ÄKTAmicro™ - GE Healthcare Life Sciences
Contents
1. 16 6 11 Start up the AKTAmicro separation unit 16 6 1 2 Start up the computer and the UNICORN software 16 6 2 Preparation of PUMP P 905 17 6 2 1 Priming and purging Pump P 905 ue 17 6 2 2 Setting compression 18 6 2 3 Testing pressure stability 19 6 3 Running the installation test method 20 6 4 Evaluating the installation test results 23 6 4 1 Automatic evaluation 23 6 42 Man dl evaluation transmet 23 6 5 Correcting faulty evaluation results 25 6 5 1 Faulty gradient 6 5 2 Faulty step response 7 Testrecord stable dele a ila 26 7 1 Gradient test result reo eceeeeionieneo 26 7 2 Step response test result ccccssssssscsssssesssesssssseseseseecsesees 26 7 3 UV response test result csssssssssssssesssssssesesssssssssscessesesesees 26 8 Installation record ceseeeaes 27 9 Registration FOrM cscssscscssececcecnsessssscssesssssssses 29 10 30 AKTAmicro Installation Guide 28 9490 95 AB 5 AKTAmicro Installation Guide 28 9490 95 Edition AB About this installation guide 1 1 About this installation guide AKTAmicro is assembled and fully tested befor2. 90 Step response test result 1 2 3 4 5 Programmed Value read Baseline Normalized Allowed Conc B corrected value interval value 100 95 94 96 70 69 71 30 29 31 5 4 6 0 7 3 UV response test result UV response table 1 2 3 amp 5 Wavelength Value read Baseline cor Absorbance Allowed nm 100 B 0 B rected value ratio interval 254 265 254 1 11 1 26 265 265 280 1 26 1 53 280 AKTAmicro Installation Guide 28 9490 95 Edition AB 8 Installation record Installation record 8 Check 1 Unpacking Contents according to packing lists All packing material removed No visible damage Sign Remarks 2 Installation Injection valve waste tubings port 4 and 5 marked W1 and W2 to waste reservoir Column holder installed Computer and printer installed UniNet 1 cabling installed Mains power cabling installed Installation test Solutions prepared Tubings to piston seal rinsing system in 20 ethanol AKTAmicro prepared Installation Test method run Installation Test results evaluated Test Record completed Registration Form completed Test Record and copy of Registration form stored in System Logbook Registration form posted to Service Administration nstallation Guide stored in User Manual box for future use AKTAmicro Installation Guide 28 9490 95 Edition AB 27 8 Install
3. cover locate in holes at the front and rear of the cell holder The cover is then lowered over the cell holder Note You can select a fraction collector an autosampler or an MS unit as optional components Installing the fraction collector and the autosampler is described in the AKTAmicro Optional Configurations User Manual The MS must be installed by authorized service personnel AKTAmicro Installation Guide 28 9490 95 Edition AB 13 5 Installation of AKTAmicro 5 2 Installing the computer and the UniNet cables 1 Unpack and install the computer and printer according to the manufacturer s instructions Place them to the left of the system Do not switch them on CAUTION The mains power to AKTAmicro must be switched OFF before the UniNet 1 cabling is installed 2 Complete the UniNet 1 data communication chain by connecting a UniNet 1 cable between the computer and Pump P 905 CAUTION The UniNet connection to the computer MUST be made to the board with four LEDs 37 3 Ifusing a fraction collector connect al il the UniNet 1 cable 35 between Pump P 905 and the fraction collector and UniNet 1 cable 36 Fraction between the fraction collector and collector the computer optional 4 Ifusing an autosampler the UniNet a 1 cable is routed via one of the UniNet 1 con
4. the intervals given in column 5 24 AKTAmicro Installation Guide 28 9490 95 Edition AB Installation test 6 Evaluating the UV response Calculate the UV response ratios in the following way 1 Subtract the base line values 0 B corresponding to each UV curve from the values in column 2 of the UV response table of the Test record see page 26 and enter the results in column 3 2 Calculate the absorbance ratios 265 nm 254 nm and 265 nm 280 nm using the values of column 3 and enter the results in column 4 The ratios obtained should all fall within the intervals given in column 5 6 5 Correcting faulty evaluation results Should any of the evaluated values fall outside the specified range proceed as follows If the system differs from the standard configuration evaluate the result manually If the faulty evaluation result remains continue below 6 5 1 Faulty gradient e The mixer is faulty e Disturbances may arise from air in the pump pump valves or bad sealings in the pump Refer to the Pump P 905 User Manual 6 5 2 Faulty step response e Ifall values are faulty air in the pump or a faulty pump e 5 and 95 faulty bad sealing in the pumps 5 faulty pump module B 95 faulty pump module A AKTAmicro Installation Guide 28 9490 95 Edition AB 25 7 Test record 7 26 Test record Date ins 7 1 Gradient linear from 7 2 Step response table Gradient test result B 10
