Lab-Scale Electrospinning Unit (Electroris) user`s mannual 2013
Contents
1. 3 810 mor 0 015 0 381 pem 8 0 165 4191 0 135 3 429 9 0 148 3 759 0 118 2 997 10 0134 3404 0 106 2 692 0 014 0 356 11 0120 3 048 0 094 2 388 0 013 0 330 12 0 109 2 769 0 085 2 159 0 012 0 305 13 0 095 2 413 0 071 1 803 14 0 083 2 108 0 063 1 600 0 0 0 254 15 0072 1829 e 0 054 1 372 PUMA 0 009 0 229 oS 16 0 065 1 651 0 047 1 194 17 0 058 1473 0 042 1 067 0 008 0 203 18 0 050 1270 0 033 0 838 0 0085 0 216 19 0 0422 1 067 0 027 0 686 0 0075 0 191 20 0 03575 0 9081 aUe 0 02375 0 603 er 0 006 0 1524 aoe 21 0 03225 0 8192 0 02025 0 514 22 0 02825 0 7176 0 01625 0 413 22s 0 006 0 152 0 0111 0 2826 23 0 02525 0 6414 0 01325 0 337 0 006 0 1524 24 0 02225 0 5652 0 01225 0 311 0 005 0 1270 25 0 02025 0 5144 0 01025 0 260 26 0 01825 0 4636 0 004 0 1016 26s 0 01865 0 4737 0 005 0 127 0 0068 0 1734 27 0 01625 0 4128 0 00825 0 210 0 004 0 1016 28 0 01425 0 3620 9 00725 0 184 0 0035 0 0889 29 0 01325 0 3366 0 003 0 0762 30 0 01225 0 3112 0 00625 0 159 31 0 01025 0 2604 0 00525 0 133 0 0025 0 0635 32 0 00925 0 2350 0 00425 0 108 33 0 00825 0 2096 0 002 0 0508 34 0 00725 0 1842 0 00325 0 0826 eo2. Fnm Co temperature Hence a small ventilation fan is placed behind the device It 1s controlled by a thermostat timer and is automatically turned on off Description If it is necessary to work at room temperature it s better the desired temperature is set below the room temperature rather than turning off the heating system that makes the ventilation fan turn off Set mode Press key for minimum 3 seconds The electroris is set in beow values For more information about digital thermostat see appendix A Lightening the chamber You can turn on the chamber light by using the key below High Voltage Power Supply HVPS The power supply embedded into the device has a positive 35 kV output Two different types of HVPS are available in this system OV Series with an output high voltage indicator Accuracy 0 1 kV OC series with an output high voltage indicator Accuracy 0 1 kV and an output current display Accuracy 1 micro amp 19 Lab scale Electrospinning unit Electroris user s mannual Fnm Co There is a 10 turn potentiometer as a voltage regulator to increase or reduce the voltage from 0 to rated voltage Turning on the HVPS Use the On Off key on the right of HVPS Note For more safety before turning on the HV set the potentiometer at a low voltage and then adjust 1t at the desired value In this case any unwanted connection will be identified and fixed at lower voltages Kilo Voltm
3. more than one syringe can alter the nanofiber size and its morphology So it s better to use one syringe to optimize the process If you use more than two syringes the minimum distance between two nozzles should be about 4 cm more or less although this distance depends on the polymer voltage and etc If you need more than two syringes please contact with Fnm Co By pressing the key L1 the following message will be shown In this page 3 injection parameters are adjustable Second Row L2 syringe inner diameter It s the most important parameter in calculating the flow rate so it s necessary to measure and enter it correctly When the syringe is changed the new diameter value must be set Units are in millimeters mm Enter the inside diameter ID of the syringe you wish to use If you do not know your syringe diameter refer to appendix A for nominal inside diameters of most popular syringes For the greatest accuracy or if your syringe is not listed in appendix B measure the inside diameter with a vernier caliper or other precision measuring tool Record this value for future use For setting it press the key L2 until the registered number blinks Then enter the new value by using the panel numeric keys 00006000000 13 Lab scale Electrospinning unit Electroris user s mannual Fnm Co Register or cancel the new value For removing the last entered number you can use the backspace key In o
4. not occur if it does droplets are electrosprayed and a charged liquid jet is formed As the jet dries in flight the mode of current flow changes from ohmic to convective as the charge migrates to the surface of the fiber The jet is then elongated by a whipping process caused by electrostatic repulsion initiated at small bends in the fiber until it is finally deposited on the grounded collector The elongation and thinning of the fiber resulting from this bending instability leads to the formation of uniform fibers with nanometer scale diameters The standard laboratory setup for electrospinning consists of a spinneret typically a syringe needle connected to a high voltage 5 to 50 kV direct current power supply a syringe pump and a grounded collector A polymer solution sol gel or particulate suspension 1s loaded into the syringe and this liquid is extruded from the needle tip at a constant rate by a syringe pump Syringe pump The syringe pump of this device has been designed in order to use of a variety of syringes The system is able to inject the certain volume of solution with different rates All models Lab scale Electrospinning unit Electroris user s mannual Fnm Co employ a microcontroller which controls a small step angle stepping motor that drives a lead screw and Pusher Block Micro stepping techniques are employed to further reduce the step angle eliminating flow pulsation A keypad is used for entering the data to the pum
