Microman User`s Guide
Contents
1. MIiCROMAN Other languages can be consulted and downloaded on our website www gilson com MICROMAN CONTENTS page 1 INTRODUCTION 2 2 PARTS CHECK 3 3 DESCRIPTION 4 4 OPERATING RANGES AND MATERIALS 5 5 USING MICROMAN 5 6 FITTING THE CAPILLARY AND PISTON 6 7 SETTING THE VOLUME 8 3 PIPETING 0000 gt 9 GLP FEATURES 11 10 TROUBLESHOOTING 11 11 CLEANING AND DECONTAMINATION 12 12 SPECIFICATIONS 14 1 INTRODUCTION Congratulations on acquiring your new Microman pipette Microman is suitable for many different types of application including pipetting volatile viscous dense or high surface tension liquids It is a quality product from Gilson which is fully ISO8655 compliant CE labeled conforms to the EC directive on in vitro diagnostic medical devices The six models permit precise and accurate pipetting of viscous dense and vaporous liquids They also allow contamination free pipetting avoiding vapor contamination and cross contamination M25 M50 and M250 with thin and long CPs are especially suited for use with long or narrow vessels M10 and M100 autoclavable models are especially suited for 100 contamination free pipetting Enjoy the following features 2 Microman pipettes are equipped with a posi tive displacement mechanism thatisolates the aspirated liquid from the body of the pipette The positive displacement mechanism pre vents the sample to sample con2. 6 Check that the parts are dry before reas sembling the pipette 7 Set the pipette aside to stabilize at room temperature 14 12 SPECIFICATIONS Microman is a high quality pipette that offers excellent accuracy and precision The figures given inthe Gilson Maximum Permissible Errors table were obtained using Gilson CPs These figures are only guaranteed by using genuine Gilson CPs Each pipette is inspected and validated by qualified technicians according to the Gilson Quality System Gilson declares that its manufactured pipettes comply with the requirements of the ISO 8655 Standard by type testing The adjustment is carried out under strictly defined and monitored conditions ISO 8655 6 e Basis of adjustment Ex Reference temperature 20 C Relative humidity 50 Barometric pressure 101 kPa Use of distilled water grade 3 ISO 3696 e Ten measurements for each test volume which are Nominal Volume 50 of Nominal Volume and the minimum or 10 of Nominal Volume lt gt The data given in the table conform to the ISO 8655 2 Standard MICROMAN Specifications Table Maximum Permissible Errors Model Volume Gilson ISO 8655 Reference uL Systematic Random Systematic Random error uL error uL error uL error uL Min 1 0 09 lt 0 03 0 20 0 M10 5 0 10 3 0 20 lt 0 10 F148501 Max 10 015 06 0 20 lt 0 10 Min 3 0 25 9 006 0 70 09 M25 10 0 27 lt
3. labeled 10 TROUBLESHOOTING You may be able to identify and to correct the problem by reference to the following table If you can t solve the problem contact your Gilson representative Before returning any pipette ensure that A itis completely free of chemical biologi cal or radioactive contamination Use the safety bag provided by Gilson Leaks change the capillary and piston No stroke the piston is not properly fitted into the clamp check that the piston is seated cor rectly refer to pages 6 7 Difficult to fit a capillary clean the capillary holder with ethanol Inaccuracy you should check the capillary is correctly mounted on the capillary holder refer to pages 6 7 Imprecision change the capillary and piston 11 MICROMAN Difficult set the volume this suggests that the pipette is damaged internally in which case you should contact your Gilson representative If the problem persists Contact your Gilson representative 11 CLEANING AND DECONTAMINATION Microman is designed so that the parts normally in contact with liquid contaminants can easily be cleaned and decontaminated Cleaning The pipette must be cleaned with soap solution before it is decontaminated External 1 Wipe the entire pipette with a soft cloth or lint free tissue impregnated with soap solu tion to remove all dirty marks If the pipette is very dirty a brush with soft plasti
