CO2 Incubator User Manual
Contents
1. E Installation and Connection of Gas Source The correspondent connecting interface is at the back of equipment See picture 6 P10 The connecting pipe is included with the unit The gas entering the equipment should first pass a reduction valve to reduce the press to 1 bar The press of 1bar cannot be changed for safety reason E 002 Connect the reduction valve to the equipment again E Attention CO2 gas should be food class and its purity is at least 99 5 CO2 Cylinder with cat is not allowed The sensor with high sensitivity is used in this incubator and it cannot be used under electromagnet interference e g mobile phone E Put through the Main Power Please ensure the power supply matches the power written on the nameplate before putting through the power There is a grounded power cable together with the incubator The main power needs 2 4A fuses Instruction Manual Page 4 The surrounding temperature is at least 8 lower than the SET value X Keep the outer door and glass door open E Distilled water used X Pour the distilled water into the pool approach the room temperature Input Quantity 3L Avoid overflow during the inputting Ensure the humidity inside the chamber will not change X Open the cutoff valve of the bottle X Switch on the main power The green light is on 888 will be displayed for about 40s Regular Self checking Actual value will be displayed after Se2. Floor 3 4 th Building 1 No 201 Tianlin Rd Shanghai 200233 P R China
3. 12 KA is used to increase the value 13 CO gt 2 is used to set concentration of CO2 14 GAS light will lighten when the gas comes in 15 Cis used to set the temperature 16 Heating light will lighten in heating progress Instruction Manual Page 5 34 Control Panel Picture 2 HF240 Display Panel 1 2 G3 G O 7 c a C 8 16 8 5 wo V cal 2 14 auto start 90 C 13 door open over temp 1 1D 0 Simple Introduction of Display Panel 1 C Display Window Displays the actual value at normal condition The SET value can be displayed by pressing KC key The code of temperature malfunction can be displayed by pressing i key you can also switch to the special function mode by pressing cal key See Function Select P 19 N Heating light will lighten in heating progress 3 KC is used to set the temperature 4 KAJ is used to increase the value 5 KY is used to reduce the value 6 Press Ki key will display the malfunction code 7 Press auto start key for 10s to enter the Auto start mode Instruction Manual Page 6 34 Control Panel 8 Press cal key for over 5 seconds to enter the adjusting mode Select special functions together with KY and KA key 9 Press 90 C key for 10s to enter the disinfection at 90 C See P 22 10 Over temp will lighten if the temperature is over the set value and the heating progress will be terminated 1
4. A HEAL FORCE progress will be terminated Page 3 34 Simple Introduction of Display Panel 16 15 14 13 12 11 10 Press Kil key will display the malfunction code 1 C display window Displays the actual value at normal condition The SET value can be displayed by pressing KC key The code of temperature malfunction can be displayed by pressing Kil key you can also switch to the special function mode by pressing cal key See Function Select P 19 2 CO 2 display window Displays the actual value of CO concentration the SET value can be seen by pressing COz _ key The code of COs malfunction can be displayed by pressing Ki key you can also switch to the special function mode by pressing cal key See Function Select P 19 Press cal key to enter the adjusting mode Select special functions together with KY and KA key Auto start light will lighten when the equipment begins Auto starting Door open light will lighten when the glass door is opened 90 C light will lighten when the equipment begins disinfection at 90 C Over temp will lighten if the temperature is over the set value and the heating Instruction Manual Page 4 34 Control Panel 9 Press 90 C key for 10s to enter the disinfection at 90 C See P 22 10 is used to reduce the value 11 Press auto start key for 10s to enter the Auto start mode
5. for more than 5s Loose the cal key when CAL is glittering in the temperature display window Press COz2 once and the concentration will be displayed in COz2 window Adjust the concentration by pressing KAJ or KY according to the actual value Confirm by pressing KiJ key Repeat the steps above until the value is according with the precision Instruction Manual Page 27 34 HF90 Technical Data Chamber Shelf Number of Shelves Standard At most Width Depth Net Weight Electric Data Rated input voltage 230 Rated Power 0 65 Rated Power Frequency 50 60 FUSE AC250V F6 3 A Digital Temperature Control Range Power Cost at 50 C Chamber Deviation DIN 12880 Transient Deviation DIN 12880 Instruction Manual Page 28 34 Digital CO2 Control Set Range 0 20 CO gt 2 Set Precision 0 1 COr Recovery Time About 1 CO2 min Gas Source About 3 7 L min Humidity Relative Humidity Coat Texture Paint Data of Gas Source Filter Ratio Grain Strained Purity Ratio At Least Entrance Press Instruction Manual Page 29 34 HF 240 Technical Data Chamber Shelf Number of Shelves Standard At most Width Depth Net Weight Electric Data Rated input voltage Rated Power 0 735 Rated Power Frequency 50 60 FUSE AC250V F6 3 A Digital Temperature Control Range Power Cost at 50 C Chamber Deviation DIN 12880 Transient Deviation DIN 12880 Ins
