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1. a step ahead gt O OO cl o z I O WI Ke OO D So SE 3 0 cq e Oo gt 5 O O O ep O O be See H S glia il er EE 04 Micro Flask by Duetz CG RAMbio 08 Anaerobic Fermentation Monitor 10 Mini bioreactors 12 Glass autoclavable bioreactors een 16 Photo bioreactors 18 Single use bioreactors 22 Bi oBundle cultivation systems E 26 my Control 8 28 in Control i 30 ez Control 32 i Control 34 Bioxpert _ 38 DO Sensors 40 pH Sensors 7 42 BioSenz glucose lactate analyzer 44 BioSep acoustic cell retention system 46 Steam I n Place bioreactors 48 Special projects 50 AppliCare Content Applikon Biotechnology Cultivation Systems from Discovery to Production 08 Applikon Biotechnology a step anead Applikon Biotechnology is a world leader in developing and supplying advanced bioreactor systems from laboratory scale to production scale We partner Seen with our customers to implement scalable platforms from initial screening through development and full scale production This minimizes scale up risks and guarantees the shortest time to market for our customers new product development New technologies Applikon is Known for bringing new technologies to the market These new technologies offer advantages in process efficiency for research and development as well
2. panels can be retrofitted to the Applikon bioreactors to turn your standard bioreactor into a photobioreactor with no downtime and minimal investment oo Features e Proven bioreactor design e Photo synthetically Active Radiation PAR e Long lifetime 100 000 hrs in combination with 400 700 nm ideal for plant growth to achieve good lumen maintenance better efficiency in the response of plants e Low voltage operation having more than 90 al e Excellent wavelength stability over time efficiency with no EMI radiation consistent high reliability e Directional light output A even distribution e Usable wavelength blue 470 nm and red 660 nm of light over the target application area for plant growth e Great performance in cold environments e Fully regulated form O to 100 light intensities e No heat generation O to 20 000 lux in real time Applications e The PhotoBio can be used for all photosynthesizing organisms plants ER algae bacteria Specifications Maximum driving capacity Programmable zones Intensity control Time base Time range Light driving Housing amp Protection Class User interface Serial Communication port Ambient temperature Power supply Power consumption 140 watt of power or 24 light bank of 6 watt 8 0 to 100 PWM Built in Real time clock 99 59 hours minutes per step maximum 9 time intensity steps in one cycle Constant current sour
3. Spare I O 8 x digital output 4 x analog out 0 4 20 mA Additional features Emergency stop connection central alarm output System status indication inactive active alarm by integrated colored light Power Pmax 480 VA 115 230 Vac Dimensions d x w x h 350 x 190 x 400 mm D x W x H Weight kg 8 5 kg 2 8 Applikon Biotechnology Cultivation Systems from Discovery to Production in Control simply powerful in Control is a process controller for laboratory scale bioreactors The system is a drop in replacement for our older ADI 1010 Bio Controller and ADI 1030 Bio Controller The in Control offers high level control on a small footprint When you are running your bioreactors controlled by our ADI 1010 or ADI 1030 Bio Controllers the in Control can be used to increase the capabilities of your bioreactor system with a minimal investment and no downtime for your process The controller can be used for both cell culture and microbial cultures The intuitive human interface is a built in color touch screen Features e Control of all bioprocess parameters including pH temperature dissolved oxygen agitation foam and level e Selectable autotuning adaptive PID control for accurate control when process conditions change during the culture e Easy operation through touch screen interface and through web browsers a Windows PC Apple PC Linux PC iPhone iPad Android table
4. 2000 rpm 1 rom Analog in 8x Analog out 4x Digital in 8x Digital out 8x Fluorophor pH Optional 5 5 8 5 pH 0 1 pH Fluorophor Dissolved Oxygen Optional O 100 saturation 0 1 Biomass Optional Display 10 4 Inch color display with resistive touchscreen Communication interfaces 1 x Ethernet port computer communication 2 x USB slave 1 x USB master Control type PID control with selectable auto tuning adaptive control Actuators Up to 3 fixed speed pumps Up to 4 Mass Flow Controllers Heating and cooling via thermocirculator heating blanket cooling water valve Water cooled condenser connection Additional features Emergency stop connection central alarm output Color touch screen operation Process connections 2 x Gas outlet water for condenser in and out Water for temperature control in and out Power 230 Vac 50 Hb 115 Vac 60 Hb Dimensions dxwxh 474 x 408 x 711 mm Weight kg 30 kg 32 Applikon Biotechnology Cultivation Systems from Discovery to Production Control the scalable control system i Control is an easy to operate bioreactor control system and utility console SE This scalable control solution is used to control processes in bioreactor systems from laboratory scale to pilot plant and production scale saving time and money during scale up The i Control is available in a Single a Dual bioreacto
5. 5 22 1 9 2 liter jacketed 2 2 1 7 0 5 2 2 1 9 3 liter single wall 3 1 Set 0 5 1 9 1 5 3 liter jacketed 3 1 2a 0 5 1 9 1 5 5 liter single wall 4 8 3 4 0 9 1 6 1 1 5 liter jacketed 4 8 3 4 0 9 1 6 ql 7 liter single wall 6 8 5 4 1 5 2 2 1 8 7 liter jacketed 6 8 5 4 1 5 2 2 1 8 15 liter single wall 16 5 12 3 0 ALF 1 5 15 liter jacketed 18 2 12 3 0 L 1 2 20 liter single wall 23 4 16 3 0 2 4 2 0 Internal Diameter mm Internal Height mm Autoclave dimensions WxH mm 1 liter 95 200 9172 x 393 2 liter single wall 105 240 190 x 436 2 liter jacketed 105 240 9219 x 486 3 liter single wall 130 240 0190 x 436 3 liter jacketed 130 240 0235 x 436 5 liter single wall 160 250 2260 x 436 5 liter jacketed 160 250 2260 x 480 7 liter single wall 160 350 2260 x 600 7 liter jacketed 160 350 0264 x 645 15 liter single wall 222 440 0381 x 710 15 liter jacketed 240 440 0391 x 740 20 liter single wall 222 620 2381 x 900 Drive system Direct drive lipsealed or magnetically coupled Maximum stirrer speed rpm Standard range is 50 1250 1 2 and 3 liter systems can be supplied with 2000 rpm motor Impellers Rushton and marine with outside diameters 45 mm 60 mm 75 mm or 85 mm Gas sparger Porous sparger or L type sparger Gas overlay Yes Exhaust gas Water cooled exhaust gas condenser Sampling Fixed height or height adjustable sample pipe with optional sampling system Sa
6. Biotechnology Cultivation Systems from Discovery to Production 09 Total volume liter 6 reactors of 250 ml or 500 ml Working volume liter 200 ml or 400 ml Minimum working volume liter 50 ml or 100 ml Drive system Magnetically coupled drive Maximum stirrerspeed rpm Standard range is 50 500 Impellers Marine type Exhaust gas Exhaust gas connection Temperature Measurement Pt 100 sensor in central stirrer bar Control heating via central stirrer bar Dimensions DxWxH 30 x 85 x 67 cm Empty Weight kg 75 kg 1 O Applikon Biotechnology Cultivation Systems from Discovery to Production Mini bioreactors Tool small real bioreactors The MiniBio range of bioreactors 250 ml 500 ml and 1000 ml total volume is a true scale down of the laboratory scale bioreactor The MiniBio systems have the same flexibility as the laboratory scale bioreactors This means that the MiniBio systems can be customized to fit the demands of any process The small volume reduces media costs and maximises bench space which is normally at a premium Features Applications e Generate more data in less time e Screening studies e Easy setup and operation e Media optimization e Cultivate using less medium e Process optimization e Cultivate using less bench space e Microbial and Cell culture e Generate scalable results Batch Fed Batch Perfusion
7. Electrode slope S4 7 gt 98 _ Isothermal Intersection pH Eiso 6 5 8 gg PH Range 4 9 Membrane resistance 25 C ohm lt 1200 Membrane resistance 37 C ohm lt 500 Calibration drift in Buffer 9 mV min lt 2 _ Stirring Error Buffer 9 25 C pH lt 0 05 RS Drift 72 hours after Autoclaving pH lt 0 13 Zero point drift per week in PBS pH lt 0 05 Response Time pH 9 to 4 25 C in Seconds lt 90 Response Time 90 pH 9 to 4 25 C in Seconds lt 45 lt Response Time pH 9 to 4 37 C in Seconds lt 45 Response Time 90 pH 9 to 4 37 C in Seconds lt 45 Insertion length o 12 mm sensors Z001012051 insertion length 120 mm 170 mm total length used with Tracfix sensor holder 2001023551 insertion length 235 mm 285 mm total length Bioreactor 1 5 L 2001032551 insertion length 325 mm 375 mm total length Bioreactor 7 L 2001042551 insertion length 425 mm 475 mm total length Bioreactor 15 L 2001059051 insertion length 590 mm 640 mm total length Bioreactor 20 L Insertion length o 8 mm sensors Z001015080 insertion length 150 mm gt Z001017080 insertion length 170 mm pH Sensor in TracFix Sensor electrical connection K9 retractable sensor holder 4 P Applikon Biotechnology Cultivation Systems from Discovery to Production Blosenz glucose lactate analyzer The Applikon BioSenz is a unique measur