5. position Fourth Row L4 Scan rate mm min Example 1 StartPos 100 EndPos 250 Speed 500 According to the example the scanning is done from the starting point 100mm to 250mm with the rate of 500 mm min 15 Lab scale Electrospinning unit Electroris user s mannual Fnm Co Example 2 StartPos 150 EndPos 150 Speed 0 According to the example only adjusting the nozzle is done at the point of 150mm and remains constant Distance adjustment system Distance between the nozzle tip and the collector is called electrospinning distance Due to the conditions it is usually from 10 to 20 cm more or less In most systems the collector is grounded however connecting the negative voltage to the collector has interesting results Note According to different sizes of nozzles the electrospinning distance can be more less than the calculated value so it must be corrected by the user By pressing the key L3 the following message will be shown D1sLance Distance 54 It has only one adjustable parameter Third line L3 The distance adjustment previous data change to blink mode and new data is requested In order to enter new data you have to use the keys O090 6000009090 then press the key L3 again to record the new data Note After entering the new data the previous data remain until the key L3 1s pressed The drum rotation speed Due to the type of electrospinning s
6. the Electroris package are Power cable User manual PVC hose Earth cable and Fuse In software models software CD and USB cable 1s also included Installation Physical installation The following conditions must be noticed at the place where you work A dry clean and hard surface for keeping the instrument Minimum of about 15 cm clearance around the device suitable environmental conditions a proper ventilation Turning on the device e Plug in the power and then connect it to the input voltage of Electroris Turn on main power switch located on the rear of device Use black key on the right side of device to turning on the Electronic control section The display will now illuminate indicating that the power connections are correct The display will indicate FNM Co internet address for 3 seconds Then the scan system will be activated and the nozzle will be set at zero After that the distance axis will set the drum at a 5cm distance during this time the term Set Zero will be shown on the display e Use red key on the right side of device to turning on the High voltage power supply section At this moment the high voltage power supply is in standby mode that is shown by the blue LED e In this step the device is ready to use Lab scale Electrospinning unit Electroris user s mannual Fnm Co 4 1 4 2 4 3 4 4 Syringe 4 5 Pusher Blocks Syringe Clamp 4 6 Stepper motor case thumbscrew 4 7 Plastic case Clamp S
7. with other systems connection with defective equipments voltage fluctuations fire or intensive heat water or corrosive chemicals dust lightning natural disasters collision misusing the instrument or neglecting the manual description e The devices that have been repaired by other companies e The devices that have been manipulated e Damaging the instrument labels or holograms e Damage resulting from installation or updating the files software program and firmware by irresponsible people e Damage resulting from not connecting the device to the earth e Damage resulting from connecting the high voltage wire to the body of device drum syring pump and etc According to the warranty our company will repair or replace the defective device free of charge You can send us the defective part s of device such as HV power supply or and syringe pump by post We will accept any charge made for posting Lab scale Electrospinning unit Electroris user s mannual Fnm Co Repair Facilities and Parts Fnm Co stocks replacement and repair parts While ordering please describe parts as completely as possible Preferably by using the part numbers a sample or drawing CAUTION Electroris use High voltage power supply for generating nanofibers The term high voltage usually means electrical energy at voltages high enough to inflict harm or death upon living things High voltage can bridge long distances and break through the isolation On th
