User manual
Contents
1. Dar 3 BIOMETRA Lid temp 109 0C Preheating On Info Page i Save Pgm i EMEGE Note The default setting which is recommended for most applications is preheating On After all pre settings have been made press Enter to open the programming spreadsheet 4 6 Enter temperature and time settings The programming spreadsheet Temp C Time lt Options gt L 2 33 4 Info Insert Del Save Pgm Enter Note In this spreadsheet you can enter all parameters for your cycling protocol In addition you can set special parameters like touch down ramping rates or time increments For details on these special parameters see section 5 1 cursor keys lt gt You can navigate in the spreadsheet without limitations with the four Note Each setting is confirmed with Enter The cursor moves automatically to the next field Alternatively you can confirm a value by moving forward with the cursor keys Note At any time you can call up the help function with Info Note In an existing protocol program steps can be deleted and inserted For further information on deleting and inserting program steps see section 5 1 Whatman 14 Now enter temperature for the first step an press Enter In the next row you can enter the time for this temperature Temp C Time Options gt Te 9940 23 33 4 Info Insert Del Save Pgm Enter Note T2. To unlock wheel press down metal pin with a ball pen and turn wheel carefully This pin overcomes the automatic clutch mechanism Thus care must be taken not to apply excessive pressure Whatman T3000 Manual Lid arrested in upper position carefully turn wheel with pressed pin CLOCKWISE until you feel normal resistance no more clicking noise clutch is released Release pin and turn lid down until the clutch mechanism is activated clicking noise optimum pressure applied Lid arrested in down position carefully turn wheel with pressed pin COUNTER CLOCKWISE until you feel normal resistance no more clicking noise clutch is released Release pin and turn wheel counter clockwise until pressure is completely released Open lid 1 IMPORTANT WHEN THE CLUTCH MECHANISM IS ACTIVE OPTIMUM PRESSURE IS APPLIED DO NOT USE PIN TO FURTHER INCREASE PRESSURE THIS MAY LEAD TO DAMAGE OF TUBES AND INSTRUMENT Whatman 10 4 Create program Prior to writing a new program a program store has to be chosen The T3000 Thermocycler provides 10 subdirectories of which each can store up to 99 different programs For easy identification of the selected storage place the subdirectories can be named see section 4 1 Select directory Start with the main screen Prog Step Temp 23 9C 23 8C 23 9C Time Lid 24 0C 23 9C 23 9C AFG System Start Stop Erelhiie
3. Block 2 active Pause Stop Back Block 3 inactive Start mmes Move cursor to desired block and press Enter Info Pause Block 2 active Pause Stop Back SEORB Press Pause to pause block Press Stop to stop block Prog Step Temp Time Lid Info 20 3 20 2 System 1 3 Biometra 99 0399 0C Pause 109 0 Start Stop 20 3 20 2 Hdit Note The program pauses in the current state The word Pause is displayed and alternates with the elapsed time You can continue the run with Start Stop Whatman 28 8 System settings Main screen Prog Step Temp 23 9C 23 8C 23 9C Time Lid 24 0C 23 9C 23 9C TATO System Start Stop TELE To change instrument settings press System System Info Signal Language Sprache Display contrast Diagnostics Page NY Back Enter Owe U NE Scroll though the list with the cursor keys 1 VW Enter selected function with Enter 8 1 System Info Select System info Press Enter T3000 Thermocycler Serial Number 123456 Block type Vers 0 51t3p Biometra 2004 TALO Cancel Enter This page shows the serial number of the instrument and software version 8 2 Switch beep on off There is an option for an audible signal whenever a program enters a pause and when the program is finished If this option is a
4. Press Press Edit to enter the edit mode oO edit program O 1 7 2 C 3 I 4 Page T Name Back Bnieeie You are now in the directory structure of the edit mode Use cursor keys to select a subdirectory edit program l SS wWN FO Page it Name Back Enter Press Enter to enter highlighted subdirectory The program storages are displayed 3 B 0 empty B 1 empty Edit program EB 2 empty B 3 empty B 4 empty Page T Page Copy Del Edit Whatman T3000 Manual 11 4 2 Select program store lt gt Use cursor keys to select a program store ale Zu Gt u 5 pa Tip Use numeric keypad to directly enter program number an oa On For numbers smaller than 10 enter 0 plus number BE O za EI B 22 B 0 empty B 1 empty Edit program B 2 empty B 3 empty B 4 empty Page N Page Vv Copy Del Edit Using the softkeys Page and Page V you can scroll through the list in 5 program steps Press Edit to write new program in Program store 03 4 3 Enter program name Each program is specified by a program number and a subdirectory number To make retrieval of a program easier you can enter a name for each program existing of letters numbers and symbols ae
