JuLi User Manual
Contents
1. User Interface Product OVervieW Operation of the JuLI Bright field imaging Fluorescence imaging Merging Images Fluorescence and Bright field Time lapse imaging Transferring data to a PC Updating software Care amp Maintenance 22222222222200000000000e0e000s0eeeeeeeececssssee Troubleshooting 2 222 222 2 Warranty 22 22222222222 nese nenn en eneenneee Appendix 22 nnn n nen e nen n nnn n nnn e eee e ene neee n Technical Support 22 2 2222 222000222222 2ne 10 11 12 13 15 16 17 19 21 22 23 24 25 26 2 Product Contents JuLI is shipped with the following components Once you receive your instrument please check that all items listed below were2. pe beg aa RT ang ere eer ia 5 g ory Ah E gt 2 arg lt gt a wae F s KJ v rm 4 x 4 A 6 While viewing cells in the Zoom mode use the Focus knob to further adjust the image 7 Press the Capture button to acquire image 8 Turn on illumination using the GFP button located on the right side of the LCD touch screen 17 Operation Merged Image Fluorescence and Bright field continued 9 Adjust the illumination intensity if necessary using Exposure and Brightness buttons 10 Adjust the image by using Focus knob 12 Click the Capture button to acquire the image and then the Merge button will be activated 13 Press the Merge button to show the merged color image 14 Press the Save button to save the image The Save screen will pop up 18 Operation Time Lapse Imaging With JuLI you can set up your cells and program the JuLI to record time lapse images To use this feature click the Movie button program the settings number of Images period save the file name and click the Rec button 1 Press the Power button to start the JuLI at room temperature for approximately 20 minutes before placing into the incubator 2 Place the sample on the stage 3 Turn on illumination using the GFP or Bright button located on the right side of the LCD touch screen 4 Adjust the illumination intensity if necessary using the Exposure and Brightness button 6
3. Adjust the image by pressing the Zoom button and choose the section 7 While viewing cells in the Zoom In mode use the Focus knob to adjust the cell image 8 Click the Movie button to make the time lapse imaging 19 Operation Time Lapse Imaging continued Movie Option 0 Hour 10 Min 1 Min 10 Images Bright GFP UGLE y gt Apply 8 Press the Required Time button and enter the total running time It will be automatically displayed the total images 9 Press the Period button to set the time interval 10 Press the Save file name to save the movie name 11 To create a movie file choose the Bright GFP Merge button Bright button To record the bright movie only GFP button To record the GFP movie only Merge button To record the merged movie only Bright GFP To record the bright and GFP movie Bright GFP Merge To record the all channels If you use the GFP cover the only GFP images can be acquired 12 Press the Apply button to confirm the movie options 13 Press the Rec button to begin time lapse imaging JuLI will display the live cell images during the time lapse imaging 20 Operation Transferring Data to a computer 1 To archive your data or generate a printed report insert the JuLI SD card into the SD port 2 Save your data on the SD card by pressing the Save button on the main screen The image is automatically saved as a bmp file that can be opened with the JuLI imagin
4. for repair service contact sales digital bio com or your local distributor 25 Appendix The following products can be used with the JuLI Smart fluorescent cell analyzer and are available separately from Digital bio For more information visit www digital bio com Product Quantity Catalog no JuLI Smart fluorescent cell analyzer 1 JULI B0O04 JuLI SD card 1 Instruction Manual and PC software included JuLI Adapter with power cods 26
5. inlet SD slot 11 Description User Interface Preview Zoom Merge Save Image control JULI smart fluorescent cell analyzer Light source Power control Bright Field GFP Time lapse Movie Info Exposure Brightness The touch screen user interface of the JuLI Smart fluorescent cell analyzer provides new tools to expand cellular research through proprietary software Insightful data can be acquired such as proliferation assays and live cell counting Software Highlights v Live cell imaging Y Bright field Fluorescent cell images v Time lapse imaging v Merged bright field fluorescence images 12 Product overview Increasingly researchers are using live cell imaging to study cellular functions The JuLI a smart fluorescent cell analyzer was developed to easily view and capture images from live cell experiments The JuLI uses state of the art optics and image analysis to obtain fluorescent live cell images from cell culture dishes in a sterile hood The compact design allows installation in a tissue culture hood or incubator limits contamination The JuLI is able to capture sequential time lapse fluorescence and or bright images and which can be converted to movie files Growing live cell movies can be generated even in cell culture incubator The JuLI performs fluorescent bright cell counting measurements using the user friendly image processing software 13 Product o
