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VWR® UltRapURe WateR systems
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1. VWR COLLECTION PRODUCTS DELIVERING HIGH QUALITY amp SUPERIOR PERFORMANCE VWRINTERNATIONAL 9
2. Source Tap water Conductivity uS cm 0 067 0 1 0 067 0 1 0 067 0 1 pHrange o AN Temperature CQ 2 35 Pressure psi ba 1 6 Ordering information j wee a Model TU 3 TU 6 TU 12 Technical specifications Water production rate RO I h 3 6 12 ate Reservoir 6 reservoir built in External tank required for type 2 water for type 2 water sees WxDxH cm 40x30 5x54 5 33x37x61 5 0 06 Weight kg Approx 22 Approx 22 Approx 22 34 NPT Cat No 2 35 Basic 171 1134 171 1137 171 1162 UV 171 1135 171 1138 171 1163 UV UF 171 1136 171 1139 171 1164 Accessories and consumables Description Cat No Replacement UV lamp for Puranity TU PU 15 171 1108 Polisher cartridge for Puranity TU 171 1141 Pre treatment cartridge for Puranity TU 3 171 1142 Pre treatment cartridge for Puranity TU 6 171 1143 Pre treatment cartridge for Puranity TU 12 171 1165 Sterile vent filter for 6 internal tank 171 1144 Sterile end filter 171 1105 Accessories and consumables for TU 12 only Choose 1 storage reservoir Recommended accessories Sterile vent filter for storage tanks and sterile overflow Options UV lamp assembly for storage reservoir wall holder Storage reservoir 30 171 1170 Storage reservoir 60 171 1171 Sterile vent filter for storage reservoirs sterile filter 0 2 um CO adsorber 171 1166 Sterile overflow valve for tanks 30 60 171 1157 UV lamp assembly 171 1188 Replacement UV lamp for external storage tanks 171 1168 Wall holder 171 1125
3. 200 I day Type 1 system pre treated feed lt 50 I day Puranity TU For lt 100 I day Type 1 and Type 2 water from tap water as a feed water source From tap to Type 2 or Type 1 ultrapure water All in one The TU system is a compact system that purifies tap water into both ASTM Type and II water The Puranity TU 3 and 6 feature a built in 6 reservoir to store the Type 2 water The Puranity TU 12 offers the choice of a 30 or 60 external reservoir for customisable storage capacity Puranity TU 3 6 and 12 systems produce up to 100 I day of Type 1 ultrapure water from tap water as well as Type 2 water available from the tank Puranity TU 3 6 and 12 systems include an RO pre treatment cartridge polisher cartridge pack 0 2 uM final filter UV lamp for UV systems only and ultra filter for UF systems only The 3 and 6 h systems also include an internal 6 tank with vent filter The TU 12 requires an external tank 4 VWRINTERNATIONAL VWR COLLECTION PRODUCTS DELIVERING HIGH QUALITY amp SUPERIOR PERFORMANCE Typical applications include Molecular biology and microbiology e Cell and tissue culture e PCR DNA sequencing e Electrophoresis Analytical chemistry e HPLC e GC GC MS ICP MS AA e TOC measurements IC Feed water requirements Source Potable tap water Puranity PU 15 Type 1 system produces up to 50 I day of 18 2 MQxcm water at 25 C Smart features e Backlit display tilts for easy
4. VWR ULTRAPURE VWRES We Enable Science WATER SYSTEMS TA Great performance up to 200 a day vanes ro Puranity models offer flexibility for diverse applications Systems delivered with free consumables and accessories Easy to install and maintain 2 year warranty VWR ULTRAPURE WATER SYSTEMS Water is the most basic and yet the most critical component of any experiment While tap water may be good enough to drink laboratory methods are more sensitive than human physiology VWR water purification systems remove the impurities that threaten your results from simple glass rinsing to solution production or instrument feeds These are some of the challenges managed seamlessly by the VWR Puranity range Table 1 Specific areas of concern by application Application Particulates Colloids lons Dissolved gases Organics Nucleases Pyrogens Table 1 shows the impact of Ceperollaboralory different contaminating parameters AE on experimental performance by Humidification a lication Glassware washing rinsing pp Media preparation e e There are 2 major classes of impurities in AED eee water dissolved and or undissolved lon chromatography IC os 3 3 impurities For both classes there exists a Atomic absorption AA High performance liquid different and optimal method of chromatography HPLC purification Inductively c
