Harvard Apparatus FlowControl Software
Contents
1. Leaving the execution task To close the Method Execution window press the Execute Methods button located in the experimentation assistant bar i Pressing the Execute Methods button to close the window implies LU a request to end the execution of all the methods You will be warned before exiting the execution task FlowControl User Manual 2011 Harvard Apparatus 48 5421 002 Rev B Appendices Connectivity Cables i Descriptior Catalog No P Pump to PC Cables choose 1 i Ea R5 232 9 pin d sub to 9 pin d sub 6ft 70 4004 Daisy Chain Cables add 1 per additional pump RS 485 1 5f 70 4000 jeje RS 485 6 ft 70 4001 HE Specialty if necessary USB Serial Adapter FlowControl User Manual 2011 Harvard Apparatus 49 5421 002 Rev B2. W Link to previous Step Loop properties Enable loop From To Iterations Finally the inserted step is automatically selected and the method is recalculated so that its overall information is updated within the status bar see page 27 Removing Steps From the Method Only the selected step may be removed by means of the Delete button of the toolbar or its equivalent option within the contextual menu of the selected step Before completing the removal task you will be warned and asked to confirm the operation Once confirmed the step is removed and the method is automatically redrawn to show its new steps sequence and overall information within the status bar see page 27 When a step is removed both the preceding and following steps keep the same duration and step linkage See page 30 However start and end flow rates are recalculated from the new steps sequence Defining a Loop Within a Method A Loop Feature is provided to allow the user to define a step or number of steps that will be repeated within a method To create a loop check the Enable Loop box located in the upper right corner of the Method Setup screen Press the button to open the Method Loop Properties screen Enter the steps that you want the loop to start and end with and the number of times the loop will be repeated then press OK Steps within the loop will be highlighted in orange and yellow Method Setup 123 Method name M
3. 34 Accepting and Cancelling a Method Edit rarnrnnnnnnennnnnnnnvnnnnnn 37 Copying a Methods 522242 37 Importing and Exporting Methods rrrvrnnvrernnrrvrrnvrenrnnernrrnvnenr 37 EXECUTING METHODS sirrien ia apana aiaa aei 40 Assigning Methods to a PUmp rrrrnnnrnnnnnnvnrnnrrrrnnnrenrnnrrennnnrennnn 41 Method Execution Control cccccccccceecceceeseeeseeeeesaeeeeeseeeeeeaees 42 FlowControl User Manual 2011 Harvard Apparatus 2 5421 002 Rev B Starting Pausing Resuming and Stopping Methods 42 Runtime Execution Information ccccccceceeeeseeeseeeeeeeeeeseeeneees 43 Controlling Special Situations During the Method Execution 44 Method Progress window ccccseceecssseeceeeeecseseeeceeseeeseueessaaes 44 Method Data Logging ccccsecccccsseeeceeeeeseeeeeseueeesseeeessneeeesaaees 46 ENC Clit Cl Olea usenet EE EEE EE EEE ER 46 Bitmap RODO as oe seende 48 xpernmenmal N ES aar ERT 48 Leaving the Execution Task ranunnnnnrnnnnrnnnnvnnnnrnnnnrennnrrnnnrrnnnerennn 48 APPENDICES Je 49 CONnNeCIIVITY Cables sagaer 49 FlowControl User Manual O 2011 Harvard Apparatus 3 5421 002 Rev B INSTALLING FLOWCONTROL Before using FlowControl check the system requirements and then install the software onto your hard drive System Requirements Although FlowControl is an easy to use software program the processing of the data requested and generated by the pumps mobi
4. Execute a Method Start method Monitor using Method Progress window Export Data Log N Flow chart outlining how to run FlowControl DEFINING PUMPS Open the FlowControl software by double clicking on the corresponding icon in the Windows desktop An initial blank screen will be opened FLOWCONTROL v1 0 00 Der File Configuration View Window Help T Configure Pumps bi Flow Control E HARVARD APPARAT US SOFT VVARE FlowControl User Manual O 2011 Harvard Apparatus 8 5421 002 Rev B LP Configure Pumps Before using the rest of the application functionalities FlowControl must establish communication with any pumps that will be controlled by the software To open the Pump Configuration window click on the Configure Pumps button located at the top of the screen is Pum p Configuration Legend Pump Configuration Gre Pump off line td Edt dk Wrong pump detected Pump Name Pump Model PHDULTRA O PHO ULTRA Infusion YWithdraw coms 0 PHO ULTRA 4400 InfusionMvith COM 1 When the Pump Configuration window is first launched FlowControl will automatically check for connected pumps If a pump is detected the Pump Configuration Assistant will launch see page 11 i The rest of the menu options Define Methods and Execute sp Methods will be disabled while the Pump Configuration window is active The Pump Configuration window shows the list of defined pumps to be consid
5. Syringe Models ararnnnrnnranennnnen 18 Removing Custom Syringe Models rrnnnnnnnnnnnnnnnnnrnnnnvnnnnnnnnnnnn 18 DEFINING METHODS us 19 Removing Methods nostieeia dled ei uten enConsusaibaineoned 20 Method ECON TOO Lasagne as Matson 20 Method Name Description and Dates r rarrrranrrrnnnrnnanrnnrnnnnnnn 21 Pump and Rack Model Selection rrrrrrrnnrrnrrnranrrvrnrnnnnronnrnnnnne 21 Number of Syringes Syringe Model and Flow Units Selection 22 Flow Evolution Graph Steps and Flow Definition 0 23 Visualizing Steps Step Blocks rrrrrrnnnrrrrrrnnrnrrnrrnnrrenrrnnnrrennnnn 24 GSA Eee NSG 25 Status Bar IMIOKIMAMON Guvhs detect ele cccact secede anicauncilretaveudeosswanenedse 27 Spreadsheet VIEW Ja 27 Adding New Sequential Steps to the Method rrrrrrnnnnnrrvrnnnnrn 28 Changing the Properties Of a Step rrrrrranrrnvrrnnnrrrrnrnnrnrrnrnnnnnrr 30 Inserting Steps Into a Method rrannrrnnnnrvnrnnnrnrnnnrnvrnnrnnvnnnrnnnnn 31 Removing Steps From the Method cccscccecsseeeeeeeeeeeseeeeeees 32 Defining a Loop Within a Method reerrnnrvnrrnrnerrnrrnrrrnerennnvernnnn 32 Protecting the Pump Against Forced Flow Rates 000 33 Steps Approaching Zero and Steps Changing Flow Direction 33 Expected Flow vs Real Flow ccccssscccseseeeeeseeeseeseeesaeeessaaes 33 Concentration Wizard rrnrnnnnonnnnnonrnnnerrrannenvnnnennnnnnennnnnnnnnnsnene
6. among the methods that can be executed within that specific pump rack model If the pump previously executed a method and the trial data log was not exported you will be warned that the trial data will be lost If it is accepted the method is assigned to the pump and the trial data log is cleared see page 46 When a method is selected FlowControl sets up the pump by LD sending a set of commands before starting the method execution Therefore the pump must be ready to accept such commands Please do not unplug or manipulate the pumps through their user interface while FlowControl is running FlowControl User Manual 2011 Harvard Apparatus 41 9421 002 Rev B Il Pause M Stop Method execution control Starting Pausing Resuming and Stopping methods When the execution task is entered by pressing the Execute Methods button on the experimentation assistant toolbar all methods are stopped and the information displayed is the same as when the execution task was last closed As explained previously there are start pause and stop buttons located on both the toolbar of the Method Execution window and the Method Progress that can be used to control the method The following diagram shows the different statuses of a method execution and the way it changes among them Not Started Stari Started Resumed Stop Resume Paused Stalled Na Stopped Stopping a method by either pressing the Stop bu
7. be built from a known concentration and duration All numerical values except the doses count which must be an integer value can be entered with up to 4 decimals making use of the decimal symbol currently configured in the Microsoft Windows regional settings The step sequence is generated from the data entered in the wizard and depends mainly on the option selected e Multiple Doses a step sequence is built to repeatedly infuse withdraw a volume of V ml Dose to apply mg kg Subject weight kg Concentration mg ml as many times as Number of doses states with a break time of Lag time min between two consecutive doses That means that one step is added for each dose with the following parameters Duration min V ml Flow rate ml min Start flow ml min Flow rate ml min End flow ml min Start flow Step linkage property Not linked O 000 Each of these steps is then followed by the corresponding lag step with the following parameters Duration min Lag time min Start flow ml min 0 End flow ml min 0 Step linkage property Not linked O 090 FlowControl User Manual 2011 Harvard Apparatus 35 5421 002 Rev B k Flow rate ml min NH P Step 2 Step 1 Lag time Step 3 e Duration a single step is inserted with the given duration and flow rate If the window is closed by pressing the Ok button a data verification process is automatic
