models Screw cap Inner cap O ring P90AT P80AT P70AT2 P65AT P55AT SRP70AT RP65 P90AT P80AT P70AT2 10 325952A 10pcs set 5401802A de be Sa P65AT P55AT SRP70AT 10PC Bottle PP RP65T P90AT P80AT P70AT2 P65AT P55AT SRP70AT 334105A 10pcs set S401802A 10PA Bottle PP 5303922A 6pcs set 474042A 474043A 474044A 336626A 10PC Bottle B PPO PPO NBR PC RP65T 330345A 10pcs set 8401789A geu db Te 30PA Bottle PP RP50 2 P70AT P50AT2 P50A2 5308133A 6pcs set 474035A 474036A 474037A 5308134A RP7OT RP50T 2 Sa 30PC Bottle B3 PPO PPO NBR PC RP50 2 P70AT P50AT2 P50A2 RP70T RP50T 2 RP50 2 5308132A 6pcs set 485541 474036A 474037A S308134A 30PC Bottle C3 AL PPO NBR PC S310626A 6pcs set 485541 S413364A 474037A 310625A 30PA Bottle C AL PP NBR PA 5308433A 6pcs set 474039A
57. r not samples are put in buckets Failure to do so could not only cause the rotor to vibrate but could result in the rotor being deformed and the buckets becoming detached which is very dangerous Never use buckets made by other companies or any other type of bucket that is not exclusively made for the rotor even if it is made by Hitachi Koki If the centrifuge rotor or an accessory is contaminated by samples that toxic or radioactive or blood samples that pathogenic or infectious be sure to decontaminate the item according to good laboratory procedures and methods If there is a possibility that the centrifuge rotor or an accessory is contaminated by samples that might impair human health for example samples that are toxic or radioactive or blood samples that are pathogenic or infectious it is your responsibility to sterilize or decontaminate the centrifuge rotor or the accessory properly before requesting repairs from a Hitachi Koki authorized sales service representative It is your responsibility to sterilize and or decontaminate the centrifuge rotor or parts properly before returning them to a Hitachi Koki authorized sales service representative Z N CAUTION Check the chemical resistance chart in separate manual and do not use any sample inapplicable to the tubes the bottles the tube adapters or the caps Using such a sample could deteriorate them The allowable speed may be lower depending on the density of sample use
58. racentrifuge refer to 1 7 Relation between Rotor and Ultracentrifuge Pin ae 1 7 Relation between rotors and ultracentrifuges The system to prevent the over speed is different from the system of the present centrifuge Therefore usable rotor is limited Use the proper rotor in accordance with Table 1 5 Table 1 5 The relation between Rotor and Ultracentrifuge EEEa Type of i CP90NX Centrifuge GPSONX CPWX Series 55P 3 80P 7Series 80P 7 etc GEG 72Series 85P 72 etc CP80WX SCP H Series SCP85H etc CP MX Series SCP H2 Series SCP85H2 etc Sede CP Series CP70 etc CP56G CP70MX CP B Series CP85 B etc CP a Series CP100a CP90H a CP80H a Note 1 Note 5 A RLM adapter Note 2 Note 3 Note 4 A Note 1 A Optical adapter disk O Usable A Refer to the notice x Impossible to use Note 1 Do not use a P21A2 rotor with the centrifuge other than a series of CP NX WX MX ultracentrifuges Note 2 Following rotors shall not be used with the former centrifuge model 55PA 55P 2 or 65P to use them safely P70AT RP70T PSOAT4 RP50AT4 PSOAT2 RP5OT 2 PASAT RP45T P65NT2 P65NT RP65NT RPV65T P65VT2 RP65VT2 P65VT3 RP65VT3 P50VT RPV50T RPV45T P50VT2 Note 3 Following rotors shall not be used with the former centrifuge model 55PA because the rotor chamber of model 55PA is small P1 9A RP19 PAOST RPS40T P28S SRP28SA P28S2 SRP28SA1 Note 4 All of following rot
59. roove of the disk hole on the rotor Make sure the disk does not move 52 e Pin Guide Replacement When used with the 55PA 65P and 55P 2 centrifuges some rotors may need a pin guide 1 7 Relation between Rotor and Ultracentrifuge If the rotors has a balance weight in place of a pin guide replace it with a pin guide If the pin protrudes from the pin guide the pin guide should be replaced Pin guide Balance weight Fig 4 4 Pin guide attachment positions and how to attach Use the spanner provided with the centrifuge to remove the pin guide or balance weight Attach the new pin guide and tighten it with the spanner securely e When replacing the pin guide check the number indicated on its head and use the guide the number of which corresponds to the maximum permissible speed of the rotor Example If the maximum speed of rotor is 50 000 rpm the number should be 50 The maximum permissible speed decreases with an aluminum alloy rotor that has exceeded its first lifetime Be careful when selecting a pin guide for such a rotor 53 4 6 Life of rotors 54 ZX WARNING amp Do not use the rotor that has exceed it s lifetime to do so might cause the rotor failure and the centrifuge might be damaged While using rotor repeatedly its strength decreases gradually due to fatigue and creep of material by which the rotor life is determined Swing rotors other than the P32ST rotor and Aluminum angle rotors The swing rotors
