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Combidrop User Manual D01515 - QB3

1. Figure A 58 Columns and channels for a 48 well plate setup Table A 21 Channel and column settings for a 48 well plate Dispensing cassette Multidrop Combi Channel settings Column settings Channel Tube in tubing weight Column Selection A inplace 1 selected B inplace 2 unselected C removed 3 selected D inplace 4 selected E inplace 5 unselected F removed 6 selected G inplace 7 selected H inplace 8 unselected 9 selected 10 selected 11 unselected 12 selected Thermo Fisher Scientific Appendix B Certificate of Decontamination Name Address Tel Fax Name Serial no A I confirm that the returned items have not been contaminated by body fluids toxic carcinogenic or radioactive materials or any other hazardous materials B I confirm that the returned items have been decontaminated and can be handled without exposing the personnel to health hazards Materials used in the unit Chemicals Biological e Radioactive Specific information about contaminants Decontamination procedure Date and place Signature Name block capitals The signature of a Radiation Safety Officer is also required when the unit has been used with radioactive materials This unit is certified by the undersigned to be free of radioactive contamination Date and place Signature Name block capitals PHOTOCOPIABLE Please include decontaminating solution used Thermo Fisher Scientific Thermo Scien
2. 2 Make sure you are in the Main menu Select the plate type and insert the plate First select the cassette type and then the dispensing volume Keep the PRIME button down until the tubings are completely filled Press the START button to start the protocol The instrument dispenses the plate Press the EMPTY button to empty the dispensing tubing During prolonged standby keep the cassette in its rest position Remove the cassette when the maximum amount of plates are dispensed or you want to change the cassette type in use Make sure you wash the cassette properly ped OG COCOTTE f i a Power OFF the instrument 36 Thermo Scientific Multidrop Combi User Manual Thermo Fisher Scientific Routine Operation Three key After the instrument is turned on make the following selections to start selections to start dispensing when you are in the Main menu plate type dispensing volume and cassette and number of columns For more information refer to Dispensing parameters on page 38 dispensing 1 Plate type see Step 3 on p 36 If the plate type is correct Dress 96 low DW 22mm If you want to change the 5s plate type press A Select the plate type with 96 DW 44mm 384 low vol 7 5mm 384 low prof 10mm cbc Sasana OK Plate type To accept press Select OK Accept 2 Volume and cassette see Step 4 on p 36 If the volume and the cassette are correct D
3. Installation Installation setups 2 Remove the screws the transport lock piece and the transport lock tag from the screw marked 1 Figure 3 6 Keep the tag for future relocation or transportation of the instrument The transport lock is now released 3 After that fasten the red transport lock piece to the back panel of the dispenser with the same screws for storage Figure 3 7 Keep the transport lock there until needed for future relocation or transportation of the instrument a ee ee a Manufactured in Finland Thermo Fisher Scientific Oy Figure 3 7 Transport lock storage Fastening the 1 Remove the transport lock from the back panel of the instrument transport lock Figure 3 7 2 First fasten the transport lock piece with screw 1 to the pump body Then adjust the pump mechanism height and plate carrier position to fasten screw 2 Figure 3 6 22 Thermo Scientific Multidrop Combi User Manual Thermo Fisher Scientific Installation Operational check Operational Complete the following procedure without the dispensing cassette to check confirm the correct functioning of the dispenser prior to normal use 1 Connect the mains supply cable and switch the instrument on using the mains switch Figure 2 4 e Ifthe instrument starts properly e The display lights up e The plate carrier moves to the home position and the pump lifting mechanism to the up position 2 Install the priming vessel pressin
4. Thermo Scientific Multidrop Combi User Manual Rev 1 5 Cat no NO5616 Copyright 2009 Thermo Fisher Scientific Corporation All rights reserved First edition published in 2005 Printed in Finland Reproduction of the accompanying user documentation in whole or in part is prohibited FILLit is a trademark of Thermo Fisher Scientific Immulon Microfluor Microtiter and Multidrop are registered trademarks of Thermo Fisher Scientific Microlite is a trademark of Thermo Fisher Scientific All other trademarks and registered trademarks are the property of their respective holders Thermo Fisher Scientific reserves the right to change its products and services at any time to incorporate technological developments This manual is subject to change without prior notice as part of a continuous product development Although this manual has been prepared with every precaution to ensure accuracy Thermo Fisher Scientific assumes no liability for any errors or omissions nor for any damages resulting from the application or use of this information This manual supersedes all previous editions Thermo Fisher Scientific shall not be liable for any damages whatsoever arising out of the use or inability to use this product Thermo Scientific microplate instruments are fully guaranteed against defective parts and materials including defects caused by poor workmanship for a period of one year from the date of
5. 36 61 82 85 88 91 103 104 113 needles 28 shaft 27 29 running a protocol 50 S saving a protocol 35 39 44 51 52 53 54 59 73 selecting a protocol 54 selecting columns 13 35 41 82 88 service 63 65 68 103 106 107 109 contracts 65 68 shutdown 62 70 specifications 3 68 69 83 95 96 97 speed 15 40 45 86 95 96 101 startup 35 55 58 101 start up protocol 35 55 58 symbols and markings 4 5 6 Thermo Fisher Scientific T technical specifications 83 95 tension limiting wires 27 28 29 73 74 76 124 tip 26 27 29 30 40 70 71 72 73 91 100 109 123 manifold 72 73 material 26 transport 19 21 22 67 68 95 106 lock 21 22 68 package 19 67 troubleshooting 23 103 107 tubing 25 26 29 30 36 50 62 70 71 72 73 74 75 76 77 79 86 99 109 113 114 115 116 117 118 123 material 26 U unpacking 19 Thermo Fisher Scientific USB 14 56 95 98 110 124 using the keyboard and display 18 32 95 105 V volume 13 15 35 37 39 40 42 43 48 81 82 83 84 85 86 87 88 92 95 96 99 105 113 conversion factor 81 83 range 13 15 40 48 volumetric calibration 91 W warning messages 105 warranty 2 19 106 washing the dispensing cassette 69 WEEE 4 69 97 Thermo Scientific Multidrop Combi User Manual 129 130 Thermo Scientific Multidrop Combi User Manual Thermo Fisher Scientific N
6. 38 Figure 5 38 Fastening the calibration screws 11 Insert the tension limiting wires into their slots into the upper part of the dispensing cassette Figure 5 39 Slots for tension limiting wires Figure 5 39 Calibration screws fastened 12 Replace the upper part cover of the dispensing cassette carefully Figure 5 40 76 Thermo Scientific Multidrop Combi User Manual Thermo Fisher Scientific Thermo Fisher Scientific Maintenance Changing the tubing set Figure 5 40 Replacing the cover of the upper part of the dispensing cassette 13 Ensure that you do not get the tubing trapped or bent between the bottom and upper cover of the upper part of the dispensing cassette Figure 5 41 Figure 5 41 Ensuring the tubing does not get trapped 14 Fasten the three cover retaining screws of the upper part of the dispensing cassette leaving the front screw slightly open Figure 5 42 is ae UES Figure 5 42 Fastening the cover retaining screws 15 Insert the calibration screw cover easier by lifting or bending the upper cover slightly Figure 5 43 Thermo Scientific Multidrop Combi User Manual 77 Maintenance Changing the tubing set Figure 5 43 Inserting the cover on the calibration screws 16 Fasten the front cover retaining screw Figure 5 44 Figure 5 44 Lower and upper part of the dispensing cassette fitted 17 Turn the dispensing cassette so that t
7. S i 1 Switch OFF the instrument Figure 2 4 2 Unplug the instrument immediately from the power supply 3 Carry out appropriate corrective measures However do not disassemble the instrument 4 Ifthe corrective measures taken do not help contact authorized technical service or your local Thermo Fisher Scientific representative Thermo Fisher Scientific Thermo Scientific Multidrop Combi User Manual 63 Routine Operation Emergency situations 64 Thermo Scientific Multidrop Combi User Manual Thermo Fisher Scientific A Instrument maintenance Thermo Fisher Scientific Regular and preventive maintenance gt e Chapter 5 Maintenance This section describes issues related to maintenance of the instrument For reliable daily operation keep the instrument free of dust and liquid spills To prevent unnecessary wear or hazards follow the routine and service procedures described below at the frequency with which they should be applied Clean the outside of the instrument periodically with a cloth dampened with water or a mild detergent or 70 ethanol when necessary Immediately wipe away spilt saline solutions solvents acids or alkaline solutions from outer surfaces to prevent damage Abrasive cleaning agents are not recommended because they are likely to damage the plastic instrument cover Caution The surfaces can be cleaned with most laboratory detergents Dilute the cleaning agent as recommended by the
8. 2 small tube cassettes 10 standard tube cassette to 10000 ul in 1 pl increments For default predispensing values for different plate types refer to Table 4 3 Press OK 48 Thermo Scientific Multidrop Combi User Manual Thermo Fisher Scientific Thermo Fisher Scientific Dispensing direction Routine Operation Protocol settings The row wise dispensing direction is default for each plate type However you may change the dispensing direction if necessary Go to the Settings menu Select the Dispensing direction row settings Row wise direction Column wise direction Select OK Accept Use the Up and Down arrow keys to set the dispensing direction row wise or column wise Note that the whole action is only effective when 384 or 1536 well plate types are selected Press OK With the 96 well plate selection the columns with 8 wells are filled starting from column 1 according to the selected columns after which the plate carrier returns to the home position and the pump lifting mechanism to the up position Row wise and column wise dispensing show no differences in 96 well plate dispensing With the 384 well plate selection the columns with 16 wells are filled in two phases first every other row A C E G O starting from the columns selected and then the pump carrier shifts sideways so that the remaining rows B D F H P are filled in the opposite order If column wise dispen
9. Combi User Manual 67 Maintenance Packing for service Packing for service A Service contracts Disposal of the instrument 68 Thermo Scientific Multidrop Combi User Manual To pack for service follow the guidelines presented below Caution It is important that the instrument is thoroughly decontaminated before it is removed from the laboratory or any servicing is performed on it A When you ship the instrument for service remember to e Inform about the use of hazardous materials e Decontaminate the instrument beforehand Empty the tubings of the dispenser and remove any loose items from the plate carrier for example plates and priming vessels before decontamination e Install the transport lock of the instrument Refer to Fastening the transport lock on page 22 e Pack the instrument according to the enclosed packing instructions e Use the original packaging to ensure that no damage occurs to the instrument during shipping Any damage incurs additional labor charges e Enclose a dated and signed Certificate of Decontamination see Appendix B Certificate of Decontamination both inside and attached to the outside of the package in which you return your instrument or other items e Enclose the return authorization number RGA given by your local Thermo Fisher Scientific representative e Indicate the fault after contacting your local Thermo Fisher Scientific representative or the Thermo Fish
10. and 0 008 standard tube cassette stock solutions absorbance approximately 1 at 540 nm see Table 6 10 Use filtered and preferably fresh reagent Note With small tube cassettes ensure that the dispensed liquid does not contain particles gt 50 um A e A0 02 v v solution of Tween Fluka or equivalent in deionized distilled water Perform the following procedure at two test volumes of 2 pl and 10 ul small tube cassette 20 pl and 100 pl standard tube cassette Table 6 10 Table 6 10 Precision verification procedure data Cassette type Ponceau S Ponceau S stock Tween 0 02 v v stock solution solution Volume dispensed concentration Volume dispensed per well w v per well Small tube cassette 0 4 2 ul 200 ul 0 08 10 ul 190 ul Standard tube cassette 0 04 20 ul 180 ul 0 008 100 ul 100 ul 1 Install the dispensing cassette into the working position so that the lower and upper parts of the dispensing cassette are inserted into their slots 2 Pull the rotor cover over the rotor 3 Ensure that the priming vessel is installed Thermo Scientific Multidrop Combi User Manual 85 Calibration of the Dispensing Cassette Verifying and recalibrating the dispensing cassette 86 Thermo Scientific Multidrop Combi User Manual 10 11 LZ 13 Set the Multidrop Combi to deliver Ponceau S solution by inserting all eight channel tubes into the first Ponceau S stock solution vessel of the two Set the Mu
11. and Low The default speed is High ok Press OK Table 4 5 Dispensing speeds Speed Small tube cassette rpm Standard tube cassette rpm HIGH 900 289 MED 733 259 LOW 567 225 Thermo Scientific Multidrop Combi User Manual 45 Routine Operation Protocol settings Dispensing offset 46 Thermo Scientific Multidrop Combi User Manual The default dispensing offset is 0 0 0 0 mm You can change the x and y axis offset values if the plate used is not the standard one specified in the plate type list see Table 4 3 Go to the Settings menu Select the Dispensing offset row OK Dispensing offset Press OK The track mechanism and pump body move to the setting position Settings 4b XiY offset OK Accept Use the Left and Right arrow keys to set the dispensing offset in the x axis and the Up and Down arrow keys to set it in the y axis direction While you change the offset values the pump body moves accordingly The offset is set in 0 05 mm increments in both the and directions The maximum offset value depends on the selected plate type Press OK If the OK button is not pressed within 60 seconds the adjustments are disregarded and the main view returns to the Settings menu y axis the pump body moves x axis the plate carrier moves Thermo Fisher Scientific Routine Operation Protocol settings Dispensing height The default dispensing height depends on the plate type see Ta
12. any deviations contact your local Thermo Fisher Scientific representative or Thermo Fisher Scientific Oy Visually inspect the transport package the instrument and the accessories for any possible transport damage If the carton has been damaged in transit it is particularly important that you retain it for inspection by the carrier in case there has also been damage to the instrument Neither the manufacturer nor its agents can be held responsible for any damage incurred in transit but the manufacturer will make every effort to help obtain restitution from the carrier Upon receipt of the carrier s inspection report arrangements will be made for repair or replacement If any parts are damaged contact your local Thermo Fisher Scientific representative or Thermo Fisher Scientific Oy Move the packed instrument to its site of operation Unpack the Multidrop Combi instrument and accessories carefully with the arrows on the transport package pointing upwards The following notes and instructions are sent with the instrument and are immediately available when you open the package e Warranty Certificate card e Packing instructions Packing list e Thermo Scientific Multidrop Combi User Manual and Quick Reference Guide e Transportation discrepancy report Retain the original packaging and packing material for future transportation The packaging is designed to assure safe transport and minimize transit damage Use of alternative p
13. bodid erise a a inc teaodieabenrtheeh ae caneneuteds 32 EE E E aera este reat 32 Dip a E E AE T E A 33 Navbatga a A OI 34 Dispens TE onor a ses a E E E a saute 36 Three key selections to start dispensing sessseseesseesesesesseresssesseess 37 Usine Geral so WAE ss cccssstecmctenceemesa sums E A 38 Dispensino parainie ters orari rn anio ann a N 38 PIRE YD ia A TER 38 Thermo Scientific Multidrop Combi User Manual 7 Contents Dispensing cassette and VOlUME eeseeeeeseeeeseeeeeeeeeeeeeesaeeens 40 Gio Chentiecacla old Reem ne reer rere eer eR eer en erect res erry 4 PROOST ES anaa E cnt er Serer tine tree eee 44 IDES Pen Sime Speed erreseina EE E viene 45 PIS PENS CMO TSE Ere tesa cee yeereetees Se een eee 46 Dipen Fassel 6h tod 0 ge nene ret eer eens NT 47 Predispensing VOUI Cisne eases arco ee eae eesti eee 48 Dispens ine GIT SCION aserria hae sii E 49 RATS a pPoO sie cea ne na a ee 50 DAV SAN CCIE ROL CO beran e E E 51 Select PEOLOCO lyiy soia xis nemste nase E E T E a 54 Delete proto colonas renter nn ee eer ee eer torte eee 54 PS CUA O DELO 11S e a E EE E EOE 56 COMPTINE CE a T A E OT 56 REMO MOC enna e E edn tome nee ies eaeaealateeet ae 57 SEALE UP Prot Colere aE A E E E 58 DUZE T ei E EAEE AAEN ETNE 59 EESE AA E E A ATE tans 59 Cassette CalibTANOM cerana a T O 60 Dispensing cassette with SMART option ssssssssseseseressersseresee 61 DCECCM GAVEL E E AE E E A EE E E E 62 LON a r A S 62 Enrere SIC y s tuao on a E A
