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        Leica DC Cameras with IA Applications
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1.   From the Windows Control Panel select the Leica Imaging application  the one that has the Leica logo   this  shows the Server select dialog     Select the required driver from the Image Server configure dialog   gt  Select Image Driver  Select Preview and see  that the DC Twain window appears      Now check OFF the DC Twain selection and check ON the Peripheral server and install it  Please use the DM  microscope SDK that is automatically installed from the Server CD  Make sure you select the correct radio button    for the model of DM Microscope you will be using  If you have a later version e g  V4 13  please completely  uninstall this  Also you must manually remove the remaining configuration files      Step 3  If all is OK so far you should now be able to install the IA software as normal     n   Refer to Read the Release notes on the CDs before you start the installation  A    Hints    If you have messages on starting the camera suggesting that the camera is not connected when it obviously is    try closing the application and rename the    LastUsed    configuration file from the folder WinNT Twain_32 LeicaDC   If these files are corrupt or not compatible the cameras will not start     DC300 resolution is high and can be used with Leica QWin  However you cannot see the whole image on the  screen at full resolution so the DC300F and DC350F are better choices to use with Leica QWin     With Leica QWin  when using high res cameras  go to preferences and check on all the f
2.  uninstall is performed  Also rename as    old    the configuration files in the Twain32 directory these are not  necessarily compatible with a different version of DC Twain     Install the DC Camera software first  carefully following the instructions provided on the CD  Make sure that this is  working correctly  e g  using Windows Imaging  PaintShop or IM 50  before installing Leica Servers     Leica DC Camera  with lA applications 1 Installation advice Issue 2  Feb 2002      Note  If the cameras are not working  do NOT proceed  You will only compound any problems  You must  have the DC Cameras working independently of the IA applications before you install the IA  applications  Check the camera installation as described on the DC camera CDand retry the installation     Step 2  Install Server CD  You will find the user manual for the server on the CD  Read it before proceeding     Note   Only install one server component at a time and check it is working correctly before you install another  component   You should only have one tick box set for installation at a time      Warning   the uninstall button will remove ALL servers not just those with ticks new to them  Use with extream  care     Install Leica DC Twain from the Server CD   Do not install the old drivers for DC cameras using PCI3 interface  boards  these boards are not compatible    Do not re install any other drivers from the Server CD at this time  for  example M eteor drivers  M ake sure all are checked OFF    
3. Leica DC Cameras with  IA Applications    Installation advice Issue 2    Leica DC Cameras with IA Applications  Installation Advice Issue 2    Abstract    All reasonable steps have been taken to ensure that this publication is correct and complete  but should any user  be in doubt about any detail  clarification may be sought from Leica Microsystems Imaging Solutions Ltd  or their  accredited representative  The information in this document is subject to change without notice and should not  be construed  as a commitment by Leica Microsystems Imaging Solutions Ltd  Leica Microsystems Imaging  Solutions Ltd accepts no responsibility for any errors that may appear in this document     rc       Leica Microsystems Imaging Solutions Ltd  England  2002    No part of this publication may be reproduced  transmitted  transcribed  stored in any retrieval system or  translated into any human or computer language by any means or in any form  without the prior written  permission of Leica Microsystems Imaging Solutions Ltd        Microsoft and MS DOS are registered trademarks and Windows is a trademark of the Microsoft Corporation     Date  Feb 2002  Issue  2  Printed in UK    Introduction    The following notes aim to give some guidance in the installation and use of the Leica IA applications IW  MW   CW and FW that have been updated to work with the new DC cameras     The compatible DC cameras are those that use the new PCI4 Hotlink board and the DC500 camera that uses the  Firewire boa
