Omega Lum G User Manual
Contents
1. UV Safety Switch The UV safety interlock switch is located on the inner door frame Opening the door will trip the safety switch and turn off the UV transilluminator if it is in use Always exercise caution when working with UV For more information see Appendix A www gelcompany com System Setup UV Override Remember to observe all standard UV safety precautions when overriding the UV Safety Switch These include but are not limited to UV protective face Shield gloves lab coat etc To visualize or excise bands you may override the UV safety switch with the following steps e Open the door pull out the UV tray and position your sample e Select your desired UV wavelength e Pull out the UV switch e n the software select the EtBr UV 302nm application agingStudio Gallery e Select the Live button The UV light will come on When finished EtBr UV 302nm select Live again to turn off the UV light www gelcompany com TTT Software Introduction The Omega Lum Image Capture Software comes pre installed on the system tablet The capture software controls the light sources and image capture The separate UltraQuant analysis software is included for easy image annotation and analysis on your laptop or PC The Gel Company USB drive in the accessory package contains a copy of the analysis software three analysis software registration keys copies of the user2. e Close the filter shield www gelcompany com Appendix D Routine Maintenance e Select the Settings Tab Enter the name of the filter in the m correct location Ee inne User Management Position Display Name 1 Chemi 2 Orange 590 50 escis 3 Filter 3 empty 4 Filter 4 empty 5 Filter 5 empty Residual Bulk Image 6 Filter 6 empty Filter Wheel Filter 3 empty Home Edit Emission Filters E mail Settings x Save Changes e To replace a filter e Select an application with the filter in question e Select Capture to move the filter into place e Open the filter wheel shield e Remove the old filter and screw in the new filter e Close the filter shield www gelcompany com 33 4 Appendix D Routine Maintenance Replacing UV bulbs The UV bulbs can be replaced without removing the UVT from the system e Open the door e Pull out the UVT e Remove the 4 screws 2 on each side from the UV glass assembly e Lift the UV glass assembly off to reveal the bulbs e Using a pair of needle nose pliers with grip as an aid rotate the bulb and lift it out of the ballast housing e Replace the bulb and rotate the bulb to the locked position e Replace the UV glass assembly and screws www gelcompany com c O _ gt Q O D op z z z gm g compa
3. e Turn on the Tablet Power The tablet power is located at the top of the tablet Press and hold the button to turn on the tablet power e Launch the Software At the home screen select the Omega Lum tile The software will launch and you are now ready to use the Omega Lum system www gelcompany com System Setup Adjust Tilt of the Tablet PC To change the angle of the tablet PC hold the tablet frame with one hand and loosen the locking knob with you other hand Adjust the tilt to the desired angle and retighten the locking knob UV Transilluminator and Pull Out Tray The Omega Lum System has a dual wavelenght UV Transilluminator UVT on an integrated pull out tray The UVT can be used to visualize and cut out bands for downstream analysis To pull out the tray e Open the door e Use the handle to pull the tray out along the slides e Use the 302 365 switch to select the desired wavelenght Additional Sample Trays The Omega Lum System comes with the following Sample Trays e Chemi Tray This flat black tray is used for chemi and or fluorescent western samples The tray can be placed on top of the UVT or on the elevated tray slides for increased resolution e White Light Conversion Screen This flat white tray can be used for protein gels film or other samples that require trans illuminating white light This tray should be placed on the UVT and the 365nm wavelength should be selected while imaging
4. ES EE EE EE B B B B BG E E GN Software Features l N e e Select the tray type to be used e Select UVT for all flourescent gel applications e Select Chemi Tray for all chemi applications e Select White Light Conversion for all visible gel applications e Place the Flat Field Target in the system The target and tray used may need to be different than those selected in the software PLEASE CHECK CAREFULLY e f the UVT is selected place a full 8 2 x 11 sheet on the tray so that it completely covers the UV glass e fthe Chemi Tray is selected place a 3 x 3 section of paper in the filter wheel shield such that it covers the hole Please see Adding and removing filters in Appendix D for a visual aid e fthe White Light Conversion Tray is selected no sheet of paper is necessary e Close the door and select Build The system will automatically save the file to the correct location e Repeat these steps for each imaging environment desired The files will be applied automatically when the imaging parameters meet those selected in the Flat Field Correction generation Omega Lum Flat Field Trays and Targets The target used to construct the Flat Field Calibration reference image varies slightly depending on the application Generally a large 11 x14 target cut to cover the entire UVT is used Alternatively the white light conversion tray may be used in some cases When using the UVT as a light source the wavelength mus
5. Green Epi Blue SYBR Safe Epi Blue Coomassie Blue UV Light Visible Light Gels UV Light Chemiluminescent Blots None High Resolution Chemi Blots BUDE White Light Conversion Screen White Light Conversion Screen Chemi Tray Chemi Tray Elevated Fluorescent Westerns Epi Red Green Blue Chemi Tray Elevated The White Light Conversion Screen should be used with the 365nm UV wavelength For Omega Lum C and W Only For Omega Lum W Only Capturing Your Image Imaging with Default Applications Once you have determined the correct application for your desired excitation source place your gel or blot on the appropriate tray and log into the software e Select your Application from the Application menu Use the scroll bar on the right to move through the entire list e Select Auto or Manual exposure www gelcompany com EtBr UV 302nm SYBR Safe SYBR Safe Coomassie UV 365nm Coomassie UV 365nm Chemi Chemi Faint Bands Chemi High Res Fluorescent Westerns EtBr UV 302nm EtBr UV 302nm ImagingStudio Gallery Welcome Administrator EtBr UV 302nm e Select Capture e To stop capture for any reason simply select the Capture button again It now reads Stop e Your image will appear in the Gallery Tab Here you can make visual adjustments to the image and save or send the image to the desired location Manual Exposure Options Resolution and Binning Binni