14. care Always wear hand and eye protections as well as corrosive resistant laboratory coats Warning Observe normal laboratory procedures for handling potentially hazardous samples A Whenever it is likely that the protection against safety hazards has been impaired make the instrument inoperative and secure against any unintended operation The protection is likely to be impaired if for example the instrument e Shows visible damage e Fails to perform the intended functions e Has been subjected to prolonged storage under unfavorable conditions e Has been subjected to severe transport stresses If the Multidrop Combi requires service contact your local Thermo Fisher Scientific representative or the Thermo Fisher Scientific technical service department Do not under any circumstances send the instrument for service without any prior contact It is imperative to indicate the fault and nature of the required service This will ensure a faster return of the instrument to the customer Your local Thermo Fisher Scientific representative or distributor will take care of sending a complaint form that is the Warranty Claim Technical Sheet to the Thermo Fisher Scientific technical service department The Warranty Claim Technical Sheet contains a more detailed description of the fault symptom or condition Give all the necessary information to the distributor who will fill out and forward the Warranty Claim Technical Sheet to the Therm
15. display of the Multidrop Combi 33 Figure 5 23 Pressing liquid through the tlp 71 Figure 5 24 Removing the cover plate of the lower part of the dispensing CAS 5 Glee EEE E EATA E SAPETE A TE LEAT 12 Figure 5 25 Removing the old tip band en 12 Figure 5 26 ip band removed eccccceccesceccscessesseeseestessestnsstestessensenens 72 Figure 5 27 New tip DNC nasisnenrtcntlestateietaatealesttderiatetnalecbocushcantaadesh tate 73 Figure 5 28 New tubing set with the tip band attached cee 13 Figure 5 29 Inserting the tip manifold into the lower part of the dispensing GAS SOI anaE AEA 13 Figure 5 30 Pressing the tube fasteners Into plate 74 Figure 5 31 New tubing inserted into the lower part of the dispensing GSS OU S AE E E E E ieee 14 Figure 5 32 Tension limiting wires attached cccececcccceeeeceteeeeeeees 74 Figure 5 33 Replacing the lower part cover of the dispensing cassette 74 Figure 5 34 Parts belonging to the upper part of the dispensing cassette 75 Figure 5 35 Placing the Sight WINGOW cccccccccceececccteceeseeeesteeseenees 15 Thermo Fisher Scientific Thermo Fisher Scientific Figures Figure 5 36 Pressing the tubing identification plate into place 15 Figure 5 37 Pressing the tube fasteners Into place 00 0 cece 76 Figure 5 38 Fastening the calibration SCrEWS ccceccceecceseeeeeeeeeeeeee 76 Figure 5 39 C
16. for each of the two microplates Absmean Mean absorbance of each well X Abses 96 Record the value of Absmean for each of the two microplates in Table 6 11 Thermo Fisher Scientific Calibration of the Dispensing Cassette Verifying and recalibrating the dispensing cassette 14 Calculate the standard deviation results obtained for each microplate by using the following equation or the STDEVP function in MS Excel or the o function in a scientific calculator and record the results in Table 6 11 Standard Deviation V X Absyeg Absmean 96 15 Calculate the coefficient of variation CV for the Standard Deviation obtained for each microplate and record the results in Table 6 11 Compare the CV values to the criteria in Table 6 10 CV Standard Deviation Abse Absmean x 100 Table 6 11 Photometric test data Microplate A Volume 1 Microplate B Volume 2 Set dispense volume ul ul Mean absorbance well ADSmean Standard Deviation SD of Abstest values CV SD AbStest ADSmean X 100 Calibration If necessary calibration is carried out after performing the accuracy gravimetric verification described above a Note It is recommended to let the dispensing cassette rest for a minimum of 10 hours before recalibration depending on the reagent used A Material and equipment The following materials and equipment are required for calibration requires TOEGAN STANON e Multidrop Combi with the
17. i a Ss sette values OK Accept Press STOP 88 Thermo Scientific Multidrop Combi User Manual Thermo Fisher Scientific 10 12 Calibration of the Dispensing Cassette Verifying and recalibrating the dispensing cassette Open the calibration screw cover Figure 6 49 on the upper part of the cassette Use high purity water Place the tube of channel A Figure 11 54 on page 114 into the reservoir with water at room temperature working temperature Press the PRIME button until the channel is dispensing continuously into the priming vessel Tare an analytical balance to 0 with one empty dry 1 x 12 well microstrip Use disposable gloves when handling and weighing the strips Place the microstrip into the microplate frame on the row corresponding to the channel to be recalibrated from A to H Press the START button to start dispensing Weigh the filled microstrip Figure 6 49 The balance should now read 100 mg 5 mg small tube cassette 1000 mg 20 mg standard tube cassette Figure 6 49 Removing the filled microstrip to be weighed I3 Thermo Fisher Scientific If the balance reads lt 100 mg small tube cassette lt 1000 mg standard tube cassette turn the calibration screw counterclockwise one turn corresponding to approximately 0 1 pl small tube cassette 1 ul standard tube cassette If the balance reads gt 100 mg turn in the opposite direction Figure 6 50 Recheck the de
18. into 384 wells 8 s 5 ul into 384 wells 12 s 10 ul into 384 wells 20 s 20 ul into 384 wells 44 5 150 ul into 384 wells 14 s 1 ul into 1536 wells 26 s 5 ul Into 1536 wells 2 ul 10 typical Thermo Scientific Multidrop Combi User Manual 95 Technical Specifications Safety specifications Safety specifications Live parts A In conformity with the requirements 96 Thermo Scientific Multidrop Combi User Manual Cont General specifications 10 pl 5 typical gt 10 pl 5 typical 0 5 ul CV lt 10 2 ul CV lt 5 10 pl CV lt 3 gt 10 ul CV lt 3 Dispensing precision Standard tube dispensing cassette Dispensing volume 5 2500 ul in 5 ul increments 3 s 10 ul into 96 wells 4 s 20 ul into 96 wells 10 s 100 ul into 96 wells 20 s 300 ul into 96 wells 5 pl 3 typical 20 pl 2 typical 100 pl 1 typical gt 100 pl 1 typical 5 s 5 ul into 384 wells 6 s 10 ul into 384 wells 9 s 20 ul into 384 wells Dispensing speed Dispensing accuracy Dispensing precision 5 pl CV lt 10 20 ul CV lt 1 5 100 ul CV lt 1 gt 100 ul CV lt 1 Dispensing accuracy and precision are valid at 22 C 2 C with deionized distilled water This section describes the safety specifications for the Multidrop Combi instrument The instrument is safe to operate with the covers fitted and these must not be removed during operation The covers protect the use
19. manufacturer Do not expose the surfaces to concentrated acids or concentrated alcohols for prolonged periods of time as damage may occur A It is recommended to service the instrument at least yearly Refer to Service contracts on page 68 If you believe that liquid has entered the Multidrop Combi first switch the instrument off Figure 2 4 and unplug the instrument Carry out corrective measures Refer to Emergency situations on page 63 and Decontamination procedure on page 66 for aid If necessary contact your local Thermo Fisher Scientific representative or the Thermo Fisher Scientific technical service department Refer to Packing for service cc e gt gt on page 68 and Service request protocol on page 106 Warning If any surfaces are contaminated with biohazardous material a mild sterilizing solution should be used A Caution Do not autoclave any part of this instrument except the priming vessel and the dispensing cassettes A Thermo Scientific Multidrop Combi User Manual 65 Maintenance Disposal of materials Disposal of materials Decontamination procedure gt D 66 Thermo Scientific Multidrop Combi User Manual Follow laboratory and country specific procedures for biohazardous or radioactive waste disposal Refer to local regulations for the disposal of infectious material Warning The samples can be potentially infectious Dispose of all used dispensing cassettes plates strip
20. metal tip dispensing cassette Cat no 24073295 Avoid touching the inlet of the tip 3 Fill the 20 ml syringe provided with the cassette with deionized distilled water ethanol or another suitable liquid Thermo Fisher Scientific Autoclaving the dispensing cassette A Changing the Thermo Fisher Scientific tubing set Maintenance Changing the tubing set 4 Attach the syringe with a round filter unit provided and then attach the pink adapter with the short piece of tubing provided onto the filter unit Press liquid through the syringe to wash out any particles from the tubing Attach the short tubing onto the outlet of the tip and press liquid through the syringe Figure 5 23 Figure 5 23 Pressing liquid through the tip To verify the cleaning remove the tubing from the tip outlet and attach it to the inlet Press the liquid through the tip and inspect the jet coming through the tip outlet with the small orifice Insert the tubing back onto the tips and place the tip holder back into the cassette Ensure that the tubing does not twist Avoid touching the tip inlets Close the cover by fastening the four screws Both small tube cassettes can be autoclaved 10 times and the standard tube cassette 50 times depending on the reagent used The autoclaving conditions of the dispensing cassettes are as follows 1 bar pressure at 121 C for 20 minutes Warning After autoclaving the dispensing c
21. reader antenna is harvested by the antenna and used to power up the microchip which then changes the electrical load on the antenna to reflect back its own signals See RFID and RFID tag backflush empty The operation of discharging the contents of the tubing of fluid calibration screw The eight screws under the calibration screw cover that will adjust the length of the tube during calibration of the dispensing cassette Figure 6 50 CE marking Conformit Europ ene European Conformity CE Marking on a product is a manufacturer s declaration that the product complies with the essential requirements of the relevant European health safety and environmental protection legislations the product may be legally placed on the market and thus the CE Marking ensures the free movement of the product within EU decontamination Removal or neutralization of radiologic bacteriological chemical or other contamination dispense To distribute pipette liquid into the wells of the preselected strips or microplate dispensing cassette The dispensing cassette contains eight individual tubes The dispensing cassette is detachable and disposable dispensing height The tip position on top of the microplate The distance between the bottom surface of the plate carrier to the tip of the dispensing tip Table 4 3 EIA Enzyme immunoassay emulation mode Running mode imitating the function of Multidrop 384 or Multidrop M
22. the columns with 32 wells are filled in four phases first every fourth row A E I M AC and then the pump carrier shifts sideways above the rows B F J N AD which are then filled in the opposite order After three shifts the remaining rows D H L P AF are filled Column wise dispensing shifts the pump carrier sideways three times filling 32 wells and then the plate carrier moves the dispensing head over the next column In 384 and 1536 well plate mode row wise and column wise dispensing can be effectively used The Multidrop Combi can be used as a stand alone instrument or controlled with a PC via an RS 232 or an USB serial port as part of a robotic system Thermo Fisher Scientific Introduction to the Multidrop Combi Advantages of using Multidrop Combi Adva ntages of The Multidrop Combi microplate dispenser provides several advantages relating mainly to the principle of operation in that it has using Multidrop nit aia Mae enaga m Combi e Flexible plate height of 5 50 mm Flexible volume range of 0 5 2500 ul e Superior precision covering the whole volume range e Easy to use visual user interface e Dispensing of column specific volumes e Backflush feature e High speed dispensing of microvolumes e Robot compatibility and versatile remote control commands e Autoclavable and detachable dispensing cassette and priming vessel e Cassette lifetime monitoring with Multidrop Combi SMART T
23. worksheet Calibration of the Dispensing Cassette Verifying and recalibrating the dispensing cassette 10 Calculate the net weights of the dispensed strips Net column the dispensed volume per well in mg Dispensed weight per well mg column the dispensed volume per well in pl Measured volume ul column and the inaccuracy percentage Inaccuracy column as in the example table Refer to Table 6 8 If desired you can also calculate the average dispensed volume and inaccuracy percentage for the whole plate 11 Depending on the results obtained perform the calibration described below Precision verification photometric on page 85 Refer to Chapter 7 Technical Specifications Date Temperature Dispensing cassette sn Table 6 7 Volume conversion factor Water volume conversion factor Z with pressure of 1013 mbar Water temperature C Conversion factor 16 0 1 0021 18 0 1 0025 20 0 1 0029 22 0 1 0033 24 0 1 0038 26 0 1 0043 28 0 1 0048 Thermo Scientific Multidrop Combi User Manual 83 Calibration of the Dispensing Cassette Verifying and recalibrating the dispensing cassette Table 6 8 Example of an accuracy verification measured with a small tube cassette at 22 C Channel Strip weight g Dispensed weight Measured volume Inaccuracy Strip of one dispense per pl well mg Tare Strip Net x 1000 Weight per well x Measured 10x5 conversion factor Z volume Set volume x 100 S
24. A 56 Columns and channels for a 12 well plate setup 116 Figure A 57 Columns and channels for a 24 well plate setup 117 Figure A 58 Columns and channels for a 48 well plate setup 118 Thermo Scientific Multidrop Combi User Manual 11 Tables 12 Thermo Scientific Multidrop Combi User Manual Tables Table 4 1 Main types of Thermo Scientific Multidrop Combi dispensing CASS oO ears cc neeriscat teat ttcteich ceased utd eetele aise Patahbact bans dach bahiadtreates 26 Table 4 2 Icons in the MAIN VIEW ccc cceccccccescesseecscescesseneeeesesseeetenenean 35 Table 4 3 Default parameter values for different plate types 00 0 39 Table 4 4 Dispensing volumes of the cassettes 000 cece eee 40 Table 4 5 Dispensing SOCCOS incor cone ceineh aA Mao ER 45 Table 4 6 Total usage of cassettes 00 coe eccceececessetetesestereteeneeeeees 62 Table 6 7 Volume conversion factor eerren 83 Table 6 8 Example of an accuracy verification measured with a small tube Cas GIA ae Ad a ee Pe Pee eT 84 Table 6 9 Accuracy verification results oo cece eee eececesteseeseseeereen 84 Table 6 10 Precision verification procedure data 0 cceeeeeseeeeee 85 Table 6 11 Photometric test data ccc eccccscecesseeescesseseeeeeesterenneen 87 Table 7 12 General specifications oo cece ecceeeceseecesesesesestereteeeeeers 95 Table 9 13 Error Messages reported 103 Tab
25. EU Member State European Country and this product should be disposed of or recycled through them Further information on Thermo Fisher Scientific s compliance with these Directives the recyclers in your country and information on Thermo Scientific products which may assist the detection of substances subject to the RoHS Directive are available at www thermo com WEEERoHS aA Regarding the original packaging and packing materials use the recycling operators known to you For more information contact your local Thermo Fisher Scientific representative This section describes issues related to maintenance of the dispensing cassettes To wash the dispensing cassette follow these steps 1 Wash the tubes by predispensing them with deionized distilled water Make sure all the tubes are washed properly 2 If cleaning with water is insufficient use a mild laboratory detergent and then predispense with large amounts of deionized distilled water 3 Empty the tubings of the dispensing cassette The dispensing cassette can be dried at room temperature 4 After washing store the dispensing cassette in the rest position Figure 4 19 Thermo Scientific Multidrop Combi User Manual 69 Maintenance B Dispensing cassette maintenance A Cleaning the tips DO oe Cleaning the metal tips 70 Thermo Scientific Multidrop Combi User Manual 5 Ifthe instrument is not used for a couple of hours insert the dispensing cas
26. Fisher Scientific Routine Operation Running a protocol The sandglass window will appear while the Save action is in progress F Main 1 Settings Options You can create a maximum of 100 protocols including the factory defined protocols When you save the protocol all the following parameters are saved 55 Se J abcd Thermo Fisher Scientific Thermo Scientific Multidrop Combi User Manual 53 Routine Operation Running a protocol Select protocol To select an existing protocol for a particular plate type first select the plate type see Plate type on page 38 and then select the Save and Edit Protocol row The protocols are listed according to each plate type Full plate Default protocol ZY Select the protocol using the Up and Down arrow keys ok Press OK to accept the selection The protocol is now selected Note In the protocol list only the protocols made for the plate type in question are shown A Delete protocol To delete an existing protocol for a particular plate type first select the plate type see Plate type on page 38 and then select the Save and Edit Protocol row The protocols are listed according to each plate type 54 Thermo Scientific Multidrop Combi User Manual Thermo Fisher Scientific Routine Operation Running a protocol CE Select the protocol using the Up and Down arrow keys oS Press the Right arrow key to enter the edit mod