5. the rinsing tubing in a flask 1 containing 20 ethanol in distilled water 2 Connect a syringe to the rinsing tubing that is connected to the underside 2 of the left pump head on pump A Slowly draw rinsing solution to the syringe When rinsing solution starts to enter the syringe continue to draw a few milliliters 3 Loosen the syringe and immerse the tubing in the rinsing solution 1 Purge Pump P 905 as follows To turn the purge valve a 10 mm spanner is required The torque used to tighten the valve is 1 2 to 1 8 Nm 1 Immerse the inlet tubing of all pump modules with filters in a distilled water flask Note Never place the flask below the level of the pump inlet 2 Connect a male Luer syringe of about 30 ml to the open end of the purge tubing 3 Connect a male Luer connector at the other end of the purge tubing to the left purge valve at pump module A 4 Turn the purge valve counter clockwise half a turn to open it and slowly draw eluent into the syringe AKTAmicro Installation Guide 28 9490 95 Edition AB 17 6 Installation test Setup Compr comp off off on Setup Start comp off off on 18 5 When fluid starts to enter Purge valve the syringe continue to draw f a few milliliters before closing the purge valve Check that there is no visible air left in the inlet tubing 6 Repeat steps 3 to 5 for the other pump heads 6 2 2 Setting compre
6. Amicro The installation test can also be used at any time to check the condition of the system e g for calibrating the system regularly or after a prolonged stop Correct gradient formation is tested by producing a linear gradient and a series of concentration steps of acetone Correct UV monitoring is tested by monitoring the acetone concentration at 265 254 and 280 nm and calculating the absorbance ratios 265 254 nm and 265 280 nm 6 1 Start up the AKTAmicro system 6 11 Start up the AKTAmicro separation unit 1 Switch on the separation unit using the ON OFF switch located to the left on the base platform 6 12 Startup the computer and the UNICORN software 1 Switch on the computer the display and the printer according to the instructions in the manufacturer manuals Log into Windows by first pressing Ctrl Alt Del and then clicking on OK Sune RN S A 3 When the Windows desktop appears start UNICORN by double clicking on the UNICORN icon 4 Select user default and enter default as password Click on OK CT x ae UNICORN logon User name default Password et Cancel Help 16 AKTAmicro Installation Guide 28 9490 95 Edition AB Installation test 6 5 Click on the System Control button in the task bar System Control Button 47 UNICORN Manager Se Method Editor UNTIT lt DEMO System Contro amp Evaluation 6 2 Preparation of Pump 905 6 2 1 Priming and purging Pump P 905 1 Immerse
7. END 6 2 3 Testing pressure stability Perform a pressure test to establish that all air has disappeared from the pump heads Perform as follows Connect a capillary between port 1 on the Injection valve to the inlet of the UV optical unit Run 0 2 ml min at 0 B distilled water Check on the pump display that the pressure reading is stable variation lt 5 Run 0 2 ml min at 100 B distilled water Check on the pump display that the pressure reading is stable variation lt 5 Proceed to step 5 if the pressure is stable If not consult the Pump P 905 User Manual for trouble shooting instructions Click on END Transfer the inlet tubing 1 into a flask containing 100 ml of 0 4 acetone in distilled water Installation Test Method Guide Buffer A Distilled water Buffer B 0 4 acetone in distilled water Test flow rate 1 ml min Test run time Approximately 30 minutes AKTAmicro Installation Guide 28 9490 95 Edition AB 19 6 Installation test 6 3 Running the installation test method 1 In UNICORN Manager module select File Printer Setup Select the appropriate printer from the list and select Landscape Then click OK to acknowledge the printer chosen Print Setup 2 In System Control module select File Instant Run The Instant Run window opens InstantRun 3 Select the appropriate system from the For system list 4 Click Run The Method Wizard is started 5 Select Instal
