G-STORM GS1 - G-Storm Thermal Cycler Systems
Contents
1. e After performing the gradient experiment have a look at the gel image and select the best performing column e Double click the Gradient Step command to open its dialogue window e Select the Convert Gradient Step to a standard temperature step checkbox e Select the appropriate column from the drop down list in the Conversion section The thermal cycler software will now convert the gradient step into a temperature step and automatically select the right temperature in the chosen column NOTE If it is required to repeat the gradient experiment the converted temperature step can be set back to a gradient command just by unchecking the Conversion option 4 4 Deleting Programs rogram To delete an existing program click on the Home Page Depending on your user level guest or registered user the specific user directory or guest directory is displayed listing all existing program files Select the program you wish to remove G Storm Thermal Cyclers Programming 33 Click Delete This will remove the program from the script folder 4 5 Incubate temperature To run the cycler for incubations or ligations utilise the Incubate temperature function Click e Select the temperature that you require e Select the time you require OR e Select infinity Cycler will run until you Stop the program 34 Run Operation G Storm Thermal Cyclers 5 Run Operation 5 1 User Login 5 1 1 Guest Users When powering up2. escape of sample material 8 Installation G Storm Thermal Cyclers 2 2 Unpacking the Thermal Cycler 2 2 1 Package Contents The following parts are included in delivery Thermal Cycler User Manual on CD or memory stick G STORM GS1 Thermal Cycler X Power Cable 110 or 220V X Stylus x3 X G storm Ltd X 2 2 2 Initial Inspection All parts of the instrument were thoroughly inspected and tested before the unit was shipped therefore the instrument should be in good operating order Carefully inspect the instrument and its accessories for any physical damage sustained in transit If the instrument is received in a damaged condition please file a claim with the transport carrier immediately and contact G Storm advice G Storm Thermal Cyclers 9 2 2 3 Thermal Block The thermal block is shipped in separate packaging from the unit It should be removed from its packaging and located into position in the instrument Ensure that you push down the front and the rear of the block so that the block is fully engaged in its correct position Figure 2 1 G Storm GS1 cycler thermal block installation 10 Installation G Storm Thermal Cyclers 2 3 Functional Overview G Storm Ltd thermal cyclers are automated instruments designed for carrying out methods for amplification of DNA templates Lid pressure dial E Touch screen display USB Interface for memory stick o
3. selecting items and drag and drop 16 Software Operation G Storm Thermal Cyclers 3 3 Main Screen Upon power up of the system the screen first displays a start up routine followed by the Main Screen Home Page 7 G STORM AW USER Guest La Wizard Figure 3 1 G STORM GS1 Main Screen with navigation elements Service and Support Fewlecit Program Command ee Buttons 3 3 1 Command Buttons The Home Page Window comprises command buttons which provide quick access to the functions that are available on the GS1 Service and Support displays contact details for service and application Serve and Suppor support Login opens the Login dialogue window a gt d Run Program opens file list enabling users to select program to run ki A qa New Program opens the Compose Edit a Program dialogue G Storm Thermal Cyclers Software Operation 17 upon Jonge View Edit Program opens the file list to browse programs Incubate opens the incubate temperature screen Program Wizard opens the Program Wizard dialogue where you can define specific information Reports opens the report browser where you can search for stored Reports reports Settings opens the User Settings dialogue window where you can configure user settings such as defining the default start screen and manage cycler or user data 3 3 2 Additional functions Opens the Profile Status screen for a p
4. displayed Block not correctly inserted in the machine Check that the block is inserted correctly and reboot the machine Poor experimental yield 1 Run Thermal Engine OQ test to check that thermal engine operation is within specification 2 If transferring protocol from a different make of thermal cycler use TAS system to determine new protocol parameters Program does not start Block not correctly inserted in the machine Check that the block is inserted correctly and reboot the machine Tubes are crushed Applied pressure too high Condensation in tubes during arun Condensation will appear naturally at the end of a run and has no deleterious effects Simply spin or flick the liquid back to the bottom of the tube and continue with the post reaction steps However condensation during a run will result in sub optimal cycling If this is experienced try increasing the lid temperature Sealed microplate losing sample volume Poor quality microplate sealing system or inadequate pressure USB devices including memory stock and barcode scanner not recognized Reboot machine Machine does not power up and power light is not illuminated Check power is on at supply Check fuses at rear of instrument above power inlet Blank Display lit but showing no information Reboot machine If the problem persists there is an internal connection problem call Technical Support
5. Abnormal block heating cooling performance Run Thermal Engine OQ test to check performance G Storm Thermal Cyclers Trouble Shooting 45 1 Please refer to the thermal cycler IQ OQ Protocol document for instructions on performing instrument qualification tests 2 The Cycler Validation System CVS can be purchased separately from G Storm 46 Run Operation G Storm Thermal Cyclers 9 Block Exchange Block exchange can be performed within seconds No tools are reguired for this purpose CAUTION N Turn power off and interrupt power line before changing the block A Removing the Block Remove the block by simply holding it with two hands on the left and right side and lift it up Figure 9 1 Exchanging the block Inserting the Block Hold the block with two hands on the left and right side with the block connector at the back of the block and place it into position in the the thermal cycler G Storm Thermal Cyclers Block Exchange 47 Appendix Instrument Specifications Thermal Blocks Block Materials Traceability Blocks Available Modular anodised aluminium blocks with 4 sensors NIST traceable temperature calibration 96 well gradient block For 96 well plates or 96x 0 2 ml tubes 96 well gradient combi block For 96 well plates 96 x 0 2 ml tubes Or48x0 5 ml tubes 384 well high throughput block for 384 well plates 48 well block for 48 x 0 2
6. G Storm Thermal Cyclers