8. and Continuous cultivation Specifications Color your lab Applikon Biotechnology Cultivation Systems from Discovery to Production 1 1 MiniBio 250 MiniBio 500 MiniBio 1000 Total volume ml 290 550 1000 Working volume ml 200 400 800 Minimum working volume ml 50 100 200 Aspect ratio total volume 2 3 2 1 2 3 Aspect ratio working volume 1 6 1 5 1 9 Dimensions dxh 180 x 400 mm 195 x 400 mm 200 x 550 mm Dimensions for autoclaving dxh 180 x 250 mm 195 x 250 mm 200 x 400 mm Drive system Direct drive lipsealed Direct drive lipsealed Direct drive lipsealed Maximum stirrer speed rpm 50 2000 50 1750 50 1500 Maximum impeller tip speed m s 2 3 2 0 3 5 Impellers Choice of Rushton and marine Gas sparger Gas overlay Porous sparger or L type sparger Yes Exhaust gas Electrically cooled exhaust gas condenser evaporation lt 4 per day at 37 C 2vvm Sampling Fixed sample pipe with optional sampling system Draining Height adjustable drain pipe Additions 4 fixed inlet ports and optional micro liquid injectors pH Measurement 8 mm classic pH sensor Control via acid pump variable speed pump or COs gas in combination with alkali pump variable speed pump DO Measurement 8 mm classic polarographic DO sensor Control via a combination of N2 Air O2 optional MFC and agitation or nutrient addition variable speed pump Temperature Measurement Pt 100 se
9. as pilot plant and production scale processes Your advantage Working with the latest technology offering a competitive advantage in efficiency and reliability Reliable As a privately owned company our focus is on building a healthy company by supplying the best product offering for our customers now and in the long term future This is the basis of many long term relationships with our Se clients all over the world Your advantage Applikon can always support you through the well trained sales consultants and technicians of our technical support department High Tech Applikon Biotechnology is unique in the mini and micro bioreactor range No other company can offer this complete solution on a very small scale We develop small scale systems to generate results that can be scaled to production scale There are thousands of Applikon bioreactors used in the world from small scale R amp D up to full E scale CGMP production Your advantage The best solution from micro scale to full scale production resulting in the shortest time to market for your new products Worldwide activities Since we started in 1974 we have shown a healthy growth resulting in a global market leader role R amp D design E and engineering are in house in our headquarters in Delft The Netherlands We have our own sales and service organizations in the USA the UK and China We have well trained local distributors for sales and service in over 20 cou
10. to 4 gas rotameters and up to 4 mass flow controllers for flexible gas supply for controlled liquid Autoclavable glass bioreactor Pump section with up to 3 easy access fixed speed pumps Emergency stop switches the system to safe position in case of emergency Connections for pH and DO Temperature and Foam Level Motor and Encoder motor Optional ADDA card for additional I O Connections for User friendly detachable and autoclavable bottle rack accommodates up to 3 liquid addition bottles or eee Temperature section for thermocirculator cooling water valve or heating blanket Quick release connections for addition condensor and heat exchanger ez Control Applikon Biotechnology Cultivation Systems from Discovery to Production 2 8 combination of ease of use and sophistication reducing the time to start up a process p p The system is equipped with the intelligent and powerful process controller easy and intuitive to operate such that the user manual might not be needed The process controller has control loops for oH Temperature Dissolved Oxygen Foam Level and agitation and can apply a combination of digital and analog outputs for ae rocess control Actuators such as rotameters solenoid valves mass flow controllers umps thermocirculator and others can be controlled The operator can set HI values dead band for pH control cascade control strat
11. very low in maintenance and very easy to use Features e Very user friendly laboratory device for monitoring anaerobic metabolic yeast activity for alcohol production e Six independent yeast fermentations can be carried out simultaneously e Stirrer speeds and temperatures can be set or time programmed for each fermenter flask individually e Accurate comparison of conversion rates and yields under different conditions such as temperature strain type carbon source or nutrients e Much easier to operate and cleaner than standard fermenter systems e Fully controlled with user friendly PC control and data analysis software e Automatic generation of advanced reports containing all measured data data analysis tables and corresponding graphs e Very strong magnetic stirrers able to handle very viscous media e Proven value for research and QA purposes in all fields of yeast research and bio ethanol production Applications e Measure conversion rates and yields of lignocellulose hydrolysates into biofuels e Test and compare different yeast strains or different feedstock substrates e Quality control of regular commercial yeast e Quality control of traditional feedstock such as molasses e Conversion of wort into beer e Conversion of grape juice into wine e Alcohol tolerance and toxicity studies e Industrial and academic research on yeast and potable alcohol bio ethanol bio butanol production Specifications Applikon
12. 15L 20L Working Volume 200ml 400ml 200ml 400ml 0 9L 1 7L 2 7L 0 9L 1 7L 2 7L 5L 10L 25L 800ml 800ml 3 2L 5 4L 12L 16L 3 2L 5 4L 12L 16L Agitator Lipseal with Lipseal with Lipseal with Lipseal with Mixing by marine impeller Rushton impellers marine impeller Rushton impellers rocking motion Aeration Air supply via Air supply via Air and Oxygen Air and Oxygen Air and Oxygen sparger sparger supply via sparger supply viasparger supply via overlay and overlay Exhaust gas Optional gas outlet Gas outlet Gas outlet Gas outlet condenser condenser condenser condenser Sampling Sample pipe Sample pipe Sample pipe Sample pipe Sample line included included included included included Sample system Sample system Sample system Sample system Sample system optional optional optional optional optional pH Measurement via Measurement via Measurement via Measurement via Measurement via pH sensor control pH sensor control pH sensor control pH sensor control pH sensor control via liquid alkali via liquid alkali or via liquid alkali via liquid alkali and via liquid alkali pump and CO2 acid addition pump pump and COs acid addition pump pump and COs gas supply gas supply gas supply Temperature Measurement via Measurement via Measurement via Measurement via Measurement via Pt 100 Pt 100 Pt 100 Pt 100 Pt 100 Heating only via heating blanket Heating and cooling via Peltier system Heating only via heating blanket Heat
13. East amp Africa UAE Alliance Global FZ LLC Phone 971 4 375 2744 www agbl net Isra l Medent LTD Phone 972 8 623 7984 www medent co il South Africa Separations Phone 27 11 919 1000 www separations co za Applikon Bioreactors V3X0120524 All rights reserved Subject to modifications Printed by Applikon Biotechnology by The Netherlands 2014 Co Ieselen BIOTECHNOLOGY Applikon Biotechnology BV Heertjeslaan 2 2629 JG Delft The Netherlands a step ahead E 31 0 10 208 35 05 E info applikon biotechnology com www applikon biotechnology com T 31 0 10 208 35 55