6. Notifications Search Share General Privacy Devices Wireless Ease of Access Lock screen apps Sync your settings Choose apps to run in the background and sho e Scroll down the list under Advanced Startup select Restart Now a aa PC settings d ni The system will restart sr nnn Personalize Users Refresh your PC without affed If your PC isn t running well you can refresh it with Notifications videos nd Oia personal ler Get started Search Share Remove everything and reins General It you want to recycle your PC or start over compie settings Privacy GR van Devices Advanced startup Wireless Start up from a device or disc such as a USB drive l restart your PC Ease of Access Sync your settings C j e Select Troubleshoot Ch r CONS 2 8 Op lon Continue gt Exit and continue to Windows 8 turn orr your PC b e Select Advanced Options 9 Troubleshoot Advanced options www gelcompany com Detailed driver installation instructions are available in the Mitsubishi Electric P 95D Printer Installation Materials Select Startup Settings Select Restart Use the USB keyboard to select number hit F7 or the number 7 key The system will restart You are now ready to install the printer driver To start plug in the printer USB Turn on the printer and follow the directions that appear on the Screen Or you may install the printer from the contro
7. 365 nm Trans lllumination 25mm Motorized 21 0cm x 27 0 cm Maximum Field of View 16 bit Tiff Jpeg 110V 240V 33 0 cm x 48 0 cm x 74 0 cm 31 2 kg 8 4M pixels 25mm Motorized 20 0cm x 15 0 cm 110V 240V www gelcompany com System Setup Warning Excessive Weight Hazard Please use two or more people to lift the system Failure to do so may result in personal injury and or system damage As with all electrical instruments the Omega Lum imaging system should be located away from water solvents or corrosive materials Place the unit on a flat stable surface with adequate clearance and at least 4 inches on all sides Further the system should be placed away from interfering electrical signals and magnetic fields Gel Company recommends using a surge protecting power strip to prevent electrical damage to the system Remove the system from the packaging and place on the work surface The accessories are packaged separately in a smaller box Remove the accessories packaging and protective films e Plug in the Power Cords The Omega Lum has two power cables one powers the system components and one powers the onboard tablet Locate the power cables and plug them into the back of the system e Turn on the System Power The power switch is located on the lower right side of the cabinet It will take the system approximately 20 30 seconds to initialize once the power has been turned on
8. A EN61000 4 2 EN61000 4 3 EN61000 4 4 EN61000 4 5 EN61000 4 6 EN61000 4 11 UV Safety Precautions Because U V radiation can cause serious damage CA U TI 0 N to unprotected eyes and skin we recommend to wear U V protection glasses or face shield The Omega Lum systems come with a built in Ultra Violet UV 200 400nm Trans illuminator inside the system Exposure to UV radiation can cause permanent and or serious damage to the eyes and skin The system enclosure confines the radiation within the system and shields the user from exposure The system is also equipped with a two way safety interlock switch which automatically cuts off the power to the trans illuminator when the door is open during normal use The Omega Lum imaging system belong to Class A equipment and fulfills the limit values of table 3 but not table 4 of EN 61326 1997 A1 A2 A3 It may become necessary to override the safety switch or operate the transilluminator outside the system for service In this case be sure to use the following safety precautions e Always wear UV protected eyewear that is specified by the manufacturer as providing protection at the wavelength s used making sure that the eyewear protects any areas wear radiation may come through UV sunglasses may not prevent UV radiation from coming in through the sides or around the lenses e Always cover all skin that may be exposed to UV light especially the face neck hands and arms e Always mak
9. Software will be error free or will run without interruption THE FOREGOING WARRANTY IS EXCLUSIVE AND IN LIEU OF ALL OTHER WARRANTIES WHETHER EXPRESS IMPLIED OR STATUTORY REGARDING OR RELATED TO THE SOFTWARE OR ANY MATERIALS OR SERVICES PROVIDED TO YOU UNDER THIS AGREEMENT WE SPECIFICALLY DISCLAIM ALL IMPLIED WARRANTIES OF MERCHANTABILITY NONINFRINGEMENT AND FITNESS FOR A PARTICULAR PURPOSE www gelcompany com N i Appendix A 4 Limitation of Liability IN NO EVENT WILL WE BE RESPONSIBLE OR LIABLE TO YOU OR ANY THIRD PARTY FOR ANY LOST PROFITS OR INCIDENTAL CONSEQUENTIAL INDIRECT SPECIAL OR CONTINGENT DAMAGES ARISING FROM OR RELATING TO THE SOFTWARE OR THIS AGREEMENT EVEN IF WE HAVE BEEN ADVISED OF THE POSSIBILITY OF SUCH DAMAGES OUR TOTAL CUMULATIVE LIABILITY IN CONNECTION WITH THIS SOFTWARE LICENSE INCLUDING FOR ANY LIABILITY ON ACCOUNT OF A CLAIMED DEFECT IN ANY SOFTWARE PRODUCT WHETHER IN CONTRACT OR TORT OR OTHERWISE SHALL IN NO EVENT EXCEED THE PURCHASE PRICE OF THE SOFTWARE PRODUCT ALL SALES ARE FINAL NO RETURNS WILL BE ACCEPTED OTHER THAN AS PROVIDED IN THESE WARRANTY PROVISIONS 5 Interpretation This Agreement shall be governed by and construed in accordance with the laws of the State of California U S A without reference to its conflicts of law provisions Any dispute shall be subject to the exclusive jurisdiction of the state and federal courts located in Alameda County California U S A and you hereby irrevoca
10. Welcome Guest User Application Fluorescent Western Red Green Blue Saturation The Omega Lum Image Capture Software has the option to create custom applications allowing the user to tailor the imaging parameters While the parameters can always be modified prior to capturing your image creating a custom application saves these changes To create a custom application e Select the default application you wish to modify e Select Custom from the Application menu Omega Lum G You can customize your Light Source and Emission Filter Omega Lum C You can customize your Light Source Emission Filter Tray Type and Aperture Type Omega Lum W You can customize your Light Source Emission Filter Tray Type and Aperture Type General users can create and mange their own custom applications Custom applications created by the administrator will be available to all users but can only be modified by the administrator www gelcompany com ImagingStudio Gallery Settings Welcome Guest User Application ImagingStudio Gallery Settings Welcome Administrator Application Custom m Auto a t Light Source Blue LED Emission Filter Light Source Blue LED M Orange 590 50 M Gel Tray Aperture Type Gel Application Name required SYBR Safe 1 ka Creating Custom Applications Binning and Image Correction in Custom Applications Example on the Omega Lum C to create a custom UV g
