User Manual MyoVIEW
Contents
1. www dmt asia com sales dmt asia com support dmt asia com DMT Asia China office Rm 28C No 8 Dong Fang Road Lu Jia Zui Financial District Shanghai 200120 P R China El e 0 21 5425 1330 Fax 86 0 21 5877 0063 www dmt asia com sales dmt asia com supportedmt asia com DMT USA Inc 525 Avis Drive Suite 10 Ann Arbor MI 48108 USA Tel 1 770 612 8014 1 678 302 7013 www dmt usa com sales dmt usa com SupportQdmt usa com2. tinue for 10 seconds as listed and then check if the pressure at P1 has reached 40mmHg if not the task will continue until the 40mmHg has been reached Figure 2 26 Task manager 3 Enter all the tasks needed in the sequencer and click OK The sequencer tasks are listed in the trace as marks marked in figure 2 28 below Edit Task Double click on task and the Task Manager will show the task to be edited Alternative mark the task and select edit task in the drop down menu of the sequencer see figure 2 25 Description Step 2 40mmHg V Show description on trace when task starts Duration 10 s Wait Delete a Task Mark the task and select delete task in the drop down menu of the se e mm x quencer B Load Sequence Select Load Sequence in the drop down menu of the sequencer dE Save Sequence Select Save Sequence the drop down menu of the sequencer Clear Sequence sequence can be deleted Select Delete Sequence in the drop down Figure 2 27 Task manager menu of the sequencer wait box Loop Sequence A sequence can be repeated again and again using the Loop Sequence function Select Loop Sequence in the drop down menu of the sequencer LIG M MyoVIEW for DMT Pressure Myograph Systems Zone 1 1 Diameter MyoVIEW Data View Auto Threshold Camera DMx 41AU02 F1 F4 F7 F10 Invert image Play video from file F2 FS F8 F11 Settings Ignore Inner Diameter F3 F6 F9 F12 Analy
3. Open experiment Open a saved MyoVIEW experiment Save experiment Save the MyoVIEW experiment as a Myo file Close experiment Close the MyoVIEW experiment Exit Exit the MyoVIEW programme 3 2 MyoVIEW Quick Access Toolbar The MyoVIEW quick access tool bar is in the top left corner see figure 3 2 below f As m Mb Figure 3 2 MyoVIEW Quick Acces Toolbar Customize Quick Access toolbar Customize the Quick Access Toolbar see figure 3 3 below Show Below the Ribbon v Minimize the Ribbon Figure 3 3 Customize Quick Acces Toolbar 20 MyoVIEW USER MANUAL 3 3 New trace By clicking the New Trace Icon at the MyoVIEW Quick Acces Toolbar a new trace will be shown in MyoVIEW The new trace will not be dedicated to show any specific parameter before this is selected Before opening the first trace 1 Select Record Video in the Video Recording submenu of the Data Menu if video of the experiment should be recorded and Saved 2 Start Video Capture in the Camera Control Window 3 Mark one or two zones on the vessel to be recorded in the Image Control Window To select and show a trace of a specific parameter right click on the trace and the following will be shown see figure 3 4 below Myoview Data View oVIEW for DMT Pressure Systems Zone 1 None h Syst Auto Threshold Invert image Ignore Inner Diameter Analysis Source Camera DMx 41AU02 Play video from file
4. Play video from file F2 FS F8 F11 Settings Ignore Inner Diameter F3 F6 F9 F12 Camera DMx 41AU02 Brichtness Gamma Gain 0 Calibration Magnification 10 0 Force mN Current 24 7 Zero Force Camera VW ja Sequencer not started Fi F2 F3 FS F6 F7 F8 F9 F10 F11 F12 Figure 2 4 Camera Control window Calibrate Camera 10 MyoVIEW USER MANUAL 4 The micrometer ruler will now be displayed on the screen Select the Magnification marked in figure 2 5 below correspond ing to the objective used on the microscope 5 Draw a box covering the 1000 ruler exactly as shown in figure 2 5 red box below using the mouse holding the left mouse button down The appearing red box with the tick marks can be rotated 90 degrees by clicking either the Width or Height on the right hand side marked in figure 2 5 below to be able to get the red box to fit the ruler for every 200um see figure 2 6 below ret 29067 a 0 Figure 2 5 Calibrate Camera micrometer ruler Figure 2 6 Calibrate Camera micrometer ruler rotated 90 degrees 6 Fine adjust the red box tick marks by clicking on the plus and minus slider marked in figure 2 7 until it fits exactly Figure 2 7 Plus and minus slider f Click on Calibrate and fill in the number of um the red box covers into the box Enter measured microns Press the ENTER key
5. Select parameter Trace data of given parameter from zone 1 or zone 2 is selected by clicking on the small box 1 or 2 on the left hand side of the trace marked below Figure 2 14 Zone 1 and Zone 2 Select the zone to be shown 14 MyoVIEW USER MANUAL The zones can be moved enlarged or minimized as needed by dragging the box As soon as the box is placed the wall tracking algorithm in MyoVIEW will start trying to track the inner and outer wall of the vessel The red line will show the tracking of the outer wall and the green lines will show the tracking of the inner wall To the far right in the Image Analysis Control window the outer red lines and inner diameter green lines are shown for the two zones see figure 2 15 below If the tracking of the inner and outer wall is not correct then the algorithm can be fine tuned by highlighting the zone box and thereafter change the Course Edge Detection using the slider or the minus and plus sign in the Image Analysis Control window as marked in figure 2 15 below until the tracking of the inner and outer wall is perfect see figure 2 16 Image Analysis Figure 2 15 Use the Course Edge Detection Slider to adjust the tracking Image Analysis Camera v Recording 0 Fine Edge Detection Inner Diameter Outer Di VU 2 Course Edge Detection ameter Figure 2 16 Here the slider Course Edge Detection has been changed for zone 2 to get a b
6. to save the calibration of the camera in this case it is 1000um see figure 2 8 _ 2 gt gt ee oo Ld E 4 J Ey 17 Ihi Magnification 10 0 um pixels Figure 2 8 Enter measured microns CHAPTER2 11 8 In the box Measurement um marked in figure 2 9 below the camera will now Show how many um the red box covers This value should in this case be 1000um By resizing the red box the calibration of the camera can be controlled by reading the number in the Measurement um when the red box is resized Figure 2 9 Measurement um box The camera calibration will be saved as an action in the Action Sheet to document that the camera calibration has been per formed In the example shown below we have calibrated the camera three times just for illustration or DMT Pressure Myograph Systems Action Sheet Figure 2 10 Action sheet illustration of saved calibration of the camera 12 MyoVIEW USER MANUAL 2 3 Image Analysis Control Window The Image Analysis Control window is showing the video of the mounted vessel This video image is used to measure and cal culate the parameters recorded in MyoVIEW Figure 2 11 Image analysis control window 2 3 1 Zones Before MyoVIEW can calculate the dimensions of a mounted vessel the user has to define a region wherein MyoVIEW will track the vessel wall and calcul