5. Options gt 24 Back Stane To run program press Start Whatman 26 7 3 Display during operation During preheating of the lid the following screen is displayed Prog 3 Biometra Step 1 Temp 20 3 99 0299 0C 20 3 Time Oh 1ml3s Lid 20 2 109 0 20 2 Info System Start Stop Edit Note While lid preheating the block is held constantly at 25 0 C The program starts as soon as the lid has reached the set temperature During the preheating phase the display of the lid temperature alternates between the current lid temperature and preheating 7 4 View remaining run time Display during operation Prog 3 Biometra Step 1 Temp 20 3 99 0299 0C 20 3 Time Oh 1m13s Lid 20 2 109 0 20 2 Info System Strane MooR Toit To view remaining run time press Info Info Block I BILOEK 2 Block 3 Dir 3 Prog 3 BIOMETRA Remain 1h13m Status Inactive Active Inactive Back The current remaining run time id displayed in line 4 Remain 7 5 Pause stop program Display during operation Prog 3 Biometra Step 1 Temp 20 3 99 0299 0C 20 3 Time Oh 1m13s Lid 20 2 109 0 20 2 Into System Start Stop Edit To pause or stop one of the three blocks press Start Stop Whatman T3000 Manual 27 TALGO Block 1 inactive Start Direct
6. 3 4 Initial self test After switching on the T3000 the serial number of the instrument and the software version is displayed T3000 Thermocycler Serial number Block type Vers c Biometra 2004 The T3000 then checks all programs in all subdirectories so called RAM check T3000 Thermocycler Serial number Block type Checking directory 7 program 4 3 5 The T3000 display The T3000 display provides information about the instrument and about the status of the active program Prog Step Temp 23 9C 23 8C 23 9C Time Lid 24 0C 23 9C 23 9C Info System Start Stop Belit Softkey 1 Softkey 2 Softkey 3 Softkey 4 The function of the four soft keys beneath the display is context sensitive The respective function is shown in the display The function of each key can differ from screen to screen 3 6 Navigation within the T3000 software The T3000 thermocyclers provides spread sheet programming This means that all program parameters are entered into a simple central spreadsheet Four navigation keys provide easy navigation within the software The back and the forward key have additional functions as described below Whatman The right cursor key moves the cursor to the next field lt gt This cursor can also be used to complete data entry By pressing the right cursor settings will be saved and the cursor moves to the next field In the file direct
7. 1 Before you start Safety precautions Please read this manual carefully before starting operation of the T3000 Thermocycler General safety precautions for laboratory work must be observed when working with the T3000 thermocycler Be aware that samples are reaching high temperatures Do not touch or open hot tubes or microplates because hot liquid may quickly spill out Do not heat samples without having the lid locked securely Before opening of the lid release lid pressure see section 3 7 Do not place fingers between lid and housing when opening or closing the lid Do not touch the heated lid When only few samples are put in the block place additional tubes in the four corner positions This is to evenly distribute the lid pressure and prevents single tubes from excessive pressure Use of few tubes may result in damage of the tubes by excessive pressure Use only suited plastic ware in the T3000 thermocycler Tubes and plates must show good fit when placed in the thermoblock Only use tubes that are suited for high temperatures tight lids The T3000 Thermocyler contains no user serviceable parts Do not open the housing instrument Service and repair may only be carried out by the Biometra Service department or otherwise qualified technical personal Do not use the instrument when damages of the housing block cable or other parts are visible The T3000 Thermocycler must not be used with explosive flammable or vol
8. 1C 22 0C LiMIE System Start Stop EELE Note To view the remaining runtime during a run press Info in the main menu Whatman T3000 Manual 17 5 Edit programs Select program as described in section 4 2 Note During operation of the cycler the active program can be viewed but not modified If you want to change settings of the active program for further experiments you have to save a copy of this program to another memory For further information about copying programs see section 3 3 5 1 Delete program steps The programming spreadsheet Temp C Time lt Options gt ds 99 0 Oh 5m Os 2 99 0 Oh Im Os 3 55 0 Oh Im Os 2 24 4 34 0 Oh 1m Os Info Insert Del Save Pgm Enter To delete a program step press Insert Del Insert Delete step L 99 0 Oh 5m Os 2 99 0 Oh 1m Os 3 55 0 Oh Im Os 2 24 4 34 0 Oh 1m Os Del Cancel Delete Insert Enter the number of the step that you want to delete Insert Delete step 8 ite 99 0 Oh 5m Os 2 99 0 Oh 1m Os 3 55 0 Oh Im Os 2 24 4 34 0 Oh 1m Os 4 Del Cancel Delete Insert Press Delete Insert Delete step 8 1 99 0 Oh 5m Os 2 99 0 Oh 1m Os 3 55m0 Oh 1m Os 2 24 4 34 0 Oh 1m Os gt gt gt Deleting step 3 lt lt lt Whatman 18 Temp C Time lt Options gt L 99 0 Oh 5m Os 2 99 0 Oh 1m Os 33 34 0 Oh 1m Os 4 4 0 Paus