11. mail Settings Display Name Chemi Orange 590 50 Filter 3 empty Filter 4 empty Filter 5 empty Filter 6 empty Display Name ImagingStudio Gallery Settings Welcome Administrator User Management Filter wheel diagnostics Status Filter wheel diagnostics in progress Cancel Flat Field Correction Build Flat Field Delete Flat Field Image Residual Bulk Image m Wheel Edit Emission Filters Emission Filter Diagnostics ImagingStudio Gallery Settings Welcome Administrator User Management Te Filter wheel diagnostics sme Change settings Darkmasters Status Filter wheel diagnostics completed PASSED Start Flat Field Correction Build Flat Field Delete Flat Field Image Residual Bulk Image H Wheel ission Filters Emission Filter Diagnostics L 1 Software Features Omega Lum C amp W Focus Calibration Filter Wheel Home Edit Emission Filters Not applicable to the Omega Lum G The Omega Lum C motoried lens focuses automatically and the calibration is set at the time of manufacture Over time and through use it is possible for the calibration of this focus to change In the event that the images no longer E mail Settings appear to be in focus the Focus Calibration Utility can be run The process is automatic and can take up to twenty minutes to complete Failure of the utility to
12. manuals for both the image capture and analysis software and the quick installation guide Gel Company recommends that the USB software drive be stored in a safe place for reference Starting the Software Launch the Software At the home screen select the Omega Lum tile The software will launch and you are now ready to use the Omega Lum system The capture software will launch after the Omega Lum tile is selected from the home window If it does not launch or if the software has been closed to the desktop it can be launched by double clicking on the desktop icon It can also be run from the program files folder Software License The first time you run the software you will be asked to accept the terms and conditions of the Omega Lum Image Capture Software License Agreement The complete software license is included in Appendix A To accept the terms and conditions select the Agree button To disable the software license popup check the box at the bottom of the window and then select Agree www gelcompany com Omega Lum Capture Software License Agreement The terms Aplegen us we and our refers to Aplegen Inc a California corporation Our contact details are listed on our website at www aplegen com contact php The term you refers to the purchaser or user of the Omega Lum Capture software Software As provided by the terms and conditions of sale incorporated into the quote and other sales documentatio
13. terms and conditions quote sales documentation and this Agreement the term sale or sold in connection with the Software means sale of a license to use the Software 1 License Grant Subject to the terms and conditions of this Agreement Gel Company grants you a non exclusive nontransferable license without the right to sublicense to run the Software on the Omega Lum instrument onto which it was pre loaded at time of purchase We reserve all other rights 2 Restrictions You acknowledge the Software constitutes valuable intellectual property protected by copyright trade secret and or other laws Except as expressly permitted in Section 1 above you will not a f Copy perform display use reproduce modify translate enhance decompile disassemble reverse engineer or create derivative works of the Software Rent lease or sublicense the Software Use the Software on a service bureau or application service provider basis Provide divulge disclose or make available to or permit the use of the Software by any third party Alter or remove any copyright notice or other proprietary rights notices that may appear on any part of the Software Export the Software without first obtaining any licenses that may be required under any applicable export laws 3 Warranty We warrant to you that the Software will perform substantially as advertised under normal use for a period of one year from purchase We do not warrant that the
14. the software and switch off automatically with image capture Epi LED lighting The cabinet contains epi white and blue LED panels for multiple imaging options The Omega Lum W also includes additional LED lighting for Multiplex analysis Tablet PC A touch screen PC for system control and image capture X Tablet Power Switch Tablet PC UV Indicator Light EE UV Safety Override Switch P sem R Epi LED panel UV Transilluminator 3 USB Ports UV Wavelength Selector www gelcompany com SA Omega Lum System Specifications Camera resolution Epi illumination Lens Image output Dimensions W x D x H Weight Camera resolution Epi illumination Lens Image output Power requirement Dimensions W x D x H Weight Omega Lum W System Specifications Camera resolution Blue Green Red amp White LED 302 nm 365 nm 457 nm 530 nm 682 nm Epi illumination Trans lllumination Lens Maximum Field of View 16 bit Tiff Jpeg Image output Power requirement 32 0 cm x 46 0 cm x 61 0 cm Dimensions W x D x H 36 3 kg Weight Omega Lum G System Specifications 6M pixels White amp Blue LED 302 nm 4 365 nm 18mm Fixed 21 0 cm xX 270cm 16 bit Tiff Jpeg 1 10V 240V Trans Illumination Maximum Field of View Power requirement 33 0 cm x 48 0 cm x 74 0 cm 31 2 kg Omega Lum C System Specifications 8M pixels White amp Blue LED 302 nm
15. ACT STRICT LIABILITY OR TORT INCLUDING NEGLIGENCE OR OTHERWISE ARISING IN ANY WAY OUT OF THE USE OF THIS SOFTWARE EVEN IF ADVISED OF THE POSSIBILITY OF SUCH DAMAGE 7 The Software uses the Freelmage open source image library See http freeimage sourceforge net for details Freelmage Is used under the Freelmage Public License FIPL Version 1 0 You may obtain a copy of the FIPL at http freeimage sourceforge net freeimage license txt Software distributed under the FIPL is distributed on an AS IS basis WITHOUT WARRANTY OF ANY KIND either express or implied See the FIPL for the specific language governing rights and limitations under the FIPL Aplegen Inc Omega Lum Image Capture Software License Version 1 0 May 7 2013 Contact Information Gel Company 655 3rd St Suite 240 Phone 415 247 8760 Tech Support 415 247 8760 san Francisco CA 94107 Fax 415 247 8765 Tech Support Fax 415 247 8765 USA Email gelinfo gelcompany com Email support gelcompany com NO o www gelcompany com Appendix B Safety CE CSA Conformity The following Omega Lum Imaging System Models Omega Lum G Omega Lum C Are in conformity with the provisions of the following EC Directives including all amendments and national legislation implementing these directive Low Voltage Directive 2006 95 EC EMC Directive 2004 108 EC And that the following harmonized standards have been applied EN61010 1 2001 EN61326 1 2006 Class