8. GE Healthcare AKTAmicro Installation Guide KTA AKTAmicro Installation Guide 28 9490 95 Edition AB Important user information All users must read this entire manual to fully understand the safe use of AKTAmicro Important AKTAmicro is intended for research use only and should not be used in any clinical or in vitro procedures for diagnostic purposes Safety notices This manual contains warnings and cautions concerning the safe use of the product See definitions below WARNING The WARNING symbol and notice highlight instructions that must be followed to avoid personal injury Do not proceed until all stated conditions are clearly understood and met CAUTION The CAUTION notice highlights instructions that must be followed to avoid damage to the product or other equipment Do not proceed until all stated conditions are met and clearly understood Notes Note A Note is used to indicate information that is important for trouble free and optimal use of the product Recycling This symbol indicates that the waste of electrical and electronic equipment must not be disposed as unsorted municipal waste and must be collected separately Please contact an authorized representative of the manufacturer for information concerning the decommissioning of equipment I WARNING This is a Class A product In a domestic environment it might cause radio interference in which case the use
9. ample loop connected to ports 2 and 6 of the injection valve This prevents liquid spraying out of the ports when switching the valve which is especially dangerous if hazardous chemicals are being used WARNING Two people are required to lift the system WARNING Never block the flowpath outlet since this will create over pressure and may result in injury WARNING Only spare parts approved or supplied by GE Healthcare may be used for maintaining and servicing the system WARNING Never place waste containers on the top of the system If they become full and overflow liquid may penetrate the system causing a short circuit AKTAmicro Installation Guide 28 9490 95 Edition AB Pre requisites 3 3 Pre requisites A WARNING AKTAmicro must be connected to a grounded mains socket e Two people are recommended to lift AKTAmicro onto the working bench e To install AKTAmicro a working area of about 200 x 80 cm is required e AKTAmicro requires 100 to120 220 to 240 VAC 50 60 Hz electrical supply with safety grounding e Cutting pliers are recommended for cutting plastic straps e Awaste flask IMPORTANT Before using AKTAmicro equipment read all the safety information in section 1 2 in AKTAmicro System Manual The installation test requires the following solutions e 200 ml of distilled water for priming and purging the pump e 100 ml of 0 4 acetone in distilled water
10. ation record 28 AKTAmicro Installation Guide 28 9490 95 Edition AB 9 Registration form Registration form 9 IMPORTANT WARRANTY REGISTRATION INFORMATION to register the users equipment under warranty Please ensure that this form is completed and returned to Service Administration NAME IniStitute COM PONY Redi DepartmMent lOCAtions s s iscccisciseisscccsssczsceceedentssosessassetssecscetsscdoursnscseatencdbedutasatess Post Code cc LO Rita Phone Number ns Fax Number rr Date of Installation rrrrrrerereeeee Quote No Customer Order nn INVOICE Note Fill in serial numbers over leaf AKTAmicro Installation Guide 28 9490 95 Edition AB Support Agreement purchased with the instrument IVES give details ses GA Install r names Y N 29 10 Components 10 Components QTY AKTAmicro serial Part Number Description System rack Serial Number Mixer M 925 Monitor UV 900 Pump P 905 Monitor pH C 900 INV 917 SV 903 A SV 903 B Computer Computer display 30 AKTAmicro Installation Guide 28 9490 95 Edition AB For local office contact information vi
11. e 100ml of 20 ethanol in distilled water AKTAmicro Installation Guide 28 9490 95 Edition AB 9 4 Installation overview 4 Installation overview e Unpack AKTAmicro page 11 e Detach secured items and install items enclosed page 12 e Unpack and install the computer page 14 e Connect mains power cabling page 14 e Connect UniNet 1 data communication chain cabling page 14 e Complete the first two sections of the installation record page 27 e Prepare AKTAmicro for the installation test page 16 e Run the installation test method page 20 e Evaluate the gradient page 24 e Evaluate the step response page 24 e Evaluate the UV response page 25 e Complete the test record page 26 e Complete the registration form page 29 e Complete the final section of the installation record page 27 e Store photocopies of all records and forms in the System Logbook e Store the Installation Guide in the User Manual box 10 AKTAmicro Installation Guide 28 9490 95 Edition AB Installation of AKTAmicro 5 5 Installation of AKTAmicro Begin by creating a clean and dry working area of 200 x 80 cm that allows easy access Then follow the step by step instructions below and fillin the installation record as you go along see page page 27 Note Some components are packed in Box 900 located at the top of the system Note Some packing lists are included in the paper boxes 5 1 Unpacking 1 After having removed the cardboard hood check the content
