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1. Fundamentally the GenePix scanner uses a laser excitation based fluorescence scanning and imaging system The optical system can be considered in terms of excitation light mirrors lenses filters and photo detection The optical lens is located beneath the slideholder therefore the specimen must be placed feature side down into the slide holder GenePix Pro software automatically displays the image as if you were viewing the specimen from the top view i e the upper left corner of the slide is also the upper left corner in the acquired image Chapter 4 16 e Imaging System Hardware Excitation In the GenePix scanner standard configuration laser excitation is provided by individual 635 nm and 532 nm lasers These wavelengths correspond to the ideal wavelengths used to excite the fluorophores Cy3 and Cy5 Amersham Biosciences which are currently the most widely used fluorescent dyes for microarray imaging The individual lasers have been selected because of their superior optical performance and reliability Since the performance of such lasers is often sensitive to external temperature fluctuations the GenePix scanner uses an active temperature stabilization design to minimize temperature based laser fluctuations Furthermore each laser has stabilized feedback output to ensure that photon output is constant The GenePix Professional 4200A scanner can be configured with two additional lasers for a total of four installed laser light s
2. GenePix Pro software see below 7 Close Windows and turn off the computer Attach the SCSI cable to the SCSI adapter and the GenePix scanner the scanner should also be powered off see below 8 Turn on GenePix scanner first and wait for approximately 15 seconds before turning on computer If you already have a SCSI card installed on your host computer and do not want to replace it with the included SCSI card you must verify that it is a SCSI 2 device that 1s Win ASPI compliant You must also ensure that you have the proper cabling to connect the SCSI card to the GenePix scanner While it is possible that an existing SCSI card may work with the GenePix scanner technical support is provided only for the SCSI card that is bundled with the GenePix If you are using the GenePix scanner on a computer with multiple SCSI devices please ensure that there are no SCSI ID conflicts At the factory the GenePix scanner SCSI ID is set to 4 Installing the GenePix Pro Software To install the GenePix Pro software please consult the instructions in the GenePix Pro manual GenePix 4200A User s Guide Copyright 2005 Axon Instruments Molecular Devices Corp Installation e 7 Connecting the GenePix Scanner to the Computer Caution Failure to follow these instructions in the correct order can lead to damage to your instrument Before connecting the GenePix scanner to your SCSI connector please ensure that your computer has been turned off and
3. composant derri re le support des lames Ceci risquerait de provoquer une d faillance des syst mes de s curit syst mes double verrouillage Informations de S curit Importantes 44 e Informations de S curit Importantes Puissance et Classification des Lasers Utilis s Longueur d onde Puissance Divergence Dur e Classe des lasers embarqu s Classe Classe 0 92 mrad Classe Classe Illb Avertissement L exposition au rayonnement de laser peut entra ner des dommages corporels Par exemple fixer directement le rayonnement de laser entra ne un risque de c cit Danger de haute tension Des circuits haute tension se trouvent l int rieur de l instrument laser Microarray Scanner GenePix 4200A Dans des conditions d utilisation normales vous tes prot g e des hautes tensions l int rieur de l instrument Dans le cas peu probable o le tomographe re oive une surtension subite sup rieure 1 kilovolt il se peut qu il se remette z ro Pour continuer le fonctionnement normal remettez simplement en marche l ordinateur et le tomographe Avertissement Ne tentez pas d acc der l int rieur de l instrument L exposition des circuits haute tension peut provoquer des dommages corporels s v res ou fatals Il existe galement des circuits haute tension dans l ordinateur contr lant le scanner Reportez vous la documentation du fabricant de l ordinateur su
4. compromettre ses performances Temp rature 10 30 C Humidit 5 95 sans condensation Alimentation 100 240V 6A max 47 63 Hz Espace n cessaire 65 cm x 43 cm x 34 cm plus 15 2 cm 6 in clair Poids Structure suffisante pour supporter un poids de 45 kg 99 lbs pratiquement sans vibration GenePix 4200A User s Guide Copyright 2005 Axon Instruments Molecular Devices Corp Informaci n Importante sobre la Seguridad e 47 Informaci n Importante sobre la Seguridad Se asume que el operador del GenePix 4200A Microarray Scanner est entrenado en las precauciones de seguridad a seguir y en la utilizaci n correcta del instrumento La palabra usted como se utiliza en esta gu a del usuario del GenePix 4200A Microarray Scanner se refiere a este operador entrenado Declaraciones de Seguridad Utilizadas en esta Gu a Cercior se de observar las declaraciones de seguridad que se presentan en esta gu a Declaraci n de Seguridad L ser El Centro de Equipos de Salud Radiol gica CDRH siglas en Ingl s del Departamento de Administraci n de Drogas y Alimentos de los Estados Unidos ha implementado regulaciones para los productos de l ser fabricados antes del 1 de Agosto de 1976 El cumplimiento de las regulaciones es obligatorio para los productos vendidos en los Estados Unidos El esc ner est certificado como producto l ser Clase 1 bajo la Norma del Desempe o de Radiaci n del Departamento de Salud y Servic
5. have very sharp cut on and cut off frequencies so the transmission coefficient is above 0 8 within the entire specified band and close to zero outside it The standard emission filters are custom designed to reject laser light at optical density OD 8 for each respective laser excitation and reject broad spectrum light at OD 5 Chapter 4 18 e Imaging System Hardware Emission filters are located in a sixteen position filter wheel that is readily accessible in the GenePix Professional 4200A Four positions in the filter wheel are reserved for the standard emission filters red yellow green blue and are installed at the factory and are not accessible The remaining twelve positions are available to the user for custom filter installation see below Additional filters can be obtained directly from Axon or a custom filter can be ordered directly through a optical filter company such as Chroma Technology Corp Brattleboro VT Omega Optical Inc Brattleboro VT or Semrock Rochester NY We recommend that you use latex gloves or finger cots when handling filters to avoid getting fingerprints on the optical surfaces If you decide to use different dye and or filter combinations it is important to match the optical characteristics of filter dye and laser The transmission band of the filter should be centered as close as possible to the emission peak of the chosen dye and must be narrow enough to exclude light from the laser other
6. l instrument et entrainer un danger d exposition au rayonnement de laser de hautes tensions ou des pi ces mobiles Le GenePix 4200A est un instrument laser de Classe 1 Il incorpore des lasers de puissance auxquels l utilisateur ne peut pas acc der Le miroir mobile et le bloc optique situ s sous le porte lame focalisent ces faisceaux laser de classe 3B directement sur la face inf rieure de la lame d chantillons Les lames peuvent tre plac es en toute s curit dans la zone de balayage des lasers la porte coulissante bloque automatiquement l mission des rayons lors de son ouverture Il y a deux acc s potentiels sous l appareil qui ne poss dent pas cette protection ils sont GenePix 4200A User s Guide Copyright 2005 Axon Instruments Molecular Devices Corp Informations de S curit Importantes e 43 ferm s par des vis et requi rent donc l usage d outils pour leur ouverture voir les figures ci dessous N enlevez aucune vis pour tenter d ouvrir le bo tier ou pour aucune autre raison Les lasers internes peuvent endommager vos yeux avant que vous n ayez le temps de les fermer Attention Pr sence de hautes tensions l int rieur L utilisateur ne doit proc der aucune intervention l int rieur du bo tier L appareil peut tre endommag par une simple tentative d ouverture du bo tier Sympt mes Li s aux Probl mes de Verrouillage Il est dangereux de continuer utiliser le GenePix 42
7. s non verrouill s par la Double Protection Vue de l avant et de l arri re du GenePix 4200A montrant les sept vis qui maintiennent le bo tier NE PAS RETIRER CES VIS DANGER Radiation laser lorsque ouvert EVITER IMPERATIVEMENT L EXPOSITION DIRECTE AUX RAYONS Dont remove these screws Maintenance et Service des acc s non verrouill s par la Double Protection Aucune maintenance de la part de l utilisateur n est n cessaire De plus auun service de maintenance n est autoris de la part d un tiers Liste des Contr les Ce guide constitue une liste des contr les effectuer r guli rement Attention Tout contr le r glage ou ex cution de proc dures autres que celles sp cifi es ci dessus peut entra ner un risque d exposition dangereuse des rayonnements Etiquettes de s curit Si une tiquette devient illisible ou manque pour une raison quelconque contactez le service Support du Microarray System pour obtenir gratuitement une tiquette de remplacement En attendant copiez les tiquettes illustr es dans ce manuel et attachez cette copie l instrument Informations de S curit Importantes 46 e Informations de S curit Importantes Significations des Symboles Appareil en attente Pr t scanner une lame Attend en position de depart Balayage en cours Ejection Support de lame accessible This device complies with LIFTING HAZARD 21 CFR 1040 10 am
8. scanner has been designed to provide robust long lived microarray scanning for years of normal use However if the images you acquire on the GenePix scanner do not appear correct and if you believe there is a problem with the scanner it is important to follow these instructions before contacting Technical Support 1 Start the GenePix Pro software and click the Report tab 2 Click the Functional Checkout link 3 Follow the Functional Checkout procedure GenePix Pro automatically logs hardware performance into the Hardware Diagnostics Report every two hours during operation Installing Additional Filters Your GenePix 4200A Professional scanner comes with one standard filter for each installed laser The standard filters are not accessible by the user You can install additional filters in any of the twelve remaining filter positions The installation process involves two steps 1 Install the filters in the scanner 2 Record the information for the new filters in GenePix Pro GenePix 4200A User s Guide Copyright 2005 Axon Instruments Molecular Devices Corp Operation e 11 1 Install the filter s in the scanner Turn off power to the instrument before opening the filter wheel compartment 1 4 Use a 5 32 hex wrench provided with the optional filter kit to access the filter wheel compartment on the front of the scanner Figure 1 Turn the hex wrench counterclockwise to release the filter door latch Pull the h
