Instrument catalog
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1. r e ue ur aa r r e r a i q i ml Channels ay 1 8 Be i I pa Waste 1 Solvents P Waste 6 T ee ES T i E f zj es lea Tica a Channels Hl 1 8 i l l l l l i e e e e eS e e e e l As shown in the above diagram 3 columns are used for each sample Samples are first loaded to column C1 The effluent from column C1 can be diverted to waste to column C2 or column C3 This setup provides capability for very complex samples PromoChrom Instruments SPE 04 www promochrom com 9 4 Specifications Sample capacity Volume of sample Material of wetted parts System control Method Method functions Pump flow rate Pressure limit of pump Pump reproducibility C V Temperature of evaporation Power supply Current Weight Dimension cm 5 Ordering Information Part Number SPE 03 01 4 6 or 8 per batch 1 to 4000 mL Teflon PEEK Pyrex glass Micro controller with LCD and keypad Permanent storage of three methods Pre condition load sample elution with 5 solvents blow dry of sorbent fraction collection 1 to 85 mL min 6 bar lt 1 5 Ambient to 90 degrees 24 VDC lt lA 13 Kg 34 x 34 x 43cm width x depth x height Description Price US Includes 4 channel SPE 03 mainframe 24V power supply and user manual SPE 03 02 Includes 8 channel SPE 03 mainframe 24V power suppl2. PromoChrom Instruments SPE 04 www promochrom com 11 Depending on the configurations a SPE 04 can perform offline solid phase extraction online solid phase extraction online derivatization and direct injection to HPLC The online derivatization function is very useful for analysis of amino acids hormones and some pesticides 2 2 Easy operation SPE 04 PromoChrom SEE SPE 04 uses built in methods to do offline SPE It does not need a computer The operation of instrument involves only 7 buttons The operation elute C1 with 1 procedures of the offline SPE is the same as SPE 01 gis che and SPE 03 collect fraction blow air load fraction to heater default txt steprun tst test txt The screen indicates samples 5 to 18 are to be processed using method 2 It is now processing sample 7 The total volume per sample is 20 5 mL Currently the instrument is performing add sample action In online mode the collected fraction is directly injected into an HPLC or LC MS for final determination The control software for online SPE is user friendly and is compatible with most HPLC software The software uses methods and sequences for the automation It has similar structure as Agilent Chemstation Users of HPLC can easily understand the SPE 04 software The software can perform overlapped injection When HPLC is performing an HPLC run SPE 04 can start processing the next sample PromoChrom Instruments
3. SPE O1 I use a stream selection valve and a syringe pump to transfer elution solvents or o sample liquid to the SPE columns Although all the samples share the same syringe pump and the valve cross contamination is avoided as the flow path in the valve and the syringe pump are continuously washed during the elution procedures Besides the instrument has built in clean procedures to wash the tubing for samples and the flow path automatically to make sure all the sample contacting surface is fully cleaned The flow path in the valve is specially designed so that the sample liquid has minimum contact with the stator surface when the valve is turning This feature is very helpful for avoiding contamination of sticky compounds such as benzopyrene on the surface of the stator www promochrom com 1 PromoChrom Technologies SPE 01 flow path in a Conventional Flow path for the valve used in SPE 01 selection valve As shown in the diagram below the major components of SPE 01 IT are a stream selection valve and a channel distribution module Each channel is arranged closely and each sample has isolated flow path removing possibilities of cross contamination N2 tor drying of columns Solvent sele ction valve Channel distribution module Solvent 1 6 Channel 1 8 l de I i ee ee 2 Features 2 1 Easy operation SPE 01 uses built in methods They can be easily edited
4. SPE 04 www promochrom com 12 3 Applications Direct analysis of hormone in plasma sample 1 Dilute plasma sample with 1 phosphoric acid at 1 5 ratio 2 Precondition a 3 mL 200 mg C18 SPE column with 2 mL methanol followed by 2 mL water 3 Load 2 mL sample and wash with 4 mL water methanol 80 20 4 Wash SPE column using methanol and collect 1 mL fraction 5 Derivatize the fraction with dansyl chloride at 60 C 6 HPLC analysis using a PCTsil C18 column and UV or fluorescence detection dos OCA 52 ot a a r a ag ae L hs EA e ma a a y Si N Estrone Dansyl chloride eo CHS PromoChrom Instruments SPE 04 www promochrom com 13 4 Specifications Sample capacity Maximum sample volume Material of wetted parts System control Method functions Temperature for derivatization Type of derivatization reagent Pump flow rate Pressure limit of pump Pump reproducibility C V Power supply Current Weight Dimension cm 5 Order information Part No SPE 04 01 Offline model 18 26 or 38 per batch 4 8 or 20 mL Teflon stainless steel Pyrex glass Micro controller with LCD and keypad Pre condition load sample elution with 5 solvents fraction collection 1 to 30 mL min 6 bar lt 1 5 24 VDC lt lA 12 Kg 34 x 42 x 35 cm width x depth x height Description power supply and user manual Online model 18 26 or 38 per batch 4 8 or 20 mL Tefl