8. button to move the assistant towards the last step i If the restrictions applicable to the quantity of pumps within a chain J see Compatible Network Topologies on page 6 are exceeded a warning message is shown and the assistant does not advance to the next step Selecting the mounted rack model and number of syringes The final step of the pump configuration assistant asks you to select a specific pump model from a list This will inform FlowControl of the syringe rack model being used Pump Configuration Assistant Pump Configuration Select specific pump model HU gi F sid Pi Oras vl JIS Sy Qe Max Syringe Size 140 00 ml FlowControl User Manual 2011 Harvard Apparatus 14 9421 002 Rev B The rack model list will show all of the rack models compatible with the detected pump model specific pump model must be selected in order to finish the identification process This information will be used to determine the maximum number of syringes that are mountable on the pump and the maximum size of these syringes These values are later used in the method definition to validate the maximum number of syringes and maximum size of the syringes that can be used As some racks have different restrictions depending on the number of syringes that could be used all the options will be listed separated with slash For example the 6 10 Multi Rack allows up to 10 syringes of up to 20ml or 6 syringes of up
9. by the application depending on the precision of the value Spreadsheet View A Spreadsheet View is available to display the target values of each step Click on the Spreadsheet View tab located on the top of the graph to view the spreadsheet Method Setup test Method name Modified Date Created Date Method description test ee Pump model IEEE ge of Syringes Syringe model Flow units Max method flow 57 306 ml min Cadence Science Micro Mate Glass 20 ml 1 Min method flow 56 280 nl min Graph view Spreadsheet View Start Rate Flow Step Time Acc Time Step Yolume Acc volume 10 000 ml min 00 00 07 00 00 07 1 167 mi 1 167 ml 20 000 mlfmin 20 000 mitmin 00 00 27 00 00 34 9 000 mi 10 167 mi 11 000 mlimin 11 000 mlfmin 00 00 09 00 00 43 1 650 mi 11 817 mi 11 000 ml min 19 400 mlfmin 00 00 07 00 00 50 1 773 ml 13 590 mi FlowControl User Manual O 2011 Harvard Apparatus 27 5421 002 Rev B Double clicking on a step in the spreadsheet will open the Step Editor window This allows you to edit the steps target values Adding New Sequential Steps to the Method i The Add button of the toolbar sets the graph into addition mode When the graph is set to addition mode new steps can be easily appended to the method To add a step click in the blank area of the flow evolution graph Each click will create a new step and its corresponding step block with the followin
10. icon pair of buttons and allow you to zoom in and out and thus increase decrease the visual time precision FlowControl User Manual 2011 Harvard Apparatus 26 5421 002 Rev B Time 00 00 23 Flow 15 3 mifmin Duration 00 03 56 Volume 109 3 mi Acc vol 604 9 mi Status Bar Information status bar located on the bottom of the flow evolution chart shows the following information when the mouse pointer is moved over the graph area Time corresponding to the horizontal position in which the mouse pointer is located It is shown in time format hh mm ss Flow corresponding to the vertical position in which the mouse pointer is located The flow unit value is formatted depending on how many digits are accepted by the pump model Therefore the most suitable unit will be determined to show the most accurate number possible Step duration the duration of the selected step or the step to be added as an integer value measured in seconds Step volume the partial real volume that is to be infused withdrawn within the current step up to the current time It is shown with two decimals in the most suitable volume units determined by the application depending on the precision of the value Accumulated volume the total real volume that is to be infused withdrawn by the method up to the current time It is shown with two decimals in the most suitable volume units determined
11. in the method list a suffix imported is appended to the new method name e Content method will contain exactly the same information and steps as the original saved method As a method is closely related to other elements such as syringe models and these elements could be related to others the imported method might not work exactly as the original did Please make sure that the related elements in the importation PC have the same properties as in the exportation PC If a method file could not be read or its internal structure does not match with the expected the application warns you the method file is ignored and the importation process continues with the next selected file FlowControl User Manual 2011 Harvard Apparatus 38 9421 002 Rev B When the importation process finalizes the open dialog is closed and the method table within the Methods Definition window is automatically updated FlowControl User Manual 2011 Harvard Apparatus 39 5421 002 Rev B EXECUTING METHODS Methods defined with FlowControl can be executed within the configured pump system Pa is Method Execution Execute Methods Multiple Pump Control Meth od Exec uti on p Start Selected Jf Pause Selected Resume Selected Mf Stop Selected Method Pump name Current Flow Elapsed Time Progress Duration Status Individual Pump Control i lt None gt New Pump 0 no units 00 00 00 0 00 00 00 Stopped gt Start Ml St
12. independently by means of their Close button FlowControl User Manual 2011 Harvard Apparatus 45 5421 002 Rev B Method data logging The Data Log button located on the Method Progress window provides you with the option of exporting the data resulting from the previously executed method 1 This button is only enabled for methods for which data has been I generated that is only for stopped methods When the Data Log button is pressed a Save as dialog is provided to select the destination folder and file name for the exported data Three different files are created with the same name but different extensions e Excel report xIs e Bitmap report bmp e Experimental notes txt Excel Report 1 Before exporting a data log make sure that Microsoft Excel 97 LY or higher is correctly installed on your computer If it is not installed an Excel report will not be generated A Microsoft Excel file is generated including three different data sheets that can be found on 3 separate tabs located in the bottom left corner of Excel e General This data sheet includes the following information arranged in rows Start time Name of the method executed Pump rack and syringe models used Total duration of the method execution in format hh mm ss Total volume infused accumulated positive flows Total volume withdrawn accumulated negative flows Total volume disposed by the method difference between infused
13. only allows the same characters that are allowed for a file name A method description of up to 255 characters can also be entered As informative fields Modified Date the date of the last modification and Created Date the date when the method was first created are updated automatically and are not editable Pump and Rack Model Selection A pump must be selected from the list of previously defined pumps To select a pump press the L button located next to the Pump Model field The pump s definition provides FlowControl with the following information Maximum amount of syringes mountable in the rack model Maximum and minimum speed of the pump model Maximum capacity of the pump model Pump Model Selector Please select a specific pump model from the Following list li i um Pump Model E Man Syringes Max Syringe Size Manx Steps PHD ULTRA 140 00 mi Unlimited PHO ULTRA 4400 140 00 mi Unlimited FlowControl User Manual 2011 Harvard Apparatus 21 9421 002 Rev B Harvard Apparatus of Syringes 1 v Flow units Max method flow 54 314 ml min Min method flow 52 826 nl min Number of Syringes Syringe Model and Flow Units Selection Once a pump model and a rack model are selected a syringe model must be selected among the list of compatible models A syringe model either standard or custom is considered compatible LY with a rack model when the rack model can hold syringes with a cap
14. rate if default Fails Pump Address Enter the following parameters and then press the Next button Pump Name The name that will be shown when the pump is referenced within the rest of the application s functionalities i Pump names must be unique so that two different pumps that Jy is two pumps with different connection details do not have the same name Pump Serial Number The serial number that will be assigned to the defined pump The default pump serial number for new pumps is empty COM Port The communications serial port to which the pump is currently connected The COM port list contains all the available COM ports within the computer including those simulated with a USB adapter The default COM port for new pumps is the latest selected or the first COM port available if no COM port was selected previously e To locate your COM port go to the Windows Start menu and J select Settings Control Panel System Hardware Device Manager Ports Com and LPT The ports currently being used by external devices will be listed i If no COM ports are available within the computer a warning J message is shown and the assistant will not finish the process Pressing the Configure button opens a ComPort configuration window allowing you to manually set the baud rate used for that serial port e If the new communications parameters are accepted and the pL selected COM port is used by other pumps already defined the applicat