7. GEREEN SERS EER N EER Re GEED ESE ie 19 3 42 1 _ User Levels is ER et SE EE a DE Ge 19 Se New US ER EG a De Se EE 20 3 4 2 3 Logging in as Administrator 20 3 4 2 4 Adding or Modifying User Accounts 21 3 4 2 5 Deleting User Accounts etes ese es ee Ge ee AG ER ee ee dk dee 21 3 4 3 seel ERA OO N OR ER 22 de Lee Un e D 23 GreadlingProdrams ER GE Ge Ge ee he N Ee 23 4 1 1 DND Programming sie eis es se es DE N ie Go re thie 23 4 11 Program SiG Psi RE ENE EE ag Anh Rh De fede des 24 4 1 2 Program Wizard isi SEE steers BERE oe ENG EE Re ae ee Re GE SEEP De 30 General Programming Guidelines iese ee Re ee ee ee RR Re ee ee ee ee 31 Editing Programs ereire Ds see Deed de rn ede Ee ee ge ge 32 4 3 1 Editing Program Parameters iese sesse ee ee ee ee se se ee ee ee ee 32 4 3 2 Deleting a Program Step seek KERE Ee EER Ge KERS Ge ke ede 33 4 3 3 Converting a Gradient Step ss EE se ge Re dek See 33 Deleting Programs EE ace N beau eee eee a Ee ee ARE 33 Incubate temperature sensia antea eee ee ee ee ee ee 34 Run Operation vivecescaceiesveceacescicedcacerescetescnserenosenasveesressusesenssvensevetenssenesee 35 e EE 35 5 1 1 Guest EE 35 5 1 2 Registered Uleerg ii odes she rece GERRIE Re ESE N EEN Ne Gee ek eie ed 35 Changing Users ui EE ER REEN cats SEER GER ER GENEREER een ENG EER E Re eee 35 G Storm Thermal Cyclers 5 3 Viewing Script Temperature Profiles Prior ioun 36 5 4 Opening and Closing the Lid iss se e
8. a program when using a hot start polymerase Select the denaturation temperature and time When running the program the next command will be executed immediately after the denaturation time is elapsed Please also refer to the polymerase manufacturer s recommendations Manual Hot Start Manual Hot Start this step should be incorporated into a program when using a standard polymerase Select the denaturation temperature and time When running the program the instrument will notify you after the denaturation time has elapsed You will then be asked to open the lid and add the enzyme The instrument timer is now on hold and will continue only after you have pressed OK in the displayed Hot Start information window Eie e Temperature Step Use this command to select the required temperature in C and to define the step duration Processing Temperature Set the required temperature NOTE Temperature steps within a cycle must be greater than 30 C Hold for Set the required time in minutes and seconds If the hold at this temperature is option is selected the cycler will remain at the temperature 26 Run Operation G Storm Thermal Cyclers until the program is resumed This allows the user to determine when to continue to the next step Temperature Ramp Rate of change of the sample temperature Rates can be from 0 1 C to 3 C per second Unit is defaulted to run at maximum ramp rate Temperature Incremen
9. and maintenance of cyclers Archiving maintenance of all reports 3 4 2 2 New Users Only the administrator is authorised to set up new users and to change or assign user rights and passwords As a first time user you will be logged in as Guest user visible in the centre of the Home Page Guest users usually have restricted user rights and work from the unprotected Guest folder which can be accessed by all users Before you can operate the thermal cycler as a regular user your administrator has to set you up as a new user Once you are a registered user you have a personal encrypted file folder where you can administer your own program files 3 4 2 3 Logging in as Administrator After starting the thermal cycler the Main Screen appears displaying the thermal cycler s navigation elements 1 Click on the Login button on the screen 2 Select Administrator from the Username drop down list and enter the administrator password The default administrator password is admin 3 Click Login to confirm your settings 4 You are now logged in as Administrator visible in the centre of the Home Page screen IMPORTANT It is recommended that the factory set login password be changed to prevent unauthorised access to the instrument 20 Run Operation G Storm Thermal Cyclers 3 4 2 4 Adding or Modifying User Accounts NOTE Only the administrator can set up new users or modify existing user accounts To add a new use
10. 8 Blau Ee 48 G Storm Thermal Cycler Heated Did ER EE Seed eda AA Deg ER AR 48 BEA EE RE RR N 49 User amp File Management ee RE ee ee ee ee ee Ee Re ee ee ke ek 49 Reports and Validation ee ee ee ee ee ee ee 49 Barcode Option Si RE EG e Ee eek ES E SLEE Se ee Se SEE AA Ge EE 50 Thermal Engine CharacteristicS sesse ee Ge EE Re ee ee ee AE Ge ee ee ee ee 50 Power amp Dimensions ee ee ee ee ee ee ee ee ee ee ee ee 50 Compatible Barcodes iese ee ee ER EE Ee a 50 Compatible Plate Formats iese ee ek AAR AAR AAR RR Ee RR Ee RR ee Re ee ee RR ee Re 52 Order lnformatiO si ER EE SE GE EE Re ER GE Re el Med Ge Ge ee Ge DE we cod ve Ai 54 ee 55 nde sa RE ED DR cutee ED ER RA DA Ee RA tas 56 G Storm Thermal Cyclers General Information Policy Statement It is the policy of G Storm to improve products as new technigues and components become available G Storm reserves the right to change specifications at any time Warranty Statement G Storm Ltd guarantees that the thermal cycler you have received has been thoroughly tested and meets its published specification This guarantee is valid for 24 months only if the product and functions have been used according to the instruction manual No liability is accepted for loss or damage arising from the incorrect use of the thermal cycler G Storms liability is limited to the repair or replacement of the unit or refund of the purchase price at
11. G SIO RM G STORM GS1 Instruction Manual G Storm 2011 Release Information This document is protected by copyright All Rights Reserved G Storm Somerton Biotechnology Centre Ricksey Lane Catcombe Somerton Somerset TA11 7JH England Phone 44 0 7919 121510 Email info gstormdirect com Web www gstormdirect com Manual Version 1 0 Trademark Information e All product and brand names used in this document are trademarks or registered trademarks of their respective holders Gradient Capability is not available in The USA Germany or Japan Copyright 2011 G Storm G Storm Thermal Cyclers Contents General InformatiON Es RE REEN REEN KERE VS Ge RE GEE we ee Ke Ee Ge NE Dae Ek GEKEER EKS GE AE GE EE Ee 1 Policy EI ER N OR TT EE Ea 1 Warranty Statement ss ED dies dees ss dese Oe ee Ee 1 1 Safety Warnings and Precautions iese RE RR RR RR RR RR RR EE RR Ee RR ER RR 3 1 1 Before Operating the Instrument 3 1 1 1 Un D ln e Safety cis ces oi SR ts Ee ARE ERGER RE guia ER Ee RS ee 4 1 1 2 Symbols and Conventions iese ee see eed Re Ke Gee ee Ke 4 1 1 3 Potential Safety Hazards i ies esse ss ss se ee ee ee ee de ee ee sd ee ed ee ee 5 n SD ee TRV AM ens Ge oer be Ee ie Reg ee gee er de es er bee Ee 5 1 1 3 2 EXPLOSIVE SUBSTANCES AAA 5 EE ER D RR EE ME ON A ON 6 1 1 3 4 DANGER OF BURNS iss ER see See eek KS se Seg teas ese See eek Ese 6 1 1 3 5 OPERATING ENVIRONMENT sees sede e
