Mercury Testing for Water Testing Detection Range
Contents
1. Do Not press Start until the sample is ready Note Unit may go into screen saver mode after a few minutes Press any button to 4 Pouch Open the sensor pack which contains the resume Do not press and hold ON OFF sensor colored plastic housing and plastic cuvette Important The pack contains a transparent desiccant pouch This should be blue in color If desiccant has turned completely pink in color sensor may not perform well PA 5 Cuvette Place the plastic cuvette in the instrument 6 Sensor Place colored plastic sensor on the cuvette Any orientation Make sure sensor is seated securely on the cuvette ANDalyze Inc 2109 South Oak St Suite 102 Champaign IL 61820 USA Tel 1 217 328 0045 www andalyze com Page 6 of 11 Mercury Testing for Water Testing April 2014 7 Sample Tube Collect water to be tested in a clean cup not provided Add approximately 4 drops hydrogen peroxide per 50ml water except for tap water sample mix well 8 Slowly pour the test water into the provided sample tube containing liquid buffer up to the 5 mL mark If required use one of the provided disposable plastic pipettes to transfer water accurately without exceeding 5 mL volume mark Close cap tightly and mix well by shaking Important Open tube carefully so that liquid buffer does not fall out Do not exceed the 5 mL mark for accurate results 9 Syringe With one of the provided syringes draw 12. best results use freshly collected sample unpreserved in acid for analysis We recommend that to every 50 mL of sample water add approximately 4 drops of hydrogen peroxide from provided dropper bottle except for tap water sample and mix This is to improve mercury testing accuracy We recommend that you use the sample within 1 hour maximum of 2 hours of collection to minimize any metal loss to the walls of the sample container Once the sample is mixed with ANDalyze sample buffer test within 15 minutes Temperature Range ANDalyze test kits work in the 17 30 C 63 86 F temperature range However the most accurate and precise results are obtained in the range of 20 25 C 68 77 F pH Range The ANDalyze sample buffer that is provided in the sample tubes brings the pH of the test solution to pH 7 4 Generally the raw sample water can be in the range of pH 5 pH 8 If you have a sample which is acidic or basic please check the pH first to confirm it is within the range prior to mixing with the ANDalyze buffer The final buffered test solution should be pH 7 4 for best results Note Our tests have shown that environmental samples preserved in acid to a pH lt 2 cannot usually be brought to a pH of 7 4 when mixed with the ANDalyze buffer These samples have to be first neutralized with NaOH to a pH 7 before mixing with ANDalyze buffer Contact ANDalyze customer service for instructions related to pre treatment of high
3. uL of the provided mercury standard solution Close the cap and shake well Metal standard spiked for on site calibration is required to incubate for all environmental water samples Add this spiked solution to a new sample tube up to the 5 mL mark Shake well and analyze 1 mL of the sample using a new mercury sensor and cuvette If the result of this spiked sample is negative the sample matrix leads to false negatives due to interference contact ANDalyze customer service or test for mercury in a lab ANDalyze Inc 2109 South Oak St Suite 102 Champaign IL 61820 USA Tel 1 217 328 0045 www andalyze com Page 8 of 11 Mercury Testing for Water Testing April 2014 4 Technical Specifications for ANDalyze Sensors 4 1 Detection in Drinking Water ANDalyze s proprietary Catalytic DNA sensor uses a DNAzyme reaction that fluoresces in the presence of the target contaminant lead uranium copper mercury etc The fluorescence of the reaction is measured using the AND 1000 fluorimeter to determine the concentration presence of the free analyte ion Pb UO Cu Hg etc in solution Associated US Patents 8 815 156B2 6 706 474 7 192 708 6 890 719 7 332 283 Materials Used ANDalyze Fluorimeter a Mercury Sensor Kit Analyte Metal Standard Solution chap Performance Mercury dilutions containing 0 150 ppb Hg were prepared in DI water Five replicates were used for each test at each dilution Mercury Detect
