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1. 0 asessessesssseeeeee emen Korean amp Portuguese BBSODEIDN adtassientDEo DADA MD pud SR QUEE Oty U TT Datalogging Manual timed selectable every 3 to 86400 sec 24hours S Hrl e Nro 03 C 0 5 TDS Calibration Offset in 0 1 increments Offset range 5 C 9 F Range 222os2seseseeee 0 00ppm to 500ppt TDS factor 1 00 lon Mode MBSORMID 5 esticncthceaceraancacates cations 0 01 0 1ppm 0 001 0 01 0 1ppt ee dunonactonit aono 0 001 to 19999 2000mV PON dusneitGaNw Muiattidn ia MD ANN dE 1 96 full scale HBSOUNUN 5 undici iss 0 001 0 01 0 1 1 automatic LAELIUS eg see eat Mui UC E Up to 5 PDT rm ppm mg L molar i eee 0 400 to 1 000 Cat No Description 12820653 XL600 Meter Includes meter electrode arm RS 232 amp USB cables 100 240V power supply and manual 12810653 X600 Kit Includes meter TRIS compatible accuTupH pH electrode Cat No 11550174 ATC probe Cat No 10236064 conductivity temp probe Cat No 11550164 electrode arm RS 232 amp USB cables 100 240V power supply and manual 12830653 XL600 Deluxe Kit Includes meter self stirring BOD probe Cat No 11530184 TRIS compatible accuTupH pH electrode Cat No 11550174 ATC probe Cat No 10236064 conductivity temp probe Cat No 11550164 electrode arm RS 232 amp USB cables 100 240V power supply and manual J 11550174 10236064 11550164 11510154 11510134 115301
2. Aduiensusenistuctenicenatuepa dtestuspnsud conn 0 01 0 1 1 10 100 1000 RN NIST 1 678 4 010 6 865 9 184 12 460 H ROES 020 2 220 200 2000 Mec E DIN 1 090 3 060 4 650 6 790 9 230 12 750 E OX de 1 OU 1000 10000 uM US FSCI 1 000 3 000 6 000 8 000 10 000 13 000 MPIU ERN LBULRLUS CREUSE LIU rore CUSTOM Any 2 5 values 21 0pH unit apart Temperature Mode Slope DISOEN ee ee de neta Yes up to 5 different slopes with offset ro ecc i 0 1 0 01 0 001 pH Temp Compensation Automatic or manual 0 to 100 C 32 to 212 F ucro c 0 002pH 1LSD mV Mode Calibration sissie Offset in 0 1 increments offset range 5 C 9 F s M ectocn ecco see ees 2 000 to 20 000pH Output C NG icc tbc aera RS 232 phono plug mini B USB stirrer Pesonn M teeta ee OTR 0 1 0 01 0 001pH Language Selection English French Spanish German Italian Chinese Korean amp POON ror LOOR ge Fb 5 5 oc reece Teer VOR ene TO CeO re SY eT Ty Portuguese Offer Adjustment nnn ene ee ene ey nen ee ee aas Up to 150mV Memor uueteuntentunddetatutzdat cuia ndum 500 data sets viewable lon Mode Datalogging J Manual timed selectable every 3 to 3600 seconds Range ri tsa Bisa iniu deitas 0 001 to 19999 x2000mV 0 0 0 0 00000000 essent Printer or CSV format Resolution 5 5 besagt qn eise a aai
3. Frequently asked questions FAQ s his brochure features some of the most frequently asked questions about electrochemistry and pH measurement as received by our Life Science and Chemical Specialists together with the answers they provided However if you are unable to find the answer to your question are stuck and need help or are simply confused and unsure of which product best suits your research needs the Product Support Team are here and ready to respond to your enquiries Have a Confused Stuck question need help Contact our Product Support Advisors 4p lel O 1509 555 888 Tel 01 885 5854 b Email fisheruk productsupportQthermofisher com Email fsie sales thermofisher com Laboratory Reagents Handbook For a fuller range of Fisher Chemical and Fisher Bioreagents please refer to our Laboratory Reagents handbook This handbook features For the analytical chemist e Over 4400 Fisher Chemical products dedicated to many analytical applications including Optima LC MS grade solvents and high purity acids for Trace Elemental analysis Colour coded applications Physical amp chemical data Fisher Hazard packaging and storage information Chemical Detailed specifications For the life scientist P e A dedicated section relating to four key application areas Amiha Protein Chemistry Handbook Molecular Biology Cell Biology Core Bioreagents Fisher BioReagents To order your copy visit www eu fish
4. General field purpose General laboratory purpose General laboratory purpose Laboratory purpose Laboratory purpose Low inonic strength Nonaqueous viscous samples Small samples Small samples Food and drink Environmental samples Environmental samples Environmental samples Environmental samples des where membrane breakage is a problem The rugged pH bulb is protected by unique Tuff Tip shape making this electrode shock proof 6 ions mm 11758452 Cable Connector dm BNC im DN E Dimensions mm 412x120 12x 120 2 08 09 2014 16 16 12 pH Buffers 20 STANDARD BUFFER SOLUTIONS FOR pH METER CALIBRATION Fisher Chemical Next to temperature and mass pH is the third most common laboratory measurement and buffer solutions such as phosphate buffered saline PBS are frequently used as a means of keeping pH at a nearly constant value in a wide variety of chemicals and biochemical applications For example many enzymes only work under very precise conditions so it is very important that the buffer maintains the correct pH if the pH moves outside of a narrow range the enzyme may stop working and denature Industrially buffers are used in fermentation processes and in setting the correct conditions for dyes used in colouring fabrics For such very precise work it is important that pH meters are calibrated before each measurement Standard buffer solutions of k
5. 11768452 ORP Electrodes Cat No Electrolyte 11768452 Gel 11778452 Gel 11758452 Gel Body Material Plastic Plastic Plastic Electrode Accessories Cat No Desctiption 11746348 11736348 Connector cable S7 to BNC 1m Connector cable S7 to DIN 1m IE UK EN 2014 200 10187 FOCUS ph electrochem master indd 27 11786338 11726358 Body Material Plastic Plastic Plastic Glass Glass Glass Glass Glass Glass Glass Plastic Plastic Plastic Plastic 11769798 pH Range to 13 Oto 13 0to 14 Oto 13 to 13 Oto 14 0to 14 Oto 13 to 13 1t011 Oto 14 Oto 14 Dto 13 Oto 13 Temperature Range C to 80 Oto 80 Oto 80 Oto 80 Oto 80 Oto 80 Oto 80 Oto 80 to 80 to 70 bto 100 5 to 100 5to 100 510 100 11778452 pH Range 81500 mV 1500 mV 1500 mV www eu fishersci com i 11709818 Dimensions mm 2209178 12x 120 SP 12x 120 Sip 12x160 12 x 120 xII CE 6x9 Eo 12x120 P 12x120 Temperature _ Range C 01080 0 to 80 0 to 80 im dm dm Cable 11755638 11765638 11775638 11785638 BNC DIN a BNC DIN 7 87 BNC DIN 7 1 Dimens 12x 120 12x 120 12x 120 BNC DIN BNC DIN pH Electrodes 11736209 Sample Connector Applicaton Type General field purpose General field purpose
6. Cat No Description 10104823 Buffer standard solution pH3 00 phthalate stabilised with 10ppm mercury II chloride traceable to NIST 10111430 Buffer standard solution pH3 00 phthalate stabilised with 10ppm mercury Il chloride traceable to NIST 10665492 Buffer standard solution pH3 00 phthalate stabilised with 10ppm mercury Il chloride traceable to NIST Fisher OH4 Buffers Chemical Cat No _ Description 10734751 Buffer tablets pH4 00 phthalate makes 100mL of solution per tablet 10545151 Buffer standard solution pH4 00 phthalate stabilised with 10ppm mercury Il chloride traceable to NIST 10675492 Buffer standard solution pH4 00 phthalate stabilised with 10ppm mercury II chloride traceable to NIST 10030190 Buffer standard solution pH4 00 phthalate stabilised with 10ppm mercury II chloride traceable to NIST 10040190 Buffer standard solution pH4 00 phthalate stabilised with 10ppm mercury II chloride traceable to NIST 10305950 Buffer standard solution pH4 00 phthalate stabilised with 10ppm mercury II chloride traceable to NIST 10508050 Buffer concentrated solution pH4 00 phthalate stabilised with 10ppm mercury II chloride each ampoule makes 500mL 10234390 Buffer standard solution BS pH4 00 phthalate stabilised with 10ppm mercuric chloride BS 1647 traceable to NIST 11413843 Buffer concentrated solution BS pH4 00 phthalate stabilised with 10ppm mercuric chloride one ampoule makes 500
7. Ethylenediaminetetraacetic acid trisodium salt concentrated solution 0 2N 0 1 M ampoule Ethylenediaminetetraacetic acid disodium salt solution 0 02N ready to use Ethylenediaminetetraacetic acid disodium salt solution 0 02N ready to use Ethylenediaminetetraacetic acid disodium salt solution 0 2N ready to use Ethylenediaminetetraacetic acid disodium salt solution 0 2N ready to use Ethylenediaminetetraacetic acid disodium salt solution 0 2N ready to use Ethylenediaminetetraacetic acid disodium salt concentrated solution 0 02N ampoule Hydrochloric acid pure solution 5M bench reagent ready to use Hydrochloric acid solution 0 02M 0 02N ready to use Hydrochloric acid solution 0 02M 0 02N ready to use Hydrochloric acid solution 0 1M 0 1N ready to use Hydrochloric acid solution 0 1M 0 1N ready to use Hydrochloric acid solution 0 1M 0 1N ready to use Hydrochloric acid solution 0 1M 0 1N ready to use Hydrochloric acid solution 0 2M 0 2N ready to use Hydrochloric acid solution 0 2M 0 2N ready to use Hydrochloric acid solution 0 5M 0 5N ready to use Hydrochloric acid solution 0 5M 0 5N ready to use Hydrochloric acid solution 1M 1N ready to use Hydrochloric acid solution 1M 1N ready to use Hydrochloric acid solution 1M 1N ready to use Hydrochloric acid solution 1M 1N ready to use Hydrochloric acid solution 2M 2N ready to use Hydrochloric acid
8. 08 09 2014 16 22 39 Whatever your application Fisherbrand has a solution for you UK IE EN 2014 200 10187 TESDIKESENE2 0192007901872 EO GU S ph electrochememasterdindd 68 08 09 2014 16 22 54
9. 15121499 Nylon 2 02 No 15131499 Nylon 25 0 45 No 15161499 IPIE i 0 2 No 15171499 PTFE 13 0 45 No 15141499 PTFE 25 02 No 15151499 PTFE 25 0 45 No 15181499 PVDF B 02 Yes 15191499 PVDF 33 0 45 Yes 15181489 RE 25 02 No 15101499 RC 2 0 45 No Pack qty 50 50 50 50 100 100 50 50 50 50 t 50 www eu fishersci com IE UK EN 2014 200 10187 FOCUS ph electrochem master indd 57 po cee eee 9 08 09 2014 16 21 55 Spatula balance Iraceable M e Measuring range of Og to 300g e Holds 32mL 1 1 fluid ounces e Readability 0 1g e Accuracy 0 2g e Chemical corrosion resistant ABS plastic construction Cat No Description Pack qty 11744416 SpatulaBalance V 1 Stirrers magnetic unheated mini e Powerful enough to stir 1L of liquid at up to 2 000rpm e Manufactured from a tough polypropylene with a clear chemically resistant polycarbonate top e With instructions stir bar and power adapter Technical Specification VBSSB DAP Eann aen uci utendi Sait a en DE GEI La E EE 1 max b IB Si MD ERR EC ee EA eee eee eee E 85 max sc cu ce T E ET rr SM 350 to 2 000 Dimensions w x d X h MM RRTORRR 143 x 66 x 143 DIE Ub anu ccu M E IMEEM D RE D M M LU M EDU 400 a SUED EIA EAE EET A E A OOR E T E errr terre ttt aera 230V 50Hz Cat No Description Pack qty 11577493 Basic mini stirrer Fisherbrand i 11597493 Basic mini stirrer Caffeine 1 11507503 Basic mini stirrer Alchemist i 11517503
10. 500 5000 NIST 1 678 4 010 6 865 9 184 12 460 Conductivity eee DIN 1 090 3 060 4 650 6 790 9 230 12 750 Range rre UO TOES BB S VOCE FSCI 1 000 3 000 6 000 8 000 10 000 13 000 Resolution onere O DET UOT J U0 0 TRIS CUSTOM Any 2 5 values 21 0pH unit apart Accuracy X Na LE IIS SW DGI ec Yes with offset GAL POI sinansa Automatic 4 points maximum 1 per range Multiple Slope Display cece Yes up to 5 different slopes 11 2222222 Manual 5 points maximum 1 per range Temp Compensation Automatic or manual 0 to 100 C 32 to 212 F Gell Constant eem 0 010 to 10 000 Temp Range Meter 0 0 to 100 0 C 32 0 to 212 0 F POI A m 2 or 4 cell with ATC 1 106 Mmm BNC ATC Coefficient Per C Linear amp pure adjustable 0 000 to 10 000 96 mV Mode Nonna 15 0 to 30 0 C 59 0 to 86 0 F 1n O 2000 0mV Compensation Automatic with supplied cell or manual LMV D A Temm 2000 0mV Temp GOmpEIsallUli saos tr oret 0 0 to 100 C 32 0 to 212 0 F licia M MM e 1 MM ZZ LL LL L LLL 0 0 to 80 C 32 0 to 176 0 F with supplied cell PODUFGDY anmnuieeiisikex 0 2mV or 0 05 whichever is greater Output ee RJ45 RS232 USB A mini B USB two stirrer ports Uifset Adjustment uiuere be reote i ori e eee Etre Up to 150mV Language selection English French Spanish German Italian Chinese Temperature Mode
11. Auto ranging for conductivity and TDS measurements offers fast response over the entire range Adjust temperature coefficient from 0 to 10 per C or F for accurate compensation in almost any solution The AP75 meter offers a real time clock expanded memory function and selectable cell constant The AP85 meter measures pH conductivity and TDS plus temperature AP85 also features five point pH calibration with ATC and auto buffer recognition The AP100 series meters are small enough for a shirt pocket yet smart enough for any laboratory The kits include an unbreakable pH temperature electrode that is refillable for a fast response and the longest life The AP125 accepts any ion selective electrode ISE with a BNC connection for direct ion concentration measurement from 0 001 to 99 999ppm In this section discover why this range of Fisherbrand accumet portable meters are perfect for the researcher on the go To view the instruction manuals for the following range of accumet benchtop meters visit www eu fishersci com fisherbrand Selection Guide for Portable Meters Find the right accumet portable meter for your particular application using the selection guide below Conductivity Dissolved Oxygen ISE ATC Date Time Data Point Storage IE UK EN 2014 200 10187 FOCUS ph electrochem master indd 16 AP74 AP84 18 18 e e e e e e 50 www eu fishersci com v wer AP125 200 08 09 2014 16 15 10 P
12. _ of Buffers at _ Various _ Temperatures 1 68 4 01 6 86 7 00 9 18 10 01 12 46 Q Can mix and match meters and electrodes from different manufacturers 1 67 4 00 6 98 71 946 10 32 mou ET dn 4 00 6 95 7 08 940 1025 PR 10 C ay 4 00 Be 7 06 933 10 18 12 67 20 C 167 4 00 687 101 923 10 06 1252 30 C 1 68 402 6 85 6 98 914 9 97 1236 40 C 1 69 4 04 684 697 9 07 9 89 ea 50 C i 406 6 83 6 97 901 983 11 96 60C Bez 4 09 6 84 6 97 8 96 979 S 70 C 1 74 413 2555 6 99 8 92 9 78 1147 Sc ZEE 4 16 6 86 7 03 8 89 978 11 19 90 C 179 421 6 88 7 08 8 85 9 80 10 89 A This is generally not a problem The vast majority of manufacturers nowadays use a BNC connection between the electrode and the meter for standard pH electrodes This can however cause an issue when using an ATC probe as these connectors are not standardised and are manufacturer specific Q How regularly should clean my electrode A As regularly as possible Cleaning and maintaining it will help prolong the electrodes life It is worth noting that you must be careful not to leave an electrode soaking in aggressive cleaning solutions once clean This could end up damaging the electrode Key points to remember 42 e Never re use buffers e Never polish the bulb e Never store the electrode dry or
13. e Speed rpm max 6 000 e Dimensions W x D x H mm 153 x 128 x 104 e UV resistant plastic e Includes two interchangeable rotors six place standard rotor and an eight place strip rotor Cat No Description Pack qty 13406188 Mini centrifuge Midi centrifuge e Speed rpm 500 to 12 500 e Dimensions W x D x Hj mm 203 x 171 x 114 e Tool free quick change rotor system Supplied with two rotors twelve place standard rotor with lid for 1 5 2 0mL tubes eight place strip rotor for four 0 2mL tube strips or 32 single tubes twelve 0 2mL amp 0 5mL adapters for customised use Cat No Description Pack qty 12972041 Midi centrifuge m Centrifuge tubes O 6mL to 2 0mL Locking lid microcentrifuge tubes with polypropylene Snap Cap e Convenient pierceable locking lids snap into place with one handed operation e Certified free of DNase RNase ATP and pyrogen e Autoclavable e Withstand freezing 80 C Cat No Capacity mL RCF x g max Pack qty 11706467 as 24 000 500 11313633 20 24 000 500 Siliconised low retention microcentrifuge tubes e Snap Cap lid provides a secure seal even over extended periods Cat No Capacity mL Height x diameter mm Pack qty 11323653 0 6 30 x 10 500 11986955 15 40x 13 250 Microtube 0 6 to 2mL graduated e Flap cap e Withstand up to 30 000xg e Free of DNase RNase Cat No Capacity mL Height x diameter mm Pack qty 11916955 0 6 TENES qe 500 11
14. 10 3 10 Borax 9 3 9 9 4 Lime soda softening 8 4 Baking soda Saltwater aquarium 8 0 8 8 0 Seawater Blood 7 5 7 4 Swimming pool water Fresh water aquarium 7 1 7 0 Distilled water Milk 6 7 Corn 6 2 6 6 3 Brewing process 5 8 Nickel plating Boric acid 5 0 S 5 0 Food processing 4 4 3 Orange juice Pickle processing 3 5 3 2 Photo engraving Vinegar 2 9 7 2 3 Lemon juice Battery acid 0 3 0 pH is used as a convenient way to compare the relative acidity or alkalinity of a solution at a given temperature The term pH itself is derived from a combination of p for the word power and H for the symbol of the element hydrogen Together the meaning is the power of hydrogen Therefore the pH is the degree of acidity or alkalinity of a solution based on the hydrogen ion activity represented by the equation pH log H Stated mathematically pH is the negative logarithmic value of the hydrogen ion As it is based on a log scale each pH unit represents a factor of 10 so a solution with a pH of 5 is 100 times more acidic than pH of 7 pH is measured on a scale of 1 14 1 being very acidic 14 being very alkaline and 7 neutral A pH of 7 describes a neutral solution because the activities of hydrogen and hydroxide ions are equal When the pH is below 7 the solution is described as acidic because the activity of hydrogen ion is greater than that of hydroxide ion and as the hydrogen ion activity incre
15. 100 81158 o MM LL NEN RES Korean amp Portuguese REESE nsmesdihixdesbistc iuam qd MES 0 1 C 0 1 F Calibration O O aereoa aa ciem ne 0 2 C 0 3 F CatNo Description 12880643 XL500 Meter Includes meter electrode arm RS 232 amp USB cables 100 240V power supply and manual XL500 Kit Includes meter TRIS compatible accuTupH pH electrode Cat No 11550174 ATC probe Cat No 10236064 Conductivity Temp probe 12800653 Cat No 11550164 electrode arm RS 232 amp USB cables 100 240 V power supply and manual Www eu fishersci com IE UK EN 2014 200 10187 FOCUS ph electrochem master indd 14 08 09 2014 16 14 58 Benchtop Meters pH Meter XL 600 A complete lab in one instrument Technical Specification ROGUEBU aedon iiini 0 5 full scale monovalent ion O ES 1 full scale divalent ion r1 Np 2 000 to 20 000pH 2 jc Nnm 0 001 0 01 0 1 1 10 100 Resolution 0 1 0 01 0 001 selectable 2 AM eeeee 0 01 0 1 1 10 100 1000 ACUO anusstestuseutemiias nn uei SELUOSMUDOZSIESD O O geniaicieinminu setis 0 02 0 2 2 20 200 2000 PaO Sacenaituniut iutuieielii i UnTODDISSBEOK o custam aeudnieitieeetnsienetesteiutiainn 0 1 1 10 100 1000 10000 Butor SES innssssenien U5A 2DDD ATG SS IOUOTS TZDDU 8 estacassuuistieaitsuetidiatintis 0 05 0 5 5 50
16. 21 Conductivity Dissolved Oxygen and Temperature Probes 21 Cables and Other General ACC6eSSOrIeS sssssssee ener nennen tenent nn 27 pH ELECTRODES tt u 23 pH Electrode Theory 1 5 ssec 23 e Relationship Between pH and Temperature sss 23 e Electrode Selection sss teet 24 dossier d NT RETE 24 x ods are PRETI 24 d Ucereditsu ii54 fm KE 24 Single or Double Junction TRIS comnqpatible ssseeennnnnnn nnns 21 pH Electrode Features 1 1 sss teet tn 25 e pH Electrode Selection Guide 1 21 701 0st 26 pH Electrode Connectors stet tetti 26 DH ci etgelei cR a eee eer ee ee eee a eee ee ee 27 b tizindimii ielidele ED EU 27 e Electrode Accessories 1 sssssstt tnter n STANDARD BUFFER SOLUTIONS FOR pH METER CALIBRATION 28 e Colour Coded Buffer Solutions sss 29 Wwww eu fishersci com 08 09 2014 16 14 04 IE UK EN 2014 200 10187 FOCUS ph electrochem master indd 5 pH Accessories gba 0a 1 0 L S NI RTT 31 DEE gcc AINA ETE 31 DH Indicator uz 9 NES Ie c 31 DH Macato ECRANS momo 3 DISPORRE HDD asses a satus E Ine SER Sabe CRUCEM Ie UNE 31 TECHNICAL RESOURCES ttt 32 DH Electrode Preparation 1 1 1 sss 32 e pH Electrode Calibration 1 1 ssec 33 Calibration Recommendations nnne
17. 96 11933416 0 1 to 10 Bulk Clear E 10yL extended length micropoint tip graduated at 2 5pL 11913466 0 1 to 10 Filtered sterile Clear 96 11983416 0 1 to 10 Bulk Clear 20pL universal bevelled tip 11943466 2 to 20 Filtered sterile Clear 96 100pL universal bevelled tip 11953466 10 to 100 Filtered sterile Clear 96 200pL universal bevelled tip 10124314 1 to 200 Bulk Yellow 200pL universal micropoint tip graduated at 10pL 50pL and 100pL 11963466 20 to 200 Filtered sterile Clear 96 1 000pL universal micropoint tip graduated at 100pL 200pL 500pL and 1 000pL 11973466 10 to 1 000 Filtered sterile Clear 96 1250yL universal micropoint tip graduated at 100pL 200pL 500pL and 1 000pL 10778535 100 to 1 250 Racked sterile Clear 96 Micropoint tip Eppendorf style 11903426 0 1 to 20 Bulk Clear Reagent reservoirs Cat No Capacity mL Material Sterile 11908495 50 PVC No 11988485 50 PS Yes 11978485 50 iS aus 11998485 100 PS Yes Syringe filters NEW Pack qty 960 1 000 960 1 000 960 960 1 000 960 960 960 1 000 Pack qty 100 80 200 200 e Available in a variety of membrane types polyethersulfone PES nylon polytetrafluoroethylene PTFE polyvinylidene fluoride PVDF and regenerated cellulose RC e 3 different sizes 13mm 25mm and 33mm Cat No Membrane Diameter Pore size Sterile mm um 15206869 _PES 3 0 2 Yes 15216869 PES 33 0 45 Yes