14. Pilot Microbial 140 100 20 3 0 2 2 Custom build bioreactor systems are available up to 2000 liter total volume Drive system Magnetically coupled optional mechanical seal bottom or top mounted agitator for microbial cultures and top mounted for cell cultere systems Maximum agitator tipspeed m s 5 m s for microbial cultures and 1 m s for cell cultures Impellers Rushton and marine with outside diameters 0 33 0 5 vessel diameter Gas sparger Porous sparger L Sparger or Ring type sparger Gas overlay Optional gas overlay line Exhaust gas Water cooled exhaust gas condenser with internal spiral and or jacketed Sampling Optional resterilizable sample system in DN25 port in lower side wall Draining Resterilizable bottom mounted bellows drain Additions Sterilizable additions push valves and resterilizable addition ports pH Measurement 12 mm classic pH sensor in DN25 port in lower side wall Control via acid pump or COz gas rotameter or MFC in combination with alkali pump DO Measurement 12 mm polarographic DOs sensor in DN25 port in lower side wall Control via a combination of N2 Air O2 Rotameter or MFC and agitation or nutrient addition pump Temperature Measurement Pt 100 sensor in in DN25 port in lower side wall Cultivation control cooling and heating jacket via bioreactor wall Foam Measurement Height adjustable conductivity based foam sensor Contro
15. TC diptube Perfusion outlet 3mm hosebarb 6mm hosebarb 6mm hosebarb 0 5 TC 0 5 TC Weight 0 1 kg 0 5 kg 1 5 kg 13 kg 70 kg Resonator volume 0 7 ml 7 mi 50 ml 290 ml 1450 ml BioSep Controller APS 995 APS 990 APS 990 APS 992 APS 993 BioSep Controller Power 15 Watt 150 Watt 150 Watt 350 Watt 1500 Watt BioSep Controller Dimensions Combined with 305x130x130 305x130x130 450x400x135 450x300x450 DxWxH mm my Control BioSep Controller Weight kg Combined with 3 5 3 5 17 6 43 my Control 46 Applikon Biotechnology Cultivation Systems from Discovery to Production Bench scale and Pilot plant steam in place bioreactor systems Applikon s concept of modularity using standard modules to customize the functions of SE the bioreactor is extended to the stainless steel pilot plant bioreactors as well For scale up purposes th e range of the Bio Bench and Pilot Systems designed and built to the latest standards on hygienic processing and cGMP and GAMP validation requirements complements the laboratory scale bioreactor systems Scale up from laboratory scale to pilot plant and small scale production is simplified by the consistent bioreactor design and E the scalable co ntrol solutions All systems are designed to be cleaned in place Applikon offers CIP systems ranging from fully manual contro to fully automated Standardized SN bioreactor systems are available up to 140 liter total volume and cust
16. ance Downtime and emergency call outs can be costly and always happen unexpectedly during an important run Preventive maintenance extends the lifetime of the major components of your system preventing premature wear and failure Benefits of Applikon s service agreements e Reduced charges for emergency repairs e Free access to firmware upgrades SE e Reduced labour charges for any upgrade work or refresher training on site Extended warranty This plan is a two year extension of the original equipment warranty and includes parts and labor as needed Instrument calibration service e Validation e Calibration certification Corporate Office Netherlands Applikon Biotechnology B V Phone 31 10 208 3555 www applikon biotechnology com America USA Canada Applikon Biotechnology Inc Main US Offices Phone 1 650 578 1396 www applikonbio com Applikon Biotechnology Inc East Coast Office Phone 1 973 361 5387 Mexico Applikon Biotechnology Mexico Phone 52 55 5868 7814 Brasil Alem Mar Phone 11 322 983 44 www alemmar com or Europe Belgium Applikon Biotechnology B V Phone 31 10 208 3555 www applikon biotechnology com Denmark Holm amp Halby A S Phone 45 432 694 00 www holm halby dk France System C Industrie Phone 33 475 548 600 www system c industrie com Germany IUL Instruments GmbH Phone 49 222 391 920 www iul ins
17. ariable speed pumps or micro addition valves for extremely accurate nanoliter volumes e Enhanced gas addition strategies via 3 mass flow controllers e No water connections needed due to electrical cooling and heating system for bioreactor and condenser e Expandable control system with optional extra inputs and outputs e USB connection for optional balances Biomass or Fluorophor pH and DO sensors _ Applications e Ideal for screening media optimisation modelling toxicity studies etc e Mini bioreactors e Autoclavable bioreactors up to 3 liter volume e Single Use Bioreactors up to 3 liter volume e Microbial and Cell cultivation e Batch Fed Batch Perfusion and Continuous cultivation Applikon Biotechnology Cultivation Systems from Discovery to Production 2 a Specifications Inputs pH temperature dissolved oxygen agitation foam and level Optional biomass and fluorophor pH and fluorophor dissolved oxygen Optional inputs 4 x analog in 0 10 V Control type PID control with selectable autotuning adaptive control Actuators Up to 6 digital variable speed pumps or micro addition valves for addition of extremely accurate nanoliter volumes Up to 3 Mass Flow Controllers or 4 solenoid valves for gas flow control Electrical bioreactor heating via Peltier element or Heating Blanket Electrical bioreactor cooling via Peltier element Electrical condenser cooling via Peltier element
18. ase in product expression e Up to 6 fold increase in Oxygen Transfer Rate e Up to 5 times shorter cultivation time e Humidity control Shake flask under the influence of ResonantAcoustics Specifications Capacity 18 x 250 ml flasks or 14 x 500 ml flasks or 8 x 1000 ml flasks Dimensions 102 x 57 x 70 cm W D H AO x 27 x 23 Temperature control Ambient 5 C to 60 C Weight 200 kg 440 lbs Humidity control Ambient to 90 RH Power 110 230 VAC 50 60 Hz Mixing power 3 20g acceleration 196 m sec O 8 Applikon Biotechnology Cultivation Systems from Discovery to Production Anaerobic Fermentation Monitor Simple parallel cultivation The Anaerobic Fermentation Monitor or AFM is a robust and user friendly laboratory parallel fermentation system that allows for accurate comparisons of carbon conversion 7 rates and yields for six simultaneous anaerobic fermentations Monitoring the amount of gas that evolves from a fermentation broth under well controlled a conditions is a reliable comparison method that has proven to be very useful in all industries that use anaerobic fermentations Because carbon dioxide production is stoichiometrically coupled with carbon source conversion very useful metabolic data can be obtained The AFM provides very accurate standard measuring of gas from six parallel fermentations that is both
19. ate underlines the high approach is a customized bioreactor system that is delivered on quality level of our work and accompanying documentation time and completely according to the customer s specifications KO Applikon Biotechnology Cultivation Systems from Discovery to Production AppliCare total process control Sch What separates our service engineers from other organizations is that our people are BioProcessing Specialists This means that not only do we know how to maintain and repair equipment we can also troubleshoot because we understand process control We specialize in bioreactors controllers software motors probes and all phases of your a specific system s operation Applikon s AppliCare will fix your problems guaranteed We help to eliminate the lost hours incurred to your production schedule and the high costs of downtime Installation and training A correctly installed system with comprehensive on site training minimizes set up time and ensures optimum operation within the shortest timeframe The installation service incorporates e Pre plan design amp Supply Service Requirement over view before arrival of _ Applikon Equipment into your lab ee e Connection of all tubing hoses or piping from the Applikon Control Skid to the Supply Services or the Utility Panels e Installation of all cabling from SCADA software to the controllers Es Site acceptance tests Preventive mainten
20. ce IP 30 and IP 65 for Control Panel and IP67 for Light bank 16 x 2 Backlit LCD Feather touch keys Full duplex RS232 or RS485 interface optional 0 C up to 60 C 115 230 V AC 50 60 Hz Maximum 150 W The PhotoBio range of bioreactors consist of the following products PhotoBioSTR2 PhotoBioSTR3 PhotoBioSTR5 PhotoBioSTR7 PhotoBioSTR15 PhotoBioSTR20 PhotoBioSU10 PhotoBioSU20 PhotoBioSU50 5 liter stirred tank bioreactors single wall non jacketed 15 liter stirred tank bioreactors single wall non jacketed 20 liter stirred tank bioreactors single wall non jacketed 120 Wat 10 liter single use AppliFlex rocking bioreactors 40 Wat 20 liter single use AppliFlex rocking bioreactors 60 Wat 50 liter single use AppliFlex rocking bioreactors 2 liter stirred tank bioreactors single wall non jacketed 40 Wat 3 liter stirred tank bioreactors single wall non jacketed 40 Wat pane pane 60 Watt pane 7 liter stirred tank bioreactors single wall non jacketed 60 Wat pane 120 Watt pane pane pane pane 120 Watt pane 1 8 Applikon Biotechnology Cultivation Systems from Discovery to Production single use bioreactors Applikon offers several types of single use bioreactors AppliFlex rocking bioreactors from 10 to 50 liten HyClone SUB stirred tank bioreactors in the range of 66 to 2 700 liter total Sek volume ATMI PAD square bioreac