60. rotor parts and the like are additionally purchased In such cases the warranty period shall continue to be measured from the date of delivery of the rotor If such parts are subject to a restricted warranty period this warranty shall only cover failure or damage involving such parts that occurs both within the rotor warranty period and within the warranty period for the parts concerned 3 This warranty does not cover any of the following even during the warranty period Failure or damage due to abuse or misuse Failure or damage due to operation or maintenance in a manner contrary to the instructions in the operator s manuals for the rotor and ultracentrifuge Failure or damage due to disassembly or modification without the permission of Hitachi Koki Failure or damage due to use of a bucket adapter tube bottle or the like other than those approved by Hitachi Koki for use with the product Failure or damage due to disaster such as fire or earthquake Failure or damage due to use with an ultracentrifuge not of Hitachi Koki manufacture without the permission of Hitachi Koki Failure or damage due to use with an ultracentrifuge that has been modified without the permission of Hitachi Koki Failure or damage due to a failed or damaged part including a tube bottle adapter etc not submitted to Hitachi Koki Failure or damage not recorded in a rotor use log book in the case of an optical adapter type rotor
61. rown to the tube vice as shown in Fig Be sure that there is no difference in level between the surface R of the crown and the surface B of the tube vice If the surface A is higher than the surface B push the crown down to be no difference in level between A and B Tube vice Hold by hand 33 3 Fix the crown and the tube by tuming the handle A of the tube vice 4 Tighten the plug from the top of the tube using a tube setter C Normal assembly completion state is shown in Fig a To prevent the sample leakage during the operation it is important that the plug is securely fastened as shown in Fig a If the plug is not fastened securely as shown in Fig b fasten it tightly Even though the plug is slipped by hard fastening it can be used The plug must be securely fastened 1 ES r eS av EE Contact a CORRECT HOW TO REMOVE THE TUBES FROM THE ROTOR Draw out tubes from the rotor or the adapter with removal tool Before collecting samples set the assembly of the tube and the crown to the tube vice again And then remove the plug with the tube setter C When removing the crown from the tube after collecting the samples use the tube remover provided with the tube vice as shown in Figure Turn clockwise and depress the tube to remove Removal tool While holding the removal tool and the crown remove the tube by adding force in the arrow direction as shown in the figure J
62. rrosion If the RLM adapter or optical adapter corroded the rotor will be unusable 37 3 4 Angle rotors Preparation 1 Check the rotor 2 Prepare tubes or bottles See 2 8 How to use Tube and Bottle Are the threads worn Is the packings damaged or deteriorated Is there foreign matters in the tube cavities When using seal tube space cap is necessary See appendix 3 7 Setting the tube or the bottle 1 Set the tube or the bottle in the rotor with the tube setter or the bottle setter Tube setter Use the screw side 2 When using the seal tube Place the space cap on the tube to prevent deformation of the tube during operation C Space cap d ZA CAUTION Set the tube or the bottle Ze symmetrically within the DA Seal tube allowable imbalance 0 3g or a Ze less 38 3 Setting the cover Fit the cover to the rotor and tighten the handle Unless tightened adequately packings may come off during operation A CAUTION Be sure to attach the rotor cover to the rotor body and tighten handle securely Insufficient tightening of the handle can cause coming off the rotor cover and damage the centrifuge and the rotor amp If using the rotor which handle separate into the handle and the handle bar insert the handle bar to the hole of the handle Handle Handle bar ai Sa 1 Gently and securely set the rotor onto the drive shaft 4 Operation Z CAUTION