5. of a silica particle NH Aminopropyl SPE Cartridge Description Tubes Box Part Number Price US Si O Si CH NH 100mg 1 mL 100 NH 010 1 108 O j 200 mg 3 mL 50 NH 020 3 75 gj OH 500 mg 3 mL 50 NH 050 3 88 d 500 mg 6mL 30 NH 050 6 58 Structure of amino NH3 silane covalently bonded 1000 mg 6 mL 30 NH 100 6 85 to the surface of a silica particle PSA N aminoethyl Aminopropyl SPE Cartridge Description Tubes Box Part Number Price US gi Si CH NH CH NH 100 mg 1 mL 100 PA 010 1 108 a 7 o ae ee 200 mg 3 mL 50 PA 020 3 85 gi OH 500 mg 3 mL 50 PA 050 3 88 a 500 mg 6 mL 30 PA 050 6 38 Structure of PSA silane covalently bonded to the 1000 mg 6mL 30 PA 100 6 85 surface of a silica particle www promochrom com 25 PromoChrom SPE Cartridges Silica Based Sorbents SAX Strong Anion Exchanger SPE Cartridge Description Tubes Box Part Number Price US one Pee i a a z 100 mg 1 mL 100 SA 010 1 108 vin E i CH N CHs Cl 200 mg 3mL 50 SA 020 3 88 gj OH 500 mg 3 mL 50 SA 050 3 108 500 mg _6 mL 30 SA 050 6 78 Structure of SAX silane covalently bonded to the 1000 mg 6 mL 30 SA 100 6 118 surface of a silica particle COOH Weak Cation Exchanger SPE Cartridge Description Tubes Box Part Number Price US eae OE a Si O Si C OO 100mg 1 mL 100 CH 010 1 108 Ti B a Loca 200 mg 3 mL 50 CH 020 3 88 OH 500 mg 3 mL 50 CH 050 3 108 a _ Omg Oml 300 Structure of pro
6. The operation of instrument involves only 7 buttons Below is a typical routine operation procedure e Place sample tubes and add samples e Install columns and collection tray e Select method e Press the start stop button The screen indicates samples 1 to 4 are being processed using The instrument will process the samples method The total volume per sample is 150 0 mL and 140 automatically according to the selected mL has been processed Currently the instrument is running the step of elution using solvent 2 method PromoChrom Technologies SPE 01 www promochrom com 2 2 2 Column blockage detection and smart handling The system can detect the blockage of SPE column and reduce the flow rate accordingly If blockage still occur the instrument will pause to wait for human attendance 2 3 Small footprint and simple structure Normal automated SPE instruments involves many switching vales and complex tubing connections A multi channel SPE is normally heavy and of large size The tubing connection is also complex making maintenance difficult Thanks to the flow path integration technique the weight and dimension of SPE 01 are around half of other multi channel SPE instruments The weight of a SPE O1 II is only 12 kg 2 4 Adaption of various SPE columns az af m l SPE 01 adopts an innovative general purpose adapter to deal with variations in diameter of SPE columns The adapters can be easily adjusted whe
7. aromatic hydrocarbon PromoChrom Instruments SPE 01 www promochrom com 5 4 Specifications SPE 01 1 SPE 01 II Sample capacity 8 per batch one by one mode 8 per batch parallel mode Volume of sample 1 to 50 mL 1 to 50 mL Material of wetted Teflon 316 stainless steel Pyrex Teflon 316 stainless steel Pyrex parts glass glass Microcontroller with keypad data Microcontroller with keypad data System control entry entry Permanent storage of three methods Permanent storage of three methods Method ad he with instrument with instrument Pre condition load sample elution with 5 solvents wash of sample line drying with nitrogen fraction collection Pump flow rate 0 5 to 85 mL min 0 5 to 85 mL min Pressure limit of pump 6 bar 6 bar Pump reproducibility C V lt 1 5 lt 1 5 Power consumption lt 1 0 A at 24 VDC lt 1 5 A at24V DC Weight Kg 11 5 12 5 34 x 34 x 45 width x depth x 34 x 34 x 45 width x depth x height height Pre condition load sample elution Method functions with 5 solvents wash of sample line fraction collection Dimension cm 5 Order information Part No Description Price US SPE 01 01 Includes SPE O1 I mainframe 24V power supply and user manual Includes SPE O1 II mainframe 24V power supply and user manual SPE 01 02 PromoChrom Technologies SPE 03 www promochrom com 6 SPE 03 Multi Channel Cleanup Station SPE 03 is the most versatile and f