7. use cgs standards such as dynes VESSEL FLOW RECORDING TASK Intermediate units engem I Solution presets constants Density 9 937e 1 Viscosity 6 904e 4 g cm x s UNIT CONVERSIONS Input Units to Intermediate Units Conversions Volume Flow n Q cm Intermediate Units to Output Units Conversions Vascular Resistance dyne s cm 5 Vascular Resistance 0 100 MPa s m 3 Vascular Resistance is commonly reported in either dyne s cm gt or MPa s m or both FOR FURTHER READING Kristopher S Cunningham and Avrum I Gotlieb The role of shear stress in the pathogenesis of atherosclerosis Laboratory Inves tigation 2005 85 9 23 Please note that the units given for Re in this reference are wrong 36 MyoVIEW USER MANUAL APPENDIX 2 MYOVIEW UPGRADES OR CHANGE LICENSE MyoVIEW upgrades is free within the same version number e g if version 3 has been purchased all upgrades up to version 3 9 9 will be free of charge When DMT release new version number complete installation of new the MyoVIEW version will be per formed keeping the old version A question mark icon is shown in MyoVIEW in the right corner see figure A2 1 Clicking this icon will open the License Manager of MyoVIEW as seen in figure A2 1 below Here the license version installed can bee seen Furthermore the MyoVIEW license can be either upgraded or changed Upgrad
8. DANISH MYO TECHNOL Ost MYOVIEW SER MANUAL D MyoVIEW Data View Show Analysis Window Show Data Sheet Time so Export Type Mean Value Zone border color Reset window layout Show Action Sheet top live trace Export to Data Sheet Outer edge color V Show Status Bar Select all traces QU expand vie Inner edge color Application layout Data Analysis Live Traces Trace selection Zone colors DMT Microscope X Camera ax Pressure Interface X Flow Meter ax IR Light DMx 41AU02 Capture Heater of OMT Flow Meter 211 Current Target Flow i 15 g 254 370 en re Pressure os Current Target 799 P2 79 9 80 0 Gradient 10 0 Calibration Flow os Magnification 10 0 Force mN Current 0 2 Zero Force Image Analysis ax Camera w Course Edge Detection Fine Edge Detection Inner Diameter Outer Diameter Sequencer Z d 4 J i pi i 1 ii 1 L i 1 j ii ii F1 li i L 1 1 11 t 7 A User Manual Version 1 0 TRADEMARKS Danish Myo Technology A S reserves the right to alter specifications as required This document was as far as possible accurate at the time of printing Changes may have been made to the software and hardware it describes since then New information may be supplied separately No part of this document may be reproduced by any means without the prior written permission of D
9. ER 5 DATA ANALYSIS AND EXPORT MyoVIEW will record and save data for all parameters during an experiment Open a given experiment and data file in MyoVIEW The open file will show you the same layout as shown when the file was saved Below is an example Da X MyoVIEW for DMT Pressure Myograph Systems Zone 1 Inner Diameter 2 myoview Data FT Auto Threshold Camera DMx 41AU02 Fi F4 F7 F10 Invert image Play video from file F2 FS F8 F11 Settings FT Ignore Inner Diameter F6 F9 F12 Analysis Source Markers 2 1 1 Diameter x Action Sheet Z E g E 8 E k nr i 8 8 v o E 5 0 9 18 27 36 45 1 21 1 30 Sequencer ax Sequencer VW Stare Pause Task Time p P2 Temp Wait Prompt M M Y NAE EM a V step1 20mmHg 10 20 20 37 No No V Step 2 40mmHg 10 40 40 37 No No Step 3 60mmHg 10 60 60 37 No No V Step 4 80mmHg 15 80 80 37 No No ax 4 Course Edge Detection 1 T 2 148 microns Fine Edge Detection ho 9 Inner Diameter f 0 gt Outer Diameter vi rd gt T nn Sequencer ended F1 F2 F3 F4 FS F6 F7 FS F9 F10 Fil F12 Figure 5 1 Example of an open file To look at other parameters of the experiment Open a new trace and right click on the trace to select the paramete
10. NESS AND GAMMA VALUES CANNOT BE CHANGED ON A RECORDED VIDEO FILE 3 4 1 3 Markers submenu e F1 F12 Linked to predefined Marker Strings e Settings Enter Marker text for the given Hotkeys In MyoVIEW markers can be added to the traces in two ways To enter a free text at a given position in a trace double click on the trace and this will open a text box as shown on next page 24 MyoVIEW USER MANUAL Auto Threshold Invert image Ignore Inner Diameter Analysis Figure 3 11 Analysis submenu Camera DMx 41AU02 v Play video from file Source Figure 3 12 Source submenu F1 F4 F7 F10 F2 FS F8 F11 Settings F3 F6 F9 F12 Markers Figure 3 13 Markers submenu Enter any given text of choice and press Save message or press the ENTER key MyoVIEW for DMT Pressure Zone 1 Inner Diameter Figure 3 14 Markers enter free text The entered text will be shown at the given position in the trace as marked in figure 3 15 below MyoVIEW for DMT Pressure Myograph Systems Zone 1 Inner Diameter Figure 3 15 The entered text shown at given position CHAPTER3 25 The other possibility in MyoVIEW is to predefine Marker text and link them to Hotkeys This functionality is very useful for Marker text used again and again In the Markers submenu press settings and the dialog box below will be shown Marker strings Hotkey otkey F Hotkey F1 Hot
11. Show Action Sheet Stop Live Trace Export to Data Sheet Outer edge color F Select all traces QJ amp Expand View Inner edge color Data i Live Traces Trace selection Zone colors Reset window layout 1 E 3 V Show Status Bar Application layout Figure 1 8 Reset window layout 8 MyoVIEW USER MANUAL CHAPTER 2 CONTROL WINDOWS The MyoVIEW control windows control the Camera the Image Analysis the Pressure Interface the Sequencer FlowMeter op tional and DMT Inverted Microscope optional 2 1 Camera Control Window The capture of video images from the video camera is activated by pressing CAPTURE marked in figure 2 1 A below and stopped again by pressing STOP marked in figure 2 1 B below in the Camera control window The Brightness Gamma and Gain of the camera can be adjusted using the appropriate sliders see figure 2 1 B below to obtain a good image of the mounted vessel Camera n x Camera n x psi oru plikt omxarauoz capture ser Bnr mero Briahtness Gamma Gain Calibration Calibration Magnification 10 0 Magnification 10 0 Calibrate Figure 2 1 Camera control window Figure 2 1 B Camera control window Capture Stop The best image to obtain good tracking of the vessel walls is images in the grayscale area and not very bright images showing a black and white imag