9. that the GenePix scanner power is turned off Once the computer has been turned off connect the 50 pin data cable between the SCSI adapter of your computer and the GenePix scanner hardware The SCSI connector and power connector are located at the back of the GenePix scanner Once the SCSI connection has been made connect the power cord to the GenePix scanner Turn on the power supply and then turn on your computer Windows 2000 and Windows XP Installation If the connections have been made correctly and GenePix Pro software has already been installed when you restart your computer Windows will show a message that indicates that New Hardware has been detected Windows 2000 will automatically install the necessary driver for the GenePix scanner Windows XP requires that you manually install the driver using the Add New Hardware Wizard This wizard starts any time a new Plug and Play device is detected Working through the wizard select the option to Install the software automatically Recommended Press Next and then press Finish when prompted Repeat the procedure seven additional times If you are connecting the GenePix scanner to a computer that was purchased with the GenePix scanner the driver is already installed Chapter 2 8 e Installation Connecting the Security Key The GenePix Pro software requires a security key to operate If the key is absent you will be able to open images in GenePix Pro but you will not b
10. the top half With one finger hold open the flat spring positioner on the inside left edge of the slide holder Drop the slide face down into the slide holder frame The slide should be supported by the three sapphire balls on the frame A slight flick of the flat spring positioner will ensure proper positioning of the slide Do not push the slide into position Gently close the slide holder lid and close the door You are now ready to scan your sample See Image Acquisition in the Tutorial section of the GenePix Pro User s Guide for scanning instructions Note The slide holder is a precision component designed to ensure proper focusing and field uniformity Improper handling may damage the slide holder and affect imaging performance Never touch the slide holder while it is moving Never force the slide holder closed or apply significant pressure to it for any reason Do not rub or scratch the sapphire balls Chapter 3 10 e Operation Functional Checkout The Functional Checkout procedure is to help you confirm that your GenePix Professional 4200A microarray scanner is performing according the specifications set by Axon Instruments Inc A similar but much more detailed procedure was performed at the factory prior to shipping this unit to you We recommend that you follow the procedure once upon receiving the instrument and then any time after that if you wish to check the performance of the instrument The GenePix 4200A microarray
11. 00A si seul l un des deux verrouillages de s curit fonctionne Pri re de contacter Axon Instruments imm diatement l un des num ros indiqu s l arri re du GenePix 4200A L apparition de l un des sympt mes suivants d signe un probl me avec la double protection 1 L ensemble miroirs lentilles continue se d placer sous la lame apr s l ouverture de la porte coulissante 2 La DEL Scanning voyant bleu l avant du GenePix 4200A ne s teint pas quand la porte est ouverte 3 Il vous est impossible de scanner une lame 4 Vous n entendez plus le bruit m tallique caract ristique que provoque le verrouillage m canique lorsqu il est enclench Ce clic s entend normalement d s que la porte coulissante est ouverte d un quart de pouce environ 6 mm Attention danger en soulevant Avertissement Le GenePix Professional 4200 Scanner p se peu pr s 45 kg 99 Ibs Il faut 2 personnes pour le soulever Il y a 4 poign s en dessous du scanner voir le diagram Utilisez la technique correcte pour le soulever pour eviter des risques de blessure CLASS 1 LASER S curit concernant les Lasers PRODUCT Pr cautions prendre Ne rien introduire dans les ouvertures ou a rations du GenePix 4200A part une lame de microscope dans le compartiment pr vu pour son chargement Vous pourriez provoquer une d faillance des syst mes de s curit syst mes double verrouillage Ne pas tenter de d placer un
12. 05 Axon Instruments Molecular Devices Corp 6 7 Operation e 13 In the Filter Tool program click the checkbox for the filter position you are adding Enter the midpoint of the wavelength range that is transmitted by the filter For example a 530DF30 filter transmits a 30 nm band of light centered at 530 nm i e it transmits 515 545 nm therefore the midpoint of this filter is entered as 530 For a long pass filter enter the peak emission of the dye you will use with that filter In GenePix Pro you can display either the filter s Formal name e g S30DF30 or an Alternate name e g Alexa 488 or Blue Filter Enter both names here so that each user can choose their preferred nomenclature in GenePix Pro After you have entered this information for each new filter click Save Close the Filter Tool program When you open GenePix Pro the new filters are listed in the Hardware Settings dialog box Chapter 3 Imaging System Hardware e 15 Chapter 4 Imaging System Hardware The GenePix Professional 4200A scanner is one of the most sensitive scanners currently available This chapter introduces some of the fundamental hardware concepts needed to understand the operation of the GenePix scanner Discussion of Hardware Components When discussing the hardware components of the GenePix scanner it is convenient to reduce the system to its optical mechanical and electrical components Optics
13. 33 6 545 264 6 597 000 6 643 015 and 6 650 411 GenePix 4200A User s Guide Copyright 2005 Axon Instruments Molecular Devices Corp Declaration of Conformity e 35 Declaration of Conformity Manufacturer Molecular Devices Corp Axon Instruments 3280 Whipple Road Union City CA 94587 USA Type of Equipment Microarray Scanner Model Number GenePix Professional 4200A Serial Number Year of Manufacture 2002 Application of Council Directives EC EMC Directive 89 336 EEC as amended EC Low Voltage Directive 73 23 EEC as amended Harmonized Standards to which Conformity is Declared EMC EN 61326 04 1997 Emissions EN 61326 04 1997 Immunity Low Voltage EN 61010 1 1993 A1 A2 1995 EN 60825 1 1994 A11 1996 Laser Safety 1 the undersigned hereby declare that the equipment specified above conforms to the above Directives and Standards Authorized Signature and Date _ Signature on file Declaration of Conformity Important Safety Information e 37 Important Safety Information The operator of the GenePix Professional 4200A Microarray Scanner instrument is assumed to be trained in the correct operation of the instrument and the safety issues Throughout the GenePix Professional 4200A Microarray Scanner User s Guide the word you refers to this trained operator Safety Text Used In This Guide Make sure you follow the precautionary statements presented in this guide Laser Safety Statement Th
14. ER SEE California 94587 U S A THE PROTECTION PROVIDED Gebrauchsanmeisunig Tel 1 510 675 6200 BY THE EQUIPMENT MAY BE IMPAIRED Fax 1 510 675 6300 rtliche Voraussetzungen Der GenePix 4200A arbeitet optimal wenn alle Umfeldvorrausetzungen gegeben sind Genau wie bei anderen optischen Ger ten sollte auch hier ein staub und schwingungsfreies Umfeld existieren Extreme Temperaturen und Luftfeuchtigkeit k nnen die optimalen Funktionen des Ger tes beeintr chtigen Umgebungstemperatur 10 30 C Umgebungsluftfeuchtigkeit 5 95 unkondensiert Stromvoraussetzung Zufuhr 100 240 V 6 A max 47 63 Hz Platzvoraussetzung 65 cm x 43 cm x 34 cm Bitte einen zus tzlichen Abstand von 15 2 Zentimeter 6 Zoll frei zu lassen Gewichtsvoraussetzung Genug um 45 kg 99 Pfund zu unterst tzen mit minimalen Schwingu GenePix 4200A User s Guide Copyright 2005 Axon Instruments Molecular Devices Corp Alexa Fluor Dyes 16 Amplifiers 22 Custom Filters 18 Cy3 Cy5 16 Digitization 1 Emission Filter 17 18 Filters Installing 10 Fluorophore 17 See Cy3 Cy5 GenePix Installation 3 Hardware 15 Amplifiers 22 Laser Excitation 16 Index e 57 Index Quantum Efficiency 20 Help On Line 3 Hardware Spatial Resolution 22 Lasers Temperature Fluctuations 16 On line Help 3 Photo Multiplier Tube Gain 20 Pixel Size 22 23 24 Preview Scan 23 24 Spatial Resolution 22 23 Stokes Sh
15. GenePix Professional 4200A MICROARRAY SCANNER User s Guide Part Number 2500 0153 Rev August 2005 Printed in USA Copyright 2005 Axon Instruments Molecular Devices Corp No part of this manual may be reproduced stored in a retrieval system or transmitted in any form or by any means electronic mechanical photocopying microfilming recording or otherwise without written permission from Molecular Devices Corp QUESTIONS See Axon s Knowledge Base http support axon com PLEASE READ SAFETY There are important safety issues that you must take into account when using this instrument Please carefully read the safety warnings starting on page 37 before you use this instrument VERIFICATION This instrument is extensively tested and thoroughly calibrated before leaving the factory Nevertheless researchers should independently verify the basic accuracy of the instrument using suitable test samples WARNING If this equipment is used in a manner not specified by the manufacturer the protection provided by the equipment may be impaired DISCLAIMER This equipment is not intended to be used and should not be used in human experimentation or applied to humans in any way Verification Warning Disclaimer Table of Contents e iii Table of Contents Chapter T Introduction eoe toe tetro eo eon orba onu noeh orta ds ina eT os ere 1 Chapter 2 Installations est oon teo eee oen thao ea kk rn
16. Hochspannungsgefahr Der GenePix 4200A Microarray Scanner enth lt Hochspannungselektronik Unter normalen Gebrauchskonditionen sind Sie vor diesen Spannungen gesch tzt Im unwahrscheinlichen Fall das Skanner eine Energieschwankung gr sser als 1 kV bekommt kann das Skanner zur ckstellen Zu funktionieren weiter einfach drehen Sie beiden der Computer und das Skanner weg und dann an wieder Warnung Versuchen Sie nicht an das innere Teil des Scanners heranzukommen Kontakt mit der Hochspannungselektronik kann schwere oder t dliche Verletzungen verursachen Hochspannungselektronik befindet sich auch im Kontrollrechner des Ger tes Lesen Sie sich bitte die Gebrauchsanweisung ber die Hochspannungsgefahr durch Befolgen Sie alle Anweisungen ber die Sicherheitsmassnahmen beim Gebrauch des Rechners Bewegliche Teile Der GenePix 4200A Microarray Scanner enh lt bewegliche Teile die Verletzungen verursachen k nnen Unter normalen Umst nden ist das Ger t so konstruiert um Sie vor den beweglichen Teilen zu sch tzen Die Verrieglung und das Schiitzgeh use sind da damit ein Zugriff auf die beweglichen Teile w hrend einer Ablesung nicht m glich ist Warnung Versuchen Sie nicht das innere Teil des Ger tes zu ffnen Die darin enthaltenen beweglichen Teile k nnen Verletzungen verursachen Sicherung Es gibt zwei Sicherungen 2A 250V die neben dem Schalter der Stromversorgung an der R ckseite des Ger tes gelegen sind Sicherheitsvork
