ÄKTAcrossflow - GE Healthcare Life Sciences
Contents
1. CURVE AMPLITUD CHANGE TEST RESULT R VB Outl OK Minimum amplitud he change in amplitude was 1 82000 bar change allowed is 1 00000 bar CURVE AMPLITUD CHANGE TEST RESULT R VB Out2 OK AKTAcrossflow Installation Guide 11 0012 35 Edition AD The change in amplitude was 1 41000 bar 53 6 Installation test 6 4 Evaluating the installation test results 54 Minimum amplitude change allowed is 1 00000 bar CURVE AMPLITUDE CHANGE EST RESULT R VB Out3 OK The change in amplitude was 1 89000 bar Minimum amplitude change allowed is 1 00000 bar CURVE AMPLITUDE CHANGE 4 EST RESULT UV sensor OK The change in amplitude was 111 74500 mAU Minimum amplitude change allowed is 85 00000 mAU CURVE AMPLITUDE CHANGE H EST RESULT Cond sensor OK The change in amplitude was 101 54900 mS cm Minimum amplitude change allowed is 70 00000 mS cm CURVE AMPLITUDE CHANGE 4 EST RESULT pH sensor OK The change in amplitude was 3 91000 Minimum amplitude change allowed is 2 00000 Logbook Event test result Air sensor OK CURVE AMPLITUDE CHANGE EST RESULT R PCV P PCV OK The change in amplitude was 0 66000 bar Minimum amplitude change allowed is 0 17000 ba2. Ethernet Fig 5 8 Ethernet communication cable One end of the Ethernet communication cable is connected to AKTAcrossflow at delivery Connect the other end to an Ethernet communication port 5 10 CU 950 configuration For instructions on configuration of the CU 950 refer to AKTAcrossflow Service Manual The mechanical and electrical installation phase of AKTAcrossflow is now complete 34 AKTAcrossflow Installation Guide 11 0012 35 Edition AD Installation of AKTAcrossflow 5 5 11 Connecting Frac 920 optional to AKTAcrossflow 5 11 1 Connecting to mains supply To locate the Mains connector on Frac 920 see Fig 5 9 below Note Frac 920 is tilted to allow access to the rear panel Frac 920 Rear panel ea o Go o Go Drop Monitor Counter Valve Event mark Remote UniNet 1 Drop Valve Auxiliary Remote UniNet 1 Mains sensor equipment digital I O Fig 5 9 Frac 920 connectors Note Frac 920 contains no user replaceable fuse AKTAcrossflow Installation Guide 11 0012 35 Edition AD 35 5 Installation of AKTAcrossflow 5 11 Connecting Frac 920 optional to AKTAcrossflow To connect Frac 920 to AKTAcrossflow do as follows 1 Remove the instrument s top cover by first sliding it towards the front panel and then lift up Top cover 2 Using the two handles remove the rear panel by first moving the handles sidewards to the left and then lift out the panel
3. Handles CAUTION The free mains outlet inside the AKTAcrossflow instrument is intended for connection of the fraction collector Frac 920 only 36 AKTAcrossflow Installation Guide 11 0012 35 Edition AD Installation of AKTAcrossflow 5 3 Connect the supplied mains cable between Frac 920 and the free mains socket inside the AKTAcrossflow instrument HW GIGGGE Free mains outlet for con nection of Frac 920 CU 950 and VAP 980 mounting plate eo eo J a Frac 920 mains cable Note The illustration above shows the mains outlet from inside the AKTAcrossflow instrument with the rear panel removed Note For easy access inside the instrument the CU 950 and VAP 980 mounting plate is suspended pivotally AKTAcrossflow Installation Guide 11 0012 35 Edition AD 37 5 Installation of AKTAcrossflow 5 11 Connecting Frac 920 optional to AKTAcrossflow 5 11 2 UniNet 1 communication CAUTION The mains power to the AKTAcrossflow system and the PC must be switched off before connecting the instrument to the UniNet 1 link To include the fraction collector in the UniNet 1 communication chain do as follows 1 Remove the right hand side cover by first looseni
4. Gace Meted cemedty Am s Dadaan RT 525 OSD 22 Lons Espen Sti Tmel Canedo gma ome sent deco Matrox int matte ty Aer i mitezza ONT O35 CRARIE Cee Cowen Redes Yo Sauna remer any cow Snoey we O12 min Emgn Rerevor RVOR 300 ber 6000 myn 012 min Holel_Uni EmphyFesFenshed Equal 1 init riri _0 19 min End 2007 05 25 09 43 11 Cantini European Standard Time Manual USI U Eni No nerd Conroled Bei Arda Fig 6 8 Information on Method selected AKTAcrossflow Installation Guide 11 0012 35 Edition AD Installation test 6 9 Inthe final Result Name dialog a result name can be entered and the file storage location can be edited O Syrin Cantr ih Cremations t Semi attest Wizard ener ated banah 30 Rui ford ontinny w je Bie Cue DREI ee fork fent AM arae deter io ent nane dress ar 1 07 min Base Tim 0 12 min End Block 0 12 min End Black 0 12 min EmpyPaservorYE Ov2 2 00bar 6000 mymin 0 12 min Hold Usi EmphResFewated Eque 1 Inteute ran 0 73 min Ei 06 28 08 49 11 Corral European Stendard Time Marsa Fa neo z Fig 6 9 Result name dialog To start the Installation test click START rassflow1 Saniltx ard Fie Ven Manuel System nep Run Hold ntinve End dO ww leee e oreEeE aa aa a eae i Instruments Connection Nun Status Acc Voima Block Volume Ace Tine Block Time TifFlow gt a a CC Ce ee ee a T ae gt nr RefVol gt gt Resa gt gt
5. AKTAcrossflow Installation Guide 11 0012 35 Edition AD 57 6 Installation test 6 4 Evaluating the installation test results e Select Control Run and the first part of the report will be printed 4 fuoluatien Tnt tinn test RCS 6070528 TYBIny QuthtiDO LITI fesUNACORMVocalE Mantes veut netaliation jst ACS 070528 TIRINI Owtt za VOTE iii My ula p Piet PICS foi 5 Himi RGS b Fig 6 16 Run procedure At this point close the Procedure Edit window to return to the evaluation diagrams Click on the second chromatogram to make this window active mm nre m ui MIN awn i NT PERM dj j Wi I 070528 TVBIn1 Outlet001 12 Wises sido amano aroro Fig 6 17 Evaluation 2 58 AKTAcrossflow Installation Guide 11 0012 35 Edition AD Installation test 6 In the drop down menu select Procedures Edit New In the Procedure editor choose File Open from file Mon_Valve_TestAD Fpenimation gt Inatallatban test RCS bi Miiat CQutie1 001131 es MACON ecalV Manda Rasi mata fiatind test ACS MO7D528 TYRIN Oun iit Atl uu i Tit Vatvebiioek Test AD tr wifes anoo aos aaoo eira arao anoore SWO mi Fig 6 18 Selection of Mon_Valve_TestAD Select Control Run and the second part of the report will be printed AKTAcrossflow Installation Guide 11 0012 35 Edition AD 59 6 Installation test 6 4 Evaluating the installation test results 60 AKTAcrossflow Installation Guide