8. GOC and Operational keys Syringe Table and Custom Syringes If a non standard syringe is to be used enter the inside diameter of the syringe in millimeters Syringes are arranged according to manufacturer and material and then according to size See Appendix A Target Volume Specify the volume that is to be infused The pump will run at the rate specified until this volume has been delivered in the Volume mode Nonvolatile Memory All operational data entered into the pump from the keypad or from a computer will be stored including the program Smooth injection Using a high accuracy motor and driver profiles deliver very smooth and consistent flow that is virtually pulse free Adjusting the Pusher Block Using two keys that move the location of pusher block forward or backward can cause adjusting of its position rapidly High Voltage Cable Before turning on the device make sure that the cable is connected to nozzle s Connecting the high voltage cable to every part of the device except the nozzle causes transmission of high voltage to the device and can fail it Also it can damage the high voltage power supply Note Before starting the machine be sure that the high voltage power supply is off Visual Audible Alarm In addition to visual alarm devices the device has an audio alarm that will sound in required times Lab scale Electrospinning unit Electroris user s mannual Fnm Co Package contents Contents of
9. HGCMING KEE GhenMOC hic scvactahs a a de del ita a de oo lo der al oda 19 High Voltage Power Supply UHV PO essa dsd iria 19 Appendix A Syringe inside Diameter ooooccccccccccccnnnnccccccanncncn nmm rr rra narran e Appendix B Set mode of Digital Temperature Controller seeeeeeeeesee 22 Appendix C Syringe Needle Conversion Chart essesrussrrerrrerrerrrerrrerrerrrrrrrrrrerrrre 23 Lab scale Electrospinning unit Electroris user s mannual Fnm Co General Information Serial Number All inquiries concerning our product should refer to the serial number of the unit Serial numbers are located on the rear of the case Calibration All electrospinning units are designed and manufactured to meet their performance specifications at all rated voltages and frequencies It s necessary to calibrate the instrument every 6 month It s suggested that choose a 10 mililitre syringe and a 1 mililitre target then measure the amount of pedal pusher block movement forwards in 1 6 and 60 ml h The movement must be 12 73 mm To measure the accuracy of internal timer the injection time must be 60 10 and 1 minute s Warranty Fnm Electroris is warranted for a period of one year from date of purchasing At its option Fnm Co will repair or replace the unit if it is found to be defective as to workmanship or material The warranty does not extend to e Damage resulting from transportation wrong connections
10. Lab scale Electrospinning unit Electroris user s mannual Fnm Co SEI yht Fanavaran Nano Meghyas Lab Scale Electrospinning Unit Electroris user s mannual 2013 General INtormMaciON meandro ca 3 Est AP CIO 3 Warranty POPPCOOO COn RANA 3 Repar Facilities ana POTES A E N 4 Grounding neo vstem Estonian EA 4 GENERAL SAFETY SUMMAR arta ii A TC EFE DOT DEA U2 45 4 De zac aa cin EAS iia did dis 6 Hem CIN eo Nes COPAN CON sate iat a o A oan odensaeenane ame 6 als a a AAA SA 6 ElESTPOSPINTINA Fr rOCE S Sidra idad 6 SVCINGS UI O ed 6 evo tee et he tere re UT TUTUP 8 Syringe able a a Uso OV NGE S scmper ERE COR EH E E AAA 8 Bells cae OUO eaae UE E eee ere eee eer 8 NGHVOlSCHE MENO Viners orr T eo p NS 8 MOGET ACE EO E O 8 POSING ee Pusner BIOOK stor HE in ote a eO inus obtu mulo tiug tive APO 2 TENES 8 PIG voltage Cable recreire in rinda dd 8 Visual Audible Alarm aa a o wr C RA 9 MSM ION Lc IM MI ena 9 Bid SiG ait mcis UR Io IUUD 9 Turning lana he iz APARATO 9 Loading the SyrINQR ccccccccccnnnccccccnnnnnn nn rr rn nn 10 PrODEr SVCINGe Selec ia 11 ISI TIT T ILI eee ee Or 11 Woerd er IE E a NS ius toa poate TRIN Ena 11 DEB ENC y KONS us Lm EOS 12 OVringe pump Unjection SS TEM A ERR RC a o 12 NOZIO OCN Li ara 15 Distance adjustment SISTE xiscccceiaaeepevetaue isis 16 The Crum cotation SPECO uta data praia 16 setting the temperature of COaMber coccccccccccnccccccnnncnnnnnnnnnnnnnncc rr ran nr 18 Se MOE Ara 19 LAG