6. shipped lf any items are missing or damaged contact your local distributor or sales digital bio com Items Quantity JuLI Smart fluorescent cell analyzer 1 Plate cover for GFP Adaptor 12V Power Cord 4 pcs set for U S Canada Taiwan Japan Europe or UK JuLI SD card Instruction Manual and PC software included Instruction Manual Quick reference card After receiving JuLI examine carefully for any damage incurred during transit All damage claims must be filed with the carrier CAUTION Neglecting to remove any or all shipping brackets or foams prior to operation may result in A damage to the equipment The shipping brackets or foam inserts must be reinstalled prior to shipping the unit to prevent damage to the equipment Safety Precautions Review and follow the safety instructions below f water or other material enters the instrument adaptor or power inlet disconnect the power cord and contact a service person For operating environment refer to Product Specifications next page Do not touch the main plug or power cord with wet hands Always ensure that the power supply input voltage matches the voltage available at your location This instrument is air cooled and its surfaces may become hot during operation When installing leave a space of more than 10 cm 4 inches around the instrument and do not place any objects between the instrument and the any walls Do not ins
7. NESMU JUL OO1E V 1 1 JULI Smart fluorescent cell analyzer User Guide www bulldog bio com phone 603 570 4248 fax 603 766 0524 4515 Culver Road Suite 307 Rochester NY 14622 Developed and Manufactured by NanoEntTek Inc NanoEntTek Inc HQ 12F Ace High end Tower 235 2 Guro 3 Dong Guro gu Seoul 152 711 Korea Tel 82 2 6220 7942 Fax 82 2 6220 7721 Copyright 2010 by NanoEnTek Inc All rights reserved Published in Korea All the materials in this user guide are protected by Korean and international copyright laws They cannot be reproduced translated published or distributed without the permission of the copyright owner JuLI User s Guide la Manufactured by NanoEntTek Inc 12F Ace High end Tower 235 2 Guro 3dong Guro u Seoul 152 711 Korea Tel 82 2 6220 7942 Fax 82 2 6220 7721 The information in this manual is described as accurately as possible Firmware and software changes and updates may change without prior consent or notification Copyright 2010 by NanoEnTek Inc All rights reserved Published in Korea Documentation NESMU JUL 001E Revision History V 0 0 Date Nov 2009 Revision History V 1 1 Date July 2010 Table of Contents Description of the JuLI Front VieW Rear VIEW
8. and Brightness buttons 5 The Zoom button and the section window allow the user to magnify the desired region using software 6 While viewing cells in the Zoom mode use the Focus knob to further adjust the image 7 Press the Capture button to acquire image 8 Press the Save button to save the image The Save screen will pop up 15 Operation Fluorescence imaging 1 Press the Power button to start the JuLI and display the main screen 2 Place the sample on the stage 3 Turn on illumination using the GFP button located on the right side of the LCD touch screen 4 Adjust the illumination intensity using the Exposure and Brightness buttons 5 The Zoom button and the section window allow the user to magnify the desired region using software 6 While viewing cells in the Zoom mode use the Focus knob to further adjust the image 7 Press the Capture button to acquire image 8 Press the Save button to save the image The Save screen will pop up 16 Operation Merged Image Fluorescence and Bright field 1 Press the Power button to start the JuLI and display the main screen 2 Place the sample on the stage 3 Turn on illumination using the Bright button located on the right side of the LCD touch screen 4 Adjust the illumination intensity using the Exposure and Brightness buttons 5 The Zoom button and the section window allow the user to magnify the desired region using software eee a 4 e