12. e shipping For safe transportation however some components have been secured and thus need to be detached before the system can be tested and used Cables capillaries accessories etc are enclosed in paper boxes or in Box 900 located at the top of the instrument pile This guide describes how to install AKTAmicro The guide is divided into two parts one describing the installation and one describing how to run the installation test After the installation procedure has been performed your AKTAmicro is ready for purification work For full details of specifications methods maintenance etc refer to the respective User Manuals and Instructions AKTAmicro Installation Guide 28 9490 95 Edition AB 7 2 Safety 2 Safety gt PPR RRPRR PE e The system is designed for indoor use only e Donot use ina dusty atmosphere or close to spraying water Refer to Technical Specifications in the System Manual for detailed environmental pre requisites WARNING The AKTAmicro system operates under high pressure Eye protection must be worn at all times WARNING The individual modules must not be opened by the user They contain high voltage circuits that can give a lethal electric shock WARNING Monitor UV 900 uses high intensity ultra violet light Do not disconnect the optical unit while the lamp is ON WARNING AKTAmicro must be connected to a grounded mains socket WARNING There must always be a s
13. g to Installation Test01 1_UV1_265nm 01 SMTH Right click in the Curves window and click on the Marker Move the vertical marker to the constant section of each plateau by dragging it Enter the absorbance values in MAU in column 2 in the Step response table of the Test record see page 26 leaving out the decimals 8 Read the absorbance for the plateaus corresponding to 0 B and 100 B for the curves click on the curve name to change the curve reading e Installation Test01 1_UV1_265nm 01 SMTH e Installation Test01 1_UV2_254nm 02 SMTH e Installation Test01 1_UV3_280nm 03 SMTH Enter the values in column 2 in the UV response table of the Test record see page 26 9 Click on Print under File to print out the chromatogram Evaluating the gradient Place a ruler along the gradient part of curve Installation Test01 1_UV1_265nm 01 SMTH in the printed report The curve should be linear between 10 B and 90 B and void of discontinuities Evaluating the step response Calculate the relative adsorption plateau heights for curve Installation Test01 1_UV1_265nm 01 SMTH as follows 1 Subtract the base line value 0 B from each of the values in column 2 in the Step response table of the Test record see page 26 and enter the results in column 3 2 Divide each value in column 3 by the base line corrected value corresponding to 100 B multiply by 100 and enter the results in column 4 The values of column 4 should all fall within
14. lation_Test from the Main Selection list and click Run Method Wizard for System System 1 20 AKTAmicro Installation Guide 28 9490 95 Edition AB Installation test 6 6 Click Next in the Evaluation Procedures window 7 Click Next in the Method Information window 8 Make sure that the compression compensation value for water 4 6 x 10 19 Part is set in Pump 905 refer to section 6 2 2 Setting compression compensation 9 Click START in the Result Name window to start the installation test The progress of the test is monitored in the System Control module The installation test run time is approximately 30 min When the test is finished the printer automatically prints the chromatogram and the test results Installation Test01 1 result Step response result AKTAmicro Installation Guide 28 9490 95 Edition AB 21 6 22 Installation test Customizing the Curves pane To customize the Curves pane 1 2 3 Right click in the Curves pane and select Properties Click the Curves tab Properties Select the following curves to be displayed e UV e Conc Clear all other curves Click OK AKTAmicro Installation Guide 28 9490 95 Edition AB Installation test 6 6 4 Evaluating the installation test results 6 4 1 Automatic evaluation The system automatically prints out the test result when the test is finished The print out consists of a chromatogram and an eval