6. Manual Page 15 34 Micro Control System E Door Heating On Off E Function 5 The main heating system allows the equipment working at the temperature 8C higher than the surrounding If you want to operate at the temperature 5 higher than the surrounding you can simply switch off the door heating system But condensed waiter will form on the glass door Default set Door heating system on E Heating Way Normally Slowly E Function 6 The two different heating ways ensure the equipment working normally at different temperature The Slowly Heating Way is suitable for high temperature situation Normally Heating Way is suggested Default Set Slowly Heating E Operational Way Default Set E Function 7 This Set id to test the function of the incubator please set to 1 Default Set 1 E Operational Way Monitoring the surrounding temperature E Function 8 Monitor the surrounding temperature to make the temperature inside the chamber steadier Default Set Monitoring the surrounding temperature Instruction Manual Page 16 34 Overheat Protection E Temperature Limit Controller TLC There is a separated TLC to protect the incubator When the temperature control circuit is out of control the TLC will control the power in case itis 1 5 C higher than the set value The red light over temp will be lit when the TLC responds If the TLC is charged with the controlling please refer to the code table of malfunction to fi
7. by KY and A key when keeping pressing the cal key C will display the Function code 1 and press cal again after loosen it CO display window will show the actual mode The mode can be changed by pressing 1 V CA Cc Display Window CO Display Window On Off On Off CO Zeroing KA 1 KA 0 KG 1 G o 5 Door Heating Switch 6 Heating Way the 8 Monitor Surrounding Temperature a S 0 KS 1 Kd 1 Ka O Kh 0 Kh 1 KP 1 KP 0 Please refer to Page13 Page14 for details Not Opened Opened On Off Slowly Normally Not Monitoring Monitoring Default Set Opened On Slowly Monitoring Instruction Manual Page __21 34 E Attention E Temperature Please restart AUTO START for calibration when setting temperature is over 1 C which make the unit works precisely ECO Comparison If the value of CO2 concentration measured is different from the value showed on the CO2 you can adjust CO zeroing by manual Unit will recover to the original CO2 zeroing after next AUTO START operation E Example for Reference COz display value 7 0 CO2 CO actual value 6 2 CO Choose function No 3 by Kcal key and KA key Loosenthe cal key XPress the cal key again The KC displays K 3 and glitters CO display window shows K 0 X Input the actual CO concentration value into the temperature display
8. heat disinfection Way of 90 C moist heat disinfection Open the outer door and glass door and take out all the materials inside the incubator Dry and clean the incubator and input 300ml distilled water Close the glass door and outer door Turn on the power supply Keep pressing the 90 C button on the display panel until the green light 90 C on the display panel lightens Wait for about 10s the C display window will show OPE and CO display window will show dor Please check id there is anything left inside the incubator Please do this after opening the outer door and glass door After keeping opening for one minute the CO window will show 25 and C window will show the actual value of temperature and glitter which indicates the disinfection procedure will last for 25 hours Please close the doors The equipment now begins the disinfection Instruction Manual Page 44 Disinfection Cleanness and Examine Explanation of 90 C moist heat disinfection A The whole procedure is divided into three phases Heating The heating procedure will increase the temperature to 90 C and the procedure will last for 2 hours based on surrounding temperature Sterilization The temperature has arrived 90 C and the sterilization will last for 9 hours Recovery The equipment will return to the incubation mode decrease the temperature and the procedure will