9. ediately If liquid spills on the JuLI turn off the power immediately and wipe dry The JuLI does not need regular maintenance To troubleshoot problems with JuLI contact Technical Support page 27 IMPORTANT Never disassemble or service the JuLI yourself Unauthorized repairs may damage the JuL I or alter its functionality which will void your warranty Contact sales digital bio com or your local JuLI distributor to arrange for service IMPORTANT Do not soak any surface in ethanol NEVER spray ethanol on the JuLI Wipe surfaces with ethanol soaked paper towels instead IMPORTANT Never expose JuL I to UV sterilization UV degrades many materials including plastic Damage from UV exposure is not covered under the manufacturer s warranty 23 Troubleshooting Poor merged image Poor bright field image Poor fluorescence image LCD screen is black Saving problems Merge button does not respond when clicked Bright field time lapse images become dark and bright JuLI does not power up Re capture images in bright field fluorescence Re optimize the brightness and exposure value Reset on the power button Re optimize the brightness and exposure value Confirm fluorescence with a positive control sample Touch the LCD screen with your finger Verify power supply is connected and power switch is on Click the Bright or GFP button Reset on the power button Do not sa
10. g program 3 Enter the file name using the keypad buttons displayed on the Save menu Save a ete Tine stamp LJ A2 S 4 5 0 7 8 19 0 ee QW EPRI UT Y UO P IPE NI SVD PIF O hi dpi pit Ke HAIX TC BV ED TING ly Space 4 Transfer the JuLI SD card to the SD port on your PC You may open the bmp file using the JuLI imaging program 21 Operation Updating software Periodically Digital Bio adds functionality and other improvements to the JuLI user interface We recommend keeping your JuLI up to date with the latest software If you have any questions about software updates contact sales digital bio com 1 Visit the JuLI website at www digital bio com to download updated firmware which is supplied as a zip file to your desktop 2 Unzip the zip file and save the firmware to the SD card supplied with the instrument 3 Insert the SD card into the SD port on JuLI Infomation 5 Click the Update button The update process takes a few minutes 6 When the update is complete you are prompted to restart the instrument by pressing Restart 22 Care amp Maintenance Clean the surface of the JuLI instrument with a damp cloth To clean the LCD screen turn off the JuLI instrument disconnect the power cable and clean the LCD screen with a soft cloth lightly moistened with LCD cleansing detergent Cleaning the screen with excessive force can damage the LCD the screen Wipe the screen dry imm
11. ice in hood or incubator prevents contamination by maintaining a sterile environment Also the easily viewed display allows quick and convenient to use in a cell culture incubator for capture time lapse imaging Dimensions amp Features Width Depth Height Weight Power Cord Cell Culture Hood Cell Culture Incubator IMPORTANT AN Do Not directly spray ethanol anywhere on the JuLI Always wipe surfaces with ethanol soaked paper towels instead Description Front view The LCD Touch Screen located at the front of the instrument contains buttons for necessary functions and displays bright field and fluorescence images The Focus Knob is used to adjust the image quality to obtain better bright and fluorescent cell images The Capture button is used to acquire bright field and fluorescent images s Neck Yus W Focus Knob Capture button LCD Touch Screen 10 Description Rear view The Power button is used to turn the instrument on and off The SD slot allows you to transfer and save images to your PC for data storage and printing purposes The SD card supplied with the instrument is inserted into the SD slot for data transfer See page 21 for Transferring Data to a PC The Power Inlet connects the JuLI to an electrical outlet using the supplied power cord and the appropriate plug based on the electrical outlet configuration in your country Rear view of JuLI Power button Power
12. itation 520nm emission Optional White Green LED RFP 530nm excitation 590nm emission CMOS 1 3M pixels 1280X1024 7 TFT LCD WVGA 800X480 lt 5kg 240X350X320 mm SD card 8G Setup 1 After unpacking the instrument place the instrument on a level dry surface Allow at least 10 cm 4 inches of free space at the back of the JuLI to allow for proper ventilation and prevent overheating of electronic components 2 Plug the supplied power cord into the JuLI instrument Attach the appropriate plug adaptor based on the electrical outlet configuration of your country 3 Plug the power cord into the electrical outlet Be sure to use only the power cord supplied with your instrument Powering the instrument with an unapproved power cord may damage the instrument 4 When ready to use start the JuLI instrument by pressing the Power button IMPORTANT NEVER grasp the neck of JuLI to transport Always carry the JuLI with both hands Place your hands and grasp the sides of the base avoiding LCD screen IMPORTANT NEVER expose JuL I to UV light UV light degrades many materials including plastic Damage from UV exposure is not covered under the manufacturer s warranty Setup Installing JuLI in a Cell Culture Hood or a Cell Culture Incubator Fluorescent live cell images from various cell culture dishes are directly captured in a cell culture hood This compact design allows installation of dev