8. to install software for most applications This feature makes 1t easy to move from one LC to another without disturbing the analytical system 2 3 Overlapped sample preparation When HPLC or LC MS is analyzing a sample LC 03 will start processing the next sample It helps to save time in sample preparation 2 4 Easy integration with HPLC The instrument provides a remote port to trigger start stop of HPLC It can be used with any HPLC or LC MS that has a remote input port 3 Applications Below is an example for direct analysis of polycyclic aromatic hydrocarbons PAH in tap water at ppt level A 40 mL tap water is analyzed directly Since the whole portion of the sample is injected to the analytical column the detection limit can go down to 3 ppt without using fluorescence detection By using the overlapped injection feature the processing time for one sample is only 15 minutes In an offline approach normally 500 to 1000 mL water need to be extracted Further concentration and solvent exchange are followed before the instrument analysis Only a very small portion of the concentrated sample is injected to the HPLC To achieve similar detection limit of online SPE 4 liters of water need to be extracted The processing time for one sample is above 1 hour Another advantage of online SPE over offline SPE is its good reproducibility Since online SPE involves fewer procedures than the offline approach and all these procedures are controlle
9. 16 5 x 22x 26 cm width x depth x height 5 Ordering Information Part Number Description Price US Online SPE Include hardware control software USB RS232 converter and 24V power supply Method kit for compounds of low polarity Include 5 TrapN SPE column one analytical column and methods LC 03 03 Method kit for compounds of high polarity Include 5 TrapP Eee us SPE column one analytical column and methods www promochrom com 18 PromoChrom Instruments SPE 06 SPE 06 Mini SPE SPE 06 miniSPE is a flexible and versatile device for automated solid phase extraction In spite of its small size and much lower cost than other automated SPE instruments SPE 06 can perform all actions of SPE such as column conditioning sample loading elution and fraction collection SPE 06 has two configurations Configuration 1 can handle up to 4 samples Configuration 2 can handle 2 samples and can make elution of SPE columns in two directions 1 Features 1 1 Easy operation SPE 06 uses built in methods They can be easily edited The operation of instrument involves only 7 buttons Below is a typical routine operation procedure e Place sample tubes and add samples e Install columns and collection vials e Select method e Press the start stop button The instrument will process the samples automatically according to the selected method When the device is processing samples its status is closely monitored an
10. 190 Price US 210 Price US 210 Price US 90 750 160 Graphitized Carbon Black Average particle size 120 400 mesh Specially for sample cleanup in pesticide residue analysis Alternative to Supelco Envicarb Two layer sorbents isolated with a frit Effectively remove pigments and organic acids from plant samples Widely used for sample cleanup in pesticide residue analysis Alternative to Supelco Envi Carb NH2 Two layer sorbents isolated with a frit Effectively remove pigments and organic acids from plant samples Widely used for sample cleanup in pesticide residue analysis Alternative to Supelco Envi Carb PSA Pesticarb Graphitized Carbon Black Average particle size 120 400 mesh Silica bonded with PSA Silica 40 60 um bonded with primary secondary amine www promochrom com 27 Terms and Conditions Price Prices in this catalog are for reference only and may change without notice The prices do not include tax and costs on shipping and handling Please ask PromoChrom or an authorized distributor for a quotation before placing your order Design Changes Due to continuing improvements in design some items may differ slightly from the descriptions and photographs Specifications are subject to change without notice Warranties The warranty period for instruments is one year If notice of defects is received within the warranty period ProomoChrom shall at its option either repair or rep
11. PE 03 is as follows Step Action oe ae sra Remarks 0 Elute 1 10 10 0 Condition column with 10 mL methanol Elute 2 10 10 0 Condition column with 10 mL water Add samp 10 750 0 Load 750 mL sample Elute 2 10 10 0 Wash SPE column with water Elute 3 10 0 3 Change elution solvent to ethyl acetate 1 2 3 4 Blow air 20 40 Purge away water from the SPE column 5 6 Collect 10 10 Elute with ethyl acetate and collect a 10 mL fraction PromoChrom Instruments SPE 03 www promochrom com 8 The volume of collected fraction was reduced to 1 mL and analyzed using a GC MS system The procedures extraction using a vacuum manifold are similar to the SPE 03 method The flow rated is controlled in similar range or slower by adjusting the vacuum Recovery test results of 8 triazole pesticides in tap water Recovery Standard Deviation n 3 Pesticides Manual Automated Manual Automated Triadimefon 76 99 14 6 Paclobutrazol 83 97 14 6 Hexaconazole 88 100 14 5 Uniconazole 83 93 13 5 Flusilazole 90 93 5 6 Tebuconazole 82 93 6 4 Due to a well controlled flow rate SPE 03 gives a much better recovery and reproducibility 3 2 Cleanup for pesticide residues using multiple columns SPE 03 provides a powerful platform for sample cleanup in pesticide residue analysis In addition to all the capabilities of the SPE 01 SPE 03 can use multiple columns for multi residue cleanup Solvent selection valve Channel distribution module