9. can scroll the display to the right Move the cursor to the right side row of the display Options gt Main funct aT C dt s c s Le 3 00 23 3 00 33 3 00 4 3 00 Ini Insert Del Save Pgm Enter Note To go back to programming sheet move the cursor to the very left side of the display row Main funct Alternatively you can move the cursor to the very right Temp C Time Options gt T4 99 0 Oh 5m Os 2 99 0 Oh 1m Os 3 55 0 Oh Im Os 2 24 4 34 0 Oh 1m Os Info Insert Del Save Pgm Enter Note In the main screen you will find an information that a special parameter has been modified This is indicated by a in the row labelled with opt gt 6 1 Program time increments To compensate for loss in enzyme activity each step within a loop can be extended from cycle to cycle Enter the desired time increment seconds in the row labelled with dt s This value will be added to the time value from cycle to cycle Main funct aT C dt s C s L 3 00 23 3 00 33 10 3 00 4 3 00 Info Insert Del Save Pgm Enter Whatman T3000 Manual 23 Note A time increment will have an impact on the total runtime depending on the numbers of cycles and the size of the increment A program with many cycles and large time increments will take a significantly longer time than a standard protocol 6 2 Program touch down
10. ea 3 A ABCDEFGHIJKLM NOPQRSTUVWXYZ C O amp Blank Del Name OK Enter lt gt Use cursor keys to select a letter Whatman 12 Accept letter with Enter II 3 3 E A ABCDEFGHIJKLM NOPQRSTUVWXYZ C O amp Blank lt Del Name OK Enter Z lt 4 gt Use cursor keys to select next letter Z Accept letter with Enter Repeat until name is completed 0 ee 5 3 BIOMETRA A ABCDEFGHIJKLM NOPQRSTUVWXYZ C Q amp Blank Del Name OK Enter Once the name is completed confirm name with name OK San eee B 3 BIOMETRA Lid temp C Preheating On TATO Page I Save Pgm Enter 4 4 Enter lid temperature You can now enter a temperature for the heated lid Note In general the lid temperature should be about 10 C above the highest temperature in the protocol Example If your denaturation step is at 99 C enter 109 C for the lid a ea B 3 BIOMETRA Lid temp 109 0C Preheating On Info Page J Save Pgm Enter Whatman T3000 Manual 13 4 5 Select deselect lid pre heating You can choose whether the lid is pre heated before the program starts This is to avoid condensation during the initial heating phase Note During the preheating of the lid the block is held constant at 25 C
11. gt gt gt Copying program lt lt lt I 2 B 0 empty B 1 empty Edit program B 2 empty EB 3 BIOMET2 B 4 empty Page N Page Vv Copy Del Edit 5 4 Delete program Select program as described in sections 4 1 and 4 2 Whatman T3000 Manual Fl mr B 0 TESTI B 1 TEST2 Edit program B 2 BIOMET B 3 BIOMET2 B 4 BIOMET3 Page N Page Vv Copy Del Edit Press Copy Del 3 B 3 BIOMET2 Copy Pgm Delete Pgm Back To delete this program press Delete pgm II 3 B 3 BIOMET2 Delete program Cancel Delete Note Once a program has been deleted it cannot be restored Press Delete gt gt gt Deleting program lt lt lt In the directory structure the program is displayed as empty Eee B 0 TESTI E 1 TEST2 Edit program B 2 BIOMET 3 empty B 4 BIOMET3 Page N Page Vv Copy Del Edit You can enter a new program To get back to the main screen 2 x press cursor key lt 21 Whatman 22 6 Further programming options Temp C Time lt Options gt 1 99 0 Oh 5m Os 2 99 0 Oh 1m Os 33 55 0 Oh Im Os 2 24 4 34 0 Oh 1m Os Info Insert Del Save Pgm Enter Note To program special parameters you
12. 0 0200000000 0000 000000000 33 12 Order information sosssosssoossonssonsssonssnnnsnnnsnnnssonnsnnssnnnsnnnsnnnssnnssnnnsnnnssnnssnnssnnsnnnssnnnsnnnee 34 WS UT 8 1 11 a ccivcescsscscssscscsccsstvacesssecsesccepesscesssessesceedsosesecsedeescsacedcasosstunesesecesessodseescsestedessvoeseoasesess 35 14 Equipment Decontamination Certificate scnsssssesssonsensonsensssnsnonnensonnsnnsssssnnnnnsonnsnnnsnssnne 36 Whatman T3000 Manual 1 1 Introduction The T3000 Thermocycler represents the technological upgrade of one of the most successful Thermocyclers in Biometra s history Utilising the popular triple block concept the T3000 has been equipped with the latest in Peltier technology to achieve excellent heating and cooling rates In addition the software has been completely revised for improved ease of use High performance on a small footprint combined with unlimited flexibility this is what makes the new T3000 Thermocycler the ideal instrument for all laboratories where flexibility and high performance is needed 1 1 Three independent Thermocyclers in one instrument The T3000 Thermocylcer offers three independent blocks in one housing thus different protocols can be run at the same time This makes the T3000 Thermocycler the perfect instrument for laboratories where high flexibility is needed With a maximum capacity of 3 x 48 wells the T3000 also offers high throughput in parallel operation The T3000 Thermocycler is ava
13. For some applications it is useful to start with a higher annealing temperature and to decrease the temperature from cycle to cycle This subsequent lowering of the annealing temperature is called touch down To decrease the annealing temperature from cycle to cycle enter a negative temperature increment in the row labelled with dT s Main funct dT C dt s Al s Ls 3 00 23 lt 3 00 3 lt 0 10 3 00 4 3 00 LIES Insert Del Save Pgm Enter Note Be sure that the temperature decrease is set in a step that lies within a loop Otherwise there will be no iterative temperature decrease 6 3 Adjust heating and cooling ramps Since the T3000 is a fast thermocycler it may be necessary to reduce the heating and cooling ramp for some applications For example this is helpful if protocols from other Thermocyclers shall be used The heating or cooling ramp respectively can be set in the row labelled with A s Main funct aT C dt s Al s ls 3 00 2 lt 1 00 3 3 00 4 3 00 Info Insert Del Save Pgm Enter Note The default setting 3 00 s indicates maximum ramping Whatman 24 7 Run program 7 1 Select and start program Main display Prog Step Temp 23 9C 23 8C 23 9C Time Lid 24 0C 23 9C 23 9C marO System Start Stop Henie To run a program press Start Stop Block 1 Block 2 Block 3 Inactive inac