63. s Is there foreign matter _ Bucket stand in the tube cavities Tweezers Are the pins deformed E D tool 2 Preparation of the bucket 1 Remove the cap from the bucket 3 Tighten the cap of the bucket Stand the bucket in the bucket stand P65ST Set the supplied driver into the slot of the cap and tighten it completely Bucket Driver Bucket stand 2 Set the tube to the bucket Be sure to set balanced tubes symmetrically Bucket Tweezers Tube Bucket 40 P28S P2852 Tighten the numbered cap to the same numbered bucket by hand to match each mark of the bucket and the cap Tighten the cap until the bottom of the cap bumps to the bucket and the cap must be adjusted to the match mark of the bucket P55ST2 P50S2 P40ST P56ST Tighten the numbered cap to the same numbered bucket with the supplied cap spanner to match each mark of the bucket and the cap Tighten the cap until the bottom of the cap bumps to the bucket and the cap must be adjusted to the match mark of the bucket Mark Cap spanner Hook e Bucket body Bucket P55ST2 P50S2 P56ST P40ST N CAUTION Imperfect tightening of the cap may cause imbalance operation and it may cause the serious accident 3 Setting the bucket A WARNING When using swing rotor be sure to set all buckets whether or not samples are Put in buckets Failure to do so could not only cause the rotor to vibrate but could result in
64. s you can balance the tubes simply by leveling the sample 1 Fit the cap to the tube depressing 2 Tighten the nut of the cap with the the cap until the tube comes in box wrench contact with the crown Box wrench Cap 4 6 Tighten the Cap Turn the 1 Insert the tube and the cap into the hole of handle A the tube vice and fix them 2 Tighten the nut of the cap securely with the box wrench If tightening is not securely leakage will occur Box wrench 3 Remove the tube from the tube vice e Tubes 12PA 12PE 40PA 40PE tubes that are applicable for S Cap ee are applicable for PSOAT P80AT P70AT2 P70AT P50AT2 angle rotor For more details see di instruction manual Part No S999884 of S caps N CAUTION If there is any abnormality such as corrosion stop use of the S Cap and contact a Hitachi Koki authorized sales service representative NY Insert Part No S401791A are consumable parts It is necessary to replace it with the new one if it is wom Contact a Hitachi Koki authorized sales service representative when replacing it Preparation lt Tube gt lt Balance gt lt Injector gt When you use CP NX CP WX CP MX and Example CP o series centrifuges this is not required Injector whose diameter is 1 5 mm for balancing 7 Preparing the caps and adapters Confirm the composition of the cap 2 Select the S Cap Tool Set and Tube For ab
65. s simply by leveling the sample 26 e Bottles Preparation lt Injector gt lt Sample gt lt Balance gt When you use CP NX CP WX CP MX lt Bottle gt and CP a B series centrifuges this is not required for balancing EIO the sample Fill the bottle with the sample When a 30PC bottle C3 30PC bottle C l 30PA bottle C 70PC bottle B 70PC 4 Sebe line bottle C 70PA bottle C 80PC bottle C2 or 80PC bottle C are used at 100 000 x g or more max G Force the liquid should be over the shoulder of the bottle Any volume which does not exceed the net volume is applicable at less than 100 000xg Balancing Put the bottle and the cap in the balance keep the difference of any two bottles which are arranged symmetrically within 0 3g When using CP NX CP WX CP MX and CP a B series centrifuges you can balance the tubes simply by leveling the sample within 5mm NOTE Although you balanced the bottles within the approximate allowable imbalance 6mm alarm message IMBALANCE might appear Balance bottles more accurately again if the alarm message IMBALANCE appears see the rotor instruction manual 3 Tightening the cap Fit the cap to the bottle and tighten the cap securely by hand 27 eSeal tubes 28 Preparation GO lt Seal tube gt lt Sample gt lt Injector gt lt Balance gt lt Tube rack gt When you use CP NX at EE CP