4. 0 08 0 70 00 F148502 Max 25 030 0 1070 9 0 30 M50 Min 20 0 34 lt 020 070 lt 0 30 F148503 Max 50 0 70 lt 0 30 070 lt 0 30 Min 10 050 lt 0 20 1 50 10160 M100 50 420785 0 30 zlo S 0100 F148504 Max 100 1 00 lt 0 0 0 Min 50 1 50 lt 030 600 lt 2 00 M250 100 170 lt 030 600 lt 2 00 F148505 Max 250 250 lt 050 600 lt 2 00 Min 100 3 00 lt 1 60 12 00 lt 4 00 M1000 500 500 lt 2 50 12 00 lt 4 00 F148506 Max 1000 800 lt 4 00 12 00 lt 4 00 Performance Tests Each pipette is inspected and validated according to the Gilson Quality Assurance System Based on extensive historical data manufacturing con ditions and expertise and in compliance with ISO standards relative to statistical process control the assurance level of this instrument performing to specifications is 99 8 Specifications rely on the quality and consistency for the whole pipet ting system they are guaranteed only when the pipette is used with Gilson capillary pistons 16 DOQOOODO0O0 DOOD OOO OO 99 EC DECLARATION OF CONFORMITY Q The company GILSON S A S 19 avenue des Entrepreneurs BP 145 95400 Villiers le Bel France Tel 33 0 1 34 29 50 00 Fax 33 0 1 34 29 50 20 www gilson com Hereby certifies on its sole responsibility that the products listed below Microman M10 M25 M50 M100 M250 M1000 Positive displacement Capillaries Pistons comply with the requiremen
5. c bristles may be used 2 Wipe the entire pipette with a soft cloth or lint free tissue impregnated with distilled water 3 Leave the parts to dry by evaporation or wipe them with a clean soft cloth or lint free tissue Internal The following components only can be immersed in a decontaminant solution clamp assembly return spring and capillary holder shaft 1 Unscrew the capillary holder shaft 2 Remove the clamp assembly and return spring from the shaft 12 3 Set aside the body handle in a dry and secure location 4 Clean the individual components using an ultrasonic bath for 20 minutes at 50 C or with a soft cloth and brushes Small round brushes with soft plastic bristles may be used to clean the interior of the shaft 5 Rinse the individual components with distilled water 6 Leave the parts to dry by evaporation or wipe them with a clean soft cloth or lint free tissue 7 Reassemble the pipette Decontamination You may refer to the Decontamination Procedure LT802288 available at www gilson com Chemical Decontamination The pipette should be cleaned before it is decontaminated Full details of recommended decontamination procedures for Gilson pipettes are available from your supplier Whatever other decontaminant you use check with the supplier of the decontaminant that it is compatible with the materials used in the construction of the pipette and does not attack either of the fol
6. e not to damage the sealing tip when you handle the piston 3 1 Verify that the model of capillary piston identified on the Tipack security sticker on the rack corresponds to the Microman you are preparing for use CP25 is for use with the M25 CP50 is for use with the M50 and CP250 is for use with the M250 Press the push button to the second stop to open the clamp NO 00 Select a piston and slide the stem 2 fully into the clamp 4 Slide the mounted piston into the capillary 4 5 Gently push the capillary until it snaps onto the capillary holder 6 Microman M10 M100 and M1000 The capillary pistons CP are delivered already assembled To fit a capillary piston follow this procedure 1 Verify that the model of CP capillary piston identified on the Tipack security sticker on the rack corresponds to the Microman you are preparing for use CP10 is for use with the M10 CP100 is for use with the M100 and CP1000 is for use with the M1000 2 Press the push button on the Microman past the first stop position to the second stop position The piston clamp should now be open and protruding from the capillary holder 3 Slide the Microman piston clamp inside the top of the capillary tube until the piston clamp is halfway down the piston mounting stem 4 Slowly release the push button while pushing the body of the Microman into the capillary piston until it is firmly seat