15. move foam Cut here e Plastic hood covering the Injection Valve and the Mixer including the PEEK tubings e Injection Valve INV 917 located at the top left side Detach the valve from the attachment bracket to remove the protective foam and reposition the valve to the slot in the attachment bracket e Mixer M 925 located next to INV 917 Detach the Mixer from the attachment bracket to remove the protective foam and reposition the mixer to the same slot in the attachment bracket e SV 903A and SV 903B located between the pump heads on P 905 AKTAmicro Installation Guide 28 9490 95 Edition AB 10 Installation of AKTAmicro 5 e UV cell cover located above the UV flow cell Remove the red tapes e Capillary loops Remove the red tape holding the capillary loops attached to INV 917 waste capillaries W1 and W2 Place the waste tubings W1 and W2 in waste bottles and place the bottles in front of AKTAmicro or wherever convenient Unpack the inlet tubing fixed in a plastic bag taped to the buffer tray on top of Box 900 Make sure all items are securely fitted and that no capillary connection has worked loose or been tangled Attach the column holder enclosed in Box 900 above the UV cell Select a slot to suite the height of the column to be used Should the cover over the UV cell compartment come loose it is refitted in the following way The cover is a simple push fit onto the cell holder Two small lugs on the
16. nectors in the autosampler before connecting to the computer All other UniNet 1 cables are connected at delivery An MS unit used with the AKTAmicro system communicates through serial standard cables with the computer See the documentation of the MS unit to connect the unit 14 AKTAmicro Installation Guide 28 9490 95 Edition AB Installation of AKTAmicro 5 5 3 Connecting the mains cables A WARNING AKTAmicro must be connected to a grounded mains socket 5 Connect a mains cable 39 supplied between AKTAmicro and a properly grounded mains socket Do not switch on Fraction collector optional 6 If using a fraction collector connect a mains cable supplied 38 between the fraction collector and a mains socket at the rear of AKTAmicro 7 If using an autosampler connect a AR mains cable between the z autosampler and a mains socket at the rear of AKTAmicro To fraction auto To mains socket 8 Complete the two first sections of collector sampler the Installation record on page page 27 The installation phase of AKTAmicro is now completed AKTAmicro Installation Guide 28 9490 95 Edition AB 15 6 Installation test 6 Installation test The installation test checks the function of the solvent delivery and the UV monitoring system of AKT
17. r might be required to take appropriate measures personnel authorized by GE Healthcare Do not open any covers or replace parts unless specifically stated in the instructions WARNING All repairs should be done by CE certification This product complies with the European directives listed below by fulfilling corresponding standards A copy of the Declaration of Conformity is available on request e 73 23 EEC Low Voltage Directive e 89 336 EEC EMC Directive The CE logo and corresponding declaration of conformity is valid for the instrument when it is used as a stand alone unit or connected to other CE marked GE Healthcare instruments or connected to other products recommended or described in this manual and used in the same state as it was delivered from GE Healthcare except for alterations described in this manual Note The Declaration of conformity is valid only for systems that are marked with the CE logo CE Contents 1 About this installation guide tt rr 7 2 Sofia nr 8 dents 9 Installation overview severeseo 10 5 Installation of KTAM ICTO cenere 11 BL niet 11 5 2 Installing the computer and the UniNet cables 14 5 3 Connecting the mains 65 15 6 Installation test uns ns Auts 2 16 6 1 Start up the KTAmicro system
18. s against the attached packing list Check also all included boxes Store all the enclosed paper boxes and plastic bags in a convenient nearby place 2 Release and remove the red strap 1 holding the system to the pallet 3 Lift AKTAmicro onto the work area using the four strap handles 2 Two people are required to lift the system Do not raise to upright position yet Lay the system on the same side as on the pallet 4 Release the two red straps with the strap handles Allow the straps to remain taped to the plastic cover 5 Pull back the plastic cover 3 to uncover the swivel platform 4 and raise the system to an upright position 6 Remove the plastic cover with the red straps from the system AKTAmicro Installation Guide 28 9490 95 Edition AB 11 5 Installation of AKTAmicro 12 Save all the original packing material If for any reason the equipment has to be repacked for transportation or otherwise it is important that the system can be safely packed using the original packing material Turn AKTAmicro on its swivel platform to access the fluid component side of the system Detach the following items by cutting straps and removing red tape Also remove the desiccant bags Note Use cutting pliers Be careful not to cut any capillaries by accident Make sure not to lose any of the capillary marking tags Cut here and re