18. A To ensure accurate and reliable readings we always recommend calibrating in three pH buffers normally pH 4 7 and 10 However depending on the accuracy you actually require this can be done at as few as two pH points e g 4 and 7 or 7 and 10 or as many as five points on the Fisherbrand accumet meters Important points to remember when choosing pH buffers are to make sure that they encompass the typical pH range that you expect your samples to fall within and to never calibrate at points more than 3 pH units apart calibrating at 4 and 10 for example will not give good results Always calibrate at pH7 regardless Q How regularly should calibrate A The meter should be calibrated regularly using fresh buffers If used on a daily weekly basis then this should be before each use If the meter is in constant use throughout each day then it may better calibrating midway through each day as part of a calibration routine 0 Will the temperature of my sample be an issue A The pH value of any sample varies with temperature so for accurate readings it is always best to measure the temperature too If you are measuring at a different temperature to which you calibrate at it may be worth considering an ATC automatic temperature compensation probe or an electrode with one built in to measure this Modern pH meters will adjust the slope value of the electrode as the temperature changes ensuring the readings stay accurate pH Values
19. Sodium acetate concentrated solution 0 1M ampoule Sodium arsenite solution 0 05M 0 1N ready to use Sodium carbonate solution 0 05M 0 1N ready to use Sodium carbonate solution 0 5M 1N ready to use Sodium carbonate concentrated solution 0 05M 0 1N ampoule Sodium chloride concentrated solution 0 1M 0 1N ampoule Sodium hydroxide solution 2M 2N BP and NIST standard solution free from carbonate ready to use Sodium hydroxide solution 2M 2N BP and NIST standard solution free from carbonate ready to use Sodium hydroxide solution 2M 2N BP and NIST standard solution free from carbonate ready to use Sodium hydroxide solution 0 1M 0 1N free from carbonate ready to use Sodium hydroxide solution 0 1M 0 1N free from carbonate ready to use Sodium hydroxide solution 0 1M 0 1N free from carbonate ready to use Sodium hydroxide solution 0 1M 0 1N free from carbonate ready to use Sodium hydroxide solution 0 2M 0 2N free from carbonate ready to use Sodium hydroxide solution 0 2M 0 2N free from carbonate ready to use Sodium hydroxide solution 0 5M ready to use Sodium hydroxide solution 0 5M ready to use Sodium hydroxide solution 0 5M ready to use Sodium hydroxide solution 1M methanol ready to use Sodium hydroxide solution 1M 1N free from carbonate ready to use Sodium hydroxide solution 1M 1N free from carbonate ready to use Sodium hydroxide soluti
20. ampoule makes 500mL 10000642 Buffer solution pH7 00 phosphate stabilised with 10ppm mercuric chloride Standard Buffer Solution Fisher OHS Buffers Chemical Cat No Description 10593854 Buffer standard solution pH8 00 borate traceable to NIST 10070210 Buffer standard solution pH8 00 borate traceable to NIST 10141620 Buffer standard solution pH8 00 borate traceable to NIST 10164373 Buffer standard solution pH8 00 borate traceable to NIST 10764074 Buffer concentrated solution pH8 00 borate one ampoule makes 500mL 10294480 Buffer concentrated solution pH8 00 borate each ampoule makes 500mL Fisher pH9 Buffers Chemical Cat No Description 10622261 Buffer tablets pH9 2 borate makes 100mL per tablet 10395792 Buffer standard solution pH9 00 borate traceable to NIST 10082531 Buffer standard solution pH9 2 borate traceable to NIST 10142000 Buffer standard solution pH9 2 borate traceable to NIST 10590911 Buffer standard solution pH9 2 borate traceable to NIST 10030180 Buffer standard solution pH9 2 borate traceable to NIST 10419370 Buffer standard solution pH9 2 borat traceable to NIST 10246531 2 Buffer concentrated solution pH 9 2 borate one ampoule makes 500mL 10385750 Buffer concentrated solution pH9 2 borate each ampoule makes 500mL 11472694 Buffer BS standard solution pH9 225 stabilised with 10ppm mercury II chloride traceable to NIST 10395750 Buffer BS standard solution pH9 23 borate
21. can be replaced indefinitely however the periodic requirement for solution top up is inconvenient and also happens to be the main disadvantage Another downside is that when the filling hole is left open for an extended period dried salt may be left behind which often involves cleaning Gel filled electrodes are less expensive require less maintenance and are usually plastic High quality gel formulations have also extended the once limited shelf life in recent years 24 Www eu fishersci com IE UK EN 2014 200 10187 FOCUS ph electrochem master indd 24 08 09 2014 16 15 57 pH Electrodes Single or Double Junction TRIS compatible This decision is extremely important and should not be overlooked If you will be measuring samples that have sulfides proteins heavy metals TRIS or anything that might react with silver or if you will be testing samples that are unknown use a double junction electrode Calomel electrodes would also be suitable but have fallen out of favour due to their mercury content Single junction electrodes are less expensive but offer no other advantages If you use a single junction electrode in a solution with TRIS it s just a matter of time before it fails For dirty samples and those high in particulates sleeve type junctions will offer the longest life span Whilst other electrodes can become clogged and unresponsive with these sample types the sleeve type junction reduces the risk of clogging thereby maximis
22. cells yet and all manufacturers use a different system It is therefore recommended that you stick to conductivity cells from the same manufacturer as your meter 0 Will temperature affect my conductivity measurement A Temperature can have a substantial effect on conductivity Raising the temperature obviously affects the chemical properties of aqueous solutions This in turn contributes to the solution s conductivity Typically conductivity varies by 1 to 3 per degree C Q How should store my conductivity cell A Conductivity cells require minimal storage compared to other electrode types They can be stored in deionised water in between measurements For overnight storage they can be simply rinsed in deionised water and then stored dry Q When should I calibrate my conductivity meter A This should be done on a regular basis before each use if possible possibly as part of a daily calibration routine 43 www eu fishersci com 08 09 2014 16 20 51 Fisher Chemical 44 Indicators Cat No 10101760 10070070 10080070 10213330 10314980 10418980 10030080 10283850 10234340 10486870 10548230 10476870 10284340 10549190 10579000 10060270 10020280 10695112 10214110 10615502 10579190 10060300 10274200 10191620 10765921 10090470 10468420 10366340 10488610 stains Cat No 10325900 10274290 10466870 10598040 10101480 10315
23. digital Traceable V Data logger Iraceable M e Complete DAS 4 System captures and stores up to 8 000 bytes over 1 000 readings e Readings may be taken at intervals from 1 second to 99 hours e Can be read as is or imported to spreadsheets databases and statistical programs e For use with Traceable meters featuring computer output These include Cat No s 11729775 11769785 11789825 11714206 11714216 11724216 11704226 11774276 refer to www eu fishersci com for further information Cat No Description Pack qty 11784276 Data logger Includes Windows CD and a 914mm _ 1 cable USB serial and instrument connections www eu fishersci com IE UK EN 2014 200 10187 FOCUS ph electrochem master indd 65 Fisherbrand Supplement 65 08 09 2014 16 22 32 Fisherbrand Supplement Timers Barometer and clock workstation Iraceable M e deal for recording ambient changes in the lab e Displays temperature and time of day date e May be wall mounted e Barometric pressure range 23 62 to 31 01 inHg 800 to 1050 mbar e Resolution 0 063inHg 1mbar Accuracy 6 mb 890 to 1020 mb e Temperature range 9 to 70 C 22 to 158 F e Resolution 0 5 Accuracy 1 C between 32 to 104 F 0 to 40 C e Dimension mm 197 x 63 5 x 76 2 Cat No Description Pack qty 15273966 Barometer and clock workstation Traceable 1 Stopwatch wateroroof shockproof Iraceable M e Waterproof to
24. materials e Easy to use just insert sample and seal by twirling the bag away from you four times and bending wire ends inward e Choice of round wire or flat wire closures flat wire closures provide extra strength to seal larger and heavier samples e Available in a variety of sizes and with clear or printed write on labels Cat No Capacity mL Pack qty Clear unlabelled with round wire closure 11914385 60 500 11924385 120 500 11904395 3550 900 11944395 710 500 11964405 800 500 11984405 1 000 500 11954405 1 200 500 With writing patch and round wire closure 11944405 60 500 11954385 120 500 11914395 530 500 11974395 710 500 13108187 1 800 250 12309185 3 000 250 Clear unlabelled with flat wire closure 12319185 530 900 13158167 710 500 11974405 1 200 500 12329185 1 600 250 With writing patch and flat wire closure 11984395 D30 500 11904405 710 500 11984385 1 600 250 www eu fishersci com IE UK EN 2014 200 10187 FOCUS ph electrochem master indd 61 Fisherbrand Supplement 61 08 09 2014 16 22 09 Fisherbrand Supplement Containers specimen polypropylene autoclavable e For use with liquid semi solid and solid samples e Inert to most chemicals e Graduated e With or without non autoclavable polyethylene snap on lid e Sterile containers with lids are individually wrapped e Non sterile and sterile containers without lids are bulk packed Cat No Lid Sterile Yes No Colour
25. mercury Il chloride traceable to NIST 10498020 Buffer colour coded concentrated solution pH7 00 phosphate yellow stabilised with 10ppm mercury Il chloride each ampoule makes 500mL 10774074 Buffer colour coded solution pH10 00 borate blue traceable to NIST 10284240 Buffer colour coded solution pH10 00 borate blue traceable to NIST 10723991 Buffer colour coded solution pH10 00 borate blue traceable to NIST 10102100 Buffer colour coded solution pH10 00 borate blue traceable to NIST 10459750 Buffer colour coded solution pH10 00 borate blue traceable to NIST 10293862 Buffer colour coded concentrated solution pH10 00 borate blue one ampoule makes 500mL 10615112 Buffer colour coded concentrated solution pH10 00 borate blue each ampoule makes 500mL Fisher oH2 Buffers Chemical Cat No Description 10703324 Buffer standard solution pH2 00 glycine stabilised with 10ppm mercury II chloride traceable to NIST 10578040 Buffer standard solution pH2 00 glycine stabilised with 10ppm mercury Il chloride traceable to NIST 10588040 Buffer standard solution pH2 00 glycine stabilised with 10ppm mercury I chloride traceable to NIST 10080632 Buffer concentrated solution pH2 00 glycine stabilised with 10ppm mercury II chloride one ampoule makes 500mL 10214390 Buffer concentrated solution pH2 00 glycine stabilised with 10ppm mercury I chloride each ampoule makes 500mL Fisher OHS Buffers Chemical
26. nennen rennen tnnt tnn dd Two or More BUNG S i vsteis todo even cunts Urbe ed YE tenu ne Y Ven Wa pe oy le e Pe P ed NA NEE DIEN cU FAM wirt d Meist TT 34 e pH Electrode Measurements sss 35 Se Mt ce a ae treet vik a eyed dean dp P ie DH Measurement rN TEE aAa a Aaaa A Aa Niana aiaa N eiiis 35 Measurement RecornrernmellOlIS a uscccn usua cucuk carte ctu deck tenete sanata eau E EAR E ERTAN E a 35 e DH Electrode Maintenance sss 36 x29 Oe ils Gia stis ha MN m Tm 36 Filling and Draining a Refillable pH Electrode eerte 36 pH Electrode Storage osse teet 37 Short Term Electrode Storage up to one week nnne ar Long Term Electrode Storage more than one WEEK ccccccsscsssscssessssssssessssseesssecesseceesessesesseseesseeesesessess 37 e pH Electrode Cleaning Procedures sss af e General Rules and Tips sss tetti 38 e Troubleshooting Guide 1 1 ssec 39 40 e Frequently Asked Questions FAQ s sss 41 43 FISHER CHEMICAL tee 44 46 FISHERBRAND SUPPLEMENT ttn 47 e Sample Collection 17T tette 47 51 e Sample Identification sss tetti 52 e Sample Preparation 1 7 sss tette 53 61 e Sample Storage 1 55515S1 sse tette tette ttt ttt 61 62 e Sample Transportation 1 ce cccecsceseseescessesecstesesevscsesevevecstsaseve
27. pack of 20 inner pack of 10 inner pack of 20 Multichannel Cat No 11815772 11825772 11835772 11845772 11855772 11865772 11875772 11885772 Graduations mL Colour No No No Yes Yes Yes No Yes Yes Yes No No No No Watch our SureOne filter tip reload system and Elite pipettors video to discover more mm 104 150 m 150 150 150 155 155 155 155 150 150 300 170 Inner pack qty Yellow 1 Green 1 Orange 1 Yellow 1 Green 1 Blue 1 Orange 1 Red 1 Purple 1 Yellow 25 Green 25 Blue 50 Orange 25 Purple 25 Sterile Length Drop Volume pL 1 to 10 2to 20 5 to 50 10 to 100 20 to 200 100 to 1 000 500 to 5 000 1 000 to 10 000 30 30 30 30 30 30 DB 25 25 25 30 50 20 18 Pack qty 1 000 500 200 1 000 500 200 200 200 100 1 000 500 500 500 100 Drop per mL volume pL 108 33 EE E ER EE 40 40 40 40 E 33 50 ob Pack qty LL Il www eu fishersci com Pack qty 400 500 500 500 500 500 500 500 500 500 500 500 100 200 08 09 2014 16 21 50 Fisherbrand Supplement E a p Co Pipettor tips filter universal fit SureOne e Sterile packaging with outer sleeve protects tips from exterior contamination Cat No Volume pL Pack type Colour Inner Pack qty 10pL micropoint tip graduated at 2pL 11903466 0 1to 10 Filtered sterile Clear
28. ph electrochem master indd 20 www eu fishersci com 08 09 2014 16 15 23 Probes and Accessories Probes and Other Accessories for accumet Meters accumet Benchtop Stirring Probe e Provides mixing without the need for magnetic stir bars or stir plates e Saves valuable bench space e Quiet operation e Adjustable speed control e No additional power required e Multi parameter XL benchtop models can accept up to two probes for simultaneous stirring e Compatible with the following models XL150 XL200 XL250 XL500 XL600 AB150 AB250 AB200 Cat No _ Description 12860653 accumet benchtop stirring probe 12840653 Replacement paddle with stirring rod 11510234 Electrode holding arm and bracket for AB amp XL series benchtop meters Conductivity Dissolved Oxygen and Temperature Probes Conductivity temperature electrodes for AB200 XL200 XL500 and XL600 benchtop meters Pose du M mem ma Cat No _ Sensor Type _ Body Type Nominal Cell Constant Ideal Range 11550164 ug mmm 11540164 2 Cell Plastic 2071 0 5to2 00005 MG 11550164 2 Cell Plastic 1 20uSto20mS 11530164 ELLEN oo 11530164 2 Cell Plastic 10 1 to 200mS at 10236449 4 Cell Glass 1 10S to 100mS 10236449 H B m onn 11560164 4 Cell Glass 10 Po 200mS 11530084 4 Cell Plastic E 10S to 100MS 11560164 HE a a 11540084 4 Cell Plastic 10 1 to 200m mm 153080 B BM messe 12 Cell for accumet AP7
29. redox indicator Methyl orange pure solution 0 04 C I 13025 indicator grade Methyl orange xylene cyanol FF pure indicator grade Methyl red solution 0 01 pure spirit soluble C I 13020 indicator grade Methyl red pure solution 0 025 indicator grade Methyl red pure solution 0 01 indicator grade Methyl violet pure solution 1 CI 42555 indicator grade Phenolphthalein solution 0 296 in industrial methylated spirit IMS Phenolphthalein solution 0 2 in industrial methylated spirit IMS Phenolphthalein solution 1 in industrial methylated spirit IMS Phenol red pure solution 0 02 in water indicator grade Thymol blue pure 0 0496 solution indicator grade Universal indicator pH range 4 to 10 for pH measurement Universal indicator pH range 4 to 10 for pH measurement Full range indicator pH range 1 to 13 for pH measurement Full range indicator pH range 1 to 13 for pH measurement Fisher Chemical Description Benedict s reagent pure qualitative Benedict s reagent pure quantitative Canada balsam pure dried in xylene Fehling s solution No 1 pure to B P 1988 Fehling s solution No 2 pure to B P 1988 Fehling s solution No 1 pure to Lane and Eynon modification Fehling s solution No 2 pure to Lane and Eynon modification Folin amp Ciocalteu s phenol reagent pure Leishmans stain pure in 100 methanol Millon s reagent pure Nessl
30. the electrode is refillable uncover the fill hole during measurements to ensure a uniform flow of filling solution The filling solution level inside the electrode must be at least one inch above the sample solution level e he sample solution level must be above the pH electrode reference junction when the electrode is immersed in the sample e Between measurements rinse the electrode with distilled water and then with the next solution to be measured To reduce the chance of error due to polarisation avoid rubbing or wiping the electrode bulb Use a lint free tissue and gently blot the bulb e Use a magnetic stir plate and stir bar or the accumet benchtop stirring probe to stir all buffers and samples at a moderate uniform rate The stirrer probe can be used with the AB and XL series of benchtop meters e Place a piece of insulating material such cardboard between the magnetic stir plate and beaker to prevent measurement errors from the transfer of heat to the sample e f the electrode is refillable and the electrode is used in dirty or viscous samples or the electrode response becomes sluggish empty the electrode completely and hold the reference junction under warm running water Empty any water from the electrode and fill it with fresh filling solution For a more thorough cleaning refer to the pH Electrode Cleaning Procedures section page 37 e Flat surface electrodes may be used on any moist surface or in liquids e Handle micro p
31. to 50 readings Model No AP75 AP85 Range conductivity 0 0mS cm to 199 9mS cm ico Range TDS 0 00ppt to 200ppt Accuracy conductivity 1 full scale Range C 0 0 to 100 0 Temperature Resolution C 0 1 Accuracy C 0 5 0 2 Range pH 2 00 to 16 00 Resolution pH 0 01 pH Accuracy pH 0 01 pH calibration 5 1 68 4 01 7 00 10 00 12 45 Memory Stores 50 data sets General Cell constant 0 1 1 10 1 Calibration Up to 5 points Cat No Description 11550254 AP75 Meter Includes meter conductivity TDS temp probe Cat No 11346192 batteries and instruction manual 11540254 AP75 Kit Includes meter conductivity TDS temp probe Cat No 11346192 conductivity calibration solutions sample bottle batteries instruction manual and hard plastic carrying case 11510254 AP85 Meter Includes meter pH ATC electrode Cat No 11570174 conductivity TDS temp probe AAA l batteries and manual 11500254 AP85 Kit Includes meter pH ATC electrode Cat No 11570174 conductivity TDS temp probe Cat No 11346192 pH buffer solutions conductivity calibration solutions AAA batteries and manual Replacement Parts and Accessories for AP 5 and AP85 Meters accumet airmen 5 E j 11570174 Cat No Description 11570174 Replacement pH ATC probe plastic body for AP80 and AP70 series 762mm 30 inch cable CT RR 11346192 Replacement conductivity TD
32. traceable to NIST oH1 O Buffers f Chemical Cat No 10789234 10429560 10214200 10274240 10132050 Description Buffer standard solution pH10 00 borate traceable to NIST Buffer standard solution pH10 00 borate traceable to NIST Buffer standard solution pH10 00 borate traceable to NIST Buffer standard solution pH10 00 borate traceable to NIST Buffer concentrated solution pH10 borate each ampoule makes 500mL www eu fishersci com IE UK EN 2014 200 10187 FOCUS ph electrochem master indd 30 Quantity mL 500 1 000 2 500 1 000 6x100 Quantity mL 90 tablets 900 1 000 2 900 5 000 10 000 100 6x100 900 Quantity mL 500 1 000 2 500 10 000 100 6x100 Quantity mL 90 tablets 2500 900 1 000 2500 5 000 10 000 100 6x100 1 000 1 000 Quantity mL 500 1 000 2000 5 000 6x100 For up to date GHS information on Fisher Chemical products listed please refer to the safety data sheet available from www eu fishersci com 08 09 2014 16 16 14 pH Accessories Full Range Indicators Cat No 2 Description Fisher Chemical 10366340 Full range indicator pH range 1 to 13 contains methylated spirit 10488610 Full range indicator pH range 1 to 13 contains methylated spirit Universal Indicators Cat No Description Chemical 10090470 Universal indicator pH range 4 to 10 contains methylat