12. ast sample preparation system It can process up to 8 samples simultaneously The sample volume ranges from 0 1 mL to 4000 mL SPE 03 also provide synchronized evaporation during the fraction collection Thanks to the flow path integration technique SPE 03 has a simple structure reliable performance and more reasonable price 1 Working principle of SPE 03 The following diagram illustrates the working principle of SPE 03 Ai mlet Solvent sele ction valve Solvents __ Brian A aa Se Organic waste Aqueous N2 line waste N2 for drying OS Channel distribution module As shown in the above diagram the functions of SPE 03 are integrated into 3 4 modules It does not need other stream selection valves or 3 way solenoid valves The tubing layout is also very simple 2 Features 2 1 Easy operation SPE 03 uses built in methods for automatic extraction The methods can be easily edited and saved for repeated use The operation of instrument involves only 7 buttons Below are typical routine operation procedures e Place sample inlet probes in samples e Place columns and receiving containers e Select method e Press start stop button PromoChrom Technologies SPE 03 www promochrom com 7 2 2 Blockage detection and smart handling The system can detect the blockage and reduce the flow rate accordingly If blockage still occurs at the minimum flow rate the instrument will pause and wait for hu
13. atrix The strength of elution solvents can then be increased stepwise In handling multi residue analysis pesticides of different polarity may be collected into two fractions Existing methods can be easily modified and used for SPE 01 plus automation process Since it is hard to control the flow rate and volume of solvents the repeatability of manual SPE is not satisfactory and is mainly used for simple clean up SPE 01 can control flow rate accurately and helps to improve reproducibility of the analysis With SPE 01 users can do multi step elution using up to 5 solvents Therefore the instrument is very useful for the clean up of complex samples and especially for the simultaneous analysis of multi residues 3 2 Accelerated elution Flash column chromatography The flow rate in manual column cleanup is limited by gravity and particle size of the sorbent In addition to unstable flow rate the elution cannot be accelerated In organic synthesis field column chromatographic purification has seen a drastic improvement after introduction of flash HPLC which uses higher flow rate and sorbent of smaller particles However this technique is seldom used for sample cleanup in trace analysis due to the lack of suitable automated cleanup instruments The performance of the pump in SPE 01 is comparable to the pump used in a flash LC It can deliver a flow rate up to 50 mL min The high output pressure also allows to use smaller particles 10 20 um to imp
14. ca gel column and determination with GC FID The column cleanup is complex and time consuming due to multiple elution steps large elution volume and multiple fraction collections Since the cleanup uses a very large column 5 gram 25 mL and the process volume is also large each sample needs two fractions with volume up to 50 mL most automated SPE on the market cannot meet the needs SPE 01 provides a perfect solution for this application As an added advantage SPE 01 s design can tolerate the small particles from the sample matrix and aggressive solvents used for the elution Below is the sample preparation procedures Extract 10 Concentrate Cleanup using Concentrate Analysis with gram soil using the extract and 5 gram 25 mL two fractions methylene change solvent silica column to 1 mL chloride to hexane Corresponding method for SPE 01 Solvent 1 Hexane solvent 2 methylene chloride Flow rate Volume Step Action fina mL Remarks 0 Elute 1 10 10 0 Condition column with 10 mL hexane Blow air 10 5 0 Blow dry sorbent to avoid sample dispersion Add samp 5 5 0 Exhaustic loading of 1 mL sample in the sample vial 1 2 3 Elute 1 10 1 0 Change elution solvent to hexane 4 Collect 1 5 15 Continue elute with hexane and collect 15 mL fraction contains aliphatic hydrocarbon Elute 2 10 0 5 Change elution solvent to methylene chloride Collect 2 10 30 Continue elute with methylene chloride and collect 30 mL fraction contains