12. G Storm s option G Storm is not liable for any conseguential damages G Storm thermal cyclers are for research use only Read the Instruction Manual carefully before using the thermal cycler to ensure that you obtain the best possible results from the machine G Storm thermal cyclers should only be used by suitably qualified and trained people If the thermal cycler is not used as specified in this manual the protection provided by the equipment may be impaired G Storm Thermal Cyclers General Information 1 G Storm Thermal Cyclers 1 Safety Warnings and Precautions 1 1 Before Operating the Instrument Ensure that anyone involved with the operation of the instrument is instructed in both general safety practices for laboratories and specific safety practices for the instrument Always place the instrument in a location where if necessary the main power supply can be disconnected immediately G Storm Thermal Cyclers Safety Warnings and Precautions 3 1 1 1 Instrument Safety Please read this section before operating the thermal cycler Operators of this instrument must be trained in both general laboratory safety practices and the specific safety requirements of the thermal cycler If the equipment is used in a manner not specified by the manufacturer the protection provided by the equipment may be impaired All functions performed within the context of preparing performing and completing a run should be don
13. ate 6500 Corning 384 Well Polyethylene Microplate 6502 G Storm Thermal Cyclers Appendix 53 Order Information Cat No Product Description Thermal Cycler GS00001 G STORM GS1 Thermal Cycler Thermal Blocks GSB00096 96 well block GSB09660 Combi block 96 60 well GSB00384 384 well block 54 Appendix G Storm Thermal Cyclers Sales amp Service G Storm Ltd Somerton Biotechnology Centre Ricksey Lane Catcombe Somerton Somerset TA11 7JH Phone 44 0 7919 121510 E Mail info gstormdirect com Visit our website www gstormdirect com 55 Appendix G Storm Thermal Cyclers Index AC Power Connection 15 Administrator Login 20 Automatic Hot Start command 26 Barcode Input command 25 Block Exchange 47 Changing Users 35 Cleaning 43 Command buttons 17 Additional functions 18 G Storm Logo 18 Incubate 18 Lightning flash 18 Login 17 New Program 17 Program Wizard 18 Reports 18 Run Program 17 Service and Support 17 Settings 18 User 18 View Edit Program 18 Compose Edit a Program 23 Creating Programs 23 Cycler Management 19 Cycler Settings 22 Cycler tests 13 Default Password 20 Deleting a Program Step 33 Deleting Programs 33 DND Programming 23 Editing Programs 32 End Cycle command 29 Exporting Program Files 41 Functional Overview 11 Fuses 15 Gradient Step command 28 Gradient Step Conversion 33 Ground
14. data click on Generate Program The Compose Edit a program dialogue window opens where you can view and edit the program steps To edit a program step double click the respective command To view a graphical representation of the defined temperature profile click Profile 30 Run Operation G Storm Thermal Cyclers 4 2 General Programming Guidelines To ensure a correct run the following guidelines should be followed when composing a new program e A program must contain at least one Temperature Step e Cycles cannot be nested A started cycle must have an End Cycle command before starting the next Start Cycle command e Barcode Input commands cannot be programmed inside a cycle e Only a single Script Information command is valid within a program The following commands or command combinations are considered stages e Automatic Hot Start e Manual Hot Start e A string of up to ten single Temperature steps e Single Gradient steps e A complete cycle including Temperature or Gradient steps within a start cycle end cycle pair e A Touchdown command e A Store command When using these stages in a script the following rules apply e The maximum number of stages within a script must not exceed 10 e The maximum number of Temperature and Gradient commands inside a cycle must not exceed 10 e The maximum number of cycles within a stage is 99 G Storm Thermal Cyclers Programming 31 4 3 Editing Program
15. e SEE Re Ee Ee de Re sed 36 33 Loading the Cycler Ms EA ee oe es ee DE oe eet 36 5 5 1 Loading ad AA ER N 36 5 5 2 Loading Kl 36 5 6 Setting the Lid Pressure se Mia EE ee Ee EE Se ee DBE oe 37 5 7 Program Exec tioN EE 37 5 7 1 Starting Aa RE EE N 37 5 7 2 Viewing Current Program Step Information ee 37 5 7 3 Terminating Ad RUIE ER ES SE ES EE Eer ER SE 38 5 8 EE 39 5 8 1 Viewing Report EE 39 9 9 Language Selection is EERS SEENEN dene GES Re ERG HERE OS 40 5 10 Switching Off the Instrument see es Reede ee EE Ee Re ee ee Ke gede 40 6 USB Memory Stick Operation RE EER RR RR RR RE EE RARR RR RR RR ER ER Rea Ee 41 6 1 Importing and Exporting Files ie EERS RS PRE GR ER ER Ee GR Ee ee ge ee neces 41 6 1 1 Exporting Program Files to a memory STICK cece ees 41 6 1 2 Importing Program Files from a memory stick to the GS 1 41 6 1 3 Uploading Primer Sequences from a memory stick 42 7 MAGA CG ss viri anis Ke Mee Es ke ee n Ge Gee ee ie ee ed 43 Re ee EE OE EE RE hate Aaa ta 43 8 Trouble Shooting is SA REK SE ee ei ei DER Re ee Ee ER og ee bi ER 44 8 1 Ifa Power Falure OU is EERS sk ge ee ar ER se Se Dae obec eN Ee 44 8 2 Troubleshooting Information Re ee ee ee ee ee 45 9 Block Exchange iss de se ER es N dd Ge de we ee eg ee ae ee gn dd 47 ReEMmOvinG Kee AT Inserting the BlOCK SA ER ED N Ed AT elen Le EG 48 Instrument Specifications ss RR EE cece GE Ee GE Ee GR ee ee ee ee ee ee ee ee ee ee ee 4
16. e with caution and care and with general respect both to the instrumentation and to associated chemicals samples and other devices 1 1 2 Symbols and Conventions The following chart is an illustrated glossary of the symbols that are used in this manual bd CAUTION This symbol indicates a potential risk and alerts you to proceed with caution A CAUTION This symbol indicates the presence of high voltage and warns the user to proceed with caution AN CAUTION This symbol indicates risks associated with hot surfaces 4 Safety Warnings and Precautions G storm Thermal Cyclers 1 1 3 Potential Safety Hazards 1 1 3 1 ELECTRICAL Standard electrical safety precautions should be applied e Ensure that the proper voltage is supplied before turning the instrument on for the first time e The device must be connected to a grounded socket e Do not touch any switches or outlets with wet hands e Switch the instrument off before disconnecting the AC power cord e Unplug the instrument prior to cleaning up any major liquid spills and prior to servicing any of the electrical or internal components Only qualified personnel should perform electrical servicing 1 1 3 2 EXPLOSIVE SUBSTANCES Explosive flammable and reactive substances should never be cycled or incubated in any thermal cycler Do not operate the thermal cycler in a hazardous or potentially explosive environment You must observe the releva
17. e your file within Windows naming conventions 4 1 1 1 Program Steps The following program steps are available in the Command List for creating customised programs in the Program Window 24 Run Operation G Storm Thermal Cyclers MP 123 Use this command to define barcode settings When composing the program Barcode Input it is recommended to place the barcode command at the beginning During program run you will be asked to read the plate s barcode You may do this with a handheld USB barcode scanner or by manually entering the barcode If you use the barcode scanner the barcode cannot be edited after reading The program will continue after you have placed the plate into the cycler and pressed OK The barcode will be recorded in the GLP and LabBook report and displayed in the Info section of the Cycler screen Scan Using Barcode Entry Barcode reading using the barcode scanner Scan Using Keyboard Entry Manual entry of the barcode via keyboard Path for worklist Specifies the path for the worklist Heated Lid Use this command to set the required lid temperature Lid Temperature The temperature may range from 112 C to 120 C The minimum increment is 1 C Lid is ON Select this option to activate lid heating NOTE The heated lid automatically switches off at temperatures below 25 C G Storm Thermal Cyclers Programming 25 O Automatic Hot Start Automatic Hot Start This step should be incorporated into