15. Control HARVARD APPARATUS SOF T WARE FlowControl User s Manual FlowControl User Manual 5421 002 Rev B Catalog No 70 6000 HARVARD APPARATUS phone 508 893 8999 e mail techsupport harvardapparatus com web www harvardapparatus com 2011 Harvard Apparatus 1 TABLE OF CONTENTS INSTALLING FLOW CONTROL omnmnnrrnnnnnnrvnnrrrnnnnnrnnnnnnnnnnnrennrnnnnnnee 4 System Requirements rrrnnnrnnrnnnnnorrrnnnnnrnrnnnnnennnnnnnennnnnnnennnnn 4 Installing the Software rrrrnrnrrrrnnnnrrrrrnnnrenrrnnnrevnrnnnnnnnnnnnnnen 4 PUMP NETWORKS AND MODELS eennnnvevnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnnennnnnnr 6 Compatible Network Topologies rrrrrrnnrrrrrrnnrrrrrrnnnrrrnnnnnrnennnnn 6 Compatible Pump Models rrrnnnnnrnrnnnnnnvnnnnnnvrnrnnnnsrnnnnnnsennrnnnnenn 6 Pump Communications Protocols rrrrrrnnrrnrrrnrnnnrrnrnnnnrrnvrnnenne 7 FLOW CHART OUTLINING HOW TO RUN FLOWCONTROL 8 DEFINING PUMPS La 8 Pump Configuration Assistant rrrrrrnnnnnrnnrnnnnrrnrrnnrrennrnnnrrennnnn 11 Setting Pump Name and Connection Details 11 Identifying the Connected PUMP rrrnnrrnrnnnnnnrvnvrnnnnronrnnnnnsennrnsnee 13 Selecting the Mounted Rack Model and Number of Syringes 14 Removing Pumps From the System rrrrrrrnnrnrvvrrnrrrrnrrnnnrennrnnn 15 Manual Pump Controls maniare e a R 15 Defining Custom Syringe Models arrnrnnnnnnnnnnnnenrnnrrennnnnnnnnnsnen 17 Adding and Editing Custom
16. acity equal to or higher than the syringe model s capacity The following information extracted from the selected syringe model will be considered during the rest of the method edition process Syringe diameter Syringe capacity You are able to specify the number of syringes the method will make use of This value is validated so that it complies with the maximum number of mountable syringes on the rack Although it is only an informative value that is the method execution AD will consider that the pump unit has a single syringe the number of syringes is very important for the operator to be able to prepare the pump unit when the method is to be executed The flow units list allows you to choose among a list containing all possible combinations of ml ul n and p with sec min and hr The default value for the unit is selected depending on the syringe size Syringe size Default flow units 5 ul to 50 ut gt 50 pl to 1 ml gt 1 ml to 5 ml gt 5 ml to 10 mi Greater than 10 mi If different flow units are selected the flow evolution graph is automatically updated to the new flow units The syringe diameter of the selected syringe model D and the speed range of the pump model s pusher block selected Smin and Smax are considered to determine the flow rate range of the overall method These values are automatically calculated and displayed when a syringe model is selected These values are formatted depending o
17. al Pump Control I Pause stop CH Reset P Start Selected pi Pause Selected P Resume Selected E Stop Selected Method lt None gt vi lt None gt Method 1 Mixture 25 Test e Individual Pump Control Start Stop Reset a set of buttons which allow you to start pause resume stop reset the method execution on each pump independently A double click on a row which has both a pump and a method assigned automatically launches the Method Progress window The Method execution window also includes a Multiple Pump Control toolbar with the following buttons e Start Starts the execution of the selected pumps and their methods See page 42 for more details e Pause Pauses the execution of the selected pumps and their methods See page 42 for more details e Resume Resumes the execution of the selected pumps and their methods See page 42 for more details e Stop Stops the execution of the selected pumps and their methods See page 42 for more details Each of these buttons provides a drop down menu with the corresponding option Start all Pause all Resume all Stop all which will facilitate the task of starting or resuming pausing stopping all the pumps simultaneously Assigning methods to a pump The first step to be done within the Method Execution window is to assign a method to a pump A drop down list is provided on each row in the table to choose
18. ally executed checking that Subject weight gt 0 kg Flow rate is within the flow range of the method see page 22 Concentration gt 0 mg ml Number of doses gt 0 Dose value gt 0 mg kg Duration gt Command latency O O O O O 0 The verification checks that the resulting method after inserting the new steps into the existing method does not exceed the maximum quantity of steps allowed by the selected pump model If the verification is correct the step sequence or the single step is inserted just after the selected step within the method or at the end of the method if no step was selected Otherwise a warning message is shown requiring you to enter proper values A Cancel button is available within the window to ignore the changes done FlowControl User Manual 2011 Harvard Apparatus 36 5421 002 Rev B Import Export Accepting and Cancelling a Method Edit When the Accept button is pressed within the Method Editor it is closed and the changes done within the method are stored in the application s configuration However a previous information integrity check is carried out in order to avoid an inconsistent method being created The checks considered are the following e Method name duplication as stated in 0 the method name must be unique e A pump and rack model must be selected e A syringe model must be selected e Flow units must be selected If this integrity check fails you will
19. and withdrawn Flow units considered O O O O OOO O FlowControl User Manual 2011 Harvard Apparatus 46 5421 002 Rev B e Event record This data sheet includes an event record table with the following columns O O O O Current time real start time within the 24 hours of the day of the event Event time start time within the method execution of the event Event description Current flow rate the flow rate of the method at that time Total volume infused until that time accumulated positive flows Total volume withdrawn until that time accumulated negative flows Total volume disposed until that time difference between infused and withdrawn Each event will be recorded as a row in Excel table Event descriptions included within the table are the following O O O oOo O Method start User starts the method execution Step start the step has started including its name Step end the step has ended including its name Method paused the method execution has been paused whether it be manually or by the application Method resumed the method execution has been resumed Pump stalled the application has detected that a pump is stalled including its name See page 44 Comm error the application has detected a communication error See page 44 Error the application has detected an internal error See page 44 Method end the method execution has been finalized whether it be manually or by the appli
20. ation ends the modified step and the ones following it are automatically arranged in the time considering the new duration set Pressing the Esc escape key during a dragging operation will automatically cancel it so that the step will not change any of its properties i The duration of a step must always be a multiple of the command S latency of the selected pump model so that only those multiple values will be allowed e Start and end flow rates change by dragging the start and end flow markers along the Y axis Whenever the mouse pointer is located just over the flow markers it automatically changes to a vertical double arrow pointer During the dragging operation the new flow rate values are shown in the status bar see page 27 i Both the start and end flow markers are prevented from being Jy dragged out of the method s flow range Whenever the dragging operation ends the modified step is redrawn to show the new expected flow rates and the corresponding flow evolution line If the end flow of a step is changed and the following step was linked to this step the linked step will be also updated automatically start flow and flow evolution line qE ULE Wize Step linkage property change by checking unchecking the menu 2 Delete option Link to previous step available in the contextual menu of the Link to previous Step step block FlowControl User Manual 2011 Harvard Apparatus 30 5421 002 Rev B Inserting Ste
21. be warned and the Method Editor window will not be closed until changes are done or the Cancel button is pressed When the Method Editor window is closed the Method Definitions window is automatically updated to show the new information of the edited created method The information of the edited created method is automatically stored within the applications configuration and retrieved whenever the application starts again If the Cancel button is pressed you will be asked whether to discard the changes made to the method Copying a Method Copying a method allows you to take a created method and alter it without overwriting the existing method To copy a method highlight it in the Methods Definition window then press the Copy button Importing and exporting methods If you attempt to import a method while editing a different method you will be warned that you will lose your current information and will be asked to accept the importation Defined methods can be saved into external files so that they can be loaded on a different PC or on the same PC for example in case it should be recovered from a data loss Please note that the storing and retrieval options are related to the PC and not to the pump devices FlowControl User Manual 2011 Harvard Apparatus 3 7 5421 002 Rev B Import Export Export When the Methods Definition Window is open import and export commands can be found under the main Fil