12. anish Myo Technology A S Copyright O Danish Myo Technology A S DANISH MYO TECHNOL GG 2 MyoVIEW USER MANUAL CONTENTS raden 2 Chapter 1 Start UD a a NE e e a 2 TIMEN Start ub SVT COMDUTET a EE 4 EE m 5 1 2 1 Reset Of EE NE NN a 8 Chapter 2 GN WINOOWS e 9 LEG Se COM TOL WOO ae e meme 9 NENNE 10 2 3 SSE Se OE Doro EE EN NE Tm 15 EG ee EE 13 STE NNN VS CIN ON mek a a a ih 16 LEG FO Ne 2 Be aes S e ES ER EN OE VE ee NER 16 2 54 Snapshot image of the Mounted VOSS Lue Reti GR SAN Cdi sun abnorme 16 2 4 Pressure Interface Control WiNQOW estan rina EFE nM Econ 16 Vicus ME e Rie MNT m u 17 edu We SSC Oe GS e a E a EU E 1f vaio M P 17 2 5 96dq uencer AL L ON EU 17 2 6 Flow meter Control window optional n 19 2 7 DMT Inverted Microscope Control Window Optional ssssccccccssesesececccecesseeseeccccesseesenececessnerenececeseeesenececesssarseececessserenesees 19 CHAPTER THE MyoVIEW men
13. ate all the parameters In MyoVIEW a zone is placed by left clicking and dragging a box across the video image of the vessel in the Image Analysis Control Window marked in figure 2 12 below MyoVIEW can handle up to two zones In each zone all the parameters are tracked measured and calculated individually Each zone will have a number 1 or 2 In figure 2 12 below two zones have been placed and in the left side of the trace window a 1 and 2 can be seen representing zone 1 and zone 2 respectively marked in figure 2 12 below To see the trace of a parameter from zone 1 click on the 1 and then select the parameter to be shown by right clicking on the trace see figure 2 13 When the measuring zone is placed on the video image of the vessel in the Image Analysis Control Window a Trace recording will be opened automatically see figure 2 12 below without any selected parameter if not opened manually before MyoVIEW Data View Auto Threshold Camera DMx 41AU02 lt FL F4 F7 F10 Invert image Play video from file F2 F5 F8 F11 Settings F3 F6 F9 F12 Source Markers Figure 2 12 Image Analysis Control Window Trace recording CHAPTER2 13 Right click on the trace to select the parameter as shown below MyoVIEW for DMT Pressure Myograph Systems None Zone1 Zone2 None Left Wall Thickness Right Wall Thickness Average Wall Thickness Figure 2 13
14. data sheet from the selection shown in the given trace MyoVIEW for DMT Pressure Myograph Systems Zone 1 Inner Diameter Figure 3 30 Selection of the given trace CHAPTER3 29 See Data Sheet marked in figure 3 31 below MyoVIEW a MyoVIEW for DMT Pressure Myograph Systems Data Sheet Data View i Show Analysis Window Show Data Sheet Time Secs 85 Show Action Sheet Start Live Trace Select all traces QuU Live Traces Zone border color Outer edge color Export Type Mean Value Export to Data Sheet Expand View Trace selection Reset window layout Show Status Bar Application layout Inner edge color Data Analysis Zone colors Zone 1 Inner Diameter Data Sheet x I Mean Value Duration Sec Inner Diameter 1 Outer Diameter 1 56 851 113 596 Average Wall Thickness 1 113 000 Figure 3 31 Export of data sheet of one given trace 3 4 3 3 Live Traces submenu Time secs Number of seconds shown on the recording traces If 100 seconds is typed in then the last 100 seconds of the running traces are shown Start Live Trace Stop Live Trace Stop Live Trace will stop the refreshing of the trace and let the current trace freeze making it possible to look carefully at a part of the curve while the data still being recorded Start Live trace will always show the front of the trace where new data is being recorded into the trace Scrolling Bar The scrolling bar is
15. e Keep the light and brightness to the lower side and keep the image in the grayscale instead of a very bright image resulting in completely white background The latter will result in poor quality tracking of the wall see examples in figure 2 2 A B below Image Analysis Camera VW 1 4 37 Recording Figure 2 2 Good image for tracking Figure 2 2 B Too bright image and the of the vessel wall tracking will not be good CHAPTER2 9 2 2 Calibration of the camera It is very important that the camera is calibrated to obtain the pixel um relationship for the objective used on the given camera Once the camera is calibrated with the given objective the calibration data will be saved in MyoVIEW A new camera calibration is only performed if the objective or camera is changed or never have been used before 1 Press CAPTURE in the Camera Control Window to start the Video Capture 2 Place the supplied micrometer ruler on the microscope and adjust the microscope to get the ruler in focus DMT Inverted Mi croscope users place the micrometer ruler on the supplied micrometerpositioner ruler adaptor as shown in the picture below Figure 2 3 Placing the micrometerpositioner ruler on DMT Inverted Microscope 3 To calibrate the camera press the Calibrate icon in the Camera Control window marked in figure 2 4 below SO MyoVIEW Data 1 Auto Threshold Camera DMx 41AU02 F1 F4 F7 F10 V Invert image
16. e in the center of the viewing field on a given trace or click on the UP and DOWN arrow icons to move the Y axis up or down b CHAPTER 23 3 4 Menus In the MyoVIEW program there are three main menus in the toolbar MyoVIEW Data and View marked in figure 3 10 below MyoVIEW Data View I Auto Threshold Camera DMx 41AU02 v Fl F4 E7 EIQ Invert image Ignore Inner dd Play video from file F3 F6 F9 F12 F2 FS F8 F11 Settings Analysis Source Markers Figure 3 10 MyoVIEW Menus 3 4 1 MyoVIEW Menu In the MyoVIEW Menu there are three submenus Analysis Menu Source Menu and Markers Menu 3 4 1 1 Analysis submenu e Auto Threshold Selecting Auto Threshold will disable the manual setting of the track ing parameters in the tracking algorithm and use the default parameters The Auto Threshold will only be optimal for very clean vessels e Invert Image Invert the pixel values on the video input making white black and visa versa Ignore Inner Diameter The inner diameter will not be calculated This should normal ly be selected for very thick vessels as e g aorta where tracking of the inner diameter is very difficult 3 4 1 2 Source submenu Camera Selection of camera for now DMT only support one type of camera e Play video from file A recorded video file from e g a former pressure experiment can be loaded shown and also reanalyzed using MyoVIEW NOTE THE GAIN BRIGHT