11. Note the target must always set at a suitable value Clean the instrument Clean the keyboard and body of the instrument with a medium detergent Oiling Oil the syringe pump screws and their connections in certain time Nozzle Cleaning After finishing the work clean inside of nozzle and connections completely put a piece of thin wire inside the nozzle in order to avoid drying polymeric solution O Lab scale Electrospinning unit Electroris user s mannual Fnm Co Observe all Warning Labels on Product Read all labels on product to ensure proper usage Using an organic solvent Notice that the used hose is able to solve in some organic solvents so use Teflon or Polyethylene hose instead of PVC Please inform FNM Co staff if there is any problem The instrument limits Accuracy largely depends on the syringe and its diameter Measurement error will directly cause the injection error Warning The sound alarm inside the device can be set up to warn the user when completing each order Introduction Theory of Operation Electrospinning Process When a sufficiently high voltage is applied to a liquid droplet the body of the liquid becomes charged and electrostatic repulsion counteracts the surface tension and the droplet is stretched at a critical point a stream of liquid erupts from the surface This point of eruption 1s known as the Taylor cone If the molecular cohesion of the liquid 1s sufficiently high stream breakup does
12. Prevent Hazard or Injury Use Proper Line Cord Use only the specified line cord for this product and make sure line cord is certified for country of use Use Proper Power Supply The unit 1s supplied with an approved power supply and line cord To maintain the safety integrity of the device use only one of the following power supplies e 200 240 V AC 50 60 Hz Ground the Product This product is grounded through the grounding conductor of the power cord To avoid electric shock the grounding conductor must be connected to earth ground Before making any connections to the input or output terminals of the product ensure that the product 1s properly grounded Make Proper Connections Make sure all connections are made properly and securely Use Proper Fuse Use only specified fuses with product Avoid Exposed Circuitry Do not touch any electronic circuitry inside of the product Do Not Operate with Suspected Failures If damage is suspected on or to the device do not operate it Contact with qualified service personnel Place the device in Proper Environment Review the operating manual for guidelines for proper operating environments Keep the instrument in a dry clean and flat place Avoid connecting the ventilation fan near the walls High viscosity solutions Don t use high viscosity solutions and syringes with very low diameter nozzle High gauges size Finishing the solution Switch off the system after finishing the solution
13. Syringe pump injection system The syringe pump can take one or two syringes make by nonconductive materials plastic or glass syringes and sizes below 50ml The inner diameter of the syringe s is entered by the keyboard and the internal microprocessor calculates the cross sectional area to calibrate the pump for that syringe Note 1 Please be careful the hose is not soluble in the solvent For example PVC hose can be solved in most organic solvents In the case use Teflon or polyethylene hose Note 2 At the end of electrospinning process it 1s necessary to clean inside the nozzle s and tube Then put a piece of thin wire inside the nozzle s to avoid drying the polymeric solution Note 3 The electricity can be transferred to the syringe through the tube so the syringe shouldn t be contaminated by the solution If the syringe is contaminated you will hear the discharge noise In this time should be turned off the high voltage and cleaned off the conaminated area immediately 12 Lab scale Electrospinning unit Electroris user s mannual Fnm Co Note 4 Most existing tubes are not strength enough to avoid passing high voltage electricity so it s necessary to keep tubes far away from devices such as syringe pumps In the syringe pump one or two syringes can be used Using two syringes increases the amount of produced nanofiber However because of interaction between the formed polymer jets on the nozzles tip using
14. crew Loading the Syringe l Aea a Adjust the syringe pusher block 4 5 by pressing the positional switches D c on the keypad Raise the spring loaded syringe retainer 4 3 and swing it out of the way Lay the loaded syringe in the V shaped syringe block Swing the syringe retainer so it holds the syringe in place Move the pusher block 4 5 using the positional switches so it makes contact with the syringe plunger Tighten down thumbscrews on syringe block bracket so that it captures flanges on syringe barrel Tighten thumbscrew into place when switch is set 10 Lab scale Electrospinning unit Electroris user s mannual Fnm Co Proper syringe selection Min Rate Microliter hour 0 5 Syringe diameter mm 2 Max Rate Mililiter hour 0 80 Syringe diameter mm 2 Example a Imm diameter syringe Min rate 144 5 ul h Max rate 231 2 ml h a 10mm diameter syringe Min rate 50 ul h Max rate 80 ml h a 17mm diameter syringe Min rate 50 ul h Max rate 80 ml h Display System injected 4 868 ml Scan Pos istance 58 umi OFF This screen consists of four rows lines that show the situation of each sub system First line the injected volume of solution ml Second line The position of nozzle s per millimeter Third line The distance from the nozzle tip to the drum surface mm Fourth line Drum rotation speed based on RPM Description 1 The keys L1 L2 L3 and L4 located on