27. O O after use HGFE NO59 13 01 Figure 11 54 Tube order in the dispensing cassette 114 Thermo Scientific Multidrop Combi User Manual Thermo Fisher Scientific 6 well plate Thermo Fisher Scientific settings 6 48 Well Plate Dispensing Instructions First select a 96 well plate with an appropriate height a A O ay I A a EN sa gros p o Te ee he f Z f o o N Mie t a j j o J a Zz pe a Se J a aa a Figure A 55 Columns and channels for a 6 well plate setup Table A 18 Channel and column settings for a 6 well plate Dispensing cassette Multidrop Combi Channel settings Column settings Channel Tube in tubing weight Column Selection A removed 1 unselected B inplace 2 selected C removed 3 unselected D removed 4 unselected E removed D unselected F in place 6 selected G removed 7 unselected H removed 8 unselected 9 unselected 10 unselected 11 selected 12 unselected Thermo Scientific Multidrop Combi User Manual 115 6 48 Well Plate Dispensing Instructions First select a 96 well plate with an appropriate height f o o a 7 K P P fs at ee OOO ee C G O Figure A 56 Columns and channels for a 12 well plate setup 12 well plate settings Table A 19 Channel and column settings for a 12 well plate Dispensing cassette Channel settings Multidrop Combi Column settings Channel Tube in tub
28. Options serial 835004040 OK Exit 94 Thermo Scientific Multidrop Combi User Manual Thermo Fisher Scientific Chapter 7 Technical Specifications General specifications Thermo Fisher Scientific reserves the right to change any specifications without prior notice as part of our continuous product development program Table 7 12 General specifications General specifications Thermo Fisher Scientific Overall dimensions Weight total Mains power supply Power consumption Heat dissipation Fuses Operating conditions indoor use Transportation conditions Storage conditions Autoclaving conditions of the dispensing cassette and priming vessel Control panel Interface Dispensing volume Dispensing speed Dispensing accuracy Continued 355 mm 14 in W x 330 mm 12 9 in D x 220 mm 8 6 in H Instrument Dispensing cassette 9 1 kg 15 7 Ibs 128 g 0 3 Ib 100 240 Vac 50 60 Hz nominal operating range 90 256 Vac 100 VA max 341 BTU max none 10 C to 40 C 40 C to 70 C packed in transport packaging 25 C to 50 C packed in transport packaging pressure 1 bar temperature 121 C time 20 min autoclavings 10 times small tube cassettes 50 times standard tube cassette Keys arrow keys OK START STOP PRIME EMPTY SHAKE Serial RS 232C USB 1 1 2 0 compatible Small tube dispensing cassette 0 5 50 ul in 0 5 ul increments 5 s 1 ul
29. R 63 Chapter 5 Mamtenaht E sivienn aatapandeaaeaeaenaaeinane enim 65 A Instrument maintenance s sseessesserssersseresersscrsscessresseessesseees 65 Regular and preventive mamitenance sessies 65 DPisp sakor material aenea a 66 Decontamination proced are sorer kso nE 66 Packine for Servie oeiras ee EEE 68 SEVICE CONACS reaa r A NA 68 Disposalot thie Sirane HE eie ea E S 68 B Dispensing cassette maintenanCecicisesinniiisi an 69 Washing the dispensing Cassette esccceesscecesseeeeseeeseseeeeseeeeeaees 69 eat E NS 1 o A A A tone E E E eeeaveeeaeeys 70 Cleaning ne motal 11S cease a eee eee aie 70 Autoclaving the dispensing cassette vacaczsicavaracenvstezacotntncncetudtonvmete 7I Changing the tubing Set cecep nei a peter rte Z1 Changing the tip DaN raa a R T2 Fitting the new tubing set step by step sssesesssssresssessresssrrssrersressss 73 Disposal of the dispensing cassette suet eteeetiie eee 80 Chapter 6 Calibration of the Dispensing Cassette ccssssseeeseeeeeeeees 81 Verifying and recalibrating the dispensing cassette eseeeeseeeeees 81 Accuracy verification gravimetric ceeseeesseesseeeeesceeeeseeeeenneeeees 81 Materials and equipment needed for accuracy verification 81 Accuracy verification PrOCEQULE species co tecset svebantuerestauste ceose teu nteneee 82 Accuracy verification worksheet csisacescvsciinesuccimweeetiosmie 83 Precision verification photometric ccessceceeeseeeee
30. Refer to Table 4 1 Figure 4 10 and Table 10 15 Caution With small tube cassettes ensure that the liquid or reagent does not contain any particles gt 50 pm and that the liquid container is covered Avoid dust or any particles gt 50 um when operating with the dispensing cassette A o Note Do not touch the dispensing cassette tips or the tubing inlet to ensure trouble free dispensing Thermo Fisher Scientific assumes no liability for the use of third party dispensing cassettes A Thermo Fisher Scientific Thermo Scientific Multidrop Combi User Manual 25 Routine Operation Dispensing cassettes Table 4 1 Main types of Thermo Scientific Multidrop Combi dispensing cassettes Dispensing Reagent Tubing Tip Autoclava range pretreatment material material bility 24073290 Small tube plastic tip dispensing preferably filtered lt 50 um and silicone 0 4 mm and inner 10 times cassette blue With SMART option particles 24073292 24073295 Small tube preferably silicone stainless 10 times metal tip filtered 0 4mm steel dispensing lt 50 um ruby cassette particles 0 22 mm gray With SMART Option 24073297 24072670 Standard tube 5 2500 ul silicone 50 times dispensing 1 3mm cassette black With SMART option 24072675 All the cassettes with the SMART option are labeled see Table 10 15 for available dispensing cassettes These dispensing ca
31. Standard tube dispensing cassette 10 pack 24072675 SMART standard tube dispensing cassette with 40 cm tubing set 24072676 SMART Standard tube dispensing cassette 5 pack 24072677 Long standard tube dispensing cassette length of tubing 50 200 cm in 10 cm increments 24072678 SMART long standard tube dispensing cassette 24073293 Long small tube plastic tip dispensing cassette 24073298 Long small tube metal dispensing cassette Thermo Fisher Scientific Thermo Scientific Multidrop Combi User Manual 109 Ordering Information List of accessories List of Table 10 16 Codes for accessories accessories Code N05616 N05640 Item Multidrop Combi User Manual Priming vessel Multidrop Combi N05843 Priming vessel tube assembly and cap 1210550 1210520 2305290 N04001 030003 Cord mains EURO Cord mains UL CSA Serial cable F9 F25 USB A B device cable Tool 3mm DIN911 Allen key handled 95029370 Microtiter 1x12 strip assembled UB flat bottom min order size 95040000 not included 5000 pcs Microtiter 384 plate round well 5 pcs alternative plate option Nunc Cat no 473709 see Table 10 17 List of plates Table 10 17 Codes for plates Code 3395 9502227 1571 9502887 7605 9502867 473 09 Item Quantity 96 well microplates Immulon 1B plate flat bottom 50 pcs box 96 well plate UB flat bottom 50 pcs box Microlite 1 plate flat bottom 50 pcs box White 96 well plate UB flat bottom 50
32. Thermo Scientific Multidrop m z 384 and Thermo Scientific Multidrop Micro command sets when in remote use Thus it enables replacement of an old Multidrop unit with a Multidrop Combi Select the dispenser in remote mode in question Refer to Remote control to Multidrop Combi on page 98 Go to the Options menu Select the Remote mode row settings Options Default protocol 96 sta Instrument settings Calibration OK Remote mode Settings A 5elect OK Accept B G Use the Up and Down arrow keys to select the remote mode in use ok Press OK Thermo Scientific Multidrop Combi User Manual 57 Routine Operation Instrument options Start up protocol You can set which protocol is automatically selected in the Main menu when the Multidrop Combi is powered on Go to the Options menu Select the Start up protocol row Settings Options RS 232 Multidrop Combi Instrument settings Calibration Seimgs Options Default protocol 4 low prof 10mm Default protocol g04 standard ismm l 5elect OK Accept Use the Up and Down arrow keys to select the start up protocol The protocols are listed grouped and indented under each plate type ok Press OK The protocol name is appended with the plate type e S a Options RS 232 Multidrop Combi Instrument settings Calibration OK Start up protocol 58 Thermo Scientific Multidrop Combi User Manual Thermo Fisher Scienti
33. Thermo Scientific Multidrop Combi User Manual Press the OK button every time to select a column The selected column becomes red Use the OK button to toggle between a filled or empty column Use the Down arrow key and select Accept and press OK after selecting all the columns you wish You can press the STOP button to exit the column selection without making any changes The dispensing volume selected in the Volume and cassette window see Dispensing cassette and volume on page 40 is shown in the bottom left box You can adjust the dispensing volume for each individual column Thermo Fisher Scientific Thermo Fisher Scientific Routine Operation Dispensing parameters gD ED ed A ATED ED Ed Accept Volume value box Press the OK button for 0 5 seconds The Volume value box is now activated bright red Adjust the dispensing volume of the column where the cursor is located using the Up and Down arrow keys Et Ea Ea EE ept olume OK Accept STOP Escape Press OK after selecting the desired volume You can press the STOP button to exit the volume selection without making any changes Thermo Scientific Multidrop Combi User Manual 43 Routine Operation Protocol settings Protocol settings 44 Thermo Scientific Multidrop Combi User Manual This section describes the additional parameters that supplement the plate type you have selected These additional
34. ackaging materials may invalidate the warranty Also retain all instrument related documentation provided by the manufacturer for future use Thermo Scientific Multidrop Combi User Manual 19 Installation Requirements Requirements Precautions and limitations 20 Thermo Scientific Multidrop Combi User Manual When you set up your Multidrop Combi avoid sites of operation with excess dust vibrations strong magnetic fields direct sunlight draft excessive moisture or large temperature fluctuations Make sure the working area is flat dry clean and vibration proof and leave additional room for accessories cables reagent bottles and so on Make sure the ambient air is clean and free of corrosive vapors smoke and dust Make sure the ambient temperature range is between 10 C 50 F and 40 C 104 F Make sure relative humidity is between 10 and 80 non condensing Leave sufficient space at least 10 cm 3 9 in on both sides and at the back of the unit to allow adequate air circulation The Multidrop Combi does not produce operating noise at a level that would be harmful No sound level measurements are required after installation Place the instrument on a normal laboratory bench The net weight of the entire equipment is approx 9 1 kg 15 7 lbs and the dispensing cassette 128 g 0 3 Ib The instrument operates at voltages of 100 240 Vac and a frequency range of 50 60 Hz Always ensure that th
35. ain the said document Remote Mode Active Press STOP to exit The instrument has two alternative computer interfaces RS232 and USB Note It is recommended to use USB to improve the reliability of traffic in robot applications A The interface to use is selected from the Multidrop Combi user interface Thermo Fisher Scientific Thermo Fisher Scientific O amp As Chapter 8 Frequently Asked Questions QI Can the dispensing cassettes of the Multidrop Combi be used in the Multidrop 384 and Thermo Scientific Multidrop DW A1 Yes the dispensing cassettes are interchangeable The standard tube cassette Cat no 24072670 can be used in the Multidrop 384 and Multidrop DW The small tube cassettes Cat no 2407390 and 2407395 can be used in the Multidrop Micro Q2 Can the Dispensing cassette machined covers Cat no 24070300 be used in the Multidrop Combi A2 No it cannot be used Q3 How many times can the Multidrop Combi dispensing cassettes be autoclaved A3 Either 10 times small tube cassettes Cat no 2407390 and 2407395 or 50 times standard tube cassette Cat no 24072670 Q4 Can the dispensing cassettes with the SMART option be autoclaved A4 Yes as many times as the cassettes without the RFID tag Q5 What are the autoclaving conditions for the dispensing cassettes A5 The autoclaving conditions are as follows 1 bar pressure at 121 C for 20 minutes Q6 What is the dead volume
36. alibration screws fastened cccceeccceceseesseseseeseteeeeseen 76 Figure 5 40 Replacing the cover of the upper part of the dispensing YS SIS SI E a aie eR AA NA E AAE E A E E L1 Figure 5 41 Ensuring the tubing does not get trapped s 71 Figure 5 42 Fastening the cover retaining SCreWsS eeeen 71 Figure 5 43 Inserting the cover on the calibration SCrews cccceceee 78 Figure 5 44 Lower and upper part of the dispensing cassette fitted 78 Figure 5 45 Dispensing cassette before calibration 0ccceeeeeeeeee 78 Figure 5 46 Precalibrating the dispensing Cassette ccceceeeeeen 79 Figure 5 47 Dispensing cassette precalibrated ccccceeeeeseeseeeeeee 79 Figure 5 48 Inserting the tubes into the PEEK tubing weigMt 719 Figure 6 49 Removing the filled microstrip to be weighed 0 cc 89 Figure 6 50 Screwing a calibration screw according to the calibration FOS WN Gy EE E E E E EIE E E TA 90 Figure 6 51 Placing the tube onto the tip of channel A ssa 91 Figure 6 52 Adjusting the liquid level first to the lower mark and then to WEUPE ILA a cet hori cine near eatn 92 Figure 6 53 Screwing the calibration screws according to the calibration FOS NES es sasccasts cc A AAAA 93 Figure 11 54 Tube order in the dispensing Cassette 0 0cceeeeeeeee 114 Figure A 55 Columns and channels for a 6 well plate setup 000 0 115 Figure
37. ard 10 5 mm Small 13 11 5 You can dispense without warning to the maximum dispensing volume but once the maximum is exceeded a warning appears and you have to press OK to continue dispensing The default dispensing height is 1 mm above the selected plate Note If the default plate values do not agree with yours change the dispensing offset and height values under the Settings menu tab You can save the plate values for future use A Note You can also get new plates for use by transferring them from FILLit Software A Thermo Scientific Multidrop Combi User Manual 39 Routine Operation Dispensing parameters Dispensing cassette There are two types of dispensing cassettes available Small tube cassette and volume either plastic tip or metal tip and Standard tube cassette Refer to Dispensing cassettes on page 25 and Table 10 15 Each dispensing cassette type covers a specific volume area Go to the Main menu Select the Volume and cassette row O 384 standard 15mm OK Volume and cassette Press OK The default cassette type and dispensing volume for the selected plate type are shown Max volume Small Standard tube a tube cassette 3 cassette amp i I Min volume Volume lt 4 gt Cassette OK Accept First select the cassette type using the Left and Right arrow keys Then select the dispensing volume using the Up and Down arrow keys You can speed up the selection by holding down the arr
38. assette must cool down at least 2 hours to room temperature before use A With the Standard tube dispensing cassette the tubing set can be changed according to the instructions below First the tip band is changed and then the new tubing set is fitted Thermo Scientific Multidrop Combi User Manual 71 Maintenance Changing the tubing set Changing the tip band 1 Place the dispensing cassette onto the laboratory bench with the dispensing tips pointing down Lyp 2 Unfasten the four cover retaining screws of the lower part of the 72 Thermo Scientific Multidrop Combi User Manual dispensing cassette Figure 5 24 M Figure 5 24 Removing the cover plate of the lower part of the dispensing cassette 3 Remove the tip manifold the tube fasteners and the rest of the tubing from the lower part of the dispensing cassette Then detach the tip band from the tubing by pulling the tubes away from the tip band Figure 5 25 Figure 5 25 Removing the old tip band 4 Replace the tip band Figure 5 26 The tips of the tip band must be clean to function correctly Figure 5 27 Figure 5 26 ip band removed Thermo Fisher Scientific Maintenance Changing the tubing set Insert tube here Tip Figure 5 27 New tip band Fitting the new 1 First place the new tubing set onto the laboratory bench with the tubing set step by tip band attached Figure 5 28 Ensure that you save the eight s
39. ates and strips in 96 384 or 1536 well plate format Use for self testing is excluded The Multidrop Combi Figure 1 1 is an automatic programmable eight channel microplate bulk reagent dispenser for microvolume dispensing It has a peristaltic pump for rapid and continuous dispensing of liquids into various microplates It can dispense 1 to 8 different reagents from external liquid reservoirs into different rows and can be used in several applications for example in drug discovery high throughput screening genomic proteomic and cell based assays and ELISA Multidrop Combi with SMART option is equipped with an inbuilt tracking system that counts the exact lifetime of the dispensing cassette With a volume range of 0 5 to 2500 ul for 96 384 and 1536 well plates with plate heights from 5 to 50 mm the Multidrop Combi offers outstanding flexibility for a wide range of applications Also 6 48 well plates as well as tubes in a 96 channel rack can be used The Multidrop Combi is lightweight transportable and compact on a laboratory bench It can dispense 1 pl into the entire 384 well microplate in 5 seconds or 1 pl into the entire 1536 well microplate in 14 seconds An additional feature is that each column can be programmed to have individual volumes Note that the whole microplate does not have to be filled Refer to Column selection on page 41 Thermo Scientific Multidrop Combi User Manual 13 Introduction to the Multidr