66. st available for examination 57 Appendix 1 The list of caps for tubes Different caps use a different composition or parts materials Be careful not to combine unsuitable parts Component zo dudik dk 0 5 FD Setscrew Nut Washer Crown Weight ring E ring Stem Insert When seeing the stem from the bottom you can see the black insert e Materials EGISOTG rr SS stainless steel Washer EES ERES OEE CCE CREE erie POM polyacetal Crown AL aluminum alloy Ti titanium alloy Nut Pe TEE EEE EEE eee or SS stainless steel Stem See the list at next page Weight ring trer AL aluminum alloy or Ti titanium alloy See the list at next page Gasket NBR nitrile outadiene rubber or CR O ring chloroprene rubber See the list at next page Insert Eee ER EEE REE EEE ERGA EE EEK Nylon 58 Caps Nut Washer Crown Weight ring O ring Gasket Stem A2 AL Cap 336710A 80130235 AL 474117 S406640A NBR 474116 A3 AL Cap 336711A 80130058 5401807A NBR B AL Cap 414429A B2 AL Cap 474507A B TI Cap 460696A 5401829A SS 5401829A SS 5401829A SS 441393 AL 474641 5401821A NBR 5401778A CR S406626A NBR 453411A AL 474639A AL 460697A TI 5401791A NY 5401791A NY 5401791A NY B2 TI Cap 474070A 5401829A SS 4595612 Tl 474073 5401815A NBR 474071A TI 5401791A NY C AL Cap 441399A 5401829A SS 441393
67. the rotor being deformed and the buckets becoming detached which is very dangerous Never use buckets made by other companies or any other type of bucket that is not Match numbered exclusively made for the rotor even if it is made by buckets with number z i of rotor and set the Hitachi Koki buckets to the rotor Number All rotors except P65ST Hook Rotor Bucket Turning their flat Driver surface to the revolving axis 1 Insert the pin through holes of the rotor and the bucket Hang the hook of the bucket on the pin of the rotor When using the rotor which has two hooks make sure that both hooks hang on the pin 2 Tighten them with supplied driver 41 4 Operation ZX WARNING Before setting a rotor in ultracentrifuge make sure that buckets have been securely installed in the rotor Incorrect installation of buckets in rotor could damage the rotor or detach the buckets which is very dangerous eWhen using the rotor with hook type buckets be sure to hang on the bucket on the pin of the rotor correctly with a mirror or like this Carry the large rotors with the supplied rotor setter After setting the rotor be sure to remove the rotor setter 1 Gently and securely set the rotor onto the drive shaft 2 For operation refer to the instruction manual of the preparative ultracentrifuge 5 Removal of sample 1 Carefully remove the rotor from the drive shaft 2 Remove the b
68. the rotor to the ditch of the holder base 3 Place the cap on the rotor and tighten to 12N m Socket adapter with the torque wrench H ket amp Using SEAL TUBE in P50VT2 P65VT3 P9ONT ETETE EE P65NT P65NT2 Rotor 1 Fix the projection of the handle to the hole of the cap 2 Tighten the cap securely with the handle 3 Remove the cap handle Don t operate the rotor with the cap handle Using RE SEAL TUBE CONE TOP TUBE Cap 1 Place the cap packing and the cap on the in tube EA _ handle in the rotor eT 2 Tighten to 8N m with the cap handle and the Rot Cap torque wrench on Cap The cap pushs down the tube is sealed So 0 packing tighten the cap securely to prevent leakage Det h 1N m 10 2Kgf cm Torque N CAUTION If the number of tubes to be set is mener small do not install the space cap or Cap 7 ei handle the cap in the tube cavity into which no tube is inserted but stay the Cap cavities uninstalled for operation Cap ert packing Tube Rotor Operation 1 Gently and securely set rotor onto the drive shaft 2 For operation refer to the instruction manual of the preparative ultracentrifuge Longer acceleration and deceleration will result in better separation If you start with homogeneous solution slow deceleration only For more detail refer to the instructions of the centrifuge 5 Removal of sample 1
69. tical adapter or disk is unclean or damaged The ROTOR alarm lights Wipe it with a soft cloth Replace the adapter of disk if damaged on the centrifuge If the optical adapter needs replacement call the Sales Agent To replace the optical disk see 4 4 Inspection of Rotors replacing the optical disk The IMBALANCE alarm Is the sample well balanced lights on the centrifuge Isn t the sample taking The SPEED alarm lights Isn t the speed set higher than the maximum permissible value on the centrifuge If another alarm lights on the centrifuge refer to the operating instructions of the centrifuge If the rotor needs to be repaired it must be sent to the factory So call a Hitachi Koki authorized sales service representative immediately Before shipping the rotor be sure to sterilize and wash it 5 2 Tubes The tube can be deformed or damaged by varying causes Check the following items in the order described Additionally see 2 6 Inspection and 2 7 Life 1 Isn t the amount of the sample too small See 2 8 Howto use Tube and Bottle 2 9 How to balance 2 Isn t the speed higher than the maximum permissible value See 3 1 Allowable Rotor Speeds and the rotor instruction manual 3 Are the cap and space cap etc combined properly Are the parts of the cap combined properly See appendix 1 appendix 2 appendix