17. RAGED TO EVALUATE WHETHER A LICENSE IS REQUIRED FOR YOUR SPECIFIC APPLICATION OF THIS INSTRUMENT COMPANIES THAT HAVE INTELLECTUAL PROPERTY RIGHTS IN THE POTENTIAL FIELD OF APPLICATION OF THIS INSTRUMENT INCLUDE WITHOUT LIMITATION AFFYMETRIX INC Licensing Notice 34 e Licensing Notice AFFYMETRIX AGILENT AND OXFORD GENE TECHNOLOGY THIS INSTRUMENT HAS NOT BEEN LICENSED OR APPROVED FOR DIAGNOSTIC APPLICATIONS THE USE OF THIS INSTRUMENT IN CONNECTION WITH MICROARRAYS MAY BE WITHIN THE SCOPE OF PATENTS HELD BY AFFYMETRIX TO THE EXTENT THAT AFFYMETRIX PATENT RIGHTS ENCOMPASS THIS INSTRUMENT OR ITS USE AFFYMETRIX HAS GRANTED A LIMITED PATENT LICENSE FOR RESEARCH USE ONLY AND NOT FOR USE IN DIAGNOSTIC PROCEDURES SUCH LICENSE IF APPLICABLE IS LIMITED TO USE OF THIS INSTRUMENT WITH SPOTTED MICROARRAYS SEPARATELY LICENSED BY AFFYMETRIX NO LICENSE IS CONVEYED BY IMPLICATION ESTOPPEL OR OTHERWISE TO USE THIS INSTRUMENT WITH MICROARRAYS MADE USING N SITU OR PHOTOLITHOGRAPHIC SYNTHESIS NO OTHER LICENSE IS CONVEYED BY IMPLICATION ESTOPPEL OR OTHERWISE UNDER ANY AFFYMETRIX PATENT OR OTHER INTELLECTUAL PROPERTY RIGHT This instrument is licensed by Affymetrix under the following patents U S Patent Nos 5 578 832 5 631 734 5 834 758 5 936 324 5 981 956 6 025 601 6 141 096 6 171 793 6 185 030 6 201 639 6 207 960 6 218 803 6 225 625 6 252 236 6 262 838 6 335 824 6 403 320 6 403 957 6 407 858 6 472 671 6 490 5
18. a puede ocasionar da o en los componentes del instrumento y producir exposici n accidental a la radiaci n l ser al alto voltaje o a partes m viles El GenePix 4200A es un equipo de l ser de Clase 1 Adentro se localizan l seres de alta potencia fuera del alcance del usuario El espejo movible y el conjunto de lentes localizados debajo del portaobjeto tambi n laminilla o platina dirige y enfoca los rayos l ser 3B directamente hacia arriba a la superficie de la laminilla de muestra Las placas pueden colocarse sin peligro en la regi n del area de barrido del l ser porque la entrada de las placas tiene circuitos de seguridad redundantes que bloquean los rayos de luz Hay dos paneles en el equipo que no son Informaci n Importante sobre la Seguridad 48 e Informaci n Importante sobre la Seguridad parte del circuito de seguridad pero que est n asegurados con tornillos que requieren una herramienta para sacarlos Estos est n indicados mas adelante No remueva ning n tornillo para abrir la cubierta Los l seres interiores pueden da ar sus ojos antes de que pueda pesta ear Interiormente hay altos voltajes En el interior no hay partes que requieran servicio por parte del usuario La unidad se da ar si usted intenta abrir la cubierta protectora S ntomas de Da os en el Circuito de Seguridad Si usted encuentra cualquiera de los siguientes s ntomas el circuito de seguridad podr a estar da ado Es peligroso contin
19. act the supplier for current rates or see our web site at www axon com Optional Service Agreement Purchasing an optional Service Agreement extends the coverage of the Standard Warranty Contact the supplier for current rates Shipping The GenePix 42004 is a solidly built instrument designed to survive shipping around the world However in order to avoid damage during shipping the GenePix 4200A must be properly packaged In general the best way to package the GenePix 4200A is in the original factory carton If this is no longer available we recommend that you carefully wrap the GenePix 4200A User s Guide Copyright 2005 Axon Instruments Molecular Devices Corp Warranty and Repair Service e 31 GenePix 4200A in at least three inches 75 mm of foam or bubble pack sheeting The wrapped instrument should then be placed in a sturdy cardboard carton Mark the outside of the box with the word FRAGILE and an arrow showing which way 1s up We do NOT recommend using loose foam pellets to protect the GenePix 4200A During shipping there is a good chance that the instrument will shift within the loose pellet packing and be damaged If you need to ship the GenePix 4200A to another location or back to the factory and you do not have a means to adequately package it Axon Instruments can ship the proper packaging material to you for a small fee This may seem an expense you would like to avoid but it is inexpensive compared to the cos
20. andard laboratory procedures and cautions when working with chemicals Caution Always follow the manufacturer s precautions when working with chemicals Axon Instruments is not responsible or liable for any damages caused by or as a consequence of the use of any hazardous material Important Safety Information 40 e Imporrtant Safety Infomation Non Interlocked Panels View of the top and rear of GenePix Professional 4200A scanner showing the twenty screws that attach the protective housing DO NOT REMOVE THESE SCREWS DANGER LASER RADIATION WHEN OPEN AVOID DIRECT EXPOSURE TO BEAM Don t remove these screws Maintenance and Service No user maintenance is required No user service is allowed If there is a problem please contact Technical Support List of Controls This Guide constitutes a list of controls Caution Use of controls or adjustments or performance of procedures other than those specified herein may result in hazardous radiation exposure Safety Labels If a label becomes illegible or is missing for any reason please contact Microarray System Technical Support for a free replacement label While waiting for the replacement label copy the label shown in this section and attach the copy of the label to the instrument GenePix 4200A User s Guide Copyright 2005 Axon Instruments Molecular Devices Corp Important Safety Information e 41 Symbol Explanations gt Device standby Sca
21. andle to remove the filter cartridge Figure 2 Figure 1 Opening the filter compartment Figure 2 Removing the filter cartridge Turn the wheel to align any open position with the hole in the top of the filter cartridge The number of that filter position is visible in the smaller adjacent hole Note the number of the filter position you will use this number must be recorded in GenePix Pro see below Handle filters by the edges only The rim of the filter is marked with an arrow indicating the direction of light propagation through the filter Drop the filter into the hole with the arrow pointing down Using the cylindrical wrench screw a lock ring into the hole to gently secure the filter Do not over tighten Figure 3 Tightening the lock ring Chapter 3 12 e Operation 5 Repeat for any additional filters 6 When you have installed all additional filters insert the filter cartridge into the instrument Close the door and turn the hex key clockwise to secure the latch 2 Record the information for the new filters in GenePix Pro 1 Turn on the scanner and computer If GenePix Pro is running shut it down 2 From the Windows Start menu go to Programs Axon Laboratory GenePix Pro Filter Tool Filter Tool for GenePix 4200 V1 0 0 7 Jv Filter 7 a Bor o Alexa 488 2 zu Iv Ei Bi z Bi ml T GenePix 4200A User s Guide Copyright 20
22. ck through the optical system where they pass through another set of bandpass filters see below before reaching the photodetector Emission Filters Light from the slide is filtered before reaching the photodetectors to ensure that as far as possible the detectors measure only emission photons from the appropriate fluorescent dye To achieve this the filter s window is matched as closely as possible to the peak emission spectrum of the selected dye to maximize acceptance of the desired emission light and at the same time exclude stray laser excitation light and light emitted by non target fluorescence autofluorescence reagent background etc The GenePix scanner comes with standard emission filters installed one for each laser installed at the time of purchase Each standard emission filter is optimized for the emission spectrum of the respective dye The factory installed Cy5 red filter is a bandpass filter with cut on frequency at a wavelength of 655 nm and cut off frequency at 695 nm This is ideal for selecting the emission photons of the red dye Cy5 which has an emission spectrum centered on 667 nm and excluding light from the 635 nm laser and the Cy3 dye Likewise the Cy3 green filter transmits 550 600 nm light the standard yellow emission filter transmits 607 695 nm light and the standard blue filter transmits 508 560 nm The spectra for the standard emission filters in the GenePix scanner
23. dende T r mehrere Verriegelungen hat die Laserstrahlen blockieren Es gibt zwei Offnungen die nicht elektrisch verriegelt sondern mit Schrauben befestigt sind Diese k nnen nur mit Hilfe eines Werkzeuges entfernt werden Sie sind unten abgebildet Entfernen Sie niemals die Schrauben um das Geh use zu ffnen GenePix 4200A User s Guide Copyright 2005 Axon Instruments Molecular Devices Corp Sicherheitsmassnahmen e 53 Die im Ger t enstehenden Laserstrahlen k nnen Ihre Augen besch digen bevor Sie Gelegenheit haben sich zu schiitzen Im Ger t entstehen hohe Spannungen Es befinden sich im Ger t keine f r den Nutzer justierbaren Teile Wenn Sie versuchen das Schutzgeh use zu ffnen kann dies eine Besch digung des Ger tes zur Folge haben Hinweise auf ein Versagen der elektrischen Verriegelung Wenn eines der nachfolgenden Probleme auftritt hat wahrscheinlich eine Verriegelung versagt Es ist sehr gef hrlich weiterhin den GenePix 4200A zu benutzen wenn nur eine elektrische Verriegelung funktioniert Bitte rufen Sie sofort den Technischen Hilfsdienst an sobald eines der unten beschriebenen Symptome auftritt Die Service Nummern befinden sich auf der hinteren Seite des Ger tes 1 Spiegel und Linsensysteme bewegen sich weiterhin unter dem Objekttr ger nachdem die T r ge ffnet wurde 2 Die blaue Scanning Lichtdiode an der Vorderseite des GenePix 4200A erl scht nicht wenn die T r ge ffnet wird 3 S
