Unico SS-1.22
Contents
1. Step 4 Step 5 UNICO Application Software optional Click Print button print the graph and labels of the slope y intercept and Correlation Coefficient as seen on the screen When ready to measure samples of unknown concentration press arrow return button to return to the main Standard Curve screen Figure 31 To measure the concentration of unknowns place the samples in the Sample Compartment and click on Test button Figure 31 located at the bottom left portion of the screen To save the data with the Standard Curve fit selected click on the Wl Save Icon name the file and click Save dcm mw E pee Sante sen e vence DIE oa re a HA Figure 31 Sample Test Results of Standard Curve UNICO Application Software Ly Ve ins nz pono joo Dislalia alale z Tene eE m m l A c II Figure 32 Standard Curve Graph Display meus Ges Figure 33 Buttons for Standard Curve Test Kinetics The Kinetics application has the following functions Setup kinetics Test Parameters Obtain kinetics data for a sample at a single wavelength Load and save data files for further studies Get the reaction rate within a given time interval Main Screen of Kinetics A the Main Screen Figure 13 click Application Drop down Menu click Kinetics Figure 15 to enter the Main Screen of Kinetics Test Fi2. New reset Text Parameter and begin new test Load open any saved file tst UNICO Application Software Save as save the setup or data displayed Print print all information shown in the Data Screen and any data collected Exit quit the VIS1200 Application Drop down Menu can select three Analytical Methods of the 1200 1201 Spectrophotometer A Trans Cone Kinetics and Standard Curve A Trans Conc measure Absorbance Transmittance Concentration Standard or Concentration Factor Kinetics measure a sample s Absorbance change over a selected period of time store the test results in data table display the results graphically and calculate the reaction rate within a given time interval Function Menu is part of the Kinetics Analytical Method Standard Curve create a calibration curve choice of 4 curve fits with up to 8 standard solutions to determine Concentrations of unknown samples Text Field Three Text Fields are just below File Drop down Menu and under the label Current WL nm Abs and Trans Figure 16 ere WLC Ate setta zZ p 000 Im Figure 16 Text Field Current WL nm Text Field display the last selected wavelength of the spectrophotometer Abs Text Field display the current data in Absorbance Trans Text Field display the current data in Transmittance Icon Button Seven Icon Buttons are below the Text Field Figure 17 Ble lS Tew Figure 17 Ico
3. Next button Step 5 Click the Finish button and the test data will be imported into your Excel spreadsheet Here further calculations can be performed from the raw data collected Step 6 Save the imported file under a DIFFERENT FILE Name if you still want to keep and open the original UNICO data file in VIS1200 Otherwise the original UNICO data file tst file will be modified by the Excel format during importing and the modified file cannot be opened from VIS1200 TE Senes deem e Pe dun A sees MZ nemen ns al i Imppp IS msec seit n m E md i r Figure 4 Excel Text Import Wizard 21 UNICO Application Software Figure 42 Excel Text Import Wizard Il 28
4. Standard or Concentration Factor at a single wavelength within the range of 325 1000 nm Standard Curve create a calibration curve choice of 4 curve fits with up to 8 standard solutions at a single wavelength to determine concentrations of unknown samples Kinetics Absorbance vs Time Kinetics measure a sample s absorbance change over a selected period of time store the test results in data table display the results graphically and calculate the reaction rate within a given time interval Minimum Computer Requirements To properly install and operate the enclosed software it is required to have the following minimum computer configuration PC with 16MB RAM Pentium or faster processor 10 MB of free space on memory VGA Color Monitor PS 2 mouse and keyboard NOTE The UNICO Application Software provided will NOT function with a Macintosh Apple or Linux computer UNICO Application Software Software Installation Loading Software to Computer To install the software please close any open programs and disconnect from the Internet if online then follow the instructions below Step 1 Insert UNICO Application Software CD into the CD drive of your PC If the software can show the Automatic Setup Screen as Figure 1 go to Step 4 If it can not go to Step 2 Step 2 Double click My Computer icon of your PC and locate the CD Drive as Figure 2 indicates Step 3 Right click the CD Drive Label and click O