33. vials 38mm cup diameter for 120 watt sonicator 12706908 Cup horn for indirect processing of up to 8 2mL tubes vials 63mm cup diameter for 500 watt sonicator 12357338 Eight tip horn positioner 3 1mm tip diameter for 120 watt sonicator 12961161 Footswitch for hands free operation of 50 watt sonicator 12981171 Microtip probe 1 6mm diameter 0 2mL to 5mL processing volume for 700 watt sonicator 12921181 Microtip probe 1 9mm diameter 0 2mL to 5mL processing volume for 50 and 120 watt sonicators 12991171 Microtip probe 3 1mm diameter 0 5mL to 15mL processing volume for 500 and 700 watt sonicators 12911181 Microtip probe 3 1mm diameter 0 5mL to 15mL processing volume for 50 and 120 watt sonicators 12931181 Microtip probe 6 3mm diameter 5mL to 50mL processing volume for 120 watt sonicator 12901181 Microtip probe 6 3mm diameter 5mL to 50mL processing volume for 500 and 700 watt sonicators 12921171 Probe 12 7mm diameter 10mL to 250mL with replaceable tip for 500 and 700 watt sonicators 12971161 Probe 19 05mm diameter with replaceable tip 25mL to 500mL processing volume 12901171 Probe 25 4mm diameter with replaceable tip 5 mL to 1000mL for 700 watt sonicator 12951171 Replacement tip 12 7mm diameter for 500 and 700 watt sonicators 12961171 Replacement tip 19 05mm diameter for 500 and 700 watt sonicators 12911171 Solid tip probe 12 7mm diameter 10mL to 250mL processing volume for 500 and 700 watt sonicators 12981161 Solid tip
34. 0 10696642 10151860 10070220 10429180 10243960 10753694 10121910 10677412 10538620 10254150 10355710 10152050 10244150 10723611 10192000 10274100 10518810 10713611 10734761 10734761 10418030 10529000 10666072 10090250 10528620 10428030 10305810 10645112 10000240 Potassium hydroxide solution 0 1M 0 1N methanol ready to use Potassium hydroxide solution 1M 1N alcoholic ready to use Potassium hydroxide solution 1M 1N aqueous ready to use Potassium hydroxide concentrated solution 0 1M 0 1N aqueous ampoule Potassium hydroxide concentrated solution 1M 1N aqueous ampoule Potassium iodate solution 0 05M ready to use stabilised with 10ppm mercury I chloride Potassium permanganate solution 0 02M 0 1N ready to use Potassium permanganate solution 0 02M 0 1N ready to use Potassium thiocyanate solution 0 1M 0 1N ready to use Silver nitrate solution pure 0 025M 0 025N ready to use Silver nitrate solution 0 0282M 0 0282N ready to use Silver nitrate solution 0 02M 0 02N ready to use Silver nitrate solution 0 1M 0 1N ready to use Silver nitrate solution 0 1M 0 1N ready to use Silver nitrate solution 0 1M 0 1N ready to use Silver nitrate solution 0 5M 0 5N ready to use Silver nitrate solution 1N ready to use Silver nitrate concentrated solution 0 1M 0 1N ampoule
35. 0 Microcentrituge tube rack e Each rack can hold 4 x 50mL conical tubes 12 x 15mL conical tubes 32 x 1 5mL microtubes or 32 x 0 5mL microtubes e Rack measures 174mm x 95mm x 52mm e Autoclavable Cat No Description Pack qty Four way tube rack assorted colours EQUUS blue green pink yellow and orange Rota Hack e Each module of the small Rota Rack holds 6 x 15mL tubes 9 x 1 5 2mL tubes 12 x 0 5 0 6mL tubes or 32 independent 0 2mL PCR tubes or 4 x 8 tube strips e Each rack has modules in green pink blue and yellow and is fully autoclavable Cat No Description Pack qty 11394085 Rota Rack M micro tubes each www eu fishersci com IE UK EN 2014 200 10187 FOCUS ph electrochem master indd 54 08 09 2014 16 21 19 Dialysis tubing NEW Regenerated cellulose dialysis tubing e Available range 3 500 to 14 000Da e Pack includes two locking membrane clamps and a pair of surgical scissors e Tubing is supplied on a compact polystyrene spool that eliminates adsorption of glycerin from membranes e Dispenser box is packaged in a plastic bag with a desiccant for humidity control Cat No Description Pack qty 15203857 Dialysis tubing 14 6mm diameter 30m roll 6 to 8kDa MWCO 1 15213857 Dialysis tubing 20 4mm diameter 30m roll 6 to 8kDa MWCO 15223857 Dialysis tubing 25 5mm diameter 30m roll 6 to 8kDa MWCO a 15233857 Dialysis tubing 31 8mm diameter 30m roll 6 to 8kDa MWCO i 15243857 Dia
36. 0 2 11899650 5 11809660 10 11859660 50 e Graduated or non graduated e Sterile options available e Various packaging formats Cat No 13469118 13499108 13439118 13489108 13419118 13429118 13439108 13469108 13479108 13409118 13459118 13459108 13449108 13449118 Description Transfer pipette PE 1mL Transfer pipette PE 1mL graduated Transfer pipette PE 1mL graduated Transfer pipette PE 1mL graduated Transfer pipette PE 1mL graduated Transfer pipette PE 1mL graduated Transfer pipette PE 3mL graduated Transfer pipette PE 3mL graduated Transfer pipette PE 3mL graduated Transfer pipette PE 3mL graduated Transfer pipette PE 4mL thin stem Transfer pipette PE 4mL Transfer pipette PE 7mL extra long Transfer pipette PE 10mL jumbo Pipettors manual Elite e Soft touch tip ejector e Optimal fit to SureOne pipettor tips e Fully autoclavable e Comfortable lightweight handle with finger rest e Extremely low plunger forces Cat No 11815762 11825762 11835762 11845762 11855762 11865762 11875762 11885762 single channel Volume pL 0 2 to2 UE 1to 10 a 2 to 20 1 a 1 Pack qty 5 to 50 10 to 100 20 to 200 100 to 1 000 IE UK EN 2014 200 10187 FOCUS ph electrochem master indd 56 0 01 0 01 0 1 0 01 0 01 0 1 0 1 NE 05 0 01 0 01 0 1 0 1 qub extended tip inner pack of 10 inner
37. 00 phthalate red stabilised with 10ppm mercury II chloride traceable to NIST 10427260 Buffer colour coded solution pH4 00 phthalate red stabilised with 10ppm mercury Il chloride traceable to NIST 10070170 Buffer colour coded solution pH4 00 phthalate red stabilised with 10ppm mercury II chloride traceable to NIST 10774371 Buffer colour coded solution pH4 00 phthalate red stabilised with 10ppm mercury Il chloride traceable to NIST 10080200 Buffer colour coded solution pH4 00 phthalate red stabilised with 10ppm mercury Il chloride traceable to NIST 10164863 Buffer colour coded concentrated solution pH4 00 phthalate red stabilised with 10ppm mercury II chloride one ampoule makes 500mL 10090200 Buffer colour coded concentrated solution pH4 00 phthalate red stabilised with 10ppm mercury II chloride each ampoule makes 500mL 10000642 Buffer colour coded solution pH7 00 phosphate yellow stabilised with 10ppm mercury II chloride traceable to NIST 10477830 Buffer colour coded solution pH7 00 phosphate yellow stabilised with 10ppm mercury Il chloride traceable to NIST 10274480 X Buffer colour coded solution pH7 00 phosphate yellow stabilised with 10ppm mercury Il chloride traceable to NIST 10171570 Buffer colour coded solution pH7 00 phosphate yellow stabilised with 10ppm mercury Il chloride traceable to NIST 10131620 Buffer colour coded solution pH7 00 phosphate yellow stabilised with 10ppm
38. 11779378 Yes Yes Translucent 11769378 No Yes Translucent 11964395 No No Translucent 11799378 Yes Yes Translucent 11789378 Yes Yes Translucent 11984375 No No Translucent Cat No Description 11924395 Lids for specimen containers 11974385 Lids for specimen containers Containers storage Tubby Capacity mL Pack qty 130 500 130 500 130 500 240 500 240 500 240 500 Sterile Yes No Pack qty Yes 500 No 500 e Stackable storage containers to help with laboratory organisation or transportation of products e Removable dividers e For keeping gloves tubes tips pipettes and other small items organised and tidy e Stackable and proportionately sized to utilise space efficiently Cat No Description 11938014 Tubby with 5 tubs 5 lids and 15 dividers SAMPLE TRANSPORTATION Disposable carriers Lab Caddy e Laminated cardboard holds up to 72 petri dishes 20 flasks 75cm e White Cat No Description 11938004 Disposable flask carrier 11928004 Disposable petri dish carrier Trays utility e Lightweight solution for transporting tubes and smaller items Cat No Description 11740634 Utility tray with 13mm OneRack 11750634 Utility tray with 16mm OneRack 62 IE UK EN 2014 200 10187 FOCUS ph electrochem master indd 62 Dimensions Pack qty wxdxh mm 330x200x115 E Pack qty Pack qty a a www eu fishersci com 08 09 2014 16 22 23 THERMOMETERS AND TIMERS Thermometer
39. 132 74x168 81x201 Pack qty P 72 T 48 24 500 250 125 50 Pack qty 72 72 X 48 24 500 250 125 50 Pack qty 72 72 R 48 24 Pack qty 72 72 EP 72 48 24 Pack qty R 72 2 EL 48 24 Bottles wide neck LDPE translucent e Clear bottles with blue polypropylene screw closure e eakproof Cat No 11547443 11553232 11563232 11583232 11593232 11513242 Capacity mL 30 60 125 250 500 1 000 Neck size 33 R3 33 R3 38 R3 58 R3 58 R3 70 R3 IE UK EN 2014 200 10187 FOCUS ph electrochem master indd 49 Height mm nb 80 93 414 M0 200 0 D mm Pack qty 36 12 36 12 50 12 6l 12 76 NI 90 6 www eu fishersci com Fisherbrand Supplement 498 08 09 2014 16 21 05 Fisherbrand Supplement 00 IE UK_EN_2014_200_10187_FOCUS_ph_electrochem_master indd 50 Carboys Cat No Carboy 13458029 13478029 13448029 13468029 Carboy with Spigot 13438039 13408039 13418039 13428039 Capacity mL 900 2 000 900 2 000 900 2 000 900 2 000 material HDPE HDPE pP PP HDPE HDPE PP PP Pack qty I I I Oylinders graduated with spout e arge rounded bases ribbed for reinforcement and stability e Calibrated To Contain To Deliver at 20 C meets ASTM laboratory standards e Chemical heat and impact resistant makes these cylinders excellent for long term use e No menis
40. 5 and AP85 portable meters 11346192 2 Cell Ultem 4 20yS to 20mS 11540084 H E Dissolved oxygen probes and accessories XL600 benchtop meters Cat No _ Description 11530184 _ Self stirring DO BOD temp probe wm 11530184 11590224 Adapter to connect YSI self stirring DO probes To AB XL series meters 10319657 Membrane kit for 11530184 Includes six membrane caps polishing disk and electrolyte filling solution _ ATC temperature probes Cat No _ Description 10236064 _ATC for XL and AB series benchtop meters 11539144 ATC for AP72 and AP71 portable meters 10319657 11590224 11540234 _ ATC for AP100 series portable meters All temperature probes are stainless steel and have a 3 ft cable 10236064 21 www eu fishersci com IE UK EN 2014 200 10187 FOCUS ph electrochem master indd 21 08 09 2014 16 15 43 Probes and Accessories t t N N Cables and Other General Accessories z Adapter cables Cat No Description 11530124 Pin plug to BNC input jack Use with metallic electrodes having 2 pin connectors 11520124 Dual pin plugs to BNC input jack Other replacement parts and accessories SW Cat No _ Description ee 12880653 Compact thermal printer for AB and XL series 100 to 240V Includes two rolls paper and four plug types for global use m 12890653 Replacement thermal paper for 12880653 pack of 2 rolls 11510234 Electrode arm and brac
41. 84 Replacement Parts and Accessories for XL Series Cat No Description 11550174 TRIS compatible accuTupH combination electrode double junction glass body refillable 10236064 ATC probe for AB XL series benchtop meters 11550164 Conductivity cell 2 cell with ATC epoxy body for AB200 and XL series meters cell constant 1 20uS to 20mS 11510154 Fluoride electrode solid state ISE combination refillable BNC connection plastic body interferences OH 110mm x 13mm 80 C max temperature 0 02ppm to _ saturated range 11510134 Ammonia electrode gas sensing ISE combination refillable BNC connection plastic body interferences amines metal cations 108mm x 12mm 60 C max tempera ture 0 009 to 1 700ppm range 11530184 DO BOD temp probe self stirring For best results connect optional stirrer probe Cat No 12860658 refer to page 21 for efficient mixing and fast accurate measurements Refer to pages 21 to 22 for further information on the range of probes and accessories for accumet meters 15 www eu fishersci com IE UK EN 2014 200 10187 FOCUS ph electrochem master indd 15 08 09 2014 16 15 05 The accumet AP series of waterproof portable meters provide accurate measurements anytime anywhere Portable Meters Fisherbrand accumet AP Portable Meter Series The AP71 and AP72 meters feature advanced pH meter functions in a waterproof dustproof housing that floats These meters read pH mV or relative mV and tempe
42. 926955 15 40 6x11 500 11936955 20 40 6 x 11 500 www eu fishersci com IE UK EN 2014 200 10187 FOCUS ph electrochem master indd 53 Fisherbrand Supplement S 08 09 2014 16 21 18 Fisherbrand Supplement Centrifuge tubes 15mL and 50mL e A large white marking area makes it easy to label samples e Tubes are sterilised by gamma radiation and are non cytotoxic Centrifuge tubes 15mL e Polypropylene e Available with a blue plug seal closure with a double start thread design that provides a tight secure seal e Graduated in 0 5mL subdivisions from 2mL to 14 5mL 17mm x 119mm e Packed in racks Cat No Closure style RCF x g max Inner pack qty Pack qty 11849650 Flat top 6 000 50 500 11755075 Flat top 6 000 500 11765075 Plug seal 6 000 500 e Polyethylene terephthalate PET e Packed in racks Cat No Closure style RCExg max nnerpackqty Pack qty 11879640 Plug seal 1 800 50 500 Centrifuge tubes 50mL e Polypropylene e Graduated in 5mL subdivisions from 5mL to 50mL 28mm x 115mm All are sterile except 11829650 Cat No Closure style RCF x g max Pack type Inner pack qty Pack qty 11819650 Flat top 9 400 Rack 25 500 11899640 Plug seal 9 400 Rack 25 900 11809650 Plug seal 9 400 Sleeve m 500 11829650 Plug seal 9 400 Sleeve 25 b00 e Polyethylene terephthalate PET e Plug seal Cat No RCF x g max Pack type Inner pack qty Pack qty 11839650 1 800 Rack 25 50
43. 950 10111480 10191520 10655112 10131910 10356330 10376280 10439190 10439190 FISHER CHEMICAL Fisher Chemical offers more than 4 000 chemicals of the highest quality including dry reagents ready made solutions and high purity solvents All chemicals are ISO 9001 2008 certified and undergo rigorous quality assurance and testing procedures ensuring excellent lot to lot and bottle to bottle consistency Supported by a clear and simple grade and application structure choosing the product that best suits your requirements Is easy Below is a list of chemicals commonly used in electrochemical applications Fisher Chemical Description Bromocresol green pure solution 0 04 indicator grade Bromocresol purple pure solution 0 0496 indicator grade Bromophenol blue pure solution 0 04 indicator grade Bromophenol blue pure 0 2 solution in Methylated spirits Bromothymol blue pure solution 0 04 indicator grade Cresol purple pure solution 0 04 96 pH indicator Cresol red pure solution 0 02 pH indicator Dimidium bromide disulfine extra pure SLR blue indicator stock solution Bicarbonate indicator solution pure concentrated stock reagent Bicarbonate indicator solution pure concentrated stock reagent lodine solution Wij s pure indicator grade lodine solution Wij s pure indicator grade Litmus solution pure indicator grade Methylene blue pure solution 0 015
44. 9805 Pipette filler universal blue 2mL each 15239805 Pipette filler universal green 10mL each 15229805 Pipette filler universal red 25mL each 15219805 Pipette controller each Pipette fillers bulb e Moulded rubber bulb approx 60mL capacity with three glass ball valves which can be controlled by finger pressure e No metal parts to corrode and can be used for all liquids except those which attack rubber e Can be used on bulb or cylindrical pipettes from 2mL to 50mL Cat No Colour Pack qty 11890950 Red 1 Pipettes Pasteur glass Cat No Length mL Type Inner pack Oty Pack qty 11546963 150 Unplugged 250 1 000 11566963 230 Unplugged 250 1 000 www eu fishersci com IE UK EN 2014 200 10187 FOCUS ph electrochem master indd 55 Fisherbrand Supplement li bead Ja 08 09 2014 16 21 24 Fisherbrand Supplement C EE 9b Pipettes serological straight polystyrene e Nonpyrogenic and noncytotoxic warranty e DNase and RNase free e Paper plastic unit packaging unit plastic packaging or in bulk e Sterilisation using gamma irradiation Pipettes transfer e Low density polyethylene e Transparent Cat No Capacity mL Pipettes individually wrapped plastic plastic 11819660 1 11879650 2 11839660 10 Pipettes individually wrapped paper plastic 11849181 1 11859181 2 11869181 19 11879181 10 11839181 ps 11889181 90 Pipettes multipack 11889650 1 1186965
45. Basic mini stirrer DNA 1 11527503 Basic mini stirrer Periodic Table a 11537503 Basic mini stirrer White Crystal 1 Magnetic followers set PTFE cylindrical e Set of 18 cylindrical followers in a compartmented box comprising two each of the following sizes 10mm x 6mm 15mm x 4 5mm 20mm x 6mm 25mm x 6mm 30mm x 6mm 40mm x 8mm 60mm x 10mm 70mm x 10mm Cat No Description Pack qty 10226853 Magnetic followers 1 Magnetic follower restrainer e Retrieve magnetic stirring bars easily from vessels with the handheld Spinbar restrainer Cat No Description Pack qty 11532912 Magnetic follower restrainer 1 www eu fishersci com IE UK_EN_2014_200_10187_FOCUS_ph_electrochem_master indd 58 08 09 2014 16 22 04 Fisherbrand Supplement stirrer overhead compact e Compact size ideal for fume hood or enclosed workstation and small volume applications e Brushless DC motor is efficient with less heat and maintenance free e Belt driven transmission is smooth and quiet with high power efficiency e Easy to use intuitive controls stirrer remembers last speed before powered off e Chemical resistant sealed keypad and splashproof housing e Through shaft makes impeller adjustment easy e Adjustable chuck up to 10mm Technical Specification eoa rana IR NN Aladin ts hee ca Nese di Ata ttnct Ad Sects Pete INVENIO VS NE car EE OU ais aeie al a esoaeeasanee Fh cu ipa ott YER ERR USPTO ROUES R E EPIS ORO
46. Description Pack qty 11873460 Thermometer digital refrigerator freezer Traceable 1 Thermometer timer alarm Traceable NEW e Display shows time current temp and alarm temp setting e Curved stainless steel probe e Adjustable viewing angle e Range 32 to 392 F and 0 to 200 C e Resolution 1 e Accuracy is 2 C e Dimensions mm 70 x 76 x 13 Cat No Description Pack qty 11729735 Thermometer Timer alarm Traceable Thermometers digital retrigerator freezer Plus Traceable e Temperature buffered bottle insulates sensor from rapid temperature changes with high and low settable alarms e Traceable to NIST certificate supplied e Temperature range 50 C to 70 C 58 F to 158 F e Resolution 0 1 C and accuracy 1 C e 3m micro cable permits refrigerator doors to close on it e Dimension mm 82 5 x 63 5 x 16 Cat No Description Pack qty 11705853 Thermometer refrigerator freezer Plus 4 Thermometer refrigerator hi accuracy 0 01 Traceable e Reads two locations e g refrigerator and freezer simultaneously e Triple display shows current and max min readings recording time and date of max min readings e Temperature range 50 C to 70 C e Resolution 0 01 C e Accuracy 0 3 C e Dimensions mm 70 x 108 x 19 Cat No Description Pack qty 11725853 Thermometer refrigerator hi accuracy 0 01 1 Traceable www eu fishersci com IE UK EN 2014 200 10187 FOCUS ph electroch
47. Ensure operator is properly trained in measurement technique refer to meter instruction manual for further information accumet Meter Specific Issues Perform two point calibration refer to page 33 e AUTO calibration values 84uS 1413pS 12 88mS or 111 8mS are not used Change Cal Method to MANUAL Only one point per range can be calibrated Re consider your aU SINGLE or MULTI Cal Method setting e Adjust TDS factor as needed to correct value Over range or Under range condition check that electrode is connected Please send a written request with your name contact information and instrument serial number to Fisher Scientific Product Team Email fisheruk productsupport thermofisher com a temporary password will be issued If you still cannot resolve your issue or have any questions at all then Contact our Product Support Advisors Www eu fishersci com 08 09 2014 16 18 51 Technical Resources Frequently asked questions FAQ s his section lists some of the most frequently asked questions about electrochemistry and pH measurement as received by our Life Science and Chemical Specialists together with the answers they provided If you are unable to find the answer to your question are stuck and need help or are simply confused and unsure of which product best suits your research needs the Product Support Team are here and ready to respond to your enquiries Stuck Hav
48. Fisherbrand Fsherbrand Focus QUALITY RELIABILITY VALUE Whatever your application Fisherbrand has a solution for you FLECTROCHEMISTRY FOCUS ON DH Fisherorand and Fisher Chemical working together to deliver reliable and essential products that meet your most demanding electrochemistry requirements IE UK EN 2014 200 10187 FOCUS ph electrochem master indi 08 09 2014 16 11 37 Meet the Fisher Scientific Family Fisher Scientific s trusted well established and proprietary product range Fisherbrand is committed to providing quality products at affordable prices Fisherbrand offers a broad selection of laboratory supplies and consumables covering a diverse range of applications such as chromatography liquid handling electrophoresis pH and electrochemistry It s the smart way to achieve cost savings over branded products without having to compromise on quality Je ywhere your lab Fisherbrand Qua ity RELIABILITY VALUE In addition to the extensive Fisherbrand range Fisher Scientific is your partner of choice for chemicals and bioreagents Fisher Chemical and Fisher Bioreagents deliver convenience quality and consistency and are the leading provider of chemicals and bioreagents to many research sectors such as academia pharmaceuticals biotechnology and healthcare e Fisher Chemical offers more than 4 000 chemicals of the highest quality including dry reagents ready made solutions and high purity so