24. ders of magnitude Rotational Uniformity Less than 4 non uniformity Dimensions 43 cm 16 9 x 65 cm 25 6 x 34 cm 13 4 WxDxH Weight 45 kg 99 Ib Power supply Line voltage 85 to 264 VAC 110 to 340 VDC universal voltage input Line frequency 50 60 Hz Power 150 W Fuse 2 0 A slow 5 x 20 mm GenePix 4200A User s Guide Copyright 2005 Axon Instruments Molecular Devices Corp Technical Assistance e 27 Technical Assistance If you need help to resolve a problem there are several ways to contact Axon Instruments Molecular Devices World Wide Web WWW axon com Phone 800 635 5577 Fax 1 510 675 6300 E mail axontech axon com Questions See Axon s Knowledge Base http support axon com Technical Assistance Warranty and Repair Service e 29 Warranty and Repair Service Standard Warranty Axon Instruments Molecular Devices Corp warrants its non consumable hardware products to be free from defects in materials and workmanship for 12 months from date of invoice or date of purchased installation visit which ever is later The warranty covers the cost of parts and labor to repair the product Please keep the shipping container for future use If you should need an additional container one can be provided for an additional charge Products returned to our factory for repair should be properly packaged with transportation charges prepaid Axon Instruments Molecular Devices will pay for the ret
25. dyes etc but not so narrow that too many emission photons are excluded otherwise sensitivity will be lost We also strongly recommend that a reject filter of OD 8 be employed to eliminate laser excitation Installing Filters Before installing filters turn the scanner power off To gain access to the filter wheel use the hex key to open the front access panel and gently pull the filter wheel cassette out by the handle The filter wheel cassette is numbered and there is an access port on the top of the cassette in which to install filters There is an opening at the rear of the cassette that allows you to rotate the filter Choose a filter position by moving the filter wheel so that the designed position is lined up with the top access port There is an arrow on the mounting ring of the filter that indicates the propagation direction The arrow on all filters obtained from Axon Instruments indicate the propagation path to the PMT detector thus the arrow should be facing down into the table when installing a filter in GenePix 4200A User s Guide Copyright 2005 Axon Instruments Molecular Devices Corp Imaging System Hardware e 19 the filter wheel Different manufacturers have different conventions so if you obtain a filter from a third party consult with the manufacturer s documentation in order to determine the correct orientation Once you determine the orientation of the filter carefully drop it into position a small hex key o
26. e Center for Devices and Radiological Health CDRH of the U S Food and Drug Administration has implemented regulations for laser products manufactured since August 1 1976 Compliance is mandatory for products marketed in the United States The scanner is certified as a Class 1 laser product under the U S Department of Health and Human Services DHHS Radiation Performance Standard according to the Radiation Control for Health and Safety Act of 1968 Since radiation emitted inside the scanner is completely confined within protective housings and external covers the laser beam cannot escape during any phase of normal user operation Warning Using controls making adjustments or performing procedures other than those specified in the GenePix 4200A User s Guide may result in hazardous exposure to laser radiation high voltage or moving parts Exposure to these hazards can cause severe or fatal injury Caution Indicates that damage to the instrument loss of data or invalid data could occur if the user fails to comply with the advice given Important Highlights information that is critical for optimal performance of the system Note Identifies items of general interest The Protective Housing The protective housing and instrument interlocks are designed to protect you from exposure to the laser radiation high voltage or moving parts You do not need to access the interior of the instrument There are no serviceable parts inside Warn
27. e able to save your settings files or save your analysis results In addition you can analyze only 1000 features without a key To install the GenePix Pro key please consult the instructions in the GenePix Pro manual Troubleshooting Installation SCSI Card Installation Problems If you followed the above instructions and still require further assistance on installation of the included SCSI card contact Technical Support If you are using SCSI card that was not provided with your GenePix scanner you should contact the supplier of that SCSI card for technical support GenePix Pro automatically detects the GenePix Professional 4200A scanner If this does not occur please verify the following items e The data cable between the computer and the scanner is securely connected e The GenePix scanner has power the green power light at the front of the scanner will be glowing e The SCSI card is operational see above e The GenePix SCSI ID 4 does not conflict with any other SCSI devices that you may have already installed on your computer e g a Jaz drive e The scanner power was turned on before booting the computer GenePix 4200A User s Guide Copyright 2005 Axon Instruments Molecular Devices Corp Operation e 9 Chapter 3 Operation Inserting a Slide To scan a slide first lift the door The slide holder will move to its forward most position for loading Gently lift the tab on the front of slide holder to raise
28. e ee eS beoe ens 3 About This User s Guide mociones aa 3 Computer Requirements eiii tibt Ge EL 3 Setting Up and Moving the GenePix scanner esee 4 Preparing to Install the GenePix Scanner 4 Quick Overview of Hardware and Software Installation sesss Installing the SCSI irn 8 in LE tita 5 Installing the GenePix Pro Software 6 Connecting the GenePix Scanner to the Computer 7 Windows 2000 and Windows XP Installation esee 7 Connecting the Security Key eden etie e et Len 8 Troubleshooting Installation ss 8 SCSI Card Installation Problems 8 Chapter 3 Operation lt csissccicssrsccersssesvessonessendesenssssenscsevectessessestctessesesesdesssedssesbesesses 9 Inserting a Slide ioni ree eret dere epo Modes net etna 9 Functional Checkout cierta c de eee rre date 10 Installing Additional Filters enne 10 Chapter 4 Imaging System Hardware ecce ecce esee eee eerte ee eee seen 15 Discussion of Hardware Components 15 OPUCS miii ee A et tei tt ete dnd ede 15 Mechanical ito nime nomades metae 20 ElECtEONI iier e 21 General Items of Interest u re es eisen be 22 Spatial Resolution iiec in le ir 22 Table of Contents iv e Table of Contents Dynamic Range and Detection Limit 25 Speci Cations o ios ote oid
29. e rayonnement mis l int rieur du tomographe est compl tement confin dans les bo tiers protecteurs et les couvertures externes le rayon laser ne peut pas s chapper pendant une phase de fonctionnement normal Avertissement L utilisation de commandes et l ex cution de r glages ou de proc dures autres que ceux sp cifi s dans le GenePix 4200A User s Guide entraineraient de graves dangers d exposition au rayonnement de laser des hautes tensions ou des pi ces mobiles Ces dangers peuvent tre l origine de dommages corporels s v res ou fatals Attention Indique un risque de dommage pour l instrument de perte de donn es ou de donn es invalides si l utilisateur ne suit pas les recommandations de ce manuel Important D signe des informations vitales pour le fonctionnement optimal du syst me Note Identifie des informations d int r t g n ral Le boitier de protection Le bo tier et les verrouillages de s curit de l instrument sont con us pour vous prot ger du danger d exposition au rayonnement de laser des hautes tensions ou des pi ces mobiles Il n est pas n cessaire que vous acc diez l int rieur de l instrument o aucune intervention n est n cessaire Avertissement Ne d sactivez pas les verrouillages de s curit n ouvrez pas le boitier et ne tentez pas d acc der l int rieur de l instrument par aucune autre ouverture L ouverture du bo tier pourrait endommager les composants de
30. ehrungen Der Benutzer muss alle normalen Laboranweisungen und Sicherheitsvorkehrungen befolgen wenn Chemikalien verwendet werden Achtung Befolgen Sie immer die Anweisungen der Hersteller wenn Sie mit Chemikalien arbeiten Axon Instruments bernehmen keine Verantwortung f r Sch den die durch den Missbrauch von gef hrlichen Chemikalien verursacht werden GenePix 4200A User s Guide Copyright 2005 Axon Instruments Molecular Devices Corp Sicherheitsmassnahmen e 55 Ger te ffnungen Ohne Elektrische Verriegelung Ober und R ckseitenansicht des GenePix 4200A zeigt die sieben Schrauben die das Sch tzgeh use befestigen ENTFERNEN SIE DIESE SCHRAUBEN UNTER KEINEN UMST NDEN VORSICHT Laserbestrahlung wenn ge ffnet VERMEIDEN SIE DIREKTEN KONTAKT MIT DEM LASERSTRAHL Don t remove these screws Instandhaltung und Vorsorge Es ist keine Vorsorge durch den Benutzer n tig Jede eigenm chtige Reparatur durch den Benutzer ist untersagt Wenn ein Problem auftritt dann rufen Sie bitte den Technischen Hilfsdienst an Liste von Kontrollvorrichtungen Diese Gebrauchsanweisung enth lt eine Liste der Kontrollvorrichtungen Achtung Der Gebrauch von Kontrollvorrichtungen oder Einstellungen bzw die Ausf hrung von Handlungen auBer denen die in dieser Anweisung beschrieben sind k nnen zu gef hrlichen Strahlungen f hren Sicherheitsetiketten Sollte ein Etikett unlesbar werden oder aus unbekannten Gr nden fe
31. esolution you will decrease the final image size in megabytes However keep in mind that you need to acquire enough pixels for each feature in order to make accurate measurements For a Preview Scan the same 5 um spots are scanned However the y direction step size is increased to 40 um and each 40 um Preview Scan pixel is an average of eight contiguous 5 um spots in the x direction This speeds image acquisition for balancing the PMT gains while minimizing photobleaching A 5 um Data Scan B 10 um Data Scan C 20 um Data Scan Chapter 4 24 e Imaging System Hardware E Preview Scan 40 um Representation of how the GenePix scanner samples 5 um spots and averages them to calculate the values of the image pixels for the various scan modes and pixel sizes On the left side of each image pair is the raw data scan from the GenePix Scanner Each square represents a 5 um x 5 um spot as scanned by the 5 um laser beam On the right are the resultant image pixels at various resolutions The shading indicates the spots that are scanned by the GenePix scanner as well as the corresponding pixels in the final image GenePix 4200A User s Guide Copyright 2005 Axon Instruments Molecular Devices Corp Imaging System Hardware e 25 Dynamic Range and Detection Limit The dynamic range of an instrument is the signal width that can be resolved above the noise the instrument produces The dynamic range depends on all the components in a