40. be further used but in this case the user has to monitor the cassette tube wearing with care The serial number of the cassette is shown on the info text bar Options Last calibration date 18 Oct 2005 Cassette type Small plastic tip Since last calibration 1 Total usage Thermo Scientific Multidrop Combi User Manual 61 Routine Operation screen saver Screen saver Shutdown o 62 Thermo Scientific Multidrop Combi User Manual Table 4 6 otal usage of cassettes Small tube cassette Standard tube cassette 100 usage 100 usage 1000 pcs of 384 well plates 5 ul 3000 pcs of 96 well plates 100 ul 50 usage 50 usage 500 pcs of 384 well plates 5 ul 1500 pcs of 96 well plates 100 ul 25 usage 25 usage 250 pcs of 384 well plates 5 ul 750 pcs of 96 well plates 100 ul If you dispense 1 ul into 1536 well plates with the small tube cassette then the 100 usage equals approximately 1200 plates The Multidrop Combi screen saver shown below appears when the instrument has not been used for 20 minutes You can deactivate the screen saver and return to the previous display by pressing any key SCIENTIFIC To shut down the Multidrop Combi follow these steps Warning Remove any microplates still on the instrument Dispose of all microplates and strips as biohazardous waste A 1 After all the plates have been dispensed press the EMPTY button to return the reagent or liquid from the tubes t
41. ble 4 3 You can change the dispensing height values if the UT plate used is not the standard one specified in the plate type list Go to the Settings menu Select the Dispensing height row f E Settings WOptions OK Dispensing height The track mechanism and pump body move to the setting position Move OK Accept D E gt Use the Up and Down arrow keys to set the dispensing height While you change the height the pump body moves accordingly The height is set in 0 05 mm increments in both the up and down directions The minimum height value depends on the selected plate type see Table 4 3 The default dispensing height is 1 mm above the selected plate Press OK If the OK button is not pressed within 60 seconds the adjustments are disregarded and the main view returns to the Settings menu Thermo Fisher Scientific Thermo Scientific Multidrop Combi User Manual 47 Routine Operation Protocol settings Predispensing You can change the predispensing volume the volume that is volume automatically dispensed before the start of plate filling if necessary Some more viscose solutions used may sometimes require extra predispensing Go to the Settings menu Select the Predispense Prime row Main Settings WOptions ar C Speed HIGH Disp offset 0 0 0 0mm Predispense volume OK Accept eG eS Use the Up and Down arrow keys to set the predispensing volume The volume ranges from
42. delivery Thermo Fisher Scientific will repair or replace defective parts or materials during the term of warranty at no extra charge for materials and labor provided that the products were used and maintained in accordance with Thermo Fisher Scientific s instructions The warranty is invalid if products have been misused or abused For the warranty to be effective the product must have been purchased either directly from Thermo Fisher Scientific or from an authorized Thermo Fisher Scientific distributor The guarantee is not transferable to a third party without prior written approval from Thermo Fisher Scientific This guarantee is subject to the following exclusions e Any defects caused by normal wear and tear e Defects caused by fire lightning flood earthquake explosion sabotage war riot or any other occurrence of the type listed above e Refurbished products that are subject to different warranty conditions THIS WARRANTY IS IN LIEU OF ALL OTHER EXPRESSED OR IMPLIED WARRANTIES INCLUDING BUT NOT LIMITED TO ANY IMPLIED WARRANTIES OF MERCHANTABILITY OR FITNESS FOR A PARTICULAR PURPOSE The seller is not liable for any loss or damage arising out of or in connection with the use of the product or other indirect damages These warranty terms and conditions can be obtained from your local Thermo Fisher Scientific dealer Consumables are not included in the warranty About This User Manual Intended users This user manual is written f
43. dispensing cassette to be verified inserted e Analytical balance resolution at least 0 1 mg preferably 0 01 mg e Calibrated thermometer readable to 0 5 C e 96 well plate with 8 x 12 well microstrips for example Microtiter 1 x 12 Strip Assembled Universal Binding Flat Cat no 95029370 or another similar 8 x 12 strip plate e Disposable gloves e High purity water deionized distilled water Calibration procedure 1 Install the dispensing cassette into the working position so that the lower and upper parts of the dispensing cassette are inserted into their slots Figure 4 14 Thermo Fisher Scientific Thermo Scientific Multidrop Combi User Manual 87 Calibration of the Dispensing Cassette Verifying and recalibrating the dispensing cassette 2 Pull the rotor cover over the rotor If the instrument is equipped with the SMART option and a SMART cassette is being used the calibration data window appears and the calibration data can be viewed ok Press the OK or STOP button to deactivate the calibration data window 3 Ensure that the priming vessel is installed 4 Set the Multidrop Combi parameters as follows see Dispensing parameters on page 38 Plate type 96 standard 15mm Dispensing volume and cassette 10 pl small tube cassette 100 pl standard tube cassette Selected columns 1 10 5 Select Options gt Calibration gt Gravimetric Main Settings Options Std tube volumetric pu J y ee
44. e Delete button a gt oS To delete the protocol use the Delete button Use the Down arrow key and if needed the Left and Right arrow keys to select the Delete Delete ok button and then the OK button to delete the protocol You cannot delete default protocols the Delete button is not activated Also if you have selected a protocol to be a start up protocol it cannot be deleted The protocol is now deleted Thermo Fisher Scientific Thermo Scientific Multidrop Combi User Manual 55 Routine Operation Instrument options Instrument This section describes the instrument parameters All these parameters ti are set in the Options menu The values shown in the Options menu op IONS remain in the instrument memory and are instrument specific not protocol specific routine use A Note You do not normally have to change the instrument options in Computer interface There are two types of computer interfaces available RS 232 and USB Go to the Options menu Select the Computer interface row E a a Options Multidrop Combi Default protocol 96 sta Instrument settings Calibration T oo L 5elect OK Accept ZY gt Use the Up and Down arrow keys to select the computer interface in use ok Press OK 56 Thermo Scientific Multidrop Combi User Manual Thermo Fisher Scientific Thermo Fisher Scientific Routine Operation Instrument options Remote mode The Multidrop Combi can emulate the
45. e local supply voltage in the laboratory conforms to that specified on the rating label on the back of the instrument Figure 2 4 Do not smoke eat or drink while using the Multidrop Combi Wash your hands thoroughly after handling test fluids Observe normal laboratory procedures for handling potentially dangerous samples Wear proper protection clothing such as disposable gloves laboratory coats and so on according to good laboratory practice Ensure that the working area is well ventilated Never spill fluids in or on the equipment Thermo Fisher Scientific Installation Installation setups Installation This section describes the installation setups that you must carry out before operating or relocating the instrument setups i a Warning Ensure that the mains switch Figure 2 4 at the left of the back panel is in the OFF position Also ensure that the mains supply cable is disconnected A Releasing the The instrument has a transport lock Figure 3 5 transport lock Make sure the red transport lock is released before operating the instrument Figure 3 5 Transport lock and transport lock tag present 1 Unscrew the two screws marked 1 and 2 of the transport lock with the hexagonal screwdriver supplied Figure 3 6 so that the track mechanism is loosened o B Screw 1 Screw 2 Figure 3 6 Transport lock fastened Thermo Fisher Scientific Thermo Scientific Multidrop Combi User Manual 21
46. e screen during the startup animation Q19 Can the SMART cassettes with RFID tags be used with the standard Multidrop Combi without SMART option or vice versa A19 Yes but then the calibration data cannot be viewed and the information about the cassette is not updated Also standard cassettes can be used with the Multidrop Combi with SMART option Thermo Scientific Multidrop Combi User Manual 101 Frequently Asked Questions Q amp As 102 Thermo Scientific Multidrop Combi User Manual Thermo Fisher Scientific Chapter 9 Troubleshooting Guide o Note Do not use the instrument if it appears that it does not function 1 properly A Error and When an error is detected the current operation is terminated After an error it is best to abort the current run and restart from the beginning warning codes after the problem is fixed The Multidrop Combi internal software has the following error messages Table 9 13 Table 9 13 Error messages reported Code Error Cause Action 4 Pump position error Peristaltic pump cannot rotate Power off on or contact service D Plate X position error Plate carrier cannot move Power off on or contact service 6 Plate Y position error Plate carrier cannot move Power off on or contact service 7 Z position error Pump lifting mechanism cannot move Power off on or contact service 11 No more memory for Too many protocols saved Delete protocols that are not used storing user data 14 Cannot dispen
47. ececesescesestereseeeereseen 18 Figure 3 5 Transport lock and transport lock tag present 0c cee 21 Figure 3 6 Transport lock fastened 000 cceeccececcsceseesescesestesetesseeeseenens 21 Figure 3 7 Transport lock storage oo cee cecececcccesceseeseeceeeeseessneetestestensenens 22 Figure 3 8 Inserting the priming vessel eeen 23 Figure 4 9 Priming vessel Inserted into place oo ee cecceeeeeeeeeseereeeeeeees 25 Figure 4 10 Parts of a dispensing cassette ooo ce ceecceceesceeeseseeeseerees 26 Figure 4 11 Inserting the dispensing cassette 0 0 0 cee cceeeeeseerseeeees 2 Figure 4 12 Inserting the lower part of the dispensing cassette into Its 27 Figure 4 13 Inserting the upper part of the dispensing cassette 20 Figure 4 14 Lower and upper parts of the dispensing cassette inserted SVENIY MTO UNGIE SOUS sca Battactscenaa ce a hace shat 28 Figure 4 15 Correct placement of all the dispensing cassette tubes 29 Figure 4 16 Pulling the tip protection off from the dispensing cassette 29 Figure 4 17 Removing the tubing weight protection and rubber holder 29 Figure 4 18 Cassette with the tip and tubing weight protections removed30 Figure 4 19 Rest position of the dispensing cassette 0c cece 30 Figure 4 20 Pulling the rotor cover over the rotor oo cceeeeeesceceseeeeeees 31 Figure 4 21 Keyboard of the Multidrop Combi s e 32 Figure 4 22 Main view on the
48. eneeeeeeeeeeeees 85 8 Thermo Scientific Multidrop Combi User Manual Thermo Fisher Scientific Contents Materials and equipment required for precision verification 85 Precision verification Proced Ute otnnspeet ueceteuindeas ie 85 Caubianon Cra VIG ITIC eona R 87 Material and equipment required for calibration eeee 87 Ca DEAL IOn PLO CEC UTS panaia E mse 87 Calibration standard tube volumetric ccceececssececesecceeeeeeees 91 Chapter 7 Technical Specifications cccsssssssesessssseeesessssseseessesesseeeanaess 95 Generals DC CIITCA CIOS ersin renee se ati E retueaantas 95 Sale SPENCE eeiaaatoes 96 TVS SAS E E A E E A EE AE a EAA uasiees 96 In conformity with the Tequirements vesesesicensascev oracasverneciabenraneeyssnes 96 Remote control to Multidrop Combi sssssssesssssesssseresseresssrssseresses 98 Chapter 8 Frequently Asked Questions cccscssssssesssesesseseeseesseesesseeseeeeanaes 99 CSCS E A A EET E A E EA E A N EET 99 Chapter 9 Troubleshooting Guide sccsssssssseeessssssesssssseessseesesssesaeseesnserseeas 103 Errorand Warne cOd eSa a a 103 H a T ee ENT ne eee ee 105 S rele go EE AE re rent RONNIE SENET ENO SET re ern TONE Ie ROTO 105 IVES C hala ja5 ciicsSodvsusaeadesesoulsecd statenadseadeseereatenesouteedseataeeiecteeets 105 Eovronmenta lissen T se aauseseteaeen wes 106 Detectsand abnormal StleSSCS5 0 26 ohceducasterahenadonesccieusarsBetene
49. ension limiting wires attached 5 Fasten the four cover retaining screws of the lower part of the dispensing cassette Figure 5 33 AS m hY b Figure 5 33 Replacing the lower part cover of the dispensing cassette 6 Once the lower part of the dispensing cassette has been fitted place the remaining parts belonging to the upper part of the dispensing cassette onto the laboratory table Figure 5 34 Thermo Fisher Scientific Thermo Fisher Scientific Maintenance Changing the tubing set Figure 5 34 Parts belonging to the upper part of the dispensing cassette 7 Place the plastic sight window with the grooves pointing upwards onto the bottom cover of the upper part of the dispensing cassette if it is not already there Figure 5 35 Figure 5 35 Placing the sight window 8 Press the white tubing identification plate into place on the right hand side of the bottom cover of the upper part of the dispensing cassette Figure 5 36 Figure 5 36 Pressing the tubing Identification plate into place 9 Press each tube fastener into place in its groove on the plastic sight window Figure 5 37 Thermo Scientific Multidrop Combi User Manual 75 Maintenance Changing the tubing set a SA By S SN a git Sg oy BY A Ay ay Figure 5 37 Pressing the tube fasteners into place 10 Fasten the calibration screws from the previous tubing into their places using a hexagonal screwdriver Figure 5
50. er Scientific technical service department Refer to General specifications on page 95 for details on storage and transportation temperatures It is recommended to maintain and service the instrument regularly every 12 months on a contract basis by the manufacturer s trained service engineers This ensures that the product is properly maintained and gives trouble free service Contact the Thermo Fisher Scientific technical service department for more details If the Multidrop Combi has to be disposed of follow the guidelines below Thermo Fisher Scientific B Dispensing cassette maintenance Washing the dispensing cassette Thermo Fisher Scientific Maintenance B Dispensing cassette maintenance Warning Decontaminate the instrument before disposal Refer to Decontamination procedure on page 66 and Certificate of Decontamination on page 107 about decontamination A pag Follow laboratory and country specific procedures for biohazardous or radioactive waste disposal Dispose of the instrument according to the legislation stipulated by the local authorities concerning take back of electronic equipment and waste The proposals for the procedures vary by country Pollution degree 2 see Safety specifications on page 96 Method of disposal Electronic waste Contaminated waste Infectious waste WEEE symbol Thermo Fisher Scientific has contracted with one or more recycling disposal companies in each
51. et volume a 38707 4 3876 05169 10 3383 10 37 B 3869 4 3832 0 5133 10 2667 10 30 o fow es osm os fom f e fom eam osie fromm oe e e fome eam Jose oa wz ifaw Table 6 9 Accuracy verification results Channel Strip weight g Dispensed weight Measured volume Inaccuracy Strip of one dispense per pl well mg Strip Net x 1000 Weight per well x Measured 10x 5 small conversion factor Z volume Set cassette 10 x 1 volume x 100 standard cassette Set volume Mean whole plate Average of channels A H 84 Thermo Scientific Multidrop Combi User Manual Thermo Fisher Scientific Materials and equipment Precision verification photometric required for precision verification Precision verification Thermo Fisher Scientific procedure Calibration of the Dispensing Cassette Verifying and recalibrating the dispensing cassette This section describes the materials and steps needed for precision verification The following materials and equipment are required for precision verification e Microplate photometer set to measure absorbance at 540 nm measurement range 0 2 Abs resolution 0 001 Abs e Two 96 well plates for example Thermo Scientific Microtiter Assembly Strip 1 x 8 Cat no 95029350 or Thermo Scientific Microtiter Microplate Cat no 9502227 e Ponceau S reagent BDH product 341362T or equivalent you will need 0 4 and 0 08 small tube cassette 0 004
52. face 35 56 98 consumables 2 contrast and buzzer 35 59 control panel 32 95 105 Thermo Fisher Scientific D dead volume 99 decontamination 63 65 66 67 68 69 107 119 123 deep well plates 110 default 39 40 41 44 45 46 47 48 49 55 protocols 55 values 39 deleting a protocol 54 103 dispensing 36 37 38 39 40 41 42 113 114 115 116 TI 1138 123 accuracy 95 96 direction 35 49 height 35 39 47 123 offset 35 39 46 parameters 37 38 82 88 precision 96 range 26 speed 15 35 40 45 86 95 96 101 volume 36 37 39 40 41 42 43 82 87 88 95 96 105 113 disposal of 66 68 80 dispensing cassette 80 instrument 68 materials 66 disposal of dispensing cassette 80 disposal of instrument 68 disposal of materials 66 E editing a protocol 35 38 44 51 52 53 54 55 EIA 66 123 empty 32 36 42 62 63 67 68 69 70 81 86 89 93 95 113 114 123 emulation mode 123 EN 97 98 123 environmental requirements 20 error messages 103 EU 69 123 Thermo Scientific Multidrop Combi User Manual 127 F feedback form 121 G glossary 61 123 guidelines for use 6 H hazards 4 65 105 106 119 home position 14 23 32 49 icons 34 35 IEC 123 info text 33 34 61 installation 3 19 20 21 23 27 68 73 82 85 87 91 121 intended use 3 13 internal software 3 32 38 101 103 105 K keyboa