9. last for 14 hours Note The procedure of disinfection may last for longer time in the conditions where the heat is difficult to emit If the procedure is over 25 hours while the temperature has not recover to 37 C then CO will always display 4 4 hours remains At that time operator can terminate the disinfection manually by keeping pressing 90 C Jkey until the light goes out which will not affect the disinfection Input 3L distilled water and do SUTO START after disinfection Incubation can only be started after AUTO START The C window displays the actual value of temperature while the CO window has three displays show the remaining time and remind you the current phase Phase 1 the cursor of the COz window is climbing in E g 25 25 gt 25 Phase 2 the cursor of the CO window is at the middle and glittering in E g 23 gt 23 Phase 3 the cursor of the CO window is descending in E g 14 14 14 Instruction Manual Page 5 4 Disinfection Cleanness and Examine E Warning Please do the cleaning and disinfecting before informing the maintaining engineer E Maintenance We can assure the normal condition of the equipment only when it is maintained or improved by engineers authorized by Shanghai Lishen The equipment should be overhauled every a certain time to ensure the normal condition X Suggestio
10. the center of the incubator Wait for another 2 hours after the temperature value is steady and note the actual value Way of Temperature Adjusting m Open the outer door observe the temperature inside the chamber and note it down 8 Close the outer door and keep pressing the Kcal key for more than 5s 8 Loosenthe CAL key when the glittering cal appears on the display window 8 The CO2 window will display the temperature value if you press the KC key m Adjust the temperature according to the noted actual value by pressing KAJ or KY key 8 Confirm by pressing iJ key 8 Repeat the above steps until the precise temperature Condition of CO2 adjustment The temperature and humidity of incubator is steady and keep the situation for more than 2 hours The concentration of CO2 should be 0 COz zero adjusting 8 Press COz2 Y to set 600 to 0 Press cal key for more than 5s Loose the Kcal key when CAL is glittering in the temperature display window Press AUTO START once to make 0 0 appear in the window Loose AUTO START andpress iJ until the 0 0 is glittering in the window The procedure will be completed after two or three minutes Adjust the concentration of 2 The concentration should be higher than 2 usually is 5 7 m To set the concentration of CO2 5 0 Wait for more than 0 5 hour for stabilization then measure the real concentration Press Kcal
11. 1 90 C light will lighten when the equipment begins disinfection at 90 C 12 CO is used to set concentration of CO 13 Door open light will lighten when the glass door is opened 14 Auto start light will lighten when the equipment begins Auto starting 15 COz2 display window displays the actual value of CO concentration the SET value can be seen by pressing COz2 key The code of CO malfunction can be displayed by pressing Kil key you can also switch to the special function mode by pressing Kcal key See Function Select P 19 16 GAS light will lighten when the gas comes in Instruction Manual Page 74 Introduction HF90 amp HF240 Incubator is a delicate lab equipment mainly used in medical area and incubation of cells tissues and bacilli There are new functions like disinfection at 90 C the control of CO2 concentration and the Micro Control System which improve the incubation of cells tissues and other materials It is an important equipment of High Leveled biological and medical experiments Please carefully read this manual before using in order to prevent the inconvenience and the damage to the equipment Only the personnel authorized by Shanghai Lishen can do the necessary test or maintenance work in order to keep the incubator work normally and accord with the corresponding safety standards Please note the Serial No when handling the malfunction report or speak for compon
12. 34 Micro Control System Code Table of Malfunction Code of Malfunction Possible Reason Solutions 99 Glass door is open Glass door is not closed Close the door 100 Temperature too low Switch on the door heating Set point Door heating is switched off Function 5 101 Temperature too high Surrounding temperature Switch off the door heating Set point too high Function 5 CO not connected Connect the gas 7 as T CO gt is used up Replace the CO bottle p Low press of CO entrance Adjust the press to 1bar 201 COz too high Set point High press of COzentrance Adjust the press to 1bar d Special Function Adjusting Page 19 Choose the following functions by cal KY or KAJ E Buzzer On Off E Function 1 You will hear a sound signal when malfunction occurs if the buzzer is on Switch off the buzzer Silent Default set Buzzer is on E Gas supply On Off E Function 2 The gas source will be cut off if you choose Gas OFF andthe green diode Gas will crush out Default set Gas On the green diode Gas lights when the gas enters E CO zeroes E Function 3 If the warp of COz display is too large the function allows manual zeroing CO E Lock the SET point E Function 4 This function allows you lock the SET point of temperature and CO2 SET value and these points will not change when the equipment is wrongly operated Default set Unlocked Instruction