32. evice complies with LIFTING HAZARD 21 CFR 1040 10 8 1040 11 HEAVY OBJECT FUSE E T 250V T2A POWER FREQUENCY 100 240V 50 60Hz N NINE Atenci n MAX OPERATING CURRENT 1 25 AMP voltajes grosos SERIAL NUMBER LABEL NO CUSTOMER SERVICEABLE COMPONENTS ARE CONTAINED WITHIN THIS UNIT CONSULT THE MANUFACTURER FOR REPAIR RETURN INSTRUCTIONS THE USER SHALL BE MADE AWARE THAT Atenci n Axon Instruments Inc IF THE EQUIPMENT IS USED IN ANY MANNER netics 3280 Whipple Road Union City NOT SPECIFIED BY THE MANUFACTURER veal CONES para California 94587 U S A THE PROTECTION PROVIDED su uso Tel 1 510 675 6200 BY THE EQUIPMENT MAY BE IMPAIRED Fax 1 510 675 6300 Requerimientos del Lugar de Instalaci n El GenePix 4200A est dise ado para operar ptimamente si el lugar de instalaci n cumple con los requerimientos Como con cualquier instrumento ptico de precisi n debe tener cuidado de manterlo en un lugar bajo en polvo y de poca vibraci n Temperaturas extremas y humedad pueden comprometer su funcionamiento Temperatura ambiental 50 86 F 10 30 C Humedad ambiental 5 95 sin condensaci n Energ a requerida Entrada 100 240V 6 A m ximo 47 63 Hz Espacio requerido 25 6 profundidad x 16 9 amplitud x 13 4 altura 65 cm x 43 cm x 34 cm mas 6 pulgadas 15 2 cm adicionales de espacio libre Peso requerido Suficiente para sostener 45 kg 99
33. given system For example if you use a PMT with a dynamic range of 10 but process the signals through a sub standard 8 bit analog to digital converter you will end up with an instrument with a poor dynamic range Furthermore while an instrument may have a large dynamic range one may not be able to use the whole range reliably if the response is non linear Therefore when rating an instrument one must consider the range over which its response remains linear For the GenePix scanner the dynamic range is 10 The dynamic range of an instrument is often considered in conjunction with its detection limit The detection limit is defined as the dye concentration for which the signal to noise ratio 3 Signal to noise ratio is calculated as Signal Background Standard Deviation of Background Although you may be able to see features below this level the quantitative accuracy diminishes significantly Specifications Sample type Standard microscope slides 1 x 3 25 x 75 mm 26 x 76 mm 0 9 1 2 mm thick Maximum scan area 22x 73 mm Maximum resolution 5 Scan mode and order Sequential fully user configurable scan order Detector Photomultiplier tube PMT Digital resolution 16 bit Image format Single image or multi image TIFF Computer interface SCSI adapter included Scan time Four minutes per channel for full size scan area at 10 um resolution Chapter 4 26 e Imaging System Hardware Dynamic range Four or
34. hlen setzen Sie sich bitte mit dem Technischen Hilfsdienst f r einen kostenlosen Ersatz des Etiketts in Verbindung W hrend Sie auf das Etikett warten kopieren Sie die Etiketten am Ende dieses Abschnitts und befestigen Sie eine Kopie des Etiketts am Ger t Sicherheitsmassnahmen 56 e Sicherheitsmassnahmen Erkl rung Der Symbole ULL Standby Modus Scanning Eject Ger t ist bereit einen Objekttr ger zu lessen Ger t ist bereit zur Ausgabe des Objekttr gers F hrung des Objekttr gers in Startposition UR CAUTION GenePix PROFESSIONAL A du Achtung Axon ee i 4200A when ES js ie Gefahr bai Anhebung in 5cks defeated PATENTS PENDING Achtung CLASS 1 LASER PRODUCT This device complies with LIFTING HAZARD 21 CFR 1040 10 amp 1040 11 HEAVY OBJECT FUSE E T 250V T2A Laserbestrahlung HAZARDOUS VOLTAGE To prevent electric shock and damage to the instrument do not attempt PONER to open unit FREQUENCY 100 240V 50 60Hz Achtung MAX OPERATING CURRENT 1 25 AMP Gefahrlicher SERIAL NUMBER LABEL Stromkreis NO CUSTOMER SERVICEABLE COMPONENTS ARE CONTAINED WITHIN THIS UNIT CONSULT THE MANUFACTURER FOR REPAIR RETURN INSTRUCTIONS Achtung MADE IN TSA THE USER SHALL BE MADE AWARE THAT Vor Benutzung 7 IF THE EQUIPMENT IS USED IN ANY MANNER unbedingt de hipple Road Union City NOT SPECIFIED BY THE MANUFACTUR
35. ie k nnen keine Glasstreifen ablesen 4 Der Benutzer h rt nicht mehr das metallene Ger usch welches bei normaler Funktion durch das Einrasten der mechanischen Verriegelung ausgel st wird Dies passiert normalerweise wenn die T r ungef hr 6 mm ge ffnet ist Warnung Gefahr bei Anhebung Der Scanner GenePix Professional 4200 wiegt ungef hr 45 km 99 Pfund Zum Anheben des Scanners sind zwei Personen erforderlich Es gibt vier geformte Griffrohre auf der Unterseite des Ger tes siehe Abbildung Verwenden Sie eine r ckenschonende Hebetechnik um Verletzungen zu vermeiden CLASS 1 LASER Lasersicherheit PRODUCT Achtung Mit Ausnahme des Objekttr gers d rfen unter keinen Umst nden Gegenst nde in die ffnungen oder Ausg nge des GenePix 4200A gesteckt werden da sonst ein Versagen der elektrischen Verriegelung verursacht werden k nnte Unterhalb des Objekttr gers d rfen keine Teile bewegt werden auch dies k nnte ein Versagen der elektrischen Verriegelung verursachen Sicherheitsmassnahmen 54 e Sicherheitsmassnahmen Klassifizierung und Intensit t der benutzten Laser Wellenl nge St rke Divergenz Betriebsart Klassifizierung Kontinuierlich Klasse 1424 mW Kontinuierlich Klasse Illb 0 92 mrad Kontinuierlich Klasse Kontinuierlich Klasse Warnung Verletzungen k nnen enstehen wenn man Laserstrahlen ausgesetzt wird Direkter Kontakt mit den Strahlen kann zu Blindheit f hren
36. ift 17 Temperature Fluctuations 16 Index
37. ing Do not defeat the interlock open the protective housing or try to gain access to the interior of the instrument through any other openings Opening the housing can damage the instrument components and result in hazardous exposure to laser radiation high voltage or moving parts The GenePix 4200A is a Class I laser device There are high power lasers embedded inside it but the user cannot access these The moving Mirror and Lens Assembly located under the Slide Holder directs and focuses these Class 3B laser beams directly up onto the surface of the sample slide Slides can be placed safely in the laser s scanning area because the slide loading door has redundant interlocks that block the beams There are two panels on the device that are not interlocked but these are fastened with screws and require a tool to remove them They are shown in the Non Interlocked Panels section below Do not remove any screws to open the housing Important Safety Information 38 e Imporrtant Safety Infomation The lasers inside can cause eye damage before you have a chance to blink There are high voltages inside There are no user serviceable parts inside The unit will be damaged if you attempt to open the protective housing Interlock Failure Symptoms If you experience any of the following symptoms you may have an interlock failure It is unsafe to continue to use the GenePix 4200A with only one interlock functioning Please contact Technical S
38. ion can cause injury For example viewing the laser radiation directly can cause blindness High Voltage Hazard High voltage electronics can be found in the GenePix 4200A Microarray Scanner instrument Under normal operating conditions you are protected from high voltage within the instrument In the unlikely event the scanner receives a surge from the AC power line greater than 1 kV the scanner may reset In order to continue normal operation simply cycle power to both the computer and scanner Warning Do not try to gain access to the interior of the instrument Exposure to high voltage electronics can cause severe or fatal injury High voltage electronics can also be found in the scanner s controlling computer See the computer manufacturer documentation on the high voltage hazard warnings Make sure you follow the instructions on the safe operation of the computer Moving Parts The GenePix 4200A Microarray Scanner instrument contains moving parts that can cause injury Under normal operating conditions the instrument is designed to protect you from the moving parts The interlock and protective housing are designed so that you cannot access the moving parts during a scan Warning Do not try to gain access to the interior of the instrument The moving parts inside the instrument can cause injury Fuses There are two 2A 250V fuses located adjacent to the power switch at the rear of the unit Hazardous Material Precautions Use st
39. ios Humanos DHHS siglas en ingl s de acuerdo al Acta de Control de Radiaci n para Salud y Seguridad de 1968 Como la radiaci n emitida dentro del esc ner est completamente limitada a las reas internas y por las cubiertas externas el rayo del l ser no puede escapar durante ninguna fase de la operaci n normal del usuario Advertencia Utilizaci n o ajuste de los controles y la ejecuci n de procedimientos diferentes a los especificados en la gu a del usuario del GenePix 4200A puede resultar en exposici n peligrosa a radiaci n l ser alto voltaje o a partes m viles Exposici n a estos peligros pueden producir lesiones severas o fatales Precauci n Indica que puede ocurrir da o del intrumento p rdida o invalid z de la informaci n obtenida si el usuario no obedece las instrucciones provistas Importante Resalta informaci n fundamental para el funcionamiento ptimo del sistema Nota Identifica aspectos de inter s general Cubierta Protectora La cubierta protectora y el circuito de seguridad del instrumento est n dise ados para protegerlo a usted de exposici n a la radiaci n l ser alto voltaje o a partes m viles Usted no necesita acceder al interior del instrumento En el interior no hay partes que requieran su servicio Advertencia No fuerce el circuito de seguridad ni abra la cubierta protectora o intente acceder al interior del instrumento a trav s de cualquiera de sus aperturas Abrir la cubierta protector
40. irements Universal Input 50 60 Hz 100 240V AC 2A max Space Requirements 25 6 in deep x 16 9 in wide x 13 4 in high 65 cm x 43 cm x 34 cm plus additional 6 in 15 2 cm clearance Weight Requirements Sufficient to support 99 lbs 45 kg with minimal vibration Important Safety Information 42 e Informations de S curit Importantes Informations de S curit Importantes L utilisateur de l instrument laser Microarray Scanner GenePix 4200A est suppos avoir recu la formation ad quate sur l utilisation de cet instrument et les consignes de s curit associ es Le GenePix 4200A Microarray Scanner User s Guide s adresse un op rateur qualifi Informations de s curit utilis es dans ce Guide Veillez prendre les pr cautions indiqu es dans ce guide Rapport de s ret sur le laser Le Centre de Sant pour les Appareils de Radiologie CDRH en anglais de la F d ration Am ricaine des Aliments et des Drogues FDA en anglais a mis en application des r glements concernant les produits au laser fabriqu s depuis le 1 ao t 1976 La conformit est obligatoire pour les produits lanc s sur le march aux Etats Unis Le tomographe est certifi comme produit au laser de la Classe 1 suivant la Norme de Performance de Rayonnement du D partement Am ricain des Services de Sant et Humains DHHS en anglais en accord avec la Loi de 1968 sur le Contr le sur les Rayonnements pour la Sant et la S curit Puisque l