6. This guide describes how to install AKTAcrossflow The guide is divided into two parts one describing the installation and one describing how to run the installation test After the installation procedure has been performed your AKTAcrossflow is ready for filtration work For full details of specifications methods maintenance etc refer to the respective User Manuals and Instructions 1 1 Installation Important The installation of KTAcrossflow must be performed by personnel authorized by GE Healthcare 1 2 Associated documentation The following documentation is also included with the KTAcrossflow system The KTAcrossflow Instrument Handbook contains technical information maintenance schedules and instructions for troubleshooting and user maintenance The KTAcrossflow User Manual contains detailed operating instructions The AKTAcrossflow Safety Handbook contains safety information The AKTAcrossflow Method Handbook provides more detailed information on applications AKTAcrossflow Installation Guide 11 0012 35 Edition AD 7 1 Aboutthis installation guide 1 2 Associated documentation The UNICORN control system package includes three manuals e Getting Started e User Reference Manual 2 pcs e Administration and Technical Manual Documentation of the AKTAcrossflow specific Evaluation module includes e User Reference Manual UNICORN 5 1 Evaluation for cross flow e Specific sections in Online
7. 52 5 8 Installing the computer and the MAINS cable 33 5 9 Installingthe Ethernet communication cable 34 5 10 CU 950 configuration i 34 5 11 Connecting Frac 920 optional to AKTACrossfloW 35 5 11 1 Connecting to mains supply sn 55 5 11 2 UNINEt 1 COMMUNICATION 38 6 Installation test 6 1 Preparation of AKTACrOSSIIOW iiii 41 6 1 1 Starting the system 6 2 The installation test 6 2 1 Preparing the installation test iiiiiiiie 43 6 2 2 Level sensor calibration we 44 6 2 3 Runithe iNstallatiO 405 46 6 3 Description of the Installation Test cesecssscccsssssssssseesssssessssssees 50 6 4 Evaluating the installation test results s s 19191119115110115511011101 52 6 4 1 How to start the evaluation procedure manually 56 7 Troubleshooting the Installation Test 8 Installation record AKTAcrossflow Installation Guide 11 0012 35 Edition AD iii 9 Registration form AKTAcrossflow Installation Guide 11 0012 35 Edition AD About this installation guide 1 1 About this installation guide WARNING This instrument is intended for laboratory use only not for clinical or in vitro diagnostic purposes AKTAcrossflow is assembled and fully tested before shipping For safe transportation however some components have been detached and therefore need to be remounted
8. Help 8 AKTAcrossflow Installation Guide 11 0012 35 Edition AD Safety 2 2 Safety e This system is designed for indoor use only e Do not use the system in a dusty atmosphere or close to spraying water e The instrument unit and the PC should be installed and prepared by personnel authorized by GE Healthcare WARNING Heavy object The instrument unit weighs 70 kg Use suitable lifting equipment when moving the instrument Wear safety shoes WARNING The instrument must always be used with the protective earth lead of the power cord correctly grounded to earth at the mains outlet Refer also to AKTAcrossflow Safety Handbook for safety information AKTAcrossflow Installation Guide 11 0012 35 Edition AD 9 2 Safety 10 AKTAcrossflow Installation Guide 11 0012 35 Edition AD Pre requisites 3 3 Pre requisites WARNING AKTAcrossflow must be connected to a grounded mains socket e For lifting instructions see sections 5 3 and 5 4 e To install AKTAcrossflow a working area of about 120 x 60 cm is required e AKTAcrossflow requires 100 to 240 V 50 to 60 Hz electrical supply with safety grounding e A waste flask is needed e The installation test requires the following solutions 3 L of distilled water 1000 ml 1M NaCl with 0 4 acetone and 15 mM phosphoric acid added AKTAcrossflow Installation Guide 11 0012 35 Edition AD 11 3 Pre requisites 12 AKTAcrossflow Installation Guide 11 0012
9. Installation Guide 11 0012 35 Edition AD Installation test 6 5 The window below appears Select a system in the For system drop down menu In the Use frame choose the Wizard button and click Run rahe Mus pt Ang Run Holt Fause 3 Cortina Ena wj sg i Volume crm Acc Time Mock Time Tih low aa Ca Fig 6 5 Selecting the Wizard Read the wizard instructions carefully and follow them exactly select Installation Test as method Click Run 1 Maha mae tha te li sanen a calato buone sini the int 2 Place ad rita arci aen biting me retry ABDUI AVEO PEP VO OL oy a borda cortarng XID ni of desti seer 1 Place cute Ade hom Tarata Page Vota MB Out RADO APND OI rta me bota A Mabie tom Dat ha sesaran kee apan bakoe sharing he best S Mahe noe Poa te aaraa a vot E Mahe noe at teen n moda Tr tekne starting Po Mt Prepare he lumi same sotaan hs tnt of UV pH ard Comt Tavole staen 10 coreano LLOMO 2 Ci Acer 1 tS rl Oro Prenton Acad I pH 2 Mate Lanp Pe rapisci i a batie sa ft hd ho ni metre esco Note Vou md need 13 have a porter rotated a pacer rece goveratet oom tae ne ae ceca Cae setuma Fig 6 6 Selecting method AKTAcrossflow Installation Guide 11 0012 35 Edition AD 47 6 Installation test 6 2 The installation test Co oe oe coe Fig 6 7 Note pad for information on the test 8 Method information is displayed Hold Pause Continue End aoa 7