7. ed on the Microman MICROMAN 5 Continue releasing the push button to ensure that the piston is secured by the piston clamp 6 To ensure thatthe piston is correctly seated and therefore calibrated slowly press the push button until you feel and hear a slight click 7 SETTING THE VOLUME The volume of liquid to be aspirated is set using the volumeter The volumeter consists of three number dials which are read from top most significant digit to bottom least significant digit A marker is used to set exact or intermediate volumes using the scale on the bottom dial The dials are colored either black or red to indicate the position of the decimal point according to the model see examples M10 M100 M25 M50 M250 M1000 oy 0 ed tel Wel 6 I8 7 a A A 2 6 8 uL 68 uL 6 8 uL 06 8 uL 168 uL 0 75 mL Model Color of volumeter numbers Black Red M10 M25 M50 uL 0 1 uL M100 M250 uL none M1000 0 1 and 0 01 mL mL The volume is set by turning the thumbwheel slowly to reach the required setting To obtain maximum accuracy when setting the volume pro ceed as follows when decreasing the volume setting slowly reach the re quired setting making sure not to overshoot the mark when increasing the volume setting pass the required value by 1 3 of a turn and then slowly decrease the volume to reach the required setting making sure not to overshoot the mark To avoid parallax
8. errors make sure that the vol ume indicator and the selected volume marking are in your direct line of vision At close range you may find it helps to close one eye MICROMAN 8 PIPETTING Aspirating press the push button to the first stop 1 immerse the capillary 2 mm into the liquid r slowly release the push button to draw up the liquid top gt Z position wipe any liquid from the outside of the cap illary taking care notto touch the orifice Dispensing place the end ofthe capillary againstthe inside wall of the recipient vessel press the push button slowly to the first stop keeping the push button depressed move the capillary away from the side wall withdraw Microman from the vessel and re lease the push button Ejecting the Piston capillary Press the push button to the first stop 1 then press harder to the second stop 2 at this point the piston and capillary are ejected simultaneously 9 GLP FEATURES These are as follows No need to touch disposables capillary piston Serial Number engraved on body of the pipette 10 Bar Code on the box and with the certificate can be trans ferred ID Tag Application or User Colour coded push button blue for standard Microman red for autoclavable Microman Colour coded magnifier with volume range Gilson certificate of conformity according to ISO8655 Microman and CP are CE IVD
9. etting the volume Volumeter 4 OPERATING RANGES AND MATERIAL Available Models Table 1 Model Volume Range Autoclavable M10 1 pL to 10 uL Yes M25 3 UL to 25 pL No ONG M50 20 uL to 50 pL No M100 10 uL to 100 uL Yes M10 M250 50 uL to 250 No M1000 100 uL to 1000 uL No Materials Table 2 ModelSpring Clamp Shaft Body Capillary Piston C P CP Holder B C P M10 SS BeAlloy Polyester PVDF Polypropylene Polyester 0 M25 SS BeAlloy Polyester PVDF Polypropylene Polyester 5 M50 SS BeAlloy Polyester PVDF Polypropylene Polyester 0 M100 SS BeAlloy Polyester PVDF Polypropylene Polyethylene CP100 M250 SS BeAlloy Polyester PVDF Polypropylene Polyethylene CP250 M1000 55 BeAlloy Polyester PVDF Polypropylene Polyacetal CP1000 RS SS Stainless Steel Be Beryllium PVDF Polyvinylidene Fluoride 5 USING MICROMAN Three simple steps are necessary 1 Mount the capillary piston 2 Set the volume 3 Aspirate and Dispense 3 Microman is automatically calibrated by cor rectly fitting a capillary piston After setting the volume the volume measured will be accurate and precise MICROMAN 6 FITTING THE CAPILLARY AND PISTON Never lubricate the capillary holder or any other part of the pi pette If the capillary tends to slip off the capillary holder clean the capillary holder with ethanol using a medical wipe or similar soft tissue Microman M25 M50 and M250 Take car