15. d by instrument the chance of error is much less Even at 0 05 ppb level the RSD is only 2 5 n 3 Therefore if the pollutant can be analyzed by HPLC the online approach should be used The key for a successful application of online SPE is the selection of a suitable SPE column and the analytical column To simplify the method development PromoChrom provides two method kits with www promochrom com 16 PromoChrom Instruments Online SPE for water analysis well matched SPE column and the analytical column They also include the method parameters for most common analysis By slightly adjusting the parameters users can easily development their own methods O VWWD1 A Wavelength 252 nm ONLINE SPE DATA PHENATHRENEOO22 D a Phenanthrene 0 05 ppb inject 40 mL 0 1 2 O VWWD1 A Wavelength 252 nm ONLINE SPE DATA PHENATHRENEOO29 D 250 Phenanthrene 5 ppb inject 40 mL 0 1 2 3 4 5 6 z mi Direct analysis of phenanthrene in tap water at 5 ppb and 50 ppt level using online SPE coupled with HPLC SPE column TrapN analytical column PromSil C18 sample volume 40 mL flow rate for sample loading 6 mL min detection wavelength 252 nm www promochrom com 17 PromoChrom Instruments Online SPE for water analysis 4 Specifications Pressure limit of pump 10 bar Material of wetted parts Stainless steel Teflon PEEK special glass System control Software via computer Power supply 24 VDC Weight 4 0 Kg Dimension
16. d indicated As shown in the screen on the right it indicates taht sample 3 is being processed using method 1 The total volume per sample is 2100 5 mL and 150 mL has been processed Currently the instrument is running the step of elution using solvent 3 The built in methods use very simple commands and can be easily modified Below is an example of a method 3 Addsamp 6 200 Load 20 mL sample at 6 mL min flow rate 4 Rinse 2 10 Use 1 mL solvent 2 to rinse sample tube and add rinsate to column 5 Ewe3 6 00 Change elution solvent to solvent 3 fraction www promochrom com 19 PromoChrom Instruments SPE 06 1 2 Column blockage detection and smart handling The device can detect the blockage of SPE columns and reduce the flow rate accordingly If blockage still occur the instrument will pause to wait for human attendance 1 3 Small footprint and simple structure due to flow path integration technique Thanks to the flow path integration technique SPE 06 has a very simple and a very small size 16 5 x 22x 26 cm Its weight is below 3 Kg It can be easily carried and may be used for on site sampling 1 4 Use of various SPE columns and containers SPE 06 uses an innovative general purpose adapter to deal with variations in diameter of SPE columns These adapters can be easily adjusted when different type columns are used It is not necessary to use extra caps or adapters for different columns The dev
17. ice can use various types of containers for sample and fractions such as Erlenmayer flasks beakers and test tubes The containers can be capped to prevent solvent evaporation www promochrom com 20 PromoChrom Instruments SPE 06 2 Specifications Sample capacity 4 samples per batch sequential process Sample volume 1 2000 mL Number of fraction 1 fraction per sample Type of elution solvent Solvent wetted material 316 stainless steel Teflon and Pyrex glass Control Micro controller control with keypad input and LCD display Weight 2 5 Kg Dimension 16 5 x 22x 26 cm W x Dx H Order information Product ber Description Price US Include SPE 01 mainframe 24 V power supply and user manual SPE 06 01 www promochrom com 21 PromoChrom Instruments LC 07 Syringe pump LC 07 multi channel syringe pump LC 07 consists of a syringe pump and a stream selection valve It can deliver liquids from one location to another accurately and automatically With the built in program LC 07 can be used to make a set of standard solutions or deliver chemical reagents to a chemical reactor according to pre set time Its operation involves only 7 buttons Like other instruments from PromoChrom the operation of LC 07 is simple and very user friendly Users can command the use of the device quickly by referring to the user manual A routine operation procedures only involves 3 steps 1 place the containers 2 Select
18. lace the defective products If PromoChrom is unable to repair or replace the defective products within a reasonable time buyers shall receive refund of the purchase price upon return of the products The warranty for defects is limited to the purchase price of the product In no event shall PromoChrom Technologies be liable for incidental or consequential damages in connection with the furnishing performance or use of the products PromoChrom Technologies Ltd 212 10951 Mortfield Road Richmond BC V7A 2W5 Canada Tel 1 604 2950280 Fax 1 778 2975517 Email info promochrom com Websites www promochrom com 5 bi PromoChrom Technologies
