ISO-01B Manual - NPI Electronic Instruments
Contents
1. Electronic Instruments for the Life Sciences pease jade 1C OPERATING INSTRUCTIONS AND SYSTEM DESCRIPTION FOR THE ISO STIM 01B STIMULUS ISOLATOR ISO STIM 01B STIMULUS ISOLATOR npi U OPERATION MODE AMPLITUDE UNIPOLAR SE O Q lt 9 lt 7 s amp v OFFSET CAP COMP 50 ETME OUTPUT us FULL SCALE TRIGGER INPUT DIRECT INPUT ISOLATED OUTPU HASSIS TTL TTL CAUTION HIGH VOLTAGE p a M Ra x ou M VERSION 1 2 npi 2014 npi electronic GmbH Bauhofring 16 D 71732 Tamm Germany Phone 49 0 7141 9730230 Fax 49 0 7141 9730240 support npielectronic com http www npielectronic com ISO STIM 01B User Manual Table of Contents hs ALLY COMIAUIONS essi a Gules lean sreked E 3 2 Special Safety Notice for High Voltage Instruments sees eee eee 4 w SOS HOR Baa a treet ne ret a E rene rrr 5 Sale System DESCrpPllO i TTT 5 32s R e Kele OF Che Font Pane ies asceste sn sa cemnsuemaaless e a 5 33 Descniphonm orhe Teal pahe lren a a a ean 8 ers E UU MO TTT 9 3 4 1 Connections Noise Reductio nkee seenen a A eee 9 3 4 2 Operation Mode Seana a ns eae Se bas aateee aim neaeaaeennaie 9 4 Battery Pack andG Wak Ger T 10 Da POWT SUP DIY TET 11 6 Technical Data ISO STIM 0 Brci E 12 version 1 2 page 2 ISO STIM 01B User Manual 1 Safety Regulations VERY IMPORTANT Instruments and components supplied by npi electronic are NOT intended for clinical use or medical pu2. FF respectively 3 DURATION ms digital potentiometer Potentiometer that sets the duration of the stimulus generated by the built in timing unit version 1 2 page 5 ISO STIM 01B User Manual 4 OPERATION MODE BIPOLAR UNIPOLAR switch 5 AMPLITUDE 10 turn potentiometer Potentiometer that sets the amplitude of the stimulus generated by the internal timing unit The maximum range is selected with the OUTPUT rotary switch 15 6 POLARITY switch Switch for setting the polarity of the stimulus 7 OVER LED LED indicating that the amplifier is 10 below its positive limit limit is 100V in VOLTAGE OUTPUT MODE In CURRENT OUTPUT MODE the limit 100 V is set by the corresponding voltage at the electrode due to Ohm s Law V I R 1 e this control indicates during an experiment in CURRENT OUTPUT MODE that the electrode resistance increases and the amplifier is not be able to provide the current which is set if the electrode resistance increases further 8 STIM LED LED indicating the application of a voltage or current stimulus 9 OVER LED See 7 10 CAP COMP trim pot The CAP COMP potentiometer compensates for the input capacitances in isolated CURRENT output operation only Capacity compensation is achieved by turning the potentiometer with a small screw driver clockwise until the current signal at the oscilloscope is as square as possible Important If the CAP COMP is overcompensated in C
3. O SO 01B FUSE SLOW 1 25A gt L GC POWER ON CAUTION k FOR USE WITH ISO 018B ONLY Figure 6 Front panel left and rear panel right view of the ISO STIM 01B power supply The power supply is connected to mains with a standard power cord It has a POWER ON switch on the rear panel Important Only use the supplied power supply to power the ISO STIM O1B Using different power supplies might result in damage of ISO STIM 01B and or power supply Important This power supply is designed for use with the ISO STIM OIB only Using it differently might result in damage of the power supply version 1 2 page 11 ISO STIM 01B User Manual 6 Technical Data ISO STIM 01B Input resistance Output Output voltage Output current IPG interphase gap Charging current Charging time Capacity battery pack Power requirements Fuses Dimensions version 1 2 2kQ monopolar or bipolar selected by toggle switch 100 V max isolated 10 mA max isolated 50 500 us 25 mA cell normal 50 mA cell fast from empty to fully charged 12 h normal 6 h fast 500 mAh low voltage part 250 mAh high voltage part 100 240 V AC only use supplied charger battery pack or power supply Battery Charger 1 25 A slow Power Supply 1 25 A slow ISO STIM 01B 246 x 188 x 89 mm W x D x H Battery Pack 246 x 188 x 89 mm W x Dx H Battery Charger 103 x 169 x 59 mm W x D x H Pow
4. URRENT MODE the stimulus isolator will ring and not work properly 11 CHASSIS connector Jack linked to the CHASSIS The green banana connector of the supplied cable is connected here 12 ISOLATED OUTPUT plug negative The ISOLATED OUTPUT signal is available at two plugs red and black The polarity of the POLARITY switch 6 is not changed 1 e the red plug is positive if the output polarity switch is set to This signal is completely isolated from ground The black jack serves as reference point for the isolated output signal The black banana connector of the supplied cable is connected to the black jack and the red to the red jack Caution THIS INSTRUMENT HAS A HIGH VOLTAGE OUTPUT UP TO 100 V Do not touch these pins or bare wires connected to these pins Always turn power off if you manipulate devices connected to these pins 13 ISOLATED OUTPUT plug positive See 12 version 1 2 page 6 ISO STIM 01B User Manual 144 OFFSET trim pot Trim pot to compensate for the OFFSET of the stimulating electrode It 19 recommended to compensate the offsets only in a completely warmed up condition 1 e after 30 minutes warm up time Compensation procedure is also possible in current mode 15 OUTPUT rotary switch Switch for selecting current or voltage stimulation and for setting the maximum range to be set with the AMPLITUDE potentiometer 5 16 IPG TIME us potentiometer with switch Potentiomete