10. The factory tubing connections are shown in Fig 5 4 Fig 5 4 Tubes connected on delivery Several of the tube connections are plugged when the instrument is delivered Tubes that may have to be connected are those from the transfer valve blocks the permeate valve block the air sensor the retentate valve block and the reservoir The tubing to and from the feed pumps may also have to be reconnected depending on the application Generally connections are made according to the application Refer to the instrument handbook section 2 2 2 Configuration of tubing kits for high and low flow applications KTAcrossflow Installation Guide 11 0012 35 Edition AD Installation of AKTAcrossflow 5 All the tubes are tagged to show where they should be connected The parts to which tubes are connected are shown in Fig 5 5 Transfer pump Permeate pump H sensor Permeate valve P 982 pump P 982 n block Transfer pressure sensor P PCV bnductivity cell UV le cell R PCV Transfer pur valve Feed pump P 984 Reservoir Transfer valve Retentate valve Transfer valve block 1 block block 2 Air sensor Fig 5 5 Connected and ready for operation Be sure to tighten the connectors properly to ensure that no air is sucked into the system and that no liquid leaks out AKTAcrossflow Installation Guide 11 0012 35 Edition AD 29 3 Installation of KTAcrossflow 5 7 Leveling the instrument 30
11. position see figure below Top cover Rear po sition p 32 AKTAcrossflow Installation Guide 11 0012 35 Edition AD Installation of AKTAcrossflow 5 5 8 Installing the computer and the mains cable CAUTION The computer should be installed and used according to the instructions provided by the manufacturer of the computer Unpack and set up the computer according to the manufacturer s instructions Do not switch on AKTAcrossflow Mains cables Fig 5 7 Mains cables Connect one end of the supplied mains cable to the AKTAcrossflow mains inlet and the other end to a mains supply outlet with protective ground Connect one end of the supplied mains cable to the PC mains inlet and the other end to a mains supply outlet with protective ground AKTAcrossflow Installation Guide 11 0012 35 Edition AD 33 5 Installation of AKTAcrossflow 5 9 Installing the Ethernet communication cable 5 9 Installing the Ethernet communication cable CAUTION The mains power to AKTAcrossflow and the PC must be switched OFF before the Ethernet cabling is installed AKTAcrossflow Computer
12. 11 0012 35 Edition AD Troubleshooting the Installation Test 7 7 Troubleshooting the Installation Test The table below describes some of the faults that may arise when performing the installation test Air is drawn in Inlet tubing is not immersed in the water Reservoir was not empty from the start Obstruction in the flow path Transfer valve blocked The transfer purge valve is leaking The level sensor is broken Failed Unit Possible cause Corrective action T VB in 1 to 8 Mismatch between in and out volumes Check all fingertights Make sure that the inlet tube is immersed in water Repeat the test with the reservoir empty from the beginning Check all flow paths and replace if necessary Disassemble the connection block and clean it Contact your GEHC Service representative for replacement of the valve Replace the level sensor Make sure the system is in End mode before starting the system R VB Out 1 to 3 The retentate valve outlet does not provide enough counter pressure Old valve block is used Retentate valve incorrectly No action is required since the old valve block will not provide any back pressure The retentate valve is incorrectly calibrated Wrong UV filter installed calibrated Contact your GEHC Service representative Cond sensor The Cond value did reach its Check calibration Check test solution limit UV sensor The UV did not reach its limit Che
13. 2 35 Edition AD Installation of AKTAcrossflow 5 e Remove the plastic cover from the system e Saveallthe original packing material If the equipment has to be re packed for transportation or otherwise it is important that the system can be safely packed using the original packing material AKTAcrossflow Installation Guide 11 0012 35 Edition AD 17 5 Installation of AKTAcrossflow 5 3 Lifting the instrument 5 3 Lifting the instrument WARNING Heavy object The AKTAcrossflow instrument weighs 70 kg Use suitable lifting equipment to lift the instrument Wear safety shoes WARNING Heavy object The AKTAcrossflow instrument weighs 70 kg A minimum of two people are required to lift the instrument Wear safety shoes After unpacking the instrument lifting equipment is needed to lift the instrument If lifting equipment is not available see instructions in section 5 4 Note The top cover is not mounted at delivery 1 Alifting strap is attached to the instrument at delivery see figure below Lifting strap WARNING The instrument has feet with low friction To prevent the instrument from sliding the workbench surface must not be inclined When moving the instrument on a trolley the instrument must be secured 18 AKTAcrossflow Installation Guide 11 0012 35 Edition AD Installation of AKTAcrossflow 5 2 Lift the instrument onto the workbench or onto a trolley for transportation AKTA