22. cation e Data log This data sheet includes periodical values throughout the execution These values are obtained each minute during the method execution The following information will be logged o Elapsed Time Elapsed time since the execution started o Flow Direction Direction of the flow infuse or withdraw o Flow Rate and Units Flow rate of the pump at the current elapsed time o Accumulated Volume and Unit Accumulated volume infused withdrawn till the moment o Dispensed Volume and Unit Dispensed volume since the last log reading 1 minute FlowControl User Manual 2011 Harvard Apparatus 47 9421 002 Rev B Bitmap Report A bitmap BMP file is generated including a screenshot of the pump flow evolution chart see page 44 exactly as it appears on the window that is with the same dimensions zoom axis and content e If the Method Progress graph is zoomed in after a run you can LY zoom out to view the whole method by holding down the Control key and pressing the auto scroll button 4 Experimental Notes A text file is also created containing the experimental notes typed in the Method Progress window see page 44 At the beginning of the file a header is added containing the file name and the date and time the file was generated The file will then look similar to the following example My Data Log txt 3 16 4010 9 39 57 AM Here will be the notes added by the user in the experimental notes field
23. controlled by FlowControl e Direct Connection Each pump is connected to a single serial USB port e Daisy chain single pump is connected directly to the computer and the rest of the pumps are sequentially chained to the previous one In order to uniquely identify the pumps available within the chain each of them must be assigned an address number from 0 to 99 The limitation of how many pumps can be connected in a daisy chain depends on the pump model e PHD 22 2000 chains 20 pumps e Pump 11 Elite chains 20 pumps e PHD ULTRA chains 50 pumps a This restriction will be applied in the pump configuration tool by not AD allowing you to configure daisy chains that do not comply with the above restrictions e When communicating with a PHD ULTRA or Elite pump via USB the L pump s drivers must be installed on your PC These drivers can be found on the CD that was shipped with the ULTRA or Elite pump Compatible Pump Models The following pump models are compatible with FlowControl e PHD 22 2000 44 Protocol e 11 Elite Elite Protocol e PHD ULTRA ULTRA Protocol OEM modules may be also controlled by the application if they comply with any the ULTRA communication protocols i Max Min flow rates in FlowControl may not match up exactly to the pump s protocol FlowControl User Manual O 2011 Harvard Apparatus 6 5421 002 Rev B FlowControl is compatible with the following pump software versions and any newe
24. d edit Steps cannot be added removed modified or selected Step markers and step blocks are not shown Contextual menu is not available An automatic horizontal scroll function is provided to visually synchronize the graph and the current time e An automatic axis scale function is provided to visually adapt the graph to the current flow ranges Minimum and maximum An auto scroll button 4 is available allowing you to select whether the evolution chart is automatically scrolled or not as the method advances To manually scroll the graph click on the axis you would like to move and drag to the desired location Additionally the window shows the numerical values of the current real flow rate and the elapsed time A set of method execution control buttons Start Pause Stop are provided allowing the method to be easily controlled through the Method Progress window The functionality of these buttons is the same as those on the Method Execution window The Experimental Notes text box located at the bottom of the Method Progress window allows you to write specific details related to the trial These notes are stored in the data log which can be exported using the Data Log button The Experimental Notes field can be minimized by double clicking the button in the top left corner More than one Method Progress window can open at the same time allowing you to monitor the execution of multiple methods Each window can be closed
25. e 7 Close Pressing the Close button will shut down the Methods Definition window Removing Methods Delete When the Delete button is pressed and the operation is confirmed the set of selected methods in the table will be permanently removed Method Editor Tool The Method Editor is a powerful tool used to graphically design methods It allows you to easily draw steps with the desired flow rates iS Method Setup 123 Method name Modified Date Created Date Method description Loop properties 123 C Enable loop Pump model From Step 2 ia L To Step of Syringes Syringe model Flow units i Max flow rate 182 437 ml min Iterations 3 1 i 2 i vw Harvard Apparatus W Stainless Steel w 100ml m ov min Min flow rate 179 169 nl min Graph View Spreadsheet View Si T Flow rate ml min Method Flow Tracker 00 00 00 00 00 05 00 00 10 Time hh mm ss Time 00 00 00 Flow 0 000 pl hr FlowControl User Manual 2011 Harvard Apparatus 20 5421 002 Rev B Method name Modified Date Created Date puan pe Method Name Description and Dates To create a new method click the Define Methods button located on the experimentation assistant bar In the Methods Definition window press the Add New button to bring up the Method Setup window Enter a unique name for the method In order to facilitate the exportation and importation tasks see page 37 the method name
26. e following format lt COM port name gt lt Pump address gt By default table rows are sorted by connection that is by COM port and address Sorting can be changed by clicking on the column headers of the table The Pump Configuration window includes a toolbar with the following buttons e Add New Launches the Pump Configuration assistant tool in order to define a new pump This button is enabled only if the total quantity of defined pumps has not reached the limit e Edit Launches the Pump Configuration assistant tool in order to modify a previously defined pump This button is enabled when one and only one pump is selected e Delete Asks the user to confirm whether to remove the selected pump s This button is enabled when at least one pump is selected See Removing Pumps from the System on page 10 for more details FlowControl User Manual 2011 Harvard Apparatus 10 9421 002 Rev B b Pump control Pressing the Refresh button of the window updates the status of all the currently defined pumps To do that the application establishes communications with each of the defined pumps in order to determine their current availability that is whether they are currently connected to the computer or not and to obtain their corresponding pump model 1 When connecting a new pump model use the Add new function Do not attempt to edit an existing connection for a new pump model Pressing the Pump Control butto
27. e list of commands sent to the pump The Close button enables you to close the Manual Pump Control window while the pump is running This allows you to open another Manual Pump Control window and control other defined pumps Pumps that are running will be highlighted in green in the Pump Configuration window The Stop and Close button stops any started operation and closes the Manual Pump Control window FlowControl User Manual 2011 Harvard Apparatus 16 5421 002 Rev B Configuration Custom syringes Defining Custom Syringe Models In addition to the pre configured syringe models FlowControl allows you to define your own custom syringe models The Custom Syringes window is available through the Configuration menu option with the same name Under Configuration choose Custom Syringes to bring up the following window Custom Syringes Custom Syringes Custom Syringe Name Inside Diameter mm 15 000 mi 30 000 mi The Custom Syringes window shows a list of custom syringe models that have been defined These syringes are available within the rest of FlowControl s functions Such as method creation Each custom syringe model is represented by a row with the following information columns e Custom Syringe Name The name assigned to the custom model during its definition e Size The total capacity ml or ul entered during its definition e Inside Diameter mm The inside diameter of the syringe s bod
