H201-NIKON-TI-S-ER
Contents
1. www oko lab com De H201 NIKON TI S ER User Manual November 2014 2 60mm Petri dish 4 35mm Petri dish 1 1x3in chamber slide and 2 35mm Petri dish 1 Lab Tek 1in x2in chambered cover glass and 2 35mm Petri 1 Lab Tek Il 1in x2in chambered cover glass and 2 35mm Petri 1 Lab Tek 1in x2in chambered cover glass and 1 60mm Petri 1 Lab Tek Il 1in x2in chambered cover glass and 1 60mm Petri 1 1x3in chamber slide and 1 60mm Petri dish 1 60mm Petri dish and 1 35mm Petri dish Magnetic lock to hold the standard MW plates in the chamber Magnetic lock to hold the low profile MW plates in the chamber gt gt gt GS35 M 2x60 M GS60 M 1x60 M LABTEK 60 M For 1 1 x3 chamber slide and For 4 35 mm For 2 60 mm For 1 60mm petri dish and For 1 60 mm For 1 Lab Tek 1 x2 chambered 2 35mm petri dishes petri dishes petri dishes 1 1 x3 chamber slide petri dish cover glass and 1 60mm petri dish 1x35 M 6035 M 1xGS M 2xGS M 1xGS 2xLABTEK M 1xLABTEK M For 1 35 mm For 2 35 mm petri dishes and For 1 1 x3 For 2 1 x3 For 1 1 x3 chamber slide For 1 Lab Tek petri dish 1 60mm petri dish chamber slide chamber slides and 2 Lab Tek 1 x2 1 x2 chambered chambered cover glass over glass P LABTEK 35 M LABTEK 60 M 1xLABTEK M 2xLABTEK M LABTEK II 35 M 2xLABTEK II M For 1 Lab Tek 1 x2 For 1 Lab Tek 1 x2 For 1 Lab Tek 1 x2 For 2 Lab Tek For2. Temperature Sensor dedicated opening 1 Frontal view 2 3D view Figure 8 Insertion of the temperature sensor inside the chamber 5 Insertion of Sample Holder into Chamber Sample holders fit into the chamber base and are held in place by magnets embedded within both chamber and hold er To introduce a sample holder with the proper orientation match the red dot on the holder to the one on the chamber base as illustrated in Figure 9 Figure 9 Introduction of the Specimen Holder inside the Chamber Base www oko lab com pase H201 NIKON TI S ER User Manual November 2014 6 Working with 35 or 60 mm Petri Dish Spacing Rings and Magnetic Locks Magnetic locks prevent movement of 35 and 60 mm dishes inside the sample holder Figure 10 illustrates the available magnetic locks for 35 and 60 mm dishes Threaded magnetic posts allow adjusting holder s height Figure 10 Magnetic locks for 35 and 60 mm dish NOTE Magnetic locks are included with sample holder Spacing rings accounting for variability in dish diameter from one manufacturer to another are also included Figure 11 shows how to position the ring Ring selection guide diagrams rings for 35 and 60 mm dish Figure 12 and Figure 13 respectively Figure 11 1 Insertion of the ring 2 insertion of the 35 mm dish 3 insertion of the magnetic lock NOTE Spacing rings are included with sample holder www oko lab com sie H201 NIKON TI
3. 15 www oko lab com NG H201 NIKON TI S ER User Manual November 2014 1 Components and dimensions _ H201 NIKON TI S ER includes the following components Chamber main body It connects to the adapter with screws Sliding glass lid for easy pipetting Chamber riser It is a removable frame increasing the height of the chamber from 24 to 30 mm often re quired when using multi well MW plates Adapter It connects to the Nikon XY Stage TI S ER with screws Sliding Lid Distance between focal plane 3 and upper side of the glass lid with the chamber riser 0 Distance between focal plane 24 and upper side of the glass lid without the chamber riser Sample Holder Order Separately Chamber Base Chamber top view Adapter 155 Figure 1 H201 NIKON TI S ER Components and Dimensions 2 Sample Holders 2 1 Available Sample Holders The following sample holders are available NOTE Please contact info oko lab com if you cannot find the sample holder you are looking for We are constantly adding new inserts to the list 1 35mm Petri dish 1 1x3in chamber slide 1 Lab Tek 1in x2in chambered cover glass 1 Lab Tek II 1in x2in chambered cover glass 1 60mm Petri dish 1 Nunc and Greiner T25 flask 2 35mm Petri dish 2 1x3in chamber slides 2 Lab Tek 1in x2in chambered cover glass 2 Lab Tek Il 1in x2in chambered cover glass