53. fic Routine Operation Instrument options When the instrument is started up the next time the plate type and the protocol are those just selected Buzzer You can choose whether or not the instrument produces a tone for different functions Note that all functions make a certain sound Go to oil the Options menu Select the Buzzer Instrument settings row NSS Options RS 232 Multidrop Combi Default protocol 384 s Calibration OK Buzzer Settings Options d Select OK Accept Use the Left and Right arrow keys to select whether the buzzer audible tone is On or Off Press OK Accept Save symbol The sandglass window will appear while the Save action is in progress Thermo Fisher Scientific Thermo Scientific Multidrop Combi User Manual 59 Routine Operation Instrument options Main Selfings Options v Select OK Accept Cassette calibration It is possible to calibrate the cassettes for different fluids There are two different calibration modes available Gravimetric and Standard tube fo volumetric see Verifying and recalibrating the dispensing cassette on page 81 Go to the Options menu Select the Calibration row T Options Multidrop Combi Default protocol 384 s Instrument settings Main Settings Options 5elect OK Accept CZ Use the Up and Down arrow keys to select the calibration mode If a SMART cassette is used the calibration data can be vie
54. g it over a snap lock Figure 3 8 Figure 3 8 Inserting the priming vessel 3 Pull the rotor cover over the rotor Figure 4 20 4 Press PRIME e The lifting mechanism is lowered and the pump rotates as long as you press PRIME 5 Press START r The dispensing protocol selected with the user interface is started If the protocol or predispense is not started follow the instructions on the display or check Chapter 9 Troubleshooting Guide Thermo Fisher Scientific Thermo Scientific Multidrop Combi User Manual 23 Installation Operational check 24 Thermo Scientific Multidrop Combi User Manual Thermo Fisher Scientific Chapter 4 Routine Operation Priming vessel Ensure that the priming vessel is inserted correctly into its slot on the left of the plate carrier You have fastened it correctly when you slide it over a snap lock Make sure you have a tube assembly or a cap inserted into the drain of the priming vessel Figure 4 9 or a vessel underneath the drain Safety grooves designed to slope and prevent fluid from entering the instrument Priming vessel with a crest in the middle where the priming liquid is aimed at to avoid splashing and a drain Figure 4 9 Priming vessel inserted into place D ispensing The Multidrop Combi can be used with dispensing cassettes with cassettes different tubing sizes The different dispensing cassettes of the Multidrop Combi are presented below
55. he eight sight strips are visible Figure 5 45 Look at the eight sight strips in the upper part of the dispensing cassette You will notice that the tubes are not precalibrated First open the calibration screw cover For precalibration of the dispensing cassette adjust the tube fasteners in the sight strips roughly to the middle using a hexagonal screwdriver Figure 5 46 Sight strips Figure 5 45 Dispensing cassette before calibration 78 Thermo Scientific Multidrop Combi User Manual Thermo Fisher Scientific Thermo Fisher Scientific Maintenance Changing the tubing set Figure 5 46 Precalibrating the dispensing cassette 18 When the tube fasteners have all been adjusted roughly to the middle of the sight strip close the calibration screw cover Figure 5 47 Figure 5 47 Dispensing cassette precalibrated 19 Insert the eight tubes into the PEEK tubing weight either at this stage or earlier Figure 5 48 The new tubing set has now been successfully fitted Figure 5 48 Inserting the tubes into the PEEK tubing weight 20 Calibrate the dispensing cassette according to the instructions in Chapter 6 Calibration of the Dispensing Cassette Thermo Scientific Multidrop Combi User Manual 79 Maintenance Disposal of the dispensing cassette Disposal of the Dispose of the dispensing cassette according to the legislation stipulated di spen sin g cass ette by the local authorities concerni
56. he menus and use the Left and Right arrow keys The main views of each menu tab are shown below RSET Options USB Settings O Speed HIGH Disp offset 0 0 0 0mm CET Disp height 45 00mm Predispense 20pl si Direction OK Pump speed Multidrop Combi Default protocol 96 sta Instrument settings Calibration OK Computer interface Thermo Fisher Scientific Routine Operation Control panel Table 4 2 Icons in the main view Menu tab Icon Function Plate type on page 38 i Main Dispensing cassette and volume on page 40 iy uU Column selection on page 41 Save and edit protocol on page 51 Dispensing speed on page 46 Dispensing offset on page 46 Dispensing height on page 47 Predispensing volume on page 48 Dispensing direction on page 49 Computer interface on page 56 Options Remote mode on page 57 Start up protocol on page 58 Buzzer on page 59 Cassette calibration on page 60 Thermo Fisher Scientific Thermo Scientific Multidrop Combi User Manual 35 Routine Operation Dispensing Dispensi Ng You can immediately start dispensing by following the quick start guidelines below Routine dispensing only requires to key in a few buttons l Power ON the instrument 2 Insert the correct dispensing cassette and the priming vessel Close the P ON rotor cover er
57. he protocol 50 Thermo Scientific Multidrop Combi User Manual Thermo Fisher Scientific Routine Operation Running a protocol Save and edit Go to the Main menu Select the Save and Edit Protocol row protocol oo te TSD LL r J my Ee aoa E x Per eS ee ee ee i nF 5 lt 4h Move OK Add STOP Escape Enter the name of your protocol CS ED a gt oS Use the arrow keys to choose the character you want ok Press OK to enter the said character Thermo Fisher Scientific Thermo Scientific Multidrop Combi User Manual 51 Routine Operation Running a protocol A Aer a D dus 5 3 3 Backspace button 52 Thermo Scientific Multidrop Combi User Manual The available characters are a to z 0 to 9 hyphen underline _ and space Note Use max 20 characters including the space character Some of the characters are broader than the others whereby some of the names you edit will display less than 20 characters in the protocol list shown as three dots at the displayed name The protocol name is automatically capitalized A 4H Move OK Add STOP Escape To remove characters use the Backspace button Use the Down arrow key and if needed the Left and Right arrow keys to select the Backspace button and then the OK button to remove the character s 4b Move OK Save STOP Escape Use the Down arrow key to select the Save button and then press the OK button to save the edited protocol name Thermo
58. hermo Fisher Scientific Thermo Scientific Multidrop Combi User Manual 15 Introduction to the Multidrop Combi Advantages of using Multidrop Combi 16 Thermo Scientific Multidrop Combi User Manual Thermo Fisher Scientific Chapter 2 Functional Description Instrument This section shows the front and back views of the Multidrop Combi ay 0 ut instrument Front view The front view of the Multidrop Combi instrument is shown in Figure 2 2 Display Rotor cover Pump body l _ Priming vessel Plate carrier Figure 2 2 Multidrop Combi front view Thermo Fisher Scientific Thermo Scientific Multidrop Combi User Manual 17 Functional Description Instrument layout The close up of the Multidrop Combi keyboard and display is shown in Figure 2 3 q SCIENTIFIC Display Keyboard ion ert te a R T Figure 2 3 Close up of the Multidrop Combi keyboard and display Back view The back view of the Multidrop Combi instrument is shown in Figure 2 4 WEEE symbol Mains connector Mains switch Instrument label Serial connector USB Figure 2 4 Multidrop Combi back view 18 Thermo Scientific Multidrop Combi User Manual Thermo Fisher Scientific Delivery check Checking delivery Thermo Fisher Scientific Unpacking Chapter 3 Installation This section covers the relevant procedures to be carried out on receipt of the instrument Check the enclosed packing list against order In case of
59. hyde solution or another agent recommended by your safety officer Thermo Fisher Scientific Maintenance Decontamination procedure 2 Empty the reagent container Ensure that you are wearing disposable gloves 3 Switch OFF the power Figure 2 4 and disconnect the mains supply cable 4 Disinfect the outside of the instrument using a cloth dampened with 70 ethanol 5 Place the dispensing cassette for example in 1 3 Virkon solution or 4 glutaraldehyde solution for 10 minutes and rinse it with deionized distilled water The dispensing cassette can alternatively be autoclaved 6 Place the instrument in a large plastic bag 7 Place a cloth soaked in the prepared solution into the bag Ensure that the cloth does not come into contact with the instrument 8 Close the bag firmly and leave the instrument in the bag for at least 24 hours 9 Remove the instrument from the bag 10 Clean the instrument using a mild detergent 11 Remove any stains using 70 ethanol 12 Flush the fluid path of the instrument with deionized distilled water using the predispensing procedure 13 Wash and disinfect the reagent containers 14 After performing this decontamination procedure enclose a signed and dated Certificate of Decontamination both inside the transport package and attached to the outside of the package see Appendix B Certificate of Decontamination Thermo Fisher Scientific Thermo Scientific Multidrop
60. icro EN European Norm Thermo Fisher Scientific EU European Union home position The plate carrier is positioned to the furthest right of the transfer rails IEC International Electrotechnical Commission PEEK Abbreviation for polyetheretherketone It is a plastic material predispense The operation of filling a pump intake with fluid to expel the air predispensing priming Completely filling the dispenser tubing with bubble free fluid to allow sustained reproducible dispensing action The air in an unpredispensed line acts as a spring adversely affecting accuracy and precision remote control Running mode allowing a remote computer to operate the dispenser RFID Radio frequency identification or RFID is an automatic identification method relying on storing and remotely retrieving data using devices called RFID tags or transponders An RFID tag is a small object that can be attached to or incorporated into a product RFID tags contain silicon chips and antennas to enable them to receive and respond to radiofrequency queries from an RFID transceiver See antenna and RFID tag RFID tag A microchip attached to an antenna that is packaged in a way that it can be applied to an object The tag picks up and sends signals to a reader The tag contains information such as a serial number and parameters related to the product where the tag is attached Tags come in many forms such smart labels that can have a barc
61. ing weight Column Selection A removed 1 unselected B inplace 2 selected C removed 3 unselected D inplace 4 unselected E removed 5 selected F removed 6 unselected G inplace 7 unselected H removed 8 selected 9 unselected 10 unselected 11 selected 12 unselected 116 Thermo Scientific Multidrop Combi User Manual Thermo Fisher Scientific 24 well plate Thermo Fisher Scientific settings 6 48 Well Plate Dispensing Instructions First select a 96 well plate with an appropriate height ie AN A SIGs ieee SOOCOG OOOOOO Figure A 57 Columns and channels for a 24 well plate setup Table A 20 Channel and column settings for a 24 well plate Dispensing cassette Channel settings Channel Tube in tubing weight Column in place removed in place removed removed in place removed Q ontiml uololw amp in place A N Multidrop Combi Column settings Selection selected unselected selected unselected selected unselected unselected selected unselected O I o co ym s my ory amp W RO selected unselected selected Thermo Scientific Multidrop Combi User Manual 117 6 48 Well Plate Dispensing Instructions 48 well plate settings 118 Thermo Scientific Multidrop Combi User Manual First select a 96 well plate with an appropriate height E i OOOOOOOO 8880888 OQOQQOOOO OOOOOOOO QQQQQQ28 OODCOOOO
62. ispensing parameters required to paramete rs create and edit a protocol All these parameters are set in the Main menu parameters are dependent on the plate type A Note Make the plate type selection first because all the other Caution Make sure the plate lid if used is removed before dispensing A Plate type Go to the Main menu 38 Thermo Scientific Multidrop Combi User Manual Thermo Fisher Scientific Thermo Fisher Scientific Routine Operation Dispensing parameters 96 low DW 22mm 96 DW 44mm 364 low vol 7 5mm 364 low prof 10mm obci aaa SHAT Select OK Accept CZ E gt Select the plate type using the Up and Down arrow keys 3 E ok Press OK to accept the selection The available plate types and the default parameter values are given in Table 4 3 Each plate type has a selected default dispensing cassette type which is automatically set by the instrument Table 4 3 Default parameter values for different plate types Plate type Cassette Max Dispensing type dispensing height mm volume pl 96 well standard 15 mm Standard 500 16 96 well low DW deep well 22 mm Standard 600 23 96 well DW 44 mm Standard 2500 45 384 well low volume 7 5 mm Small vas 8 5 384 well low profile 10 mm Small 70 11 384 well standard 15 mm Small 130 16 384 well DW 22 mm Standard 200 23 384 well DW 44 mm Standard 300 45 1536 well low volume 5 mm Small 2 6 1536 well stand
63. le 10 14 Instrument catalog NUMDET 0 ccececeeeececeseeeeeteseeteeeees 109 Table 10 15 Codes for dispensing cassettes 00 0 ce ceeeeeeeeeeereeeeee 109 Table 10 16 Codes for accessories ecceeceeceescesesesestetesetesessnteeeteres 110 Table 10 17 Codes for WiAbC Si csctccavecatesssesstacaunscharasnestussueeabsavclacavedecnawecat 110 Table A 18 Channel and column settings for a 6 well plate 00 000000 115 Table A 19 Channel and column settings for a 12 well plate 0 0 0 0 116 Table A 20 Channel and column settings for a 24 well plate 0 0 0 0 117 Table A 21 Channel and column settings for a 48 well plate 0 0 118 Thermo Fisher Scientific Intended use Principle of Thermo Fisher Scientific operation Chapter 1 Introduction to the Multidrop Combi The available models of the Multidrop Combi are e 5840300 Multidrop Combi 100 240 V 50 60 Hz e 5840310 Multidrop Combi with SMART option 100 240 V 50 60 Hz Each Multidrop Combi comes with a sample of different dispensing cassettes including both small and standard tube cassettes The included cassettes can cover the whole volume area which then immediately enables full use of the instrument The Multidrop Combi microplate dispenser is intended for professional research use by trained personnel The instrument is intended for automated dispensing of up to eight different reagents simultaneously into a variety of micropl
64. les gt 50 pm in the reagent to avoid blockage of the tips especially when working with small tube cassettes Q13 What materials come into contact with liquids to be dispensed A13 The Small tube plastic tip dispensing cassette Cat no 24073290 and the Standard tube dispensing cassette Cat no 24072670 are made of PEEK silicone and PP polypropylene The Small tube metal tip dispensing cassette Cat no 24073295 is made of PEEK silicone ruby and stainless steel Q14 What should you do if the liquid foams during dispensing A14 Some liquids foam more easily than others One option is to reduce the pump rotor speed There are three dispensing speeds available High Med medium and Low 100 Thermo Scientific Multidrop Combi User Manual Thermo Fisher Scientific Thermo Fisher Scientific Frequently Asked Questions Q amp AS Q15 Do you need specific plate adapters for the Multidrop Combi A15 No the Multidrop Combi has a fixed plate adapter and does not use any external plate adapters Q16 What is the material of the Multidrop Combi priming vessel A16 The Multidrop Combi priming vessel Cat no N05640 is made of blue polypropylene PP QIZ Can the Multidrop Combi priming vessel be autoclaved A17 Yes it can be autoclaved Q18 Where can you see the Multidrop Combi internal software version number A18 When the instrument is powered on the version number only appears on the bottom of th
65. lied with the unit is always used The mains plug should only be inserted into a socket outlet provided with a protective ground contact Warning There are no user replaceable parts inside the instrument The user should not remove the cover from the Multidrop Combi a The same precautions applicable when using any electrical equipment should be observed with this instrument Do not touch switches or electrical outlets with wet hands Switch the instrument off before disconnecting it from the mains supply The dispensing cassette is a free moving mechanical device controlled by the instrument and not directly by the operator It is designed for hands off operation and should be used as such Never reach into the work space while the instrument is performing a dispensing cycle If it is necessary to stop the operation of the equipment press the STOP button on the control panel Thermo Scientific Multidrop Combi User Manual 105 Troubleshooting Guide Service request protocol Environmental Defects and abnormal stresses Service request protocol 106 Thermo Scientific Multidrop Combi User Manual Infectious samples and corrosive fluids are commonly used with this equipment The hands off nature of the system allows the user to dispense into the reaction wells without getting into direct contact with these fluids However the wells that have been in contact with potentially hazardous fluids must be handled with utmost