16. age Width Image Height Bit Depth Exposure Time ms Date Taken Taken By Comment Invert Inverts the black and while values of the image in the display Auto Contrast Calculates a balanced contrast ratio Zoom in Zooms in on the image Zoom out Zooms out on the image www gelcompany com T Software Features Settings Tab All users are granted access to the settings tab to use the following utilities the RBI utility filter wheel homing and e mail settings General users can also delete custom applications that they have created Additional Settings are restricted to the Administrator Residual Bulk Image Utility To ensure an optimal imaging environment during chem imaging the Omega Lum Image Capture Software offers the Residual Bulk Image RBI Utility The feature ensures the highest sensitivity imaging for chemi by removing trace residual photons from the camera sensor The RBI utility is ideal for situations where a bright UV gel blue light gel or white light gel image has been taken O 10 minutes before a chemi image needs to be taken e Select the Settings Tab Settings admin Administrator Reset password Generate Darkmasters e Select the Residual Bulk Image Utility It will take two 2 minutes to run Residual Bull Image posa Wheel Home e Once the utility has completed select the Imaging Studio Tab and Imagingstudia continue with image capture Welcomes Julien User
17. ations there are two additional exposure options in addition to the Auto and Manual Exposure Each exposure options has Its benefits e Auto Ideal for bright light images such as gel images using visible or fluorescent dyes Auto expose can be used for chemi applications However they are best used for very bright westerns whose substrate Is very stable and long acting gt 12hrs e Manual Ideal for know exposure times For example the exposure is always going to be 2 minutes Manual Exposure takes a single image at the time input e Multiple Multiple allows you to capture multiple images with difference exposure time www gelcompany com Edit Exposure Settings 1 minutes 0 seconds 0 milliseconds ImagingStudio Gallery Settings Welcome Administrator Application Chemi M Auto Live Marker O Saturation ImagingStudio Gallery Settings Welcome Administrator Application Chemi Exposure Manual M lt lt Marker Saturation AN ImagingStudio Gallery Settings Welcome Guest User Application Chemi Y Exposure Multiple M Total Images 1 Saturation RN e Example you could take three frames the first 10 seconds e The second 1 minute and the third 3 minutes Each frame is an individual image e Cumulative Cumulative allows stacking of multiple frames of ne Settings the same exposure time This option is deals for samples with Welcome Guest User an unknown expos
18. bianagement _ LLLLLLLLLLLLLLEE EE LT LINE EEE EE 8 www gelcompany com Software Features Filter Wheel Home The filter wheel of the Omega Lum homes automatically on startup If you F are uncertain of the filter wheel s position use the Filter Wheel Home Utility Filter Wheel Fa k to return to the home position p Settings _ Change E mail Settings E mail Settings The Omega Lum Image Capture Software can export your image via e mail To enable this feature you must first enter the e mail settings You will need your STMP service information Please consult with your system administrator of Change E mail Settings IT department as neededd E mail Settinas e To edit e mail settings select Change E mail Settings Poo E mail Settin FI In the requested information gt User Information E mail Address From Server Information SMTP Server Outgoing server SMTP Port Number My outgoing server SMTP requires authentication SMTP Authentication User Name Password Remove Custom Applications General users can use this feature to delete custom applications they created The view will only show those applications they created ay Ko www gelcompany com 203 Software Features Administrator Settings The Administrator can create and manage user accounts generate new bias and darkmaster files create flat field corr
19. bly agree to submit to the personal and exclusive jurisdiction and venue of such courts The various section headings used herein are inserted for convenience of reference only and shall not affect the meaning or interpretation of these Terms and Conditions or any section thereof If any term provision covenant or condition of these Terms and Conditions is held by a court or arbitral panel of competent jurisdiction to be invalid void or unenforceable the remainder of the provisions hereof shall remain in full force and effect and Shall in no way be affected impaired or invalidated 6 The Software incorporates software called AvalonDock copyright Xceed Software Inc pursuant to the terms of the New BSD License Additional terms conditions restrictions and warranty limitations regarding use of AvalonDock software may be found at http avalondock codeplex com AVALONDOCK SOFTWARE IS PROVIDED BY THE COPYRIGHT HOLDERS AND CONTRIBUTORS AS IS AND ANY EXPRESS OR IMPLIED WARRANTIES INCLUDING BUT NOT LIMITED TO THE IMPLIED WARRANTIES OF MERCHANTABILITY AND FITNESS FOR A PARTICULAR PURPOSE ARE DISCLAIMED IN NO EVENT SHALL THE COPYRIGHT HOLDER OR CONTRIBUTORS BE LIABLE FOR ANY DIRECT INDIRECT INCIDENTAL SPECIAL EXEMPLARY OR CONSEQUENTIAL DAMAGES INCLUDING BUT NOT LIMITED TO PROCUREMENT OF SUBSTITUTE GOODS OR SERVICES LOSS OF USE DATA OR PROFITS OR BUSINESS INTERRUPTION HOWEVER CAUSED AND ON ANY THEORY OF LIABILITY WHETHER IN CONTR
20. complete the calibration or if images continue to appear out of Custom Application focus after the calibration may be an Indication of a problem with the lens or camera please contact Gel Company at support gelcompany com Emission Filter Diagnostics Focus Calibration e Torun the utility select the Settings Tab e Select Calibrate Fmmssonrmer Chemi M e Select the Emission Filter Tray Type and Aperture Type of the application you are trying to focus emm a e Select Calibrate Aperture Type Chemi 0 95 M Calibrate Focus is affected by the choice of emission filter aperture setting and tray position There are many possible combinations of these three settings 48 so a calibration method for all of these combinations is provided in the SETTINGS tab After a calibration is complete capture an image to confirm accurate focus If the image Is not in focus switch to a target with greater contrast A business card is a good target for focusing If the calibration fails again it may be necessary to manually focus the lens In this case please contact Gel Company at support gelcompany com Commonly used configurations are calibrated at the factory and include the following Filter Tray Type Aperture Typical Application 1 Orange UVT Gel 2 8 EtBr UV 302nm 2 Orange Chemi Gel 2 8 SYBR Safe 3 Orange White Gel 2 8 Coomassie UV 365nm 4 Chemi Chemi Chemi 0 95 Wide Angle Chemi 5 Chemi Ele