13. O source to 1bar 0 1MPa Press COz2 and KY J KA to input CO setting value eg 5 0 7 0 or 0 Unit will input the COz gas until the setting value Now the unit can be used in incubating work You must set the COz2value to K 0 according to the Step 10 after operating before switching off Open the glass window to spill out the gas and dry the inner chamber Instruction Manual Page 1 34 Control Panel 3 1910011616001 2anc icmecinncosndcladeSessciesedencasercsencapereonesacees 7 Application 8 Equipment Description 8 Structure Inner Component Heating System Humidity Gas source Door Switch Press Compensation Micro Control System 11 a Data Control lt sce lt o2ceace oa nce ee ace a 11 Temperature COz Control b Self adjusting of Measuring System 12 Regular AUTO START Function c Supervisor Function 12 Door Control Malfunction Identification Error Range Wrong Message Code Table of Malfunction d Special F nction soe er emg NR Rese Sa 13 Buzzer on off function 1 Gas Supply on off function 2 CO zeroes function 3 Lock
14. Only qualified personnel using suitable tools may work on supply lines and compressed gas container bottles or collective systems in which CO2 is stored for use with the incubator Warning Warning Important operating and or maintenance instructions Read the accompanying text carefully Caution hot surface Warning be careful of the glass Caution risk of electric shock CAUTION fuse N ak Protective GND indicator Simple Operational Procedure of HF90 8 HF240 CO Incubator Default set Temperature 37 07 CO2 0 Please perform 90 C moist heat disinfection before first using refer to P 22 Then the following procedures should be strictly followed 10 11 12 13 14 Open the outer door and the glass door and input distilled water 3L into the pool Close the doors after doing so Connect the unit to the source of COs Connect the power supply and switch on Self checking will be done automatically CO2 and KC willdisplay K888 and version number The checking will be terminated after 40s CO2 and C display the setting data inside the unit Press the auto start for about 10s then the auto start light will be lit Please open the glass door according to the hints of the CObz and KC display windows Close the glass door after 1 minute and the unit will do AUTO START Wait for 16 to 24 hours Adjust the exit pressure of C
15. af i Heal Force CO Incubator HF90 HF240 User Manual Version 2005 07 29C COPYRIGHT Without the written permission of our company any content of the manual should by no means be duplicated or disclosed to any other individual or organization Individual or organization should in no way use the manual unless authorized by our company Our company has the sole right to change or modify this manual Any other individual or organization thereby should in no case change or modify the manual Without our written permission any organization or individual should not by the name of sale or donation provide the public the manual or the duplication of the manual Anyone who violates our copyright must be pursued according to the copyright law Shanghai Lishen Scientific Equipment Co Ltd No part of this publication may be reproduced or transmitted in any form by any means including photocopying and recording without the written permission of Shanghai Lishen Various sections of this operating manual may be copied only for in house use by the equipment operator e g to provide employees with instruction on accident prevention measures These sections are clearly marked in the list of contents Shanghai Lishen can accept no liability or responsibility for the marketability or the suitability of his operating manual for a certain purpose other than that specified under Areas of application of equipment Shangha
16. alarm 2h when malfunction 4 Runoff the gas The max gas input through the capillary per hour when the valve is open Press of f Excretion of Gas Capillary Entrance 4 Requirement of Gas 1 Cost of Gas Excretion of Gas when 2 Malfunction 3 Type of Gas High point LSOD 09 Instruction Manual Page 34 34 Consume of CO door closed Dm Nu BMAD 1 6 CO concentration CO2 11 16 Picture 13 Consume of CO door closed PH value of incubation liquid The pH value is affected by the concentration of CO inside the incubator The following picture shows the concentration 21 relationship between pH value and COs Media PH Value CO2 16 14 12 10 Media NaHC03 g 1 DMEM BME MEM Media 199 Mc Coy F 10 F 12 MEM Hanks Salts 3 2 2 70 20 20 6 8 6 9 7 7 1 7 2 iv PE Ph Value 7 4 7 5 7 6 Picture 14 PH value concentration of CO inside the incubator Global Exclusive Agent Nison Instrument Shanghai Limited Address Floor 15th No 2 Hua Shan Road Shanghai 200040 P R China TEL 86 21 62728646 62712931 FAX 86 21 62710529 E mail export hf healoo com Website www healforce com Manufacturer Shanghai Lishen Scientific Equipment Co Ltd Address