21. cells Applikon Biotechnology Cultivation Systems from Discovery to Production 2 1 Specifications Se AppliFlex 10 25 50 z E b Total volume L 10 25 50 Ac Max working volume L 5 12 5 25 Min working volume L 2 5 10 A Hyclone 50 100 250 500 1000 2000 Total volume L 66 120 316 660 1320 2700 Max working volume L 50 100 250 500 1000 2000 Min working volume L 25 50 125 250 500 1000 Pad Mini 25 50 125 250 600 1200 Total volume L 16 25 50 125 250 600 1200 SE Max working volume L 13 20 40 100 200 480 960 oO Min working volume L 3 2 8 10 25 25 120 240 a iCellis Nano a al Volume L om 008 oz A Surface area Low m2 0 53 1 06 2 6 Surface area High m2 0 8 1 6 4 Em Sensors Fluorophor pH Fluorophor Gel filled Sterilizable Temperature Dissolved sterilizable pH Polarographic Oxygen Dissolved Oxygen Measuring range 5 5 8 5 pH 0 100 0 12 pH 0 100 0 150 C O2 saturation O2 saturation Accuracy 0 1 pH 0 4 0 1 pH 2 0 1 C Measuring temperature range O 40 C 0 40 C 0 80 C O 60 C 0 150 C Maximum temperature 45 C 45 C 135 C 135 C 150 C Sensor life time 2 weeks 6 months gt 1 year gt 1 year gt 1 year 1 measurement per minute 3 5 weeks 1 measurement per 2 minutes Features amp Applications _ e Simple operation e Fully documented delivery eases validation e OPC compliant controller e One point of contact for the complete s
22. controller for small scale bioreactors starting a ii Se a rr T d t 50 ml working volume The system can control bioreactors up to a total volume of 3 L his versatile controller can be used for both cell culture and microbial cultures he advanced software makes it possible to switch from microbial to cell culture configuration in seconds With its footprint of only 19 by 35 cm W x D it uses the minimal mount of bench space allowing to set up as many as 5 on 1 m width of bench space he built in web server allows the my Control to be operated by any computer with a web browser Internet Explorer Mozilla Firefox Apple Safari or Google Chrome Wireless evices like iPad iPhone or Android tablets or phones can also be used to operate my Control The selectable colored band on the unit allows the system to be personalized al nd to fit your laboratory eg Features e Control of all bioprocess parameters including pH temperature dissolved oxygen agitation foam and level e Selectable autotuning adaptive PID control for accurate control when process conditions change during the culture e Easy operation through web browsers Windows PC Apple PC Linux PC iPhone iPad Android tablet Android phone e Parallel processing is enhanced by allowing up to 32 my Controllers in one human interface 1 e Extended liquid additions options tuned to small scale cultivation via up to 6 digital v
23. egies and dose monitors for liquid additions The advanced auto tuning adaptive control system is part of every bundle and takes the guessing out of PID controller setting The system can automatically and continuously calculate the best controller settings for every process Classical pH and DO sensor port Gas in and Sample port Drain port Electrically cooled outlet lines condensor Accurate agitation Quick release topplate clamp Additions A 7 4 i pH and DO sensors Sample Agitator with rushton or Fluorophor pH marine impellers and DO sensor port AppliFlex MiniBio 2 4 Applikon Biotechnology Cultivation Systems from Discovery to Production Modularity and configurability Although the BioBundle has a pre set configuration the whole bioreactor system remains modular and configurable With simple changes in configuration the BioBundle system can be modified for different applications in a cost effective way This applies to changes in process control strategies in using extra gas or liquids but also in changing the system into a microbial or cell culture set up Compact design The BioBundle is designed to occupy as small a footprint as possible All accessories such as pumps and gas flow control valves are conveniently integrated in a compact console The compact design Ee reduces the need for expensive lab space Software for data acquisition and supervisory cont
24. equals Contact Low Conductivity gt 200 uS equals Contact 2 Channel analog inputs Range 4 20mA Accuracy 0 1 Full Scale value ES 2 Channel analog outputs Range 4 20mA SE Accuracy 0 1 Full Scale value 2 Channel analog inputs for RTDs Sensor Type Pt 100 EH Range 200 C 800 C GR Accuracy 0 2 Full Scale value OO Resolution 0 1 C oD a 1REatseItsttb K ell LED LE 8 4 Applikon Biotechnology Cultivation Systems from Discovery to Production Bioxpert Supervisory control and data aquisition Bioxpert is a software too to control bio processes and to store the process data The software is designed specifically for biotech researchers and operators in the Ee bio environment The logical structure of the program and the user friendly interface allows the program to be used by anyone That is why over 3 000 copies of our Bioxpert software a are in use worldwide The software is easy to operate and has extensive functionality for monitoring collecting and storing data and controlling processes Various BioXpert versions have been developed based on international standards for use in either research and pilot plant or production environments BioXpert Lite is designed for data collection data storage and data presentation The research version BioXpert 2 offers maximum flexibility to set up and to modify processes The pilot plant and production
25. ertips The ez Control accurately controls pH Temperature Dissolved Oxygen Foam Level and SEN Agitation in bioreactors and fermentors The color touch screen interface guides the user through the intuitive operation The adaptive control features allow the user to focus on the process while the controller keeps tight control on the important process parameters What makes this controller unique are its ease of use its small footprint its flexibility and its accurate control The optional ADDA board offers an additional 8 analog inputs E 4 analog outputs 8 digital inputs and 8 digital outputs Features e Simple operation e Adaptive PID control e OPC compliance e e User definable controlloop configuration E e Store copy and reload process configurations e Local data storage and display up to 72 hours Se e Synoptic trending and bar graph displays e Multiple user levels EF Applications e Autoclavable bioreactors e Steam in Place bioreactors e Single Use Bioreactors e Microbial and Cell cultivation e Batch Fed Batch Perfusion and Continuous cultivation cKO WAAT Tage D Specifications Measurement range Measurement accuracy pH 0 14pH 0 01 pH Temperature 0 150 C 0 1 C Dissolved Oxygen O 100 saturation 0 1 Foam Level Conductivity based measurement On off control Agitation O 1250 rom 1 rom O
26. eutical industry it is vital to have precise information about the Sg OH value of the bioprocess The pH level directly affects viability productivity stability of ch the cells and it influences analysis of active ingredients The AppliSens HIT sensor is specifically designed for long term stable and accurate measurements in bioprocesses The pH t sensor has a fixed sleeve diaphragm that reduces the influence of the culture medium on the pH measurement s Compared to classic diaphragm types the sleeve diaphragm increases measuring accuracy and increases the lifetime of the sensor which is vital for long lasting biotech cultivation processes Features e Robust design by using tempered glass shaft e Sensor head uses minimal space on bioreactor topplate e Accurate measurement e Low sensitivity to fouling due to sleeve diaphragm e Stable signal over longer time e FDA approved materials e Withstands repeated SIP and CIP cycles e Standardized PG 13 5 connection guarantees interchangeability with other bioreactor brands Applications e Microbial cultures e Cell cultures e Batch e Fed Batch e Continuous cultures e Perfusion cultures Applikon Biotechnology Cultivation Systems from Discovery to Production 4 1 Specifications Parameter As Delivered DR pH range 0 12 pH Temperature range 0 135 C Electrode zero point E7 15 mV K