4. AN Dalyze Mercury Testing for Water Testing Detection Range Positive Negative at 2ppb Up to 50ppb April 2014 Edition 3 ANDalyze Inc 2012 All rights reserved Printed in USA Mercury Testing for Water Testing April 2014 Table of Contents 2 EE eN IO aTe LA TA AEA vecian Savas E deena nanan olen oa as aoa borat cad ooesnc ds 3 2 1 Qualitative Mercury Sensor 2 2 Water Testing Guidelines 2 3 Sensor Pack Cuvette and Sensor 2 4 Inserting Cuvette and Sensor 2 5 Sample Injection and Measurement 2 6 Pipetting Use Guidelines Be OSI E cs SALTO Li opehs sins Sessa cusesea aru ndinsisaiua teats wees incauudavas Gioia A E duindla EE EE TE 6 3 1 Required Materials 3 2 Testing a Sample 3 3 Results 3 4 Waste Disposal 3 5 Test for False Positive 3 6 Test for False Negative 4 Technical Specifications for ANDalyZe SCNSOSPS cccccsssssssssecsecessesseeseecoeceesesseseeecaesessesusseescaesesseasees 9 4 1 Detection in Drinking Water 4 2 Interference 4 3 Product Accuracy Ranges 5 Consumables and Replacement It MS ccccccssesscsssecsecessesseseesssecescesseaescaeseeseecesseseeecaeseesesaeseaeeaeses 10 6 Customer Service Contact INFOrMAtiON cccscssecsscescessesseecsececessesesceecsecessesussaescaesesseaseeseascaeseeaes 11 ANDalyze Inc 2109 South Oak St Suite 102 Champaign IL 61820 USA Tel 1 217 328 0045 www andalyze com Page 2 of 11 Mercury Testing for Water Testing April 2014 2 General Informatio
5. Number ANDO14 o Equipment for 25 Tests Kit Includes 25 Sensor Packs with Sensor amp Cuvette 25 Sample Tubes with buffer 25 1 mL Syringes 15 Disposable Transfer Pipettes 1 dropper bottle of Hydrogen Peroxide 3 mL Mercury Standard Solution 1 ppm Hg Tube containing Mercury Chelator pellets 2 50 mL tubes for testing false negatives Instruction Manual Material Safety Data Sheets MSDS ANDalyze Inc 2109 South Oak St Suite 102 Champaign IL 61820 USA Tel 1 217 328 0045 www andalyze com Page 10 of 11 Mercury Testing for Water Testing April 2014 6 Customer Service Contact Information Contact us by Email info andalyze com sales andalyze com By Telephone 866 364 7173 Toll Free in US or 1 217 328 0045 By Fax 1 857 386 1277 Business Service Hours 8 00am to 5 00pm Central Standard Time USA Monday through Friday Company Address ANDalyze Inc 2109 South Oak St Suite 102 Champaign IL 61820 Website www andalyze com ANDalyze Inc 2109 S Oak Street Suite 102 Champaign IL 61820 USA Email info andalyze com Telephone 1 217 328 0045 ANDalyze Inc 2109 South Oak St Suite 102 Champaign IL 61820 USA Tel 1 217 328 0045 www andalyze com Page 11 of 11
6. ion Range 2 50 ppb mercury Above 50 ppb mercury there can be false negatives If it is known that sample may contain very high levels of mercury please dilute your sample in DI water Note All specifications are subject to change without notice 4 2 Interference Interfering ion Interference Above tolerance level level will cause Interference tests were done witha Oor 1 Calcium Ca 80 ppm False negative ppb mercury in DI water plus the potential interfering ion The interference tolerance levels represent the Iron Fe 15 ppb False negative concentration above which will there is a false positive for a 0 ppb mercury sample or false negative where mercury is present Data represents an average of at least three replicates Dihydrogen phosphate H PO 2000 ppm False negative Bicarbonate HCO 800 ppm False negative Zinc Zn 200 ppm False positive Cobalt Co 140 ppm False positive Cadmium Cd 60 ppm False positive Nitrate NO3 gt 10000 ppm No interference Sodium Na gt 3700 ppm No interference ANDalyze Inc 2109 South Oak St Suite 102 Champaign IL 61820 USA Tel 1 217 328 0045 www andalyze com Page 9 of 11 Mercury Testing for Water Testing April 2014 5 Consumables and Replacement Items Fluorimeter Part Number ANDOO2 o Capable of measuring multiple metals o Includes Fluorimeter USB to MINI B Cable 100uL Fixed Volume Pipette and Tips pH Test Strips Sensor Kit Part