17. ature lost when the front door is opened Way of teardown Refer to Figure 3 1 Open the small glass door at 45 and lift slowly until it is completely removded E Six small doors Option for HF240 There are six small doors located in the front of the inner chamber The unique design can reduce the gas and the temperature lost when the front door is opened Way of teardown Refer to Figure 3 2 Open the small glass door at 45 and lift slowly until it is completely removded Instruction Manual Page 9 34 Equipment Description Picture 4 Draining E Heating System The heating system is spreaded on the surface of inner chamber to heat it The heating system is normally placed at the top back front left right sides of the pool and the front of the outer door The condensed water will not appear because the outer door is also heated so that it will keep clear On the contrary there will be condensed water on the outer door The main heating system allows the equipment to run at the temperature 8 C above the Room temperature If you hope to operate at a lower temperature you can switch off the door heating to make the equipment work at the temperature 5 above the surrounding There will be condensed water formed at the corner of the glass door The door heating switch is ON at normal condition refer to function 5 P19 and the additional heating system is always on the new added slowly heating and normally heat
18. ents Only authorized or trained personnel can operate the equipment Please keep the incubator in steady environment without acute change of temperature which is helpful to the incubation The equipment quotes the following standards Q TEUCs 2002 HF90 Q TEUC4 2003 HF240 EN 61010 2 010 Equipment Model and its Meaning HF COz Incubator 90 Moist heat disinfection system at 90 C The inside volume is 151L 240 Moist heat disinfection system at 90 C The inside volume is 240L Sorts of equipment Common equipment of Class I The important part is labeled by IN Instruction Manual Page 4 The incubator can simulate the natural data of cells and tissues The equipment CANNOT be used to incubate flammable materials Equipment Description Picture 3 1 Picture 3 1 ThreeSmall doors Small doors E Fabric Shell Electrolyzed galvanization steel RAL 9002 Control parts are made of plastic Stainless steel is used in inner chamber E Inner Components The insert shelves are shake resisted and can be adjusted at 50mm height You can remove the shelves and support frame simultaneously without any additional tools Three small doors HF90 There are three small doors located in the front of the inner chamber The unique design can reduce the gas and the temper
19. i Lishen reserves the right to change the version of this operating manual at any time and without prior notice Environmental conditions Indoors use Altitude up to 2 000 m The work s Temperature surrounding must be in18 C 30 C when the unit work in 37 C Maximum relative humidity 80 for temperatures up to 30 C MAINS supply voltage fluctuations up to 10 amp of the nominal voltage Transient over voltages typically present on the MAINS supply category II Applicable RATED POLLUTION degree 2 The room must be equipped with adequate ventilation The setup surface must be firm level and nonflammable The unit must be set up where it will not be in direct sunlight There is not any hot source nearby the unit Safety Information It is important for you to read these Instruction Manual carefully before using the unit for the first time The COz Incubator may only be operated by trained authorized personnel Maintenance work on the unit may only be performed by the Shanghai Lishen or authorized agents Tissue materials or liquids Which are highly flammable or potentially explosive Whose vapors form flammable or explosive mixtures with air Which release toxins May not be used The pressure of the CO supply can be adjusted to a range of 0 8 1 bar and cannot be altered CO2 is a kind of gas be representing potential health hazard The unit is to be repeated at suitable intervals