17. e license This is used for upgrading MyoVIEW with modules in the the basic version of MyoVIEW e g as the FlowMeter Module A new license code will follow and be delivered with an investment in a FlowMeter as a new module for the Pressure Myograph System The MyoVIEW license will have to be upgraded with this new license MyoVIEW Version 3 0 2 0 Copyright C 2013 Danish Myo Technology Licensed to DMT Rene Hummel Upgrade License Change License MyoVIEW is protected by copyright law and international treaties Unauthorized reproduction or distribution of this program or parts of it may result in severe civil and criminal penalties and will be prosecuted to the extent possible under the law Figure A2 1 Upgrade or change MyoVIEW licens Change license Used to shift between MyoVIEW for the Pressure Myograph System and the Culture Myograph System Figure A2 2 Change license APPENDIX2 37 Customer Name DMT Rene Hummel License 49EA19F0046E E9433815CE7A 8D6C9C9F418B 17DBE7110996 Figure A2 3 Fill in license and click change license 38 MyoVIEW USER MANUAL NOTES NOTES 39 DMT A S Skejby Science Center Skejbyparken 152 DK 8200 Aarhus N Denmark Tel 45 87 41 11 OO Fax 45 87 41 11 01 www dmt dk sales dmt dk support dmt dk DMT Asia Ltd Rm 2402B Great Eagle Centre 23 Harbour Road Wanchai Hong Kong S A R P R China Tel 852 6621 8337 Fax 852 3020 7554
18. e real time parameters and how they are relevant to vessel physiology and vascular function WALL THICKNESS D DY 2 D is the outer or external diameter of the vessel and D is the luminal or inner diameter Wall thickness can be used as an index of vascular hypertrophy the thicker the wall the greater the degree of hypertrophy CROSS SECTIONAL AREA 1 4 D D D is the outer or external diameter of the vessel and Di is the luminal or inner diameter Similar to wall thickness cross sectional area can be used as an indication of hypertrophy or vascular wall remodeling MEDIA LUMEN RATIO D D D D is the outer or external diameter of the vessel and D is the luminal or inner diameter The media lumen ratio is indicative of morphological changes and can be used along with wall thickness and cross sectional area to determine the particular form of hypertrophy MEAN PRESSURE P TPA ORR EP P or P is the input or inlet pressure and Por P is the output or outlet pressure The mean pressure is the average of the inlet and outlet pressures It is necessary to monitor when flow is induced by a pressure gradient or differential between P and P Mean pressure should be constant when measuring flow mediated dilation to verify that increases in vessel diameter are flow induced and not pressure induced VASCULAR RESISTANCE Par Q is the pressure difference and Q is the flow volume The vascular resistance is the
19. etter tracking of the vessel walls In cases where connecting or fat tissue can not be removed perfectly from the vessel the tracking of the walls could be problem for the tracking algorithm In these cases the Fine Edge Detection sliders in the Image Analysis Control window is used See figure 2 17 below There is one slider for each of the four tracking lines By sliding the Fine Edge Dection bar offset values are added or subtracted to the tracking algorithm and thereby the user will be able manually to place the tracking line to the position where the user can see the wall even though there is lot of connecting tissue attached to the vessel Fine Edge Detection Inner Diameter jr jon E Outer Diameter Figure 2 17 Fine Edge Dection CHAPTER2 15 2 3 2 Tracking problems and solutions 1 If there is problem with the tracking even after changing the Course Edge Detection parameters then try to change the camera gain and brightness combined with the Course Edge Detection parameters Adjusting the light intensity on the mi croscope Is also an option to get better tracking of the vessel walls 2 Very large thick vessels are too thick to light through making it impossible to track the inner diameter An example would be rat aorta In this case select Ignore Inner Diameter in the MyoVIEW Menu MyoVIEW will then only track the outer diameter 3 The vessel is not clean The vessel has some connec
20. force opposing the movement of solution through a vessel It is inversely proportional to the vessel radius to the 4 power A greater vascular resistance will require a greater degree of vasodilation in order to maintain constant pressure within the vessel SHEAR STRESS 8 n V D The constant n or eta sometimes represented as or mu is the dynamic viscosity V is the flow velocity and Di is the inner diameter of the vessel Shear stress is a characterization of the frictional drag exerted on arterial walls during flow Many physi ological functions are influenced and promoted by this biomechanical force Normal levels of shear stress serve to maintain wall physiology High shear stress triggers vascular dilation in order to regulate the mechanical forces exerted on arterial walls APPENDIX 1 35 APPENDIX The following example is given for water at 37 C to help with the conversion of input to intermediate to output units Input units will be determined by either the hardware attached or the task set up in the software For example all units of length will input as micrometers if the dimensioning task is set up this way in the task manager The output units were taken from survey of the primary literature The selection of intermediate units is personal preference In this example cgs units have been chosen i e centimeters grams and seconds as the intermediate units because most of the output values taken from the literature
21. ged between 1mmHg sec and 10mmHg sec Flow On Off Inside the Pressure Interface a valve will normally be closed blocking the flow coming from the P2 side of the chamber and create a no flow situation Simulate Blind sac experiment The flow is turned on by a click on the On icon and off by a click on the Off icon In the no flow state only the Pressure entered as the P1 value will be applied to the mounted vessel NOTE WHEN MYOVIEW OPENS THE FLOW IS ALWAYS OFF AS A DEFAULT 2 4 3 Force Force mN Zero The force transducer measuring the longitudinal force generated by the mount ERR EE ed vessel is calibrated as described in the Pressure Myograph Systems User Manual In MyoVIEW the zero is used to zero the force of the force transducer Besides using the force transducer for measuring the longitudinal force generated during an experiment Figure 2 23 Force the force transducer can also be used for normalizing the vessel during mounting of the vessel To make sure that all vessels are strectched equally each time the force can be used to put the same stretch force to the vessel before starting an experiment 2 5 Sequencer Control Window The Sequencer in MyoVIEW is a feature to program the Pressure Interface to define pressures after a defined time period A se quence will normally contain several steps programs tasks The sequencer is found at the bottom of the MyoVIEW Layout see figure 2 24 below Figure 2 24 Sequencer con