18. een In addition detailed program and instrument information can be viewed using the Program or Info buttons located in the right upper corner of the information screen Click Program to display the program steps of the running protocol The executed step is highlighted Click Info to view detailed instrument information such as temperature status lid status remaining time and step time or GLP file name G Storm Thermal Cyclers Run Operation 37 During a run it is possible to return to the Home Page by clicking on the Home button To return to the Profile Screen click on the G Storm logo in the centre of the Home Page 5 7 3 Terminating a Run To stop a program manually before it completes click the Stop button on the Profile Screen Alternatively use the Stop Program button on the Home Page changes from Run Program during a run The manual cancel of the program will be recorded in the GLP and lab book reports 38 Run Operation G Storm Thermal Cyclers 5 8 Reports 5 8 1 Viewing Reports Depending on the assigned user level you are allowed to view and archive LabBook reports stored in the following file folders User rights File folder Action Guest users Guest folder Viewing and archiving Lab Book reports The access to GLP reports is denied Registered users Guest folder amp Viewing and archiving Lab Book Personal file reports folder The access to GLP reports is denied Administrator All guest a
19. ells GSR0385 GS RIGID 384 black frame white cells G Seals GS 0500 Adhesive Clear Seal GS 0521 Peelable Foil Heat Seal GS 0536 Piercable Foil Heat Seal GS0541 Clear Heat Seal GS 0550 Adhesive Foil Seal 52 Appendix G Storm Thermal Cyclers The following plate formats are compatible with G Storm thermal cyclers Manufacturer Cat No amp Product Name Type BioPlastics B70511 96 Well thin wall plate 96 well non skirted B70661 96 Well thin wall plate 96 well semi skirted B70521 384 Well thin wall plate 384 well skirted Abgene AB 800 96 well skirted AB 900 96 well semi skirted AB 1111 384 well TF 0384 384 well Bilatec THERMOSPRINT 96 PP transparent 96 well non skirted 311296 only w o workstation THERMOSPRINT 384 PP transparent 384 well 310384 NUNC Art No 240600 96 well non skirted 96 Art No 230013 96 well semi skirted 96 Art No 230012 96 well skirted 96 Art No 230584 384 well 384 Eppendorf Art No 0030 128 648 twin tec plate 96 skirted Art No 0030 128 575 twin tec plate 96 semi skirted Art No 0030 128 508 twin tec plate 384 Corning Thermowell 96 Well Polypropylene Microplate Natural 6551 Thermowell 96 Well Polycarbonate Microplate Model M 6511 Corning 384 Well Polyproplyene Micropl
20. fter resumption of power if set to do so in the Cycler Settings The autopower fail restart function restarts the script from the beginning of the temperature step in which it failed The following table lists the three power failure scenarios and the actions the instrument takes if the power is interrupted during operation Observation Explanation Remedy Actions The computer fails but the In this case the thermal engine will continue to thermal cycler unit still has execute the script without any interruption power The thermal cycler fails but The execution of the script will be affected and will the computer still has power halt whilst the power is off Once the power resumes restart the unit and the script will resume from the beginning of the step it was executing when the power failed Both the computer and the The execution of the script will be affected and will thermal cycler unit lose halt whilst the power is off Once the power resumes power the script will resume from the beginning of the step it was executing when the power failed Corrective action Restart the application and the connection will be restored 44 Trouble Shooting G Storm Thermal Cyclers 8 2 Troubleshooting Information Refer to the following table for a description of potential problems and recommended actions that you should take Observation Explanation Remedy Actions No actual temperature
21. hermal cycler device It also deletes all scripts and GLP reports associated with that user NOTE The Remove User command also deletes all scripts and GLP reports associated with that user The Administrator user account cannot be deleted 3 4 3 Cycler Settings Browsing this option allows an Administrator to edit date and time settings and select the language for the instrument The Power Resume On Off option determines whether a program should continue or not after a power failure 22 Run Operation G Storm Thermal Cyclers 4 Programming 4 1 Creating Programs G STORM and GSPC software offers two innovative ways of composing programs Drop and Drag Programming DND Programming and the Program Wizard 4 1 1 DND Programming Drag and Drop programming DND programming is an easy and intuitive method for conveniently creating programs step by step The basic idea of DND programming is that you choose the required command from a command list drag it into the program window and drop it into the desired program position This opens a dialogue window where you can define further command specific settings such as time temperature lid pressure etc In this way you can create your program according to the planned programming sequence The command order can be changed easily by dragging the selected command to the desired position within the program window Once the program is started the cycler will process the commands from top
22. ing Requirements 15 G Storm Logo button 18 Hardware Installation 13 Heated Lid command 25 Home Page 12 Importing and Exporting Files 41 Importing Program Files 41 Incubate Incubate temperature 34 Incubate button 18 incubation 34 Initial Inspection 9 Installation 7 Block Requirements 8 Electrical Requirements 7 Requirements 7 Site Requirements 7 Thermal Block 10 Language Selection 40 Lid 11 Lid opening closing 36 Lid pressure 37 Ligation reactions 34 Lightning flash 18 Loading the Cycler 36 Login button 17 Main Screen 12 17 Maintenance 43 Manual Hot Start command 26 New Program button 17 New Users 20 Operating Power Requirements 15 Operation 35 Changing Users 35 Lid opening closing 36 Lid pressure 37 Running a Program 37 56 Appendix G Storm Thermal Cyclers Sample Loading 36 Terminating a Run 38 User Login 35 Viewing Program Profile 37 Viewing Script Temperature Profiles Prior to Run 36 Optional Devices 13 Order Information 54 Package Contents 9 Password 20 Program Execution 37 Program Steps 24 Automatic Hot Start 26 Barcode Input 25 End Cycle 29 Gradient Step 28 Heated Lid 25 Manual Hotstart 26 Script Information 29 Start Cycle 29 Store 29 Temperature Step 26 Touchdown 27 Program Wizard 30 Program Wizard button 18 Programming 23 Converting a Gradient Step 33 Deleting a Program Step 33 Deleting Programs 33 Editing Programs 32 Gene