14. 35 Edition AD Installation overview 4 4 Installation overview Unpack AKTACrOSSPIOW scssssscssssssssssesssssesssstnsessssesiassseinnesiunsesiunsesiasessnnsesenesesed 16 e Detach packing Materigl 1111 6cicciciti 16 Uftingithe instrumenti agi iii aaa 18 e Mevingithe instrument adliced Aaa 20 e Levelling the instrumental aiar 30 e Unpackandinstali computet ui aaa 33 CONNECT MAINS power CADIING scsssssssssssssssssssesssssseeceesesseseeceseeseesssssssssssssnsuanssnessees 33 e Connectethernet COMMUNICATION cabling o eeeeeccccssssssssssessssssssssssssssesesessseceesees 34 e Unpack and install Frac 920 optional ccsssessssssssssseesssssseceseeuussssseeseeetansssseeeeeeen 35 e Complete the first two sections of the installation Mecord 63 e Prepare AKTAcrossflow for the installation test nn 41 RUN the installation ESC 46 e Complete the registration TOMA sssini 65 e Complete the final section of the installation record 63 e Store photocopies of all records and forms in the System Logbook AKTAcrossflow Installation Guide 11 0012 35 Edition AD 13 4 Installation overview 14 AKTAcrossflow Installation Guide 11 0012 35 Edition AD Installation of AKTAcrossflow 5 5 Installation of AKTAcrossflow 5 1 Site environment 5 1 1 Room climate Bench space The KTAcrossflow instrument should
15. 5 7 Leveling the instrument After placing the AKTAcrossflow instrument on the laboratory bench the instrument must be leveled The instrument base frame is equipped with four feet One foot is fixed the rear right hand side and the other three are adjustable see figure below Fixed foot Hex socket cap screw Locking nut Fig 5 6 Leveling the instrument The two feet in the front have accessible positions for adjustment see Fig 5 6 AKTAcrossflow Installation Guide 11 0012 35 Edition AD Installation of AKTAcrossflow 5 However to adjust the rear left hand foot the side cover must be removed see figure below Side cover left hand Guide pins long a 3 Rear left hand foot Tip When remounting the left hand side cover first attach the side cover on the lower long guide pins and then attach to the upper short guide pins see figure above To level the instrument see Fig 5 6 1 Loosen the locking nuts 2 Usinga4mm Allen key adjust the height with the hex socket cap screws 3 Check the leveling by using a spirit level on the upper plane 4 Tighten the locking nut AKTAcrossflow Installation Guide 11 0012 35 Edition AD 31 5 Installation of AKTAcrossflow 5 7 Leveling the instrument 5 7 1 Top cover Attach the top cover by first laying it down and then sliding it backwards to the rear
16. Cond Conct setor DE ee ee eee eee eee 0 15 min Transter_Valve_Blocks TABin Logbook A 0 15 min Block Delayp05evn 0 15 min Base Time min 0 20 min End Block 0 20 min Trankte Flow 2000 men 0 50 min Transito Vaive Blocks T VBin pas Fig 6 10 Screen when test has started AKTAcrossflow Installation Guide 11 0012 35 Edition AD 49 6 Installation test 6 3 Description of the Installation Test 50 A description of the test is found in section 6 3 During the test the system will be paused three times with a message indicating that the T VB In1 tubing is to be moved as described below 1 After 25 minutes when the first part of the test is finished Take the T VB In1 tubing out of the water bottle and leave it in the air 2 After another 10 minutes Put the T VB In1 tubing in the test solution 3 After another 5 minutes Put the T VB In1 tubing back in to the water bottle 6 3 Description of the Installation Test First part of the test e Primes all transfer valve inlets with water e Checks for blockage or leakage in all eight transfer valve inlets by pumping a specified amount of water to the reservoir e During this time it also checks the transfer purge valve and the level sensor functionality The system will pause and give a message to end the run if the transfer purge valve is stuck in one position e The first part of the test takes 25 minutes Second part of the test e Fills the r
17. GE Healthcare AKTAcrossflow Installation Guide KTA Important user information All users must read this entire manual to fully understand the safe use of AKTAcrossflow WARNING The WARNING sign highlights instructions that must be followed to avoid personal injury Do not proceed until all stated conditions are clearly understood and met CAUTION The CAUTION sign highlights instructions that must be followed to avoid damage to the product or other equipment Do not proceed until all stated conditions are met and clearly understood Notes Note A Note is used to indicate information that is important for trouble free and optimal use of the product Recycling This symbol indicates that the waste of electrical and electronic equipment must not be disposed as unsorted municipal waste and must be collected separately Please contact EE gn authorized representative of the manufacturer for information concerning the decommissioning of equipment WARNING This is a Class A product In a domestic environment it might cause radio interference in which case the user might be required to take appropriate measures WARNING All repairs should be done by personnel authorized by GE Healthcare Do not open any covers or replace any parts unless specifically stated in the instructions WARNING The computer should be installed and used according to the instructions provided by the manufacturer of
18. Installation test 6 2 The installation test 44 Target values for monitors Cond Minimum amplitude change allowed is 70 0 mS cm 6 2 2 The level sensor must always be calibrated before starting the installation test Level sensor calibration 1 Inthe System Control of UNICORN select File Instant Run Vannitkatwater fhux m42 MnnikaWwater flux007 res Pets a ii ch Volume Acc Tae aa f Pi rar nova gt gt Maeve gt gt Cond gt gt Denar Come actor Fig 6 1 Instant run drop down menu 2 The window below appears Select a system in the For system drop down menu In the Use frame choose the Wizard button and click Run O Systane Content 1 Crunatbow Seviibe Mamat i Wo Aaina Fun0T2 sa Run lot I 6 vinur 1 wou sg x Vostraments Connection Pun Statue Volume orm a Time lock Time n M O CCIE TE uo sn orc 7 ica CE Conn cores Vee AKTAcrossflow Installation Guide 11 0012 35 Edition AD Installation test 6 Fig 6 2 Use Wizard button 3 Inthe Method Wizard dialog select Calibrate level Sensor as method Click Run Manos a The purpose with the callunte level remi method ie 19 anase the assy whan arteria the meri Funcien The sestri mi ng re ceri 10 RVB out ard mabe oe that there i the vetertate hing batoe cafemen Whee the callesnon s trashed he method sd deat the yaen wah mater TB Note The meseron ki raa