41. lbs con m nima vibraci n Informaci n Importante sobre la Seguridad 52 e Sicherheitsmassnahmen Sicherheitsmassnahmen Es wird vorausgesetzt dass der Benutzer des GenePix 4200A qualifiziert ist das Ger t richtig und sicher zu verwenden Innerhalb der gesamten Beschreibung f r den GenePix 4200A Microarray Scanner bezieht sich das Wort Sie auf diesen qualifizierten Benutzer Sicherheitstext der in dieser Gebrauchanweisung verwendet wird Versichern Sie sich bitte dass Sie alle Vorsichtsmassnahmen beachten Lasersicherheit Aussage Das Center for Devices and Radiological Health CDHR vom U S Food and Drug Administration hat Regelungen f r die Laser Produkte eingef hrt die seit August 1 1976 hergestellt werden Befolgung ist f r die Produkte vorgeschrieben die in den Vereinigten Staaten vermarktet werden Der Scanner wird w hrend ein Class 1 Laser Produkt unter der U S Departments of Health and Human Services DHHS Radiation Performance Standard entsprechend der Radiation Control for Health and Safety Act von 1968 best tigt Innerhalb da die Strahlung die innerhalb des ausgestrahlt wird Scanners vollst ndig sch tzende Geh use und externe Abdeckungen begrenzt wird kann der Laserstrahl nicht w hrend irgendeiner Phase des normalen Teilnehmerbetriebs entgehen Warnung Die Benutzung der Kontrollvorrichtungen nderungen oder das Ausf hren anderer Handlungen ausser denen die in der Gebrauchsanweisung des GenePi
42. ll the SCSI card see below 2 Install GenePix Pro and attach the security key to parallel or USB port For all users 3 Attach the GenePix scanner to the computer with the SCSI cable 4 Attach the power cord to the GenePix scanner 5 Turn on the GenePix scanner 6 Turn on the computer Installing the SCSI Card Included with your GenePix scanner is a SCSI adapter card for your host computer This card is used to transfer data and instructions between the host computer and the GenePix scanner The SCSI card that is included with the GenePix scanner is a PCI based Plug n Play card from AdvanSys that will install easily on most computers We recommend that you use the included SCSI card To install the AdvanSys SCSI card 1 The computer must have an available PCI slot 2 Turn off computer and unplug the power cord Remove the computer cover according to the computer manufacturer s instructions Chapter 2 6 e Installation 3 Remove the AdvanSys SCSI adapter from bag do not touch the metal traces or contacts and carefully insert into the open PCI slot Do not force into place it should go in with a gentle push Screw the endplate to the chassis 4 Replace computer cover plug in power cord and turn on the computer Do not connect GenePix scanner yet 5 Plug and Play operating systems such as Window 2000 and Windows XP will automatically recognize the Advansys SCSI adapter and install the correct driver 6 Install
43. mplifiers Our amplifiers are used around the world by researchers in the neuroscience community We have 20 years of expertise creating low noise amplifiers and high performance computer hardware to digitize these signals Our expertise in this area has been applied to the GenePix scanner Integrated with the GenePix scanner is not only our high performance analog digital converter but also the experience and knowledge to create low noise high performance amplifiers We have employed a unique design to ensure that the PMTs in GenePix scanner are linear over a wide dynamic range and that the signal to noise level exceeds all other currently available microarray scanners General Items of Interest Spatial Resolution The GenePix Professional 4200A is a scanning instrument This means that a single beam of light is rapidly scanned across the microarray and a composite pixilated image is created from the digitized signals from the PMT The spatial resolution of the system refers to the size of the pixel It determines the minimum distance that can be distinguished between two points of light In the GenePix scanner the highest spatial resolution is 5 microns It is possible to change the resolution of Data Scans by changing the pixel size setting in the Hardware Settings dialog of the GenePix Pro software It is important to note that this does not change the size of the scanning beam The GenePix scanner implements changes in resolution by increa
44. nning Eject Ready to scan slide Stage waiting at Slideholder ready to be unloaded the start position ts CAUTION GenePix PROFESSIONAL PRES Axon ici 4200A S Pan EE je js and interlocks defeated CLASS 1 LASER PRODUCT PATENTS PENDING A A DANGER LASER PRODUCT This device complies with LIFTING HAZARD HAZARDOUS VOLTAGE To prevent electric shock and damage to the instrument do not attempt FUSE 250V T2A to open unit 21 CFR 1040 10 amp 1040 11 HEAVY OBJECT POWER FREQUENCY 100 240V 50 60Hz IN WARNING MAX OPERATING CURRENT 1 25 AMP SERIAL NUMBER LABEL NO CUSTOMER SERVICEABLE COMPONENTS ARE CONTAINED WITHIN THIS UNIT CONSULT THE MANUFACTURER FOR REPAIR RETURN INSTRUCTIONS SR THE USER SHALL BE MADE AWARE THAT Axon Instruments Inc IF THE EQUIPMENT IS USED IN ANY MANNER 3280 Whipple Road Union City NOT SPECIFIED BY THE MANUFACTURER California 94587 U S A THE PROTECTION PROVIDED ACIE il eui enc BY THE EQUIPMENT MAY BE IMPAIRED Fax 1 510 675 6300 Site Requirements The GenePix Professional 4200A is designed to operate optimally if the site requirements are met As with any precision optical instrument care should be taken to maintain a low dust low vibration environment Temperature and humidity extremes may compromise performance Environmental Temperature 50 86 10 30 C Environmental Humidity 5 95 non condensing Power Requ
45. nto t cnico a cualquiera de los n meros telef nicos indicados en la etiqueta Lista de Controles Este gu a provee una lista de controles Precauci n el uso de los controles el ajuste o la ejecuci n de procedimientos diferentes de los especificados aqu puede resultar en exposici n peligrosa a la radiaci n del laser Etiquetas de Seguridad Si una etiqueta se hace ilegible o se pierde por algun motivo por favor contacte el servicio t cnico del sistema para obtener etiquetas sin costo alguno Mientras espera por las etiquetas de reemplazo copie las que aparecen en esta secci n y pegue una copia en el instrumento GenePix 4200A User s Guide Copyright 2005 Axon Instruments Molecular Devices Corp Informaci n Importante sobre la Seguridad e 51 Explicaciones de los S mbolos El equipo est a la espera Registrando Scanning Expulse Eject EStandby La platina est lista para descargar La platina est lista en posici n de cargar la diapositiva y empezar Z CAUTION Atenci n Visible laser radiation Peligro al levanter when case is open and interlocks defeated GEASS 1 LASER PRODUCT Br BA Atenci n NDANGER vise HAZARDOUS VOLTAGE Eon To prevent electric shock and damage to the instrument do not attempt to open unit GenePix ProressionaL Axon plain 4200A PATENTS PENDING CLASS 1 LASER PRODUCT This d
46. o DP aede debeas 25 Technical Assistance 27 Warranty and Repair Service 4 eee eee ee eee eee eee eese esee seen e etate se toss et 29 Licensing NotiCE Pr 33 Declaration of Conformity 4 eee eee ee ee ee ee eee eee ee enne seen setate setae etta aee 35 Important Safety Information eee cete ee eee e eerte seta aset an aeta 37 Inc 57 GenePix 4200A User s Guide Copyright 2005 Axon Instruments Molecular Devices Corp Introduction e 1 Chapter 1 Introduction The GenePix Professional 4200A is the latest microarray scanner from Axon Instruments It has several enhanced features for high performance quantitative imaging set by the benchmark GenePix 4000 series scanners Key features of the 42004 are Up to four internally installed laser excitation sources Gradient neutral density wheel permitting 5 100 laser transmission Data Scan resolution from 5 um to 100 um Fast Preview Scan at 40 um Uses 16 bit ultra low noise digitization Dynamic range of 10 Easy access 16 position emission filter wheel with standard filters for each laser installed at the factory Full integration with GenePix Pro and Acuity software Axon Instruments has consulted widely among the scientific pioneers of microarray techniques to produce an inst
47. ources A blue laser with 488 nm excitation can be used with fluorescein and similar dyes and a yellow laser with 594 nm excitation can be used with Texas Red and similar dyes The Alexa Fluor dyes from Molecular Probes offers a number of dyes compatible with all four lasers excitation sources available in the GenePix 4200A scanner Laser excitation sources are often used in scanning systems instead of conventional wide band white light sources such as a xenon or a mercury light source The reasons for this are quite simple conventional wide band light sources do not deliver high enough photon density at the appropriate wavelength for optimum signal to noise ratio In addition it is often difficult to use optical filters to obtain spectrally pure narrow band excitation light Optical Path Excitation laser light is directed onto the slide after passing through a series of filters and mirrors While laser light is by definition very narrow band the GenePix scanner employs additional optical filters to ensure that no GenePix 4200A User s Guide Copyright 2005 Axon Instruments Molecular Devices Corp Imaging System Hardware e 17 spurious excitation light is directed onto the slide If the laser light impinges on an appropriate fluorophore bound on the slide emission light of a longer wavelength is emitted the difference between the emission and excitation wavelengths is called the Stokes shift These emission photons are directed ba
48. p 1040 11 HEAVY OBJECT FUSE E 250V T2A 100 240V 50 60Hz MAX OPERATING CURRENT 1 25 AMP SERIAL NUMBER LABEL MADE IN U S A California 94587 U S A Fax 1 510 675 6300 PATENTS PENDING CLASS 1 LASER PRODUCT CLASS 1 hei DANGER LASER PRODUCT NCAUTION and interlceks defeated GenePix Proressionat Axon Instruments 42 00A E EN hen caso opor HAZARDOUS VOLTAGE To prevent electric shock and damage to the instrument do not attempt to open unit UN warn ns CONSULT THE MANUFACTURER NO CUSTOMER SERVICEABLE COMPONENTS ARE CONTAINED WITHIN THIS UNIT FOR REPAIR RETURN INSTRUCTIONS THE USER SHALL BE MADE AWARE THAT Axon Instruments Inc IF THE EQUIPMENT IS USED IN ANY MANNER 3280 Whipple Road Union City NOT SPECIFIED BY THE MANUFACTURER THE PROTECTION PROVIDED Tel 1 510 675 6200 BY THE EQUIPMENT MAY BE IMPAIRED Conditions n cessaires sur le site Attention danger en soulevant Ms Attention rayonnement laser Attention hautetersion Danger Fe Attention voir les instructions d utilisation Le GenePix 4200A est con u pour fonctionner au mieux de ses performances si les conditions n cessaires sur le site sont satisfaites Comme pour tout autre instrument optique de pr cision il ne doit pas tre expos la poussi re et aux vibrations Des conditions extr mes de temp rature et d humidit peuvent