28. e tab When one or more methods are selected within the methods table the File Export menu option will be enabled If that option is then selected a standard save dialog will be shown allowing you to choose a destination folder and file name in which to store each of the method files If a folder and file name are successfully selected and the dialog is accepted the application generates the file whose content will include all the information needed to rebuild the method when it is opened including the original name of the method If the file to be generated already exists a warning will be shown and you will be asked to select whether or not to replace the existing file Anyway the saving process will continue with the following selected method The File Import menu option allows you to load a set of method files previously exported by the application To do this a standard open dialog is shown allowing you to choose a source folder and the set of files If this dialog is cancelled no method will be opened so that the current method list will not be altered and the opening process automatically ends If you accept the opening a new method is automatically added to the method list for each of the selected method files with the following characteristics e Method name the original name of the method when it was saved It could be different from the name of the method file opened If a method with the same name already exists
29. ered within the rest of the application s options such as Method creation and execution Each defined pump is represented by a row in a table with the following information e Status shows an icon representing the connectivity of the pump o Green Ready The pump is correctly defined and connected to the computer o Gray Off line The pump was correctly defined but it is not currently connected to the computer o Red Wrong The pump model currently connected to the computer does not match with the defined pump FlowControl User Manual O 2011 Harvard Apparatus 9 5421 002 Rev B Note that if you close and restart FlowControl pumps 1 connected via a USB cable may re establish communication through a different COM port than was previously defined Therefore if multiple pumps are defined they could potentially change COM ports during a re start Be sure to verify which pump you are communicating with before running a method Except for green status the row color text foreground color coincides with the status icon color in order to facilitate its visual identification e Pump Name shows the name assigned to the pump during its definition e Pump Model shows the name of the pump model detected by the software e Serial Number shows the serial number of the pump entered during its definition e Communication shows the communications port name COM port and the address of the pump entered during its definition in th
30. expected Start flow rate value An end flow marker a rectangular solid green marker located at the right side of the step block and at the vertical position representing the expected end flow rate value A flow evolution line connecting the start and end flow markers and representing the expected flow evolution FlowControl User Manual 2011 Harvard Apparatus 24 5421 002 Rev B Y ax1s I flow Expected flow evolution line Start flow marker X axis time Step block Step block Step block Duration marker The first step block is always located at the left most side of the graph plot As no time gaps are allowed between two steps the following blocks will be drawn next to the previous one Step blocks can be easily selected by clicking on them with the left mouse button when in selection mode Graph s Axis The flow evolution graph includes a pair of orthogonal axis representing the time x axis and the flow rate y axis X axis has the following characteristics Values are measured and shown in time format hh mm ss The minimum value is always 00 00 01 The maximum value is determined by the end time of the last step defined within the method Whereas no steps were defined a default maximum value of 30 minutes is applied Time precision is the same as the command latency of the selected pump model FlowControl User Manual 2011 Harvard Apparatus 25 5421 002 Rev B an Y axis has the fol
31. g information Check the graph s scale before adding steps To adjust the scale use the G buttons Expected start flow Same as the last steps expected end flow The first step in a method will be added as a constant rate whose flow rate is determined by the cursor s position when created Expected end flow Corresponding to the cursor s vertical position when the click was made Duration Corresponding to the cursor s horizontal position when the click was made Step linkage property By default all new steps added are considered as linked to the previous one When a new step is added the method is automatically recalculated so that its overall information is updated within the status bar FlowControl User Manual 2011 Harvard Apparatus 28 5421 002 Rev B ey Properties FE Insert 141 T L Lin III When adding a new step the Step Editor window will automatically open allowing you to edit the step s target values duration flow values and step linkage property This window can also be accessed by clicking on the Properties icon in Method Setup or by right clicking on a step and selecting Properties Step editor Step 4 Step values Accumulated values volume 21 649 Start flow w Same as end flow of previous step end flow 16 300 Duration 00 01 23 hhimmiss Changing the flow may affect the duration or volume of the step Therefore the following rules will be appl
32. ied when the step volume start end flow rates or the duration are changed e If the volume changes then the duration time will be updated e If the duration time changes then the volume will be updated e If the start end flow changes then the volume will be updated Additional editable information is available in the Accumulated values tab page of the Properties window e Volume accumulated real volume infused withdrawn by HOUSE the method until the end of the current step If the value is changed then the step volume volume and thus the step duration and i Time hh mm ss the accumulated time will be automatically updated a Step values Accumulated values e Time total method duration until the end of the current step If the value is changed then the my meeen step duration and thus the step duration and volume and the accumulated volume will be automatically updated FlowControl User Manual 2011 Harvard Apparatus 29 5421 002 Rev B Changing the Properties of a Step The following properties of a defined step may be changed using the flow evolution graph s visual elements e Duration change by dragging the duration marker along the X axis Whenever the mouse pointer is located near to the duration marker it automatically changes to a horizontal double arrow pointer i The duration marker is prevented from being dragged before the ev step s start time When the dragging oper
33. ion warns you that the new parameters could affect other pumps An automatic baud rate detector tool is provided by FlowControl to facilitate the task of configuring the communication settings This tool is activated when the option Try to detect baud rate if default fails is checked Pump Address The address number assigned to the pump within the communications chain The default pump address number for new pump is one more than the highest address within the previously defined pumps in the same chain that is configured with the same COM port or 0 if the first pump is being defined FlowControl User Manual 2011 Harvard Apparatus 12 9421 002 Rev B If the assistant was launched to modify an existing pump fields will contain the previously entered information for that pump Pressing the Cancel button will ask you whether to terminate the definition process ignoring all the information provided since then Cancelling the definition process will close the assistant without creating any new pump or modifying any of the pumps already defined Pressing the Next button makes the assistant move towards the next step Before doing this the assistant checks that the information provided is suitable The following information is checked o Pump name is not empty o Pump name is unique that is no other defined pump has the same name o A COM port is selected Pump address is a number between 0 and 99 o Pump address is u
34. led selected within the Pump Configuration window The syringe model selector contains the list of mountable syringe models within the selected pump model both standard and custom syringe models By default the first syringe model in the list is selected When a syringe model is selected its diameter is shown and the flow range of the pump is automatically calculated and displayed based on the speed range of the pump model and the syringe diameter The flow rate edit box allows you to enter the desired flow rate positive values in the flow units selected Flow rates can be changed while the pump is running y The flow rate value must always be within the flow range calculated LD If it is out of bounds it will be automatically changed to the maximum or minimum of the range The flow rate units may be specified from a combination of volume units ml ul nl and pl with time units sec min and hr Use the set of control buttons gt and Stop to make the pump to infuse withdraw the selected flow rate and to stop the pump i The flow rate edit box will be enabled only when a syringe model is L selected Control buttons will be enabled whenever a valid flow rate value is entered While a pump is being controlled manually FlowControl will be responsible for sending the corresponding commands to the pump in order to start and stop the infusion withdrawal operations A Command log area displays th