5. 2C port on the back of your PC Connect the female 9 pin small connector one end of the Connection Cable to the male 9 pin of UNICO Application Software your PC and secure with the built in screws If your PC does not have a male 9 pin connection use the male 25 pin connector ONLY USE The 25 pin connector if your PC does not have a male 9 pin connector common for older upgraded computers Step 3 1200 1201 Spectrophotometer has a male 9 pin RS 232C port on the back panel located to the left side of the 1200 1201 Connect the other end of the connection cable to your 1200 1201 and secure tightly Step 4 Tum on your PC if not already on and your 1200 1201 if not already on and let it warm up for fifteen minutes Step5 Click the Start button on your PC scroll to Programs UNICO Application Software and locate the VIS1200 Figure 10 and click it Step 6 The Start up Screen of VIS1200 will appear as Figure 1 1 shown Step 7 Push the ENT key on the 1200 1201 panel select the proper port and then click OK on your Computer Screen The VIS1200 will initialize and go to the main screen Figure 12 CAUTION Do NOT use any button on the 1200 1201 panel while the VIS1200 is running and connected with 1200 1201 Otherwise the data collected may not be correct EI Arcore IS Ceaive IS HP Lawnt 2200 ME BSN Eat IS Queldere ES QuickBooks Pro S siete Mint rere Cien WB eere Enlai i YemMerenum ssa Piet Gi
6. Ou Esp PeesiPineCD Plu 20 Eri LS Window esonor IS Morone EA Nen Olim Desiment aeg Demn Office Document pd N E 4 Lon OI anie Shu Bown Figure 10 IS Powertques Pariteriiagie 70 IS Dire Startap lerre IS Post Beata 7 0 IS Wie IS mono gt LS OLYMPUS CAMEDIA Matter Quek Tine IS meren Phaser 350 SH npe Hoton Artie Open the Software UNICO Application Software TO UNICO Application Saltare URLO Figure 11 Start up Screen Main Screen Display After your 1200 1201 is connected to your PC and VIS1200 is running your PC will show the Main Screen Display like Figure 12 Type your User Name in the Text Field at the right of the User Name U label or leave it blank then click the OK button to login as Figure 13 shown Figure 12 Main Screen Display UNICO Application Software ST i e EE LLL Figure 13 Main Screen Display After User Login Functions of Drop down Menu Text Field and Icon Button Drop down Menu Two Drop down Menus File Figure 14 and Application Figure 15 are illustrated below User Cte Ciel CiS Clee Cube supp Figure 14 File Drop down Menu A hens Cone kinetics Standard Curve Function Figure 15 Application Drop down Menu File Drop down Menu has six functions New User New Load Save as Print and Exit All of them have hot keys except the first one New User change user name
7. Trans Conc At the Main Screen Figure 13 click Application Drop down Menu and click A Trans Conc Figure 15 to enter the Main Screen of A Trans Conc Figure 20 13 UNICO Application Software dire die eco ille alejes Wovetnan E Titti e Tae Team 0000 pm ES Oo iron Teer Vieni 00 Figure 20 Main Screen of A Trans Conc The A Trans Conc Analytical Method has five operation functions Test Wavelength Text Field Test Parameter Test Field TEST Button Data Table and Test Date Test Wavelength Text Field select the desired wavelength by moving the dial on the spectrophotometer and clicking 2x1 button on the PC screen click OK button Figure 21 at the right of Z2 button to set the wavelength Figure 22 The wavelength will be shown both in the Text Field below Current WL nm label and Text Field at the right of the Test Wavelength label Test Parameter Text Field If standard solution is available type the concentration values of the standard into the Text Field at the right of Conc Std label insert the standard into Sample Compartment and click the OK button Spectrophotometer will take measurement of the standard and display factor value The unknown sample concentrations can then be measured If factor value is available enter the factor value into the Text Field at the right of Factor label for the unknown sample concentrations calculation Figure 21 TEST Button record th
8. and maximum Absorbance range for the graph of the data Step 9 Place your sample in the Sample Compartment and click the Start Button once all the Test Parameters have been set and you are ready to start your measurement Figure 36 Notes Data Table once measurements have been made clicking this button will allow you to view the data collected Figure 37 Only Cell 2 of the Data Table is currently used to store the data collected others are for future applications Stop Button stops the data collection at any given moment p eesrantine Fr se 1 ES in ES oo MI imum F om om e E e n ai e Tecos ecu enc oc Tos d Figure 36 Sample Test Results of Kinetics UNICO Application Software 3 UNICO kinetics Test Data Gl x Data 2 ee a E o 147 460 13170 3 iz so 1007 2 iz 510 12 al 1x9 49 130 dl ixi 40 131 5 134 4m 1324 2 B 133 4 amp 0 1338 8 a i45 358 1456 a isa 290 156 S id 32 140 _ Tol 150 am 15 di 1207 8200 1287 32 29 530 129 E fl az 53 129 z dal 122 sa 1272 dl 125 580 12 a8 1199 63 1199 fl ti em ts JB ti 70 i054 Jj timo 730 11 2 ins 78 119 E Zi ino 70 ino 2 106 84 10065 x gt Figure 37 Kinetics Data Table Math Function for Kinetics VIS1200 provides additional features for you to calculate