49. para protegerlo a usted de las partes en movimiento en condiciones normales de funcionamiento El circuito de seguridad y la cubierta protectora impiden el acceso al interior del instrumento durante un escan Advertencia No intente acceder al interior del instrumento Las partes m viles pueden causar lesiones Fusibles Hay dos fusibles 2A 250V adyacentes al interruptor de corriente en la parte posterior de la unidad Precaucions con Materiales Peligrosos El usuario debe utilizar procedimientos y precauciones de laboratorio estandarizados cuando trabaja con productos qu micos Precauci n Utilice siempre las instrucciones de la f brica cuando trabaje con productos qu micos Axon Instruments no son responsables legalmente por da os o lesiones ocasionados como consecuencia del uso de materiales peligrosos Informaci n Importante sobre la Seguridad 50 e Informaci n Importante sobre la Seguridad Paneles Que No Forman Parte del Circuito de Seguridad Vista de las partes superior y posterior del GenePix 4200A mostrando los siete tornillos que aseguran la cubierta protectora NO REMUEVA ESTOS TORNILLOS PELIGRO Radiaci n con laser si se abre EVITE EXPOSICI N DIRECTA AL RAYO LASER Don tremove these screws Mantenimiento y Reparaci n No se requiere ning n mantenimiento por parte del usuario Este no est autorizado para ejecutar ninguna reparaci n Si hay alg n problema por favor comun quese al mantenimie
50. ponents that convert incident photons into electrons via the photoelectric effect When an incident photon impinges on the active surface of the PMT the photocathode an electron is generated This electron flows through a series of electron multipliers dynodes to the Chapter 4 20 e Imaging System Hardware anode The amount of current that flows from the anode is directly proportional to the amount of incident light at the photocathode The amount of amplification that a PMT can produce depends on the number of dynodes in the PMT and the voltage that is applied to the PMT With PMTs it is possible to achieve a gain of 10 When you increase the PMT voltage setting in the GenePix 4200A software you are increasing the gain of the PMTs Note It is important to realize that when you increase the gain of the system you are also increasing the sensitivity to non specific fluorescence thus increasing any electronic noise in the system This increase in noise can often be overcome by line averaging but the cost is decreased acquisition speed In general the signal to noise ratio is not improved by increasing the PMT voltage The PMT in the GenePix scanner meets two essential criteria for high quality imaging First since different photocathodes have varying sensitivities to specific wavelengths of light quantum efficiency the PMT has been carefully chosen for the typical wavelengths of fluorescence emission light produced by microar
51. r les risques li s ces hautes tensions Suivez les instructions concernant les pr cautions d utilisation de l ordinateur Pi ces mobiles L instrument laser Microarray Scanner GenePix 4200A contient des pi ces mobiles pouvant entra ner des risques de dommages corporels Dans des conditions d utilisation normales cet instrument est congu pour vous viter tout risque li aux pi ces mobiles Le syst me de s curit et le bo tier de protection sont con ues pour pr venir tout acc s aux pi ces mobiles durant l utilisation du scanner Avertissement Ne tentez pas d acc der l int rieur de l instrument Il existe des pi ces mobiles l int rieur susceptibles de provoquer des dommages corporels Fusible Il y a deux fusibles 2A 250V situ s c t du commutateur d alimentation l arri re de l appareil Pr cautions contre les substances dangereuses L utilisateur doit suivre les proc dures standard de laboratoires et prendre les pr cautions appropri es la manipulation de produits chimiques Attention Veillez toujours suivre les consignes de s curit du fabricant lors de la manipulation de produits chimiques Axon Instruments ne peuvent tre tenues pour responsables de dommages ventuels directs ou indirects caus s par l utilisation de substances dangereuses GenePix 4200A User s Guide Copyright 2005 Axon Instruments Molecular Devices Corp Informations de S curit Importantes e 45 Acc
52. r similar object can be used to gently maneuver the filter into the seated position do not touch the optical surface as this will irreparably damage the filter but it is safe to touch the black mounting ring Next secure the filter in place with the threaded metallic ring using the filter ring tool It is advisable to record the filter information and position number on a piece of paper Install additional filters if desired and replace filter wheel cassette back into the scanner The access panel must be securely closed before scanning can be resumed To uninstall a filter perform this procedure in reverse order The filter information is stored in the PC board of the scanner not the computer After each filter installation or change is made turn on the scanner and open the Filter Tool program located in the Axon Laboratory GenePix Pro 5 folder of the start menu Activate the filter position by pressing on the check box and enter a description that will help you identify the filter There are two fields available for each position in which you can enter filter information We recommend that you use the filter supplier s designation as the Official name and an easily recognizable description for Alternative name such as the dye name e g FITC Texas Red Photo Detection The GenePix scanner uses a high sensitivity low noise photo multiplier tube PMT to detect the emitted fluorescent light Loosely defined PMTs are optical com
53. rays Second the PMT has been further selected for its reliability and optimal signal to noise performance Mechanical The GenePix scanner is a mechanical device Its laser based scanning system uses a patent pending mechanical design The GenePix scanner operates by slowly scanning the slide in the y direction and rapidly scanning in the x direction The design of the GenePix scanner allows scanning up to four wavelengths of a 25 mm x 75 mm slide at 5 um resolution in about 30 minutes When using such a mechanical device one must be able to monitor the variability in the performance of its mechanical components The GenePix scanner has been designed with a number of optimizations to ensure that variability in the mechanical performance is monitored in real time during acquisition and corrected The GenePix 4200A User s Guide Copyright 2005 Axon Instruments Molecular Devices Corp Imaging System Hardware e 21 utmost attention has been given to selecting and implementing components to create a highly reliable high performance instrument Placing Slides into Slide Holder Standard microscope slides 1 x 3 25 x 75 mm can be used in GenePix scanners Open the door of the scanner and lift up the top of the slide holder by lift on the clasp Hold the slide in one hand with the thumb and index finger positioned near the bottom of the slide Carefully place the slide feature side down into the slide holder and gently click the side sp
54. ring 2 to 3 times to help position the slide into place if necessary Slowly close the top of the slide holder ensuring that the clasp locks into place and close the scanner door Perform a Preview or Data Scan using the controls in the GenePix Pro software Refer to the GenePix Pro user s manual for more information Proper Use of Slide Holder The slide holder retracts into the scanner when the scanner door is closed Opening the door or pressing the Eject button in GenePix Pro will return the slide holder to the loading position It takes a few moments to fully eject the slide holder do not open the slide holder before it has come to a complete stop as this can cause misalignment This type of misuse is evident when scrapes or even paint from the scanner enclosure are found on the slide holder Misalignment of the slide holder caused by prematurely opening the slide holder while the stage is still in motion can result in deterioration of scanner performance and may require servicing Electronic While the optical components of the GenePix scanner are indispensable for generating the electrical current from the PMT the electronic components are just as important They govern the operation of the PMT and the conversion of their analog current signals into a digital signal that can be processed by the host computer Chapter 4 22 e Imaging System Hardware Axon Instruments is the world leader in the design and construction of low noise a
55. rument that fulfills all their image acquisition and primary analysis needs Chapter 1 Installation e 3 Chapter 2 Installation About This User s Guide This User s Guide focuses on the technical and theoretical aspects of the GenePix Professional 4200A hardware We recommend that you use this Guide in conjunction with the GenePix Pro Software User s Guide and the on line Help within GenePix Pro press lt F1 gt For information on setting up your GenePix Professional 4200A scanner and software please refer to Chapter 1 For a guided tutorial on scanning your first slide see Chapter 4 of the GenePix Pro Software User s Guide Computer Requirements Please refer to the Axon Instruments website for up to date information Should you choose to purchase your own computer Axon recommends that due consideration be made for the selection of a quality supplier and or components Chapter 2 4 e Installation Setting Up and Moving the GenePix scanner Placement Place the GenePix Professional 4200A scanner onto a secure laboratory bench top or reinforced table The scanner weighs approximately 45 kg 99 lb and requires two persons to lift Carefully place the scanner into its final position do not slide it into position as this may result in internal damage or misalignment of the optics Moving the Scanner Always use the Park Scanner utility prior to moving the scanner whether making a short move to another location or when