21. d admin Full name Administrator Password Confirm password Email Profile image path C Program Files x86 Aplegen Omega L Captured image path Default path www gelcompany com Bias and Darkmaster Files Bias and darkmaster files provide for automatic background correction during long exposures They are unique for each camera Bias and darkmaster files have already been created for your system for 1 3 and 9 minute exposure If you anticipate taking longer exposures we recommend you create a new set of darkmaster files times To create darkmaster files login as Administrator and navigate to the Settings tab Select Generate Darkmasters Enter the desired exposure times in the popup window The exposure times would be comma separated with no spaces You must enter all the times you want such as 1 3 9 20 Click Generate The total time remaining for the completion of the darkmasters will show on the screen The new set of bias and darkmaster files will overwrite any previously generated files Flat Field Correction Darkmasters C Generate Darkmasters Residual Bulk Image Enter exposure time for each set of comma separated list of exposure ti Total Time DD HH MPM S5 Completion Time Flat Field Corrections are digital image adjustments that correct for artifacts in the optical path and pixel variations to improve image uniformity Flat field files are created at the
22. e Imaging Studio is your workspace for selecting and editing the image capture settings The Omega Lum has eliminated the need for focusing or aperture settings simply place your sample in appropriate gel tray select the application and click acquire Basic imaging involves 1 Positioning your sample 2 Selecting your application 3 Capturing your image Positioning Your Sample Place your gel either directly on the UV transilluminator or on one of the optional sample trays available from Gel Company Use the positioning markers as a guide The Omega Lum systems capture a large area so you can place your sample on any part of the imaging surface For ease of use Gel Company recommends that you place your sample centrally towards the front of the transilluminator In the Omega Lum C and W the chemi tray may be elevated In the elevated tray Gel Company recommends you place your sample in the center of the tray www gelcompany com I j MNN EERE REET Selecting Your Application The Omega Lum has a dual wavelength transilluminator epi white lights epi blue lights as well as a white light conversion screen and chemi tray Use the table below to select the correct combination for your application If you do not see your application listed please check Creating Custom Applications on page 15 Conversion Tray Omega Lum Application Sample Dye Excitation Ethidium Bromide UV Light SYBR
23. e sure that any UV protection devices such as the safety switch on the light cabinet apparatus are working properly If not discontinue use until the device s are properly repaired e Please use only UV lamps in the trans illuminator N p www gelcompany com N TELL Appendix B Safety Electrical Safety Precautions Be sure to take proper precautions when handling any electrical equipment NEVER work on any live circuit fixture receptacle or switch Safety rules you should follow whenever working with any electrical appliance include Always shut off power at the main disconnect before changing a fuse Always shut off power to the circuit before repairing or replacing a switch receptacle or fixture Always tape over the main switch empty fuse socket or circuit breaker you are working on Always check that the circuit is dead before beginning work on it Using a circuit tester or voltmeter can help you determine this Always unplug any appliance before repairing it The earth terminal intended for connection to an external protective conductor for protection against electric shock in case of a fault is located on the inside of the back panel Hot Surface Warning Under normal conditions the temperature of glass surface of UV transilluminator is below 50 C and safe to touch However if the system malfunctions it is possible for the glass surface temperature to exceed 80 C Please exercise caution whe
24. ection files edit emission filters and run diagnostics as well as change e mail settings and calibrate the focus The default password for this user is admin It is recommended that the administrator change the password once the system is set up and keep the new password in a safe location U se r P rofi e S ImagingStudio Gallery Settings Welcome Administrator User Management admin Full Name Administrator The Omega Lum Image Capture Software allows you to create individual user Reset password profiles User Name guest Darkmasters Full Name Guest User Delete Reset password e o create a new user profile login as the Administrator you will be redirected to Administrator Settings tab Flat Field Correction Build Flat Field iel Delete Flat Field Image Residual Bulk Image e Filter Wheel j e Select Create New Account and enter the requested information User Management Manage Existing Accounts Create a New Account Change settings e f no password is entered then no password will be required to sername Required login Full name Password Confirm password Email Profile image path B le Captured image path Default path Create e To change the admin password and settings select Change Settings User Management Manage Existing Accounts Create a New Account ty C Change settings Username Require