19. sit www gelifesciences com contact GE Healthcare Bio Sciences AB Bj rkgatan 30 751 84 Uppsala Sweden www gelifesciences com AKTA imagination at work GE imagination at work and GE monogram are trademarks of General Electric Company AKTA AKTAmicro DropDesign and UNICORN are trademarks of GE Healthcare companies UNICORN Any use of this software is subject to GE Healthcare Standard Software End User License Agreement for Life Sciences Software Products A copy of this Standard Software End User License Agreement is available on request All third party trademarks are the property of their respective owners 2009 General Electric Company All rights reserved First published Feb 2009 AII goods and services are sold subject to the terms and conditions of sale of the company within GE Healthcare which supplies them A copy of these terms and conditions is available on request Contact your local GE Healthcare representative for the most current information GE Healthcare Europe GmbH Munzinger Strasse 5 D 79111 Freiburg Germany GE Healthcare UK Ltd Amersham Place Little Chalfont Buckinghamshire HP7 9NA UK GE Healthcare Bio Sciences Corp 800 Centennial Avenue P O Box 1327 Piscataway NJ 08855 1327 USA GE Healthcare Bio Sciences KK Sanken Bldg 3 25 1 Hyakunincho Shinjuku ku Tokyo 169 0073 Japan 28 9490 95 AB 03 2009
20. ssion compensation When running Pump P 905 at high pressures flow losses might occur due to the compression of the eluent Pump P 905 has functions that compensate for these flow losses Note The compensation functions should be activated at all times With the compensation function activated the pump takes into consideration the given compressibility for the liquid at the actual pressure Some common values are given below Liquid Value in Pa 1 Water 4 6 x 10 10 Methanol 12 1 x 10 10 Most other organic solvents e g ethanol 11 0 x 10 10 Before starting the installation test make sure that the compensation value is set to 4 6 x 10 10 Par for water To set the compression compensation 1 Turn the front selection Dial on the pump 2 Select main menu Setup press OK 3 Select sub menu Setup Compr comp press OK 4 Change numbers accordingly and select On to activate the compensation Press OK 5 Select sub menu Setup Start comp press OK 6 Select On to activate the start compensation Press OK AKTAmicro Installation Guide 28 9490 95 Edition AB Setup Compr value A 4 6E 10 4 6 Setup Compr value B 4 6 10 4 6 10 11 Installation test 6 Select sub menu Setup Compr value A press OK Enter compensation value 4 6 x 10 10 Pa for pump module A Select sub menu Setup Compr value B press OK Enter compensation value 4 6 x 10 10 Pat for pump module B Press ESC ESC gt
21. uation of the test result e Ifthe gradient test result is OK the print out says Gradient linearity accepted e Ifthe step response test result is OK the print out says Step response accepted e Ifthe UV response test result is OK the print out says UV response accepted If any of the evaluated values falls outside the specified range go to section 6 5 Correcting faulty evaluation results 6 4 2 Manual evaluation If your chromatography system differs from the standard configuration for example if optional components have been installed the automatic evaluation may not give a reliable result A manual evaluation is required 1 Minimize the run data window to access the Main menu 2 Clickon in the results window and then double click on the Installation Test01 icon to open the result file 3 aximize the chromatogram window by clicking on El in the upper right corner D Q ick in the Curves window with the right mouse button and select Properties 5 Click on the Curves tab and select the following curves to displayed e Installation Test01 1_UV1_265nm 01 SMTH e Installation Test01 1_UV2_254nm 02 SMTH e Installation Test01 1_UV3_280nm 03 SMTH e Installation Test01 1_ Conc 6 Press OK AKTAmicro Installation Guide 28 9490 95 Edition AB 23 6 Installation test 7 Click on the UV curve name in the upper left corner of the chromatogram window and read the absorbance for the steps correspondin
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