70. uckets from the rotor PE65ST Unscrew the pin with the driver SAI rotors except P65ST Remove the bucket by hand 3 Remove the cap from the bucket with the driver or the cap spanner 4 Take tubes out with pincettes 5 Recover supernatants or pellets ZN CAUTION e Each time the rotor with optical adapter is used be sure to enter the results in the rotor log book Failure to do so will disable management of the life of rotor No entry is needed for the rotor with RLM adapter e Perform maintenance and inspection of the rotor each time it is used If there is any abnormality immediately stop using the rotor and contact a Hitachi Koki authorized sales service representative Refer to 4 2 Maintenance of Rotors and 4 4 Inspection of Rotors 42 P32ST rotor top loading type buckets Preparation 2 Prepare the tubes See 2 8 How to use Tube and Bottle 1 Check the rotor Is a bar damaged Are the threads worn e Is there a packing 3 Prepare accessories Is there foreign matter in the tube cavities Bucket stand Tweezers Is the end of the groove for the bucket bar deformed Does any foreign matter adhere on the end of the groove for the bucket bar 1 Remove the cap from the bucket 3 Tighten the cap of the bucket Stand the bucket in the bucket stand Tighten the numbered cap to the same numbered bucket by hand to match each mark of the bucket and the cap
71. urn the cap to introduce the stopper into the slit of the stem on the cap If you tighten the nut at wrong position the stopper may be damaged Examples of correct Nut Step 9 Crown position and wrong position on setting TU Correct Tube Wrong the stopper are shown in the below figures Stopper Stopper Cap E Slit Ge Cap I N SG SN E SY Handle A Correct position Wrong position 3 Tighten the nut with a torque wrench or a box wrench If using the tube vise with the stopper tighten the nut with a torque wrench with a clutch as holding down the stopper by your finger e Torque wrench e Torque wrench with a clutch e Torque wrench EEE A P EI Tighten to function the Tighten to a specified tightening torque clutch of the torque wrench e Box wrench Tightening tools are specified for Tighten the nut different caps see appendix 4 securely Table 2 4 Specified tightening torque Part No of cap Name of cap Volume of applied tube Tightening Torque 339673A E T12 Cap 40mL tube 305231A E3 ALCap 40mL tube 6N m S308090A E4 ALCap 40mL tube 322690A F AL Cap 94 mL tube 325968A F2 ALCap 94 mL tube 463577A C Tl Cap 12 mLtube 474070A B2 TI Cap 6 5 mL tube 10N m 481649A C2 TlCap 12 mL tube 474272A E TI Cap 40 mL tube 12Nm 1N m 10
72. usable Tubes and bottles Caps Space caps PA PPPEPET SS Spacers Adapters Screw Caps of Washing conditions bottles O ring Washing solution pH5 or less Washing solution between pH5 and pH9 Washing solution pH9 or above Hot water 50 C or less Ultrasonic washing Neutral detergent pH7 Drying condition Drying in the air N CAUTION In all cases use neutral detergent between pH5 and pH9 2 4 Sterilization Refer to Table 2 2 for the sterilization of tubes and caps eHow to autoclave tubes and bottles 1 Wash tubes and bottle well 2 Stand it in the heat resistance rack Get off screw caps and inner caps of bottles to prevent deforming 3 After autoclaving take out tubes and bottles after the temperature in the tank of autoclave lower to room temperature Table 2 2 Sterilization conditions O Satisfactory x Unsatisfactory Caps for bottles Tubes Bottles Sterilizing condition Metal Plastic Caps PET caps O rings O Oa ZO gt VU O U m U VU GO n 115 C 0 7kg cm for 30 min Autoclaving 121 1 0kg cm for 20 min 126 C 1 4kg cm for 15 min Boiling 15 30 min Ultraviolet rays 200 300 min Ethylene oxide Gas Formaldehyde Ethanol 70 OIK ORK OIK olo OIK ORK OIK olo GOIO OIK TK x x x x O ol olo x x I x x x Gu O GO OJ X O X OJ O O O0 X O OJ O X GA O Gu GO GO D O O

Download Pdf Manuals

Related Search

Related Contents

Southbend EZ-3/EZ-5 User's Manual  4863 Trousse principale d`outils de réglage de moteur  DB Drive Car Amplifier 3 CHANNEL AMPLIFIER User's Manual  CFW11 - Adendo Grau de Proteção IP54    Masterpact Modbus User manual 2011  Shure KSM42 User Guide  Tripp Lite DisplayPort to HD Cable Adapter (M/M), 6-ft.  Fujitsu PRIMERGY TX150 S7  Advanced Bash-scripting Guide  

Copyright © All rights reserved. Failed to retrieve file