22. key F2 Marker text Hotkey F3 Hotkey F4 Hotkey F5 Hotkey F6 Hotkey F7 um Hotkey F8 Hotkey F9 Hotkey F10 42 Hotkey F11 Hotkey F12 Figure 3 16 Marker strings Here the Hotkey Function key is selected and the appropriate text linked to a Hotkey is entered Below the text Drug 1 has been connected to Hotkey F1 Every time the F1 Hotkey is pressed during an experiment the text Drug 1 will be added to the trace as a marker at the given time There is in total 12 Hotkeys F1 F12 that can be predefined with a user selected Marker text of choice Figure 3 17 Hotkey 3 4 2 Data Menu In the Data Menu there are three submenus Export Delete and Video Recording D MyoVIEW Data View Export to Excel Clear Data Sheet Synchronise data Record Video Export to CSV file Clear Action Sheet Video Quality Medium Y Copy to Clipboard Export Video Recording Figure 3 18 Data Menu 26 MyoVIEW USER MANUAL 3 4 2 1 Export submenu Export to Excel This submenu will export all the data selected in the Data Sheet into Excel By clicking Export to Excel Excel only if installed on the computer opens up and all data from the MyoVIEW data sheet will be exported into an Excel sheet Export to CSV file This submenu will export data in the Data Sheet into a comma separated file By clicking the Export to CSV file icon the following will be shown if dif ferent export types Mean Maximum and Minimu
23. m have been used to generate the data in the Data Sheet Select the data sets to export and save the CSV file Copy to clip board This function will copy the data in the data sheet to the clip board and can then be pasted into an appropriate program as e g Word Excel and PowerPoint 3 4 2 2 Delete submenu Clear Data Sheet This will delete all the data in the Data Sheet NOTE THE DATA FROM THE DATA SHEET CANNOT BE RECOVERED IF CLEAR DATA SHEET IS USED Clear Action Sheet This will delete all the data in the Action Sheet NOTE THE DATA FROM THE ACTION SHEET CANNOT BE RECOVERED IF CLEAR ACTION SHEET IS USED 3 4 2 3 Data Collection submenu Synchronize data This make sure that all recorded data is synchronized in respect to time MyoVIEW will check for hardware connected Pressure Interface Camera Flow meter and dependent on the hardware identified the maximum data collection rate possible will be set and all parameters will be recorded as synchronized data sets in respect to time The actual set sampling rate will be written in the action sheet as shown in figure 3 23 below The data will be recorded as synchronized data as soon as the Synchronise data box is checked When the Synchronise data box is unchecked all incoming data will be collected at maximum speed for each parameter and a line in the Action Sheet with the text Data Samples not synchronised will be added with a time stamp Export to Excel Export t
24. ner Diameter Figure 3 6 Trace Layout 1 Tab layout MyoVIEW for DMT Pressure Myograph Systems Zone 1 Outer Diameter Figure 3 7 Trace Layout 2 Column Layout 22 MyoVIEW USER MANUAL D A i z MyoVIEW for DMT Pressure Myograph Systems Zone 1 Outer Diameter MyoVIEW Data Auto Threshold Camera DMx 41AU02 1 F4 F7 F10 T Invert image Play video from file F2 FS F8 11 Settings M Ignore Inner Diameter F3 F6 F9 F12 Analysis JU Source J Markers Zone 1 Inner Diameter x 2 E Figure 3 8 Trace Layout Rows Layout 3 3 1 Setting of the Y axis Click on the Y axis icon at the left hand side It will open a Y axis setting menu as shown below Trace be Minimum 0 Maximum 100 V Auto range Unit type pm V Show unit type Figure 3 9 Y axis setting menu Trace Type Select the parameter to be shown Vertical limits Enter the minimum and maximum Y value Auto range Will at all time scale the Y value data shown in the trace automatically Unit type Measure unit This cannot be changed but shows the unit of the given parameter Show unit type Will show the unit of the given parameter on the y axis of the trace e g inner diameter will show um Label count The distance between tick marks on the y and x axis can be entered Move Y axis up or down Scrolling the mouse wheel moves the Y axis up or down This is a fast and easy way to get the trac
25. o CSV file Copy to Clipboard Export Figure 3 19 Export submenu Export data to CSV file Figure 3 20 Export data to CVS file Clear Data Sheet Clear Action Sheet Delete Figure 3 21 Delete submenu Synchronise data Data Collection Figure 3 22 Data Collection submenu Inner Diameter Action Sheet x Action Description Data collection stopped Synchronised data collection enabled Sample frequency set to 10 hz Figure 3 23 The actual set sampling rate written in the action sheet CHAPTER 27 3 4 2 4 Video Recording submenu Record Video Selected if the video signal from the USB camera should be saved to gether with all the trace recordings of an experiment If this is not selected you will not be able to start video recording during your experiment without first stopping the Video Capture save the data and start new file VERY IMPORTANT IF THE VIDEO FILE OF THE EXPERIMENT SHOULD BE SAVED IT IS EXTREMELY IMPORTANT TO SELECT RECORD VIDEO BEFORE STARTING THE VIDEO CAPTURE IN THE CAMERA CONTROL WINDOW Video quality The video of the experiment can be recorded in three qualities Low Medium and High The size of the recorded video file will increase significantly from a low to a high quality file An example is given here A 10 minutes video file size Low quality 10MB e Medium quality 5OMB High quality 150MB Free disk space availabilit
26. p 39 CONTENTS 3 CHAPTER 1 START UP The DMT Pressure Myograph Systems is primarily operated via the MyoVIEW software The function of the software is to control the heat of the chamber the pressure and the trace recording of following parameters e Left Wall Thickness e Right Wall Thickness Average Wall Thickness Outer Diameter nner Diameter Vessel Area e Lumen Area e Cross Sectional Area e Media Lumen Ratio e Shear Stress optional nlet Pressure e Outlet Pressure Mean Pressure Flow optional e Vascular resistance optional Temperature Force longitudinal pH optional 1 1 MyoVIEW Start up on your computer Go to Start gt All Programs gt DMT gt MyoVIEW NOTE RIGHT CLICK ON MYOVIEW AND SELECT SEND TO TO CREATE A MYOVIEW SHORTCUT ON YOUR DESKTOP MyoVIEW will start up with the layout from last time MyoVIEW was used The following MyoVIEW screen will now be visible on the screen see figure 1 1 or 1 2 MyoVIEW for DMT Pressure Myograph Systems Calibration Magnification 10 0 Calibrate Figure 1 1 Example of MyoVIEW start screen with DMT microscope attached 4 MyoVIEW USER MANUAL MyoVIEW for DMT Pressure Myograph Systems Calibration Magnification 10 0 Calibrate DMT MER Te Figure 1 2 Example of M