14. Whatman T 3000 hermocycler User manual June 2004 050 720 T3000 Thermocycler 20 050 723 T3000 Thermocycler 48 050 724 T3000 Thermocycler combi Whatman T 3000 hermocycler User manual June 2004 Order No 050 720 T3000 Thermocycler 20 050 723 T3000 Thermocycler 48 050 724 T3000 Thermocycler combi Biometra biomedizinische Analytik GmbH Rudolf Wissell Str 30 D 37079 Goettingen Tel 0551 50 686 0 Fax 0551 50 686 66 email biometra info whatman com http www biometra com 1 Introduction iesssssssssusenassasssasssnnnnsnnesnsssennesnnesunsesanssnnnsnnsnsnseshsssensssnseshnsernssenstensensesenesseunsann een 1 1 1 Three independent Thermocyclers in one instrument uenseeseessersenssensennnnnnnnennennnennnnn 1 1 2 Easy programming 0 ccceecessseessessscesseeeseeescessseessecsseceseeceseccseesseceseeeeeseeseceseeseeseneeeeseeeseeeas 1 1 3 Higher speed eines aaa RE a Rudi 1 1 4 Smart lid technology misisse arsino a aiaa 1 2 Before VOU start sccciicccdecciesssecsenuecccesasssessesacesessncassoadeseceseasasansanssseascoassavasenssecsosonssaeasensesanesaesee 2 2 1 Safety precautions ssc sc stdciecdececasevedenticcdadadecivs eeeandevete Went kai el 2 2 2 T3000 Thermocycler installation ccccecscsesssesseeeseeescessseceeceeceseeseseceeeseeseeeeseseneeenseees 3 3 First steps with the T3000 cssscsssccssssscccsecssessccescsscscessesssccenssssscssscssscssescssscssscesseees 4 3 1 Th
15. ase keep the original packaging material for return shipment in case of servicing The T3000 carton provides a specially developed system for contact free transport of this electronic device The display contrast can be adjusted to local lightning conditions see section 8 4 Before connecting the unit to the power source please ensure that the voltage selector at the back side of the instrument is set to the required voltage Danger of electric shock Unplug the power cable before you open the T3000 Thermocycler Ensure that both the rear and bottom ventilation slits of the rear and bottom of the instrument are unobstructed Insufficient ventilation can cause overheating of the instrument Whatman 3 First steps with the T3000 3 1 The T3000 Thermocycler front view wheel for lid pressure adjustment Lid release bolt Graphical display Ventilation slots Power switc Whatman T3000 Manual 5 3 2 The T3000 Thermocycler rear view 27 A holder m 2 x TT 4A see type label RS232 serial port Power connection data interface Whatman 3 3 The T3000 control panel Block status Graphical LED display Block 1 Im Bibck 2 5 Block 3 5 inv A 209 qf k Je Je tee LL Je JE T 3000 T3000 Power switch Navigation keys Softkeys context sensitive Whatman T3000 Manual 7
16. atile liquids Appropriate safety regulations must be observed when working with infectious or pathogenic material The thermoblock and the heated lid will reach high temperatures during operation Both thermoblock and heated lid can burn you Rapid heating of the thermoblock can cause liquids to boil explosively Always wear safety goggles during operation Close the lid before starting a program It is not necessary to apply oil into the opening of the block in order to improve the heat transfer between the block and the sample tubes gt PD If you still decide to use oil do not use silicon oil Mineral oil may be used Whatman T3000 Manual 2 2 T3000 Thermocycler installation Place the T3000 Thermocycler on a stable surface in a dry environment Let equilibrate the T3000 Thermocycler to room temperature before starting operation 1 to 6h Make sure that the ventilation slots on the bottom and the rear are not obstructed see section 3 2 There must be sufficient distance between the ventilation slots on the rear side of the Thermocycler and a wall or another instrument min 10 cm Make sure that there is no object underneath the thermocycler that may block the ventilation slots on the bottom e g a piece of paper etc Make sure that the main supply voltage is in accordance with the label above the power connection see section 3 2 Connect the T3000 Thermocycler to a grounded socket Ple
17. consumption 420 Watt Noise emission Very low Interfaces Serial RS232 port data interface Dimensions W x D x H 30 cm x 38 cm x 19 cm Weight 11 9 kg Working conditions 5 35 C 70 relative humidity According to Biometra standard procedure Technical specifications are subject to change Whatman 34 12 Order information Order No T3000 Thermocycler 20 050 720 T3000 Thermocycler 48 050 723 T3000 Thermocycler combi 050 724 For information about tubes strips microplates and other consumables please contact BIOMETRA or your local BIOMETRA distributor Whatman T3000 Manual 35 13 Warranty This Biometra instrument has been carefully built inspected and quality controlled before dispatch Hereby Biometra warrants that this instrument conforms to the specifications given in this manual This warranty covers defects in materials or workmanship for 24 months as described under the following conditions This warranty is valid for 12 month from date of shipment to the customer from Biometra or an authorised distributor This warranty will not be extended to a third party without a written agreement of Biometra This warranty covers only the instrument and all original accessories delivered with the instrument This warranty is valid only if the instrument is operated as described in the manual Biometra will repair or replace each part which is return