49. H electrodes with care Do not touch the pH bulb and stem against the bottom or walls of the sample containers www eu fishersci com Technical Resources 35 08 09 2014 16 18 01 IE UK EN 2014 200 10187 FOCUS ph electrochem master indd 36 Technical Resources pH Electrode Maintenance Q TechSupport You Will need pH meter Electrode Wash bottles Beaker Pipettor MITT um P d o 2B pages 10 to 20 pages 27 pages 31 pages 47 pages 56 to 5 Looking After Your Electrode 1 On a weekly basis inspect the pH electrode for scratches cracks salt crystal build up or membrane junction deposits 2 Rinse off any salt build up with distilled water Remove any membrane junction deposits by soaking the electrode in a 0 1M HCI for 15 minutes or soaking the electrode in a 0 1M KCI solution heated to 55 C for 15 minutes If the electrode is dirty clogged or coated refer to the pH Electrode Cleaning Procedures section pages 37 for a more thorough electrode cleaning procedure 3 If a refillable electrode is used drain the reference chamber flush it with distilled water until all of the salt crystal build up inside of the electrode is removed flush it with fresh filling solution and then finally fill the reference chamber with further fresh filling solution 4 Soak the electrode in standard pH electrode storage solution for 1 to 2 hours Filling and Draining a Refillable oH Electrode To fill an electrod
50. LECTION Glassware Beakers squat form e Borosilicate glass e Squat form with spout and graduations e SO 3819 DIN 12331 Cat No 11597392 11507402 11942213 11527402 12907600 11547402 11982213 11567402 11577402 11587402 11507412 Bottles Capacity mL 125 50 100 150 250 400 600 800 1 000 2 000 5 000 50 60 70 80 95 110 125 135 143 189 270 Height mm e Borosilicate glass reagent and media screw neck e Graduated and supplied with blue polyproplyene cap and pouring ring e Chemically resistant Cat No 11962243 11738151 11972243 11982243 11369493 Capacity mL 10 250 500 1 000 2 000 w 143 181 230 267 Height mm Exterior diameter mm 34 42 50 60 70 80 90 100 105 130 170 e Autoclavable Exterior diameter mm 56 70 86 101 136 Cylinders borosilicate glass Class A e Graduated in blue ceramic markings e ndividual reorder code on each item e Pouring spout Cat No 11517832 11527832 11537832 11547832 11557832 11567832 11577832 Capacity mL 10 25 50 100 250 500 1 000 1 0 1 0 Em 50 10 0 e Hexagonal base e DIN 12680 BS 604 ISO 4788 Graduations mL 02 05 Cylinders borosilicate glass Class B e Graduated in blue ceramic markings e Individual reorder code on each item e Pouring spout Cat No 11507702 11517702 11527702 11537702 11547702 11557702 11567702 11577702 11587702 Capa
51. S temperature probe 11346192 Refer to pages 21 to 22 for further information on the range of probes and accessories for accumet meters 19 www eu fishersci com IE UK EN 2014 200 10187 FOCUS ph electrochem master indd 19 08 09 2014 16 15 18 AP100 Series pH Meters Pocket sized portable meters for indoor or outdoor use e Large LCD with backlighting and plain language text making it easy to view and use e Durable waterproof IP67 housing e pH resolution and date time to meet GLP AP115 AP125 e 200 data point internal memory e Three year meter warranty and one year electrode warranty ij Portable Meters L fiL eee peig ends eoe eoe rens Model No AP110 AP115 AP125 Range pH 2 00 to 20 00 2 00 to 20 00 2 00 to 20 00 Resolution pH 0 1 0 01 0 1 0 01 0 001 0 1 0 01 0 001 Accuracy pH 0 01 0 002 0 002 Bn Up to 5 or 6 points from 3 sets T USA 2 00 4 00 7 00 10 00 12 00 ai i NIST 1 68 4 01 6 86 9 18 12 45 EURO 1 00 3 00 6 00 8 00 10 00 13 00 Range mV 2 000 2 000 2 000 mV Resolution mV 0 1 1 0 1 1 0 1 1 Accuracy mV 0 2 2 0 2 2 0 2 2 Calibration mV Calibration points 1Rel mv Range concentration 0 001 to 99 999 lon Resolution concentration 1 2 or 3 significant digits concentra tion Accuracy concentration Monovalent 0 5 full scale Trivalent 1 full scale AP125 only Calibration Availab
52. UD DELETE oth irse d COT E ttem VT ERIT IUE gt EO Cat No Description 15203807 Compact overhead stirrer general purpose mixing Stirrer overhead high torque digital e Durable unbeatable quality TENV cast aluminum housing coated with chemical resistant epoxy paint e Brushless DC motor is efficient with less heat and maintenance free e Belt driven transmission is smooth and quiet with high power efficiency e Adjustable chuck up to 10mm e Chuck guard protects the user from moving chuck and protects the chuck itself from splashes e Electronic speed control two speed transmission maintains set speed at all viscosities e Transmission changes automatically to high torque or high speed range e ero torque feature similar to a tare feature on a scale e Sealed keypad is easy to use intuitive and water tight e Through shaft makes impeller adjustment easy Technical Specification www eu fishersci com IE UK EN 2014 200 10187 FOCUS ph electrochem master indd 59 wc TINI MR Et m 20 to 3 000 Lu SEMI LETS e Made eu MR Rem ges is E E EE 1 15 Maximum volume L NNI Re REIeeeo oecm E E E EE EET A encoun EA AEA E 50 000 Cat No Description Pack qty 15293797 High torque digital overhead stirrer 1 Accessories tor overhead stirrers Cat No Description Pack qty 12977096 Chuck and key set 4 12987096 Safety stand with base 711mm x 25mm diameter rod 1 12907106 Stirrer clamp 1 12917106 Crossed blade impeller 400mm le
53. White Natural Natural Natural Yellow Green Natural Natural Natural Natural Natural pack size 100 sticks 100 sticks 100 sticks 100 sticks 100 sticks 100 sticks pack size Aa HPeP PSP SHH HS SF pack size CO Io o iG IGI CO IG CO Ga O fea Quantity mL 100 500 Quantity mL 100 500 2500 For up to date GHS information on Fisher Chemical products listed please refer to the safety data sheet available from www eu fishersci com IE UK EN 2014 200 10187 FOCUS ph electrochem master indd 31 Wwww eu fishersci com 08 09 2014 16 16 19 Technical Resources TECHNICAL RESOURCES Q TechSupport Here to give you a helping hand Fisher Scientific s Product Support Team is your dedicated information resource Our Product Support Advisors are all highly qualified professionals who are here to support and guide you to the fastest most effective and efficient answer to your enquiry Areas of technical expertise include e Bioreagents and Life Science e Chemicals and Chromatography e Consumables e Equipment e Safety This section features a number of helpful resources such as how to prepare and calibrate your electrode and how to store and clean your electrode as well as a troubleshooting guide and FAQ s If however this information does not resolve your issue or if you have questions not covered below Contact our Product Support Advisors pH Electrode Prepara
54. ae aiaia 0 001 0 01 0 1 1 automatic Cat No Description 12850633 AB250 Meter Includes meter electrode arm RS 232 amp USB cables 100 240V power supply and manual 12860633 AB250 Kit Includes meter TRIS compatible pH ATC electrode Cat No 11500194 electrode arm RS 232 amp USB cables 100 240V power supply and manual 11550164 11500194 10236064 11550174 Replacement Parts and Accessories for AB Series Cat No Description 11550174 TRIS compatible accuTupH combination electrode double junction glass body refillable 11500194 TRIS compatible pH ATC combination electrode double junction plastic body refillable 10236064 ATC probe for AB XL series benchtop meters 11550164 Conductivity cell 2 cell with ATC epoxy body for AB200 and XL series meters cell constant 1 0 sensing range of 204S to 20mS For best results connect optional stirrer probe Cat No 12860653 refer to page 21 for efficient mixing and fast accurate measurements Refer to pages 21 to 22 for further information on the range of probes and accessories for accumet meters 12 www eu fishersci com IE UK EN 2014 200 10187 FOCUS ph electrochem master indd 12 08 09 2014 16 14 53 accumet XL Series e Colour touch screen e wo stirring probe ports e Three position electrode holder e Upgradeable software e USB amp RS232 connectivity pH Meter XL 150 Benchtop Meters Simple yet powerful large full colour touc
55. and the rest of our newly expanded and upgraded range of benchtop meters in this section To view the instruction manuals for the following range of accumet benchtop meters visit www eu fishersci com fisherbrand Selection Guide for Benchtop Meters Find the right accumet benchtop meter for your particular application using the selection guide below Model AB150 AB200 Page 11 11 12 13 13 14 14 15 m Ta SR a aa D NEN pH mV BNC Temp o o o o ee ee ec lon Selective mV BNC Temp e ee ee ee Conductivity TDS Resistivity Salinity Temp e e e e Dissolved Oxygen Temp e Stirring Probe o o ee ee ee ee USB Device RS 232 e e o e e e USB Host RS 232 RJ 45 Ethernet e e e e e ee Dual Channel Capacity www eu fishersci com IE UK_EN_2014_200_10187_FOCUS_ph_electrochem_master indd 10 08 09 2014 16 14 45 Benchtop Meters accumet AB Series e Multiple views e 3 position electrode holder e Backlight display e Stirring capability e Date time for GLP requirements e Upgradeable software e Customer buffer calibration options e Wall mounting available pH Meter AB150 Intuitive simple operation and high accuracy in a compact affordable meter Technical Specification Temperature Mode pH Resolution ree 0 1 C 0 1 F h t 2 000 to 20 000pH ws eT 0 3 C 0 5 F Re Onom 5 Saxpebohstdcbostddbdeiimqaddeadta idus dd 0 1 0 01 0 001 pH Calibration s
56. articular application _ Single parameter Portable Meter Dual parameter Single parameter Benchtop Meter CUR pH Conductivity DO pH Dual parameter pH ISE Conductivity Multi parameter pH ISE D0 IE UK EN 2014 200 10187 FOCUS ph electrochem master indd 9 Conductivity single Buffer Set Multiple Buffer Set Intermediate Advanced pH Conductivity pH ISE Dual BNC Dual BNC ecc 29 9 www eu fishersci com Graphical LCD Display Colour Touchscreen single BNC single BNC Dual BNC Colour Touchscreen Colour Touchscreen Standard Meter Intermediate Meter Advanced Meter Graphical LCD Display Colour Touchscreen Graphical LCD Display Colour Touchscreen 08 09 2014 16 14 09 Benchtop Meters Fisherbrand accumet AB and XL Benchtop Meter Series The Fisherbrand accumet AB and XL series of benchtop meters are available either as meters only which also include the electrode arm RS232 and USB cables power supply and user manual or else as a kit which includes the meter appropriate electrode and probes electrode arm RS232 and USB cables power supply and manual Within this range the AB150 is Fisher Scientific s top selling pH meter It is the perfect choice for all laboratories as an accurate easy to use pH mV meter with exceptionally high accuracy and resolution Find out more about the AB150
57. as others Usually in situations in which a pH electrode didn t last long the electrode is not matched well for the application resulting in poor performance and ultimately failure Understanding the different electrode options that are available and knowing how to use them to your advantage is a critical step to getting the most out of your pH measurement system The following section will provide you with an overview of the different types of electrode available for use with the accumet range If however you have any further questions concerning electrode selection then please contact our Product Support Advisors Combination or Half Cell There are two components within a pH electrode system A pH indicating or sensing electrode which develops a potential dependent on the pH of the sample and the reference electrode which provides a constant voltage for the meter to compare against These two cells also help complete the electrical circuit between the meter and the sample Combination electrodes have both the indicating and reference electrodes combined into one electrode Alternatively separate half cell electrodes can be used Since reference electrodes are often outlasted by sensing electrodes you only need to swap out one electrode at a time and this can mean lower costs compared to replacing with an equivalent combination electrode each time In practical terms nearly all electrodes used today are combination electrodes Th
58. ases so the pH value decreases Conversely when the pH is above 7 the solution is described as basic or alkaline because the activity of hydroxide ion is greater than that of hydrogen ion pH can be measured in a number of different ways These include basic tests such as using test papers litmus paper for example and chemicals e g universal indicators through to using more sophisticated items of equipment such as colorimeters and photometers and electrochemical sensors This brochure will focus on electrochemistry the most accurate of all the methods Www eu fishersci com 08 09 2014 16 14 08 Electrochemistry Meters ACCUMET ELECTROCHEMISTRY METERS This next section provides an overview of the comprehensive range of Fisherbrand accumet electrochemistry meters and accessories These instruments represent the state of the art for measuring pH mV ORP ion concentration conductivity total dissolved solids TDS dissolved oxygen and temperature Most of these instruments are capable of measuring more than one of these parameters The Fisherbrand accumet AB series of benchtop meters offer accuracy simplified performance and innovative features such as a backlit display multiple views date time for GLP requirements expanded memory custom buffer calibration options a calibration alarm three position electrode holder stirring capability upgradeable software and the option to mount on a wall The meters can be set to
59. asured to prevent reverse electrolyte flow e When taking measurements the electrode need only be immersed far enough to cover both the glass pH sensing bulb and reference junction to obtain accurate readings e Electrodes perform best when they are hydrated However if they dry out they can be reconditioned to normal performance again Soaking in electrode storage solution helps to optimise and re establish the thin hydration layer on the sensing bulb that is critical to pH measurement e Rinsing the electrode with deionised or distilled water between samples is fine but storage in deionised or distilled can be detrimental as it will rob critical ions from the sensing bulb Also avoid wiping or touching the sensing bulb so as to maintain the hydration layer and avoid producing any electrical charge e Moving or touching the electrode cable may result in unstable readings due to the high impedance resistance of the pH glass membrane and Introduce noise e o eliminate temperature errors associated with the electrode manual or automatic temperature compensation ATC should be used for best accuracy Since temperature changes pH the sample temperature should always be noted with pH readings i e record results as pH8 43 23 2 C instead of just pH8 43 e Always use fresh pH buffers for calibration Excessive air exposure and sunlight can alter the buffer s value especially pH10 buffers which are particularly susceptible to dri
60. at No 12784506 pH electrode Cat No 12865443 electrolyte two membrane cap assemblies Cat No 10030073 hard carrying case polishing disk AAA batteries and manual Replacement Parts and Accessories for AP 4 and AP84 Meters Cat No Description 12784506 10030073 12865443 Replacement dissolved oxygen temperature probe with 10ft submersible cable DO replacement membrane assembly pre assembled membrane membrane lock O ring cap Replacement pH electrode for AP84 ABS body 10ft cable 25mm diameter accumet 12784506 Refer to pages 21 to 22 for further information on the range of probes and accessories for accumet meters 18 IE UK EN 2014 200 10187 FOCUS ph electrochem master indd 18 Www eu fishersci com 08 09 2014 16 15 14 Portable Meters AP75 and AP85 Conductivity Meters Rugged waterproof portable meters e Completely waterproof and dustproof e C F selectable e 1 full scale accuracy for conductivity and TDS measurements e Switch from conductivity and TDS with the press of a button e Dual display shows conductivity or TDS plus temperature e Auto ranging for conductivity across five ranges e Five point conductivity and TDS calibration AP85 meter also features e pH measurement with five point pH calibration and auto buffer recognition AP75 meter also features Real time clock stamps stored data and calibration data with date and time e Memory function stores and recalls up
61. chnique Operator technique refer to meter instruction manual for further information e Whilst Tris buffers can pose a particular problem other samples may also require a specific electrode type Particulate samples sample type and solid semi solid samples may also present a problem Refer to pH Electrode Selection Guide page 26 for further advice or contact Fisher Scientific Product Support Team if unsure 2 39 www eu fishersci com IE UK EN 2014 200 10187 FOCUS ph electrochem master indd 39 08 09 2014 16 18 45 40 Problem Conductivity ISE Display reads Conductivity Can t adjust or can t calibrate E TT aaa _ calibration point replaces TDS Standard does 2 not match OR or UR error message Forgotten password IE UK EN 2014 200 10187 FOCUS ph electrochem master indd 40 Meter Conductivity cell Technique Two point calibration has not been performed Conductivity calibration standards Settings settings Suggestions Conductivity e Perform self test or meter diagnosis programme refer to meter instruction manual for further information s correct cell constant being used for the sample Low and high conductivity samples will likely require a different cell constant to be selected Refer to the table in the FAQ section page 43 for Make sure that reading is being given sufficient time to stabilise
62. cid solution 1M 2N ready to use Sulfuric acid solution 1M 2N ready to use Sulfuric acid concentrated solution 0 05M 0 1N ampoule Sulfuric acid concentrated solution 0 5M 1N ampoule Tetra n butyl ammonium hydroxide solution 0 1 M 0 1N in toluene methanol ready to use Titanium Ill chloride solution 0 2 M 0 2N ready to use Zinc sulfate concentrated solution 0 05M ampoule IE 1L LaL 6 ampoules 6 ampoules IL Je ISI 1L 1h 250mL 1E IE 2 95 10L iS 1L 6 ampoules IL I 1L Zale 6 ampoules 6 ampoules 2 5L oll iL E 2 5L 5L 10L iL 25L E 5L 10L 1L iL 2 5L 5L 10L 6 ampoules 6 ampoules 6 ampoules 1L 6 ampoules E 25E 5L 10L 6 ampoules 25L 1L DE Zak 10L 1L 2DE 10L 2 5L 1L JL 1L 2AE 10L 2al T 6 ampoules 6 ampoules IL 2 5L 6 ampoules For up to date GHS information on Fisher Chemical products listed please refer to the safety data sheet available from www eu fishersci com www eu fishersci com IE UK EN 2014 200 10187 FOCUS ph electrochem master indd 46 08 09 2014 16 20 52 Fisherbrand Supplement This section features key Fisherbrand consumables and apparatus to supplement your pH and electrochemistry needs Once again Fisherbrand demonstrates that it s going that extra mile to continually deliver you quality affordable products SAMPLE COL
63. city mL 10 v5 50 100 250 500 1 000 2 000 1 0 1 0 20 5 0 10 0 20 0 IE UK EN 2014 200 10187 FOCUS ph electrochem master indd 47 e Hexagonal base e DIN 12680 BS 604 ISO 4788 _ Graduations mL 01 0 2 05 NYNNNN NM NM NM lh Pack qty 10 10 eo 10 10 10 10 10 Pack qty 10 10 10 10 Pack qty NRO M9 BR MH NM PL PL Pack qty www eu fishersci com 4 08 09 2014 16 20 54 40 Funnels borosilicate glass conical Cat No Top diameter mm Pack qty 11572423 SE aum 11582423 75 10 11592423 100 a 11502433 150 1 11512433 200 a Funnels borosilicate glass conical powder Cat No Top diameter Stem length Stem O D Pack qty mm mm mm 12983591 50 20 B 1 12993591 70 25 16 12903601 100 2 v 1 Funnels soda lime glass conical Cat No Top diameter Stem length mm Pack qty mm 11502423 35 40 11512423 45 45 11522423 50 50 11532423 55 cE 11542423 60 60 11552423 70 70 11562423 100 100 EM cl ES lo EAM m EY Plasticware Beakers squat form polypropylene ultra clear e Large pouring spout and moulded graduations e Not suitable for stirrer hotplates Cat No Capacity mL Pack qty 11572283 QE 10 11512293 500 10 Beakers tri cornered polypropylene e Suitable for use with commonly used acids alkalis and solvents e Each beaker has three drip free pouring spouts e Moulded graduations stackable Cat No Capacity Subdivisio