27. h 1 3 VVM 75 humidity depth 40 mm 2400 pi 11 ul H O per day 1000 yl 300 rpm 50 mm 18mmolO2 1 h 1ml min beem 750 il 1000 pl 300 rpm 25 mm 3 mmolO2 1 h 1 WM H H 100 ul 300 rpm 50 mm 39mmolO 1 h 250 pl min 96 round low well C 100 ul 300 rpm 25 mm 20 mmol O 1 h 2 5 VVM 50 humidity i olystyrene i S GER eat 150 ul 300 rpm 50mm 82mmol0 I h 250pl min Du HO per day i S 150 pl 300 rpm 25 mm 16 mmol O 1 h 1 7 WM 75 humidity depti mn 3 uI Ha per day 150ul 150 ul 200 ul 220 rpm 50 mm 12 mmol O 1 h 250 pl min 200 ul 300 rpm 25 mm 12 mmol O 1 h 1 3 VVM Various configurations of cover clamps and sandwich covers CG Applikon Biotechnology Cultivation Systems from Discovery to Production RAMbio the high intensity mixer The RAMbio bench top biological mixer utilizes ResonantAcoustic technology that applies low frequency high intensity acoustic energy to agitate microbial cultures The RAMbic is a highly efficient mixer incubator that dramatically enhances oxygenation and bulk mixing for bacterial and fungal cultures Combined with the patented Se Oxy Pump stopper oxygen transfer rates up to 6 fold higher than orbital shaken cultures can be achieved oo Features e Increased oxygen transfer for aerobic cultures e Rapid turnover of any desired gas e Take advantage of richer media formulations e 2 6 fold increase in plateau biomass levels e Up to 10 fold incre
28. idation Applikon Biotechnology Cultivation Systems from Discovery to Production 1 9 HyClone S U B The HyClone S U B provides all the advantages of single use bioprocessing in a classical stirred tank bioreactor system design The critical design parameters of the S U B such as height to diameter ratios mixer design and location and typical control system interfaces have been maintained The S U B is available with new optimized standard BioProcess Containers with improved dual sparge capability Open pipe and frit sparging systems are included to provide a wider range of operating conditions A key element to the single use design is the plastic polyethylene impeller with a bearing seal assembly linked to an external mixer drive The range includes units with maximum working volumes of 50 100 250 500 1 000 and 2 000 L 20 Applikon Biotechnology Cultivation Systems from Discovery to Production ATM iCELLis Nano The ATMI iCELLis Nano system is designed to simplify your process by combining the advantages of single use technologies with the benefits of a fixed bed system Central to the iCELLis bioreactor technology is the use of a compact fixed bed filled with macrocarriers This matrix is made of medical grade polyester microfibers and provides up to 4 m2 available area for cell growth in only 200 ml volume This is the surface equivalent of 47 roller bottles The iCELLis bioreactor is pr
29. ing device specifically developed for analysing Sg glucose and lactate in bioreactors Patented heat flux technology is used for continuous ech measurement with high precision and repeatability The combination of the BioSenz with a process contro system allows accurate control of the analyte levels in a bioreactor to optimize the performance of the bioprocess The operation of the analyzer can be done through the process control system or via a computer using the BioSenz software eg Features Heat flux technology e Accurate monitoring and control of glucose and lactate Enzymatic conversion of glucose to gluconic in your bioprocesses acid and water or lactate to puruvate and water RE e Single use sensor which is directly proportional to the concentration e Compact footprint of glucose in the sample The BioSenz measures a e Stable biosensors up to 4000 measurements the heat flux from the reaction and this patented e Reliability due to unique patented heat flux measuring technology approach provides a highly accurate highly e Connection to re usable and single use reactors specific determination of glucose and lactate eg e Automatic calibration in your bioreactor e No sample preparation Applikon Biotechnology Cultivation Systems from Discovery to Production 43 Concentrations measured in presence of other System setup medium compounds in different concentrations e The sterilizable and autoclavable sample probe
30. ing via heating blanket cooling by cold water in heat exchanger Heating only via heating blanket Dissolved Oxygen Measurement via DO sensor control via Air and O2 gas supply Measurement via DO sensor control via Air supply and agitation speed Measurement via DO sensor control via Air and O2 gas supply Measurement via DO sensor control via Air and O2 supply and agitation Measurement via DO sensor control via Air and O2 gas supply speed Foam Option Measurement via Option Measurement via Foam sensor Foam sensor control via anti foam control via anti foam addition pump addition pump Level Option Option Option Option Liquid additions 4 fixed in topplate and 1 septum port 4 fixed in topplate and 1 septum port 3 ports in triple inlet and one inoculum port 3 ports in triple inlet one inoculum port 1 and septum port One inoculum and alkali inlet One liquid storage system included Two liquid storage systems included One liquid storage system included Three liquid storage systems included One liquid storage system included Start up kit Included Included Included Included Included BioXpert Lite software Included Included Included Included Included 26 Applikon Biotechnology Cultivation Systems from Discovery to Production my Control color your lab my Control is the most advanced bioreactor
31. is used OmMglucose 4M glucose 20 mM glucose to take a cell free sample from the bioreactor The system has integrated buffer solutions and calibration standards Fructose 2mM lt 0 3 mM 1 07 mM 19 2 mM to minimize the footprint The auto calibration function Maltose 2mM lt 0 3 mM 1 04 mM 19 9 mM a Ke EE reduces operator involvement and increases the reliability Lactose 2mM lt 0 3 mM 1 02 mM 19 5 mM i Kg of the measurements The optional multiplexer allows Sucrose 2mM lt 0 3 mM 1 00 mM 19 7 mM E the system to be connected to up to four bioreactors Serum Dot lt 0 3 mM 0 96 mM 20 0 mM i E simultaneously Analog outputs are used to connect the Yeast ext 2 g l 19 6 mM 3 analyzer to a process control system The sensor chip No interference in lactate measurement was detected by different determines the analyte to be measured At the moment concentrations of ascorb acid acetaminophen paracetamol and uric acid we offer glucose and lactate analysis Batch Cell Culture Analysis Technical data en i Gl L St 7 S S BioSenz ucose actate 6 KM See A Reference Measurement principle Enzymatic heatflux be gt MM S 5 aes Measurement range g l 0 05 8 0 05 2 5 es 8 4 a Measurement deviation lt 3 lt 3 Q g 3 Measurement frequency Up to 10 per hour 2 8 i W Active sensor life 30 days or gt 4000 samples 5 1 SR i o 0 Sensor
32. l Anti foam addition pump Level Measurement Height adjustable conductivity based level sensor or loadcells in bioreactor frame Control pump for liquid addition or removal Applikon Biotechnology Cultivation Systems from Discovery to Production AQ Special projects Customized solutions Some customer demands cannot be fulfilled with our standard product offerings In these cases we build custom solutions for our customers These projects can range from very special mini bioreactors to a complete line of CGMP production systems ranging up to several thousand liters volume systems Em L 1500 Liter Bioreactor with i Control Key to the success of these projects is our experience of Over the past years we have generated a large library of P over 35 years building customized bioreactor solutions in customized solutions for a variety of specific demands combination with clear communication between our project This guarantees that we can supply our customers with a Z team and the customer We have developed special web based proven solution for virtually any process demand tools for communication and open information exchange within Since we are standardized on supplying products for the the project team This ensures that everybody is working with pharmaceutical industry all our products and processes are the same up to date information all the time The result of this fully documented Our ISO 9001 certific
33. mple pipe internal diameters choices are 1 7 mm 4mm 6 mm or 10 mm Draining Drain pipe Additions Triple or single inlet ports and optional micro liquid injectors pH Measurement 12 mm classic pH sensor Control via acid pump or CO gas rotameter or MFC in combination with alkali pump DO Measurement 12 mm classic polarographic DOs sensor Control via a combination of No Air O2 Rotameter or MFC and agitation or nutrient addition pump Temperature Measurement Pt 100 sensor in thermowell in topplate Control cooling and or heating jacket via bioreactor wall or via internal heat exchanger Foam Measurement Height adjustable conductivity based foam sensor Control Anti foam addition pump Level Measurement Height adjustable conductivity based level sensor Control pump for liquid addition or removal Optional inlets Septum chemostat tube liquid entry system 1 6 Applikon Biotechnology Cultivation Systems from Discovery to Production Photo bioreactors the next best thing to sunlight The Applikon PhotoBio photobioreactor range is based on the standard Applikon SE re usable and single use bioreactors Special light panels are added to these systems to allow the growth of photosynthesizing organisms plants algae bacteria The well proven design of the bioreactors in combination with the state of the art LED 4 light panels guarantee the best performance of the PhotoBio for any application The light