23. is test Cycler Test procedures include specific instrument tests to ensure correct installation and operation of the thermal cycler After the tests have been completed successfully the instrument is installed operational and ready for use 2 4 2 Connecting Optional Devices Optional input devices such as keyboard mouse printer and barcode reader can be connected to the the thermal cycler via a USB connection e Plug a USB cable into the USB port of the input device G Storm Thermal Cyclers Installation 13 e Connect the other end of the USB cable to o the USB port of the thermal cycler OR o a USB hub if you wish to connect several input devices to a G STORM GS1 e When connecting several devices plug the cable connector of the USB hub into the USB port of the thermal cycler e For further configuration of the device consult the manufacturer s instruction manual 14 Installation G Storm Thermal Cyclers 2 5 AC Power Connection 2 5 1 Operating Power Requirements All G Storm Ltd thermal cycler models have self regulating power See Instrument Specifications Power amp Dimensions for power specifications 2 5 2 Fuses WARNING Disconnect the AC power cord before removing or installing a fuse to avoid the possibility of serious injury from electrical shock Fuse compartments are located on the rear of the instrument above the main power connection Check the voltage rating label to verify that the instrumen
24. ml tubes Heated Lid Lid Temperature Range Ambient temperature Relative Humidity 112 C 120 C user selectable in 1 C increments 10 C to 30 C 0 to 95 48 Appendix G Storm Thermal Cyclers User Interfaces Screen Type 6 4 inch TFT colour touch screen stylus supplied Touchscreen external keyboard System Input Optional amp mouse USB Optional barcode scanner USB Optional Real time graphical display of actual amp Temperature Display set block temperatures 1x USB side personal memory stick Lx keyboard barcode reader mouse Communication Interfaces USB hub User amp File Management User Level Management 3 levels Administrator User with administrator selectable restricted rights Guest User File Protection Yes for programs and reports File Organization Windows Explorer user defined folders amp subfolders Program Storage Minimum 99 internal memory or USB stick Circa 500 Unlimited PC program storage Reports and Validation Report Function Encrypted GLP report LabBook report Validation Ee l Internal auto validation prior to each program start Thermal engine validation function G Storm Thermal Cyclers Appendix 49 Barcode Option Barcode Option Barcode documentation via handheld barcode scanner Thermal Engine Characteristics Temperature Control Sample Vol
25. nd Germany Minimum Temperature Gradient To set the minimum temperature of the gradient step Min temperature must not be below 30 C Maximum Temperature Gradient To set the maximum temperature of the gradient step Max temperature must not exceed 80 C NOTE The maximum minimum gradient span is 30 C 4 C Hold for Duration of the Gradient step Convert Gradient Step to a standard temperature step The Gradient step can be converted easily to a temperature step after the annealing temperature optimisation experiment To do so just check the checkbox and select the temperature column with the best result of your optimisation experiment The converted gradient step will then automatically use the temperature of this best performing column NOTE Resulting temperature steps may be reconverted to gradient steps any time by disabling the Conversion option 28 Run Operation G Storm Thermal Cyclers amp i Start Cycle Starts a cycle Cycle Name Enter the name of the cycle Number of cycles Enter the required number of cycles tie End Cycle Insert this command to terminate a cycle NOTE All programs with a Start Cycle must have an End Cycle J Cools the thermal cycler down to a temperature between 4 12 C for a Store specified period of time Store Temperature Storage temperature in C Hold for Duration of the Store step Infinite Holds the specified temperature for an unlimited period
26. nd Viewing and archiving Lab Book user folders reports Access to GLP reports permitted To view reports proceed as follows Click Reports in the home page This will open the Browse Generated Reports window listing the generated reports Group the displayed reports by selecting one of the sorting criteria below Group by Date Sorts records in ascending order by date Group by Cycler Sorts records by cycler name Group by Program Sorts records by program name For viewing GLP and or LabBook reports select one of the following options Show GLP Displays the generated GLP records only for users with administrator rights Show LabBooks Displays the generated LabBooks 39 Run Operation G Storm Thermal Cyclers 5 9 Language Selection G Storm thermal cyclers can be operated in English Default and French To change the default langauge e Login as an administrator e Click Settings in the Main Screen e Click Browse on Cycler Settings e Select the language you require e Click OK The unit will then remain in the selected language 5 10 Switching Off the Instrument The unit can be switched off at any time during operation using the power button at the side of the instrument There is no need to exit the thermal cycler software prior to switching off the instrument 40 Run Operation G Storm Thermal Cyclers 6 USB Memory Stick Operation 6 1 Importing and Exporting Files You can import Primer seguences and p
27. nt safety regulations when handling pathogenic material radioactive substances or other substances hazardous to health G Storm Thermal Cyclers Safety Warnings and Precautions 5 1 1 3 3 FLUIDS Reaction vessels should be filled outside the cycler so that no fluids penetrate the instrument Do not submerge the instrument in any liguid 1 1 3 4 DANGER OF BURNS Thermal block inner side of heated lid and reaction vessels quickly A attain temperatures of greater than 50 C Do not touch Risk of Burns Keep the heated lid closed until temperatures of 30 C or lower are reached Do not use any materials plates sealings foils mats which are not sufficiently temperature stable up to 120 C 1 1 3 5 OPERATING ENVIRONMENT Please ensure that the ventilation slots of the device remain free to vent at all times A space of at least 10cm should be left around the thermal cycler The ambient temperature should be between 10 C and 30 C the humidity between 0 and 95 WARNING G Storm s service engineers must carry out all repair work Only original G Storm replacement parts must be used Disconnect the instrument from the mains supply before opening 6 Safety Warnings and Precautions G Storm Thermal Cyclers 2 Installation 2 1 Requirements The following steps must be followed for optimal and safe operation of the thermal cycler 2 1 1 Site The thermal cycler should be placed on a rigid fla