13. tall the instrument on a slant or a place prone to vibrations which induces the risk of instrument malfunction or damage of the instrument Never insert any objects into the air vents of the instrument as this could result in electrical shock personal injury and equipment damage Plug the power cord firmly into the wall outlet and AC adapter To avoid potential shock hazard make sure that the power cord is properly grounded Be sure to position the equipment such that it is easy to disconnect Turn off the instrument before unplugging the power cord and or moving the instrument f the instrument is dropped or broken disconnect the power cord and contact a service person Disassembly of case will void warranty Use only authorized accessories adaptor power cord and SD card Symbols OCA O ooe Oo Protective earth Ground C This instrument and consumables conforms to the Declaration of Conformity Environmental Conditions Operating Power Frequency Electrical input Installation site Operating Temperature Maximum Relative Humidity Instrument Specifications CPU Magnification Filter Light source Camera Display Weight Size Data storage Product Specifications AC 100 240V 50 60Hz 12 VDC 1 0A Indoor use only 10 45 C 20 90 AMD AU1250 Objective 4X and digital zoom 10x and 20x compatible Excitation Emission Dichroic filter White Blue LED GFP 488nm exc
14. ve too many files on the SD card Click capture first It is only possible to merge an image that is captured as bright field and fluorescence images Check for and remove any condensation on the lid of the culture dish Check power switch on right side of unit Check power source or contact your distributor Warranty Digital Bio warrants that the JuLI will be free from defects in material and workmanship for a period of one 1 year from date of purchase If any defects occur in the JuLI during this warranty period Digital Bio will repair or replace the defective parts at its discretion without charge The following defects however are specifically excluded 1 Defects caused by improper operation 2 Repair or modification done by anyone other than Digital Bio or an authorized agent 3 Damage caused by substituting alternative parts 4 Use of fittings or spare parts supplied by anyone other than Digital Bio 5 Damage caused by accident or misuse 6 Damage caused by disaster 7 Corrosion caused by improper solvent or sample For your protection JuLI units being returned must be insured against possible damage or loss Digital Bio cannot be responsible for damage incurred during shipment of a defective instrument It is recommend that you save the original packing material in which the instrument was shipped This warranty is limited to the replacement of defective products For any inquiry or request
15. verview continued Important features of the JuLI smart fluorescent cell analyzer All in one stand alone systems The compact easy to use design allows the JuLI to be placed in a tissue culture hood or incubator Integrated display and on board computer allows JuLI to operate PC free 40 000 hr lifetime White Blue LED LED light utilized for bright field and fluorescence imaging has a life expectancy of approximately 40 000 hours Consistent performance over multiple years of use is provided with the white and blue LEDs Dark room free Unlike a conventional microscope fluorescence imaging can now occur with the room lights on and out in the main laboratory area Counting amp analyzing with image processing software Proprietary software expands the applicability of the JuLI Smart fluorescent cell analyzer Fluorescence experiments such as proliferation assays live cell counting or biomarker labeling Communication through wireless data transfer Utilizing Wi Fi technology all data including images and movies can be transferred automatically from JuLI to PC Upcoming technology 14 Operation Bright field imaging 1 Press the Power button to start the JuLI The main screen will be displayed 2 Place sample on the stage 3 Turn on illumination using the Bright button located on the right side of the LCD touch screen 4 Adjust the illumination intensity using the Exposure
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