27. p and down see figure 1 6 and 1 7 The layout in MyoVIEW is extremely flexible and user friendly MyoVIEW saves the given layout shown on the screen when the program is closed 6 MyoVIEW USER MANUAL D ne vider r2 f f mE E OM MN MN MN MN ME MN EN l Image Analy Camera V 1 1 Course Edge Detection I I I Fine Edge Detects I Taner Diameter I Outer Diameter I I I 1 10 1147 I I ETER 4 Sequencer not started F1 F2 F3 F4 FY FG F FO FIO F11 F12 Start 09 46 44 Figure 1 6 Image Analysis window at the buttom of the MyoVIEW screen F Auto Threshold Camera DMx 41AU02 Vi EKE ELE L Invert image Play video from file F2 FS F8 Fil Settings 7 Ignore Inner Diameter F3 F6 F9 F12 Source Course Edge Detection Fine Edge Detection Inner Diameter vo _ Outer Diameter C Bad Sequencer not started F1 F2 F4 F5 F6 F7 FS F9 F10 Fil Fi2 Start 09 47 43 Figure 1 7 Image Analysis window moved and resized at the center of the MyoVIEW screen CHAPTER1 7 1 2 1 Reset of the MyoVIEW layout At any given time the layout can be set to default by going to the View Menu and click on the Reset window layout MyoVIEW for DMT Pressure Myograph Systems Show Data Sheet Time Secs 50 Export Type Mean Value Zone border color
28. r of choise e Select zone 1 or zone 2 and eventually adjust the y axis to see the recorded trace for the given parameter CHAPTER5 33 Scroll through the traces and recorded video by adjusting the time in the View Menu and by using the scrolling function See chapter 3 4 3 3 and figure 5 2 below The video of the experiment will be shown in the Camera Control window if Record Video was selected before the video capture was started Scrolling through a trace using the scroll function will show the trace param eter data as well as the recorded video image at the given time as seen in figure 5 2 below MyoVIEW for DMT Pressure Myograp s Zone 1 Outer Diameter 4 I Export Type Mean Value v Zone border color Reset window layout Show Action Sheet Start Live Trace I Export to Data Sheet Outer edge color Show Status Bar Select all traces IG Ve pand View Inner edge color Application layout Data Analysis Live Traces l Trace selection Zone colors bh m u X 2 A Action Sheet Zone 1 Inner Diameter Figure 5 2 Scrolling through the data using the scroling bar in the Live Traces submenu shows the traces as well as the corresponding video with the two zones Export the data of interest to the MyoVIEW Data Sheet as described in chapter 3 4 2 1 All the collected data in the MyoVIEW Data Sheet can be exported to a CSV or Excel Word WordPad The Raw Da
29. ransfer data from all open traces for the same time frame the Select all traces are selected When one time frame is selected in one trace all open traces will have the same time frame If this is followed by clicking on the Export to Datasheet in the Trace Selection submenu then the data from all the open traces for the selected time frame will be transferred to the Data Sheet To transfer only the data from one given trace and time frame the Select all traces is NOT selected When the Export to Datasheet in the Trace Selection submenu is selected only data from the selected trace and time frame is transferred to the data sheet Select all traces is marked Followed by Export to data Sheet will transfer data to the data sheet from all the selections shown in figure 3 28 28 MyoVIEW USER MANUAL Reset window layout Show Status Bar Application layout Figure 3 26 Application Layout submenu Show Data Sheet Show Action Sheet Select all traces Data Analysis Figure 3 27 Data Analysis submenu MyoVIEW for DMT Pressure Myograph Systems Zone 1 Inner Diameter Heater On Off Current Target 27 9 Change Figure 3 28 Selection of all traces See Data Sheet marked in figure 3 29 below MyoVIEW for DMT Pressure Myograph Systems Data Sheet Figure 3 29 Export of data sheet of all traces When Select all traces is NOT marked as shown below Export to data Sheet will only transfer data to the
30. sis Source Markers Zone 1 Inner Diameter x Action Sheet f 8 e mu Task Started Step 4 S0mmHoy m mm um um d I rask Started ste EE DE Been ITask Started Step 2 40mmHg Figure 2 28 Experiment using the sequencer The sequencer tasks are listed in the trace as marks 18 MyoVIEW USER MANUAL 2 6 Flow meter Control window optional The DMT FlowMeter 162FM is not an active flow meter able to control the flow The FlowMeter is only at the moment able to measure the flow as result of pressure gradient across the vessel due to the value for P1 and P2 Flow Meter nx DMT Flow Meter Flow pL min 15 Figure 2 29 DMT FlowMeter control window 2 7 DMT Inverted Microscope Control Window optional The DMT Microscope Control window shown below is used to adjust the Infrared Light IR light intensity in the DMT Inverted Microscope The IR light can also be adjusted directly on the DMT Inverted Microscope turning the silver knob on the back of the microscope The IR light can be used to obtain the optimal contrast image of the vessel to track the vessel walls DMT Microscope n x IR Light p Figure 2 30 DMT Microscope control window CHAPTER 2 19 CHAPTER 3 THE MYOVIEW MENUS AND CONFIGURATION 3 1 Application button DMT logo Figure 3 1 Application button New trace A new trace will be shown in MyoVIEW
31. ta can be exported to alternative programs for data analysis see below Export of raw data Export of raw data to Excel Word or WordPad Select mark a given part or the whole trace Drag the selected part to Excel Word WordPad The exported raw data will be shown in two columns where the first column always are the time and the second column the given parameter of the selected trace Export of raw data to a CSV file comma separated file Select mark a part of a trace or the whole trace Drag the selected part while holding down the ALT key to an appropriate folder The CSV file will be given an arbitrary file name Rename the file if wanted The exported raw data will be shown in two columns where the frist column always are the time and the second column the given parameter of the selected trace Export Video Image The image shown in the Camera Control window can be exported either as an image file bescribed in chapter 2 3 4 and as shown in figure 2 18 or drag the image in the Camera Control window to e g Word Powerpoint Excel Wordpad etc while pressing the SHIFT key 34 MyoVIEW USER MANUAL APPENDIX 1 PARAMETER FORMULAS USED IN MYOVIEW MyoVIEW performs several measurements and calculations relevant to vascular physiology These measurements and calcula tions are based on raw inputs data from the flow meter and pressure interface as well as vessel dimension outputs The follow ing is a list of the formulas used to calculat