66. livery after each adjustment using steps 8 to 10 Three acceptable calibrations must be obtained Thermo Scientific Multidrop Combi User Manual 89 Calibration of the Dispensing Cassette Verifying and recalibrating the dispensing cassette 90 Thermo Scientific Multidrop Combi User Manual Figure 6 50 Screwing a calibration screw according to the calibration results 14 Continue in the same way with each channel until all eight channels have been recalibrated and close the calibration screw cover 15 Continue to set the calibration date Select Calibration gt Gravimetric gt OK _ Options alue qp gt Select OK Accept Use the Left and Right arrow keys to select the day month or year Use the Up and Down arrow keys to enter the real time calibration date Press OK Now the cassette contains the new calibration date the Since last calibration shows 0 but the Total usage remains unchanged and is always summed up Thermo Fisher Scientific Calibration standard tube Thermo Fisher Scientific volumetric Calibration of the Dispensing Cassette Verifying and recalibrating the dispensing cassette For volumetric calibration use the following steps pd Insert the dispensing cassette into the working position so that the lower and upper parts of the dispensing cassette are inserted into their slots Figure 4 14 2 Install the tube of the calibration device into the tip of the cha
67. ltidrop Combi User Manual 29 Routine Operation Dispensing cassettes Figure 4 18 Cassette with the tip and tubing weight protections removed 9 Place the tubing weight into the reagent vessel and always ensure that there is sufficient liquid present to run the protocol During prolonged standby keep the cassette in its rest position Figure 4 19 Figure 4 19 Rest position of the dispensing cassette 10 Pull the rotor cover over the rotor Figure 4 20 A sensor controls that the cover is properly placed over the pump rotor If the instrument is equipped with the SMART option and a SMART cassette is being used the calibration data window appears and the calibration data can be viewed The Calibration data view remains visible until you deactivate the view by pressing the OK or STOP button The Multidrop Combi is now ready for priming 30 Thermo Scientific Multidrop Combi User Manual Thermo Fisher Scientific Routine Operation Dispensing cassettes Figure 4 20 Pulling the rotor cover over the rotor Thermo Fisher Scientific Thermo Scientific Multidrop Combi User Manual 31 Routine Operation Control panel Control panel Keyboard Keys 32 Thermo Scientific Multidrop Combi User Manual This section describes the Multidrop Combi control panel and internal software The keyboard is shown in Figure 4 21 Control buttons Arrow keys and OK button Figure 4 21 Keyboard of the M
68. ltidrop Combi to dispense the first Ponceau S volume Select the 96 standard 15mm plate Press the PRIME button to predispense the tubes Insert the first empty 96 well plate Microplate A into the plate carrier Dispense the first volume into each well of the plate Empty the tubing of the first stock solution and insert all eight channel tubes into the second Ponceau S stock solution vessel Set the Multidrop Combi to dispense the second Ponceau S volume Press the PRIME button to predispense the tubes Insert the second empty 96 well plate Microplate B into the plate carrier Dispense the second volume into each well of the plate Table 6 11 Predispense the Multidrop Combi with Tween 0 02 v v Dispense the Tween solution into each well of both microplates Use the Low dispensing speed in this step to reduce foaming Dispense the appropriate volume according to Table 6 10 Note that the maximum volume with the small tube cassette is 50 pl You must dispense appropriate aliquots several times If you use the standard tube cassette you can dispense the whole volume in one go Shake the plate 15 seconds before measurement in the microplate reader or Multidrop Combi Measure Abses the absorbance of each well at 540 nm in both microplates using a microplate photometer with a measurement range of 0 to 2 Abs and a resolution of 0 001 Abs Attach a table of the Abses values to Table 6 11 Calculate the average absorbance
69. m this manual p 83 Install the dispensing cassette see Installing the cassette on page 27 Pull the rotor cover over the rotor Ensure that the priming vessel is installed Set the Multidrop Combi parameters as follows see Dispensing parameters on page 38 Plate type 96 standard 15mm Dispensing volume and cassette 10 pl small tube cassette 100 pl standard tube cassette Selected columns 1 10 Mark and weigh each strip separately beforehand Tare column Use disposable gloves when handling and weighing the strips Refer to Table 6 9 Place the 96 well plate with the 8 x 12 well microstrips into the plate carrier of the Multidrop Combi Use high purity water Press the PRIME button until all eight channels are dispensing continuously into the priming vessel Press the START button to start the first dispensing Wait until all 10 columns have been dispensed Repeat this step until dispensing has taken place 5 times in all with the small tube cassette final volume per well 50 pl or once with the standard tube cassette final volume per well 100 ul Weigh each dispensed strip Gross column immediately after dispensing to avoid evaporation The water that you use in the gravimetric method may be subject to evaporation depending on the environmental conditions during accuracy verification Refer to Table 6 7 Thermo Fisher Scientific Accuracy verification Thermo Fisher Scientific
70. mbi User Manual 27 Routine Operation Dispensing cassettes Figure 4 13 Inserting the upper part of the dispensing cassette pulling the tubes too much as excessive tension may damage the tubes or change the calibration of the dispensing cassette A Note The tension limiting wires are designed to prevent the user from 4 Double check that both the upper and the lower parts of the dispensing cassette are properly placed into their corresponding slots Figure 4 13 and Figure 4 14 Upper part slots Figure 4 14 Lower and upper parts of the dispensing cassette Inserted evenly into their slots 5 Ensure that all the tubes are evenly placed on the rotor needles Figure 4 15 four tubes on each half of the pump rotor 28 Thermo Scientific Multidrop Combi User Manual Thermo Fisher Scientific Thermo Fisher Scientific Routine Operation Dispensing cassettes Figure 4 15 Correct placement of all the dispensing cassette tubes 6 Ensure that the tension limiting wires have a loose fit around the rotor shaft 7 With small tube cassettes pull the tip protection off from the dispensing cassette once the dispensing cassette has been installed Figure 4 16 8 Remove the tubing weight protection by removing the rubber holder and pulling the tubing weight out of the tubing protection tube Figure 4 17 and Figure 4 18 Figure 4 17 Removing the tubing weight protection and rubber holder Thermo Scientific Mu
71. ng take back of the above mentioned materials The proposals for the procedures vary by country A Warning The dispensing cassette may contain biohazardous materials depending on the use of the cassette It consists of PEEK silicone stainless steel PA polyamide and PP polypropylene a 80 Thermo Scientific Multidrop Combi User Manual Thermo Fisher Scientific Verifying and recalibrating the dispensing cassette Accuracy verification gravimetric Materials and equipment needed for accuracy Thermo Fisher Scientific verification Chapter 6 Calibration of the Dispensing Cassette The verification and recalibration instructions cover the following three sections using the methods mentioned accuracy verification gravimetric precision verification photometric and calibration gravimetric The dispensing cassette has been calibrated by the manufacturer with deionized distilled water at 22 C 2 C It is recommended to recalibrate the dispensing cassettes at least monthly depending on the frequency of use and the liquids used It is recommended that you recalibrate the dispensing cassette if you forget the cassette in the Multidrop Combi under tension for prolonged periods for example a weekend or longer You should also check the calibration when liquid types other than water are used For accuracy verification use the example table Table 6 8 as a model when you fill out the results in the enclosed empt
72. ng the Multidrop Combi to reduce the risk of injury biohazardous contamination fire or electrical shock Read this user manual in its entirety prior to operating the instrument Failure to read understand and follow the instructions in the manual may result in damage to the instrument injury to laboratory and operating personnel or poor instrument performance Observe all Warning Caution and Note statements as well as safety symbols and markings on the instrument and in the documentation The Multidrop Combi is intended for laboratory research use only Observe proper laboratory safety precautions such as wearing protective clothing and following approved laboratory safety procedures Use of the Multidrop Combi in ways other than those described in the documentation supplied with the equipment may result in injury to persons or damage to the property Avoid unintended use of the equipment for example using incompatible materials making unauthorized modifications using incompatible or damaged parts using unapproved auxiliary equipment or accessories or operating equipment in excess of maximum ratings Preventative maintenance instructions should be followed closely to keep the instrument in the best condition for maximum reliability A poorly maintained instrument will not give the best results Thermo Fisher Scientific Chapter 1 Chapter 2 Chapter 3 Chapter 4 Thermo Fisher Scientific Conten
73. nnel to be recalibrated Figure 6 51 for example A A Figure 6 51 Placing the tube onto the tip of channel A 3 Pull the rotor cover over the rotor Press the OK or STOP button to deactivate the calibration data window 4 Place the tube of channel A into the reservoir with water at room temperature working temperature 5 Open the calibration screw cover on the upper part of the cassette Figure 6 53 6 Select Options gt Calibration gt Std tube volumetric Thermo Scientific Multidrop Combi User Manual 91 Calibration of the Dispensing Cassette Verifying and recalibrating the dispensing cassette i peee a Pat fee La E 7 Press the PRIME button until the water enters the calibration pipette 8 Adjust the water level in the pipette to the lower mark 0 9 by pulling or pushing the syringe piston Figure 6 52 Upper mark Figure 6 52 Adjusting the liquid level first to the lower mark and then to the upper mark should be at the upper mark 0 1 1000 pl 1 5 9 Press the START button and wait until the water level stops It Figure 6 52 10 If the volume is higher than 1000 ul turn the calibration screw Figure 6 53 clockwise with a screwdriver one turn for each 1 pl 1 from the volume dispensed if the volume is lower than 1000 ul turn in the opposite direction 92 Thermo Scientific Multidrop Combi User Manual Thermo Fisher Scientific Thermo Fisher Scie
74. ntific Calibration of the Dispensing Cassette Verifying and recalibrating the dispensing cassette Calibration screw cover opened Figure 6 53 Screwing the calibration screws according to the calibration results 11 Press the EMPTY and PRIME keys and the syringe to adjust the water level in the pipette to the lower mark and repeat steps 7 to 10 until the result is 1 ml Three accurate calibrations must be obtained Note The PRIME button must be pressed before START A 12 Change the tube to the next channel and repeat steps 7 to 11 13 Continue to set the calibration date Options easy en page ate dy aR ren ye Fata START to dispense 1000p STOP to cancel Aa tpt pr A ee Te el bind ees Bee rs Fal ed To Se t calibration date OK Accept Press OK to set the calibration date fora SMART cassette or to continue Thermo Scientific Multidrop Combi User Manual 93 Calibration of the Dispensing Cassette Verifying and recalibrating the dispensing cassette re F A SS opion Set calibration date Value d4 Select OK Accept Use the Left and Right arrow keys to select the day month or year Use the Up and Down arrow keys to enter the real time calibration date Press OK 14 Close the calibration screw cover Now the cassette contains the new calibration date the Since last calibration shows 0 but the Total usage remains unchanged and is always summed up Main Settings
75. o Fisher Scientific technical service department Check Packing for service on page 68 You will find instructions on how to proceed before shipping the instrument for service to Thermo Fisher Scientific Oy Thermo Fisher Scientific Certificate of Decontamination Thermo Fisher Scientific Troubleshooting Guide Certificate of Decontamination Check that any necessary decontamination procedure has been carried out before packing Refer to Decontamination procedure on page 66 and Certificate of Decontamination on page 107 Ensure that the Certificate of Decontamination see Appendix B Certificate of Decontamination as well as the return authorization number RGA are sent with the instrument The Thermo Fisher Scientific technical service department will keep you up to date with the progress of service and provide you with any further details you might need for example on maintenance serviceability troubleshooting and replacement The decontamination procedure is required before shipping the instrument to Thermo Fisher Scientific Oy for example for repair If for any reason the instrument is shipped back to Thermo Fisher Scientific Oy it must be accompanied by a dated and signed Certificate of Decontamination which must be attached to the outside of the package containing the instrument Refer to Appendix B Certificate of Decontamination and Decontamination procedure on page 66 Fail
76. o the reservoir 2 If there is only a break of a few minutes in dispensing no washing or cleaning of the tubes is necessary However after pressing the EMPTY button release the tubing tension by pulling the upper part of the dispensing cassette from its slots and place it into its rest position slots located on the left hand side of the rotor Figure 4 19 Thermo Fisher Scientific Routine Operation Emergency situations 3 If there is a longer break for example a day wash the dispensing cassette by priming it with deionized distilled water or with special washing detergent if necessary 4 After washing and emptying store the dispensing cassette in the rest position Figure 4 19 5 Switch the Multidrop Combi off by pressing the mains switch Figure 2 4 at the left of the back panel of the instrument into the OFF position 6 Wipe the instrument surfaces with a soft cloth or tissue paper moistened with deionized distilled water a mild detergent SDS sodium dodecyl sulfate or soap solution 7 Ifyou have spilt infectious agents on the dispenser disinfect with 70 alcohol or some other disinfectant see Decontamination procedure on page 66 dispensing cassette is not in use Figure 4 19 A Note Keep the dispensing cassette in the rest position when the Emergency In case there is any abnormal situation during operation such as fluids spilling inside the instrument follow these steps situations P
77. ode printed on it or the tag can simply be mounted inside a carton or embedded in plastic See antenna and RFID Thermo Scientific Multidrop Combi User Manual 123 Glossary tension limiting wire tension limiting wires The tension limiting wires Figure 4 10 and Figure 5 32 are designed to prevent the user from pulling the tubes too much as excessive tension may damage the tubes or change the calibration of the dispensing Cassette USB Universal serial bus 124 Thermo Scientific Multidrop Combi User Manual Thermo Fisher Scientific Glossary Thermo Fisher Scientific Thermo Scientific Multidrop Combi User Manual 125 Glossary 126 Thermo Scientific Multidrop Combi User Manual Thermo Fisher Scientific Index 1 1536 well plates 13 14 50 62 3 384 well plates 13 14 49 61 62 110 6 6 48 well plate dispensing instructions 113 9 96 well plates 14 49 62 81 82 85 86 87 100 110 A accessories 6 19 20 110 accuracy verification 81 82 83 84 87 advantages of using Multidrop Combi 15 alibration 60 antenna 61 123 autoclaving the dispensing cassette 71 B backflush 15 32 123 C calibration 28 30 35 60 61 73 76 77 78 79 81 83 87 88 89 90 91 92 93 94 99 100 101 123 124 date 90 93 94 screw 73 76 77 78 79 89 90 91 92 93 94 123 CE marking 123 cleaning the tips 70 column selection 13 35 41 42 command set 57 computer inter
78. of the dispensing cassettes of the Multidrop Combi with 40 cm tubing AG The dead volume is about 1 0 ml with the small tube cassettes However the dead volume is lt 7 ml for the standard tube cassette Q7 Does the dispensing cassette of the Multidrop Combi include a calibration certificate A7 Yes all dispensing cassettes include a factory calibration report Thermo Scientific Multidrop Combi User Manual 99 Frequently Asked Questions Q amp As Q8 What liquid is recommended to be used for washing the Multidrop Combi tubes A8 It is recommended that you use deionized distilled water and or detergent solutions for example 0 2 1 Tween followed by deionized distilled water Q9 When do you have to calibrate the dispensing cassettes of the Multidrop Combi A9 It is recommended that you recalibrate at least monthly depending on the frequency of use and the liquids used Q10 Can the dispensing cassettes of the Multidrop Combi be recalibrated A10 Yes they can QII What is the tip inner diameter of the dispensing cassettes of the Multidrop Combi A11 The tip inner diameter is either 0 22 mm Small tube plastic tip dispensing cassette Cat no 24073290 and Small tube metal tip dispensing cassette Cat no 24073295 or 0 5 mm Standard tube dispensing cassette Cat no 24072670 Q12 Do particles in the reagent affect dispensing A12 Possibly It is recommended that you ensure that there are no partic