25. el application ImagingStudio Gallery Settings using the Blue LED and Orange Filter Welcome Guest Use Application Exposure e Select EtBr UV 302 e Se ect Custom 3 ImagingStudio Gallery Settings Welcome Administrato Application Custom Li MM Light Source Blue LED Emission Filte e Select Blue LED under Light Source Orange Filter under Emission Filter Gel Tray under Tray Type and Gel under a a Aperture Type Name the application and select Save Emission Emission Filter 590 50 The Custom Application option builds the application based on the original applications parameters which have defined sensitivity levels darkmaster correction and auto exposure methods e Gel Applications use standard sensitivity simplified bias and darkmaster corrections and normal autoexposure methods e Chemi Applications use enhanced sensitivity full bias and darkmaster corrections and normal auto expose methods e Chemi Faint Band Applications use high sensitivity full bias and darkmaster corrections and enhanced autoexpose methods www gelcompany com ESSE EE EE EB B B B GG G HH E GG GG GL En v The sensitivity mode is based on the original application while bias file and darkmaster correction as well as the auto expose method are determined by the final application name To use full bias file and darkmaster correction ensure that Chemi is included in the applica
26. l panel To reach the control panel open the Charms bar select Control Panel www gelcompany com Appendix C Tablet and Peripherals Automatic Repair Fix problems that keep Windows from Startup Settings Restart to change Windows options such as Startup Settings Press a number to choose from the options below Settings Se he Appendix D Routine Maintenance _ LLLLLLLLL DLL LEE EEE TIENNE EEE EE The Omega Lum System is robust and requires minimal routine maintenance The system should be kept clean and any spills cleaned up immediately Simple green or ethanol can be used to remove dried spills Use only a soft cloth to clean the UV transilluminator glass as harsh clothes can leave scratches Replace UV bulbs and the rubber camera gasket as needed If condensation or frost appears in an image it may be necessary to replace the desiccant plug in the camera Adding or Replacing Filters To add or replace a filter it must be physically added to the system as well as loaded into the software Position 1 should be left empty for chemiluminescent applications To start login as Administrator e To add a filter x e Create a custom application and select the filter position you am wish to add Nu uL 3 empty e Select Capture This will rotate the filter wheel into the desired RER 1 location Zoom In e Open the filter wheel shield e Screw in the new filter
27. min he Omega Lum C Omega Lum G EUM 1 1 2 yo m Omega Lum W aplegen The OMEGA LUM Family Imaging Systems User Manual px gA company Table of Contents Section Page Section Page System Overview 3 settings Tab 18 Residual Bulk Image Utility 18 Omega Lum System Specifications 4 iter Wiel iome 19 Omega Lum G System Specifications 4 E mail Settings 19 Omega Lum C System Specifications 4 Remove Custom Applications 19 Omega Lum W System Specifications 4 Administrator Settings 20 System Setup 5 aes Profiles 20 Adjust Tilt of the Tablet PC 6 DIAS SEE a UV Transilluminator and Pull Out Tray 6 pide PICICSCSMECEUN Omega Lum Flat Field Trays Additional Semis Trays 6 and Targets 25 Er Pale TON i Edit Emission Filters 23 A Sens a Emission Filter Diagnostics 23 Software 8 Omega Lum C amp W Focus Calibration 23 E 8 Appendix A 25 raning He SERNA Copyright and Trademark Information 29 pol Male CISRSE G Omega Lum Image Capture Software Logging 9 i ogging i License Agreement 25 The Imaging Studio 9 Contact Information 26 Positioning Your Sample 9 AA n Appendix B Safety 27 electing Your ication dd CE CSA Conformity 27 Capturing Your Image 10 al mQ UV Safety Precautions 2 maging wi efau ications d P f si 4 Electrical Safety Precautions 28 DE anual Exposure lions j Hot Surface Warning 28 H Exposure Options for Chemi 313 Ap
28. n you agree to be bound by the terms of this software license agreement Agreement You To start using the software you must agree to the terms of the software license agreement Press Agree to continue using the software press Disagree to exit the application ll Do not show this software license agreement again Agree Disagree of the FIPL at http freeimage sourceforge net freeimage license txt Software distributed under the FIPL is distributed on an AS IS basis WITHOUT WARRANTY OF ANY KIND either express or implied See the FIPL for the specific language governing rights and limitations under the FIPL Aplegen Inc Omega Lum Capture Software License Version 1 0 May 7th 2013 To start using the software you must agree to the terms of the software license agreement Press Agree to continue using the software press Disagree to exit the HR Il Do not show this software license agreement again Disagree Software Logging In The software starts at the User Login Screen e Select a user enter the password and click the Login button e The default Admin password is admin e There is no default password for the Guest user Leave the password box blank and click Login e The default users are Admin and Guest The Administrator can create additional individual logins Please see Creating User Profiles on page 20 The Imaging Studio Taking an image begins in the Imaging Studio Tab Th
29. n working around the glass surface in this instance Waste Electrical and Electronic Equipment WEEE ENG FRA GER ITA SPA SWE Lo 0 50 Eod Lx This symbol indicates that the waste of electrical and electronic equipment must not be disposed as unsorted municipal waste and must be collected separately Please contact an authorized representative of the manufacturer for information concerning the decommissioning of your equipment Ce symbole indique que les d chets relatifs l quipement lectrique et lectronique ne doivent pas tre jet s comme les ordures m nag res non tri es et doivent tre collect s s par ment Contactez un repr sentant agr du fabricant pour obtenir des informations sur la mise au rebut de votre quipement Dieses Symbol kennzeichnet elektrische und elektronische Ger te die nicht mit dem gew hnlichen unsortierten Hausm ll entsorgt werden d rfen sondern separat behandelt werden m ssen Bitte nehmen Sie Kontakt mit einem autorisierten Beauftragten des Herstellers auf um Informationen hinsichtlich der Entsorgung Ihres Ger tes zu erhalten Questo simbolo indica che i rifiuti derivanti da apparecchiature elettriche ed elettroniche non devono essere smaltiti come rifiuti municipali indifferenziati e devono invece essere raccolti separatamente Per informazioni relative alle modalit di smantellamento delle apparecchiature fuori uso contattare un rappresentante autorizzato del fabbrican