18. ctive the beep can be switched off by pressing any key of the keyboard Select Signal Press Enter Whatman T3000 Manual 29 With signal Yes NO LALO Cancel Enter 8 3 Select language The display of the T3000 can be switched from English to German Select Language Sprache Press Enter Language Sprache German Deutsch English Englisch Info Cancel Enter To confirm your selection press Enter 8 4 Display contrast The display contrast can be set in 4 levels to achieve optimum visibility under individual light conditions Select Display Contrast press Enter Contrast 0 4 or arrows 2 TORO Cancel Enter User cursor keys to in decrease contrast value or directly enter setting The default setting is 2 which is meets average conditions 8 5 Diagnostics The diagnostic screen contains internal information used by the Biometra service department for servicing Whatman 30 8 6 Temperature readout Each T3000 block can be equipped with an in tube temperature sensor 050 408 for 0 2ml wells 050 409 for 0 5ml wells This sensor is to monitor the temperature inside a test tube The temperature of the in tube sensor can be shown in the T3000 display The sensor display will replace the display of the lid temperature Temperature read out Heated lid Tube Sensor Cancel Enter Note The in tube senso
19. d repair The T3000 Thermocyler contains no user serviceable parts Do not open the housing instrument Service and repair may only be carried out by the Biometra Service department or otherwise qualified technical personal Whatman 32 10 Instructions for return shipment In case of an instrument failure that cannot be fixed by the procedures described in section 9 2 please proceed as follows Return only defective devices For technical problems which are not definitively recognisable as device faults please contact the Technical Service Department at Biometra Tel 49 551 5068640 Fax 49 551 5068666 e mail biometra info whatman com Important Carefully clean all parts of the instrument of biologically dangerous chemical or radioactive contaminants If a an instrument is contaminated Biometra will be forced to refuse to accept the device The sender of the repair order will be held liable for possible losses resulting from insufficient decontamination of the device Please prepare written confirmation that the device is free from biologically dangerous and radioactive contaminants The declaration of decontamination see 14 has to be attached to the outside of the packaging Use the original packing material If not available contact Biometra or your local distributor Label the outside of the box with CAUTION SENSITIVE ELECTRONIC INSTRUMENT Please enclose a note which contains the following a Sender
20. e LIES Insert Del Save Pgm Enter Note The loop has been deleted together with steps three 5 2 Insert program steps The programming spreadsheet Temp C Time lt Options gt L 99 0 Oh 5m Os 2 99 0 Oh 1m Os 33 55 0 Oh Im Os 2 24 4 34 0 Oh 1m Os MEO Insert Del Save Pgm Enter To insert a program step press Insert Del Insert Delete step 99 0 Oh 5m Os 2 99 0 Oh 1m Os 33 55 0 Oh Im Os 2 24 4 34 0 Oh Im Os lt Del Cancel Delete Insert Enter the number of the step that you want to insert delete Example To Insert new step at step 3 Enter 3 Insert Delete step 3 1 99 0 Oh 5m Os 23 99 0 Oh im Os 3 55 0 Oh Im Os 2 24 4 34 0 Oh Im Os lt Del Cancel Delete Insert Press Insert Insert Delete step 3 99 0 Oh 5m Os 2 99 0 Oh 1m Os Si 5550 Oh Im Os 2 24 4 34 0 Oh 1m Os gt gt gt Inserting new step at step 3 lt lt lt Whatman T3000 Manual 19 A new step is inserted Temp 0 C Time Oh Om Is Note The new step has been entered within an existing loop this loop now consists of three steps Temp C Time Options gt l 99 0 Oh 5m Os 22 99 0 Oh 1m Os 3 0 0 Oh Om is 4 34 0 Oh Im Os 2 24 Info Insert Del Save Pgm Enter Enter temperature and time for new step Note Additional steps will be inserted before the selected step The fo
21. e T3000 Thermocycler front view ccccccccssscessceseceseeeseeeseesssecsseeesecsseeseeeeeeseneeeeseeeseeeas 4 3 2 The T3000 Thermocycler rear view unesssseesseessennsnennnennnennnnennnennnennnnnnennnenennesnennn anne 5 3 3 The T3000 control panel eeeeeeeeesssessnensneesennnennnennnennnennnennnnensnennnennnnennnenssennsnensnensnnnnnn 6 34 Tmtalself ll ee ee 7 35 ThET3000 displayarza cases ia E E EEE AEE ia 7 3 6 Navigation within the T3000 software ccccccssessecssesssecssscssseesecseccseceeeeeeeseneeeeseeeseeees 7 3 7 Handling of the adjustable heated Lid cc ccsccsseceseceseeeseeceeceseesseeeseesseeesseseeeeseesenees 8 3 8 Releasing wheel in case of blocked lid oo ecccesecesecesceeseeeeeeeeeseseeeseesseessseesseeseeseees 8 4 Create PRO STAM sescscssccassessessieessoccnsversessiesensacusvonsesessdecsecctedeceanssonsdesadecesennssdcnsvonsesnbesacsesasiens 10 4 1 Select directory soere ieee EE uk aaediteg EEE ie 10 4 2 Select procram store GR EREE 11 4 3 Enter program name tens nnenaninnertiuneinenten 11 AA Enter lid tempersture n esueseesknin eisen nk 12 4 5 Select deselect lid pre heating u0usesseesseessenneensennennnennnennnennnnnnennne essen 13 4 6 Enter temperature and time settings eeeeeeneeesenesnennnennnnennnennnennnnnnnnnnnnnnnnnensnennne essen 13 4 7 SELIOOP uie ii elle HR nie ine il alleine 14 4 8 Cool below ambient temperature uueeessensseessnennennnennnennnennennnnnnnnennnen