9. e readout of a given sample Figure 23 Data Table record ID Name Abs Trans Conc and Factor related with the test Figure 23 UNICO Application Software Test Date Test Date will be displayed automatically To alter it simply click it at the left corner of the screen and enter the desired date Figure 24 Test Parameter Cows D Wavelength a TestWevelength o oK Factor Figure 21 Test Wavelength and Test Parameter Absorbance Transmittance Mode The following are the basic operations Figure 25 Step 1 Insert reference cuvette or nothing into the Sample Compartment and close the lid Step 2 Select the desired wavelength by moving the WAVELENGTH dial on the Step3 Click on 0A 100 T Icon Button 1200 1201 Click button on the PC screen to match the desired wavelength click OK button at the right of wavelength button to set the Wl button the blue cuvette icon to blank the reference DO NOT PUSH ANY BUTTONS ON THE 1200 1201 THIS WILL CAUSE FOR YOU TO RE BOOT THE SOFTWARE Step 4 Remove the reference if it is there and place your sample cuvette into the Sample Compartment close the lid and click on TEST Button The test results will be displayed in a spreadsheet format in the Data Table Step5 You may type in sample name or change the sample name under the Name label inside the Data Table 15 UNICO Application Software Fig
10. ew existing standard curves Calculate the Concentrations of unknown samples Main Screen of Standard Curve At the Main Screen Figure 13 click Application Drop down Menu and click Standard Curve Figure 15 to enter the Main Screen of Standard Curve Figure 29 Td Zan Tettone 2 oe me Tn ceca OT Figure 29 Standard Curve Setup The following are the basic operations Step 1 Insert reference cuvette or nothing into the Sample Compartment and close the lid Step 2 Select the desired wavelength by moving the WAVELENGTH dial on the 1200 1200 Clic utton on the PC screen to match the desired 19 Step 3 Step 4 Step 5 Step 6 Step 7 UNICO Application Software wavelength click OK button at the right of ES button to set the wavelength Click on 0A 100 T Icon Button blank the reference Under Standard Sample Setting click the Arrow Button and set the number of standards from 2 to 8 to be used Figure 30 Place the standards in the Sample Compartment in order of lowest concentration to highest concentration Type the concentration of the standards e g 0 14 here into the Text Field starting below Conc label and at the right of ID 01 as Figure 31 shown Press Enter key on your computer keyboard or click with the mouse to move to the next cell Repeat the same operation until all concentrations of the standards have been entered Measure the Absorbance o
11. f each standard For each measurement insert the standard into Sample Compartment and click Test button The Absorbance and Transmittance values will be recorded into the Data Table then double click in the appropriate Absorbance cell Abs in the left table next to the standard Conc to get the Absorbance for curve drawing Continue until all of the standards have been measured Figure 31 Alternatively if you do not want the Absorbance of the standards to be recorded into the Data Table you may select the appropriate Absorbance cell Abs in the left table next to the standard Cone and double click the mouse to get the Absorbance results Press the Define Curve Button Figure 31 to graph the Standard Curve e g WaterS 1 to WaterS 6 are the standards Figure 32 and you may save it for future use Wl button the blue cuvette icon to Standard Sample Setting Samples Figure 30 Standard Sample Setting Your standards have now completed setup To use the graph and measure the unknown sample concentrations please be guided by the steps below Step 1 Select the desired Standard Curve by clicking on one of the four buttons Figure 33 Shown in Figure 32 is Linear Squares Least Squares Method Items in the equation next to the Linear Squares title on the graph are Abs the slope and y intercept as well as Correlation Coefficient Abs slope C y intercept Corr Coef Correlation Coefficient Step 2 Step 3
12. gure 34 The following are the basic operations Step 1 Insert reference cuvette or nothing into the Sample Compartment and close the lid Step 2 Select the desired wavelength by moving the WAVELENGTH dial on the 1200 1201 Click ZLI button on the PC screen to match the desired wavelength click OK button at the right of Z1 button to set the wavelength 22 UNICO Application Software Step 3 Click on 0A 100 T Icon Button IH button the blue cuvette icon to blank the reference Figure 34 Kinetics Test Main Screen Figure 35 Test Parameters Setting Step4 Set the Test Parameters Figure 35 Step 4 to Step 8 Set the Total Run Time by typing into the Text Field on the right of the Total Run Time label This is the total time for the reaction to occur Step 5 Set Sampling Interval time similar as Step 4 This is the time interval for which measurements will be recorded i e every 10 s or every 3 s etc Step6 Setan Initial Time Delay similar as Step 4 The purpose of this stop is to delay the beginning of data collection i e Sample must be injected and reaction will not begin for 20 s 23 UNICO Application Software Step7 Set a Conc Factor multiplication factor dilution factor similar as Step 4 This allows for a factor to be used when calculating the Concentration of the solution Step 8 Set the High and Low Limits of the graph similar as Step 4 This is the selection of the minimum