4. S ER User Manual November 2014 2 BD Falcon 35x10 Willco 35x10 Se 2 e Figure 12 Ring selection scheme for 35 mm dish Grainer Petri35x10 Ibidi p Dish 35 mm low e Corning 35x10 mm Corning 60x15 mm www oko lab com MatTek P35G X14 X MatTek P50G X30 X Figure 13 Ring selection scheme for 60 mm dish Willco 60x15 Grainer Petri 60x15 jol page LO H201 NIKON TI S ER User Manual November 2014 7 Working with 1x3 and 1x2 chamber slides magnetic locks Magnetic locks prevent movement of 1 x 3 and 1 x 2 chamber slides inside of the sample holder NOTE Magnetic locks are included with sample holder Figure 15 Magnetic lock for 1 x 2 chamber slide To lock the slide push simultaneously the buttons indicated with 3 1 www oko lab com H201 NIKON TI S ER User Manual November 2014 8 Working with MW Plates Magnetic locks and Chamber ris er Magnetic locks hold MW plates in place into sample holder illustrated in Figure 16 NOTE Magnetic locks must be ordered separately NOT INCLUDED Product codes MW LOCK 22 5 and MW LOCK 16 5 w u iu 0 ei ees BEER N d MW 22 5 LOCK To be ordered separately Typically used for 6 12 24 48 MW Figure 16 Magnetic locks for MW plates To be ordered separately Chamber Riser increases chamber s height and is REQUIRED with 6 12 24
5. 1 Lab Tek Il For 2 Lab Tek Chambered cover glass Chambered cover glass Chambered cover glass 1 x2 chambered 1 x2 chambered cover glass 1 x2 chambered cover glass and 2 35mm petri dishes and 1 60mm petri dish cover glass and 2 35mm petri dishes Figure 2 Available sample holders The following glass Lids are available for H201 NIKON TI S ER H201 SLIDING LID For easy sample loading and pipetting Included in code H201 NIKON TI S ER H201 KOEHLER LID Reduces chamber height to 21 mm and allows imaging under Koehler illumination H201 INJECTION LID Glass lid with two small openings sealed with flexible plastic allowing injection or permanent access to the sample Compatible only with sample holder 1x35 H201 LASER INTERLOCK LID Glass lid with safety switch Connects to laser controller and automatically turns laser off when lid is lifted Compatible only with sample holder 1x35 OPTIONAL not included with H201 NIKON TI S ER www oko lab com Page H201 NIKON TI S ER User Manual November 2014 3 1 Sliding lid The Sliding Lid is a glass lid allowing for easy sample loading and pipetting The Sliding Lid is screwed onto the cham ber Figure 3 shows chamber dimensions with the Sliding Lid with and without chamber riser Figure 4 illustrates how to remove Sliding Lid when a different Lid is necessary NOTE Sliding Lid MUST BE REMOVED when using any other Lid The Sliding Lid is fixed onto the chamber w
6. 48 MW plates regardless of the Lid em ployed Figure 17 shows how to install and fasten the chamber riser in place Screws location is indicated by letter A Chamber Riser Figure 17 Riser Assembly www oko lab com el 2 H201 NIKON TI S ER User Manual November 2014 9 Connection of the Gas Supply A single silicon tubing carries output gas from the Okolab Gas Controller to the H201 NIKON TI S ER Silicon tubing connects to a gas input brass opening located on a corner of the H201 NOKON TI S ER See Figure 18 Connect by gently pushing silicon tubing onto brass opening Gas Input Figure 18 Connection with gas supply 10 Working with Perfusion The Chamber Riser included with H201 NIKON TI S ER features 12 perfusion holes for the insertion of perfusion tubing up to 2 5 mm in outer diameter Small screws plug the perfusion holes when not in use Grub screws M3x6 Remove small screws as needed before introducing perfusion tubing Figure 19 shows location of perfusion holes Perfusion dedicated opening 1 Frontal view 2 3D view Figure 19 Perfusion 11 Connection of the Chamber with adapter Follow the steps shown in the images of Figure 20 and listed below in order to correctly install the chamber onto XY stage 1 Follow the arrows shown in Figure 20 Images 1 and 2 for placing the captive screws in the proper housings 2 Place the chamber on the ad
7. H201 NIKON TI S ER User Manual November 2014 H201 NIKON TI S ER Compatible with the following XY stages Compatible with the following Okolab Controllers Nikon Ti S E and Ti S ER H201 T UNIT BL www oko lab com Page 1 H201 NIKON TI S ER User Manual November 2014 Index 1 Components and dimensions sus 3 2 3Sample iHolders see ses Se ee ree 3 2 1 Available Sample Holders 2 020 3 3 MVallablelidsceiseete ee ee ee ee ee ee Eee E ee ee ee 4 3 1 SUOMI ee een ee eier 5 3 2 Koehler Lid a a a a 6 3 3 ecn idee te ee te ee ee er Gee mrs Gears Gears Gers en cea cesees 6 3 4 L ser Interl ck Lid une 7 4 Insertion of the Sample Feedback Temperature Sensor una a 8 5 INSertion Of Sample Holder into Chamber a sera 8 6 Working with 35 or 60 mm Petri Dish Spacing Rings and Magnetic LOCKS ccccceecccccccccscccesccceccs 9 7 Working with 1x3 and 1x2 chamber slides magnetic lOCKkS seseeseseecesoecesoecesoeceseecesoeceseeceseeoeo 11 8 Working with MW Plates Magnetic locks and Chamber riser cccccccccccccccccccccccccecccecccecccecccecees 12 9 Connection of the Gas Supply u nee 13 10 Working with Perfusion u 13 11 Connection of the Chamber with adapter esesesesesesescesececececesesceecesecececeseseseecesececesesesesescecese 13 12 Connection of the Chamber with XY stage sesescecescecesoecesoecesoecesoecesoecesoecesoeceseeceseeceseeceseeceo