35. lity to resume stop Pump answers Unknown command answer same timeout as command latency Severe internal data inconsistency Method is stopped Comm Error Method Progress Window In addition to the runtime execution information shown within the Method Execution window FlowControl provides you with a tool to graphically monitor the real flow evolution of each independent pump Method Progress PHD ULTRA O COM 0 ramp Running i Stainless Steel 50 mi 50 741 ml min 41 517 m 1 s m a Target 44 250 mi Flow direction gt Time Elapsed 00 00 14 3 l Time Remaining 00 00 27 Duration 00 00 42 mo Method Progress Flow rate ml min PHD ULTRA 0 COM 0 ramp OG 00 00 00 00 10 00 00 20 00 00 30 00 00 40 Time hhimm ss Time 00 00 00 Flow 0 000 ply hr Experimental Notes FlowControl User Manual 2011 Harvard Apparatus 44 5421 002 Rev B The Method Progress window is opened by double clicking on the pump s corresponding row in the Method Execution table The pump and method name are displayed in the title bar of the Method Progress window in order to facilitate visual identification of each window which shows the method s flow evolution graph The flow evolution graph shows the evolution of the real flow along the time combined with the expected flow set during the method edit task However the following functionalities differ from the flow evolution graph used during the metho
36. lizes an important amount of PC resources and can be highly time consuming if the computer used is not powerful enough Thus Harvard Apparatus recommends the use of a computer that meets the following minimum requirements 1 2 GHz Pentium processor or higher 2 512 MB of RAM 1 GB recommended 3 Windows 7 XP SP3 or Vista XP recommended 4 Free RS 232 or USB 2 0 ports depending on the quantity model and connectivity of the controlled pumps Daisy chained pumps require a single port Direct PC to Pump connections require one free port per pump 5 Microsoft Excel 97 or higher Installing the Software Insert the provided USB Flash Drive into your PC Open the drive titled PBLicense Click on the FlowControl Setup executable to launch the installation wizard FlowControl InstallAware Wizard Welcome to the Install ware Wizard For FlowControl Setup This wizard will install FlowControl on your computer It is strongly recommended that you exit all Windows programs before running this Setup Programm Click Cancel to quit Setup and close any programs you have running Click Next to continue with the Setup program WARNING This program is protected by copyright lav and international treaties Unauthorized reproduction or distribution of this program or any portion of it may result in severe civil and criminal t penalties and will be prosecuted to the maximum extent possible under law FlowControl Use
37. lowing characteristics Values will be measured and shown in the flow units selected by the User The maximum value always positive will be determined by the maximum flow between those defined by the User within the steps of the method It will be also limited by the maximum flow allowed by the selected pump and syringe models see page 22 The maximum flow will be represented by a horizontal line with a tag showing the value The area above this maximum flow will be shaded gray The minimum value always negative will be automatically set to the absolute value of the maximum flow with the negative sign whenever the maximum value is changed The minimum flow will be represented by a horizontal line with a tag showing the value The area below this minimum flow will be shaded gray The area around zero below the absolute value of the minimum flow that the pump is capable of performing will be indicated by two horizontal lines The area between these lines will be shaded gray Furthermore the value represented by the line will be drawn on top of each line aligned to the right It will be crossed by the X axis always at flow rate 0 Zoom options are available to allow the User to increase decrease the visual flow precision Both the X and Y axis provide a drag and drop mechanism that allows you to freely move along the time the flow range To use it drag the zone near to the axis values when the mouse pointer changes to a double arrow
38. n how many digits are accepted by the pump model Therefore the most suitable unit is determined to show the most accurate number possible FlowControl User Manual 2011 Harvard Apparatus 22 5421 002 Rev B za Flow Evolution Graph Steps and Flow Definition The flow evolution graph area shows a graphical representation of the defined method s steps It works as a canvas in which you can draw your own step sequence and the desired flow rate evolution lines it SHEMX 2 Flow rate ul min Method Flow Tracker 900 1000 0 1000 2000 00 00 00 00 05 00 00 10 00 00 15 00 00 20 00 Time seconds a Time 00 00 00 Flow 0 pl hr Duration 00 08 15 Volume 18562 500 ul Acc Vol 21887 500 u The flow evolution chart area provides a toolbar to facilitate the execution of the most common tasks related to the method edit process The following buttons are included in the toolbar e Select Sets the graph into selection mode allowing you to select or modify steps but not create new ones see page 27 e Add Sets the graph into steps addition mode allowing you to add new sequential steps into the method see page 28 e Properties Allows you to change the properties of the selected step see page 29 e Insert Adds a new step in the middle of the sequence see page 31 This button is enabled when the total quantity of steps has not reached the predefined limit of the selected pump model as defined in 0 e C
39. n launches the Manual Pump Control tool for the selected pump This button is only enabled when one defined pump with green status is highlighted Refer to page 15 for more details Pressing the Accept button closes the Pump Configuration window and stores the defined pumps list so that it is considered within the rest of the application s functionalities Pressing the Cancel button of the window asks the user to confirm whether to ignore the changes made within the list of defined pumps so that the application s configuration is not altered Only if you accept the confirmation will the Pump Configuration window close If no changes were made to the list of defined pumps the confirmation message is not shown Pump Configuration Assistant In order to facilitate the definition of new pumps and the modification of defined pumps FlowControl provides the Pump Configuration Assistant which arranges the process into three steps Setting Pump Name and Connection Details To define a new pump in the Pump Configuration screen press the Add New button this will launch the Pump Configuration Assistant Pump Configuration Assistant Pump Configuration Pump Name New Pump Serial Number Com Port COM1 vi Pump Address 0 Try to detect baud rate if default Fails FlowControl User Manual 2011 Harvard Apparatus 11 9421 002 Rev B Pump Mame 18995 Serial Number fs Com Pork COMS Ww Try to detect baud
40. nique within the chain that is no other defined pump has the same COM port and the same pump address O 1 If one or more of these conditions are not fulfilled the assistant will Hy not move towards the next step In that case you must make the necessary corrections before continuing with the assistant Identifying the Connected Pump The second step of the pump configuration assistant is to execute the automatic identification process of the defined pump Pump Configuration Assistant Pump Configuration Pump Identification Pump Identified mr Start identification Press the Start identification button to determine the pump model Kop Start identification connected to the serial port with the address number specified in the previous step FlowControl User Manual O 2011 Harvard Apparatus 13 5421 002 Rev B Establishing communication with pump An activity bar serves as a visual signal of the progress of the identification process At the end of the identification process the assistant shows the result indicating the detected pump model name and version i The automatic identification process requires the pumps to send the Jy version identifier For PHD ULTRA and Elite pumps please check that the echo function is disabled before trying to identify them In order to disable the echo function please refer to the PHD ULTRA or Elite User s Manual Once the pump has been identified press the Next