5. e bar 2 to 6 Electrical requirements 90 240 V 50 60 Hz Power consumption kW 0 06 Feed water connector 3 4 NPT WxDxH cm 30 5x30x54 5 Weight kg Approx 17 Temperature min max C 2 35 biology PCR DNA such as AAS IC ICP methods such as HPLC monoclonal antibodies ICP MS TOC analyses cell culture media Resistance at 25 C MQ cm 18 2 18 2 18 2 Conductivity uS cm 0 055 0 055 0 055 TOC ppb 5 10 1 5 1 5 RNase ng ml n a n a lt 0 003 DNase pg ul n a n a lt 0 4 Bacteria CFU ml lt 1 lt 1 lt 1 Particles 0 22 um ml lt 1 lt 1 lt 1 Endotoxins EU ml n a n a lt 0 001 Flow rate l min 1 5 1 5 1 Cat No 171 1100 171 1102 171 1101 Accessories and consumables Disinfection cartridge 171 1182 Cleaning solution 1 syringe disinfection cartridge required 171 1124 Disinfection granulate micro chlor VE12 171 1123 disinfection cartridge required Polisher cartridge for Puranity PU 15 171 1104 Replacement UV lamp for Puranity TU PU 15 171 1108 Sterile filter 0 2 um 171 1105 Ultra filter for Puranity PU 15 TU 3 6 12 171 1106 Wall holder for Puranity TU PU 15 171 1125 VWR COLLECTION PRODUCTS DELIVERING HIGH QUALITY amp SUPERIOR PERFORMANCE VWRINTERNATIONAL 5 VWR ULTRAPURE WATER SYSTEMS Puranity PU 20 Type 1 system producing up to 200 I day 18 2 MQxcm water Exceeds International Standard ASTM Type I ISO 3696 Grade 1 ASTM D1193 and CLSI CLRW Typical applications include Mo
6. lecular biology and microbiology e Cell and tissue culture e PCR DNA sequencing e Electrophoresis analytical chemistry e HPLC GC GC MS ICP MS AA e TOC measurements IC Integrated feed water monitoring e Built in feed water cell monitors feed water conductivity any exceeding of the limiting value is immediately displayed maximising cartridge life UV photo oxidation 185 254 nm e High performance UV assembly reliably reduces the content of microorganisms and their metabolites e UV oxidation also reduces organic compounds in the water to ultra low levels e Ready to use e Feed water pressure regulator wall bracket UV lamp ultrapure polisher cartridge sterile filter all included with one part number Measurement of conductivity resistivity e Conductivity cells are carefully calibrated prior to each measurement via built in reference resistance with cell constants at 0 01 cm 1 e Temperature measurements are made by a platinum chip sensor with 0 1 C accuracy Quick connect cartridge replacement e Aquastop quick connect capability enables cartridge replacement within seconds even mid operation GLP compliant documentation e Real time clock and code protected operating system prevents unauthorised changes to system settings RS232 interface with adjustable send interval for safe data transfer of all measured data faults date and time to a PC or log printer Digital microprocessor control automatically monit
7. me synergistically to remove impurities and give you consistently PURE water Water purification is a step by step process often requiring a combination of technologies Table 2 Efficiency of different methods at removing contamination from water Inorganic ions Dissolved gases Organics Particles Bacteria Pyrogens Nucleases Distillation Reverse osmosis Deionisation Electrodeionisation Filtration Ultrafiltration UF Adsorption Ultraviolet oxidation UV Combination UV UF 4 Key Excellent Good Poor The choice of methodology used depends on the application the water is being used for In general the higher the purity the more expensive it is to produce every litre Table 3 International Standards for water quality Type of water Electrical conductivity Salt content ppm TOC content pg l pS cm Tap water 600 300 Up to 5000 ASTM Type 3 0 25 0 05 200 ASTM Type 2 1 0 12 50 ASTM Type 1 0 055 0 028 50 Tap water quality differs from place to place this is an example only Typically Type 2 or RO water is used for general laboratory equipment like water baths or incubators buffer media and reagent preparation or as a feed water supply for Type 1 water purification syste