20. ing mode could make the incubator work at the different temperature Anyway normal heating mode is suggested E Humidity The distilled water in the pool vaporize and humid the air inside the chamber and the humidity at normal condition is 295 The condensed water will not appear at the top of the chamber and on the door because the particular heating system is used Instead it will gather at the other sides of the inner chamber The container of the pool 3L distilled water Please ensure that there is no chemical material in the water or it will canker the pool Each incubator is equipped with an electric suction pump Steps of using X Take out the draining pipe in the bag X Remove the lower shelf inside the chamber X Connect one point of the pipe to the discharge Instruction Manual Page 10 34 Equipment Description Picture 5 Electric Suction Pump 1 Drainpipe 2 Bottom of pump 3 Osculum 4 Plug port of the electric suction pump X Put the other point into the bucket X Install the four osculums onto the back of chamber below the wind channel and put the bottom into the water X Educe the plug and put it into the 220V power jacket The pump begins to work until the c hamber is dried X Take off the plug and remove the pump from the chamber X Unplug the drainpipe and dry the pump X Dry the pool Attention of using the pump The pump cannot run without sopping up Do not make the
21. lf checking E Adjust the SET Point Keys used KC CV A X Keep pressing C keytoadjustthe KC value X Keep pressing CO2 key to adjustthe COz value Display the value being set last time The last number of the display glitters Set the expected value by KY or A key and the value will be stored after loosening the SET keys and the actual value will be again displayed E Default Set Temperature 6 CO 0 0 Attention Only air gas can be in the chamber AUTO START X Keep pressing the auto start on the control panel for 10s OPEN DOOR The temperature display panel will show opE CO display panel will show dor and open the outer door and glass door for about 60s The Auto start mode is activated and the CO display panel will show 0 0 and the C display panel will show the actual value after closing the doors GAS light is off and AUTO START light will be on X Close the doors Instruction Manual Page 204 E Attention Heat the equipment to the SET value and establish the relative humidity After regular AUTO START Auto start light will be off Actual value displayed The equipment will continuously input the gas until it reaches the SET value that has been set The equipment can now be used in incubating work E Special Functions cal and Y or KA keys You can choose and set the special function
22. mperature and humidity both reach steady state The process costs 15 hours if it is started at the room temperature Please ensure that the chamber is only filled with air Suggestions Do AUTO START every six weeks or whenever the temperature is changed c Supervisor Function Door Control All the actual values displayed will glitter until the glass door is closed The 5 minutes lasting of door open will be considered as malfunction and a malfunction code will be given out X Malfunction Identification Micro Control System supervises all the data and possible malfunctions All the SET value has a range and the equipment will give an alarm together with the wrong message if the actual value is out of the range X Error Range Temperature 0 5 C COs 1 0 CO gt r X Wrong Message Each malfunction identified by Micro Control System has a special code that will help you recognize the reason of malfunction The radiation digital tube will glitter when malfunction occurs to inform you the situation and if the buzzer is connected you will also hear sound signal If you keep pressing i key you will get the code of malfunction on the display and if there is no faults it will display X Attention Please press i key to get the code at first when malfunction occurs Following actions may interrupt wrong Message Change the SET value Switch on off the equipment Instruction Manual Page 14
23. n The equipment needs an annually check to keep working properly Each Incubator is strictly checked before being putting into use and it can suit your daily needs Please contact the engineer of Heal Force if you want to keep it at top precision X Condition for Normal Use A Ambient temperature 18 C 30 C B Relative humidity lt 80 C There is no violent shake or corrosive gas near the equipment D No effects from sunshine or other heat or cooling source X Shanghai Lishen reserves all the rights of updating and improving of the product Warranty The warranty period is one year after purchasing Our company will not take responsibilities if the malfunctions are caused by improper use even it is within the warranty period 6 Our company will responsible for the maintenance after the warranty period but a certain amount of cost will be provided Please show the related materials to the authorized personnel Protection Signals Lit Avoid shaking knocking and water during the transporting Condition of transportation and restoration Temperature 40 C 55 C humidity lt 95 Instruction Manual Page 26 34 Adjust the Data The equipment should be adjusted regularly to ensure the best working condition The user could adjust the equipment under the help of professional personnel at least once a year Condition of Temperature Adjusting Put a thermometer with the minimum scale as 0 1 C into