10. low ing plastics Polyester or PVDF Polyvinylidene Fluoride Non immersible Parts 1 Wipe the body handle of the pipette with a soft cloth or lint free tissue impregnated with the chosen decontaminant 2 Wipe the body handle of the pipette with a soft cloth or lint free tissue impregnated with distilled water or sterilized water MICROMAN Immersible Parts The following components only can be immersed in a decontaminant solution clamp assembly return spring and capillary holder shaft 1 Unscrew the capillary holder shaft 2 Remove the clamp assembly and return spring from the shaft 3 Set aside the body handle in a dry and secure location 4 Immerse the components in the decontami nant solution or wipe them according the instructions given by the manufacturer or supplier of the decontaminant 5 Rinse the individual components with distilled or sterilized water 6 Leave the parts to dry by evaporation or wipe them with a clean lint free tissue or a soft cloth 7 Reassemble the pipette Autoclaving Only Microman M10 and M100 are autoclavable The four other models Microman M25 M50 M250 and M1000 are not autoclavable 1 Unscrew the capillary holder shaft 2 Remove the clamp assembly and return spring from the shaft 3 Clean the parts to be autoclaved especially the shaft 4 Put the parts in an autoclaving sack 5 Autoclave for 20 minutes at 121 C 0 1 MPa
11. tamination that can result from the aerosol effect Microman uses disposable capillaries and pis tons Changing these parts which must be the only ones to make contact with the aspirated liquids can provide absolute protection against the contamination caused by carry over from one sample or reagent to another You simply have to change the capillary and piston be tween each assay they are automatically and simultaneously ejected thus avoiding any risk to the operator Microman is equipped with a direct reading volumeter that allows precise and continuous adjustment of the required volume Microman is permanently calibrated at the manufacturing stage the capillary and piston are positioned automatically therefore it requires no further calibration Microman requires no lubrication or mainte nance because of the nature and quality of the materials used in its construction 2 PARTS CHECK LIST Just take a moment to verify that the following items are present Microman User s Guide Safety bag Adhesive id tags strip of 6 Capillary pistons 10 Certificate of conformity including bar code sticker MICROMAN 3 DESCRIPTION 1 Color coded push button red for autoclav able Microman and magnifier with volume range for aspirating and dispensing 2 Body or handle Capillary 9 cp 4 Piston 5 Capillary piston holder 6 Shaft 7 Thumbwheel for s
12. ts of the following European 98 791 EC Directive on In Vitro Medical Diagnostic Annex Ill self declared Villiers le Bel September 14th 2005 Atika El Sayed Herv Ledorze General Manager Quality Manager 4 GILSONGS 0 00 000000 D0 009 DOO DOOD OO JOOOD OJOS JONSON JO DOOMONJO OCOD NOOO gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt gt POOOOOOOOOOOOOOOOOOOOMOOOOOOOOJOOOJOOOOOOOOMOOOJOOJOOOOOMOOOOJOOOOOOMOOJOOJOOOOOOOMOJOOJOOOOOMOJOOOOOOOMOOJOOOJOOOOOOOOOOOOOJOOOOOOOMMOOOOJOOJOOOOOMOOJOOOOOOOOOMOOOJOOJOOOOOMOOOOOOO 17 MICROMAN NOTES 19 LT801502 B 62006 Gilson SAS All right reserved September 2006 English Printed in France World Headquarters Gilson Inc 3000 Parmenter Street P O Box 620027 Middleton WI 53562 0027 USA Telephone 1 800 445 7661 or 1 608 836 1551 Fax 1 608 831 4451 Gilson SAS 19 avenue des Entrepreneurs B P 145 95400 Villiers le Bel France Telephone 33 1 34 29 50 00 Fax 33 1 34 29 50 20
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