22. ed and found to be defective This warranty does not apply to wear from normal use failure to follow operating instructions negligence or to parts altered or abused Whatman 36 14 Equipment Decontamination Certificate To enable us to comply with German law i e 28 StrlSchV 17 GefStoffV and 19 ChemG and to avoid exposure to hazardous materials during handling or repair will you please complete this form prior to the equipment leaving your laboratory COMPANY INSTITUTE ADDRESS TEL NO FAX NO E MAIL EQUIPMENT Model Serial No If on loan evaluationStart Date Finish Date Hazardous materials used with this equipment Has the equipment been cleaned and decontaminated YES NO delete Method of cleaning Decontamination NAME POSITION HEAD OF DIV DEP INSTITUTE COMPANY SIGNED DATE PLEASE RETURN THIS FORM TO BIOMETRA GmbH OR YOUR LOCAL BIOMETRA DISTRIBUTOR TOGETHER WITH THE EQUIPMENT PLEASE ATTACH THIS CERTIFICATE OUTSIDE THE PACKAGING INSTRUMENTS WITHOUT THIS CERTIFICATE ATTACHED WILL BE RETURNED TO SENDER Whatman T3000 Manual 37 15 Declaration of conformity EU Konformitatserklarung EU Declaration of Conformity G ttingen Mai 2004 im Sinne der EG Richtlinie tiber elektrische Betriebsmittel zur Verwendung innerhalb bestimmter Spannungsgrenzen 73 23 EWG following the EC directive about electrical equipment for use within c
23. ertain limits of voltage 73 23 EEC und and im Sinne der EG Richtlinie f r die elektromagnetische Vertr glichkeit 89 336 EWG following the EC directive about the electromagnetic compatibility 89 336 EEC Hiermit erkl ren wir da folgende Thermocycler Herewith we declare that the following thermocyclers Typen T3000 Thermocycler 20 T3000 Thermocycler 48 T3000 Thermocycler combi types T3000 Thermocycler 20 T3000 Thermocycler 48 T3000 Thermocycler combi Best Nr Order No 050 720 050 723 050 724 den grundlegenden Anforderungen der corresponds to the basic requirements of EG Niederspannungsrichtlinie 73 23 EWG und der EC low voltage directive 73 23 EEC and the EG Richtlinie ber die elektromagnetische Vertr glichkeit 89 336 EWG entsprechen EC directive about the electromagnetic compatibility 89 336 EEC Folgende harmonisierte Normen wurden angewandt The following harmonized standards have been used EN 55011 EN 55022 EN 61000 3 2 EN 61000 3 3 EN 61000 4 2 EN 61000 4 3 EN 61000 4 4 EN 61000 4 5 S EN 61000 4 6 EN 61000 4 8 2 EN 61000 4 11 5 Whatman
24. here is a convention on how time settings are entered in all BIOMETRA cyclers hours minutes seconds If you enter a number without dot this value will be interpreted as seconds 300 gt 5 minutes To program minutes enter a after the number of minutes To enter hours enter after the number You can also enter any combination of hours minutes and seconds Example for 1 hour 30 minutes 20 seconds enter 1 30 20 The time values will be displayed in the following format 0h 00 m 00s Enter 5 dot for 5 minutes initial denaturation Press Enter Temp C Time Options gt T 99 0 Oh 5m Os 23 3 4 Info Insert Del Save Pgm Enter Repeat for all temperature steps in the program 4 7 Set loop Note In general loops are defined by selecting the target for the back loop and the number of backloops Temp C Time Options gt 1 99 0 Oh 5m Os 23 99 20 Oh 1m Os 33 59 0 Oh 1m Os 4 34 0 Oh 1m Os Info Insert Del Save Pgm Enter In the row labelled with enter the target step for a backloop and press Enter Whatman T3000 Manual 15 Temp C Time lt Options gt I 99 0 Oh 5m Os 28 99 0 Oh 1m Os 3 55 0 Oh Im Os 2 4 34 0 Oh 1m Os nE Insert Del Save Pgm Enter In the row labelled with you can enter the number of backloops Note total cycler number n backloops 1 e g enter 29 for a t
25. ilable in three different block versions for 0 2m tubes 0 5ml tubes or both in the combi block 1 2 Easy programming The T3000 Thermocycler offers intuitive programming and a new memory structure New programs are easily created using the spread sheet layout that shows all important parameters on one screen Four softkeys directly below the display offer quick access to all functions needed in the individual context For easy retrieval programs can be stored in individual subdirectories Software options include both incremental and decremental temperature and time automatic restart after power failure and extended incubation at sub ambient temperatures 1 3 Higher speed The T3000 Thermocycler has been equipped with the latest in Peltier technology In addition the heat sink and ventilation system have been completely revised Thanks to these improvements the T3000 achieves faster heating and cooling performance High ramping rates provide both short experimental times and increased specificity 1 4 Smart lid technology Like all Biometra Thermocyclers the T3000 provides heated lids with automatic pressure control The temperature can be set for each lid individually between 30 and 110 C The unique design ensures the tubes are not damaged in any way by allowing the heated lid to be lowered until the optimum pressure is achieved This enables close contact between lid and tubes and reliably prevents excessive pressure Whatman 2 2