24. nd out the reason Instruction Manual Page __17 34 Placing Position and Installation Open the box and take out the components E Positioning The incubator must be put at the irremovable place Avoid direct irradiation from the sun The placing position should keep dry and the surrounding temperature cannot be higher than 30 C 18 C 30 C is the most suitable Keep the equipment at a level place and the bracket should be shake resistant and apyrous COzis needed when the incubator is working while CO is also harmful to health so the placing position must be good ventilation The gas discharged from the back panel must be immediately carried off The equipment cannot work without ventilation device thus if several equipments are in the same room or the equipment is placed at the bottom of the lab additional ventilation device is needed For the detail of gas releasing please refer to the appendix MSpace There should be some space left between the equipment and the wall or other instruments refer to picture 8 9 120 Picture 8 Space between the HF90 incubator and the wall mm Instruction Manual Page 18 34 Placing Position and Installation 4 SMART CELL E Picture 9 Space between the HF240 incubator and the wall mm The press compensation hole at the back panel cannot be jammed Stacking See picture 11 P30 picture 12 P31
25. pump run for more than 10 minutes Drainpipe must be connected wnen draining Dry the pump after draining x Don t take water to the plug of pump because of the AC 220V working voltage E Gas Source The joint of the gas entrance is at the back panel of the equipment refer to Picture 6 The max input press is 1 bar Please carefully check the joint of gas Ay entrance The gas will pass a filter and enter the chamber after the grain larger than 0 3um being sieved The sieving ratio is 99 998 and the blower mixes the inputting gas and air inside CO2 gas should be high pure food class gas Picture 6 CO2 entrance Door Switch There is a switch at the back of the glass door This switch will cut the gas supply and the heating system to prevent the inputting gas and overheating All the display will glitter until the door is closed If the door remains open for 5 minutes the equipment will give out an alarm The outer door can only be closed when the glass door is closed and gastight Instruction Manual Page 114 Equipment Description E Press Compensation The press compensation hole prevents the abnormal press when the gas enters and assimilating of air when glass door is open See Picture 7 The equipment should be placed with good compensation to ensure the gas letting by valve can be cleaned in time Picture 7 Pressure Compensation Hole Instruction Manual Page 12 34 Micro Con
26. s ole COs entrance press too low ee 23 144 PIES 201 CO too high SET p Adjust the entrance press point COz entrance press too high fo thar Please contact the maintaining engineer if the table above cannot solve the problem E Malfunction that cannot be shown by the equipment Way of Checking Equipment does not heat Check the SET value No gas entrance Check the diode GAS Check if the input is switched off Check the SET value Buzzer does not give out alarm when Check if the buzzer is switched on malfunction occurs Please check the special function SET value cannot be changed SET point is locked Please check the special function There is much water on the glass door Check if the door heating is switched on E Switch Off Switch off the main power Switch the gas reduction valve Instruction Manual Page 23 34 Disinfection Cleanness and Examine Need to Do Once a Week Warning Hot surface in Check the water level of the pool Disinfection mode Check if the entrance press is 1bar don t touch and open the door E Clean the Chamber with 90 C moist heat diinfection suggestion once per 2 months Switch off Open the glass door Dry the chamber 90 C moist heat disinfection The whole procedure of 90 C moist heat disinfection o i C Heating Sterilization i Recovery 2 l 9 14 Hours Picture 10 The procedure of the 90 C moist