28. of time NOTE When executing the Store command the lid pressure is set to zero Newton G Storm Thermal Cyclers Programming 29 Script Information Use this command to record your comments about the program 4 1 2 Program Wizard The Program Wizard TV is a unigue feature that makes it easy for first time users to write their own protocol The Program Wizard utilises biological data to generate a program Enter primer and template information e g Primer seguence or melting temperature template length and origin of DNA The Program Wizard is also an ideal tool for first shot reactions as it uses proven calculations e g for the Annealing Temperature To generate a new program click on Program Wizard in the Main Screen 1 Primer Information Enter either primer sequences or melting temperatures Sequences can be uploaded from a txt file on a memory stick Click Browse in the Sequence Primer One Sequence Primer Two section and browse for the required files in the Upload Sequence from USB Memory Stick dialogue window Select the desired file and click Open to upload the file to the thermal cycler 2 Product Length Enter the length of your product either manually or using the slide bar 3 DNA Source Information Select from Bacterial Eukaryotic or Plasmid 4 Specials Select any special requirements that your program may require e g type of enzyme high AT GC content 5 After you have supplied all required
29. r click New User to open the Add a new User dialogue Assign a user name and password Select the required user level Guest User Administrator from the drop down list Now you can define the user s script reading and writing privileges by checking the appropriate boxes Edit Rename Script The user has the right to edit or rename a program file Delete Script The user has the right to delete program files Create Script The user has the right to create program files Once you have entered all data click on Create User to save the settings After successful setup a confirmation message will be displayed and the new user name will be added in the User Management list To modify an existing user highlight the user name and click Edit User to open the Edit a User dialogue Here you can modify user data such as name user level password user privileges and script directory protection Click OK to save the changes A confirmation message will be displayed after successful update of the user data 3 4 2 5 Deleting User Accounts NOTE Only the administrator can delete User accounts The User Management dialogue lists the available user names and corresponding user levels Select the user name you wish to remove G Storm Thermal Cyclers Software Operation 21 Click on Remove User to remove the user name from the user list It removes all user permissions that the user has and does not allow that user to access any the t
30. r keyboard Power switch Figure 2 2 G Storm GS1 thermal cycler profile view The G STORM GS1 has a manually operated lid The dial on the top of the lid should be turned to the fully closed position to apply adequate pressure to the sample vessels The G STORM GS1 is operated via its own internal PC and a universal software application offering independent control of the thermal block G Storm Thermal Cyclers Installation 11 Service and Support View Edit Program N USER Guest Figure 2 3 G Storm GS1 thermal cycler Main Screen Home Page 12 Installation G Storm Thermal Cyclers 2 4 Hardware Installation 2 4 1 Starting up the G STORM Once you have set up the G STORM as explained in section 2 1 and 2 2 you can connect it to the mains e Plug the AC power cord into the main power connector at the rear of the instrument and connect the other end of the cable into a grounded AC outlet e Adjust the LCD display to an acceptable viewing position e Press the power switch on the side of the instrument e The cooling fan powers up and the thermal cycler will go through a short initialisation routine Then the Home Page appears providing access to the thermal cycler s navigation elements e Perform Cycler Tests o Login as an administrator o Click Settings button o Click Browse cycler management o Click Cycler Tests o Click Engine Test Ensure a tube or plate is in the block for th
31. ral Guidelines 31 Program Wizard 30 Reports 39 Viewing Reports 39 Reports button 18 Run Program button 17 Running a Program Starting a Run 37 Safety Hazards 5 Instrument 4 Symbols and Conventions 4 Safety Warnings 3 Sales amp Service Centres 55 Script Information command 29 Service and Support button 17 Settings button 18 Software Operation 16 G STORM and GSPC Software 16 Required Computer Skills 16 Specifications 48 Compatible Barcodes 50 Compatible Plate Formats 52 Heated lid 48 Neworking amp Barcode Options 50 Power amp Dimensions 50 Reports and Validation 49 Thermal Blocks 48 Thermal Engine Characteristics 50 User amp File Management 49 User Interfaces 49 Start Cycle command 29 Starting a Run 37 Starting up the G STORM 13 Store command 29 Switching Off the Instrument 40 Symbols and Conventions 4 System Settings 19 Temperature Step command 26 Terminating a Run 38 Thermal Block 10 Touchdown command 27 Trouble Shooting If a Power Failure Occurs 44 Information 45 Trouble Shooting 44 Unpacking the Thermal Cycler 9 Uploading Primer Sequences 42 USB Memory Stick Operation 41 User Accounts Adding or Modifying Accounts 21 Deleting Accounts 21 G Storm Thermal Cyclers Appendix 57 User button 18 Registered Users 35 User Levels 19 User Management 19 User Login 35 View Edit Program button 18 Guest Users 35 Viewing Program Profile 37 D I Appendix
32. ram 5 4 Opening and Closing the Lid The lid can be opened and closed manually at any stage 5 5 Loading the Cycler WARNING AN Danger of Burns The thermal block test tubes and plates may reach temperatures as high as 100 C Before loading or unloading the cycler keep hands away until temperature reaches 30 C or less 5 5 1 Loading Plates Depending upon the thermal block installed the thermal cycler can be loaded with one 96 well plate or one 384 well plate Put your plate on the block and ensure that it is correctly positioned in the provided space 5 5 2 Loading Tubes G STORM thermal cyclers can be loaded with up to 96 tubes 0 2 ml or 48 tubes 0 5 ml when using the 96 well combi block 36 Run Operation G Storm Thermal Cyclers 5 6 Setting the Lid Pressure Close the lid and turn the knob until firm contact is made between the heated lid and tube or plate 5 7 Program Execution 5 7 1 Starting a Run To start a run click on the Home Page Your personal file folder will open or if you are logged in as a guest the guest folder opens displaying all available programs Select the program you wish to run Click Run selected on the right hand side of the window enter your sample volume 5 150ul and click OK to start the selected program 5 7 2 Viewing Current Program Step Information After a run has started a graphical representation of the temperature parameters is displayed on the Profile Scr
33. rogram which is running on the G STORM instrument Provides the operational status of the instrument flashes green when Bi the instrument is running User Provides information on current user 18 Run Operation G Storm Thermal Cyclers 3 4 System Settings Specific system settings can be defined by clicking on the Settings button when logged in as an Administrator 3 4 1 Cycler Management Browsing this option allows an Administrator to edit cycler settings and perform cycler validation tests 3 4 2 User management Browsing this option allows an Administrator to manage cycler users 3 4 2 1 User Levels Three different user levels are available e Administrator e Registered User e Guest User Each level has specific user rights Only administrators are authorised to set up new users and to change and or assign user rights and passwords By default the thermal cycler starts with Guest user rights User Rights Functions allowed Guest User e Access rights to the Guest folder e Creating copying editing and executing programs stored in the Guest folder Registered User e Access rights to the Guest and personal user folders e Creating copying editing and executing programs stored in the Guest and personal folders e Access to GLP reports denied 19 Run Operation G Storm Thermal Cyclers Administrator e Access rights to all Guest and user folders Setup and maintenance of users Setup