34. nsor in thermowell in topplate Control electrical cooling and heating jacket via bioreactor wall Foam Measurement Height adjustable conductivity based foam sensor Control Anti foam addition variable speed pump Level Measurement Height adjustable conductivity based level sensor Control variable speed pump for liquid addition or removal Optional inlets Optional sensors Septum chemostat tube liquid entry system Biomass Optical Density O2 and CO off gas cee Se E x A Applikon Biotechnology Cultivation Systems from Discovery to Production 1 8 Glass autoclavable bioreactors the world wide standard In the laboratory bioreactor and fermentor range Applikon is worldwide market leader because of its dependable and easy to use systems The bioreactors and fermentors excel in quality and modularity An Applikon laboratory system is easy to upgrade if a change in research activities occurs Applikon offers glass autoclavable bioreactors for cell culture applications and glass autoclavable fermentors for microbial culture applications The systems are built according to the specific demands of a process using an extensive array of standard components Because of the modularity and flexibility the user can always adapt the systems to changed process demands This results in low initial investment and low running costs The stirred tank reactor STR is the most widely used bioreactor type Glass au
35. ntries Your advantage No matter where you are you can always rely on Applikon now and in the future Our Mission Providing reliable solutions for the bioprocess market that will enable an improved quality of life O cooikon BIOTECHNOLOGY a step ahead _ O 4 Applikon Biotechnology Cultivation Systems from Discovery to Production Micro Flask by duet Cultivation in microtiter plates The Micro Flask system facilitates reproducible and reliable culturing on microtiter plates The system consists of sandwich covers cover clamps and cryo replicator The Micro Flask enables a single person to grow and test thousands of strains simultaneously with a minimum of repetitive handling Cryo Replicator for simple inoculation of 96 well microtiter plates _ Features e Conversion of 24 and 96 microtiter plates both deep and low well plates into individual micro reactors e Low and uniform evaporation rates for every well e Sterile barrier for individual wells prevents cross contamination e Oxygen transfer rates similar to shake flasks in standard orbital shakers e Simultaneous and reproducible sampling of 96 frozen glycerol stocks Applications e High throughput screening and distribution of mutant and construct CULTURE Ree libraries e g in E coli or yeast e Metabolic flux studies and high throughput screening for high activity Cross section of Micro Flask prokaryotic
36. om build units can be supplied up to 2 000 liter total volume Features to allow efficient e Modular design e Magnetically coupled agitator for peace of mind e cGMP compliant design simplifies validation e Compact design reduces floor space needed e Open frame construction gives easy access for maintenance and operation e Easy to clean mirror polished external finish e Electropolished finish of all parts in contact with the culture Ra lt 0 4 um clean in place allows easy adaptation to changing process demands Applications e Scale up studies and Continuous e Medium optimization e Process optimization e Small scale production e Microbial and Cell culture e Batch Fed Batch Perfusion cultivation 15 Liter BioBench with ez Control Specifications Applikon Biotechnology Cultivation Systems from Discovery to Production 4 P Total volume Working Minimum Aspect Aspect liter volume liter working ratio total ratio working volume liter volume volume 7 liter Bio Bench 7 5 2 22 1 5 15 liter Bio Bench 15 10 4 2 1 1 4 20 liter Bio Bench 20 15 4 3 0 2 2 30 liter Bio Bench 30 22 5 7 2 0 1 6 30 liter Pilot Cell 30 20 7 5 1 5 1 0 60 liter Pilot Cell 60 40 10 1 6 1 0 130 liter Pilot Cell 130 100 28 1 5 1 0 20 liter Pilot Microbial 20 15 4 3 0 2 2 40 liter Pilot Microbial 40 30 15 3 0 2 2 70 liter Pilot Microbial 70 50 10 3 0 2 2 140 liter
37. or eukaryotic mutants sandwich cover e Comparative studies e g clinical isolates e Growth medium optimization for cell lines or production strains Specifications Applikon Biotechnology Cultivation Systems from Discovery to Production O 5 Mixing pattern Type of Well volume Culture Orbital Shaking O transfer rate Headspace Evaporation at 300 rpm microtiter plate volume shaking amplitude 30 C air 1 bar refreshment rate per well ampl ampl frequency rate at 30 C 25mm 50 mm 24 square deep well 2500 ul 300 rpm 50 mm 51 mmol O 1 h 2 5 ml min polypropylene 2500 ul 300 rpm 25 mm 39 mmol O 1 h D VVM 50 humidity 17x17 mm 11000 ul 2500 ul 220 rpm 50mm 28 mmol Oe ID 50 ul H 0 per day depth 40 mm 75 humidity 4000 ul 300 rpm 50 mm 24mmolO 1 h 2 5 ml min 25 ul H O per day 4000 ul 220 rpm 25 mm 24mmolO 1 h 0 6 VVM 2500 pl 2500 pl EH 750 ul 300 rpm 50 mm 40 mmol O 1 h 1 1 ml min 50 humidity polystyrene 750 ul 300 rpm 25 mm 25 mmol O 1 h A VVM 30 pI H O per day Wl 16 mm 1000 yl 300 rpm 50mm 830mmolO I h 14 1ml min 75 humidity Sg depth 18 mm 4000 yl 300 rpm 25mm 19mmolO 1 h 1 1 VVM 15 ul HO per day 4000 ul 1000 pl 500 ul 300 rpm 50 mm 38 mmol O I h 1ml min 96 square deep well 500 ul 300 rpm 25 mm 12 mmol O 1 h 2 VVM 50 humidity olypropylene GEN 750 ul 300 rpm 50 mm 24 mmol O 1 h 1 ml min 22 ul H20 per day 750 yl 300 rom 25 mm 7ZmmolO 1
38. ovided with pre packed macrocarriers Evenly distributed media circulation is achieved by a built in magnetic drive impeller ensuring low shear stress and high cell viability The cell culture medium flows through the fixed bed from the bottom to the top At the top the medium falls down the outer wall as a thin film where it takes up Oo to maintain high ku a the in the bioreactor iCELLis nano Fixed bed height 10 cm e Fixed Bed Volume 02L e Culture Surface Area 4 m2 e Equivalent CS10 6 300 cm2 6 e Equivalent RB 850 cm 47 mm AIMI PadReactor The ATMI PadReactor system is perfectly suited to laboratory environments process development centers clinical material supply and flexible GMP manufacturing The square bioreactor vessel which offers comparable functionality to classical stirred tank bioreactors is a single use bag integrating an internal paddle mixing and sparger system The sparger is integrated in the paddle mixer to enhance oxygen transfer rates The innovative bag design improves mixing at low shear forces and allows a non invasive stirrer connection to the system The paddle is enclosed in a medical grade ULDPE sleeve made from the same contact material as the bag itself and is coupled on top of the vessel with the mechanical mixing head The PadReactor is available in total volumes of 25L 50L 250L 600L 1200L and has been used successfully in cultivation of anchorage dependent and suspension
39. p can be applied in both R amp D max 1 L day process development and on production scale 1000 L day Sa a MrFFT I be ene ri eel pu gigas Sore Applikon Biotechnology Cultivation Systems from Discovery to Production 4 5 Typical configuration of acoustic cell retention system CLARIFIED CULTURE MEDIUM FRESH FEED CONCENTRATED CELL RECYCLE HARVEST CELL SUSPENSION CELL SETTLING Features e Proven under cGMP conditions e Easy to install and to operate Applications e Cell cultures e Perfusion cultures e Can be used in combination with any brand of bioreactor e Long term cultivation possible gt 6 months e No fouling or blocking e No damage to the cells e High separation efficiency gt 95 Specifications BioSep system 1L 10L 50L 200L 1000L Maximum perfusion rate 1 L day 7 Uday 45 L day 200 L day 1000 L day Minimum perfusion rate 0 1 L day 0 7 L day 4 5 L day 20 L day 100 L day Separation efficiency Up to 99 depending on cell concentration and perfusion flow Minimum cell concentration 2 x 108 cells ml depending on cell size Bioreactor connection 6mm OD tube 12mm OD tube Mounted Mounted Mounted separately separately separately Medium inlet 3mm hosebarb 6mm hosebarb 10mm hosebarb 0 5 TC 0 5 TC Return line to bioreactor 3mm hosebarb 6 mm OD 0 5 TC 0 5 TC 0 5