30. ng is the combining of digital pixels when taking an image Binning pixels increases sensitivity allowing fainter bands to be captured However the trade off is lowered resolution The Omega Lum Image Capture software offers three sensitivity modes Chemi Faint Bands Chemi and Chemi Hi Res Chemi Faint Bands uses a high binning mode for the greatest sensitivity Chemi Hi Res is un binned for the highest resolution The total resolution of the image is displayed above the capture button Exposure Settings The Omega Lum offers two exposure options on most gel applications and four options on Chemi applications For gel applications the most common exposure option will be auto and is the default setting If you select the manual option the Manual Exposure options will appear e To make changes select Edit to open the Edit Exposure Settings Window www gelcompany com 646 x 1120 Capture ImagingStudio Gallery Welcome Administrator Application EtBr UV 302nm Edit Exposure Settings 0 minutes 0 seconds 120 milliseconds TU EE E E E cg cg ne ae _ SS as ed ENS IE el NENNEN Ss oo I SS eal _ a F Software e Highlight the box you wish to edit and type in the desired exposure time e When done select the enter arrow far right then select Capture Exposure Options for Chemi Applications With Chemi applic
31. ny Better Tools for Less 665 THIRD STREET SUITE 240 SAN FRANCISCO CA 94107 WWW gelcompany com toll free 800 256 8596 tel 415 247 8760 fax 415 247 8765
32. plications 12 Waste Electrical and Electronic Equipment WEEE 28 Exposure Settings for Multi Channel Em TES CE A is Appendix C Tablet and Peripherals 29 Ht ise oe ee ican a Basic Tablet Operations 29 reating Custom ications id Mitsubishi Electric P 95D Printer Drive Installation 29 EE Software Features 17 b Imaging Studio 17 Appendix D Routine Maintenance 32 Gallery 17 Adding or Replacing Filters 92 Replacing UV Bulbs 34 or Ti 1 E www gelcompany com System Overview Congratulations on purchasing your Omega Lum Imaging System by Gel Company The Omega Lum is a powerful tool for gel documentation and chemiluminescent imaging It generates publication quality 16 bit tiff images and comes completely assembled for quick installation The Omega Lum Image Capture Software has a clean user interface for image capture and viewing With the superior optical design the Omega Lum captures high quality images without the need for focus or aperture adjustment Images generated by the Omega Lum are ideal for downstream quantitative analysis The Omega Lum Systems include the following key components 1 Front 2 Rear Cabinet The cabinet is a light tight imaging station Camera Integrated with the cabinet it is a high resolution cooled scientific grade CCD camera UV Transilluminator The UV transilluminator offers both 302nm and 365nm transillumination The UV lights are controlled via
33. t be selected correctly or the resultant Flat Field image will not be correct If additional filters and custom applications are used the appropriate setting will need to match those used to define the custom application Emission Light Application Filter Source Aperture Setting Target and Tray Used White paper inside filter Chemi Chemi none None Chemi 0 95 Chemi Tray wheel cover with 11 x14 on UVT EthBr UV302 Orange UV302nm Gel F2 8 UVT vr NOT paler On SYBR Safe Orange Blue EPI LED Gel F2 8 Chemi Tray Chemi Tray Coomassie UV Orange UV 365nm Gel F2 8 White Light White Light Conversion 365 conversion tray Tray 1 1 x17 white paper on Epi White Orange EPI White LED Gel F2 8 UVT UVT www gelcompany com Software Features Edit Emission Filters e Use this feature to name emission filters that are added later e Enter the name of the filter on the desired line e For information on adding and removing filters please see Appendix D Emission Filter Diagnostics The Emission Filter Diagnostics are performed if there is a suspected filter wheel malfunction It tests the filter sensor and timing e Torun the diagnostic utility select Emission Filter Diagnostics e Select Start e When it is complete you will get a pass or fail notification If you receive a fail notification contact Gel Company at support gelcompany com www gelcompany com Filter Wheel f F mail Settings Change E
34. te Este s mbolo indica que el equipo el ctrico y electr nico no debe tirarse con los desechos dom sticos y debe tratarse por separado Contacte con el representante local del fabricante para obtener m s informaci n sobre la forma de desechar el equipo Denna symbol anger att elektriska och elektroniska utrustningar inte far avyttras som osorterat hush llsavfall och m ste samlas in separat Var god kontakta en auktoriserad tillverkarrepresentant f r information angaende avyttring av utrustningen www gelcompany com Appendix C Tablet and Peripherals Basic Tablet Operations The Omega Lum uses a touch screen tablet PC to run the imaging station For in depth directions please refer to the Dell Tablet User Manual However here are some basic navigation tips to help you get started e Use your finger as you would a mouse e One touch or tap to select e To Scroll tap once then drag the scroll bar as desired e Double tap an icon to launch an application e o right click touch and hold your finger to the screen A square will appear When it completes lift your finger and the options menu will appear e To exit the menu tap anywhere on the desktop e The keyboard does not automatically pop up when entering text into a text box To open the keyboard look for the keyboard icon and touch it to select When saving you will need to open the keyboard prior to selecting Save e The windows button on the center bottom of
35. the tablet can be used to open the Windows Main Menu Mitsubishi Electric P 95D Printer Driver Installation For a quick and easy installation Gel Company has pre installed the Mitsubishi Electric P 95D Thermal Printer Driver To use simply plug the USB printer cable into the USB port indicated by the red dot on the side rear of the system To re install the driver or to install a different driver that Is not Windows 8 compliant please follow the directions below Windows 8 Driver Signature Enforcement is a new feature of Windows 8 that has certain signature requirements not yet implemented in the Mitsubishi Electric P 95D Printer In order to install the printer driver you must disable the Driver Signature Enforcement on startup install the driver and then reboot the system The Driver Signature Enforcement will reset upon rebooting but once the driver has been installed it will remain You will need a USB keyboard to complete the driver installation To install the Mitsubishi Electric P 95D Printer Driver please take the following steps e Open the Charms Bar swipe with your finger from the far right side of the screen to the left e Select Settings Omega Lum G BE ho www gelcompany com N D Appendix C Tablet and Peripherals e Select Change PC Settings Setting e Select General Ge settings Personalize Users Lock screen Start screen Acci