34. rograms created on a different thermal cycler or on a PC to the G STORM thermal cycler and GSPC software or export programs and reports that have been created using a USB memory stick 6 1 1 Exporting Program Files to a memory stick e Make sure the USB memory stick is inserted in the USB slot on the side of the instrument e Click View amp Edit Files to display your personal file folder Select the desired program e Click Save As to open the Save Program dialogue window e Enter the name of the program e Activate the Save to USB checkbox The file is then saved to the USB archive NOTE the lock button on the memory stick may prevent data access Please ensure the lock button is in the unlocked position 6 1 2 Importing Program Files from a memory stick to the GS1 e Make sure the USB memory stick is inserted in the USB slot on the side of the instrument e Click View amp Edit Files to display your personal file folder Click View USB to access the USB archive e Select the desired file from the archive e Click Save As to select a file name and path G Storm Thermal Cyclers USB Memory Stick Operation 41 e Confirm with OK to save the file 6 1 3 Uploading Primer Sequences from a memory stick The Program Wizard function offers the option to upload primer sequences stored in an external memory stick e Click the Program Wizard button e Click Browse in the Sequence Primer One Sequence Primer Two sec
35. s 4 3 1 Editing Program Parameters You can edit the parameters of existing programs and store the program with the same name or rename the edited program Fewrecit Program To edit an existing program click on the Home Page Depending on your user level guest or registered user the specific user directory or guest directory is displayed listing all existing program files Select the program you wish to edit e Click Open Opening the file automatically loads the program and the program steps are displayed in the Program Window of the Compose Edit a program screen e Double click the command you wish to edit This will open the dialogue window of the respective command e Edit the parameters as needed e Click OK to save your changes and to return to the Compose Edit a program screen e After you have modified all data click Save in the Compose Edit a program screen if you wish to store your changes in the existing program e Click Save As if you wish to rename the program You will then be prompted for a file name 32 Run Operation G Storm Thermal Cyclers 4 3 2 Deleting a Program Step To remove a program step from the program drag it from the Program Window and drop it into the recycle bin icon located below the Program Window Save the program as described above 4 3 3 Converting a Gradient Step A gradient step can be converted to a standard temperature step with a few mouse clicks
36. se ee nee Pe se Bee ee 6 2 EEUE d RR OE OE N N ES ia naire 7 ER Ee al el N EA OR OR EE EE SEN 7 2 1 1 EE EE AE ee 7 2 1 2 Seele EE 7 2 1 3 Thermal Eeler 8 2 2 Unpacking the Thermal Cycler iese esse ee Rea EE EE Ge Ee ee eke eo 9 2 2 1 Package Contents issie eege 9 2 2 2 Initial INSPECTION an AA ee ee ee ee 9 2 2 3 Thermal Block 10 2 3 Functional Overvlew EA AA AA Re AA Re AA Re AA AA Re AAR Re AA Re AA Re ee 11 2 4 Hardware Installation ee ees ese eke Re RA AA AA AA AA AA AA Re AA Re ee Re AA Re AA Re ee 13 2 4 1 Starting up the G STORM ergste se SE Se Gee ea Ee Ed 13 2 4 2 Connecting Optional Devices ee ee RR Re ee ee ee 13 2 5 AC Power ConnectiON ees ee ee Re AA AA AA AA AA Re AA AA Re ee Re AA Re ee Re ee 15 2 5 1 Operating Power Reguirements iss ek ER ER ER ER Re 15 2 5 2 BA EE RE 15 2 5 3 Grounding Reduirements ees sees sees see ee ee ee 15 G Storm Thermal Cyclers II 3 1 3 2 3 3 3 4 4 1 4 2 4 3 4 4 4 5 5 1 5 2 Software Operation sesse oe bees Ee ege be ed 16 G STORM and GSPC Software see esse dee ee de see ee RE dee kk SE Ee ee Re Ge ke 16 Required Computer Skills ee ee EE ee ee Ge ee ee ee Rd ee ek ee Eg ee 16 Main KEE 17 3 3 1 Command Buttons ES EE ER oe ae a We N Ee ee eg Gage 17 3 3 2 Additional functions EE EE EE N Vee ER EE 18 e Ee 19 3 4 1 Cycler Management veer ELE EERS AE ER ESSEN EG Re ER Rg GE Gee Ee de 19 3 4 2 User management sees SEE
37. t is compatible with the AC voltage available at the installation site 2 5 3 Grounding Requirements DANGER please read carefully To protect operating personnel the National Electrical Manufacturers Association NEMA recommends that the instrument be correctly grounded The instrument is equipped with a 3 conductor AC power cord that when connected to an appropriate AC power outlet grounds the instrument To preserve this protection feature do not operate the instrument from an AC power outlet that has no ground connection G Storm Thermal Cyclers Installation 15 3 Software Operation 3 1 G STORM and GSPC Software G STORM thermal cyclers are operated via their own internal PC running under Windows and a universal software engine offering simple and intuitive instrument control The unique Program Wizard function enables stepwise protocol definition with no tedious calculations required Drag and drop icon based commands make all steps quick clear and intuitive throughout Optimised conditions from gradient protocols are converted to standard protocols with only a single point and click The programming software can be installed additionally on any laptop or desktop PC running WIN 2000 or XP The memory stick supplied allows quick and easy data and program exchange between PC and the thermal cycler s internal PC 3 2 Required Computer Skills Users should have basic computing knowledge including using the mouse
38. t Temperature increment and decrement per cycle Steps can be from 0 1 C to 10 C NOTE For decrement use negative figures e g 2 Time Increment Time increment per cycle in seconds Step Type Indicates the selected step type for information purposes The supplied step type information will then be displayed in the cycler monitor LC Touchdown is an advanced technigue used to reduce non specific binding Touchdown With the G Storm thermal cycler Touchdown is provided as a one step command for easy implementation in the programming sequence Denaturation Temperature Defines denaturation temperature Maximum Annealing Temperature Sets the maximum annealing temperature Minimum Annealing Temperature Sets the minimum annealing temperature Hint The temperature difference is commonly between 5 C and 10 C starting with a temperature that is 2 C above the higher Primer melting temperature Example if Tm Primer 1 is 60 C and Tm Primer 2 is 54 C the resulting Touchdown will be from 62 C to 52 C G Storm Thermal Cyclers Programming 27 Elongation Temperature Sets the elongation temperature Cycles Number of required cycles for the Touchdown section Duration Duration of the temperature step in seconds Gradient Step Use this command to define settings for the gradient function Just enter the required minimum and maximum temperature gradient and time Gradient steps are not available in the USA Japan a