64. cus to confuse readings eliminates guesswork Cat No Cylinder PPCO 11947884 11957884 11967884 11997874 11907884 11967874 11937874 11927874 Cylinder PMP 11907894 11917894 11977884 11917884 11977874 11947874 Capacity mL 40 25 50 100 250 500 1 000 2 000 25 50 100 500 1 000 2 000 Funnel polypropylene Cat No Funnel analytical 12652425 12662425 12672425 12682425 12692425 12602435 12612435 Funnel powder 12622435 12632435 12642435 12652435 Funnel utility 12662435 12682435 12672435 12692435 12602445 12622445 Capacity mL ad 23 37 50 100 150 225 50 100 225 750 40 100 140 210 410 500 Pack qty 12 12 12 8 NA oO cO 18 12 Pack qty 36 36 36 36 36 24 94 36 36 24 24 72 72 2 R 24 2 www eu fishersci com 08 09 2014 16 21 08 Petri dishes plastic round e Inner packs of 20 Cat No B x H mm Vent Pack qty Aseptic 12694785 90x 142 B 600 12664785 90 x 16 2 E 600 12644785 90x 162 0 600 12654785 90 x 16 2 1 600 Sterile 12604795 90x 142 29 600 12674785 90x 16 2 3 600 12684785 90 x 16 2 1 600 Scoops polypropylene Cat No Capacity mL Length Pack qty 2 overall mm 11567852 25 9b 25 11577852 50 160 5 11587852 100 200 5 11597852 250 260 1 11507862 H 315 11517862 1 000 385 1 owabs e All swabs have a round compact and shred resistant head for sample taking e Prod
65. d can usually take much more abuse in the lab and in the field Most electrodes with built in temperature compensation elements are plastic due to the complexity in manufacturing them As a result they are most popular with field and portable meters but can also be used in laboratory environments To protect the glass sensing bulb many plastic electrodes use an integral housing that limit the bulb exposure but often can be difficult to clean In addition to the body material of the electrode different body styles are also available for use when measuring certain sample types As well as the standard familiar electrode shape the Fisherbrand range incorporates spear type electrodes for measuring semi solids meats cheeses etc thin stem semi micro electrodes for small samples and where space is limited and the Tuff Tip electrodes for when a more robust electrode is necessary Refer to pH Electrode Features page 25 for further information Refillable or Non Refillable All pH electrodes use and inevitably leak solution Refillable electrodes do so more quickly but can be easily replenished when they require more filling solution However gel filled electrodes leak very slowly but when they run out or the gel is no longer flowing cannot be replenished and must be replaced Refillable electrodes are generally more expensive than gel filled equivalent electrodes but respond much faster They also last longer because the filling solution
66. display local language and feature user prompts and context specific help screens The AB150 from this range is Fisher Scientific s top selling pH meter and is the instrument of choice for routine laboratory pH needs Two further models have recently been added to this range the AB250 with ISE capability and the AB200 the first AB multi parameter instrument for both pH and conductivity The Fisherbrand accumet XL series of benchtop meters bring more advanced designs including a large vivid colour touchscreen with multiple channels and new features including two stirring probe ports a three position electrode holder upgradeable software and USB and RS232 connectivity making these popular meters better than ever Similar to the AB series the XL series can be set to display local language and feature user prompts and context specific help screens The Fisherbrand accumet AP series of handheld portable meters are ideal for the researcher on the go For both indoor and outdoor use the AP series feature soft touch keys with comfort grip making one handed operation a breeze Other features include rugged waterproof housing IP67 rated a large LCD screen with backlighting and easy to use plain language text The accumet AB XL and AP series of pH meters all offer high quality user friendly and cost effective instruments for every application and budget The diagram below will guide you to selecting the right meter for your p
67. e Cat No 11510154 TRIS compatible accuTupH pH electrode Cat No 11550174 ATC probe Cat No 10236064 electrode arm RS 232 amp USB cables 110 220V power supply and manual 12890643 XL250 Ammonia Kit Includes meter ammonia electrode Cat No 11510134 TRIS compatible accuTupH pH electrode Cat No 11550174 ATC probe Cat No pH Meter XL 500 Multi channel operation simplifies your lab work 10236064 electrode arm RS 232 amp USB cables 110 220V power supply and manual Technical Specification lon Mode pH ic e 1x 10 103 99 x 10 MONG dignus ad Daun HIR MES 2 000 to 20 000pH SONNY atutatdzssitisiivitunumeif qe 0 1 0 01 0 001 POU O O O e E A R 0 1 0 01 0 001 pH Reay aiiai 0 5 full scale monovalent ion CUN MEM 40 1 0 01 0 00241LSD G 2QQQQeeeeeeHeH Hmemmmmmeme 196 full scale divalent ion Auto Buffer Recognition 5 preset buffer groups custom Conductivity Mode mV Mode Range n tsk aap ia dimi qM id 0 to 500 0mS HON 8 ddusquendetoiquiuiaeneue missi as 2000mV Rel mV Resolution axsssueiideiud ito 0 01 0 1uS 0 001 0 01 0 1mS sr por Mc 0 1 REPE epsckHbbsndtaedi i a nU Ma E 1 full scale 2 0r c Nr c 0 2 Output ne RJ45 RS232 USB A mini B USB two stirrer ports Temperature Mode Language Selection _ English French Spanish German Italian Chinese 2 5 T0 c 2
68. e install the flip spout cap onto the filling solution bottle and lift the flip spout to a vertical position Insert the spout into the electrode fill hole and add filling solution up to the fill hole If a flushable electrode is used push down on the electrode cap to allow a few drops of filling solution to drain out of the electrode and release the cap to reset the renewable junction Push down and release the cap until the junction returns to its original position and add filling solution up to the fill hole To drain most electrodes insert a lint free tissue or pipette into the fill hole and remove all of the filling solution If a pipette is used do not insert it too far into the electrode and do not touch the inner glass tube with the pipette To drain a flushable pH electrode make sure that the fill hole is uncovered place a waste beaker under the electrode and push down on the electrode cap to remove all of the filling solution The filling solution will flow out of the renewable junction near the pH bulb 36 Wwww eu fishersci com 08 09 2014 16 18 16 IE UK EN 2014 200 10187 FOCUS ph electrochem master indd 37 Technical Resources pH Electrode Storage TechSupport To ensure a quick electrode response and an unclogged electrode junction the electrode should never be stored dry and the pH sensing bulb and reference junction must not dry out Always store the pH electrode in pH electrode storage solution ohort Term Electrode S
69. e a Confused need help question Contact our Product Support Advisors pH Q am looking to test samples containing Tris buffers which electrode shall use A A number of electrodes could be suitable but what s important is that it is a double junction electrode Refer to the pH Electrode Selection Guide on page 26 or further advice Q My electrodes are failing within a short time frame what could be the issue A Not all electrodes are suitable for all sample types Refer to the pH Electrode Selection Guide page 26 or for further advice contact the Fisher Scientific Product Support Team Q I ve heard of some samples should be wary of using standard electrodes in what are they A Standard electrodes use silver ions in their reference system Proteins Tris buffers and general biological samples all react with silver ions and this reaction can lead to the electrode having a shorter life span Q am having trouble calibrating my meter what could be doing wrong A Fresh buffer solutions preferably certified to a known standard should always be used The age of an electrode should also be considered Electrodes have a useful lifetime of approximately 6 months to a year and should be treated as a consumable www eu fishersci com IE UK EN 2014 200 10187 FOCUS ph electrochem master indd 41 08 09 2014 16 20 51 Technical Resources 0 Which pH buffers should I use to calibrate my electrode
70. e accuTupH pH electrode Cat No 11550174 ATC probe Cat No 10236064 electrode arm 110 220V power supply and manual pH Meter AB200 An accurate affordable multi parameter instrument Technical Specification Temperature Mode pH BESOIN Pea idea etiain etia etai Os AON os me MMC eae ae gan TUE PERPE 2 000 to 20 000pH Pole Anninidonppemu pudo dir dM un SU iue M CUN BLTUISDET WEB susuiiei GRADUM IM UAI ENIE 0 1 0 01 0 001 pH COUPEE mapema Offset in 0 1 increments offset range 5 C 9 F BORNE escis cn UR NN REN etn cence niveau ne oi 0 002pH 1LSD BufferSets esses USA 2 000 4 010 6 997 10 013 12 000 Output _ ooossssiudeopidun RS 232 phono plug mini B USB stirrer NIST 1 678 4 010 6 865 9 184 12 460 Language Selection eese ENglish German French Italian amp Spanish EE E DIN 1 090 3 060 4 650 6 790 9 230 12 750 Memory Mirain in ennai eana data sets viewable E FSCI 1 000 3 000 6 000 8 000 10 000 13 000 TDS Mode EE EE eee CUSTOM Any 2 5 values 21 0pH unit apart Range paapaa ssa U DOppm to S00ppt TDS factor 1 00 Slope Display ensseneeee Yes up to 5 different slopes with offset Resolution apia 0 00ppm to 500ppt TDS factor 1 00 Temp Compensation Automatic or manual 0 to 100 C 32 to 212 F 07 1 0 Mr 0 01 0 1ppm 0 001 0 01 0 1ppt Conductivity Mode orac o ZdauatHiobutndHbitduseoE uad cosctetur AE 1 ful
71. ed spirit 10468420 Universal indicator pH range 4 to 10 contains methylated spirit 10705351 Universal indicator pH range 4 to 10 contains methylated spirit OH Indicator Paper Sticks e 85mm x 6mm sticks with different indicator papers sealed onto each strip e The pH is determined by comparison with a colour chart supplied The sticks are sufficiently long to avoid contact between the fingers and the test sample Cat No Description 10642751 pH indicator paper stick non bleeding supplied with colour comparison chart 10018080 pH indicator paper stick non bleeding supplied with colour comparison chart 10017950 pH indicator paper stick non bleeding supplied with colour comparison chart 10353641 pH indicator paper stick non bleeding supplied with colour comparison chart 10333501 pH indicator paper stick non bleeding supplied with colour comparison chart 11386454 pH indicator paper stick non bleeding supplied with colour comparison chart 10271751 pH indicator paper stick non bleeding supplied with colour comparison chart pH Indicator Paper Reels e Dispenser reels each 5m x 7mm I x w except 10433151 which is 5m x 10mm I x w Cat No Description Type 11567382 pH indicator paper reel Litmus red 11577382 pH indicator paper reel Litmus blue 10482341 pH indicator paper reel pH 11507392 pH indicator paper reel pH 11517392 pH indicator paper reel pH 11527392 pH indicator paper reel pH 11537392 pH ind
72. em master indd 64 TIT f richer scionaitie 08 09 2014 16 22 30 Hygrometer thermometer barometer dew point pen ireoceapie e Probe is sealed in glycol solution e Minimum maximum memory e Signals out of range conditions e Dimensions mm 38 diameter x 203 Cat No Description Pack qty 11745853 Hygrometer thermometer barometer dew point pen 1 Traceable V Thermometer digital lollipop waterproof Traceable e Resolution 0 1 C e Accuracy 1 C e Waterproof shockproof e Range 50 C to 300 C 58 F to 572 F Cat No Description 11785853 Lollipop M thermometer Pack qty Thermometer Digital Bottle refrigerator freezer Traceable e Display shows high low and current temperatures e Temperature range 30 0 C to 85 0 C e Resolution 0 1 C e Accuracy 1 0 C between 20 0 C to 85 0 C e Bottle dimensions mm 25 4 x 25 4 x 64 5 e Holder dimensions mm 57 x 57 x 28 6 Cat No Description Pack qty 11709745 Thermometer Digital Bottle refrigerator freezer 1 Traceable V Humidity temperature dew point meter digital Traceable e Simultaneously displays all three current readings e Sits anywhere in the lab and updates every 30 seconds e Relative humidity range 1 to 99 e Ambient temperature and dew point range 14 to 140 F 10 to 60 C e Resolution 0 1 Cat No Description Pack Oty 15264006 Humidity temperature dew point meter
73. ensus Offset in 0 1 increments offset range 5 C 9 F ACU eh ee en eT E eT DU DEDERE 0 002pH 1LSD Output 225 5252 RS 232 phono plug mini B USB stirrer BUNGE SEES neos idituns USA 2 000 4 010 6 997 10 013 12 000 Language Selection English French Spanish German Italian Chinese Korean NIST 1 678 4 010 6 865 9 184 12 460 EE A O EE E eet ae ee DIN 1 090 3 060 4 650 6 790 9 230 12 750 Memory eese D00 data sets viewable T RR FSCI 1 000 3 000 6 000 8 000 10 000 13 000 Datalogging ees Manual timed selectable every 3 to 3600 seconds Pa eee UON CUSTOM Any 2 5 values 21 0pH unit apart Matt do CoV format Slope Display 5 esses Yes up to 5 different slopes with offset Temp Compensation Automatic or manual 0 to 100 C 32 to 212 F mV Mode Range ro nr OR 2000 0mV Rel mV EESUENME aE 0 1mV PEDE A 0 2mV or 0 05 whichever is greater Offer Adj stm nt dnexacuzitex dicte eimi urea factor diia don Up to 150mV Cat No Description 12840633 AB150 Meter Includes meter electrode arm RS232 amp USB cables 100 240V power supply and manual 12870633 AB150 Kit Includes meter TRIS compatible pH ATC electrode Cat No 11500194 electrode arm RS232 amp USB cables 100 240V power supply and manual 12880633 AB150 BioBasic Kit Includes meter TRIS compatibl
74. er s solution pure for detection and determination of NH3 and salts echiff s reagent pure for determination of aldehydes Leishman s stain pure in 100 Methanol Thymolphthalein pure solution 0 2 indicator grade Reagents for COD Bet at Cat No 10334980 10578420 10518240 10020230 10030230 _ Description Chloride colour reagent pure Ficodox pure mixed COD reagent Potassium dichromate for COD solution 0 021 M 0 125N Silver nitrate for COD solution 1000 g l Silver sulfate for COD solution 5 w v d 1 84 in sulfuric acid www eu fishersci com IE UK EN 2014 200 10187 FOCUS ph electrochem master indd 44 Quantity 500mL 500mL 500mL 500mL 500mL 100mL 500mL 100mL 25b0mL 500mL E 2 5L 500mL 500mL 500mL 500mL 500mL 500mL 500mL 500mL 500mL 2 5L 500mL 500mL 500mL 100mL 500mL 100mL 500mL Quantity IE 1L 100mL 1L le 2 5L ADE 500mL 100mL 100mL 500mL 500mL 100mL 500mL Quantity 4 5L 2 5L Tu 500mL 2 5L For up to date GHS information on Fisher Chemical products listed please refer to the safety data sheet available from www eu fishersci com 08 09 2014 16 20 51 Heagents for Analysis Chemical Cat No Description 10203330 Cetyl trimethylammonium bromide for analysis 10020160 Hydrogen peroxide 6 w v 20 volumes for analysis 10686832 Hydrogen peroxide 6 w v 20 volumes for analysis 10345770 Water fo
75. ere are many reasons for this for example many pH meters require an adapter to accept half cell electrodes handling multiple electrodes is undesirable or impossible with small samples half cells don t have built in ATC and therefore require a third electrode for temperature compensation the complexity of diagnosing electrode problems and most of all the reduced cost but consistent performance of today s combination electrode designs Glass or Plastic Body To decide on which to use let us look at the advantages and disadvantages of each starting with the glass body electrode Glass electrodes are easier to clean and maintain since they can tolerate Just about any solvent and inorganic material with the exception of HF and can adequately handle higher temperatures with the bulb typically able to go up to 100 C The fact that glass electrodes also have a glass sensing bulb is also an advantage Since the seal that combines the bulb to the body is similar material it is one less thing that can go wrong during measurement This Is especially important consideration for applications that involve repeated and extreme heating and cooling the expansion and contraction that occurs is handled much better by glass electrodes The downside of glass electrodes is fairly easy to discern they are generally more expensive then plastic and they have a greater potential for breakage Plastic electrodes are less expensive than glass equivalents an
76. ersci com catalogues www eu fishersci com 08 09 2014 16 14 03 IE UK EN 2014 200 10187 FOCUS ph electrochem master indd 4 FOCUS ON WATER ANALYSIS WORKFLOW 6 7 INTRODUCTION TO ELECTROCHEMISTRY 8 ACCUMET ELECTROCHEMISTRY METERS s 9 e Fisherbrand accumet AB and XL Benchtop Meter Series 10 oelecdon Gulge Tor Benchtop TVIBLBfB ee coratesidunt decere drat aves oda ucc orti eu eerta k 10 accumet AB Series gi ciuile rl MN RR D o Um NONU 11 sd iei T m 11 au iL les NP RR RN 12 accumet XL Series o E ul A E A A EEA AA V A TE T 13 E e aiar EAEE A EE near amie isda E E a etnsse ed aenn te taeateeeeens 13 BM i XX 14 a Re cp ds T PT H tees 14 Duh cip nil T c N 15 e Fisherbrand accumet AP Portable Meter Series sss 16 sselection Guide for Portable Meters seen nnne ne 16 Accumet AP Series UM E EP DA NIO RN o T TR 17 AP74 and AP84 Dissolved Oxygen Meters essent 18 AP75 and AP85 Conductivity Meters usestusesi sides qe sa gutd Gdvas deett reete preci andreas basti mtm 19 van Uber cu m BIS CRT 20 e Probes and Other Accessories for accumet Meters sss 21 ACOUMEU ee PO UI PODE erreren inaina Ena raaraa ER a eA EKAk NAi
77. ffer and calibrate with the correct buffer value at the measured temperature Relationship between pH and Temperature The most common cause of error in pH measurements is temperature Temperature variations can influence pH for the following reasons e he electrode slope will change with variations in temperature e Buffer and sample pH values will change with temperature e Measurement drift can occur when the internal elements of the pH and reference electrodes are reaching thermal equilibrium after a temperature change e When the pH electrode and temperature probe are placed into a sample that varies significantly in temperature the measurements can drift because the temperature response of the pH electrode and temperature probe may not be similar and the sample may not have a uniform temperature so the pH electrode and temperature probe are responding to different environments 100 C 74 mV pH Unit 50 C 64 mV p 0 C 54 mV p Electrode slope changes can be compensated for by using an automatic temperature compensation ATC probe Fisherbrand accumet pH meters calculate the electrode slope based on the measured temperature t of the pH buffers The meter will automatically adjust the pH buffer value to the actual pH of the bufferat the measured temperature E i Isopotential Point The pH values of buffers and samples will change with variations in temperature because of their E temperature dependent chemical equil
78. ft 38 Www eu fishersci com IE UK EN 2014 200 10187 FOCUS ph electrochem master indd 38 08 09 2014 16 18 32 Technical Resources TROUBLESHOOTING GUIDE TechSupport Here are some of the most commonly experienced problems associated with pH and other electrochemical measurements along with some useful suggestions for solving them Problem Cause 05 Suggestions e Short out meter in mV mode by inserting one end of a paper clip in to the centre of the BNC connection touch outer edge with other end Meter should read OmV If you get a different reading meter may be in need of repair Ensure that fresh buffer solutions are always used Rinse electrode between buffer solutions _ Are pH buffers more than 1 0pH unit apart Custom pH Meter will not calibrate calibrations must be more than 1 0pH unit apart Ensure that electrode has been stored correctly refer to pages 37 e Check electrode for cracks scratches etc Clean electrode refer to pages 37 Drain flush and refill electrode Replace electrode 2 Technique Make sure that reading is being given sufficient time to stabilise Check electrode for cracks scratches etc Clean electrode refer to pages 37 e Drain flush and refill electrode ensure fill solution level is high Meter is giving erratic Perie Uncover fill hole during measurement readings or readings are e Shake any air bubbles out
79. g for hand held operation Model 505 Sonic Dismembrator Processes 0 5 to 500mL 500 watts programmable from 1 second to 10 hours overload protection pulse mode 1 second to 59 seconds energy display watts and Joules Model 705 Sonic Dismembrator Processes 0 2 to 1 000mL 700 watts touch screen display programmable from 1 second to 99 hours save up to 10 different programs overload protection pulse mode 1 second to 24 hours energy display real time watts and cumulative Joules temperature monitoring requires optional temperature probe full amplitude control from 1 10096 e Applications include Cell disruption Homogenisation ChIP DNA shearing Nanoparticle dispersion Protein extraction Sonochemistry Soil extraction Mixing Cat No Description 12961151 Model 50 Includes generator converter 3mm probe cables wrench set manual 230V 50 60Hz 203mm x 190mm x 152mm W x L x H 12337338 Model 120 Includes generator converter 3mm probe cables wrench set manual 230V 50 60Hz 203mm x 330mm x 152mm W x L x H 12893543 Model 505 Includes generator converter 13mm probe cables wrench set manual 230V 50 60Hz 203mm x 387mm x 216mm W x L x H 12931151 Model 705 Includes generator converter 13mm probe cables wrench set manual 230V 50 60Hz 203mm x 387mm x 216mm W x L x H Accessories Cat No Description 12991181 Cup horn for indirect processing of individual sealed tubes or