13. is Hep El louem 8 2 xag oy Ton O G om Fate ume te Pere Ve Add 8 my Computo m obo ari E Nane Tye Tods Fire Space BI Fg loch Fp Dik Sic Lesa TT ei Lai ime ia ly Computer Sn tan iwa Uma Sri pea de ima tam Sum uam PE porte en Local Disk 38x8 35408 Sn pera ims a Bosse SU 2e Cire voas iie Sor ElDdUpleelao Seni Sent Sona D Baram Suoni Bis Cin CIO Store Fre once Figure 2 Locate CD Drive Screen UNICO Application Software E En sei BY Computer Los EY E Sue Sol ety 08 me pp n e bytes Figure 3 Open the CD E A p 1200 e 3 p m E ans TERA via por sue Sure see 1388 Saunas Figure 4 Click setup exe UNICO Application Software UNICO Application Software For VIS1200 Setup EUW ca Stn For WTS Ener HE dengn aa ila Eu aia o pcs nn sy BEI UR pw a Figure 5 Setup Screen UNICO Application Software For VIS1200 Setup entri ET ihnen asset hoe ey Seance Figure 6 File Setup Directory Selection PowerQuest BoctMagic 7 0 PowerQuest PartitionMiagi 7 0 Figure 7 File Setup Directory Selection Il Figure 8 Install Icon UNICO Application Software For VIS1200 Setup p Figure 9 Installation Success Connecting Computer to Spectrophotometer Step 1 Remove the RS232 Connection Cable double end out of the bag Step 2 Locate the RS 23
14. n Button The four Icon Buttons counting from the left end of the Figure 17 have the same functions as New Load Save as Print of the File Drop down Menu 0A 100 T Icon Button set 0 Absorbance and 100 T Insert reference into Sample Compartment and click M button blue colored move cursor on to the blue cuvette icon the fifth icon from left end of Figure 17 and right touch the mouse it will show 0A 100 T sign Figure 18 UNICO Application Software 08 100 Figure 18 0A 100 T Icon Button for BLANKING 0 T Icon Button set 0 T Remove cuvette from Sample Compartment before clicking Mil button black colored move cursor on to the black cuvette icon the sixth icon from left end of Figure 17 and right touch the mouse it will show 0 T sign Figure 19 ox Figure 19 O amp T Icon Button Reset Button unlock the screen If the screen frozen due to improper operations BLANK may show in the Text Fields below Abs and Trans labels Figure 16 click l button to reset them and unlock the screen Basic Operation Three Analytical Methods Absorbance Transmittance Concentration Standard Curve and Absorbance vs Time Kinetics are illustrated below Absorbance Transmittance Concentration The Absorbance Transmittance Concentration A Trans Conc method has the following three modes of operation Absorbance Transmittance Concentration Standard Concentration Factor Main Screen of A
15. pen as shown in Figure 3 Step 4 Click setup exe as Figure 4 shown or click SETUP shown in Figure 1 Step 5 The Setup Screen Figure 5 will then display a Welcome message reminding you to close any open programs If all programs are closed then click OK and go to Step 6 If other programs are open and operating click Exit Setup close the open programs and return to Step 2 Step6 Choose the Directory and Program Group where you want the software to be installed as shown in Figure 6 and Figure 7 The File Setup Directory 1 Figure 6 will then appear If the File Path selection is OK then go to Step 7 NOTE if you wish to change the location of the installation then click Change Directory and specify the desired directory If you are unsure and need to examine your computer files click on Exit Setup and go to Windows Explorer to make sure no duplication or improper storage of the files will occur Setp 7 Click the Install Icon Figure 8 to start VIS1200 installation click Continue to finish the installation Step 8 Wait for the message stating the software was installed successfully as shown in Figure 9 Click OK re start your PC Congratulations You have now installed your UNICO Application Software VIS1200 in your PC for your Model 1200 1201 Spectrophotometer UNICO Application Software ETUP United Products amp Instrument 182 E Ridge Road Dayton NJ Figure 1 Automatic Setup Screen Se Eh Yew Go Fo