26. llowing steps are shifted by one position Note The insertion and deletion of steps may have an influence on existing loops Be sure that all settings are updated to maintain a correct cycle 5 3 Copy program Select the program you want to copy as described in section 0 and 4 2 21 di Sssecseeee ees B 0 TESTI B 1 TEST2 Edit program B 2 BIOMET B 3 BIOMET2 B 4 BIOMET3 Page N Page Vv Copy Del Edit Press Copy Del I 3 3 BIOMET2 Copy Pgm Delete Pgm Back To copy this program press Copy Pgm 0 Target 1 Cee 13 77 4 Info Page NV Back Enter Whatman 20 Select the subdirectory with the cursor keys or enter number on the keypad Co Target 511 Te 73 I 4 Info Page NV Back Enter Press Enter I 2 B 0 empty B 1 empty Target B 2 empty B 3 empty B 4 empty Page N Page Vv Back Edit Select program number for the program copy and confirm with Enter Copy e 3 3 BIOMET2 To ee Seer esas 3 empty Back Copy Press Copy Copy 3 5 3 BIOMET2 To ee en 3 3 empty
27. nnnnnsnnnsnnnnenssnensen 15 4 9 Save prO ptici roroi i ieie ein ka cn Ra lee in 15 gt Edit PKOSa Ms sissaccsasiccassecssoaisnssvenssonscsanesaisesecsavocssaneseaccoasasucssedseecdesosssansanasdedscamsssaasennseenssaonss 17 3 1 Delete pro erai Steps neck niet EEEE EEEa 17 3 2 Insert Prosfam steps ceseceessh exes be Sosdees sak A EEE EE ETE ET EIEEE 18 5 3 Copy PLOSlaM eeecccescccessceesseceeesecceneeessecceseeceesecseeeecseeseceeseseecesaeeseeeeeseeeseaueceeeeeesaeeees 19 54 Delete programcion deceads tots Eea ea a ia EEEn E ERATE E EEr E 20 6 Further programming options sessseseessescsssessoescesoeseessessosscesseseessosoooscescesoessosoosecessesoeseesoe 22 6 1 Program time increments ccccecsseessesesseessessseeseesseeeseeeseeceseeesecseesesseeeseeeseseseeeeeesesenees 22 6 2 Program touch down uesesssessneesnennsennnennnennnnnnnnennnennnnnnnnennnnnnennsnnnsnnnnnnensennsnnesnensennnnnnn 23 6 3 Adjust heating and cooling ramps ueeeeeeesseessesneennnnnnennnennnnennannnnnnnnennnennsennsnensnenneennen 23 7 Ruiprosram eessseenine in eseop ouuu sovas Soobe u Kue oS iSS OMU uE sEV Sai OSU Kss s ssoi 24 7 1 Select and start program ee ee 24 1 2 View program prior to Start ciinei eroras ane eere anhlnaiaa 25 7 3 Display during Operation ccccccecsscessseesseessessseeeecsseceecessecesecceeceeceneeeeseceseeeseeesseeeseeenees 26 7 4 View remaining run time cee eeccessessseesceesceesseeeeesseessese
28. ory this key moves to cursor forward to the next lower level The left cursor key moves the cursor back to the previous field lt gt In most screens this cursor is equivalent to the back softkey b gt In the file directory this key moves the cursor back to the higher level 3 7 Handling of the adjustable heated Lid To achieve optimum pressure on the tubes the T3000 is equipped with an height adjustable heated lid Close the lid After the samples have been placed in the block close the lid Turn the wheel clockwise until you hear a clicking noise In this mode the pressure will not increase further even when you keep on turning the wheel Note The pressure of the lid has been optimise for a fully loaded block If only very few tubes are loaded to the block you should place dummy tubes in the four corner positions to avoid damage of tubes by excessive pressure Open the heated lid First Release pressure by turning the wheel counter clockwise As soon as there is no more resistance the pressure has been released Then now you can open the lid with the knob Important The lid should not be opened under pressure because this leads to damage of the locking mechanism 3 8 Releasing wheel in case of blocked lid Note When the lid is in the extreme up or down position it may happen that the wheel is uncoupled In this situation the clutch mechanism is active in both directions clicking noise in either direction
29. otal number of 30 cycles Temp C Time lt Options gt 1 99 0 Oh 5m Os 2 99 0 Oh 1m Os 3 55 0 Oh Im Os 2 24 4 34 0 Oh 1m Os TARG Insert Del Save Pgm Enter This protocol now contains 25 cycles between step 2 99 0 C for Iminute and step 3 55 0 C for minute 4 8 Cool below ambient temperature Temp C Time lt Options gt 2 99 0 Oh 1m Os 3 55 20 Oh Im Os 2 24 4 34 0 Oh 1m Os 53 4 0 Pause TATO Insert Del Save Pgm Enter To hold the temperature for an indefinite time enter 0 After pressing Enter pause is displayed 4 9 Save program Save program with Save Pgm The program data are written to the permanent memory Temp C Time E Options gt 2 99 0 Oh 1m Os 3 55 0 Oh Im Os 2 24 4 34 0 Oh 1m Os 5 4 0 Pause TMEO Insert Del Save Pgm Enter Save program with Save Pgm Whatman 16 a mb E nn B 3 BIOMETRA Save changes Cancel Yes No Confirm changes with Yes The run time is calculated Sif esses B 3 BIOMETRA Number of steps 5 Run time 1h13m46 gt gt gt Saving program lt lt lt Note The run time is calculated according to the block type Please be aware that different block types provide different ramping rates see section 10 Technical specification Prog Step Temp 22 10 Z200 22 1C Time Lid 22 0C 22