40. r process optimization and allows to maximize the product yield A microorganism or cell responds to the oxygen concentration in regulating its overall metabolism Therefore the knowledge of the DOs concentration and the proper control during the process are of great importance The AppliSens DO2 sensor is specifically designed for long term stable and accurate measurements in bioprocesses Se The DOs2 sensor has a titanium membrane module to minimize the measurement drift The autoclavable polarization module allows polarizing of your DO2 sensor while autoclaving your bioreactor system resulting in reduced start up time for your culture Features Applications _ e Titanium and PEEK materials guarantees low drift e Microbial cultures e cGMP compatible by using FDA approved materials e Cell cultures SE e Electropolished surface roughness finish of 0 4 um e Batch eases cleaning e Fed Batch ER e Short response time e Continuous cultures e Withstands repeated SIP and CIP cycles e Perfusion cultures e Standardized PG 13 5 connection guarantees interchangeability with other bioreactor brands Specifications Cultivation Systems from Discovery to Production 8 9 Optimum polarization potential 800 mV 40 C Polarization potential amplifier 675 mV Max pressure where linearity is guaranteed 4 bar Max sterilization temperature 135 C Minimum polari
41. r set up as well as in a Quad 4 bioreactors configuration This standard control solution can be supplied with Se Allen Bradley or Siemens PLC s Basic functionality includes advanced process control for numerous parameters fully automatic sterilization and Clean In Place routines The off the shelf system is supplied pre configured and ready to use Features e Off the shelf standard Solution e Reliable control platform Allen Bradley and Siemens hard and software e Supplier independent solution based on industry standard hardware e Local Control and local display e Integrates with any factory automation system e Cost effective e Fully documented supply eases validation a e Can be used in a 21CFR part 11 compliant system Applications E e Laboratory scale bioreactors e Pilot plant scale bioreactors e e Production scale bioreactors e Microbial cultures n e Cell cultures wera Applikon Biotechnology Cultivation Systems from Discovery to Production 8 8 Specifications SS pH Temp input pH amplifier Range O pH 14 pH Accuracy 0 01 pH ER Temperature amplifier Type Pt 100 3 wire type Range OC 150 C Accuracy 0 1 C DO Level input DO amplifier Type Polarographic Range 0 500 air Accuracy 0 1 Level amplifier Type On Off signal Sensitivity Software selectable H High Conductivity gt 26 uS
42. rol The BioBundle control system includes an Ethernet connection port to connect to a PC with software for data acquisition or SCADA such as the Applikon BioXpert packages BioXpert Lite data acquisition is included in this BioBundle Multiple bioreactors can be connected to one PC for data acquisition Optional BioXpert packages are BioXpert 2 and BioXpert W7 SCADA of multiple bioreactors including cGMP production applications Cost Saving The BioBundle is a pre packed system The assembling of the BioBundles in series provides a cost saving which is for the benefit of the customer Summarising Over all some of the benefits Applikon bioreactors provide include e dependable and reliable operation greater productivity and yield e modular design less cost in the future Z being able to use same equipment for different applications e less downtime due to maintenance greater economy in operation higher yield to cost ratio n e simple operation less operator training required Specifications Applikon Biotechnology Cultivation Systems from Discovery to Production 2 5 MiniBioBundle MiniBioBundle BioBundle BioBundle AppliFlex Cell Culture Microbial Cell Culture Microbial BioBundle Control system my Control my Control ez Control ez Control ez Control Total Volume 250ml 500 ml 250ml 500 ml 1L 2L 3L 54L TL 2 SL SL 10L 20L 50L 1000ml 1000m 7L 15L 20L 7L
43. shelf life gt 6 months at 2 8 C T T T ji BEEN 0 20 40 60 80 Communication interface USB and analog output option Cultivation Time h Maximum number Up to 4 with optional Multiplexer reactors per analyzer E Lactate and Glucose L Plantarium Fermentation Dimensions HxWxD 33 x 15x 360m 13x6x 14 35 Weight kg 6 5 30 4 tet Ni narant D pas eee nage 25 ty om ES 20 Ze ue Lactate 15 A o v Glucose 10 L mM D 5 D nanna EN Ee HIT 0 aag terte wr verte 0 5 10 15 20 25 30 35 40 45 Cultivation Time h Barc OTD eaa Legs Cell Culture with Glucose Feeding 3 0 2 5 2 0 a m Biosenz 1 5 s ef a Reference 10 Lactate Concentration g l 0 5 at A grune ER SET 0 0 Ap naddo ar gepeg 0 5 10 15 20 25 30 35 40 Cultivation Time h 4 4 Applikon Biotechnology Cultivation Systems from Discovery to Production Blosep acoustic cell retention system The Applikon BioSep system is a unique cell retention device for high density perfusion processes Using high frequency resonant ultrasonic waves to separate cells instead of a a physical mesh or membrane it offers all the benefits of traditional devices but without their inherent problems and limitations The BioSep based on the technology of acoustic Ss resonance is a non fouling non clogging retention system The BioSe
44. sta Windows 7 32 bit 2000 XP Vista Windows 7 32 bit Vista Windows 7 32 bit Communication drivers include ADI 1010 ADI 1020 ADI 1030 ADI 1010 ADI 1020 ADI 1030 ADI 1060 ADI 1010 ADI 1030 ADI 1060 my Control my Control my Control via OPC ez Control ez Control ez Control via OPC in Control in Control in Control via OPC i Control via OPC AppliFlex Rocker AppliFlex Rocker AppliFlex Rocker Balances Balances Additional drivers available separately no no yes ADDA card ADDA card Measurement of process values from process controllers yes yes yes Control of process setpoints in process controllers no yes yes Starting and stopping of control loops no yes yes Programming of local process controllers no no yes Reading of calibration data no no yes 21 CFR part 11 compliant no no yes GAMP compliant yes yes yes ISA S88 standard no no yes Password protection no no yes User definable access rights no no yes Auto start after power failure no no yes BioXpert Lite QOON we E SCHEID ZK CN Se N h BioXpert 2 oo a feds dere UK BioXpert W7 iroot ETEF AKTAD Specifications Applikon Biotechnology Cultivation Systems from Discovery to Production 8 S Specifications BioXper
45. t Android phone e Enhanced gas addition strategies via up to 4 Mass Flow Controllers GER e Expandable control system with optional extra inputs and outputs Sg e USB connection for optional biomass or fluorophor pH and DO sensors and balances e Ethernet communication to SCADA Applications e Replacement of ADI 1010 Bio Controller and ADI 1030 Bio Controller EE e Autoclavable bioreactors up to 20 liter volume E e Single Use Bioreactors SE e Microbial and Cell cultivation oe e Batch Fed Batch Perfusion and Continuous cultivation Specifications Applikon Biotechnology Cultivation Systems from Discovery to Production 2 9 Inputs pH temperature dissolved oxygen agitation foam and level Optional biomass and fluorophor pH and fluorophor dissolved oxygen Optional inputs 4 x analog in 0 10 V Control type PID control with selectable autotuning adaptive control Actuators 8 x digital outputs for heating cooling fixed speed pump solenoid valves etc Up to 4 Mass Flow Controllers or 4 solenoid valves for gas flow control bere Spare WO 4 x analog out 0 4 20 mA Additional features Emergency stop connection central alarm output lt Power Pmax 480 VA 230 115 Vac Se Dimensions dxwxh 322 x 312 x 210 mm _ Weight kg 7 2 kg 30 Applikon Biotechnology Cultivation Systems from Discovery to Production ez Control total control at your fing
46. t Lite BioXpert 2 BioXpert W7 Data management On line data collection yes yes yes Off line data collection yes yes yes Data storage system Proprietary file format Proprietary file format SQL database Minimum sampling frequency 1 minute 1 minute 1 second Sample frequency selectable per parameter no no yes Data export to Excel yes yes yes Data export in ASCII format yes yes yes Graph export in BMP format yes yes On line calculations using on line and off line data no yes yes Data display Line graphs yes yes yes Bar graphs yes yes yes Synoptic yes yes yes Procedure flow chart no no yes Combination of current and historic data in graphs yes yes yes Combination of different active batches in graphs yes yes Measured data table display yes yes yes Scatter plots yes yes yes Storage of predefined graph settings yes yes yes Supervisory control Programming of time based actions no yes yes Programming of event based actions no yes yes Profiling of setpoints no yes yes Manual setting of local control set points no yes yes Recipe definition no yes yes Other Notebook for process and equipment remarks yes yes yes Event viewer for system comments yes yes yes Audit trail no no yes Batch reports supported no no yes Remote access no no yes 8 8 Applikon Biotechnology Cultivation Systems from Discovery to Production DOs Sensors Measurements of dissolved oxygen DO2 in biotechnological processes is a basis fo