16. s with known multiplication factor to calculate the Concentration Repeat Step 1 to 3 in Absorbance Transmittance Mode section Step 4B Type in the desired Factor in the Text Field at the right of the Factor label Step 5B For each sample be sure to place the sample in Sample Compartment and close the lid Click the TEST Button to record results The Absorbance Transmittance and Concentration of the samples will be displayed in the Data Table Figure 28 olaga seie ee price eel how ol A RE Figure 28 Sample Test Results of Concentration Factor Mode 18 UNICO Application Software Standard Curve The Standard Curve Calibration Curve method allows the operator to Measure up to 8 standards Calculate standard curves with 4 curve fits including 1 Linear Thru Zero set the y intercept equal to zero therefore the curve is forced through zero Calculate and display the slope and Correlation Coefficient 2 Linear Squares Linear regression model Calculate and display the slope y intercept and Correlation Coefficient for the given standards 3 2 Order second derivative of the Linear Squares model Calculate and display the coefficients This method is used for non linear standard curves or curves with a Correlation Coefficient of less than 0 9 4 Segmented straight line Use the standards as nodes to connect each point No data is displayed or calculated Select and vi
17. the reaction rate within a given time interval After your Kinetic test keep the test curve screen open Click Application Drop down Menu and click Function as Figure 38 shown Click K type the start end time and factor into the appropriate Text Fields in the pop up window as Figure 39 indicated Press confirm button to get the reaction rate graph Figure 40 If you want to get another reaction rate for a different time interval simply go to Function click Replace it will return to the original test curve screen repeat the above mentioned procedure you will get the new reaction rate graph UNICO Application Software Figure 38 Math Functions for Kinetics E Unico Function Figure 39 Math Function Setting Ab VS Time Kansaso bet944100 A H80 00000 km Figure 40 Sample Test Results of Math Function of Kinetics 26 UNICO Application Software Data import to Microsoft Excel By referring to the following steps you can transfer any of the UNICO Application Software VIS1200 test data to a Microsoft Excel program Step 1 In Microsoft Excel click on File and click Open Step2 Select any saved file you wish to import Step 3 After the Excel Text Import Wizard appears select Delimited select the row from which you want the import to start and click on the Next button as shown in Figure 41 Step 4 Uncheck the Tab delimiter and select Comma delimiter as shown in Figure 42 then click the
18. unico 1200 SERIES SPECTROPHOTOMETER SOFTWARE USER S MANUAL Version SS 1 22 UNICO Application Software Table of Contents Introduction 3 Minimum Computer Requirements 3 Software Installation 4 Loading to Computer 4 Connecting Computer to Spectrophotometer 8 Main Screen Display 10 Functions of Drop down Menu Text Field and Icon Button 11 Basic Operation 13 Absorbance Transmittance Concentration 13 Main Screen of A Trans Conc 13 Absorbance Transmittance Mode Concentration Standard Mode Concentration Factor Mode Standard Curve Main Screen of Standard Curve 19 Kinetics Main Screen of Kinetics Math Function for Kinetics Data import to Microsoft Excel UNICO Application Software Introduction The UNICO Application Software VIS1200 has been designed to operate with UNICO Spectrophotometer Model 1200 1201 The software runs on a PC personal computer with Windows 95 98 Me NT 2000 XP operating system installed Your UNICO Application Software package includes One CD containing the software VIS1200 Software User s Manual A 6 null modem connection cable with 9 pin and 25 pin female connector s on both ends The UNICO Application Software performs the following methods for analysis Absorbance Transmittance Concentration measure the Absorbance Transmittance Concentration
19. ure 22 Wavelength and Reference Setting Figure 23 TEST Button and Data Table Figure 24 Test Date 16 UNICO Application Software Tanna TETI DE Figure 25 Sample Test Results of Absorbance Transmittance Mode Concentration Standard Mode The purpose of this test is to determine the Concentration of the unknown samples by comparing the samples Absorbance Transmittance to that of the standard solution Repeat Step 1 to 3 in Absorbance Transmittance Mode section Step 4A Type in the known concentration of the standard solution in the Text Field at the right of Conc Std label Step 5A Place the standard in the Sample Compartment and close the lid Step 6A Click the OK Button in Test Parameter section This will measure the Absorbance of the standard and set its conversion Factor for measuring the unknown samples Figure 26 z Figure 26 Standard Solution Absorbance Measurement 17 UNICO Application Software Step 7A Place your sample s in the Sample Compartment and click the TEST Button for each sample to be measured Absorbance Transmittance and the Concentration of the samples will be displayed in the Data Table Figure 27 ER E si giae Tearoom cre e ox T m e nn Figure 27 Sample Test Results of Concentration Standard Mode Concentration Factor Mode The purpose of this test is to measure the Concentration of the sample
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