30. r is NOT used to control the instrument Whatman T3000 Manual 31 9 Maintenance 9 1 Cleaning The T3000 was built to operate for a long time without the need for periodical maintenance The Thermocycler housing may be cleaned from time to time with a smooth cotton cloth Do not use strong detergents or organic solvents for cleaning 9 2 Trouble shooting 9 2 1 Slow heating and cooling The T3000 is equipped with a strong ventilator for the cooling of the heat sink The inlet of this fan is located at the bottom side of the instrument Be sure that the inlet is not clogged by dust or other material e g a sheet of paper placed under the cycler can be attached to the inlet as the fan is in operation Dust can be removed easily from the inlet with a conventional vacuum cleaner 9 2 2 Restart due to unrecognised power failure High voltage fluctuation can lead to an automatic restart of the thermocycler In this case the cycler restarts at the step where there power failure has occurred To avoid voltage fluctuation do not connect the cycler to a socket shared by a strong power consumer like a refrigerator or a centrifuge 9 2 3 Adaptation of protocols from other cyclers Since the T3000 is a fast instrument it may be necessary to reduce the heating and cooling ramps to run protocols from other cyclers For the setting of the heating and cooling ramps see section 6 3 Alternatively the time settings may be extended 9 3 Service an
31. s name and address b Name ofa contact person for further inquiries with telephone number c Description of the fault which also reveals during which procedures the fault occurred if possible Whatman T3000 Manual 33 11 Technical specification T3000 Thermocycler Order number 050 720 050 723 050 724 T3000 Thermocylcer 20 T3000 Thermocylcer 48 12999 o Capacity 3 blocks for 20 x 0 5ml 3 blocks for 48 x 0 2ml 3 combi blocks for 18 x tubes each tubes 0 5ml tubes or 48 well microplates or 48 x 0 2ml tubes or 6x er strips each or 48 well microplates or 6 x 8er strips Heating rate 2 1 C sec 2 2 C sec 1 4 C sec Cooling rate 1 7 C sec 2 0 C sec 1 2 C sec 0 5 C Temperature Uniformity Temperature range 3 C to 99 9 C Control accuracy 0 1 C Blocks Aluminium Program steps are easily entered in a spread sheet Software Options time increment temperature increment set ramping rate direct mode for use as thermoblock program storage in 10 individual subdirectories Program memory 10 individual subdirectories total capacity of 1 500 program steps equivalent to 250 average programs High brightness CFL backlight graphical LC Display Display viewing area 124 mm x 34 mm resolution 256 x 64 dots Auto restart after power Yes failure Cool samples at 4 C Yes Smart lid technology Yes Lid temperature range 30 110 C Power
32. secssecseeceeseeseeseeeesescneseeseeeseseaees 26 7 Pauses Stop Program aA aed Rie ee Rk 26 S System Settings vise ciniiscssccentsccisunsscsisesasanecanseuchsssinesdusccssevedesussagasesntesedsscasadasesavedesssasssnasesustanats 28 8 1 System Info 2a le R II REEL RR 28 8 2 Switch beep om OFF u a dal Banken 28 8 3 Selectlangu se assssieieiie ke aide antennae 29 Whatman Index 1 8 4 Display contrastans e E E R EEE EA E E IA 29 8 97 DIETOS To AEAEE EEE INEEN EAS E E E AN EE 29 8 6 Temperature readout ccccccccccsscsssceesecseeceseceseeeseeeeeeeseccseecsaecsseceseesscesseeeseeseseeeeessaeeses 30 9 Maintenant 550 sascsadsecacoessnsasasasssdassecsasacessedecassoassanessuasacesedsssanssennddenapaedseoadsaessunapsasesnecsdnaeees 31 9 1 Clean sauna een Ren 31 9 2 Troubleshooting aengeiketienlanemaalkkan 31 9 2 1 Slow heating and cooling eeesesssersseessensensnennnnnnnnennnennnnnnnnnnnnennnennsnnnsennsnnnsenennnn 31 9 2 2 Restart due to unrecognised power failure useesessesseensnenenennnnennnennnennnennnenn 31 9 2 3 Adaptation of protocols from other cyclers eeseeseesseesseesseeseeenennneeennnnnennnennn 31 9 3 Service And Tepalta neunten ennnaeen nikiesenneheitirepensschehieitenee 31 10 Instructions for return shipment eu scssosssosssssonsenssensonnssssonssnsssnsnnnunnsonssnnnsnsnnnnnnsonnssnnssssnne 32 11 Technical specification T3000 Thermocycler 00ssrs00us000002000000000n0008
33. tive inactive S earie Start Start LALO Direct Back Enter Select block with cursor keys gt and Block 1 Block 2 Block 3 Inactive Inactive inactive Start Start Start TATO Direct Back Enter Press Enter Start block 2 l Ae U N FO Page TV Direct Back Enter Select directory with cursor keys M Wor enter directory number and press Enter Whatman 25 T3000 Manual 3 Dieses B 0 BIOMETI DB 1 BIOMET Edit program B 2 BIOMET B 3 BERND2 EB 4 BIOMETI Page N Page Vv View Start Select program with cursor keys or enter program number Note To be sure that the correct program is selected it can be displayed by pressing View see section 7 2 Press Start to run program 7 2 View program prior to start Edit program Page M Page Vv u D 3 gt REN e oO w o w w w J O z H View Stresit To check a program prior to start it can be displayed by pressing View Page V Lid 3 BERND2 temp Preheating Back 109 0C Off Steuart Scroll through the program with page T and page 4 Temp C Time 1 99 0 Oh 5m Os 23 99 0 Oh 1m Os 3 55 0 Oh im Os 4 34 0 Oh 1m Os page TV lt
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