19. Stirrer drive motor li cable I 2 Locate the reservoir holder bracket on the instrument unit Reservoir hold er bracket 3 Attach the reservoir holder o o 2 ge pe SH e AKTAcrossflow Installation Guide 11 0012 35 Edition AD 23 5 Installation of AKTAcrossflow 5 5 Installing 4 Tighten the attachment screw Locate the instrument s stirrer drive motor cable and connect the cables Stirrer drive motor connection Attachment screw 5 Insert the connected stirrer drive motor cable into the cable holder under the instrument 10 S Cable holder 24 AKTAcrossflow Installation Guide 11 0012 35 Edition AD Installation of AKTAcrossflow 5 5 5 2 Reservoir 1 Place the reservoir onto the reservoir holder Reservoir Outlet to feed pump manifold Inlet retentate return Reservoir level sensor Bottom end plate Reservoir holder 2 Connect the reservoir level sensor cable to the instrument front panel connector Connection for reservoir level sensor cable Reservoir level sensor Note When using the large reservoir 1100 ml the reservoir level sensor cannot be installed at a right angle towards the front panel as shown in the figure above The reservoir must be rotated to a suitable position for the level sensor AKTAcrossflow Installation Guide 11 0012 35 Edition AD 25 5 Inst
20. allation of AKTAcrossflow 5 5 Installing 3 Connect the inlet and outlet tubing Inlet tubing Retentate return ________ Outlet tubing to feed pump manifold Reservoir level sensor 5 5 3 Bottle holders Attach the bottle holders into the rails on the instrument unit see Fig 5 1 The knob on the bottle holder should fit into the holes in the rail for correct position and the locking device spring loaded ball will keep the holder in place Locking device Fig 5 2 Bottle holder 26 AKTAcrossflow Installation Guide 11 0012 35 Edition AD Installation of AKTAcrossflow 5 5 5 4 Bag holder optional Mount the bag holder to the AKTAcrossflow instrument by attaching the bag holder hooks to the upper rail on the right hand side of the instrument see Fig 5 1 Note The bag holder can only be placed in two positions the front or the rear position on the upper rail see Fig 5 1 Hooks Fig 5 3 Bag holder AKTAcrossflow Installation Guide 11 0012 35 Edition AD 27 g Installation of KTAcrossflow 5 6 Connecting the tubing 28 5 6 Connecting the tubing The tubing connections on the instrument are not complete on delivery All necessary tubing is however included in the delivery and has to be connected before operating the equipment There are a large number of tubes and connection points Refer to section 5 1 7 System flow path in the Instrument Handbook for detailed information
21. be placed on a stable and level laboratory bench Allow space for the monitor beside the instrument KTAcrossflow instrument dimensions 620 x 400 x 650 mm W x D x H Weight instrument unit 70 kg Environment Operating temperature 4 to 40 C 39 to 104 F Relative humidity operation 20 to 95 non condensing Air pressure 75 to 105 kPa up to 2000 m altitude The instrument must not be exposed to direct sunlight Dust in the surrounding atmosphere should be kept to a minimum Power requirements Mains voltage 100 to 240 V 10 50 to 60Hz Auto range Installation category Il Power consumption 900 VA AKTAcrossflow Installation Guide 11 0012 35 Edition AD 15 5 Installation of AKTAcrossflow 5 2 Unpacking the instrument 5 2 Unpacking the instrument Follow the step by step instructions below and fill in the Installation Record as you go along see page 63 Note Some items are packed in separate boxes delivered with the system Note Packing lists are included in the boxes e After removing the cardboard hood and other packing material check the contents against the enclosed packing list Also check all enclosed boxes Store all the boxes and plastic bags in a convenient nearby place Ss e Open the plastic cover from the top and fold down to uncover the system Take care not to damage any tubing or components while doing this 16 AKTAcrossflow Installation Guide 11 001
22. ck filter settings Check test solution Make sure the 280 mm filter is installed and the position of the lamp housing is correct AKTAcrossflow Installation Guide 11 0012 35 Edition AD 61 7 Troubleshooting the Installation Test Failed Unit Possible cause Corrective action pH sensor The pH value did not reach its Check calibration Check test solution limit Sensor not calibrated Calibrate the sensor Sensor broken Replace the sensor R PCV P PCV The R PCV or the P PCV valve Contact your GEHC Service representative did not provide enough counter pressure Pressure sensors The sensor did not detect any pressure Check for leakage Pressure sensor broken Contact your GEHC Service representative for replacement of broken parts R PCV or P PCV broken Contact your GEHC Service representative for replacement of broken parts Pump flow detection The pump did not report any Contact your GEHC Service representative flow Air sensor Air sensor broken Contact your GEHC Service representative Permeate outlets High pressure alarm Disassemble and clean the permeate valve outlets If this does not work call service 62 AKTAcrossflow Installation Guide 11 0012 35 Edition AD Installation record 8 8 Installation record Check Sign Remarks 1 Unpacking e Contents according to packing lists e No visible damage 2 Installation e Reservoir holder and r