41. odified Date Created Date Method description Loop properties 123 Enable loop Pump model From Step 2 To Step 4 of Syringes Syringe model Flow units Mat Dog cate DAT Grane D Min flow rate 179 169 nl min 1 Harvard Apparatus W Stainless Steel Y 100ml w ml YY min Graph View Spreadsheet View Method Flow Tracker 00 00 00 00 00 05 00 00 10 00 00 20 Time hh mm ss a Time 00 00 00 Flow 0 000 pl hr FlowControl User Manual 2011 Harvard Apparatus 32 9421 002 Rev B Protecting the Pump Against Forced Flow Rates FlowControl does not consider the maximum forces applicable to AN each of the pump models You must configure the pumps to prevent them from going beyond their maximum forces through their own user interfaces Steps Approaching Zero and Steps Changing Flow Direction Steps that approach a flow rate of zero steps which have a start or end flow equal to zero have to consider that there is a minimum flow rate that the pump model can not handle Therefore if a step approaches zero flow the ramp will not actually finish at zero flow but at the minimum possible flow rate and then drop to zero This applies from positive and negative to zero and from zero to positive or negative When a step is defined to start or end with a flow rate lower than the minimum allowed by the pump model the expected flow evolution line will be automatically recalculated to start end exactly with that minimum flo
42. oncentration Wizard Allows you to easily insert a step sequence from a known target concentration and dose see page 34 e Delete Asks the User to confirm the removal of the selected step see page 32 e Autoscale Automatically adjusts the chart area to show all the steps in the sequence FlowControl User Manual 2011 Harvard Apparatus 23 9421 002 Rev B The flow evolution graph also provides a contextual menu which is dropped down when the right mouse button is pressed over the graph area This menu facilitates the access to the following options Properties Insert Concentration wizard See page 34 for more details on this option Delete Link to previous step See page 30 for more details on this option Visualizing Steps Step Blocks Each step of the method is represented as a rectangular region called step block with the following characteristics It is drawn over the rest of the graph elements Its width is proportional to its duration Its left side coincides with its starting time of the step Its background color alternates between two light colors in order to facilitate identification Each step block includes the following elements related to the step s defined information duration marker shown as a vertical line located at the right side of the region A start flow marker a circular solid blue marker located at the left side of the step block and at the vertical position representing the
43. oncentration wizard 1 5000 kg 0 500 Flow units ml 0 2500 mg m Animal weight Flow rate Concentration Multiple doses 2 0000 0 00 15 pal Dose to apply Lag time Single dose Duration The following information must be entered to set up a concentration step Animal weight the weight always in Kg of the subject to from which the fluid will be infused withdrawn Flow rate the expected flow rate to be applied Flow rate units the units of the flow rate The possible volume units are ml ul nl and pl and the possible time units are sec min and hr Concentration the concentration of the fluid to be infused withdrawn always in mg ml Number of doses the amount of doses to be infused withdrawn Dose to apply the dose to be infused withdrawn always in mg kg Lag time amount of time to wait between each dose infusion withdrawal in time format hh mm ss Duration the duration always in minutes of the single step to be inserted The default value will be 1 min 2011 Harvard Apparatus 34 9421 002 Rev B 8 Multiple doses Single dose The number of doses dose value and lag time fields are available only if the Multiple Doses option is selected This means that a step sequence is to be built from a known dose and concentration The duration value field is available only if the Single Dose option is selected This means that a single step is to
44. op Es Reset lt None gt 22 0 no units 00 00 00 00 00 00 Stopped P Start MM Stop E Reset To do that a floating window called Method Execution is provided through the Execute Methods button located in the experimentation assistant bar The available pump list table shows the pumps already defined within the pump system together with the method to be executed within each of them Each row of the table shows the following information Method e Method the name of the method which will be executed within the Test pump see page 19 Pump ee e Pump name the name assigned to the pump during its creation Er edit process see page 11 Current Flow e Current flow the real flow infused withdrawn by the pump currently It is shown with 3 decimal places and an W character showing whether a infusion I or a withdrawal W is being done see page 43 I 16 208 nilmin Elapsed Time 00 00 17 e Elapsed time the time in hh mm ss format lapsed from the beginning of the method execution see page 43 Progress Progress a bar showing the percentage of the method which has already been executed It is calculated as Current time Duration ti Buran e Duration the total duration of the method 00 01 53 en e Status the current execution status of the pump see page 44 alus Running FlowControl User Manual 2011 Harvard Apparatus 40 5421 002 Rev B Individu
45. pacity is measured The units options include o mi milliliters o ul microliters e Inside diameter The inside diameter of the syringe s body always inmm The maximum value for the diameter is 400mm The assigned syringe capacity is used by the Method Editor tool to determine the compatibility between the defined custom syringe model and the rack models Removing Custom Syringe Models Pressing the Delete button when one or more defined custom syringes are selected will ask the user whether to remove the selected models from the list permanently FlowControl User Manual 2011 Harvard Apparatus 18 9421 002 Rev B h Define Methods DEFINING METHODS Methods are the procedures by which a set of pumps execute a sequence of pre defined steps and thus a particular flow rate is applied FlowControl allows you to manage your own methods through the Methods Definition window which is accessed by means of the Define Methods button located in the experimentation assistant bar iS Methods Definition Methods Definition Pump Model of Steps Total Duration Total Yolume Modified Pump 11 Elite Pico Infusi 6 00 00 22 50 010 ul 6 2 2011 PHD ULTRA InfusionMWi 01 00 29 55 59 833 mi 6 2 2011 PHD ULTRA 4400 Infusi 3 00 01 45 4 167 mi 6 2 2011 The methods table shows the list of methods already defined Each row of the table shows the following information columns e Method Name The name assigned to the me
46. ps Into a Method The Insert button of the toolbar and its equivalent option within the HE contextual menu of the selected step allows you to add a new step just EE Properties before the currently selected step This button is enabled when a step is 20 Insert selected and when the total quantity of steps has not reached the predefined JA anranteabinn d limit of the selected pump model The inserted step will have the following properties e Expected start flow Same as the expected start flow of the selected step e Expected end flow Same as the expected start flow e Duration Same as the selected step s duration e Step linkage property Same as the selected step s linking property The following screenshots illustrate the step insertion process in both cases linked and not linked steps Case 1 Linked steps Inserted ul min ulmin 6000 4000 4000 3000 Inserted Ste 2000 2000 P 1000 0 0 00 00 00 00 10 00 00 00 00 00 10 00 Case 2 Steps not linked ulin ulfmin Inserted Step 4000 3000 2000 1000 00 00 00 00 10 00 00 00 00 00 10 00 In Case 2 in which the selected step is not linked to the preceding J the inserted step is not linked either but the selected step changes its linking property to be automatically linked to the inserted FlowControl User Manual 2011 Harvard Apparatus 31 5421 002 Rev B ad Jeg LoOncentration wierd Delete
47. r Manual O 2011 Harvard Apparatus 4 5421 002 Rev B Press the Next button to move to the Destination Location screen Select the location that you would like FlowControl to be saved on your PC then press Next In the specified location FlowControl will download an executable file and a reference database FlowControl InstallAware Wizard Destination Location a Setup will install FlowControl in the Following Folder E Desktop EH My Documents Ei yf My Computer eee Local Disk 0 Sy AMD EMO bbf 56caa aef994eb850037 Ei cabs Destination Folder C Program Files Harvard ApparatusiFlowControl 4 072 KB 13 242 MB Required Disk Space Disk Space Remaining Once successfully installed you will have the option to run FlowControl immediately To do so click the dialog box Run FlowControl Now and then press the Finish button FlowControl InstallAware Wizard E E FlowControl has been successfully installed Click he Finish button to exit this installation Finish k J The USB Flash Drive contains the FlowControl software license and must be plugged into the PC whenever FlowControl is in use FlowControl User Manual 2011 Harvard Apparatus 5 5421 002 Rev B PUMP NETWORKS AND MODELS FlowControl is able to communicate with a pump connected to a computer by means of a serial USB communications protocol Compatible Network Topologies The following pump network topologies can be