80. h screen for easy operation Technical Specification Temperature Mode pH Heiton agespteridicieaedediab vb teda o ose tesh 5 0 to 105 0 C Ae er ec aren nate er se eee ent 2 000 to 20 000pH PO Mme 0 1 C 0 1 F ucc Pom 0 1 0 01 0 001 pH W i Tm 0 2 C 0 3 F POGINGCY ieueumitusieiou mas Mied 0 1 0 01 0 002 1LSD Output RJ45 RS232 USB A mini B USB two stirrer ports Buffer Sets 2222222 USA 2 00 4 01 7 00 10 01 12 00 Language Selection English French Spanish German Italian Chinese suse eonasaein tas NIST 1 68 4 01 6 86 9 18 12 46 QM an EO s MH a DIN 19267 1 09 3 06 4 65 6 79 9 23 12 75 Datalogging eese 2000 reading datalog capability FSCI 1 00 3 00 6 00 8 00 10 00 13 00 OPNE IEE NE IEEE Pure Water 4 10 6 97 9 15 oe CUSTOM 2 5 points any values 21 0pH units apart mV Mode HUN h Siieuteastsidbouuaund ectcendis 2000mV Rel mV POCO Ausedaucdarautiusuiim nias Era UN OMEN 0 1 20000 cR 0 2 CatNo Description 12890633 XL150 Meter Includes meter electrode arm RS 232 amp USB cables 100 240V power supply and manual XL150 Kit Includes meter TRIS compatible accuTupH pH electrode Cat No 11550174 ATC probe Cat No 10236064 electrode arm RS232 amp USB cables 12820643 110 220V power supply and manual oH Meter XL200 No need to swap probes measure and display two channels si
81. hem master indd 13 08 09 2014 16 14 54 14 pH Meter XL 250 Multiple BNC ports allow flexibility Technical Specification lon Mode pH HOME uskascsiteeiieds ofi iei ei ROM dS 1x10 103 99 x 10 I M S PE A E 2 000 to 20 000pH biz 9 10 M 0 1 0 01 0 001 Resolution M gunebtditEdf anra AE 0 1 0 01 0 001 pH POC aane a nerd 0 5 full scale monovalent ion BEOHIBUN dausendiubeiiinee es cee tease 0 1 001 0 002 1L5D 5 dldumesudsuenuse e aas 196 full scale divalent ion Auto Buffer Recognition 5 preset buffer groups custom Output ee RJ45 RS232 USB A mini B USB two stirrer ports mV Mode Language Selection _ English French Spanish German Italian Chinese ON ee Ze MEIN 5 5 5 7 O gi sin dubias iaa mot Pet Korean amp Portuguese 5 tio MEM 0 1 Cu c rer THR CN 31 2 Temperature Mode Resolution esses 5 0 to 105 0 C POG M 0 1 C 01 F Calibration O O aE E fitted 0 2 C 0 3 F CatNo Description 12850643 X1250 Meter Includes meter electrode arm RS 232 amp USB cables 100 240V power supply and manual 12860643 XL250 Kit Includes meter TRIS compatible accuTupH pH electrode Cat No 11550174 ATC probe Cat No 10236064 electrode arm RS232 amp USB cables 110 220V power supply and manual 12870643 X250 Fluoride Kit Includes meter fluoride electrod
82. hersci com IE UK EN 2014 200 10187 FOCUS ph electrochem master indd 32 08 09 2014 16 16 29 Technical Resources pH Electrode Calibration You Will need pH meter Electrode Wash bottles pH buffers pages 10 to 20 pages 27 pages 31 pages 29 to 30 pages 45 E Beaker otirrer Magnetic follower accumet stirrer probe SEL RR pages 47 pages 58 pages 58 pages 21 Calibration Recommendations e Always pour fresh pH buffers into clean beakers for calibration Choose buffers that are one to three pH units apart e Check the electrode slope daily by performing at least a two buffer calibration The slope should be 92 to 102 54 43 to 60 34 mV per pH unit e f the electrode is refillable uncover the fill hole during calibration to ensure a uniform flow of filling solution The filling solution level inside of the electrode must be at least one inch above the buffer solution level e he buffer solution level must be above the pH electrode reference junction when the electrode is immersed in the buffer e Between buffers rinse the electrode with distilled water and then with the next buffer To reduce the chance of error due to polarisation avoid rubbing or wiping the electrode bulb Use a lint free tissue and gently blot the bulb e Use a magnetic stir plate and stir bar or the accumet benchtop stirring probe to stir all buffers and samples at a moderate uniform rate The stirrer probe can be used with the AB and XL series
83. ibria The pH electrode should be calibrated with buffers that have known pH values at different temperatures Since pH meters are unable to correct sample pH values to a reference temperature due to the unique pH versus temperature relationship of each sample the calibration and measurements should be performed at the same temperature and sample pH values should be recorded ii with the sample temperature 0 7 14 pH Hg pH oe 5C 0c 200c 30 C 40 C 50 C 60 C 70 C 80 C 9C Value at25 C 1 68 1 87 Ta 167 167 1 68 1 69 S me m Tw 179 40 4 00 4 00 4 00 4 00 402 404 4 06 4 09 A 416 421 6 86 6 98 6 95 692 6 87 6 85 6 84 6 83 6 84 6 85 6 86 6 88 1 00 21 7 08 1 06 101 6 98 6 87 697 6 97 6 89 7 03 708 9 18 9 46 9 40 9 33 9 23 914 9 07 90 8 96 8 92 8 89 8 85 10 01 10 32 10 25 10 18 10 06 9 97 9 89 9 83 979 9 78 9 78 9 80 12 46 12 79 128 Pa Dg 1236 Wy 11 96 le M47 Lie 10 89 www eu fishersci com 08 09 2014 16 15 57 pH Electrodes Electrode Selection When selecting a pH measurement system choose your meter based on what features you need e g resolution output memory portability etc Choose the corresponding pH electrode for your meter based primarily on your sample type and conditions e g wastewater with sulfides room temperature student usage five days a week etc While many electrodes might work adequately for a particular application not all will perform equally or last as long
84. icator paper reel pH 11547392 pH indicator paper reel pH 11557392 pH indicator paper reel pH 11567392 pH indicator paper reel pH 10433151 pH indicator paper reel pH three colour Wash Bottles HOPE e Labelled and colour coded for most commonly used solvents e High density polyethylene e 500mL capacity Cat No Label 11745233 Acetone 11755233 Bleach 11765233 Deionised water 11775233 Distilled water 11785233 Ethanol 11795233 Isopropanol 11705243 Methanol 11715243 Saline 11725243 Soap 11735243 Universal 11745243 Water Solvent 11765243 Cleaning Colour change Red to blue Blue to red pH specific pH specific pH specific pH specific pH specific pH specific pH specific pH specific pH specific You may also be interested in the following pH accessories pH range Ue tet pH range 4 0 10 0 4 0 10 0 4 0 10 0 Colour change colour chart on bottle 1 0 13 0 colour chart on bottle Colour change colour chart on bottle colour chart on bottle colour chart on bottle pH range 0 0to 14 0 0 0 to 6 0 17 103 8 100 sticks 4 5 to 10 0 6 0 to 7 7 7 0 to 14 0 3 6 to 6 1 pH range 5 0t08 0 5 0t08 0 1 0 to 14 0 1 0 to 11 0 OS mS 4 0to7 0 6 4 to 8 0 8 0 to 10 0 9 0to 13 0 2 0 to 14 0 1 0 to 11 0 e Translucent with coloured closure e Comes with vented 38mm closure to prevent solvent drips Closure colour Red
85. in de ionised water e Never stir the sample or buffers with the electrode e Never cover the reference fill hole during measurement e Regularly change the reference fill solution IE UK EN 2014 200 10187 FOCUS ph electrochem master indd 42 www eu fishersci com 08 09 2014 16 20 51 IE UK EN 2014 200 10187 FOCUS ph electrochem master indd 43 Technical Resources Conductivity Q am looking to measure pure water samples Is this possible A This is possible What s important is the conductivity cell constant also known as the K value A cell constant of 0 1 would be needed for pure water samples Each cell constant has a limited sensing range so please make sure you choose one whose range encompasses that of your expected sample conductivity See below for examples of sample types approximate conductivity values and suitable cell constants y S cm sample mS cm sample 0 1 1 0 10 100 10 100 1000 E deionised water boiler ipod K Pure water 0 055 Tap water 50 Epi water Seawater 53000 waste water 59 NaOH 223 000 surface wae eS LLL EE sea water process water conc acids and lyes Sample Conductivity uS K 20 6 and 0 475 Ls K 100 0001M 001M 01M AM 14 940S 147 008 1 413mS 12 9mS Q Can mix and match meters and conductivity cells from different manufacturers A There is currently no standard connection for meters and conductivity
86. ing the electrodes lifespan Therefore whilst these are generally the most expensive electrodes they will need replacing much less frequently in difficult sample types The sleeve junction electrodes are also TRIS compatible pH Electrode Features Em EI Size m 8 e Body E s ee ad pem drilled 12mm electrode diameter y Compatible with virtually Hole Open for use in a wide variety any sample including Best junction for dirty of sample sizes Solvents difficult samples Easy to clean ee Junction is clog free and nu N 4 F Plastic Body IN Ld dimer los ei Extremely durable Ideal for thick or viscous LE Miu and rugged to prevent samples compatible with nents breakage Moo all sample types Micro Value priced Ceramic and Glass 2 a s 2 rie Capillary bonu ll ad Better junction for routine a as small as 0 5L and a ctu containers as smallas ail Junction is high quality ls JL NND RE and durable TUITSHD deal for most applications When glass is required and samples the BS Jurahle pH bulb oh W bed e Tm Fibre I semi solid samples and measuring small volume samples Flat Surface Tip For measuring surfaces of li solid or gel samples and measuring small volume samples plastic electrodes Ideal for aqueous samples Spear n labor field use END V t e Junct
87. ion used with rugged 20 www eu fishersci com IE UK EN 2014 200 10187 FOCUS ph electrochem master indd 25 08 09 2014 16 16 11 pH Electrodes Use the selection guide below to help you choose the the right Fisherbrand electrode for your particular application pH Electrode Selection Guide Sample Application Type General Purpose for many common sample types e g aqueous samples drinking water etc Food and drink e g juices baby food cheese Environmental samples e g wastewater soils sea water Low ionic strength e g treated effluent deionised water distilled water Non aqueous e g solvents alcohols Life science samples Iris containing reagents proteins etc Small samples e g samples stored in test tubes small flasks and beakers High viscosity samples e g slurries suspended solids pH Electrode Connectors Recommended Electrode Type ne E EU Glass bodied URN Plastic bodied up NU UNE CURE Tuff Tip Ad peti nett ed oi bib cs T DIU pet Spear tip m Tu Hi p A E E E TE Sleeve junction EE EE TATE Tuff Ti p Thin stem Semi micro HOT EE HE TAT EE Sleeve junction Cat No 11748788 11706358 11755638 11736208 11755638 11726358 11755638 11769798 11726358 The type of pH electrode connector can vary according the brand of the meter as well as the electrode type The following information provides you with a brief overview of these con
88. ket for all AB and XL series benchtop meters i 12800663 Optical USB mouse Compatible with any XL series ab 12850653 Replacement power supply for AB150 AB200 AB250 XL150 XL200 XL250 XL500 EN XL600 100 240 VAC i 12870653 Replacement RS 232 output cable for PC and printer npe 12880653 To download your free communication software for your AB and XL accumet meters visit www eu fishersci com fisherbrand ae www eu fishersci com IE UK EN 2014 200 10187 FOCUS ph electrochem master indd 22 08 09 2014 16 15 56 IE UK EN 2014 200 10187 FOCUS ph electrochem master indd 23 pH Electrodes pH ELECTRODES pH Electrode Theory pH electrode measurements are made by comparing the readings in a sample with the readings in standards whose pH has been defined i e standard buffers When a pH sensing electrode comes in contact with a sample a potential develops across the sensing membrane surface that varies with pH A reference electrode provides a second unvarying potential to quantitatively compare the changes of the sensing membrane potential Combination pH electrodes are composed of a sensing electrode with the reference electrode built into the same electrode body Combination electrodes provide the same selectivity and response as a half cell system but offer the convenience of working and maintaining only one electrode A meter serves as the readout device and calculates the difference between the reference elec
89. l scale r 5 Lone NOE XU 0 00uS to 500 0mS Datalogging Manual timed selectable every 3 to 3600 seconds Resolution Cece hereon 0 01 0 105 0 001 0 01 0 1mS j gs rm Printer or CSV format Pe Asia ust s ise aa camera aa 1 full scale Coefficient Per C 22s Linear amp pure adjustable 0 000 to 10 00096 Normalization Capea enraptured end doma d Ud 15 0 to 30 0 C Cat No Description 12800643 AB200 Meter Includes meter electrode arm RS232 amp USB cables 100 240V power supply and manual 12810643 AB200Kit Includes meter TRIS compatible pH ATC electrode Cat No 11500194 Conductivity Temp probe Cat No 11550164 electrode arm RS232 amp USB cables 100 240V power supply and manual 11 www eu fishersci com IE UK EN 2014 200 10187 FOCUS ph electrochem master indd 11 08 09 2014 16 14 48 pH Meter AB250 Versatile BNC connection for pH redox ORP or ion selective measurements benchtop Meters Technical Specification f rr UN ppm mg L molar pH PENNA teh at T aeae vanes aap dues 0 5 full scale monovalent ion ee a ae ee SALON coe e 00 0 5 0 O rrr rcp 1 full scale divalent ion 1 1 5 E ec 0 1 0 01 0 001 pH Cal Points denvideneiiisimies 2 to 6 points from one of following groups eil e AHORRAS D seems toS et 0 001 0 01 0 1 1 10 100 Buffer SETS oacpspesnenmeoton USA 2000 42010 DUSZ 10 013 12 000
90. le values 0 1 0 5 1 b 10 50 100 500 1 000 Calibration points from 2 to 5 Range C 5 to 100 5 to 100 5 to 100 Resolution C 0 1 0 1 0 1 Temperature Accuracy C 0 3 0 3 0 3 ipavon temperare Calibration points 1 ATC and 1 MTC Available values 1 point 5 adjustment ATC 5 to 100 default adjustment MTC General Clock Yes date time and GLP Cat No Description 11580184 AP110 Meter Includes meter 9V battery and manual 11590184 AP110 Kit Includes meter pH ATC electrode Cat No 11560194 refill solution hard carrying case sample bottles pH calibration packets 9V battery and manual 11560184 AP115 Meter Includes meter 9V battery and manual 41570184 AP115 Kit Includes meter pH ATC electrode Cat No 11560194 refill solution hard carrying case sample bottles pH calibration packets 9V battery and manual 11540184 AP125 Meter Includes meter 9V battery and manual AP125 Kit Includes meter pH ATC electrode Cat No 11560194 refill solution hard carrying case sample bottles pH calibration packets 9V battery 11550184 and manual Replacement Parts and Accessories for AP100 Series Portable Meters Cat No Description 11560194 pH ATC electrode refillable single junction epoxy body for AP100 series portable meters Refer to pages 21 to 22 for further information on the range of probes and accessories for accumet meters 20 IE UK EN 2014 200 10187 FOCUS
91. lectrochemistry Fisherbrand f Sample Storage Sample Transportation Bags Carriers Bottles Trays Freezers Refrigerators Thermometers Thermometers i ee c 25 255 52 c22222 2 555 Unee 0000 0Q0 nH o 0 OO OS o m p Eme ee e et portable meters on accum refer to cus ddl usd 9 e Handheld pH meters e Handheld conductivity meters e Handheld dissolved oxygen meters e Electrodes e pH buffers e Conductivity standards e pH indicator sticks reels sow 3 Pr che Oducts featured also refer 1 a m Wwww eu fishersci com IE UK EN 2014 200 10187 FOCUS ph electrochem master indd 7 Fisher Chemical Sampling Bags Beakers Bottles Cylinders Funnels Petri dishes e e e s s 0 0 2 0 0c 00 220 0 0 2 s o c f Fisher BioReagents 08 09 2014 16 14 07 IE UK EN 2014 200 10187 FOCUS ph electrochem master indd 8 Flectrochemistry INTRODUCTION TO ELECTROCHEMISTRY Next to temperature and mass pH is the third most common laboratory measurement It crosses over many disciplines from water wastewater analysis basic research and development environmental chemical and life sciences and an endless number of industrial applications Below are some examples of pH in a few common industrial and household products Household dye 13 6 13 1 Bottle washing Copper plating 12 8 12 6 Bleach Ammonia 11 4 TI 11 3 Brass plating Milk of magnesia
92. lvents All chemicals are ISO 9001 2008 certified and undergo rigorous quality assurance and testing procedures ensuring excellent lot to lot and bottle to bottle consistency Supported by a clear and simple grade and application structure choosing the product that best suits your requirements is easy e Fisher Bioreagents offers over 1 000 products dedicated to molecular biology research biochemistry and cellular biology It is your single source for high purity products Fisherbrand Chewwteal f Fisher BioReagents Together Fisherbrand Fisher Chemical and Fisher Bioreagents offer reliable and essential laboratory products helping you to produce your best work each and every day New products are constantly being introduced into the Fisherbrand family For the full range visit www eu fishersci com fisherbrand www eu fishersci com IE UK EN 2014 200 10187 FOCUS ph electrochem master indd 2 08 09 2014 16 11 46 General Introduction IE UK EN 2014 200 10187 FOCUS ph electrochem master indd 3 This brochure is dedicated to providing you with a comprehensive iil d i ectrochemistry overview of our pH and electrochemistry portfolio as well as highlighting Video todisDybe mr supplementary products from the Fisherbrand family Featuring a range of instruments consumables and Fisher Chemical as well as useful product resources such as selection guides troubleshooting guides FAQ s and workflows it is a great lab companion
93. lysis tubing 6 37mm diameter 15m roll 12 to 14kDa MWCO i 15293847 Dialysis tubing 15 9mm diameter 15m roll 12 to 14kDa MWCO i 15253857 Dialysis tubing 28 6mm diameter 15m roll 12 to 14kDa MWCO a 15283847 Dialysis tubing 6 37mm diameter 30m roll 12 to 14kDa MWCO 1 15273847 Dialysis tubing 15 9mm diameter 30m roll 12 to 14kDa MWCO 15263847 Dialysis tubing 21 0mmM diameter 30m roll 12 to 14kDa MWCO 1 15253847 Dialysis tubing 28 6mm diameter 30m roll 12 to 14kDa MWCO 1 15233847 Dialysis tubing 12 1mm diameter 15m roll 3 500Da MWCO i 15263857 Dialysis tubing 29 3mm diameter 15m roll 3 500Da MWCO i 15273857 Dialysis tubing clamp sealing width up to 46mm 10 15243847 Dialysis tubing clamp sealing width up to 65mm 10 Dispenser bottle too e Excellent chemical compatibility e Springless precision valve mechanism e Fully autoclavable e Anti drip cap e Easy to calibrate e Fits on most common sizes of bottle e Removable piston e Traceability to an NABL accredited laboratory Cat No Capacity mL Pack qty 12867913 D25025 each 12877913 05t05 each 12887913 1to 10 each 12897913 25 to 30 each 12807923 5 to 60 each 12817923 10 to 100 each Pipette tillers universal NEW e The side mounted thumbwheel is effortlessly manipulated for exact aspiration or drop wise delivery e The plunger is easily depressed for quick dispensing e Colour coded barrel indicates pipette size Cat No Description Pack qty 1520
94. mL 10457711 Buffer solution pH4 00 phthalate stabilised with 10ppm mercuric chloride standard buffer solution 10164863 Buffer solution pH4 00 phthalate ampoule colour coded stabilised with 10ppm mercuric chloride volumetric analysis solutrate Fisher OHS Buffers Chemical Cat No Description 10609483 Buffer standard solution pH5 00 phthalate stabilised with 10ppm mercury II chloride traceable to NIST 10588230 Buffer standard solution pH5 00 phthalate stabilised with 10ppm mercury II chloride traceable to NIST 10294430 Buffer standard solution pH5 00 phthalate stabilised with 10ppm mercury Il chloride traceable to NIST Quantity mL 500 1000 2 500 5 000 10 000 100 6x100 500 1000 2 500 5 000 10 000 6x100 500 1000 2 500 5 000 10 000 100 6x100 Quantity mL 500 1000 2 500 100 6x100 Quantity mL 500 1 000 2 500 Quantity mL 50 tablets 500 1000 2 500 5 000 10 000 6x500 1 000 100 500 100 Quantity mL 500 1 000 Z S00 For up to date GHS information on Fisher Chemical products listed please refer to the safety data sheet available from www eu fishersci com www eu fishersci com IE UK EN 2014 200 10187 FOCUS ph electrochem master indd 29 08 09 2014 16 16 14 pH Butters 23 pH Buffers 30 Fisher OH6 Buffers Chemical Cat No Description 10690404 Buffer standard solution