19. man attendance 2 3 Small footprint and computer free operation The instrument has a very small footprint and does not need a computer For a 8 channel SPE 03 the weight is below 13 Kg The elution solvents are placed on the top of the instrument The compact design helps to save precious laboratory space 2 4 Accommodate various columns and sample fraction containers SPE 03 uses a general purpose adapter to fit columns of various size ranging from 1 to 24 mL It is not necessary to use other caps or adapters Various containers such as Erlenmeyer flasks flasks for rotary evaporators and test tubes can be used for samples and fractions It helps to reduce liquid transfer for the following sample preparation procedures The containers can all be capped to prevent solvent evaporation 2 5 Integrated online evaporation SPE 03 has a built in evaporation module as an option It can perform evaporation while the instrument 1s doing fraction collection By controlling the speed of elution an evaporation a large volume fraction can be collected into a 1 5 mL auto sampler vials directly 3 Applications 3 1 Extraction of pollutants in large volume water samples A recovery test of 8 triazole pesticides in water was carried using both conventional manifold extraction and a SPE 03 automated SPE A volume of 750 mL tap water was spiked with the 8 pesticides at 1 ppb level and extracted using 500 mg 6 mL PCTsil C18 cartridges The methods of S
20. mension cm 16 5 x 22x 26 3 Order information Part number Description Reference price US Include a LC 07 mainframe with 10 port valve LC 07 01 24 volt power supply and user manual Include a LC 07 mainframe with 18 port valve 24 volt power supply and user manual LC 07 02 www promochrom com 24 PromoChrom SPE Cartridges Silica Based Sorbents PromoChrom Silica Based Sorbents C18 C8 NH2 PSA SAX SCX COOH Made of high quality spherical and pure silica particles Average particle diameter 45 um Sorbent surface is specially modified to ensure high Average Pore Size 60 A sample recovery and good reproducibility Pore Volume 0 8 cm g Specific Surface Area 480 m7 g C18 Endcapped SPE Cartridge 7 Description Tubes Box Part Number Price US Si O Si CH CH 100 mg 1mL 100 18 010 1 C 108 O vee 020 3 an ee 200 mg 3 mL 50 18 020 3 C 75 Si O Si CH 500 mg 3 mL 50 18 050 3 C 88 500 mg 6 mL 30 18 050 6 C 68 Structure of C18 silane and trimethyl silyl 1000 mg 6 mL 30 18 100 6 C 85 endcapping group covalently bonded to the surface of a silica particle C8 SPE Cartridge Description Tubes Box Part Number Price US ve 51 0 51 CH CH 100 mg 1 mL 100 08 010 1 108 a ee ae 2 L z 2 z k ta E x 500 mg 3 mL 50 08 050 3 88 500 mg 6mL 30 08 050 6 68 Structure of C8 octyl silane and trimethyl silyl 1000 mg 6mL 30 08 100 6 85 endcapping group covalently bonded to the surface
21. n different type columns are used 2 5 Automatic wash of sample tubing To prevent cross contamination function for cleaning of sample tubing is built into the methods After loading sample then tubing will be washed automatically using solvent It is followed by air purge to remove leftover solvents As the wash direction is opposite to the loading direction the small particles accumulated on sample filters are effectively removed As this function is in the methods it is not necessary to wash the tubing separately after the extraction 2 6 Easy transfer of existing manual methods Below is an example of methods for SPE 01 Action Elute 2 Elute 1 Addsamp 6 20 Elute 2 Collect 1 Elute 3 Collect 2 The format is very similar to manual methods Any manual procedure for column cleanup can be conveniently transferred to an instrument method PromoChrom Technologies SPE 01 www promochrom com 3 3 Applications 3 1 Column cleanup for analysis of drug and pesticide residues in food samples Traditional column cleanup uses glass columns packed with silica gel alumina or Florisil Now pre packed solid phase extraction cartridges are getting popular A typical clean up method involves pre conditioning of column using a strong solvent such as acetone followed by a weak solvent such as hexane After adding the sample columns are first eluted with a weak solvent to remove lipids and other low polarity components from sample m
22. oad inject sample add reagent 1 inject start LC etc The device uses remote signal to trigger start of HPLC While the HPLC is running a sample online SPE can carry out extraction of next sample The overlapped introduction saves the time for sample preparation Below is a typical working cycle 1 Conditioning of the extraction column The stream selection valve is diverted to an elution solvent the syringe pump transfers the solvent to the extraction column 2 Load sample The stream selection valve is diverted to a sample container The syringe pump add the sample to the extraction column 3 Elution the extraction column to remove interference The stream selection valve is connected to an solvent again and the syringe pump uses the solvent to rinse the extraction column 4 Inject sample to HPLC The 2 position injection valve is switched from load position to inject position 5 The SPE system send a trigger signal to HPLC The mobile phase from HPLC carries the sample from the extraction column to the analytical column and start the HPLC analysis www promochrom com 15 PromoChrom Instruments Online SPE for water analysis 2 Features 2 1 Easy operation The online SPE system has a very user friendly software The diagram displays the liquid path in a real time manner The command is straight forward 2 2 Stand alone operation The online SPE can be cantroled using keypad it is not necessary