32. ting tissue and fat attached making it impossible to track the right posi tion of the wall A Try to move the zone to another position B Use the Fine Edge Detection sliders in the Image Analysis Control window C Clean a new vessel and mount it in the Myograph chamber 2 3 3 Reset Fine Edge Dection sliders The Fine Edge Detection sliders can be reset by going to the Image Analysis control window and click on the camera and then select Reset Fine Edge Detection as shown in the figure 2 18 below 2 3 4 Snapshot image of the mounted vessel Go into the Image Analysis control window and click on the camera and select Export image to file as shown in the figure 2 18 below Image Analysis Camera T M a i i Export image to file i 4 Course Edge Detection 1 I Reset fine edge detection I s Y b me me e e e e m m m di Calibrate video Fine Edge Detection mee Stop capture Inner Diameter E t t 8 ad sla Outer Diameter d s eee 1 22 1 26 Record ee ing Sequencer not started F1 F2 F4 FS F6 F7 FS F9 F10 F11 F12 Figure 2 18 Reset Fine Edge Detection and Export image to file 2 4 Pressure Interface Control Window The Pressure Interface Control window is placed along the right side of the MyoVIEW layout as shown in the figure below The Pressure Interface Control window is divided into three parts Heater control Pressure Con
33. trol and Force control Pressure Interface ax Heater On Current Target 25 0 Gani Pressure On Current Target Pl 41 P2 4 6 Gradient Flow Force mN Current 0 3 Zero Force Figure 2 19 Pressure Interface control window 16 MyoVIEW USER MANUAL 2 4 1 Heater Control Heater On Current Target Control the temperature in the Myograph Chamber and Pressure Interface buffer bottles Enter the temperature of choice RT to 50 press ENTER on the keyboard or click the Change icon To turn the heat on and off click the On and Off icon respectively 2 21 Heater control 2 4 2 Pressure Control e Pressure On Off To pressurize the bottles in the Pressure Interface click on the On Pressure On icon and to turn off the pressure on the bottles in the Pressure Interface click on the Current Target Off icon P1 41 P2 46 NOTE Gradient WHEN MYOVIEW OPENS THE PRESSURE IS ALWAYS OFF AS A DEFAULT m e P1 and P2 Setting of the pressure at the P1 and P2 side of the chamber Enter the Figure 2 22 Pressure control pressure of choice at the P1 and P2 side 0 2bOmmHg and press the ENTER key or click the Change icon to save the entered values To prevent backflow MyoVIEW will not accept a higher P2 pressure than P1 pressure Gradient The Pressure Interface can build up pressure with different speeds The default setting is TOmmHg sec meaning going from 40mmHg to 50mmHg takes 1 sec The speed gradient can be chan
34. trol window CHAPTER2 17 Adding Task steps to the sequencer Sequencer Sequencer Add task Edit task Delete task 1 Click on the sequencer triangle icon and select Add task see figure 2 25 2 The Task manager see figure 2 26 n Several information are needed for each task to be filled in Claw mics Start Sequencer Image Analysis Loop Sequence Sequencer not started Description Free text describing the task Show description on trace when task starts If this is selected the free text from Description will be written on the traces when the task starts see figure 3 18 Duration Time in seconds the task should run before continuing to the next task Inflow P1 Target pressure in mmHg for the P1 0 250mmHg e Outflow P2 Target pressure in mmHg for the P2 0 250mmH Temperature C Chamber temperature RT 50 C Prompt user When the task ends the sequencer will prompt the user for an OK Figure 2 25 Sequencer drop down menu Description Step 3 60mmHg F Show description on trace when task starts with the text written into the text box before it will continue to the next task In figure M 2 26 the text Add Drug 1 have been added This results in a prompt box with the ind text Add Drug 1 that the user has to click OK before the sequencer continue to P next step ae e Wait A tick mark in the Wait box See figure 2 27 means that the task will con
35. us and configuration ernsnnnvevnnnnnnennnnnnnvennnnnnnnnnnnnnennnnnnvennnnnnnennnnnnnrennnnnnnnnnnnnvnnnnnnnnennnnnnnennnnnnnen 20 3 LApplicati n button DMT t 20 3 2 MYOVIEW NESNA 20 96 Mk E E EEE E A E ME N E A E A E A A RE MN A EE A E Re m 21 CEE MRS E U ay 29 SL MYO VIEW MONU EEE metn retin DNE ERICH NE EN MR NS 24 GERE ET SSE eg NS a a 24 FEN ende 24 IAL Markers SUBMENU e a RE EN Ne YI FIXE 24 GE ER EN ER VEN FM 24 TDN kile 26 TREE 27 3 42 2 Delete SMS U a a NN 2 9 2 2 2 Data Collection SUIS OE ES NE 27 S 2 VIGO Recording NNN 28 SIM OE VON NE T I T T S 26 3 4 3 1 Application Layout SUDMENU EE EN e a a Me 28 243 2 Data Analysis SUD EE NE EE 28 GEN CE LIVE Maces SUBMENU RITE tma 30 Mace Selection SU OS TOME o MN EEE NN ON 30 3 43 59 ZONE COIOFS SUD MENU EE TT ER a a a NM a 30 Chapter 4 Data Collection and Saving nanan amnanan manana 31 Chapter 5 Data Analysis and Export 5444 33 Appendix 1 Parameter formulas used in MyoVIEW sannrxnnnnnnvnnnnnnnnevnnnnnnvennnnnnrennnnnvennnnnnnnnnnnnnnennnnnnnennnnnnnennnnnnnennnnnnnennnnnnvnnnnnnn 35 Appendix 2 MyoView Upgrades or Change LICenSe sssrensnnvvnnnnvnnnnnvnnnnnvnnnnnnnnnnnvnnnnvennnnvennnnnennnnvennnnvennunvennnnvennnnvennnnvnnnnveennnneenn 37 NOIS R
36. used to scroll through the traces Press the minus to scroll back in time and the plus to scroll forward in time Alternative move the slider toward the minus or plus to scroll back and forth in time respectively 3 4 3 4 Trace selection submenu Export Type Selection of the data transformation type to be carried out on the se lected data Mean Value Calculate the mean value of the selected data Maximum Value The highest value in the selected data set Minimum Value The lowest value in the select data set Export to Data Sheet Transfer the Mean Maximum or Minimum value depending on the selected Export Type to the Data Sheet IMPORTANT BEFORE EXPORTING DATA TO THE DATA SHEET SELECT THE SELECT ALL TRACES IN THE DATA ANALYSIS MENU IF DATA FOR THE MARKED TIME FRAME FROM ALL OPEN TRACES SHOULD BE EXPORTED TO THE DATA SHEET OTHERWISE ONLY THE MARKED TRACE WILL BE EXPORTED Expand View Zoom function Select a time frame gt 5 sec and press EXPAND VIEW gt the selected time frame will be expanded to fill the whole viewing field of a trace e g a selected time frame of 90 sec will by pressing EXPAND VIEW result in traces where maximum the last 90 seconds of the traces can been seen on the screen at any given time When pressing EXPAND VIEW the selected time frame will be transferred to the Time field in Live Traces submenu 3 4 3 5 Zone colors sub menu 30 Zone Border color Select the color of the Zone box wherein the