48. r revisions PHD 22 2000 vipi2a Pump Communications Protocols FlowControl is responsible for selecting and fulfilling the communications protocol suitable for each pump with which it will communicate The communications protocol of each model that the application will utilize is described in the pump s corresponding user manual FlowControl uses the following command latency for each pump PHD 22 2000 PHD ULTRA FlowControl is thus responsible for sending the corresponding commands to each pump from their particular commands set in order to control them 1 Within a daisy chain network topology all the pumps must be LL configured by the user to have the same communication parameters baud rate USB based pumps are controlled by FlowControl through a virtual JU serial port automatically created by the pump s driver Thus all pumps are considered to be connected to the PC by means of a serial port virtual or physical FlowControl User Manual O 2011 Harvard Apparatus 7 5421 002 Rev B Running FlowControl d me GE BX et pump name and connection details Add Custom Syringe Define a Pump Pest connected pump El g 7 Select rack model and of syringes all OM F N Set method name description and dates Manual Pump Control Define a Method Select pump model of syringes and flow units Ne i Add I edit steps Edit a method F wN Assign method to a pump
49. thod during the creation edit process e Pump Model The model name of the pump in which the method is intended to be executed e of Steps The number of steps in the method e Total Duration The total duration in hh mm ss of the defined method e Total Volume The total volume of fluid that will be infused withdrawn by the method at the end of its execution expressed in the default units for the pump model selected e Modified The date when the method was last modified FlowControl User Manual 2011 Harvard Apparatus 19 5421 002 Rev B The methods table allows you to select multiple methods in order to apply the same operation to all them simultaneously You can sort the table rows by clicking on the column headers A double click on a row of a method automatically launches the Method Editor tool The Methods Definition window also includes a toolbar with the following buttons dh Add new e Add new Launches the Method Editor tool in order to create a new method Edit e Edit Launches the Method Editor tool in order to modify an existing i method This button is enabled when one and only one method is selected Copy e Copy Creates a copy of the selected method which can be renamed and altered to create a new method Delete Asks you to confirm whether to remove the selected method s This button is enabled when at least one method is selected and all the selected methods could be removed Delet
50. to 60ml Pressing the Finish button will finalize the identification process closing the assistant and adding a new record into the pumps list of the Pump Configuration window Removing Pumps From the System Pressing the Delete button within the Pump configuration window when a defined pump or set of pumps are selected will ask you to confirm that the selected pumps should be removed from the list i Removed pumps will not be available within the rest of the y application s functionalities e g method definition and method execution Manual Pump Control The Manual Pump Control tool PHD ULTRA O COM9 0 Manual Pump Control allows you to carry out basic PUMP synge pa CO ntrol ope ratio n S O n th e se ected Select the syringe model mounted in the pump PU mp by means of the following apparans v Stainless Steel m 25 mi j window Ve se Max Flow 3 525 ml min To enter Manual Pump Control press 77 Flow rate Flow units the Pump Control button located at the 5 02 Im Mmm bottom of the Pump Configuration window 2 000 ul min The rest of FlowControl s at functionalities menu options experimentation assistant bar etc are disabled while this window is active FlowControl User Manual O 2011 Harvard Apparatus 15 5421 002 Rev B E Stop ug i Stop and close The title bar of the window shows the name COM port and address of the pump to be control
51. tton or by the method end implies the closing of communication with that particular pump Therefore no more commands will be sent to the pump and no answers coming from it will be processed However paused methods will not close the communication channel the pump will remain controlled by the computer but commands will not be sent while paused FlowControl User Manual 2011 Harvard Apparatus 42 5421 002 Rev B Runtime Execution Information Whenever a set of methods is being executed on a group of pumps FlowControl will manage the communications to The automatically updated by FlowControl Send the appropriate commands to each pump depending on its model Set the real flow to each pump depending on the step sequence of the associated method see page 23 Detect the special conditions stalling errors etc of each pump Determine the current flow of each pump following information related to the execution control task is Method Execution Multiple Pump Control Meth od Exec uti on p Start Selected Qf Pause Selected gt Resume Selected Mf Stop Selected Duration Status Individual Pump Control Method Pump name Current Flow Elapsed Time Progress Ramp 0 000 no units 00 00 00 00 00 22 Ready to run gt Start M Stop 69 Reset Method 1 Remote 0 000 no units 00 00 00 00 02 00 Readytorun Start m stop Reset Current flow matches the real flow value infused
52. w rate but keeping the defined duration Positive flow Infuse Real flow Minimum flow Expected flow rata Negative flow Widthraw Expected Flow vs Real Flow As stated before the Method Editor tool allows the User to define the expected flow rates that the pump should apply throughout the method However the particular pump model for which the method was designed and thus in which the method is intended to be executed will determine the real flow that will be finally applied When a method is running FlowControl is responsible for translating the expected flow rates for the particular pump model in which it is executed so that the differences between expected flow rates and real flow rates are minimized FlowControl User Manual 2011 Harvard Apparatus 33 5421 002 Rev B Z POpercies ER Insert FEN Concentration Wizard p Delete I ir le ka Publ MT _ T ker 1000 Animal weight Flow rake a 0 500 0 2500 mg ml Number of doses z 0000 mg kg Concentration Dose to apply Lag time 00 01 30 Duration io min Concentration Wizard A Concentration wizard is available to facilitate the task of designing a step sequence or a single step from a known target concentration and dose This tool is accessible both through the flow evolution toolbar and from the Concentration wizard option in the contextual menu of the chart FlowControl User Manual C
53. withdrawn by the pump at that time which could not match with the expected flow set within the Method Editor see page 33 The current flow will be updated on the screen every 500 msecs Elapsed time is updated every second to show the time lapsed from the beginning of the method execution discarding the time that the method had been paused Progress is updated as Elapsed time changes to show the percentage in a visual form of the method s duration that has been executed The percentage is calculated as the quotient between Elapsed time and Duration Status When special situations arise a text is shown to identify them o Paused the method is manually paused o Stalled the pump is stalled and thus the method is paused by FlowControl o Comm error an error on the communications channel has been detected o Error an unknown error on the system has been detected FlowControl User Manual 2011 Harvard Apparatus 43 5421 002 Rev B Controlling Special Situations During the Method Execution The following table shows the events that are considered by the application in order to detect specific situations and the reaction it will have against them Event Situation Reaction User presses Pause button Method is paused Pump answers stalled Stalled Method is paused User is warned p and given the possibility to resume stop Method is paused User is warned and given the possibi
54. y always in mm entered during the definition By default the table rows are sorted by model name but the sorting method can be changed by clicking on the column headers of the table FlowControl User Manual 2011 Harvard Apparatus 17 9421 002 Rev B The Custom Syringe window also includes a toolbar with the following buttons e Add Launches the editor tool in order to define a new custom syringe model e Edit Launches the editor tool in order to modify a previously defined custom syringe model This button is enabled when one and only one syringe is selected A double click on a row of a defined custom model automatically launches the editor tool e Remove Requesis confirmation to remove the selected custom syringe models This button is enabled when at least one custom model is selected Adding and Editing Custom Syringe Models Pressing the Add new or Edit buttons from the Custom Syringes window automatically launches the editor tool which allows you to create new custom syringe models or modify the parameters of an existing one The following information for each custom syringe model must be entered e Custom syringe name The name of the custom syringe model which will be used within the rest of the application s functionalities Custom model names must be unique e Size value The total volume of fluid that the syringe can hold The maximum value possible is 140ml e Size units The volume units in which the ca
Download Pdf Manuals
Related Search