27. the SET Point function 4 Door Heating normally slowly function 6 Monitoring the surrounding temperature function 8 Instruction Manual Page 2 34 Overheat Protection 15 Placing Position and Installation 16 Placing position Cleanliness Stacking Installation and Connection of Gas Source CO2 Attention Put through the Main Power Gas Analyzer 51 8018 2 i ee 18 Adjust the SET Point Default Set AUTO START Special Functions Attention i key Table of Malfunction Code 21 Disinfection Cleanness and Examine 22 90 C moist heat disinfection 22 Adjust the Data 25 Temperature Adjusting 25 CO2 AGUSUNG 2 2 8 esas oa 25 Technical Data 2 222 2222 22222 25sec e2ee5ci 4 2 2 enai 26 100616106 hh re a Na Sa to Pots 29 Stacking of Incubators Capacity of Gas CO Inputting PH value onon A Instruction Manual Control Panel Picture 1 HF90 Display Panel 8 A auto I start fet ore fv C auto start door open over temp
28. trol System The System includes the following separated control function a Data Control Set Range X Temperature 5 0 C 50 0 C XCO 0 0 20 0 b CO Zeroing Adjust the CO testing system c Supervisor X Door Control X Wrong Message X Malfunctions Restoration X Codes of Malfunction d Special Function x Buzzer On Off Gas Supply On Off CO Adjusting Set Point Lock Heating Mode Slowly Normally Door Heating On Off a Data Control E Temperature A Pt1000 resistance of Micro Control system controls the chamber temperature ECO Control In order to keep the stabilization of pH value of the samples the concentration of CO in the chamber should be controlled The concentration of CO2 depends on the expected pH value and the content of buffer solution of NaHCO in the incubation medium The measure of CO is a continuous process based on the thermal conductivity of inner chamber Thus the input of CO will cause the change of the thermal conductivity inside the chamber and form a signal indicates that there is a direct ratio between thermal conductivity and concentration Instruction Manual Page _ 34 Micro Control System b Self adjusting of Measuring System Regular AUTO START function The Measuring System needs adjusting before operating or modifying the SET temperature It will be preceded when AUTO STARTING The equipment will do self adjusting to the measuring system when the te
29. truction Manual Page 30 34 Digital CO2 Control Set Range 0 20 CO gt 2 Set Precision 0 1 CO gt 2 Recovery Time About 1 CO min Gas Source About 3 7 L min Humidity Relative Humidity Coat Texture Paint Data of Gas Source Filter Ratio Grain Strained Purity Ratio At Least Entrance Press Appendix The stacking of incubators refer to picture 11 12 Capacity of gas under normal and abnormal condition Appendix of CO aeration refer to picture 13 P 33 PH value based on the concentration of COs refer to picture 14 P 33 Instruction Manual Page _ 31 34 Stacking Picture of HF240 SMART CELL SMART CELL Picture 11 Stacking figure of HF240 Instruction Manual Page 32 34 Stacking Picture of HF90 Picture 12 Stacking Picture of HF90 Instruction Manual Page 33 34 Stacking of Incubators refer to Figure 11 12 Additional aeration is needed if there are several equipments in one room or the equipment is installed at the ground floor of the lab The two equipments can be stack together by the piling feet which are riveted on the top of the equipment E Capacity of gas under normal and abnormal condition 1 Gas required The necessary gas quantity for SET point 2 Gas cost The gas quantity at max SET point 3 Malfunction Gas output at the max SET point and in case of sound and light
30. window by Kcal CV KAN XLoosen the cal key XPress Ki to confirm CO will display the modified concentration If the actual value is higher than the SET one please keep the door open for one minute to let the extra gas out E Important X Please pour out the water in the pool and dry the chamber if the equipment is not to use for a period of time X Do not switch on off the equipment continuously X Please keep the door closed and try to shorten the opening time when necessary to ensure the best situation of the chamber E i key If any malfunction occurs during work time the corresponding display will glitter and if the buzzer is connected you will hear a sound alarm at the same time You can know the reason of malfunction by pressing i key and the display window will show the code of malfunction Refer to the table of malfunction code Page21 Instruction Manual Page 224 Table of Malfunction Code Please check the following to solve the problem more quickly Code of Malfunction Reason of Malfunction Solutions 99 Glass door is Open Glass door is not closed Close the door 100 Temperature too low Seals Switch on the door SET point Door heating is switched off heating Function 5 1 01 Temperature too high Surrounding temperature too Switch off the 7 SET point high heating Function 5 Connect the gas input COs gas not connected 200 CO too low SET point CO bottle is used off Replace ne CO
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