37. v E1 EZ F10 F2 FS F8 F11 Settings F6 12 Markers Zone 1 None x 0 6 1 i hv Zonel None Left Wall Thickness Right Wall Thickness Average Wall Thickness Outer Diameter Inner Diameter Vessel Area Lumen Area Cross Sectional Area Media Lumen Ratio Shear Stress Inlet Pressure P1 Outlet Pressure P2 Mean Pressure Flow Vascular Resistance Temperature Force pH Figure 3 4 Select parameter from drop down menu Pressure Interface Heater Current Target Pressure Current Target P1 10 0 P2 9 9 Gradient Flow Force mN Current 0 0 off Zero Force Select the parameter of interest As many traces as requested can be opened and shown at the same time The traces can be stacked as tabs in columns or in rows The trace layout can easily be arranged as requested simply by dragging the actual Trace window to the requested position The three different layout are shown on the next pages in figure 3 6 3 8 If the data traces cannot be seen on the screen then first click on 1 for showing zone 1 or 2 if two zones have been made on the video image marked figure 3 5 below If the traces still can not be seen then the scale on the y axis could be set wrong Zone 1 Inner Diameter Figure 3 5 Click for zone 1 or 2 CHAPTER 3 21 AyoVIEW for DMT Pressure Myograph Systems Zone 1 In
38. vessel dimension should be measured Outer edge color Select the color of the tracking bar for the outside wall position of the mounted vessel Inner edge color Select the color of the tracking bar for the inside wall position of the mounted vessel MyoVIEW USER MANUAL Pressure Interface Heater Current Target 28 0 Pressure Current Target P1 10 1 P2 99 Gradient Flow Time Secs 50 Stop Live Trace QU Live Traces Figure 3 32 Live Trace submenu Export Type Export to Dal Mean Value Expand View Maximum Value Minimum Value Figure 3 33 Trace selection submenu Export type Export Type Mean Value v Export to Data Sheet Expand View Trace selection Figure 3 34 Trace selection submenu Zone border color Outer edge color Inner edge color Zone colors Figure 3 35 Zone colors submenu CHAPTER 4 DATA COLLECTION AND SAVING DATA MyoVIEW will not record and save data for any parameters before the Start recording data button is pressed at the status bar of MyoVIEW shown in figure 4 1 below MyoVIEW will in the traces write Data Not Saved as long as the Start button is not activated as an indication of that the incoming data will not be collected and saved Lyc ior BUT Pirmais n r jonsi mwar umae Figure 4 1 Start recording data button Pressing the Start button will reset the time to zero and the traces sho
39. wn and the Start button will change to a Stop collecting data button as showed in figure 4 2 below PA Vor DAT Kere lice fyr Poner enam Dame Figure 4 2 Stop collecting data button CHAPTER 4 31 The data will now be recorded and saved in MyoVIEW until the Stop recording data button is pressed When the Stop recording button is pressed a marker will be shown in the trace with the text Data Collection stopped and in the Action Sheet see figure XX a line will be entered with the text Data Collection stopped with a time stamp Furthermore all traces will show the text Data Not Saved when the Stop button has been pressed or the Start button not activated If the Start collecting data button is activated again after it has been stopped using the Stop button MyoVIEW will ask if the former recorded data should be saved in a file or discarded AT Existing experiment data will be overwritten Save before starting new capture Figure 4 3 Warning message save If the data should be saved enter a title of the experiment and a description of the experiment as shown in figure 4 4 below Press save Mesenteric artery from Balb c mouse Figure 4 4 Save Experiment Enter a filename and the location on the computer where the file should be saved After reactivating the Start button and saving the former data MyoVIEW will zero the time reset the trace and start collecting and saving the new data 32 MyoVIEW USER MANUAL CHAPT
40. y on the computer should be considered before selecting the quality of the recorded video 3 4 3 View Menu 7 Record Video Video Quality Medium Figure 3 24 Video Recording submenu In the View Menu there are five submenus Application layout Data Analysis Live Traces Trace Selection and Zone Colors MyoVIEW for DMT Pressure My ograph Systems MyoVIEW Data View Show Analysis Window Show Data Sheet Reset window layout Show Action Sheet M Show Status Bar Application layout Time Secs 50 Export Type Mean Value Export to Data Sheet amp Expand View Trace selection Stop Live Trace QU Live Traces Select all traces Data Analysis Zone border color Outer edge color Inner edge color Zone colors Figure 3 25 View Menu 3 4 3 1 Application Layout submenu Show Analysis Window Will open up the Image Analysis window Video from cam era if closed by the user Reset Window Layout Return the MyoVIEW layout to the default layout Very useful in situations where the layout needs to be cleaned up Show Status Bar The status bar is the bar at the bottom of the screen showing the Hotkey s F1 F12 3 4 3 2 Data Analysis sub menu e Show Data Sheet Open the Data sheet Show Action Sheet Open the Action Sheet The action sheet will list all the events as e g camera calibration sequencer tasks markers etc Select all traces To t
41. yoVIEW start screen without DMT microscope attached 1 2 Layout The layout on the screen can be simplified by hiding the different small windows for the DMT Inverted Microscope or video from normal inverted microscope e g Zeiss Camera Pressure Interface Image Analysis Sequencer and Flow Meter optional By clicking the needle pin icon in the top right hand corner marked in figure 1 3 below the windows will hide either on the left or right hand side of the MyoVIEW screen Figure 1 3 Indication of needle pin icon CHAPTER1 5 After hiding the five different windows the MyoVIEW layout will look like this be tee DMT rata Figure 1 4 Layout after hiding the windows The five different windows will slide in if requested and be visible by holding the mouse pointer on the yellow tab By pressing the needle pin again the window will stay open and not be hidden see figure 1 5 A B below Vertical needle pin The Camera Menu will NOT be hidden Horizontal needle pin The Camera Menu will be hidden Figure 1 5 A B Indication of vertical needle pin icon left and horizontal needle pin icon right The Image Analysis window showing the video from the USB camera can be moved around and resized as required Simply hold ing down the left mouse button on the Image Analysis window and drag it to the requested position on the screen At the given position the size of the Image Analysis window can be scaled u
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