47. toclavable bioreactors and fermentors are available in 1 2 3 5 7 15 and 20 liter total volume Features e Wide range of volumes with interchangeable modules e Wide range of modules to tailor the systems to the research demands e No welded parts in the bioreactor topplate e Simple set up and easy to handle e Optional high torque magnetically coupled agitator e All metal parts are constructed of stainless steel 316L e External mirror polished finish e Electropolished finish of product contact stainless steel parts ease cleanability e Glass dished bottom vessels are made of borosilicate glass to guarantee resistance to thermal shock excellent corrosion resistance smooth non porous surface for easy cleaning optimal transparency for visual inspection of the culture e Glass bioreactor vessels can be used up to 0 5 barg 7 5 psig of overpressure Applications e Microbial cultures e Cell cultures e Batch e Fed Batch e Perfusion e Continuous cultivation 1 4 Applikon Biotechnology Cultivation Systems from Discovery to Production Specifications Applikon Biotechnology Cultivation Systems from Discovery to Production 1 5 Total Working Minimum Aspect Aspect volume volume working ratio total ratio working L L volume L volume L volume L 1 liter 1 25 0 9 0 3 2 1 1 5 2 liter single wall 2 2 1 7 0
48. tors in the range of 25 to 2 500 liter total volume and ER ATMI iCellis Nano bioreactors for cultivating anchorage dependable cells All systems are unique in their accurate measurement and control of key process parameters The systems can be controlled using the Applikon ez Control i Control or my Control Most systems can be equipped with re usable sensors as well as fluorophor pH and Dissolved Oxygen sensors All systems are supplied tumkey and fully documented All systems go through standard IQ OQ and FAT procedures before they are delivered and installed at the customers site The qualified Applikon technical support team can take care of the installation and will be your first point of contact for any questions Our delivery includes the bioreactor the controller sensors actuators temperature control system supervisory software and validation documentation Our well trained sales consultants are able to advise you on the complete configuration of your single use bioreactor system AppliFlex The AppliFlex bioreactor range consists of a 10 liter 20 liter and 50 liter bioreactor bag The control system pH Dissolved Oxygen temperature mixing is the standard Applikon ez Control The rocking motion offers gentle mixing The same rocker can be used for the 10 2x 20 and 50 Liter bioreactor bags The AppliFlex bioreactors are an easy scale up to the HyClone bioreactors the bags are made of the same materials which eases val
49. truments de Ireland Asistec RTD Technology Ltd Phone 353 1 281 4650 281 4064 www asistec ie Italy Steroglass S r l Phone 39 075 609 091 www steroglass it Spain amp Portugal Fisher Scientific Phone 34 933 069 800 www es fishersci com Sweden AB Ninolab Phone 46 8 590 860 70 www ninolab se Switzerland ReseaChern GmbH Phone 41 344 240 310 www reseachem ch United Kingdom Applikon Biotechnology Phone 44 845 337 3306 www applikon biotechnology co uk Russia Abacus Analytical System Phone 7 495 739 0363 www abacus lab com Poland Witko Sp z 0 0 Phone 48 426 763 435 www witko com pl Turkey Spektra Lab Laboratuvar Cihazlari Ltd Phone 90 312 447 7709 www spektralab com Australia amp New Zeeland Australia John Morris Scientific Phone 61 2 9496 4200 www_johnmorris com au Asia y Singapore x Zil Sci Pte Ltd Phone 65 676 729 29 www zilsci com India BioGentek BG PW Ltd Phone 91 11 562 2255 551 3104 www biogentek com Indonesia Pt Arico Sainsindo Nusantara Phone 62 21 580 6342 3 www ptarico com China Applitech Pharma Phone 86 203 482 1111 ext 8216 www applitechpharma com Applikon Biotechnology China Yuexiu District Guangzhou P R China Korea Seoulin Bioscience Co Ltd Phone 82 1670 5911 www seoulin co kr Taiwan Tseng Hsiang Life Science Ltd Phone 86 2 2785 1156 www thco com tw Middle
50. version BioXpert W7 focuses on process repeatability and data security ee d ze BP piion Mi deere Features BioXpert seamlessly integrates with Applikon and other makes of controllers and peripheral equipment to enable you to get the maximum out of your cultivation system Pre defined recipes exist for microbial culture mammalian cell culture and plant cell culture All process data and controller data can be stored at user defined intervals Off line measurements can be added to the process data to be used for calculations or advanced process control BioXpert offers different ways of presenting your measured data trend graph table and bar graph The on line calculations of BioXpert can be used to calculate derived variables The statement editor and interpreter allow time and or event based execution of advanced control strategies Another option for supervisory control is the use of timed profiles Remote Access for BioXpert W7 makes process data accessible for monitoring throughout a network using web browser technology Applications e BioXpert software can be used for Microbial and Cell cultures in Batch Fed Batch Perfusion and Continuous cultivation Specifications 36 Applikon Biotechnology Cultivation Systems from Discovery to Production Specifications BioXpert Lite BioXpert 2 BioXpert W7 General Operating system Windows NT Windows NT Windows XP service pack 3 2000 XP Vi
51. ystem e Accurate process control e Qualified support and service e Classic and single use fluorophor sensors for e Cell culture measurement of pH Dissolved Oxygen e Batch e Accurate control of pH DO temperature mixing e Fed Batch e Data logging amp SCADA 21CFR part 11 available e Perfusion K e Turn key delivery e Suspension cells e Q OQ FAT and SAT for the complete system e Anchorage dependent cells 2 2 Applikon Biotechnology Cultivation Systems from Discovery to Production BioBundle complete cultivation systems A BioBundle is a complete bioreactor system eq silicone tubing sample bottles and a starter kit i uipped with all necessary components and is ready to use out of the box No detail is overlooked the system is complete with ncluding spare parts The BioBundle is easy to set up requires no special skills or tools easy to learn and easy to operate BioXpert Lite Software for data acquisition is included Select one or more of the optional add on packs to customize your BioBundle The BioBundle provides a unique Ethernet computer interface allows easy integration of the ez Controller into your network USB connection TFT color touch screen interface for easy navigation and clear data display High precision motor pH dissolved oxygen and temperature sensors Gas mixing and easy gas selection system Easy access headplate Up
52. zation time 30 minutes Minimum polarization time after autoclaving 4h Optimum polarization time after autoclaving Overnight Polarization current Air 25 C 1 013 bar 100 RH 83 66 nA Polarization current Air 40 C 1 013 bar 100 RH 65 98 nA Polarization current O2 40 C 1 013 bar 0 RH 300 350 nA Response time gas phase 20 C t 90 20 30 s Response time gas phase 40 C t 90 10 20 s Response time gas phase 60 C t 90 5 10 s Drift between 15h 5 days lt 0 2 day Drift between 5 days and 7 days lt 0 1 day Drift between 7 days and 30 days lt 0 05 day Insertion length o 12 mm sensors 1 4 Gi ZS E Weg La ege 0 Z010011025 insertion length 110 mm 215 mm total length used with Tracfix sensor holder Z010015420 insertion length 154 mm 259 mm total length Z010023525 insertion length 235 mm 340 mm total length Bioreactor 1 5 L Z010032525 insertion length 325 mm 430 mm total length Bioreactor 7 L Z010042525 insertion length 425 mm 530 mm total length Bioreactor 15 L Z010059025 insertion length 590 mm 695 mm total length Bioreactor 20 L Insertion length o 8 mm sensors Z010015080 insertion length 150 mm Z010017080 insertion length 170 mm AO Applikon Biotechnology Cultivation Systems from Discovery to Production oH sensors In the biotech and pharmac

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