8. apter as shown in Figure 20 Image 3 3 Use a 1 5mm metric Allen Wrench to tighten two of the four captive screws in place A in Image 4 while keeping the sliding lid closed See Figure 20 Image 4 www oko lab com bape 3 H201 NIKON TI S ER User Manual November 2014 4 Tighten the remaining two captive screws A in Image 5 while keeping the sliding lid open 5 Image 6 of Figure 20 shows the chamber fixed on the adapter Captive Screw Figure 20 How to assemble the chamber to the adapter www oko lab com page LA H201 NIKON TI S ER User Manual November 2014 12 Connection of the Chamber with XY stage Once you have assembled the chamber with adapter you can place it on the XY stage following the steps shown in Figure 21 1 Place the adapter on the stage See Figure 21 Image 1 2 Use the Grub screws to align correctly the adapter on the stage Grub screws housings are indicated with letter A in Image 2 of Figure 21 3 Follow the arrows shown in Image 3 of Figure 21 for placing flat socket head cap screws use the provided Allen wrench for tightening the flat socket head cap screws and fastening the adapter on the stage Screws housings are indicated with letter B in Image 4 of Figure 21 Screw M3x3 ng a Figure 21 How to fix the H201 NIKON TI S ER to the XY stage www oko lab com 5
9. ith 4 screws Screws location is indicated by letter A in Figure 4 image 2 and 3 Keep Sliding Lid fully closed to access screws labeled A in image 2 Open Sliding Lid to access screws labeled A in image 3 Distance between focal plane and upper side of the glass lid with the chamber riser Distance between focal plane and upper side of the glass lid without the chamber riser Chamber top view lt gt 1 Si Figure 4 Assembly of the sliding lid www oko lab com Be H201 NIKON TI S ER User Manual November 2014 3 2 Koehler Lid The Koehler Lid is a glass lid reducing the chamber s height to 21 mm and allowing imaging under Koehler illumination The Koehler Lid is placed onto the chamber NO SCREWS or TOOLS are necessary Figure 5 shows chamber dimensions with Koehler lid with and without chamber riser H201 KOHLER LID 27 Distance between focal plane and upper side of the glass lid with the chamber riser Distance between focal plane 2 and upper side of the glass lid without the chamber riser Chamber top view 109 70 Figure 5 H201 NIKON TI S ER H201 KOEHLER LID 3 3 Injection Lid The Injection Lid is a glass lid with two small openings sealed with flexible plastic allowing injection or permanent access to the sample NOTE Compatible with sample holder 1x35 ONLY The Injection Lid is placed onto the chamber NO SCREWS or TOOLS are necessary Figure 6 shows cha
10. mber dimensions with the Injection Lid with and without chamber riser s www oko lab com H201 NIKON TI S ER User Manual November 2014 28 Distance between focal plane and upper side of the glass lid with the chamber riser Distance between focal plane 22 and upper side of the glass lid without the chamber riser Chamber top view Figure 6 H201 NIKON TI S ER H201 INJECTION LID 3 4 Laser Interlock Lid The Laser Interlock Lid is a glass lid with a safety switch connecting to the laser controller It automatically turns the laser off when the lid is lifted NOTE Compatible with sample holder 1x35 ONLY The Laser Interlock Lid is placed onto the chamber NO SCREWS or TOOLS are necessary Figure 7 shows chamber dimensions with the Laser Interlock Lid with and without chamber riser H201 LASER INTERLOCK LID 29 Distance between focal plane and upper side of the glass lid with the chamber riser Distance between focal plane 23 and upper side of the glass lid without the chamber riser 0 Chamber top view 09 70 Figure 7 H201 NIKON TI S ER H201 LASER INTERLOCK LID www oko lab com ew H201 NIKON TI S ER User Manual November 2014 4 Insertion of the Sample Feedback Temperature Sensor Insert the Sample Feedback Temperature Sensor through the dedicated opening located in the H201 NIKON TI S ER see Figure 8 Front and 3D views Temperature Sensor dedicated opening
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