79. op Combi Principle of operation 14 Thermo Scientific Multidrop Combi User Manual The Multidrop Combi uses detachable and autoclavable dispensing cassettes The dispensing cassette contains eight individual tubes Each reagent can have a separate dispensing cassette to avoid mixing of reagents Alternatively the same dispensing cassette can be used with several reagents if the dispensing cassette is washed in between use Refer to Dispensing cassettes on page 25 All eight reagent lines can be backflushed to the reagent bottle to minimize the loss of expensive reagents Figure 1 1 Multidrop Combi microplate dispenser With the 96 well plate selection the columns with 8 wells are filled starting from column 1 according to the selected columns after which the plate carrier returns to the home position and the pump lifting mechanism to the up position Row wise and column wise dispensing show no differences in 96 well plate dispensing With the 384 well plate selection the columns with 16 wells are filled in two phases first every other row A C E G O starting from the first column or the columns selected and then the pump carrier shifts sideways so that the remaining rows B D F H P are filled in the opposite order If column wise dispensing is used it shifts the pump carrier first sideways filling 16 wells and then the plate carrier moves dispensing over the next column With the 1536 well plate selection
80. or the actual end user for example laboratory technician and provides information on the Thermo Scientific Multidrop Combi including the installation and operating instructions How to use this This user manual is designed to give you the information to user manual o Review safety precautions e Setup the Multidrop Combi e Use the Multidrop Combi internal software e Carry out dispensing procedures e Perform basic cleaning and maintenance procedures e Troubleshoot the instrument performance This user manual also describes all the features and specifications of the Multidrop Combi instrument as well as ordering information Read the manual in its entirety before operating the instrument Keep the user manual for future reference The user manual is an important part of the instrument and should be readily available during use of the instrument Keep the user manual together with the instrument in case you distribute it onwards For more For the latest information on products and services visit our websites at information http www thermo com http www thermo com multidrop In an effort to produce useful and appropriate documentation we appreciate your comments on this user manual to your local Thermo Fisher Scientific representative Thermo Fisher Scientific Thermo Scientific Multidrop Combi User Manual 3 About This User Manual safety symbols and markings Safety symbols These symbols are intended to draw your a
81. otes Thermo Fisher Scientific Thermo Scientific Multidrop Combi User Manual 131 Notes 132 Thermo Scientific Multidrop Combi User Manual Thermo Fisher Scientific Notes Thermo Fisher Scientific Thermo Scientific Multidrop Combi User Manual 133 Thermo Fisher Scientific Oy Microplate Instrumentation Ratastie 2 RO Box 100 Fl 01621 Vantaa Finland www thermo com N05616
82. ow key continuously The minimum and maximum dispensing volumes are displayed in the Volume and cassette window Refer to Table 4 4 below Table 4 4 Dispensing volumes of the cassettes Cassette Volume range pl Increment pl Small tube cassette 0 5 50 0 5 Standard tube cassette 5 2500 5 40 Thermo Scientific Multidrop Combi User Manual Thermo Fisher Scientific Routine Operation Dispensing parameters The default cassette types and dispensing volumes for different plate types are given in Table 4 3 ok Accept the selections using the OK button Ensure that the cassette selection corresponds to the installed cassette Column selection You can dispense either into the whole plate or only into certain selected columns Go to the Main menu Select the Column selection row O 384 standard 15mm oA O 5 0pl Small cassette Full plate Select OK Accept If you want to dispense into all the wells of the plate select Full plate if it is not already shown on the screen If you want to dispense only into some of the wells select Selected columns using the Down arrow key Thermo Fisher Scientific Thermo Scientific Multidrop Combi User Manual 41 Routine Operation Dispensing parameters Full plate Ww y n aleli ielien Column number E Pia is Pie Pr Pie 19 m ai A a 4b Move OK Fill Empty CE E oS Use the arrow keys to move from one column to the next 42
83. parameters are set in the Settings menu The values shown in the Settings menu are those set for the plate type that is selected in the Main menu Note The protocol settings do not normally have to be changed in routine use A Note An exclamation mark will appear in the menu tab and in the circle on the main view row in question when the setting differs from the default parameter setting A Settingso Speed MED Disp offset 0 0 0 0mm Disp height 16 00mm Predispense 2ul Direction OK Pump speed The changes made to the protocol settings only remain in the instrument memory if the protocol is saved with a name see Save and edit protocol on page 51 If the protocol is not saved the changes only remain effective until the plate type is changed or the instrument is switched off Thermo Fisher Scientific Routine Operation Protocol settings Dispensing speed The default dispensing speed is High but you can change the Thermo Fisher Scientific dispensing speed for example to reduce foaming Note that the er Medium dispensing speed gives the best dispensing results with most of the buffers Go to the Settings menu Select the Pump speed row any Settings Woptions Us Disp offset 0 0 0 0mm UJ Disp height 45 00mm Main Settings WOptions 5peed OK Accept es E gt Use the Up and Down arrow keys to select the pump speed The available settings are High Med medium
84. pcs box Microfluor 1 black plate flat bottom 50 pcs box Black 96 well plate UB flat bottom 50 pcs box C12 Maxisorp Nunc lmmuno Module 60 pcs box Microtiter 96 deep well plate 50 pcs box 95040450 95040000 95040020 110 Thermo Scientific Multidrop Combi User Manual 384 well microplates 384 round well plate 50 pcs box White 384 round well plate 50 pcs box Thermo Fisher Scientific Thermo Fisher Scientific Literature Chapter 11 References Editorial 2004 Multidrop bulk reagent dispenser family from Thermo Electron precise dispensing in the microliter area allowing assay miniaturization Innovations in Pharmaceutical Technology 4 14 36 Falconer M et al 2002 High Throughput Screening for Ion Channel Modulators Journal of Biomolecular Screening 7 5 460 465 Kiger A A et al 2003 A functional genomic analysis of cell morphology using RNA interference Journal of Biology 2 27 Tanaka M et al 2005 An unbiased cell morphology based screen for new biologically active small molecules PLoS Biology 3 5 e128 Yarrow J C et al 2004 A high throughput cell migration assay using scratch wound healing a comparison of image based readout methods BMC Biotechnology 4 21 Thermo Scientific Multidrop Combi User Manual 111 References 112 Thermo Scientific Multidrop Combi User Manual Thermo Fisher Scientific Thermo Fisher Scientific Appendix A 6 48 Well Plate Dispensing In
85. r from live parts and they should only be removed after switching the instrument off and disconnecting the mains supply cable and only by suitably qualified maintenance and repair personnel Warning The instrument uses voltages dangerous for human beings Before removing any covers disconnect the instrument from the power supply 4 Multidrop Combi bears the following markings Type 836 100 240 Vac 50 60 Hz 100 VA CE mark CSA monogram Thermo Fisher Scientific Thermo Fisher Scientific Technical Specifications In conformity with the requirements Multidrop Combi conforms to the following requirements 2006 95 EC Low Voltage Directive 2004 108 EC Electromagnetic Compatibility Directive EMC FCC Part 15 Subpart B Class B July 2004 2002 96 EC Waste of Electrical and Electronic Equipment Safety performance EN 61010 1 2001 Ed 2 taking into account US and CA National differences The safety specifications are also met under the following environmental conditions in addition to or in excess of those stated in the operating conditions Altitude Temperature Humidity Mains supply fluctuations EMC performance EN 61000 6 3 2001 EN 61000 6 1 2001 EN 61326 1 1997 A1 1998 A2 2001 A3 2003 Up to 2000 m 5 C to 40 C Maximum relative humidity 80 for temperatures up to 31 C decreasing linearly to 50 relative humidity at 40 C 10 from nominal Generic standards Emission s
86. rd and display 18 32 95 maintenance 3 6 65 68 69 96 107 markings 123 N navigating 32 33 34 37 0 operational check 23 P packing instructions 19 68 packing list 19 plate 110 carrier 14 22 23 25 46 49 50 68 82 86 103 123 type 35 36 37 38 39 40 41 44 46 47 48 49 50 54 58 59 82 88 113 precalibration 78 79 precautions and limitations 20 precision verification 81 83 85 predispense 23 32 37 48 69 86 123 128 Thermo Scientific Multidrop Combi User Manual predispensing 35 48 67 69 103 123 volume 35 48 prime 23 32 35 36 37 48 50 67 69 70 82 86 89 92 93 95 103 104 113 123 priming vessel 15 23 25 36 50 65 66 68 82 85 88 89 95 101 103 104 110 113 priming volume 35 48 principle of operation 13 15 protocol settings 44 pump 13 14 22 23 27 28 30 32 45 46 47 49 50 61 101 103 123 lifting mechanism 14 23 49 103 rotor 23 27 28 29 30 31 36 61 62 82 85 88 91 101 103 104 113 speed 15 35 40 45 86 95 96 101 reagent pretreatment 26 recalibrating the dispensing cassette 81 releasing the transport lock 21 remote control 15 57 98 123 remote mode 35 57 98 rest position 30 36 62 63 69 70 114 RFID 61 99 101 123 tag 21 22 61 99 101 123 robotic 14 rotor 23 27 28 29 30 31 36 61 62 82 85 88 91 101 103 104 113 cover 23 30 31
87. ress Sz 384 standard 15mm If you want to change the 5 0pl Small cassette volume press N 50 F Standard Sp Full plate select the appropriate anes abc Default protocol cassette with wr OK Volume and cassette Set the needed volume with Volume lt 4 gt Cassette OK Accept To accept press 3 Columns see Step 5 on p 36 If you intend to fill the A ea gine whole plate you can start Se standard 15mm dispensing after priming 5 0pl Small cassette the instrument Full plat cd ue If you intend to fill only part abc O Default protocol of the columns press OK Select columns and To select the columns navigate with and to confirm each selection press Last select Accept with When the Accept box is 4 gt Move OK Fill Empty highlighted press You are now ready to start Selected column s red after filling the tubes through predispense Thermo Fisher Scientific Thermo Scientific Multidrop Combi User Manual 37 Routine Operation Using internal software Using internal This section describes procedures related to the Multidrop Combi software internal software Multidrop Combi can also be controlled with PC software Thermo Scientific FILLit Software for Multidrop Combi Cat no 5188010 For more information refer to Thermo Scientific FILLit Software for Multidrop Combi User Manual Cat no N07173 Dispensi Ng This section describes the relevant d
88. s priming vessels disposable gloves syringes disposable tips and so on as biohazardous waste A Decontamination should be performed in accordance with normal laboratory procedures Any decontamination instructions provided with the reagents used should be followed A decontamination procedure is only recommendable when infectious substances have been in direct contact with any part s of the instrument If there is a risk of contamination with biohazardous material the procedure recommended below or some other corresponding decontamination procedure must be performed It is strongly recommended to perform the complete decontamination procedure before relocating the instrument from one laboratory to another Decontamination is not required for the proper functioning of the instrument Example of decontaminants e Ethanol 70 e Virkon solution 1 3 e Glutaraldehyde solution 4 e Chloramine T e Microcide SQ 1 64 Caution If local or laboratory regulations prescribe regular decontamination it is not advisable to use formaldehyde since even small traces of formaldehyde negatively affect the enzyme being used in EIA tests resulting in bad test results A Warning The decontamination procedure should be performed by authorized trained personnel in a well ventilated room wearing disposable gloves protective glasses and clothing A 1 Prepare the decontaminant for example 1 3 Virkon solution or 200 ml 4 glutaralde
89. se when _ Predispensing undone Press the PRIME button pump not primed 15 Missing prime vessel Priming vessel missing Insert the priming vessel 16 Rotor shield not in Rotor cover not in place Pull the rotor cover over the pump place Examples of a few error messages that appear in the Multidrop Combi internal software are shown below Thermo Fisher Scientific Thermo Scientific Multidrop Combi User Manual 103 Troubleshooting Guide Error and warning codes Main Settings Options Press OK T Main Settings Options Press OK Main Settings l Options Press OK Press OK to acknowledge the error and then pull the rotor cover over the rotor 104 Thermo Scientific Multidrop Combi User Manual Thermo Fisher Scientific Thermo Fisher Scientific OC Hazards Electrical A Mechanical Troubleshooting Guide Hazards An example of a warning message that appears in the main view of the Multidrop Combi internal software is shown below Dispensing volume exceeds well volume OK Continue anyway STOP Cancel Press OK to acknowledge the warning and continue dispensing or press STOP to cancel and then reselect the dispensing volume This instrument is designed to provide full user protection When correctly installed operated and maintained the instrument will present no hazards to the user The following recommendations are given to ascertain added user safety Ensure that the mains supply cable supp
90. sette into the rest position Figure 4 19 Refer to Shutdown on page 62 Handle the dispensing cassette with great care so that you do not damage the tubes and dispensing tips These factors affect the useful life of the dispensing cassette Caution With small tube cassettes ensure that the liquid or reagent does not contain any particles gt 50 pm and that the liquid container is covered Avoid dust or any particles gt 50 pm when operating with the dispensing cassette A The following instructions are valid for the Small tube plastic tip dispensing cassette and the Standard tube dispensing cassette If the tip s are clogged you can first try the following alternative procedures to remove the blockage pd Press the EMPTY button for a few seconds Then press the PRIME button for a few seconds 2 Place a liquid reservoir filled with deionized distilled water under the cassette tips so that the tips are submerged in liquid and press the EMPTY button 3 Clean the tips according to the procedure described below gray metal tip cassette only Use the cleaning tool provided Note To avoid particles re entering the reagent place the tubing end weight into a separate vessel while you empty the tubing A The following cleaning instructions are only valid for the Small tube metal tip dispensing cassette 1 Unfasten the four screws of the cover 2 Detach the tubing from the tips and remove the tip holder from the Small tube
91. sing is used it shifts the pump carrier first sideways filling 16 wells and then the plate carrier moves dispensing over the next column Thermo Scientific Multidrop Combi User Manual 49 Routine Operation Running a protocol With the 1536 well plate selection the columns with 32 wells are filled in four phases first every fourth row A E I M AC and then the pump carrier shifts sideways above the rows B F J N AD which are then filled in the opposite order After three shifts the remaining rows D H L P AF are filled Column wise dispensing shifts the pump carrier sideways three times filling 32 wells and then the plate carrier moves the dispensing head over the next column In 384 and 1536 well plate mode row wise and column wise dispensing can be effectively used Running a Torun a dispensing protocol follow the steps below protocol Note Do not dispense extensively without any liquid e Check that there is always enough liquid in the reservoir and ensure that all the tube ends are below the liquid level Use the PEEK tubing weight supplied e Check after each dispensing that the priming vessel is not filled up A l Make sure you are in the Main menu 2 Select the plate type The protocols are listed according to each plate type 3 Select the dispensing protocol from the protocol list 4 Keep the PRIME button down until the tubings are completely filled 5 Press the START button to start t