7. ly acidic or highly basic samples ANDalyze Inc 2109 South Oak St Suite 102 Champaign IL 61820 USA Tel 1 217 328 0045 www andalyze com Page 3 of 11 Mercury Testing for Water Testing April 2014 2 3 Sensor Pack Cuvette and Sensor Sensor Pack Each sensor pack contains a cuvette a sensor and a desiccant These are single use and must be discarded The desiccant should be blue in color If it has turned completely pink in color the H ed sensor may not perform well 2 2 4 Inserting Cuvette and Sensor Cuvette The cuvette has an arrow which should face you when inserted Insert the cuvette completely so that the fluorimeter lid can close The meter and cuvette design helps to prevent improper orientation Sensor The square portion of the sensor can be placed on the cuvette in any orientation with the round sections facing upward Sensors can only be used once and should be disposed of immediately after use 2 5 Sample Injection and Measurement Fluorimeter instrument should be laid flat on a stable surface during a measurement A buffered solution is prepared in a sample tube as described in the test section 4 which is used for measurements A new syringe should be used to withdraw 1 mL water from a sample tube This syringe can be attached to the top of the housing as shown in the picture The sample should be injected through the housing into the cuvette at a constant speed of 3 5 seconds The syringe and housing sh
8. mL of buffered sample water from the sample tube into syringe 10 Attach the syringe to the housing over the cuvette in the instrument Syringe tip will fit into top of sensor Note If the unit is left on for more than a few minutes without any activity a screen saver black screen will be activated press any button to resume operation Do not press and hold ON OFF The instrument automatically turns off if not used for more than 5 15 minutes user settable 11 Maintaining a constant speed over 3 5 seconds carefully squeeze the buffered sample water through the housing into the cuvette 12 Quickly remove sensor housing and syringe and close sample chamber door 13 Immediately press the START button located just below the screen 14 Remove cuvette when complete 3 3 Results The sample will be tested and results displayed in a negative or positive manner A result of POSITIVE indicates that the level of Mercury in the sample is Sensor Mereury ABOVE 2ppb while a result of NEGATIVE indicates the level is BELOW pos T UE 2ppb ID Hy 744 To save the results press the SAVE button Save Ree ANDalyze Inc 2109 South Oak St Suite 102 Champaign IL 61820 USA Tel 1 217 328 0045 www andalyze com Page 7 of 11 Mercury Testing for Water Testing April 2014 3 4 Waste Disposal All waste produced during the test can be disposed of in normal public receptacles Materials and liquids used are not con
9. n Important Before continuing please read the entire ANDalyze Fluorimeter User Manual Pay attention to all danger warning and caution statements Failure to do so could result in serious injury to the operator or damage to the equipment Make sure that the safety features of this equipment are not impaired do not use or install this equipment in a manner other than that specified in this manual If the equipment is used in a manner not specified by the manufacturer the safety features of this equipment may be impaired and injury to the operator or damage to the equipment may result 2 1 Qualitative Mercury Sensor The mercury sensor is a qualitative sensor that shows a Positive Negative result A Positive test result indicates that mercury is present at levels greater than 2 ppb A Negative result indicates the water contains less than 2ppb mercury This test does not require on site calibration Important Certain levels of interfering ions may result in a false positive or false negative Because of this instructions to test for these are included below 2 2 Water Testing Guidelines Note The below steps are for testing for dissolved bio available metal ions in drinking water samples These steps can also be used for other matrices such as surface ground industrial and wastewater which have been pre treated Contact ANDalyze customer service for additional application notes on pre treatment methods Water Sampling For