23. on stainless steel Pyrex glass Computer or micro controller with LCD and keypad Pre condition load sample elution with 5 solvents blow dry of sorbent fraction collection injection into HPLC Ambient to 80 C 2 1 to 30 mL min 6 bar lt 1 5 24 VDC lt lA 12 Kg 34 x 42 x 35 cm width x depth x height Includes SPE 04 offline mainframe no online SPE function 24V Includes SPE 04 mainframe sample injection module for HPLC SPE 04 02 user manual control software remote cable for HPLC 24V power supply and Includes SPE 04 mainframe sample injection function for HPLC online derivatization module and control software remote cable for HPLC 24V power supply and user manual SPE 04 03 www promochrom com 14 PromoChrom Instruments Online SPE for water analysis LC 03 Online SPE for Direct Analysis of Liquid Samples Online SPE is for direct analysis of water samples It combines sample extraction and LC or LC MS analysis Part from onlme SPE ee Online SPE PromoChrom SEE 1 Working principle Initialize Configure Report Method Sequence Steprun Diagnosis i in i The online SPE uses valves and a syringe pump to transfer sample to SPE column and then to the HPLC system The control software looks after the elute with 2 elute with 1 detailed valve switches Users only need to flush sample Ine 0 5 load sample elute with 1 select the necessary actions Such as l
24. p y PromoChrom Technologies Instrument Catalog 2013 2014 Table of Contents SREHO PCleanup SAUD is l SPE 03 multi Channel Cleanup Station ccc cece eens 7 SPE 04 Multi functional Cleanup Station 4 1 Le 05 Onmeda 15 SE ESDO MINT SPEAR 19 ECO Syringe PUND rearrerioracrp aran ENSEN 22 SPE CaA Td ES lodo 25 Terms and Condition a ds aeee 28 PromoChrom Technologies SPE 01 SPE 01 Cleanup Station SPE 01 solid phase extraction system is designed for automatic sample cleanup in analysis of food and yl ray A environmental samples It can be used for pesticide residue and drug residue analysis in food samples and for analysis of pollutants in soil samples SPE 01 can automatically fulfill all the necessary actions for solid phase extraction and column chromatographic cleanup such as conditioning of columns sample loading washing and fraction collection By providing constant flow rate and well controlled elution procedures SPE 01 helps to improve quality and efficiency of trace analysis and release chemists from tedious sample preparation routines PEPE SPE 01 provides two configurations to fit the sample throughput a Wg LER needs SPE 01 I handles 8 samples one by one single channel pos design whereas SPE O1 II processes 8 samples in a parallel mode 8 channel design 1 Working principle of SPE 01 The following diagram illustrates the working principle of SPE 01 I
25. pyl carboxylic acid COOH silane 1000 mg 6 mL 30 CH 100 6 118 covalently bonded to the surface of a silica particle SCX Strong Cation Exchanger SPE Cartridge Description Tubes Box Part Number Price US a eee e E 1 ER S E sal h O 100 mg 1mL 100 SC 010 1 108 Va nse 200 mg 3 mL 50 SC 020 3 88 o_O 500 mg 3 mL 50 SC 050 3 108 ts 500 mg 6 mL 30 SC 050 6 78 Structure of SCX silane covalently bonded to the 1000 mg 6 mL 30 SC 100 6 118 surface of a silica particle Silica SPE Cartridge Description Tubes Box Part Number Price US Noi OH 100 mg 1 mL 100 SI 010 1 90 a 200 mg 3 mL 50 SI 020 3 68 oi OH 500 mg 3 mL 50 SI 050 3 78 7 500 mg 6 mL 30 S1 050 6 54 Structure of silanol groups on the surface of a silica 1000 mg 6 mL 30 SI 100 6 70 particle www promochrom com 26 PromoChrom SPE Cartridges for pesticide residue analysis PromoChrom Sorbents for pesticide residue analysis PestiCarb SPE Cartridge Description Tubes Box Part Number 200 mg 3 mL 50 PC 020 3 500 mg 3 mL 50 PC 050 3 500 mg 6 mL 30 PC 050 6 1000 mg 6 mL 30 PC 100 6 PestiCarb NH2 Description Tubes Box Part Number 500mg 500mg 6 mL 30 PCNH 050 6 PestiCarb PSA SPE Cartridge Description Tubes Box Part Number 500mg 500mg 6 mL 30 PCPA 050 6 Sorbent for dispersive SPE QuEChers Quantity Description g Part Number Pesticarb 10 SBPC 010 Pesticarb 100 SBPC 100 Silica bonded with PSA 100 SBPA 100 Price US 120 168 130
26. rove the column efficiency Below is an example of its application for pesticide residue cleanup in tea Flow rate 6 ml min Flow rate 18 mL min Elution pattern of 5 pyrethroid insecticides on column packed with 3 gram florisil fenpropathrin cyhalothrin cypermethrin fenvalerate deltamethrin PromoChrom Technologies SPE 01 www promochrom com 4 As shown in the above figures elution pattern at 18 mL min is similar to that at 6 mL min near the flow rate in manual column cleanup A much faster elution speed can be achieved in automated column cleanup Currently the particle size of packing material for SPE cartridges and column chromatographic cleanup is normally of 40 um and above Although it is known that a smaller particle gives better separation efficiency and reduces elution volume in chromatography it is not practical in manual operation as the liquid will have problem flowing out With the introduction of SPE 01 it becomes possible to use columns with smaller particles 3 3 Cleanup for analysis of extractable petroleum hydrocarbons EPH in soil The New Jersey Department of Environmental Protection NJDEP released a method for analysis of extractable petroleum hydrocarbons EPH It can be used for separate quantification of aliphatic and aromatic hydroncarbons in environmental samples water soil sediment and sludge The method involves extraction of sample using methylene chloride cleanup using sili