15. e surrounding objects it can form a dangerous charge If not properly connected to proper discharge resistor capacitors can remain charged even long after shutdown Sparks 1n air The dielectric breakdown strength of dry air at Standard Temperature and Pressure STP between spherical electrodes is approximately 33 kV cm This 1s only as a rough guide since the actual breakdown voltage 1s highly dependent upon the electrode shape and size Strong electric fields from high voltages applied to small or pointed conductors often produce violet colored corona discharges in air as well as visible sparks Grounding the System Earth Due to the high voltages required to form the polymeric nanofibres it 1s necessary to ground the device through a grounding conductor of the power cord Lack of a proper earth connection and electronic system failure can carry electrical shock risks to the operator The screw at the back of device can be connected to the water metal pipe of the laboratory by a piece of wire Maintenance and General Safety Summary GENERAL SAFETY SUMMARY Please read the following safety precautions to ensure proper use of your device To avoid potential hazards and product damage use this product only as instructed in this manual If the equipment 1s used 1n a manner not specified by the manufacturer the protection provided by the equipment may be impaired Lab scale Electrospinning unit Electroris user s mannual Fnm Co To
16. eter accuracy 0 1 kV 100 V For more safety there are switch on the each door of instrument that cause to stop the HV when one of the doors is open Note If HVPS is on but the Kilo Voltmeter shows zero voltage check the doors to be closed Micro amperemeter in OC series There is a micro amperemeter to show the current at the high voltage output that provides interesting information about electrospinning process Note Due to low electricity consumption in the electrospinning process any increasing in the current shows the discharge In this case the device should be turned off and the discharge situation especially connection sites in the syringe pump should be checked 20 Lab scale Electrospinning unit Electroris user s mannual Appendix A Syringe inside Diameter Terumo Size Diameter 3cc 8 95mm 5 13 00 10 15 80 20 20 15 30 23 10 60 29 10 Sherwood Monoject Plastic Size Diameter 1cc 465mm 3 8 94 6 12 70 12 15 90 20 20 40 do 23 80 60 26 60 140 38 40 Popper e Sons Inc Perfektum Glass Size Diameter 0 25 cc3 45 mm 0 5 3 45 1 4 50 2 8 92 3 8 99 92 11 70 10 14 70 20 19 58 30 22 70 50 29 00 100 35 70 Stainless Steel Size Diameter 8cc 9 525 mm 20cc 19 130 50 cc 28 600 100 cc 34 900 Becton Dickinson Plastic Plasticpak Size Diameter tcc 4 78mm 3 8 66 5 12 06 10 14 50 20 19 13 30 21 70 50 60 26 70 Air Tite All Plastic Size Diameter 2 5cc 9 60 m
17. imit 14 Lab scale Electrospinning unit Electroris user s mannual Fnm Co Continuous infusion In order to continue the infusion the volume should be set at zero Cancel Reset the injected value By pressing the key MA twice causes the volume is set at zero Finishing the solution In some models when the pedal Pusher Block reaches to the end position the system will automatically stop in order to avoid damaging to screws and the motor so the pedal must be moved backwards by the positional keys and the syringe have to be changed Note The most of models don t have this switch so be careful of finishing the solution Note Because of different sizes of syringes automatically determining the end of injection is difficult So it is recommended that the maximum volume L4 is set at the amount of solution For example if the syringe contains 2 mililiters of the solution the maximum volume should be set at 2 to turn the device off automatically after finishing the solution Nozzle Scan System By pressing the key L2 the following message will be shown La E BLartPo wiv EndPos ol BE i nii gt Peed 14644 Hrmmmir In this page 3 parameters are adjustable Second Row L2 Scan start position per mm Adjust the starting point of scan It cannot be more than end position Third line L3 Scan end position per mm Adjust the ending point of scan It cannot be less than start