5. charge and must therefore be handled with care Electrostatic discharge can be avoided by touching a grounded metal surface when changing or adjusting sensors Always turn power off when adding or removing modules connecting or disconnecting sensors headstages or other components from the instrument or 19 cabinet TEMPERATURE DRIFT WARM UP TIME All analog electronic systems are sensitive to temperature changes Therefore all electronic instruments containing analog circuits should be used only in a warmed up condition 1 6 after internal temperature has reached steady state values In most cases a warm up period of 20 30 minutes is sufficient HANDLING Please protect the device from moisture heat radiation and corrosive chemicals version 1 2 page 3 ISO STIM 01B User Manual 2 Special Safety Notice for High Voltage Instruments 1 2 3 4 5 6 HIGH Y OLT AGE RISK OF ELECTROCUTION Observe extreme caution when working with this instrument Always connect high voltage power supplies to protective earth Do not touch connections unless the instrument is turned off and the capacitance of both the load and power supply are earthed Allow adequate time for discharge of internal capacitance of the power supply Do not ground yourself or work under wet or damp conditions Servicing should be only done by qualified personnel aware of the hazards If in doubt return to supplier for servicing
6. er Supply 103 x 169 x 59 mm W x D x H page 12
7. r to select the interphase gap time IPG in bipolar stimulation mode see 3 4 2 IPG is turned off in the leftmost position switch 17 DIRECT INPUT TTL BNC connector A TTL signal can be fed in here to trigger the output of the ISO STIM 01D Amplitude will be set by 5 6 15 In unipolar mode 4 the output will be active as long as a TTL HIGH signal is fed into this input In bipolar mode the output will be active for the time a TTL HIGH signal is fed into this input After this time an additional puls is generated at the output with the same duration and amplitude but with opposite polarity The time between these two pulses is set by the IPG TIME potentiometer 16 18 TRIGGER INPUT TTL BNC connector A TTL signal can be fed in here to trigger the internal timing unit Duration of the output signal is set by the DURATION potentiometer 3 Amplitute is set by 5 6 15 19 MANUAL TRIGGER pushbutton swich This pushbutton has the same functionality as the TTL input 18 but is for manual operation 20 REMOTE TRIGGER BNC connector optional This connector can be used to connect a passive remote hand switch provided by npi version 1 2 page 7 ISO STIM 01B User Manual 3 3 Description of the rear panel Figure 2 rear panel view of ISO STIM 01B On the rear panel of the ISO STIM 01B there is only one SubD connector This connector is used to connect the ISO STIM 01B either to its power supply or to the ba
8. rposes e g for diagnosis or treatment of humans or for any other life supporting system npi electronic disclaims any warranties for such purpose Equipment supplied by npi electronic must be operated only by selected trained and adequately instructed personnel For details please consult the GENERAL TERMS OF DELIVERY AND CONDITIONS OF BUSINESS of npi electronic D 71732 Tamm Germany 1 2 3 4 5 GENERAL This system is designed for use in scientific laboratories and must be operated by trained staff only General safety regulations for operating electrical devices should be followed AC MAINS CONNECTION While working with the npi systems always adhere to the appropriate safety measures for handling electronic devices Before using any device please read manuals and instructions carefully The device is to be operated only at 115 230 Volt 60 50 Hz AC Please check for appropriate line voltage before connecting any system to mains Always use a three wire line cord and a mains power plug with a protection contact connected to ground protective earth Before opening the cabinet unplug the instrument Unplug the instrument when replacing the fuse or changing line voltage Replace fuse only with an appropriate specified type STATIC ELECTRICITY Electronic equipment is sensitive to static discharges Some devices such as sensor inputs are equipped with very sensitive FET amplifiers which can be damaged by electrostatic