27. the method 3 Press the Start button The device will complete the work automatically LC 07 uses built in methods to achieve automatic liquid delivery The meaning of the method is straight forward refer to the above picture Each step in a method can set timing intake port output port pump flow rate and volume Up to 16 steps can be set in a method The device use most inert material for solvent contact parts Teflon 316 stainless steel and Pyrex glass They are compatible to most aggressive solvents 1 Applications 1 1 Automatic preparation of standard solutions Making standard solutions is a routine work for an analytical laboratory Its accuracy decides the quality of the analytical data It is time consuming and need skillful personnel A LC 07 can fulfill the tasks automatically The following example demonstrates how to make a series of 5 standard solutions from a 100 ug mL stock solution The concentration of the 5 standard solutions is 1 2 5 10 and 20 ug mL The volume of the 5 solutions is 50 mL www promochrom com 22 PromoChrom Instruments LC 07 Syringe pump 1 Set up the device as following solvent WW Stock Standard solutions solution 2 Build a method as shown in the following table only necessary for the first time Remarks Fill the tubing between valve and stock solution Fill the tubing between valve and solvent Add stock solution for 20 ug mL standard Add solvent for 20
28. ug mL standard to make 50 mL Add stock solution for 10 ug mL standard Add solvent for 10 ug mL standard to make 50 mL Add stock solution for 5 ug mL standard Add solvent for 5 ug mL standard to make 50 mL Add stock solution for 2 ug mL standard Add solvent for ug mL standard to make 50 mL Add stock solution for 1 ug mL standard Add solvent for 1 ug mL standard to make 50 mL 3 Press Start button The device will complete all the preparation work in around 10 minutes 1 2 Addition of reagents to a chemical reactor Timing and speed of adding reagents to a chemical reactor are critical to the yield of a chemical reaction These two factors can be controlled automatically using a LC 07 Sometimes a multi step synthesis need to change the conditions drastically For example a synthesis need to be carried out at 5 C for first reaction and then change temperature to over 100 C for the second reaction This change may be achieved automatically also using a LC 07 and two reactors Below is a demonstration of the setting which involves addition of 4 reagents and two reaction stages The reactor 1 is maintained at low temperature and reactor 2 is maintained at high temperature www promochrom com 23 PromoChrom Instruments LC 07 Syringe pump 2 Specifications Number of valve ports 10 or 18 Delivery volume mL 0 5 5000 Flow rate mL min 0 1 90 Delivery reproducibility C V lt 0 5 Delivery accuracy l lt Di
29. y and user manual SPE 03 03 MOD 001 Includes 6 channel SPE 03 mainframe 24V power supply and user manual Module for online fraction evaporation PromoChrom Instruments SPE 04 www promochrom com 10 SPE 04 multi functional solid phase extraction system SPE 04 multi functional solid phase extraction system is a flexible and versatile platform for automatic sample preparation It can perform multiple tasks offline solid phase extraction online solid phase extraction normal sample injection and online derivatization with controlled temperature It is designed for automatic cleanup of biological samples such as small molecules in plasma and urine samples Compared to SPE 01 SPE 04 has smaller sample volume and fraction volume and can process much larger number of samples per batch 1 Working principle of SPE 01 The following diagrams describe the structure and typical working procedures of SPE 04 The plunger for SPE column can seal the column well It can work with SPE columns from most suppliers There 1s no need for a special cap or adapter Wash Change a Condition Load needle o new PE with 1 or 2 o sample cartridge solvents Typical Collect fraction Elute with working Offline SPE 1 2 solvents proce dures Inject to HELE Derivatize with lore Collect fraction trigger to start i reagents at controlled Online SPE HELO temperature Optional 2 Features 2 1 Multi funtional platform
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