23. crossflow Installation Guide 11 0012 35 Edition AD 19 5 Installation of AKTAcrossflow 5 4 Moving the instrument 20 5 4 Moving the instrument WARNING The instrument has a high center of gravity Due to the risk of tipping over do not place the instrument close to the edge of the bench WARNING The instrument has feet with low friction To prevent the instrument from sliding the bench surface must not be inclined WARNING Heavy object The AKTAcrossflow instrument weighs 70 kg A minimum of two people are required to lift the instrument Wear safety shoes CAUTION Bottle holders and the bag holder should not be mounted when lifting and moving the instrument If a smaller movement of the instrument is necessary on the laboratory bench the instrument can be moved by carefully pushing it along the bench surface If a smaller lift of the instrument is necessary i e without using lifting equipment proceed as follows AKTAcrossflow Installation Guide 11 0012 35 Edition AD Installation of AKTAcrossflow 5 1 Usetheslitsinthe bottom frame two slits on each side see figures below to manually lift the instrument Right hand side cover Left hand side cover Slits Slits Note There is no need to remove the side covers when lifting the instrument manually AKTAc
24. ct to the terms and conditions of sale of the company within GE Healthcare which supplies them A copy of these terms and conditions is available on request Contact your local GE Healthcare representative for the most current information GE Healthcare UK Ltd Amersham Place Little Chalfont Buckinghamshire HP7 9NA UK GE Healthcare Bio Sciences Corp 800 Centennial Avenue P O Box 1327 Piscataway NJ 08855 1327 USA GE Healthcare Europe GmbH Munzinger Strasse 5 D 79111 Freiburg Germany GE Healthcare Bio Sciences KK Sanken Bldg 3 25 1 Hyakunincho Shinjuku ku Tokyo 169 0073 Japan 11 0012 35 AD 09 2007
25. eservoir installed e Transfer purge valve Waste 1 extended to waste reservoir e Computer and computer display installed e Ethernet cabling installed e Frac 920 unpacked and installed e UniNet 1 cabling installed e Mains power cabling installed 3 Installation test e Solutions prepared e Tubing to piston seal rinsing system in bottles with 10 mM NaOH in 20 ethanol e AKTAcrossflow prepared e Installation Test run e Registration Form completed e Copy of Registration form stored in System Logbook e Registration form posted to Service Administration e Installation Guide stored in User Manual box for future use AKTAcrossflow Installation Guide 11 0012 35 Edition AD 63 8 Installation record 64 AKTAcrossflow Installation Guide 11 0012 35 Edition AD Registration form 9 9 Registration form Important Warranty registration information Please ensure that this form is completed and returned to Service Administration to register the users equipment under warranty NAME a INStITUTE COMPONY cececsececessecsecsecsecsscsscecseenecuscuececsscsccuecuecussecseenscuseuecessenseneeneenees Address nici icrazioazene zircriianicanicncacinnio sianeaiiaina diana eni dinicanicinani dinanzi Department locations sessecsscsscsscecsecsecsscssecseececsecuscuscnssesseeseeusesscsseecseeneensensseeseessenes POSE COG AEE AE EAEE EE EEE EA Phone Number ssssssesessessereses
26. eservoir with water e Checks that the retentate outlet valves give the right pressure by emptying the reservoir through these outlets e Tests the UV conductivity and pH sensors by pumping the test solution through the permeate line This will indicate that the sensors are working but will not show if they are calibrated correctly e Checks at the same time that the Air sensor is responding by pumping air through transfer valve inlet 1 e Checks that the R PCV and P PCV are working and that there is feed retentate and permeate pressure and flow e Finally it washes out the test solution with water e The second part of the test will take 20 minutes AKTAcrossflow Installation Guide 11 0012 35 Edition AD Installation test 6 Once these tests have been completed the system is filled with water and is ready for use 29001 11 y AUNICORNY Local MAnnikalResult eritication crossflow 11040 1D29001 res DESU SB ofan Ei Teaio cri imam e Wi o r Fig 6 12 Displays the result of the second part of the installation test AKTAcrossflow Installation Guide 11 0012 35 Edition AD 51 6 Installation test 6 4 Evaluating the installation test results 52 6 4 Evaluating the installation test results The system automatically prints the test result when the test is finished The print out includes information about the test and indicates
27. ming the installation test 6 1 Preparation of AKTAcrossflow 6 1 1 Starting the system To start the AKTAcrossflow system 1 Switch on the instrument at the mains power switch located on the rear panel Mains power switch e The Power indicator on the front panel flashes slowly until the internal communication with the CU Control Unit is established Power indicator E ae R e The Power indicator displays a constant green light when the internal AKTAcrossflow Installation Guide 11 0012 35 Edition AD 41 6 Installation test 6 1 Preparation of AKTAcrossflow communication with the CU is established 2 Switch on power to the PC and the monitor 3 Start and log on to UNICORN by double clicking on the icon on the Microsoft Windows desktop ZA ay UNICORN 5 10 4 Enter Username and Password and click OK UNICORN logon User name Loo Password n Note When log on for the first time enter default as user name and password For instructions on how to change user name and password refer to AKTAcrossflow User Manual 5 Inthe System Control module select System Connect to connect UNICORN to the instrument unit System Help Settings Ctrl s Calibrate SRE 4 Take Conn x Connecte System Connect Report Maintena System 1 6 Selec