9. ttery pack version 1 2 page 8 ISO STIM 01B User Manual 3 4 Operation 3 4 1 Connections Noise Reduction The ISO STIM 01B comes with a battery pack with a SubD cable which is connected to the stimulus isolation device The corresponding connector at the ISO STIM 01B are located at the rear panel Note A possible source of noise is the chassis of the stimulator In a noisy environment it may act as an antenna picking up 50 Hz or 60 Hz noise respectively especially when operating with current stimuli In this case the noise is substantially reduced if the chassis is connected to ground see also 11 Figure 1 The stimulation electrode is connected to the isolated output see Figure 1 We recommend to use a bipolar electrode for stimulating nerve bundles This will produce only a short stimulation artifact at the beginning Remember If unipolar electrodes are used the whole slice will be stimulated resulting in a DC shift of the whole slice 3 4 2 Operation modes BIPOLAR The isolator is capable to generate biphasic stimuli by switching the stimulus mode switch to BIPOLAR ON In this mode the stimulus that is set by the user will be followed by a second stimulus with the same amplitude and duration but with reverse polarity There is no delay between first and second part of the stimulus except IPG is active see below For instance if the user sets a positive stimulus of 1 V amplitude and 100 us duration this stim
10. ty to full After the battery pack is charged completely the charger changes into a maintanance charge mode so that the battery pack will not be damaged Therefore it is not necessary to disconnect the charger from mains after charging 19 completed ISO 01B npl electronic GmbH D 71732 Tamm Germany BATERY CHARGER Ta wey NA WCE FUSE SLOW gt it 1 25A POWER ON CAUTION FOR USE WITH ISO 018 BATTERY SUPPLY ONLY Figure 5 Front panel left and rear panel right view of the ISO STIM 01B battery charger The battery charger is connected to mains with a standard power cord It has a POWER ON switch on the rear panel Important Only use the supplied charger to charge the ISO STIM O1B battery pack Using different chargers might result in damage of charger and or battery pack Important This battery pack is designed for use with the ISO STIM O1B and the supplied battery charger only Using it differently might result in damage of the battery pack version 1 2 page 10 ISO STIM 01B User Manual 5 Power supply optional Instead of the battery see chapter 4 pack the ISO STIM 01B can be powered with a special power supply see Figure 6 The power supply is connected to the ISO STIM O1B with the provided 9 line SubD cable It will only fit in one direction S npl electronic GmbH ISO 01B D 71732 Tamm Germany POWER SUPPLY MAINS AC www npielectronic com G 100 240V 50 60 Hz T
11. ulus will be followed immediately by a negative stimulus of 1 V and 100 us duration BIPOLAR with IPG In bipolar mode a delay between the first and the second inverse stimulus can be inserted the interphase gap IPG see Figure 3 IPG time is set by 16 interohase gap Figure 3 Interphase gap IPG OPERATION IN THE 10 mA max RANGE According to Ohm s law with 100 V voltage 10 mA current can be achieved only if the resistance of the stimulus electrode is not higher than 10 kQ Otherwise the current will be less version 1 2 page 9 ISO STIM 01B User Manual 4 Battery Pack and Charger The ISO STIM QIB can be powered either with a power supply see chapter 5 or with a battery pack The battery pack is connected to the ISO STIM O1B with the provided 15 line SubD cable It will only fit in one direction A fully charged battery pack will supply the ISO STIM O1B for about 8 h ISO BATT 01B BATTERY POWER SUPPLY npi Figure 4 Front panel view of the ISO STIM QIB battery pack To charge the battery pack disconnect the ISO STIM OIB from the cable and connect the open side of the cable to the charger When the charger is powered on see Figure 5 right a dual color LED on the front panel see Figure 5 left will indicate the status of the battery pack green fully charged red loading There are two charge speeds selectable with a switch on the front panel normal 12 h from empty to full and fast 6 h from emp
12. version 1 2 page 4 ISO STIM 01B User Manual 3 ISO STIM 01 B 3 1 System Description The ISO STIM 01B stimulus isolation unit is designed for application of extracellular stimuli e g in brain slices The output signal is optically isolated from ground and can either be a constant voltage up to 100 V or a constant current up to 10 mA A TTL signal at the TRIGGER INPUT generates one stimulation pulse with adjustable pulse length amplitude and polarity An additional DIRECT INPUT allows the pulse length to be externally controlled whereas amplitude and polarity are controlled by the device itself For both timing modes the generated pulses can be unipolar or bipolar In bipolar mode an interphase gap IPG of adjustable length can be inserted between the two pulses 3 2 Description of the Front Panel YQ G Q 000G ISO STIM 01B STIMULUS ISOLATOR DURATION ms OPERATION MODE AMPLITUDE UNIPOLAR OVER SIM OVER O S s AK pp O O 3 8 g S ea GS X y A K 5 a 4 TRIGGER ee N a iim Zim T H KW Y WW T Ga J a L oy CY D T Ko l IPG TIME OUTPUT Nv Yee ey us FULL SCALE REMOTE TRIGGER INPUT DIRECT INPUT ISOLATED OUTPUT CHASSIS TRIGGER TL TTL CAUTION HIGH VOLTAGE 20 Oe DQ 09 O 408 O OW Figure front panel view of ISO STIM 01B 1 POWER LED LED indicating that the ISO STIM 01D is powered on 2 POWER ON OFF switch Switch to turn POWER of the ISO STIM 01D ON or O
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