4. lags  fit to window etc    However this can slow down image refresh  so you have to decide which is the best compromise for the way you  are using the software     With Leica QWin  make the image memory suitably large e g  100M in the System Configuration dialog     Leica DC Camera  with IA applications 2 Installation advice Issue 2  Feb 2002    For use with IA apps  switch off the auto gamma and auto contrast in the DC Twain  Do a Reset of these values  If  you leave these auto functions on  the intensity of the image can vary  this is OK for image documentation but can  be a problem when you want to use a fixed detection threshold     For use with IA apps do not use Auto Exposure for repeated image grabbing   This function is in the Image Server  dialog   so it can be used to quickly get an image  however once you know the settings to use  Auto Exposure is  not recommended as it slows down the acquisition rate      For best colour rendition set the gamma to a value of 1 and only make small changes to gamma  You will find that  small changes to gamma can have a large effect on the image quality     Note  with the DC300F and DC350F there is a non linearity in the exposure time at around 114 5ms  due  to the physical properties of the camera  it is best to adjust the light level so that you are faster or  slower than this     To display live images with fastest refresh rate you should have an acquire time of less than 114 msec    Be aware that the resolution of the images disp
5. layed in live mode  for DC300  amp  DC500  is lower than the final  acquired image  For example you may see pixelation and aliasing  This is reasonable so that you have a fast  image refresh    When you use a DC500   Leica QFluoro with narrow land filters  the exposure can appear good on the screen live  image  but when you grab an image it is darker than expected  for red and blue filters  not green   At the moment  there is no explanation for this  However when you switch the camera to 16 bit mode you can use the Auto   scaling function to get high brightness images and this works well     In Leica MW when using interactive measurements  switch off    fit to window     this speeds up the image refresh   Limitations    A  Note  There are some image resolutions that do not acquire good images and these issues will be  resolved in a later release  These are DC500 3900 x 3090 9 shot and DC300 with the expanded resolution  image  For the DC500 using 2600 x 2060 16 shot  please switch graphic display to 24 bit colour    DC cameras cannot be used with Autofocus functions of the Peripheral Server as used by QWin and MW     Some of the Leica MW processing functions  e g  QGrain  are limited to 2k x 2k images due to the speed of  processing of large images     The resolution of the image selection with DC500 does not display correctly with DC Twain version 4 1 1     In general for IA applications  you will find that images of 1280 x 1100 or smaller give good performance and you  can 
6. rd  These notes are for use by qualified Leica personnel only     al Note  Additional information is in the release notes which are located on each of the application CDs   Please read these before you start to install  These notes are in pdf format     The user manuals for the Server CD are also included on the Server CD in paf format     CD s required for DC Camera use    The following CD versions are essential for compatibility with DC cameras  Earlier versions of the applications will  not work with the new camera drivers  DC cameras cannot be used with autofocus     DC Camera Version 4 1 1  This is an interim version for use with IA software only  Please do not use later  versions until further notice as there are known incompatibilities     E    eica Server V2 1 2    rc  D    ica QWin V2 5 1    Leica MW V3 2       Leica FW V1 2 1    Installation    WARNING  These are for Windows 2000 only with at least 256MB ram installed in the PC  For use with  A images greater than 2k x 2k 512M B ram should be installed     The simplest and easiest installation will be from a newly installed version of Windows 2000 that fas no previous  drivers or applications installed     After each stage of the installation re boot the PC   Step 1  Install DC Camera software    If you already have DC Camera software installed  check that the cameras are working  Completely uninstall any  previous version of the DC Camera software unless it is the version specified above  It is essential that this 
7. see the whole image on a monitor set to a suitably high resolution     These IA applications are for Windows 2000 only with at least 256MB ram installed in the PC  For use with images  greater than 2k x 2k  512M B ram should be installed  or 768 M B ram with Qfluoro      Leica DC Camera  with IA applications 3 Installation advice Issue 2  Feb 2002    Leica Microsystems Imaging Solutions Ltd  Telephone  44 1223 411101  gt   Clifton Road Fax   44 1223 412526 etca    Cambridge CB13QH imaging support leica  microsystems com MICROSYSTEMS  United Kingdom www microscopy imaging com    
    
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