28. ng the locking screw inside the instrument one turn Locking screw Note The locking screw is intended primarily for EMC grounding 2 Liftup and remove the side cover 38 AKTAcrossflow Installation Guide 11 0012 35 Edition AD Installation of AKTAcrossflow 5 3 Locate and remove the UnitNet 1 termination plug and connect one end of the UniNet 1 cable included with the fraction collector instead UniNet 1 termination plug Tip Save the termination plug in for example the accessory box 4 Connect the other end of the UniNet 1 cable to Frac 920 see Fig 5 9 and Fig 5 10 AKTAcrossflow Fraction collector Computer UniNet 1 Ethernet Fig 5 10 Communication cabling including a fraction collector AKTAcrossflow Installation Guide 11 0012 35 Edition AD 39 5 Installation of AKTAcrossflow 5 11 Connecting Frac 920 optional to AKTAcrossflow 40 AKTAcrossflow Installation Guide 11 0012 35 Edition AD Installation test 6 6 Installation test The installation test is designed to check the function of all pumps and valves and the monitoring system of AKTAcrossflow The installation test can be used at any time to check the condition of the system IMPORTANT The level sensor must always be calibrated before perfor
29. ossflow Installation Guide 11 0012 35 Edition AD 55 6 Installation test 6 4 Evaluating the installation test results 56 If any parts of the test should fail refer to the troubleshooting table in this document or consult the instrument handbook for more detailed troubleshooting guidelines A failure may sometimes be caused by something as basic as a leaking connection that has not been tightened properly Always check the basics before heading into advanced fault analysis 6 4 1 Howto start the evaluation procedure manually If for some reason no printout was generated even though the evaluation was performed correctly then proceed as follows e Open the result file in the UNICORN Evaluation window and click on the first chromatogram to make it active v WNICORNLocallFillAnnikalResult lentatlation test RCS b07052 Fie Edt View Integrate Operations Procedures Window Me Oc Se OHAN BB installation to Racart ant Fies Find E Recent Rest w D installation test RE a tata at amp T installation test ERTI lit iii i bb d h Fig 6 13 Evaluation AKTAcrossflow Installation Guide 11 0012 35 Edition AD Installation test 6 e Select Procedures Edit New Fig 6 14 Select new procedure In the Procedure Edit window Select File Open from file Trf_ValveBlockTest_AD Fe LR See mtego Orani riran Winter Heb DEA Ss ofan Fig 6 15 Procedure editor
30. r CURVE AMPLITUDE CHANGE 4 EST RESULT Feed pressure sensor OK AKTAcrossflow Installation Guide 11 0012 35 Edition AD Installation test 6 The change in amplitude was 1 89000 bar Minimum amplitude change allowed is 0 20000 bar CURVE AMPLITUDE CHANGE TEST RESULT Retentate pressure sensor OK The change in amplitude was 1 59000 bar Minimum amplitude change allowed is 0 20000 bar CURVE AMPLITUDE CHANGE TEST RESULT Permeate pressure sensor OK The change in amplitude was 1 68000 bar Minimum amplitude change allowed is 0 20000 bar CURVE AMPLITUDE CHANGE TEST RESULT Feed flow detected OK The change in amplitude was 350 00000 ml min Minimum amplitude change allowed is 1 00000 ml min CURVE AMPLITUDE CHANGE TEST RESULT Permeate flow detected OK The change in amplitude was 200 00000 ml min Minimum amplitude change allowed is 1 00000 ml min CURVE AMPLITUDE CHANGE TEST RESULT Transfer flow detected OK The change in amplitude was 200 00000 ml min Minimum amplitude change allowed is 1 00000 ml min Note If the system is equipped with an old type of retentate valve block the test result of R VB Out1 3 will be Failed The installation test is now complete and the unit can be used in normal operation AKTAcr
31. rossflow Installation Guide 11 0012 35 Edition AD 21 5 Installation of AKTAcrossflow 5 5 Installing 5 5 Installing Begin by creating a clean and dry working area of 120 x 60 cm that allows easy access WARNING Select a place for AKTAcrossflow where it is easy to access the mains power switch on the rear panel of the instrument WARNING Do not block the rear panel of the system The mains power switch must always be easy to access Then follow the step by step instructions below and fill in the installation record as you go along see page 63 For safe transportation the following components have been detached and thus need to be remounted e Reservoir holder e Reservoir e Bottle holders e Bag holder optional Bag holder rear position Upper rail hidden Bottle holders ip KC IF Bottle holders lf ls fe S al ley an F ga i KE eee I Reservoir A Reservoir holder 22 Fig 5 1 Location of components to be remounted AKTAcrossflow Installation Guide 11 0012 35 Edition AD Installation of AKTAcrossflow 5 5 5 1 Reservoir holder Install the reservoir holder as follows 1 Unpack the reservoir holder Reservoir holder
32. sessseesseeessressseessse Fax NUMDEL ssisiserissssesssssssisssosssssssnsrasi ENDA USETS siii E MG l cicci Date of Installation rrrrrrreeeerere Quote NO sccsrrrsecrezereee Customer Order NO scsrrreseeeeee INVOICE NO rsrrsrcrrerezenne Support Agreement purchased with the instrument Y N If VESIgive det llSz uani aa Installer NAM Liu Note Fill in serial numbers over leaf AKTAcrossflow Installation Guide 11 0012 35 Edition AD 65 9 Registration form 66 AKTAcrossflow Installation Guide 11 0012 35 Edition AD For contact information for your local office please visit www gelifesciences com contact GE Healthcare Bio Sciences AB Bj rkgatan 30 751 84 Uppsala Sweden www gelifesciences com imagination at work GE imagination at work and GE monogram are trademarks of General Electric Company Drop Design UNICORN AKTAcrossflow and AKTA are trademarks of GE Healthcare companies UNICORN Any use of this software is subject to GE Healthcare Standard Software End User License Agreement for Life Sciences Software Products A copy of this Standard Software End User License Agreement is available on request All third party trademarks are the property of their respective owners 2005 2007 General Electric Company All rights reserved First published February 2005 All goods and services are sold subje