56. ruments Molecular Devices Corp Informaci n Importante sobre la Seguridad e 49 Clasificaci n y Potencia de los L seres Incluidos Longitud de Onda Potencia Divergencia Duraci n TipodeL ser 488 nm 20mW lt 1 2 Continua Clase Ilib 14 4 mW Clase 0 92 mrad Clase llb Clase lb Advertencia Exposici n a la radiaci n l ser puede ocasionar lesiones Por ejemplo mirar directamente a la la radiaci n del l ser puede causar ceguera Peligro de Alto Voltaje El GenePix 4200A Microarray Scanner contiene electr nica de alto voltaje Bajo condiciones normales de operaci n usted est protegido del alto voltaje del instrumento En el acontecimiento improbable que el esc ner reciba una corte de l nea de corriente alterna superior a 1 kV el esc ner se puede reestablecer Para poder continuar la operaci n normal simplemente prenda en ciclos ambas la computadora y el esc ner Advertencia No intente acceder al interior del instrumento Exposici n a alto voltaje puede causar lesiones severas o fatales Electr nica de alto voltaje se encuentra en el computador que controla el esc ner Revise la documentaci n del fabricante sobre las advertencias al respecto Cerciorese de seguir cuidadosamente las instrucciones sobre la utilizaci n apropiada del computador Partes M viles El GenePix 4200A Microarray Scanner contiene partes m viles que pueden causar lesiones El instrumento est dise ado
57. shipping the scanning long distance The Park Scanner utility is accessed by opening the GenePix Utilities program which can be found in the Start Menu GenePix Pro menu or by directly running the GP_Utilities exe executable file located in the GenePix Pro installation directory Instructions 1 Close GenePix Pro if open and start GenePix Utilities program 2 Press Park Scanner button 3 Wait for status message indicating that scanner has been successfully parked 4 Immediately turn off scanner power do not reopen GenePix Pro as this will un park the scanner 5 Place one or two pieces of adhesive tape to secure the door closed Failure to park the scanner may result in misalignment or damage of the optical system Preparing to Install the GenePix Scanner If you have purchased a GenePix scanner without the optional host computer please follow the instructions below If you purchased a GenePix scanner with a bundled host computer the necessary hardware and software are already installed If so skip to Connecting the GenePix Professional 4200A to the Computer GenePix 4200A User s Guide Copyright 2005 Axon Instruments Molecular Devices Corp Installation e 5 Quick Overview of Hardware and Software Installation Here is a brief outline of how to get started with your GenePix scanner Detailed instructions follow immediately below If you have purchased a GenePix scanner without the optional host computer 1 Insta
58. sing the y direction step size and averaging the x direction samples over multiples of 5 um spots For example to change the pixel size from 5 um to 10 um the instrument only scans every second x direction line by increasing each y direction step from 5 to 10 um consequently a 10 um scan is twice as fast as a scan at 5 um and outputs the average of each adjacent pair of 5 um spots in the x direction as the value of a single 10 um pixel Similarly a scan at 20 um pixel size is implemented by scanning every fourth x direction line and so is four times faster than at 5 um and GenePix 4200A User s Guide Copyright 2005 Axon Instruments Molecular Devices Corp Imaging System Hardware e 23 using the average of four contiguous 5 um spots on each x direction line as the value of a 20 um pixel Data Scan pixel size can be increased beyond 20 um in increments of 20 um but the scanning pattern and therefore scan duration remains the same Every fourth x direction line is scanned and the spots within the larger pixel size are averaged For example a 60 um data scan will average the values of thirty six 5 um spots three x direction lines contribute twelve 5 um spots each to calculate the value for each 60 um pixel This is illustrated schematically in the figure below Changing the resolution of a Data Scan may often be useful if you have large features and you do not need to scan at maximum resolution As you decrease the r
59. t of repairing an instrument that has sustained shipping damage It is your responsibility to package the instrument properly before shipping If the packaging is inadequate and the instrument is damaged during shipping the shipper will not honor your claim for compensation Warranty and Repair Service Licensing Notice e 33 Licensing Notice Axon Instruments Molecular Devices Corp is not licensed under any patents owned by Oxford Gene Technology Limited OGT covering oligonucleotide arrays and methods of using them to analyze polynucleotides The purchase of Axon Instruments Molecular Devices products does not convey any license under any of OGT s patent rights including any right to make or use oligonucleotide arrays under OGT s patents Customers may use Axon Instrument Molecular Devices products to analyze oligonucleotide arrays according to OGT s patented methods if those arrays have either been purchased from OGT s licensed suppliers or have been made by the customer under a license from OGT Please contact OGT to enquire about a license under OGT s patents at licensing ogt co uk mailto licensing ogt co uk gt USE OF THIS INSTRUMENT WITH MICROARRAYS MAY REQUIRE A LICENSE FROM ONE OR MORE THIRD PARTIES THAT HAVE PATENTS RELEVANT TO SUCH USE AXON INSTRUMENTS MOLECULAR DEVICES DOES NOT SUGGEST OR PROMOTE THE USE OF THIS INSTRUMENT IN A MANNER THAT INFRINGES ON THE PATENT RIGHTS OF A THIRD PARTY YOU ARE ENCOU
60. uar usando el GenePix 4200A cuando hay s lo un circuito de seguridad funcionando Comun quese al mantenimiento t cnico a cualquiera de los n meros telef nicos indicados en la parte posterior del GenePix 4200A 1 El espejo y el montaje de los lentes siguen movi ndose por debajo de la placa despu s de abrir la puerta 2 La luz azul emitida por el diodo LED con etiqueta Scanning localizado en la parte delantera del GenePix 4200A no se extingue cuando la puerta est abierta 3 Usted no puede registrar ninguna placa 4 Nose puede escuchar el sonido caracter stico del contacto entre metales producido por el circuito de seguridad cuando se ajusta en su lugar Este ocurre normalmente cuando la apertura donde se coloca la placa se abre aproximadamente 6 mm Precauci n Peligro al levantar Advertencia El esc ner GenePix Professional 4200 pesa aproximadamente 45 kg 99 lbs Se requieren dos personas para levantarlo Hay 4 manijas localizadas debajo del esc ner ver la figura Use la t cnica correcta al levantarlo para evitar lesiones CLASS 1 LASER PRODUCT Seguridad del L ser Precauciones No inserte ning n objeto en las aperturas o respiraderos del GenePix 4200A diferente de la placa Puede ocasionar fallas en el circuito de seguridad No intente mover ning n componente debajo de la platina Usted puede causar fallas en el circuito de seguridad GenePix 4200A User s Guide Copyright 2005 Axon Inst
61. upport immediately at either of the numbers indicated on the rear panel of the GenePix 4200A 1 Mirror and lens assembly keeps moving below the slide after the door is opened 2 blue Scanning LED at the front of the GenePix 4200A doesn t extinguish when the door is open 3 You cannot scan any slides 4 You can no longer hear the distinctive metal on metal sound that the mechanical interlock makes when it falls into place This normally happens when the slide loading door has opened about 14 Lifting Hazard Warning The GenePix Professional 4200 scanner weighs approximately 99 lbs 45 kg and requires two persons to lift There are four molded handholds on the underside of the scanner see figure Use appropriate lifting technique to avoid injury CLASS 1 LASER Lasers PRODUCT Cautions Do not stick anything into the openings or vents of the GenePix 4200A other than loading the slide You may cause an interlock to fail Do not attempt to move any parts below the slideholder You may cause an interlock to fail GenePix 4200A User s Guide Copyright 2005 Axon Instruments Molecular Devices Corp Important Safety Information e 39 Embedded Laser Classification and Power Wavelength Power Divergence Duration Embedded Laser s Class Class Illb 144 mW Class 594 nm 1 2 mW 0 92 mrad Continuous Class Illb 635 nm 15 mW 0 7 mrad Continuous Class Illb Warning Exposure to laser radiat
62. urn shipping of the product to the customer If the shipment is to a location outside the United States the customer will be responsible for paying all duties taxes and freight clearance charges if applicable The warranty is valid when the product is used for its intended purpose and does not cover products which have been modified without approval from Axon Instruments Molecular Devices or which have been damaged by abuse accident or connection to incompatible equipment This warranty is in lieu of all other warranties expressed or implied Warranty and Repair Service 30 e Warranty and Repair Service Repair Service The company reserves the right to cease providing repair maintenance parts and technical support for its non consumable hardware products five years after a product is discontinued Technical support for old versions of software products will cease 12 months after they are upgraded or discontinued If you purchased your instrument from a Distributor or OEM Supplier contact them for repair service If you purchased your instrument from Axon Instruments Molecular Devices contact our Technical Support Department If it is determined your instrument must return to the factory for repair the Technical Support Representative will issue a Service Request SR number Our Logistic Coordinator will contact you with specific instructions Out of Warranty Repair Service Out of warranty repair service is available Cont
63. x 4200A erkl rt sind k nnen zu gef hrlichen Strahlungen hoher Voltzahl oder losen Teilen f hren Kontakt mit diesen Gefahren kann zu schweren oder t dlichen Verletzungen f hren Achtung Weist darauf hin dass bei Nichtbeachtung der Vorschriften Schaden am Ger t entstehen kann und Datenausfall oder m glicherweise ung ltige Daten resultieren k nnen Wichtig Weist auf Information hin die wichtig ist um die optimale Leistung des Ger tes sicherzustellen Anmerkung Deutet auf Themen von generellem Interesse hin Das Schutzgeh use Das Schutzgeh use und die elektrische Verriegelung sind so konstruiert dass Sie vor Laserstrahlen Hochspannung und beweglichen Teilen gesch tzt sind Sie brauchen das innere Teil des Ger tes nicht zu ffnen weil keine f r den Nutzer justierbaren Teile vorhanden sind Warnung Ver ndern Sie nicht die elektrische Verriegelung ffnen Sie nicht das Schutzgeh use oder versuchen Sie gleichermassen nicht das Innere des Ger tes durch andere Offnungen zu erreichen Der GenePix 4200A ist ein Laserprodukt der Klasse 1 Auf den Bereich der im Ger t entstehenden st rkeren Laserstrahlen hat der Benutzer kein Zugriff Die bewegliche Spiegel und Linsenmontage die sich unter dem Objekttr ger befindet lenkt und fokussiert diese Laserstrahlen der Klasse 3B direct nach oben auf die Oberfl che des Tr gers mit dem Pr parat Glasstreifen k nnen sicher in den Laserrasterbereich eingef hrt werden weil die la

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