36. time of manufacture and applied automatically If these files need to be recreated the Flat Field Correction Utility will quickly create the necessary files Events that would require a new flat field correction include replacement of the UV bulbs corruption of the original files or replacement of the camera and lens Flat Field Corrections are created for each imaging environment Alterations in the emission filter light source aperture setting and working distance ie height of tray used all affect the final outcome A flat field file must be created for each imaging environment To create a Flat Field Correction file Select Build Flat Field from the Settings Tab Select the desired emission filter position Select the Light Source Note if using Transilluminating White Light the light source will be UV as the white light conversion screen converts the UV illumination source The White Light Conversion screen should only be used with 365nm UV When building UV flats make sure to note the UV wavelength Select the Aperture Type For gel applications select Gel F2 8 for chemi applications select Chemi F0 95 www gelcompany com Please select the light source emission filter tray type and aperture type then press the Build button to build a flat field image Emission Filter Light Source None i Aperture Type Chemi 0 95 Tray Type Chemi tray PBuld Software Features
37. tion name To ensure that enhanced auto expose is used include Faint Bands in the application name Please see the examples below for Illustration They are demonstrated on the Omega Lum G Example 1 to create a custom application with standard sensitivity and full bias file and darkmaster correction e Select a gel application such as Coomassie e Select Custom e Adjust the lighting exposure and display options as desired e Name the application Chemi Example 1 and Save Example 2 to create a customer application with enhanced sensitivity and simplified bias file and darkmaster correction e Select Chemi ej e Select Custom F ni itam Aa e Adjust the lighting exposure and display options as desired I e Name the application Example 2 and Save Example 3 to create a custom application with enhanced sensitivity simplified bias file and darkmaster correction and enhanced auto expose options e Select Chemi e Select Custom e Adjust the lighting exposure and display options as desired e Name the application Faint Bands Example 3 and Save For the Omega Lum C you will need to also select the Tray Type and Aperture Type www gelcompany com Software Features Imaging Studio Q Q Live Mode A quick display of what is inside the cabinet using the epi white lights for illumination This is not a preview of the image Sa
38. turation Oversaturated pixels appear in red Invert Inverts the black and white values of the image in the display Auto Contrast Calculates a balanced contrast ratio Zoom in Zooms in on the image Zoom out Zooms out on the image Capture Use this button to start the image capture process The Gallery Tab in the Omega Lum Image Capture software allows modification of basic visual aspects such as contrast and saturation Previously captured images can also be opened and modified Eu lE DO s 000 e Open Open an image from a saved file location Print Print the currently selected image Save Opens the Save or Save As dialogue boxes The keyboard does not automatically pop up when entering text into a text box To open the keyboard look for the keyboard icon and touch it to select When saving you will need to open the keyboard prior to selecting Save Save All Allows the user to save multiple images at the same time Crop Opens the Crop Image tool A box will appear with blue handles on the image To crop adjust the handles to the desired size and select Crop Confirm The cropped image will display in a new tab Contrast Use the slide bars to adjust the image contrast E mail Use to send image files Marker Only available for use with chemi applications creates a new image that overlays the marker file and the chemi image Image Info Displayed Information Im
39. ure time and a substrate with a short half life Cumulative can capture multiple images before the signal decays SLT ECO Chemi Marker e Example you capture three frames at 10 seconds This first image will have an exposure time of 10 seconds the second image will have an exposure time of 20 seconds and the third image will have an exposure time of 30 seconds e Please note that stacked images have a higher background level because the background of these image Is also stacked m Co www gelcompany com I m l 4 Exposure Settings for Multi Channel Fluorescent Westerns The Omega Lum W offers multi channel fluorescent imaging for the Cye2 Cye3 and Cyeb channels This allows the detection of multiple proteins in the same blot as well as distinguishing between closely migrating bands without the need to strip and re probe To use this feature of the Omega Lum W select the Fluorescent Western Application The Fluorescent Western Application offers three channels and each channel has two exposure options Auto and Manual To select your exposure options first select the channel you wish to work with Red Green or Blue Then select Auto or Manual exposure If you select Manual exposure select edit to open the Edit Exposure Settings box to input your desired exposure time Repeat these steps for all three channels then select Capture Custom Applications ImagingStudio Gallery
40. vated Chemi 0 95 Chemi Hi Res 24 www gelcompany com Appendix A Copyright and Trademark Information All goods and services are sold subject to the terms and conditions of sale of the company within Gel Company which supplies them Gel Company reserves the right subject to any regulatory and contractual approval if required to make changes in specifications and features shown herein or discontinue the product described at any time without notice or obligation Contact a Gel Company representative for the most current information Omega Lum UltraQuant and the Aplegen workmark are a trademark of Gel Company All other trade names are the property of their respective owners Gel Company 665 3rd St Suite 240 san Fancisco CA 94107 USA Omega Lum Image Capture Software License Agreement The terms Gel Company us 77 44 we and our refers to Gel Company a California corporation Our contact details are listed on our website at www aplegen com contact php or www gelcompany com The term you refers to the purchaser or user of the Omega Lum Image Capture software Software As provided by the terms and conditions of sale incorporated into the quote and other sales documentation you agree to be bound by the terms of this software license agreement Agreement You acknowledge that all Software is merely licensed to you and that title to the Software or any copies thereof is not transferred to you As used in the
Download Pdf Manuals
Related Search