33. t be open ing Woe seni cito lo ensure a goed rendi 110 min Transtor Valve Blocks T 1 40 min Transtar Valve Blocks T 1 70 min Transter_Veive_Biocks T 2 00 min Transtar Vaive Blocks TS 2 30 min Transtor_Velve_Blocks TA 2 35 min End 2007 05 25 095340 Coral European SSanderd a face emi CIS Ea oini y Arda Fig 6 3 Method Calibrate level sensor The level sensor will now be calibrated The method takes about five minutes KTAcrossflow Installation Guide 11 0012 35 Edition AD 45 6 Installation test 6 2 The installation test 6 2 3 Run the installation test IMPORTANT The level sensor must always be calibrated before running the installation test See 6 2 2 The reservoir must be empty before starting the installation test Make sure all finger tight connectors are tightened Note Open the reservoir lid when performing the Installation Test The Installation Test will prompt you to do this You will also be instructed on how and where to place the waste lines 1 In order to get a reasonable view of the Installation Test in the system control adjust the visible curves to a minimum and open View Properties in the toolbar 2 Deselect all curves and mark FeedFlow TransferFlow FeedPress and ResVol 3 Goto Y axis and set the selected curves to Auto scale RN select File Instant Run kt Result v Annika water flux002 re5 Fig 6 4 Drop down menu 46 AKTAcrossflow
34. t the appropriate system name and click OK 42 AKTAcrossflow Installation Guide 11 0012 35 Edition AD Installation test 6 When the communication between UNICORN and the instrument unit is established e There is a constant green light on the Power indicator on the front panel e The green Run indicator in the status bar in UNICORN is lit e The Connection box shows Yes Palin 1 lu u Run al iia dnl A T a Run Data Instruments Connection Run Status Acc Volume Block Volume Ace Time Block Time CC 6 2 The installation test 6 2 1 Preparing the installation test Prior to running the installation test follow the instructions below 1 Place all inlet and outlet tubing not including R VB Out1 R VB Out2 and P VB Out1 into a bottle containing 3 liters of distilled water Place outlet tubing from the transfer purge valve R VB Out1 R VB Out2 and P VB Out1 into a 3 L waste bottle Sample solution Prepare the following sample solution for test of UV pH and Cond Sample solution 1000 ml containing NaCl 1 00 M 58 44 g Acetone 0 4 4 ml Phosphoric acid 15 mM 1 0 ml H3PO Note Keep the sample solution in a bottle with tight lid to avoid acetone evaporation Target values for monitors UV Minimum amplitude change allowed is 85 0 mAU pH Minimum amplitude change allowed is 2 0 AKTAcrossflow Installation Guide 11 0012 35 Edition AD 43 6
35. the computer WARNING The mains power switch or other disconnect device must always be easy to access CE certification This product complies with the European directives by fulfilling corresponding standards A copy of the Declaration of Conformity is available on request The CE logo and corresponding declaration of conformity is valid for the instrument when it is used asa stand alone unit or connected to other CE marked GE Healthcare instruments or connected to other products recommended or described in this manual and used in the same state as it was delivered from GE Healthcare except for alterations described in this manual Note The Declaration of conformity is valid only for systems that are marked with the CE logo CE Contents 1 Aboutthisinstallation guide 11 Installation Rca 7 1 2 Associated documentation uu 7 2 Safety 3 Pre requisites 4 Installation overview 5 Installation of AKTAcrossflow 5 1 Site environment sk 5 1 1 ROOM ClIMAte dS 5 2 Unpacking the instrument ccsccssccsssscsssssssssssessessccssssssssssssusessessee 16 53 Ufingihne strumenti lia 5 4 Moving the instrument 55 INStAlling 5 5 1 Reservoir holder du 5 5 2 RESEO ett ae a e ced a eR es 5 5 3 BOttle Rolde S a tarini 5 5 4 Bag holder optional 5 6 Connecting the tubing 5 7 Leveling the instrument i 30 5 7 1 Top cover we
36. whether the test results are satisfactory or not Below is a typical example of a test result TEST INFORMATION Installation test result System name Xflow_28239 UNICORN version UNICORN 5 11 Build 407 Time 2007 06 04 13 00 38 Central European Daylight Time User John Smith Result file v UNICORN Local Fil John Smith Result Installation test RC5 0 LOGBOOK EVENT TEST RESULT Level sensor functionality OK LOGBOOK EVENT TEST RESULT Transfer purge valve OK LOGBOOK EVENT TEST RESULT T VB In8 OK LOGBOOK EVENT TEST RESULT T VB In7 OK LOGBOOK EVENT TEST RESULT T VB In6 OK LOGBOOK EVENT TEST RESULT AKTAcrossflow Installation Guide 11 0012 35 Edition AD Installation test 6 T VB In5 OK LOGBOOK EVENT TEST RESULT T VB In4 OK LOGBOOK EVENT TEST RESULT T VB In3 OK LOGBOOK EVENT TEST RESULT T VB In2 OK LOGBOOK EVENT TEST RESULT T VB Inl OK EST INFORMATION Installation test result Xflow_28239 System name UNICORN version Time User John Smi Result file Installation Vv th UNICORN Local Fil John Smith Result test RC5 0 UNICORN 5 11 Build 407 Time 2007 06 04 14 41 48 Central European Daylight
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