18. m 50 12 45 10 15 90 20 20 05 30 22 50 50 29 00 Unimetrics Series 4000 amp 5000 Size Diameter 10 ul 0 460 mm 25 0 729 50 1 031 100 1 460 250 2 300 500 3 260 1000 4 610 e Fnm Co SGE Scientific Glass Engineering Size Diameter 25yl 0 73 mm 50 1 03 100 1 46 250 2 30 500 3 26 1 0 ml 4 61 mm 25 7 28 5 10 30 10 14 57 Hamilton Microliter Series Gastight Size 5 pl 1 2 5 10 25 50 100 250 500 1 0 ml 2 5 5 10 25 50 Diameter 0 103 mm 0 1457 0 206 0 3257 0 460 0 729 1 031 1 46 2 3 3 26 4 61 mm 7 28 10 3 14 57 23 0 32 6 Lab scale Electrospinning unit Electroris user s mannual Fnm Co Appendix B Set mode of Digital Temperature Controller x A 1 C Display Mode trSL 1 B 0 1 C Display Mode trSL 0 9 Press key Y for Min 3 sec NY 3 Input compensation 0 EN 0 Control method setting Ot JP o O A Caution Compensation for accidential l o JAS error and le of sensor Set Value Default 0 Initial set value display preservation j 0 0 y L 5 H 4 Set range Limitation upper limit Cooling Mode Heating Mode A TSL Lowest limit 150 caaL H E H L 8 TSL Lowest limit 150 0 _ Default 100 0 Default cool 2 N Default 90 0 Set Value y preservation a EN MT SetValue preservation _ Pi U 5 L 5 5 Set range Limitation Lowest limit 1 Variation setting L TSH upper li
19. mit Variation setting o B 50 0 TSH upper limit o B 02 500 Set Value f Default 40 0 Default 1 0 preservation Set Value Se preservation i 2 Delay time setting 6 Auxiliary output selection Pal d b5Ha Default 0 OQ o Delay Timo Setting 0 240 o o L Default 0 sec Set Value preservation i j A OG b gt U i y i 7 Alarm t rature control 7 ON Time setting Alarm temperature control Time ON time setting A 0 O ojo OlO ow 50 0 150 Default 40 pua pa Default 1 min Set Value preservation 3 y Set Value preservation 8 Variation setting for Alarm BAJE 2 d ARE F eaf BtaF OFF time setting oF Mn avg for Alarm o O Time OFF time setting B 0 2 50 0 woe d Default 1 0 LN Vu SOME Sm Set Value preservation e Set Value preservation 9 Delay time setting for Alarm q A d i 9 Main output control L Ino E Delay time setting for Alarm Q Q 0 Cancellation 0 240 sec 1 Output control Default 0 sec ae N gm Default 0 Set Value preservation 6 bi 9 Set Value preservation NE y ec Lab scale Electrospinning unit Electroris user s mannual Fnm Co Appendix C Syringe Needle Conversion Chart Needle Nominal Outer Diameter Nominal I nner Diameter Nominal Wall Thickness Gauge inches mm en inches mm FOR Nee inches mm ce mm mm mm 7 0 180 4 572 Los 0 150
20. p The diameter of the syringes is entered via the keypad and the internal microprocessor drives a precision stepper motor to produce accurate fluid flow The pump can hold syringes of nonconductive materials plastic or glass make from about 1 ul to 50ml Nonvolatile memory stores the last syringe diameter and flow rate along with other configuration data Front of Electroris 1 HVPS 2 Drum collector 3 System controller amp panel 4 Syringe pump Features Bright Display and Easy To Use Interface Using a 4 row 20 character LCD display cause to show the essential information and its background light facilitates the reading of information in the darkness Using 20 keys avoids the need for a multi menu so the system will be set and will start to operate by pressing a few keys Different parameters such as flow rate syringe diameter maximum of injection start amp end scan position scan speed and distance are adjustable A functional key for each line of LCD gives easy access to sub systems of Electroris It will be discussed 1n detail later Informational messages will be shown sometimes to indicate the setting of data out of range or a problem Pressing any key clears the message from the display Lab scale Electrospinning unit Electroris user s mannual Fnm Co Keypad The keypad consists of 20 membrane keys These keys are grouped into 3 sections functional keys L1 L2 L3 and L4 Data Entering keys OOOOOSGOSGe
21. rder to save the value press just related line switch For example the key L2 should be pressed to record the syringe diameter To withdraw from the new value you have to press Cancel on the key So the previous value will be shown again Nonstop injection In some versions it must be to set Target in 0 to have a nonstop injection Note Pressing the key causes cancel or return to the higher stage Third line L3 Injection rate Flow rate Infuse rate milliliter per hour it indicates the injected volume in one hour Minimum and maximum injection rate According to the selected syringe the maximum and minimum injection rate can be vary It s suggested using a 5 ml syringe for rates less than 10 ml h or a 2 ml syringe for rates less than 1 ml h to increase the injection accuracy For higher rates you have to use a syringe larger than 5 ml Description The minimum and maximum rates largely depend on the syringe diameter If the entered injection rate is more than the defined rate for the system the message below will be shown So you have to use another syringe for your application Maximum sPFeed i 692 7 MM Fourth Row L4 Maximum injection volume ml If you want to stop the system after certain injecting volume of the solution you can use this section In this case after injecting the specified volume the system will stop and the following message will be shown InJecteg Uolune Lo max l