39. t clean surface Make sure that the instrument stands completely stable Adequate ventilation is important Make sure there is sufficient space so that the rear and side air slots are not covered and to allow cooling air to circulate freely around the instrument There should be no paper under the device as this may block the ventilation path The unit should always have at least 10cm distance to the next wall or neighboring instrument Gene Technologies Ltd thermal cycler instruments were developed for operation in laboratories in which there is normal ambient temperature and no explosive atmosphere The ambient temperature should be between 10 C and 30 C the humidity between 0 and 95 2 1 2 Electrical The thermal cycler must be connected to a grounded socket G Storm Thermal Cyclers Installation 7 2 1 3 Thermal Block e Reaction vessels should be filled outside the thermal block so that no fluids penetrate the instrument e To prevent damage to the block and the heated lid make sure you use only recommended sample tubes Unsuitable tubes can be damaged causing the escape of sample material This may present a serious health hazard especially when working with infectious materials e Make sure plates are positioned correctly in the block to prevent damage to the block and the lid e Ensure that the heated lid is closed when heating tubes in the block otherwise tubes may burst due to high temperatures causing the
40. the instrument the default user level is Guest If you wish to continue to operate the cycler with Guest user rights no special login procedure is required In this case you can access the unrestricted Guest folder and operate the cycler as described in the relevant chapters 5 1 2 Registered Users Once the administrator has set you up as a registered user see 3 4 2 4 you can operate the thermal cycler using your personal data and file folders To log into the system as a registered user proceed as follows e Click on Login the Login dialogue opens e Select your user name from the Username drop down list and enter your password e Click on the Login button This will log you into the system giving you reading and writing rights as specified for your user level e After successful login your user name is displayed in the top status bar NOTE In case a user forgets his or her password the logged on administrator can assign him her a new one 5 2 Changing Users In order to log into the system when another user is already logged in proceed as described in section Registered Users above G Storm Thermal Cyclers Run Operation 5 3 Viewing Script Temperature Profiles Prior to Run Before starting a run you can view the temperature profile of a new or stored program Click on Profile in the Compose Edit a program dialogue window An information screen is displayed detailing all the parameters of the selected prog
41. tion and browse for the required files in the Upload Sequence from USB Memory Stick dialogue window e Select the desired file and click Open to upload the file to the thermal cycler NOTE Primer Sequence files must have txt file format 42 Run Operation G Storm Thermal Cyclers 7 Maintenance 7 1 Cleaning G Storm Ltd thermal cyclers are designed to reduire a minimum amount of maintenance by the user They can be cleaned using water or a mild laboratory cleaning agent The instrument should not come into contact with organic solvents or aggressive solutions Ensure that no liguid enters the thermal cycler For safety reasons the device must be switched off and disconnected from the power supply prior to cleaning The electrical safety fuses are located above the main power switch and the main power plug at the rear of the device Before the fuses are replaced the device must be switched off and disconnected from the mains supply Only fuses with the correct voltage values may be used Only qualified service personnel should perform servicing of the thermal cycler No warranty obligation is assumed by G Storm Ltd in the event of damage caused by unauthorised servicing G Storm Thermal Cyclers Maintenance 43 8 Trouble Shooting 8 1 If a Power Failure Occurs All G Storm Ltd thermal cyclers are equipped with an auto power fail restart function that allows for power failures and safe continuation of a run a
42. to bottom according to the defined programming sequence To generate a new program using DND click on the Home Page This will open the Compose Edit a Program dialogue with its drag and drop functions G Storm Thermal Cyclers Programming 23 ComposelEdit a Program Command List Pa Close Lid Automatic Hot Start E 7 ns Hot Start ED Start Cycle Temperature Step l End Cycle Gradient Step Touchdown The Current Program is valid Open Lid Run Time 00 00 00 EER J store aD 3 HI Barcode Input H Save as Cancel D Script Information Figure 4 1 Compose Edit a program screen Plek KEE EE ZA The Compose Edit a program screen consists of the Command List which contains all available program steps and the Program Window where you can create or edit programs 1 Select a command from the Command List then drag and drop it into the Program Window This will open the dialogue window of the selected command 2 Enter your settings in the dialogue fields of the selected command 3 Confirm your settings by clicking OK This will return you to the Compose Edit a program window To add another command to your program repeat steps 1 3 for each required command Click on Profile if you wish to view a graphical representation of the defined temperature profile Once you have finished creating your program save it by clicking on Save As and you will be prompted for a file name Nam
43. ume Range Sample Accuracy Sample Homogeneity Heating Cooling Sample Overshoot Gradient Temperature Range Maximum minimum Gradient Span 4 C 99 C with simulated volume dependent control algorithm 5 150ul 0 4 C 20 99 C 1 C 4 20 C 0 4 C Up to 3 C per second Up to 3 C per second lt 1 C 30 C 80 C 30 C 4 C Power amp Dimensions Electronic Power Supply Dimensions LxWxH Weight Operating conditions Regulatory Warranty 100V to 240V frequency 48 to 62Hz 465mm x 337mm x 265mm 12kg approx 10 C 30 C 0 95 relative humidity CE UL CSA compliant 2 years on all systems Compatible Barcodes The following barcodes are compatible with G STORM s documentation system 50 Appendix G Storm Thermal Cyclers e 2of 5 interleaved e Codabar e Code 128 e Code 39 e Code 39 full ASCII e Code 93 full ASCII e EAN 13 EAN 13 with 2 supplement EAN 13 with 5 supplement e EAN 8 EAN 8 with 2 supplement EAN 8 with 5 supplement e EAN 128 e UPA UPA 13 with 2 supplement UPA 13 with 5 supplement e UPE UPE 13 with 2 supplement UPE 13 with 5 supplement e MSI e CIP G Storm Thermal Cyclers Appendix 51 Compatible Plate Formats Cat No Product description 96 plates GSRO960 C IGS RIGID 96 clear frame clear wells GSR0961 GS RIGID 96 black frame white cells GSR0384 C GS RIGID 384 clear frame clear w
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