95. ming indicating elapsed time since alarming e Timing range 99 minutes 59 seconds e Resolution 0 1 e Accuracy is 0 01 Cat No Description Pack qty 15204016 Timer extra extra large digit Traceable 1 Timer multicoloured Iraceable M e Counts down and alarms on 4 separate channels e Counts up during alarming indicating elapsed time since alarming e Times up to 99hr 59min 59s e Accuracy 0 01 e Resolution 1 second e Dimensions mm 70 x 57 x 13 Cat No Description Pack Oty 11775863 Timer multicoloured Traceable i Timer three channel alarm Traceable M e Run three channels at same time e Alarm volume for high decibel to low decibel e Functions as a stopwatch and a clock e Timing range 99 hours 59 minutes 59 seconds e Resolution 1 second e Accuracy 0 001 e Dimensions mm 83 x 76 x 35 4 Cat No Description Pack qty 11725863 Timer three channel alarm Traceable M 1 Timer four channel alarm Iraceable M e Extra loud high decibel alarm e Features include stopwatch time out and a clock e Memory returns to previously programmed time e Timing range 99 hours 59 minutes 59 seconds e Resolution 1 second e Accuracy 0 01 e Dimensions mm 70 x 63 5 x 13 Cat No Description Pack qty 11745863 Timer four channel alarm Traceable Fisherbrand Supplement CRI The ELLE www eu fishersci com IE UK EN 2014 200 10187 FOCUS ph electrochem master indd 67
96. multaneously Technical Specification Conductivity Mode pH 1 AMEN eee 5 0 to 105 0 C ENS Auuseiitibetisesdqnbidi toni ONES 2 000 to 20 000pH Resolution aeseibtsip eot nt E reto eb vt etta 16 0 TF Resolution ddediesebedstpn ie diiit dei 0 1 0 01 0 001pH ich MEME ep 0 2 C 0 3 F Accuracy iseuestixsiondddiaiibtni eren 30 1 0 01 0 002 1LSD Output gt RJ45 RS232 USB A mini B USB two stirrer ports Auto Buffer Recognition sss 5 preset buffer groups custom Language Selection English French Spanish German Italian Chinese MV Mode 2 p 9 p Q n o 0 00000000 nasseneeeInememeememnrenmenrnrnr nennen Korean amp Portuguese 3 E LL nnt 2000mV Rel mV POCONO 5 dusuionHeidid Hali M nob RE MONEO Mtt da aa 0 1 P dii Mer 0 2 Temperature Mode 2 90 Nee Te 5 0 to 105 0 C RM C a ea enced ee MEER OCF Calibration ee eee ce eet ere eae eee E 0 2 C 0 3 F CatNo Description 12830643 XL200 Meter Includes meter electrode arm RS 232 amp USB cables 100 240V power supply and manual 12840643 XL200 Kit Includes meter TRIS compatible accuTupH pH electrode Cat No 11550174 ATC probe Cat No 10236064 Conductivity Temp probe Cat No 11550164 electrode arm RS232 amp USB cables 100 240V power supply and manual www eu fishersci com IE UK EN 2014 200 10187 FOCUS ph electroc
97. nectors However if you require any further assistance connecting your electrode to your meter contact our Product Support Advisors e The BNC connector is accepted by the vast majority of modern pH meters It is characterised by its locking twist action e The S7 screw cap connectors are becoming more popular due to their flexibility Electrodes with an S7 head can be connected to a variety of meter inputs using a separate cable Vl e The DIN connector although not as common as a BNC is still used by several different meter types Refer to Electrode Accessories page 27 for the Fisherbrand electrode adapter cables which will allow you to connect probes from alternative manufacturers to your accumet pH meter 26 IE UK EN 2014 200 10187 FOCUS ph electrochem master indd 26 Www eu fishersci com 08 09 2014 16 16 11 EN 11706358 11776348 pH Electrodes Cat No Electrolyte 11706358 Gel 11776348 Gel 11786348 Gel 11749798 KCL AM AgCI 117397988 KCLAM AgCI 11786338 KCL AM AgCI 11726358 KCL 3M AgCI 11769798 KCL 4M AgCI 11709818 KCL 4M AgCI 11736209 Gel 11755638 Gel 11765638 Gel 11775638 Gel 11785638 Gel 11749798 11739798 Style Sleeve junction Micro electrode Micro electrode Spear tip Tuff Tip Tuff Tip Tuff Tip double junction Tuff Tip double junction Tuff Tip is a robust alternative to conventional laboratory pH electro
98. ngth x 50mm diameter 1 12927106 Straight blade impeller 400mm length x 50mm diameter 1 12937106 Pivoting blade impeller 400mm length x 60mm diameter 1 12947106 Collapsible blade impeller 400mm length x 90mm diameter 1 11920305 Square blade impeller 400mm x 70mm square 1 11900305 Pitched blade propeller 400mm x 64mm diameter 1 11984985 Compact stand with base 457mm x 8mm for 15203807 only i 12987116 Pitched blade propeller 25mm diameter x 8mm bore 1 12997116 Pitched blade propeller 38mm diameter x 8mm bore 1 12907136 shaft 300mm length x 8mm diameter 1 12917136 Shaft 300mm length x 9 5mm diameter 4 12927136 Shaft 457mm length x 8mm diameter 1 12937136 Shaft 457mm length x 9 5mm diameter 1 12947136 Shaft 508mm length x 9 5mm diameter i 12957136 Shaft 610mm length x 8mm diameter 1 12967136 Shaft 610mm length x 9 5mm diameter i 12977136 Shaft 762mm length x 9 5mm diameter 1 12997136 Vinyl chuck cover for 15203807 only i 99 08 09 2014 16 22 06 60 sonic dismembrator e Four models available Model 50 Sonic Dismembrator Processes 0 2 to 50mL 50 watts continuous or remote operation footswitch optional lightweight converter 340g for hand held operation Model 120 Sonic Dismembrator Processes 0 2 to 50mL 120 watts programmable from 1 second to 10 hours overload protection pulse mode 1 second to 59 seconds energy display watts and Joules lightweight converter 340
99. nimum two point calibration The table below provides pH value for buffers at various temperatures Bui 7 5C Qo XeC o 030 AyC 50 C 60 C 70 C 80 C 90 C Valueat25 C 1 68 1 87 1 87 1 67 1 87 1 68 1 69 K e TEA dg 179 4 01 4 00 4 00 4 00 4 00 402 4 04 4 06 409 413 416 A2 6 86 6 98 B 95 6 92 e 6 85 6 84 6 83 6 84 6 85 6 86 6 88 7 00 241 7 08 7 06 701 6 98 697 697 6 97 6 99 7 03 7 08 918 9 46 940 O98 0179 1914 S107 9 01 8 96 8 92 8 89 8 85 10 01 1032 1025 10 18 10 06 997 989 983 948 978 9 78 9 80 12 46 12 79 1206 12 87 m 12 36 SEXE 11 96 TEE ey Ag 10 89 34 TechSupport For further information on any of the featured products contact our Product Support team Www eu fishersci com 08 09 2014 16 17 47 IE UK EN 2014 200 10187 FOCUS ph electrochem master indd 34 IE UK EN 2014 200 10187 FOCUS ph electrochem master indd 35 pH Electrode Measurements TechSupport You will need pH meter Electrode Wash bottles pH buffers E er pages 10 to 20 pages 27 pages 31 pages 29 to 30 pages 45 i 7 a Beaker stirrer Magnetic follower accumet stirrer probe pages 47 pages 58 pages 58 pages 21 Sample Requirements Electrodes with an plastic body should only be used in aqueous solutions e Electrodes with a glass body may be used in nonaqueous solutions and solutions that contain organic solvents A minimum of 2096 water mu
100. nown pH value allow calibration of the meter electrode system to ensure that accurate measurements are taken subsequently Certified accurate buffers are available from Fisher Chemical as ready to use colour coded solutions concentrated solutions capsules and prepackaged salts All have the special characteristic of resisting pH change in the event of dilution or acid base contamination It is generally recommended to use a minimum of two point standardisation first with a buffer value close to the electrode systems zero potential typically pH 7 and also with an additional acid or base buffer whose values encompasses the expected pH value of the sample refer to pH Electrode Calibration pages 33 to 34 For best accuracy the calibration should be performed with ATC at the same temperature as the expected samples In this section you will discover that Fisher Chemical have all the standard buffers you need Manufactured to exacting standards and packaged for convenience you ll find a buffer to meet virtually every laboratory or field application ndn y ETIN eb f ee eb IE UK EN 2014 200 10187 FOCUS ph electrochem master indd 28 Her fL Lok pa Unit s aian pid 10 Ferri Trebia we IST E der LL Sd o 1 mj Se m Be c HN MN oc n c s RET EH e og de www eu fishersci com 08 09 2014 16 16 14 Fisher Colour Coded Buffer Solutions Chemical Cat No Description 10457711 Buffer colour coded solution pH4
101. ns Height 0D mm Pack qty mL mL mm 11759398 100 10 72 58 100 11769398 250 10 90 76 100 11799398 100 50 M5 15 100 Www eu fishersci com IE UK EN 2014 200 10187 FOCUS ph electrochem master indd 48 08 09 2014 16 21 00 Bottles narrow mouth HDPE Cat No 11735383 11745383 11765383 11775383 11785383 11907974 11937964 11997954 11977924 Bottles wide mouth HDPE Cat No 11775243 11785243 11795253 11775253 11745253 11917974 11947964 11907964 11987924 Bottles wide mouth HDPE amber Cat No 11927914 11967944 11977944 11957924 11937914 Capacity mL 30 60 250 500 1 000 125 250 500 1 000 Capacity mL 30 60 250 500 1 000 125 250 500 1 000 Capacity mL 60 125 250 500 1 000 0 D mm 38x 84 48x99 P 71x171 91 x 213 48 x 99 61x 132 XI Ok Zils 0 D mm 36 x 64 38x 86 62 d 74x 168 81 x 201 91 x 99 572 3 18 74x 168 81 x 201 0 D mm 36x 64 38 x 86 Top 74x 168 81 x 201 Bottles wide mouth PP Cat No 11917934 11927934 11937934 11947934 11927924 11957904 Bottles wide mouth LOPE Cat No 11957934 11967934 11977934 11987934 11937924 11967904 Capacity mL 30 60 25 250 500 1 000 Capacity mL 30 60 125 250 500 1 000 0 D mm 36 x 64 38 x 86 51x99 62x132 74x168 81x201 0 D mm 36x 64 38x 86 51x99 62x
102. o Description Pack qty 11862710 Counter Pen i Pens permanent marker e Produce precise lines of 0 3mm width e Perfect marking pens for lab samples microscope slides photographic slides glass and plastic beakers and plastic sample bags e Writes on all surfaces including glass film wood plastic metal rubber and porcelain e Instant writing long storage time and dry out prevention are assured by an airtight ring seal cap e Permanent ink dries in a split second and is waterproof and fade proof Cat No Colour Pack qty 11734386 6 black 2 each red blue green ap Jags aluminium e For use in hot cold and moist environments write on with ballpoint pen write on both sides e 79 4mm x 35mm Cat No Description Pack qty 11764376 Tags aluminium 48 Fisher Scientific Meet the Fisher Scientific Family Fisherbrand Fisher Chemical and Fisher Bioreagents fit everywhere in your lab In the hood In the freezer On the bench On the shelves In the field Fisherbrand fishetbiana ls BEL auaunr Rauanure Vaut S ind has a solutio Low Temperature Isotemp Ultra m Freezers 50 C to 86 C For the full range visit Fisherbrand www eu fishersci com fisherbrand isner e Qua ity RELIABILITY VALUE Chemical Fisher BioReagents a Www eu fishersci com IE UK EN 2014 200 10187 FOCUS ph electrochem master indd 52 08 09 2014 16 21 14 SAMPLE PREPARATION Mini centrifuge
103. ock displays AM PM or 24 hour clock at the touch of a button e Accuracy 0 0196 e Temperature range 0 0 C to 50 C 32 F to 122 F accuracy 1 C resolution 0 1 C e Humidity range 20 to 90 resolution 1 accuracy 4 Cat No Description Pack qty 11725843 Thermometer with clock and humidity monitor d Humidity temperature meter memory Iraceable M e 15 8mm high digits e Reads F C e Chemical and shock resistant ABS plastic housing e Dual recall memories e Includes wall mount bench stand fast response solid state sensors and two AAA batteries Cat No Description Pack qty 11765843 Humidity temperature meter memory Traceable V 1 www eu fishersci com IE UK EN 2014 200 10187 FOCUS ph electrochem master indd 63 Fisherbrand Supplement 63 08 09 2014 16 22 28 Thermometer big digit Iraceable M e View from up to 25 feet e External probe sensor and internal ambient sensor e Maximum and minimum temperature memory e External probe range is 58 0 to 158 0 F 50 0 to 70 0 C e Ambient sensor inside case range is 23 0 to 122 0 F 5 0 to 50 0 C e Resolution 0 1 C e Accuracy 1 C e Dimensions mm 70 x 108 x 19 Cat No Description Pack qty 11759755 Thermometer Big Digit Traceable 1 Thermometer digital refrigerator freezer Iraceable M e Range is 58 to 158 F and 50 to 70 C e Resolution of 1 e Accuracy of 1 C e Dimensions mm 70 x 108 x 19 Cat No
104. of benchtop meters e Place a piece of insulating material such as cardboard between the magnetic stir plate and beaker to prevent measurement errors from the transfer of heat to the sample e Handle the micro pH electrodes with care Do not touch the pH bulb and stem against the bottom or walls of the sample containers Calibration Procedure Two or more buffers This procedure is recommended for precise measurements 1 Verify that the pH electrode was prepared correctly and connect the electrode to the meter 2 Select two pH buffers that bracket the expected sample pH The first buffer should be near the electrode isopotential point pH 7 and the second buffer should be near the expected sample pH pH 4 or pH 10 The pH buffers should be at same temperature as the sample If the buffers and samples are at varying temperatures temperature compensation is recommended 3 Rinse the electrode with distilled water and blot it dry with a lint free tissue Place the electrode into the first buffer When the reading is stable set the meter to the pH value of the first buffer at the measured temperature Refer to the meter instruction manual for a detailed procedure The table on page 34 provides pH values for buffers at various temperatures Rinse the electrode with distilled water and blot it dry with a lint free tissue Place the electrode into the second buffer When the reading is stable set the meter to the pH value of the second buffer at
105. of electrode not stable Is electrode tip cracked If so replace electrode Electrodes will typically last 6 12 months If beyond this the electrode may need replacing Technique 1 _ Make sure that reading is being given sufficient time to stabilise Electrode Electrodes are typically lasting lt 6 months when being used with Tris buffers protein samples 2 For Tris buffer and protein samples a double junction electrode Electrode is typically needed Refer to pH Electrode Selection Guide page 26 for further advice New electrode has he fill solution has crystallised around the electrode This is arrived with white Electrode harmless and will not alter performance It just needs to be crystalline build up wiped away or simply rinsed off WIRT TT TU te stipe msi EE E ul c oe l Electrode cracked and leaking page 26 for further advice Instrument reads Calibrate using water bath or known accurate thermometer o ATC Probe Inaccurate temperature Faulty thermistor repair or replace i u u NE s sample being measured at constant room temperature If not then ATC automatic temperature compensation probe may be Temperature useful fthis is not possible then efforts should be made to make measurements at a consistent temperature e g 25 C Other potential problems Ensure operator IS properly trained In measurement te
106. on 1M 1N free from carbonate ready to use Sodium hydroxide solution 1M 1N free from carbonate ready to use Sodium hydroxide concentrated solution 0 1M 0 1 N ampoule Sodium hydroxide concentrated solution 0 5M 0 5N ampoule Sodium hydroxide concentrated solution 1M 1N ampoule Sodium nitrite solution 0 5M 0 5N ready to use Sodium thiosulfate concentrated solution 2 5M to produce 0 5M 0 5N ampoule Sodium thiosulfate solution 0 1M 0 1N ready to use Sodium thiosulfate solution 0 1M 0 1N ready to use Sodium thiosulfate solution 0 1M 0 1N ready to use Sodium thiosulfate solution 0 1M 0 1N ready to use Sodium thiosulfate concentrated solution 0 1M 0 1N ampoule Sulfuric acid solution 0 01M 0 02N ready to use Sulfuric acid solution 0 01M 0 02N ready to use Sulfuric acid solution 0 05M 0 1N ready to use Sulfuric acid solution 0 05M 0 1N ready to use Sulfuric acid solution 0 05M 0 1N ready to use Sulfuric acid solution 0 1M 0 2N ready to use Sulfuric acid solution 0 1M 0 2N ready to use Sulfuric acid solution 0 1M 0 2N ready to use Sulfuric acid solution 0 25M 0 5N ready to use Sulfuric acid solution 0 25M 0 5N ready to use Sulfuric acid solution 0 5M 1N ready to use Sulfuric acid solution 0 5M 1N ready to use Sulfuric acid solution 0 5M 1N ready to use Sulfuric acid solution 0 5M 1N ready to use Sulfuric a
107. ortable meters accumet AP Series AP 1 and AP 2 pH Meters Durable and reliable portable meters e Measures pH mV and relative mV to 0 01pH and 0 1mV e Dual display shows pH or mV and temperature e Push button pH calibration with auto buffer recognition e Manual or automatic temperature compensation e Store and recall 16 readings with corresponding temperature AP72 meter also features Real time clock stamps stored data and calibration data with date and time e Expanded memory stores up to 50 readings e Selectable pH buffer sets e Standard US NIST and DIN buffers e Selectable automatic endpoint freezes reading when stable e C F selectable Model No AP71 AP72 z Range pH 2 00 to 16 00 N M Flairs e Resolution pH 0 01 C pH Accuracy pH 40 01 6 US 1 68 4 7 10 12 45 NIST 1 68 4 01 6 86 9 18 12 45 or DIN 1 09 2 06 4 65 6 79 9 23 12 75 seeaiiniatian 5 1 68 4 01 7 01 10 01 12 45 Range C 0 0 to 100 0 C 0 0 to 100 0 C 32 0 to 212 0 F Temperature Resolution C 0 1 C 0 1 F Accuracy C 0 5 C 0 5 F General Memory Stores 16 data sets Stores 50 data sets Cat No Description 11590254 AP71 Meter Includes meter AAA batteries and manual 11580254 AP71 Meter Kit Includes meter pH ATC electrode Cat No 11570174 refill solution hard carrying case 60mL sample bottles of storage solution rinse water pH4 and
108. pH6 00 phosphate stabilised with 10ppm mercury II chloride traceable to NIST 10000210 Buffer standard solution pH6 00 phosphate stabilised with 10ppm mercury II chloride traceable to NIST 10685682 Buffer standard solution pH6 00 phosphate stabilised with 10ppm mercury II chloride traceable to NIST 10695682 Buffer BS standard solution pH6 87 phosphate stabilised with 10ppm mercury II chloride traceable to NIST 11482694 Buffer concentrated solution pH6 87 phosphate stabilised with 10ppm mercury Il chloride Fisher OH Buffers Chemical Cat No Description 10457440 Buffer tablets pH7 00 phosphate makes 100mL per tablet 10082521 Buffer standard solution pH7 00 phosphate stabilised with 10ppm mercury Il chloride traceable to NIST 10151570 Buffer standard solution pH7 00 phosphate stabilised with 10ppm mercury II chloride traceable to NIST 10457640 Buffer standard solution pH7 00 phosphate stabilised with 10ppm mercury Il chloride traceable to NIST 10010210 Buffer standard solution pH7 00 phosphate stabilised with 10ppm mercury Il chloride traceable to NIST 10616072 Buffer standard solution pH7 00 phosphate stabilised with 10ppm mercury II chloride traceable to NIST 10535341 Buffer concentrated solution pH7 00 phosphate stabilised with 10ppm mercury II chloride one ampoule makes 500mL 10204440 X Buffer concentrated solution pH7 00 phosphate stabilised with 10ppm mercury Il chloride each
109. pH7 buffers AAA batteries and manual 11570254 AP72 Meter Includes meter AAA batteries and manual 11560254 AP72 Meter Kit Includes meter pH ATC electrode Cat No 11570174 refill solution hard carrying case 60mL sample bottles of storage solution rinse water pH4 and pH7 buffers AAA batteries and manual Replacement Parts and Accessories for AP 1 and AP 2 Meters Cat No Description 11570174 Replacement pH ATC probe plastic body for AP80 and AP70 series 762mm 30 inch cable 11539144 Temperature probe for AP71 and AP72 316 stainless steel 3ft cable 7 am 11539144 Refer to pages 21 to 22 for further information on the range of probes and accessories for accumet meters 17 www eu fishersci com IE UK EN 2014 200 10187 FOCUS ph electrochem master indd 17 08 09 2014 16 15 12 Portable Meters AP 4 and AP84 Dissolved Oxygen Meters Choose AP74 for DO temp only or AP84 for pH DO temp e Durable waterproof design with IP67 rating e No meter warm up required e Adjustable backlit display e Dual display shows mg L ppm or 96 saturation plus temperature e C F selectable e Independent 100 and zero adjustment calibrations e Key in salinity and pressure manually the meter does the rest e Three year warranty AP84 meter also features e pH measurement with auto buffer recognition e Meter displays probe diagnostics view slope offset and mV values AP74 meter also feature
110. probe 19 05mm diameter 25mL to 500mL processing volume 12991161 Solid tip probe 25 4mm diameter 50mL to 1000mL processing volume for 700 watt sonicator 12353745 Sound enclosure with converter clamp 254mm x 254mm x 508mm W x L x H external dimensions for 120 watt sonicator 12893553 Sound enclosure 343mm x 775mm x 330mm W x L x H external dimensions for 500 and 700 watt sonicators 12883563 Stand with 12 7mm x 610mm rod and converter clamp for 500 and 700 watt sonicator www eu fishersci com IE UK EN 2014 200 10187 FOCUS ph electrochem master indd 60 I Pack qty Pack qty I I lI I IL I IL lI lI lI lI lI lI lI lI IL lI lI IL Il 08 09 2014 16 22 06 Vortexer mini e Small compact vortex mixer NEW e Fixed speed 2800rpm e Orbit 4 5mm e Year warranty Cat No _ Description Pack qty 15212985 Vortexer Mini Vortex mixers e Wizard model features unique infrared tube sensing system e Protection IP42 e Speed rpm 0 to 3 000 e Orbit diameter mm 4 5 e Dimensions w x d x h mm 180 x 220 x 70 e Mass kg 2 4 e Electrical supply 230V 50Hz Cat No Description Pack qty 11726744 Vortex mixer Classic 1 11746744 Vortex mixer Wizard 1 SAMPLE STORAGE Bags sample polyethylene sterile e Secure contaminant free bags to ensure dependable analysis results e Made from heavy gauge virgin polyethylene that meets FDA specifications e Can accommodate solid semi solid and liquid