92. ssasentar 106 Sevice TEQUESE Protocoles E tase et ame ancl 106 Certificate of Decontamination vscesuceveduseswacssegocutsnpedencsosnacvadnpeanmedss 107 Chapter 10 Ordering InformatiOn sescssseesessesssesssesesseseeseseeseseeseeatseesess 109 Wirt top COM Di scorie a aE 109 Pipen na CASSE LLCS en A 109 Biror CEOE aE E E E E RER 110 Liitot Plates irern n EREN E 110 Chapter 11 PROTON GING OS nonna N 111 ECA 2 Giese carne ce Sale e tet alana sales ae seme te 111 Appendix A 6 48 Well Plate Dispensing Instructions cccssseseeseeeees 113 Ge welll Plate e cage cate tice acpi E E 115 eal io eavoucloas beh Armen er ree eter eee ree nner nt rer ttre N 116 DA AWe ll PlALe SEU INS ucts cures a eer ahvaccns eerste ies 117 AS Well PALE SELENE S sci vin bets can in trey le ts eine aki vaya tena iptelieeinteiehe ae 118 Appendix B Certificate of Decontamination ccccssssssssesssesseesseseteeee 119 Appendix C Thermo Scientific Multidrop Combi Feedback Form 121 Thermo Fisher Scientific Thermo Scientific Multidrop Combi User Manual 9 Figures 10 Thermo Scientific Multidrop Combi User Manual Figures Figure 1 1 Multidrop Combi microplate dispenser 14 Figure 2 2 Multidrop Combi front VieW 0 cccceeecceececesesceseteteseeeereeen 17 Figure 2 3 Close up of the Multidrop Combi keyboard and display 18 Figure 2 4 Multidrop Combi back ViOW cece cce
93. ssettes are also available with long tube versions see Table 10 15 8 silicone tubings Tip protection Plastic covers Tension Imiting wires Tips Sight window a sight J Tubing weight Tubing weight protection Figure 4 10 Parts of a dispensing cassette 26 Thermo Scientific Multidrop Combi User Manual Thermo Fisher Scientific Thermo Fisher Scientific Installing the cassette Routine Operation Dispensing cassettes 1 Take the lower part of the cassette with the tip protection only in small tube cassettes into your right hand with the dispensing cassette tips pointing down and the upper part into your left hand Figure 4 11 Figure 4 11 Inserting the dispensing cassette 2 Carefully place the eight tubes below the pump rotor and insert the lower part of the dispensing cassette into the lower part slots of the pump body Figure 4 12 Check that the tubes are freely placed below the pump rotor and the tension limiting wires below the rotor shaft Lower part slots Figure 4 12 Inserting the lower part of the dispensing cassette into Its slots 3 Take a firm grip on the upper part of the dispensing cassette with your right hand and carefully pull the tubes around the pump rotor until the upper part reaches and fits into the upper part slots of the pump body Figure 4 13 Insert into the slots Hold onto the instrument firmly with your other hand Thermo Scientific Multidrop Co
94. structions To dispense into a 6 12 24 or 48 well plate leave some of the channels empty of liquid and select only some of the columns Insert the correct dispensing cassette and the priming vessel Close the rotor cover Make sure you are in the Main menu Select 96 as the plate type and insert the 6 12 24 or 48 well plate Remove the tubes for the channels you leave empty from the tubing weight manually Keep the removed tubes outside the reagent bottle while dispensing For information about the channel settings Figure 11 54 see the following sections below First select the cassette type and then the dispensing volume Select the columns For information about the column settings see the following sections below Keep the PRIME button down until the tubings are completely filled Thermo Scientific Multidrop Combi User Manual 113 6 48 Well Plate Dispensing Instructions 8 Gra Press the START button to start the protocol The instrument dispenses the plate Ds Press the EMPTY button to empty the dispensing tubing 10 man During prolonged standby keep the cassette in its rest position Remove the cassette when the maximum amount of plates are dispensed x or you want to change the cassette type in use j Make sure you wash the cassette properly l1 O Power OFF the instrument ATTENTION TUBINGS Rotating mechanism D C B A Close the roter cover before use O O O O Release the tubings 0 Oo
95. tandard for residential commercial and light industrial environments Generic standards Immunity standard for residential commercial and light industrial environments Product family standard Thermo Scientific Multidrop Combi User Manual 97 Technical Specifications Remote control to Multidrop Combi Remote control to Multidrop Combi 98 Thermo Scientific Multidrop Combi User Manual Test standards EN 55022 1998 A1 2000 A2 2003 EN 61000 3 2 2000 EN 61000 3 3 1995 A1 2001 ANSI C63 4 2003 EN 61000 4 2 1995 A1 1998 A2 2001 EN 61000 4 3 2002 A1 2002 EN 61000 4 4 2004 EN 61000 4 5 1995 A1 2001 EN 61000 4 6 1996 A1 2001 EN 61000 4 8 1993 A1 2001 EN 61000 4 11 1994 A1 2001 Performance limits Class B 150 kHz 1 GHz Class A Class B 150 kHz 30 MHz 30 MHz 1 GHz 4 kV CD 8 kV AD Criteria B 3 V m 80 MHz 2 GHz Criteria A 1 kV Criteria B 2 kV line to ground 1 kV line to line Criteria B 3 Vims 190 kHz 80 MHz Criteria A 30 A m Criteria A 30 10 ms Criteria B 60 100 ms Criteria B 100 10 ms Criteria B 100 5 s Criteria C The Multidrop Combi can be used as part of a robot system When the Multidrop Combi is in remote mode the main view is as shown below For more details refer to the Thermo Scientific Multidrop Combi Remote Control Command Sets document Cat no D04640 Contact your distributor or local Thermo Fisher Scientific representative to obt
96. tep calibration screws and the PEEK tubing weight from the previous tubing set Tubing identification plate with tube lot number Figure 5 28 New tubing set with the tip band attached 2 Remove the four cover retaining screws on the lower part of the dispensing cassette the tubes with the tube fasteners and the tip manifold with the tips the three cover retaining screws on the upper part of the dispensing cassette the two tension limiting wires the calibration screws Figure 5 43 and the tubes with the tube fasteners Save the calibration screws and the PEEK tubing weight for installing the new tubing set 3 Insert the tip manifold so that the tips go through the holes in the lower part of the dispensing cassette Figure 5 29 Guide the tubes through the eight openings in the side wall by pressing the small cubic tube fasteners into place Figure 5 30 Ensure that each tube is in its designated opening Figure 5 31 y IW Tube fasteners Tip band Figure 5 29 Inserting the tip manifold into the lower part of the dispensing cassette Thermo Fisher Scientific Thermo Scientific Multidrop Combi User Manual 73 Maintenance Changing the tubing set Slots for tension limiting wires 74 Thermo Scientific Multidrop Combi User Manual Figure 5 31 New tubing Inserted into the lower part of the dispensing cassette 4 Attach the tension limiting wires into their slots Figure 5 32 Figure 5 32 T
97. tific Multidrop Combi User Manual 119 Certificate of Decontamination 120 Thermo Scientific Multidrop Combi User Manual Thermo Fisher Scientific Appendix C Thermo Scientific Multidrop Combi Feedback Form Instrument Thermo Scientific Multidrop Combi O Instrument serial no PURCHASED BY Company Institute Department Address Tel Fax PURCHASED FROM Distributor Address Tel Date of delivery Internet home page Date of purchase Your application area D O M O Mrs 0OO Ms 0O Job title Position Surname block capitals First name block capitals Internet e mail address Excellent Above expectations Instrument installation o O Ease of use oO O Flexibility oO oO User manual oO oO software o O Customer support oO o Overall O oO Additional instrument and or software features desired Did you encounter any problems As expected Below expectations Comments O O OOoOddadda OOOdddQ Where did you first learn about the product Would you like to receive information about other Thermo Scientific products Thermo Fisher Scientific Thermo Scientific Multidrop Combi User Manual 121 Thermo Scientific Multidrop Combi Feedback Form 122 Thermo Scientific Multidrop Combi User Manual Thermo Fisher Scientific Glossary antenna The tag antenna is the conductive element that enables the tag to send and receive data The RF energy from the
98. ts ende dii i erea A EEA 3 How touse this user mantal srsicaninpneiarn e a 3 For more morma oN e E 3 Safe symbolsand Markings iisen E E 4 Safety symbols and markings used on the Multidrop Combi 4 Warning and other markings used in the documentation 5 Instrument safety and guidelines for use sssssesesseseseessessersserssseses 6 Introduction to the Multidrop Combi cccccssssssesssesesesseeseees 13 PACING Wesnes Vet bled aan a a ead element 13 IPEMICIP le Ol operation senie r E mies aetheeien a 13 Advantages of using Multidrop Combi 0 eee eeeeeeeeseeeeeneeeeeeeees 15 Functional Description cccssssssssssssessssssesssssrsesssereesssstersessseraesesens 17 Riser arent ayota ara N N I7 POMC VICW sa a E A 17 BE AAEE E L E E E E A E E A 18 MStalati oN annn ee at tes 19 Deliver ONEC k a a E S 19 Ceckine deli very enoo ara E T 19 Uapack Menea a a A re ere 19 FNC QUITO ICIS raa sta uanrto eee ones beaten Neato 20 Precautions And MAMLATIONS renn E E 20 Mistal tions ctp ea aE E 21 Releasing thet ransport JOCK sic sa carnconicsarinemaniaieneneiae 21 Fastening thet rans port lock agers a ssnda sc gesdacasissoescgundoes sce peeuwedepuecenes 22 Operational checkar a A 23 Routine OO rat OM sssusa eeeearaaeR 25 Primine Vessel cesahitstsccsteescetetccaap a a eas eee 25 NES PEMSUMN ES CASSE OS ae E T T T 25 Trista live the Cassel 6 neien na a parse tauraaneennaeaiee 2 COOL r a A E EA 32 Wey
99. ttention to particularly important information and alert you to the presence of hazards as and markings F i indicated Safety symbols and The following symbols and markings appear on the type label and the y sy g sy gs app yp markings used on instrument itself the Multidrop Combi Power ON a Power OFF a Serial number a Catalog number a Date of manufacture a Consult instructions for use a WEEE symbol This product is required to comply with the European Union s Waste Electrical amp Electronic Equipment WEEE Directive 2002 96 EC A Bc HHO 4 Thermo Scientific Multidrop Combi User Manual Thermo Fisher Scientific Warning and other markings used in the documentation Thermo Fisher Scientific DEBE About This User Manual safety symbols and markings The following symbols and markings appear in this user manual Warning Risk of electric shock A Warning Biohazard risk A Warning Risk of injury to the user s A Caution Risk of damage to the instrument other equipment or loss of performance or function in a specific application A Note Marks a hint important information that is useful in the optimum operation of the system or an item of interest A Thermo Scientific Multidrop Combi User Manual 5 About This User Manual Instrument safety and guidelines for use Instrument safety and guidelines for use 6 Thermo Scientific Multidrop Combi User Manual Always follow basic safety precautions when usi
100. ttings and Options In routine use you mainly navigate in the Main menu In advanced level options there are two extra levels Settings and Options You can navigate between these three menu levels using the Left and Right arrow keys The main view row is either colored active or uncolored inactive All the descriptive icons used in the main view are shown in Table 4 2 below The info text bar shows explanatory information on how to proceed and which keys to use Thermo Scientific Multidrop Combi User Manual 33 Routine Operation Control panel Navigating Active item abc Default protocol OK Plate type 34 Thermo Scientific Multidrop Combi User Manual O 384 standard 15mm Tee 5 0pl Small cassette O Full plate Default protocol OK Volume and cassette This section visualizes navigation in the Multidrop Combi user interface The main view changes according to the selections you make either with the Up and Down arrow keys or the OK button The available buttons and their function are shown on the info text bar 4 Standard E tube cassette tube O Available buttons and their function cassette Volume 4p gt Cassette OK Accept The color of the items for example the icon and main view row in the main view changes when they are selected active inactive To move from one menu to another make sure you are in the main view of one of t
101. ultidrop Combi The relevant keys and control buttons are described in detail below The arrow keys are used to select modify and change the parameters and to navigate in the main view The OK button is used to accept the selection The START button is used to start the dispensing protocol The STOP button is used to stop the protocol at any time The plate and pump body are returned to the home position While editing values the changes can be discarded by pressing the STOP button The STOP button is also used to stop the shaking action Note In addition the STOP button is used as an Esc Escape Cancel button A The PRIME button is used to fill the tubings of the cassette and predispense Predispenses as long as you press the button The EMPTY button is used to empty the liquid from the dispensing cassette into the liquid reservoir backflush option Empties as long as you press the button The SHAKE button is used to shake the plate linearly The shaking is started by pressing the SHAKE button and stopped by pressing the STOP button Thermo Fisher Scientific Thermo Fisher Scientific Display Icon Routine Operation Control panel The main view in the display is shown in Figure 4 22 Main 1 Menu tabs Main view row OK Plate type Info text bar Figure 4 22 Main view on the display of the Multidrop Combi There are three menu tabs in the Multidrop Combi user interface Main Se
102. ure to confirm decontamination will incur additional labor charges or at worst the items will be returned for proper cleaning Before returning any instrument s or item s ensure that they are fully decontaminated Confirm A or B status Refer to Appendix B Certificate of Decontamination Thermo Scientific Multidrop Combi User Manual 107 Troubleshooting Guide Certificate of Decontamination 108 Thermo Scientific Multidrop Combi User Manual Thermo Fisher Scientific Chapter 10 Ordering Information Contact your local Thermo Fisher Scientific representative for ordering and service information Table 10 14 Instrument catalog number Multidrop Comb Code Instrument System 5840300 Multidrop Combi 100 240 V 50 60 Hz 5840310 Multidrop Combi with SMART option 100 240 V 50 60 Hz Dispensing Table 10 15 Codes for dispensing cassettes Cassettes Code Item 24073290 Small tube plastic tip dispensing cassette 24073291 Small tube plastic tips dispensing cassette pack of 5 24073292 SMART small tube plastic tip dispensing cassette 24073295 Small tube metal tip dispensing cassette 24073296 Small tube metal tip dispensing cassette pack of 5 24073297 SMART small tube metal tip dispensing cassette 24073001 SMART small tube plastic tip dispensing cassette b pack 24073002 SMART Small tube metal tip dispensing cassette 5 pack 24072670 Standard tube dispensing cassette 24072671 Standard tube dispensing cassette 5 pack 24072672
103. wed ok Press OK 60 Thermo Scientific Multidrop Combi User Manual Thermo Fisher Scientific Dispensing cassette with Thermo Fisher Scientific SMART option Routine Operation Instrument options The SMART dispensing cassette has an RFID tag incorporated that contains the information when the cassette was last calibrated the cassette type how much the cassette has been used for dispensing and the total usage Refer to antenna RFID and RFID tag in the glossary The Multidrop Combi equipped with SMART option calculates the usage of the cassette by incrementing rotations of the pump The number of rotations is then compared to the specified lifetime value of each cassette You can check the SMART cassette calibration data either by pulling the rotor cover over the rotor or by selecting Options gt QC cassette values gt OK For instance the small tube cassette lifetime specification is 1000 plates of 384 wells 5 ul This equals 60 000 rotations of the peristaltic pump One 384 well plate 5 ul equals 60 rotations which is 0 1 of the total usage The Since last calibration percentage value will turn to zero every time the cassette is calibrated The Total usage value will follow the lifetime of the cassette Refer to the Calibration data display below If the total usage exceeds 100 that is is in the red the cassette in question should be changed to ensure secure dispensing performance However the cassette can
104. y table Table 6 9 in the accuracy verification worksheet The volume conversion factor Z for water to change the weights into volumes at different temperatures is presented in Table 6 7 The gravimetric method of accuracy verification requires you to weigh each individual strip that contains water to be dispensed from the Multidrop Combi The analytical balance that you use should be well maintained calibrated with an appropriate set of certified weights and capable of reading with a resolution of at least 0 1 mg preferably 0 01 mg The gravimetric method also requires a thermometer that should be calibrated and readable to 0 5 C The following materials and equipment are required for accuracy verification Multidrop Combi with the dispensing cassette to be verified inserted e Analytical balance resolution at least 0 1 mg preferably 0 01 mg e Calibrated thermometer readable to 0 5 C e 96 well plate with 8 x 12 well microstrips for example Thermo Scientific Microtiter 1 x 12 Strip Assembled Universal Binding Flat Cat no 95029370 or another similar 8 x 12 strip plate Thermo Scientific Multidrop Combi User Manual 81 Calibration of the Dispensing Cassette Verifying and recalibrating the dispensing cassette Accuracy verification procedure 82 Thermo Scientific Multidrop Combi User Manual Disposable gloves High purity water deionized distilled water Photocopy of the accuracy verification worksheet fro

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