10. ould be immediately removed and the sample door closed The START button located just below the screen should be pressed immediately to start any measurement Important After each analysis discard all components used during the analysis including cuvette sensor housing sample tube and syringes to avoid cross contamination ANDalyze Inc 2109 South Oak St Suite 102 Champaign IL 61820 USA Tel 1 217 328 0045 www andalyze com Page 4 of 11 Mercury Testing for Water Testing April 2014 2 6 Pipette Use Guidelines 1 New Pipette Tip Attach a new tip by placing the end of the pipette into one of the available tips and pressing down on the pipette body Note Tips are disposable and should never be used more than once Use of tips helps prevent contaminating the pipette 2 Depress the plunger button on the top to the first stop see photo at left DO NOT depress all the way to the pipette body 3 Immerse the clean tip into the solution to be withdrawn 4 Release the pressure slowly to withdraw the solution into the tip Important Make sure that the pipette tip continues to be immersed in the solution during release so as to not expose the tip point to air 5 Remove the pipette from the solution Note The liquid level in the tip should be approximately at the 2 graduation 6 Immerse the tip into the liquid present in the sample tube where the withdrawn solution is to be dispensed 7 Slowly depress
11. sidered harmful to people or the environment and do not require special disposal The plastic waste is made from recyclable plastics and can be disposed of per local guidelines 3 5 Test for False Positive If your test result for mercury is positive the validity can be tested by testing against a false positive by adding a mercury chelator and analyzing a chelated sample In anew sample tube add the test water up to the 5 mL mark as described before To this add one pellet of the Mercury Chelator Close the cap and shake well The pellet will NOT dissolve Analyze 1 mL of the Chelator containing sample using a new mercury sensor If the result of this sample containing Mercury Chelator is positive this sample matrix may have led to a false positive Contact ANDalyze customer service 3 6 Test for False Negative If your test result for mercury is negative the validity can be tested by testing against a false negative Required Material ANDalyze Fluorimeter 1 Sensor Pack with Sensor amp Cuvette 1 1mL Syringe F 1 Buffer Solution Tube 9 1 50 mL Self Standing Tube To be Re used E Mercury Standard solution 1 ppm 1 Hydrogen peroxide dropper bottle 1 Transfer Pipette 1 Sample Cup Not Provided Prepare a spiked sample by adding 50 mL of sample water to one of the provided 50 mL tubes Add 4 drops of hydrogen peroxide from provided dropper bottle except for tap water sample To this add 100
12. the operating button ALL THE WAY to dispense the liquid contained in the pipette tip See photo at right 8 Remove the pipette and discard the used tip Note Dispose of tips immediately after use to prevent possible contamination of the pipette ANDalyze Inc 2109 South Oak St Suite 102 Champaign IL 61820 USA Tel 1 217 328 0045 www andalyze com Page 5 of 11 Mercury Testing for Water Testing April 2014 3 Testing a Sample This section will walk you through the process of testing a water sample for the desired analyte Please read this entire section before beginning the test as parts of the test will be time sensitive Be aware and pay attention to all notes 3 1 Required Materials ANDalyze Fluorimeter 1 Sensor Pack with Sensor amp Cuvette 1 1 mL Syringe 1 Sample tube with buffer 1 Transfer Pipette A 1 Clean sample collection cup Not Provided 1 Hydrogen peroxide dropper bottle Note Before using the ANDalyze Fluorimeter assure that it is charged See Section 4 1 in the ANDalyze Fluorimeter User Manual for Battery Charging Information 3 2 Testing a Sample 1 Start Up Initialize the instrument by pressing the ON OFF button Instrument will initialize in about 1 2 A e seconds Sensor Mercury Site DEH Time 99 24 12 30 Press SELECT to Edit 2 Sensor Type Ensure that the fluorimeter is on the correct metal screen 3 Site Confirm the site name
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