111. r analysis 10589770 Water for analysis 10626852 Water for analysis standard Solutions for Volumetric Analysis O delent Cat No 10040180 10122050 10604922 10743991 10060180 10639672 10264290 10635112 10244340 10335900 10121430 10764181 10010190 10558230 10568230 10000190 10497060 10695872 10538040 10733991 10325710 10419560 10510921 10439560 10522533 10674722 10646262 10080210 10467640 10487830 10488020 10284480 10214440 10020210 10605882 10615882 11478333 10305760 10315710 10528050 10625112 10375800 10294240 10090220 10756101 10459560 10010230 10224010 10244490 10656262 10479750 10112100 10070180 10744181 10358383 10714761 10408220 10111720 10686642 10744951 10676642 Description Acetic acid solution 1M 1N approx 6 ready to use Acetic acid solution 2M 2N approx 1296 ready to use Acetic acid 0 1M 0 1N ampoule solutrate Ammonium iron II sulfate solution 0 025M ready to use Ammonium iron Il sulfate solution 0 1M 0 1 N ready to use Ammonium thiocyanate solution 0 1M 0 1 N ready to use Ammonium thiocyanate concentrated solution 0 1M 0 1N ampoule Barium chloride solution 1M 2N ready to use Cerium IV sulfate solution 0 1M 0 1N ready to use Cerium IV sulfate concentrated solution 0 05M 0 05N ampoule
112. rature in C The AP72 meter also reads temperature in F Both meters have excellent 0 01pH accuracy The ergonomic design of the AP71 and AP72 meter fits comfortably in your hand Use the detachable electrode bracket to position the electrode for one handed operation Advanced setup mode lets you customise meter parameters and check electrode slope and offset to ensure electrode accuracy The AP71 and AP72 pH meters are ideal for basic pH and mV readings in both harsh environmental conditions and general laboratory applications The AP74 and AP84 meters read dissolved oxygen in mg L ppm or 96 saturation They use barometric pressure and salinity offset to ensure high accuracy with independent 10096 zero and offset adjustment capabilities The adjustable backlit display is ideal for taking readings in low light Probe includes 10ft standard cable length These meters also feature a galvanic probe which allows the user to take dissolved oxygen measurements immediately without the warm up time of other probe designs Advanced features also include display of probe diagnostics The waterproof dustproof housing floats and is very easy to clean The AP84 meter also takes pH readings with 0 01pH accuracy The AP75 and AP85 meters measure both conductivity and TDS plus temperature with a housing that s perfect for rough conditions These waterproof dustproof meters float and are very easy to clean Advanced setup mode lets you customise meter parameters
113. ready to use Potassium hydroxide solution 0 5M 0 N alcoholic ready to use Potassium hydroxide solution 0 5M 0 5N alcoholic ready to use Quantity 100gr 1L 2 5L 10L 25L Quantity 2 5L 2 5L 6 ampoules 2 5L E JE 6 ampoules 1L E 6 ampoules 6 ampoules 2 5L E IL 2 5L 10L 6 ampoules 2 5L 2 5L TL UE 2 5L BL 10L E 2 5L IE 2 5L E 2 5L 5L 10L 1L 2 5L i 25L 5L 6 ampoules 6 ampoules 6 ampoules ub i 2 5L iL i 6 ampoules 1L TE 2 2 5L 100mL 6 ampoules TL 100mL 100mL 100mL es 2 5L 6 ampoules 1L E TL 2L Fisher Chemical For up to date GHS information on Fisher Chemical products listed please refer to the safety data sheet available from www eu fishersci com www eu fishersci com IE UK EN 2014 200 10187 FOCUS ph electrochem master indd 45 45 08 09 2014 16 20 52 Fisher Chemical 46 10366112 10080190 10020200 10598420 10346140 10191760 10705721 10326190 10294670 10214680 10124713 10449370 10060220 10111910 10640423 10746101 10725911 10366330 11402914 10316430 10776291 10496870 10538050 10508240 10121670 10734951 10070190 10141860 10558050 10637032 10224630 10316190 10040200 10436602 10438410 10151810 10765141 10528240 10745141 10755141 10666452 10070200 1003020
114. s Real time clock stamps stored data and calibration data with date and time e Memory function stores and recalls up to 50 readings CUCINUUSITUEES mene P 4 e lt m a siti name Gene Sts t 9 N Model No AP74 AP84 Concentration mg L or ppm 0 00 to 20 00 HON Saturation 0 00 to 200 0 Do Resolution DO Concentration mg L or ppm 0 01 Saturation 0 1 Accuracy DO 1 5 full scale Calibration 2 Point 0 100 1 point mg L Range pH 2 00 to 16 00 Resolution pH 0 01 pH Accuracy pH 0 1 pH calibration 5 1 68 4 01 7 00 10 00 12 45 Range C 0 0 to 50 0 Temperature Resolution C 0 1 Accuracy C 0 3 Memory Stores 50 data sets General Dimensions mm 190 x 95 x 57 Mass g 600 Cat No Description 11530254 AP74 Meter Includes meter dissolved oxygen temp probe Cat No 12784506 electrolyte _two membrane cap assemblies polishing disk AAA batteries and manual 11520254 AP74 Kit Includes meter dissolved oxygen temp probe Cat No 12784506 electrolyte two membrane cap assemblies Cat No 10030073 and hard carrying case polishing disk AAA batteries and manual 11530254 AP84 Meter Includes meter dissolved oxygen temp probe Cat No 12784506 electrolyte _two membrane cap assemblies Cat No 10030073 polishing disk AAA batteries and manual 11520254 AP84Kit Includes meter dissolved oxygen temp probe C
115. s Thermometer calendar timer Iraceable M e Temperature range 23 to 122 F and 5 to 50 C e Shows time date day of the week and temperature e Huge 54mm digits provide superior visibility e Monitors room temperature in C or F e Accuracy 0 0196 Cat No Description Pack qty 11795833 Thermometer calendar timer Traceable a Thermometer wide range Iraceable e Designed for rough handling e Fast response microprocessor updates the display twice a second e Water resistant case e Range is 328 to 2498 F and 200 to 1370 C e Resolution of 0 1 and 1 e Accuracy 1 C between 50 C to 740 C e Dimension mm 82 5 x 178 x 38 Cat No Description Pack qty 11749765 Thermometer wide range Traceable a Thermometer digital infrared with pocket clip Traceable M e Point and shoot at any surface and take a reading in less than one second e Non invasive no touch measurements ideal for food preparation life sciences pharmaceuti cals petroleum clean rooms electronics and field use e Range 33 C to 220 C and 27 F to 428 F e Resolution 0 1 C e Accuracy 1 C e Dimensions mm 83 x 19 Cat No Description Pack qty 11709785 Thermometer infrared supplied with battery key 1 chain lanyard and Traceable certificate Thermometer clock numidity meter digital Iraceable M e With an ISO 17025 A2LA Traceable Certificate to NIST National Institute of Standards Technology e Cl
116. solution 2M 2N ready to use Hydrochloric acid solution 5M BN ready to use Hydrochloric acid solution 5M 5N ready to use Hydrochloric acid solution 5M 5N ready to use Hydrochloric acid concentrated solution 0 1M 0 1N ampoule Hydrochloric acid concentrated solution 0 5M 0 5N ampoule Hydrochloric acid concentrated solution 1M 1N ampoule lodine solution 0 05M 0 1N ready to use lodine solution 0 05M 0 1N ready to use lodine solution 0 5M 1N ready to use Nitric acid 6096 concentrated solution 1N ampoule Nitric acid solution 0 1M 0 1N ready to use Nitric acid solution 1M 1N ready to use Nitric acid solution 1M 1N ready to use Nitric acid solution 2M 2N ready to use 1 10 phenanthroline ferrous pure solution 0 025M ready to use Potassium bromate bromide concentrated solution 0 0167M ampoule Potassium bromate bromide solution 0 0167M 0 1N ready to use Potassium chloride 4M 4N pure electrode filling solution Potassium chloride 4M pure electrode filling solution with silver chloride Potassium chloride pure saturated electrode filling solution Potassium chloride solution 3M ready to use Potassium dichromate solution 0 0167M 0 1N ready to use Potassium dichromate concentrated solution 0 0167M 0 1N ampoule Potassium hydroxide solution 0 1M 0 1N alcoholic ready to use Potassium hydroxide solution 0 1M 0 1N alcoholic
117. st be present in the sample for the best measurement results e The standard Ag AgCl electrodes micro Ag AgCl electrodes and economy electrodes contain a single junction silver silver chloride reference that will become clogged in solutions that contain silver complexing or binding agents such as TRIS buffer proteins and sulfides Frequent cleaning may be required when measuring these solutions which will shorten the electrode life Proteins cause the additional problem of coating the pH sensing bulb so extra care should be taken to keep the electrode clean while measuring samples that contain proteins oH Measurement Procedure 1 Verify that the pH electrode has been prepared and calibrated correctly If the electrode is refillable make sure that the fill hole is uncovered and the filling solution level is at least one inch above the sample solution level 2 Rinse the electrode with distilled water and blot it dry with a lint free tissue 3 Place the electrode into the sample Use a magnetic stir plate and clean stir bar or the accumet benchtop stirring probe to stir the sample at a moderate uniform rate 4 When the measurement is stable record the pH value and temperature of the sample Measurement Hecommendations e Check the electrode slope daily by performing at least a two buffer calibration The slope should be 92 to 102 e Unless otherwise specified only use the recommended filling solution in refillable pH electrodes e f
118. the measured temperature Refer to the meter instruction manual for a detailed procedure The table on page 34 provides pH values for buffers at various temperatures The meter should display a 92 to 102 slope or 54 43 to 60 34mV per pH unit depending on the pH meter Refer to the meter instruction manual for details on how the meter displays the calibration information p EH N 33 www eu fishersci com IE UK EN 2014 200 10187 FOCUS ph electrochem master indd 33 08 09 2014 16 16 37 Technical Resources One buffer 1 Verify that the pH electrode was prepared correctly and connect the electrode to the meter 2 Select a pH buffer that is near the expected sample pH The pH buffer should be at same temperature as the samples If the buffer and samples are at varying temperatures temperature compensation is recommended 3 Rinse the electrode with distilled water and blot it dry with a lint free tissue 4 Place the electrode into the buffer When the reading is stable set the meter to the pH value of the buffer at the measured temperature and set the calibration slope to 100 or 59 16mV per pH unit depending on the pH meter requirements Refer to the meter instruction manual for a detailed procedure Please note that a single point calibration is not as accurate as a multi point one and should only be done when absolutely necessary as it relies on a near perfect electrode to give good readings Where possible always use a mi
119. three atmospheres with O ring seal e Times to 24hr e Seconds resolution for first 30min e Accuracy 0 01 e Dimensions mm 53 x 59 x 12 5 Cat No Description Pack qty 11755833 Waterproof Shockproof Stopwatch Traceable i Timer Clip It M Traceable e Counts down alarms and has stopwatch mode e Clips to your lab coat belt notebook clipboard or anywhere e Six digit LCD display shows the time remaining in hours minutes and seconds e Dimensions mm 51 x 38 x 19 e iming range 100 hours to 1 second Resolution 1 second Cat No Description Pack qty 11765873 Timer Clip It V Traceable 1 Timer countdown clock Traceable e Double display e Fach channel has a distinct alarm e Automatically counts up from zero e ABS impact resistant case e Resolution 1 second e Accuracy 0 01 e Dimensions mm 76 x 63 5 x 13 Cat No Description Pack qty 11507493 _Timer countdown clock Traceable a 66 www eu fishersci com IE UK_EN_2014_200_10187_FOCUS_ph_electrochem_master indd 66 08 09 2014 16 22 36 Timer count down up Iraceable M e Counts down up to from 24hr with alarm e Two timing channels e Memory e Clock Timer Description Pack qty 12695296 Countdown up timer Traceable 1 Timer extra extra large digit Iraceable M e Double display e Extreme digit size for easy viewing e imer countdowns and alarms on single channel for ease of use e Counts up during alar
120. tion TechSupport You will need pH meter Electrode Wash bottles pH buffers t pages 10 to 20 pages 27 pages 31 pages 29 to 30 pages 45 The following is a general procedure for preparing most pH electrodes However it is important that you also refer to the user guide or instruction manual for your particular electrode for any specific preparation requirements 1 Remove the protective shipping cap sleeve or bottle from the electrode pH sensing bulb and save the cap sleeve or bottle for storage If the electrode has a storage bottle covering the pH sensing bulb unscrew the storage bottle cap before removing the electrode from the storage bottle 2 Clean any salt deposits from the electrode exterior by rinsing it with distilled water 3 If the electrode is refillable uncover the fill hole and add the appropriate filling solution to the electrode To maintain an adequate flow rate the level of filling solution must always be above the reference junction and at least one inch above the sample level The fill hole should be open whenever the electrode is in use 4 Gently shake the electrode downward similar to a clinical thermometer to remove any air bubbles that may be trapped inside the electrode 5 Soak the electrode in a standard pH electrode storage solution for at least 30 minutes 6 Connect the electrode to the meter 7 Select at least two standard pH buffers that bracket the expected sample pH 32 Www eu fis
121. torage up to one week soak the electrode in pH electrode storage solution To minimise the chance of breakage the micro pH electrodes should be attached to an electrode stand and suspended in a beaker that contains storage solution The electrode should not touch the sides or bottom of the beaker ong Term Electrode Storage more than one week If the electrode is refillable fill the reference chamber up to the fill hole with the appropriate electrode filling solution and securely cover the fill hole with parafilm Cover the pH sensing bulb and reference junction with a protective cap sleeve or storage bottle containing storage solution Before returning the electrode to use prepare it as a new electrode pH Electrode Cleaning Procedures TechSupport You Will need Transfer pipette Electrode Wash bottles Beaker pages 56 pages 27 pages 31 pages 47 pages 45 One of the most common reasons for a pH electrode not working properly is because it is dirty clogged or coated with sample Cleaning a dirty clogged or coated electrode restores proper electrode performance and prolongs its useful life The following instructions provide a starting point for developing an effective cleaning procedure 1 Choose a suitable cleaning solution 2 Shake the cleaning solution Pour enough of the cleaning solution into the beaker to cover the electrode junction 3 Soak the electrode for five to ten minutes in the cleaning solution while moderatel
122. trode and sensing electrode potentials in millivolts The millivolts are then converted to pH units and shown on the meter display The sample or standard solution is the final component of the system Electrode behaviour is described by the Nernst equation E E 2 3 RT nF log aH Where E is the measured potential from the sensing electrode E is related to the potential of the reference electrode 2 3 RT nF is the Nernst factor and log aH is the pH The Nernst factor 2 3 RT nF includes the Gas Law constant R Faraday s constant F the temperature in degrees Kelvin T and the charge of the ion n For pH where n 1 the Nernst factor is 2 3 RT F Since R and F are constants the factor and therefore electrode behaviour is dependent on temperature The electrode slope is a measure of the electrode response to the ion being detected and is equivalent to the Nernst factor When the temperature is 25 C the theoretical Nernst slope is 59 16mV pH unit Fisherbrand accumet pH meters display the slope as a percentage of the theoretical value For example a 98 596 slope is equivalent to a slope of 58 27mV pH unit for a two point calibration The pH meter detects the pH sensing bulb signal reference signal and temperature signal and uses these values to calculate the pH using the Nernst equation Fisherbrand accumet pH meters contain pH versus temperature values for commonly used buffers This allows the meter to recognise a particular pH bu
123. uced under strict ISO 9002 EN 29002 standards CE marked No 98 06 0119 Class II A Cat No Description Dimensions mm Pack qty Sterile in tube sterilised by ethylene oxide 11502483 Wood with cotton tip 150x25 500 11512483 Polystyrene with cotton tip 1503 2 5 500 11522483 Aluminium with cotton tip 150x0 9 500 11532483 onappable polystyrene with PETE tip 150 x 2 5 500 Sterile in peel pack sterilised by ethylene oxide 11552483 Snappable polystyrene with cotton tip 10x25 1 000 11542483 Wood with cotton tip 150x25 1 000 Transport swabs sterile in peel pack sterilised by irradiation expires 30 months after sterilisation date 11562483 Amies Snappable polystyrene applicator 150 x 2 5 100 With viscose tip 12977960 Amies with charcoal Snappable E 100 polystyrene applicator with viscose tip Non sterile 11572483 Polypropylene with cotton tip 150x25 4 000 11582483 Snappable polystyrene PETE tip 150x25 4 000 11592483 Aluminium with cotton tip 150x 0 9 1 000 12977970 Wood with cotton r 10 000 www eu fishersci com IE UK EN 2014 200 10187 FOCUS ph electrochem master indd 51 Fisherbrand Supplement ol 08 09 2014 16 21 11 Fisherbrand Supplement SAMPLE IDENTIFICATION OCounter Pen M digital meter e Beep at each keystroke for safe and effective counting e LCD display with counting up to 99 999 units e Total count function and easily accessible reset button Cat N
124. vesesetevsnsevevevsesvenetsssesetees 62 e Thermometers and Timers sss 63 67 www eu fishersci com 08 09 2014 16 14 04 Electrochemistry FOCUS ON WATER ANALYSIS WORKFLOW Depend on Fisherbrand Fisher Chemical and Fisher Bioreagents to provide products for every step of your water analysis workflow Microbiology Legionella E coli Enterococcus Pseudomonas Staphylococcus Streptococcus Salmonella Incubators Safety cabinets Microscopes Colony counters Petri dishes Media Swabs Elementary Analysis Main Na K Ca Mg Secondary Pb Hg Cr As Sc ICE 3300 for one element ICAP for multi elements Mono elementary standards i Multi elementary standards t Vortexers Sample Preparation Balances Centrifuges Centrifuge tubes Chemicals Dialysis tubing Overhead stirrers Pipettors and pipettor tips Sonic dismembrators Syringe filters Chromatography Pesticides traces LCMS Columns HPLC Solvents GC Chemicals Flasks Syringes es e e e s 0 s 20222222 22 252 22023202059292292292229292229259292592929292929292929222929292929225292929252525 IE UK EN 2014 200 10187 FOCUS ph electrochem master indd 6 e Benchtop pH meters e Benchtop conductivity meters e Benchtop dissolved oxygen meters e Electrodes e pH buffers e Conductivity standards e pH indicator sticks reels e Thermometers Li e Timers www eu fishersci com 08 09 2014 16 14 07 F
125. y stirring the solution Electrodes with wick junctions may require more cleaning time 4 Remove the electrode from the cleaning solution and rinse it thoroughly with distilled water 5 If cleaning a refillable electrode remove the filling solution from the electrode using a plastic pasteur pipette and add fresh filling solution to the electrode Repeat this step two or three times for optimal electrode performance 6 If cleaning a flushable electrode flush a few drops of filling solution through the electrode junction by pressing down on the electrode cap Ensure that the junction flushes and resets properly Refill the electrode with filling solution 7 Soak the electrode in standard pH electrode storage solution for at least 30 minutes 8 Rinse the electrode thoroughly with distilled water and measure samples as usual If the electrode response is slow or the electrode does not calibrate correctly repeat the cleaning procedure Viscous samples and samples that contain solid materials often require additional cleaning and additional filling solution changes 3 www eu fishersci com 08 09 2014 16 18 26 Technical Resources General Rules and Tips TechSupport e When using refillable electrodes open the fill hole during calibration and measurement but remember to close it afterwards when finished e he level of electrolyte in the outer cavity of refillable electrodes should be kept above the level of the solution being me

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