22. tem On Off m es HA YOUNG e l A Because the temperature can directly affect the viscosity and surface tension of polymeric solutions it is one of the most important parameters in electrospinning process especially in research work By pressing the key gt the desired temperature of chamber will be set In this case the keys Up and down can be used in order to increase or reduce the temperature e F control ProF 1 ON OFF control Temperature XM x we Current set up SV Default 25 0 Temperature Change of S V Qm Q Set Value gt preservation A g f xd Current Temperature Note 1 The maximum adjustable temperature is 45 C Although it s possible to be more than 45 C it 1s recommended to set the temperature below 45 C to avoid damaging the electronic parts and power supply system iP Fu nction Selection Changa of S V Note 2 In this system you cannot set the temperature of chamber less than the ambient temperature Ventilators Since the formation of nanofibres is done by evaporating the polymer solvent any factor that reduces the evaporation can impair the formation of nanofibres Because the chamber is closed after a short time the solvent vapor saturates the chamber and the evaporation process will be slow So it 1s necessary to bring the solvent out of the chamber without affecting the 18 Lab scale Electrospinning unit Electroris user s mannual
23. the left of each row are used to set each sub system Description 2 The last character on the right of each row indicates the moving gt or stop situation User Interface The user interface consists of a keypad with a display area The panel consists of a 4 row 20 character alphanumeric vacuum green or blue fluorescent display a 10 round volume drum rotation speed control a system to control the chamber temperature two LEDs blue and red and a switch to turn on and off the fluorescent lamps 11 Lab scale Electrospinning unit Electroris user s mannual Fnm Co Heater Fan Programmable Electrospinning Unit Faneveran Mi dad Nano Meghuas The Keypad of Fnm electrospinning system Setting displays are used to facilitate entering data into the system software Data entry will be discussed in detail later in this section Informational messages occur at various times to indicate such items as a data setting out of range or a detected problem such as the pump out of range flow rate Pressing any key clears the message from the display DESCRIPTION OF KEYS The keypad consists of 20 keys The keys are used for entering control information and data into the device Data Entry Keys 00000000000 Used to enter numeric data values or access special features ENTER Saves and stores displayed data value in memory when setting a data item Cancel Cancel the new displayed data and return the stored value
24. ystem the minimum and maximum drum rotation speed can be variable In high speed systems up to 2500 rpm the minimum rotation speed is about 350 rpm 16 Lab scale Electrospinning unit Electroris user s mannual Fnm Co Fourth line L4 By pressing the key L4 the OFF will be zero Then by pressing the fr Run button the drum will begin to rotate and the rotation speed 1s shown in the fourth line In this page The injected volume is 0 127 ml 127 microliters the scan position of nozzle is 198 mm the electrospinning distance is 50 mm fixed and the drum speed is 2788 rpm Adjusting the drum rotation speed A 10 turn potentiometer has been placed on the left of the panel in order to increase or reduce the rotating speed of drum Max Drum Speed Note If volume is set at low levels due to the low voltage applied to the drum motor when the system starts the drum won t begin to rotate It 1s better to set the potentiometer in 3 and if it s necessary adjust the rotation speed after starting If you want to cancel the drum rotation press the key L4 and put it in OFF mode 17 Lab scale Electrospinning unit Electroris user s mannual Fnm Co Setting the temperature of chamber The maximum temperature of chamber has been set 45 C The red LED indicates that the heating system 1s ON and the blue LED shows that the ventilation system 1s ON The right key 1s used to turn this sys
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