8. ms Type 1 water is essential for analytical and life science techniques to ensure the best results VWR COLLECTION PRODUCTS DELIVERING HIGH QUALITY amp SUPERIOR PERFORMANCE VWR INTERNATIONAL 3 VWR ULTRAPURE WATER SYSTEMS Overview of VWR Puranity water purification systems Elegant simplicity performance and value The VWR Puranity range offers 5 different models of purification systems to meet your needs without compromise Each model uses an internal recirculation mode to ensure availability of high purity water at any time Exchanging ultra polisher cartridges and pre treatment cartridges in TU models is easy without requiring any system depressurisation Each system is available in 3 different models Basic with UV lamp UV or UV lamp and ultrafilter UV UF to fullfil the specific demand of each application Systems requiring pre treated feed Puranity PU 15 and 20 systems include a polisher cartridge pack 0 2 um final filter pressure regulator feed water monitoring and optional UV lamp and ultra filter Puranity PU 15 Designed for laboratories needing up to 50 I day of ultrapure water such as HPLC or other instrumental analyses sample preparation glassware rinsing or molecular biology work where endotoxins and or nucleases would interfere Puranity PU 20 The Puranity PU 20 system delivers ultrapure water to labs with a higher volume demand than the PU 15 systems Type 1 system pre treated feed lt
9. nation by microorganisms Recirculation pump protects the high purity water from bacterial growth during standstills and maintains low conductivity value Lasting economy e Water purification progresses through independent cartridges with Aquastop and quick connect for fast replacement e Module 1 Combination of pre treatment and the reverse osmosis membrane e Module 2 Polishing cartridge contains high quality ultrapure resin for consistent purity and long cartridge life Easy to operate e Dispensing is easy and features variable speed to control flow e The display can be tilted for optimal reading e Place on bench or mount onto the wall 8 VWRINTERNATIONAL VWR COLLECTION PRODUCTS DELIVERING HIGH QUALITY amp SUPERIOR PERFORMANCE Model TU TU UV TU UV UF Applications AAS IC ICP Inorganic and organic Molecular biology PCR DNA standard buffer trace analysis HPLC monoclonal antibodies cell ICP MS IC TOC analyses culture media Type 1 water Resistance at 25 C MQ cm 18 2 18 2 18 2 Conductivity uS cm 0 055 0 055 0 055 TOC ppb 5 10 1 5 1 5 RNase ng ml n a n a lt 0 003 DNase pg ul n a n a lt 0 4 Bacteria CFU ml lt 1 lt 1 lt 1 Particles 0 22 um ml lt 1 lt 1 lt 1 Endotoxins EU ml n a n a lt 0 001 Flow rate l min Up to 1 Up to 1 Up to 1 Type 2 water d Pure water production at 15 C l h 3 6 or 12 3 60r 12 3 60r 12 Feed water requirements Resistivity at 25 C MQ cm 15 10 15 10 15 10
10. ors and stores faults from the last four weeks e USP compliant conductivity measurement with temperature compensation can be switched on or off 6 VWRINTERNATIONAL VWR COLLECTION PRODUCTS DELIVERING HIGH QUALITY amp SUPERIOR PERFORMANCE Tilting control panels for easy viewing e Ergonomically designed controller tilts for optimal key pressing and easy readability e Illuminated four line alphanumeric display Model PU 20 PU 20 UV Applications AAS IC ICP standard buffer analysis HPLC ICP MS IC TOC analyses Resistance at 25 C MQ cm 18 2 18 2 Conductivity uS cm 0 055 0 055 TOC ppb 5 10 1 5 RNase ng ml n a n a DNase pg ul n a n a Bacteria CFU ml lt 1 lt 1 Particles 0 22 um ml lt 1 lt 1 Endotoxins EU ml n a n a Flow rate l min Up to 2 Up to 2 Cat No 171 1172 171 1174 Feed water requirements ion exchange or distillation lt 2 Max 50 lt 100 lt 1 0 2 35 1 4 87 0 1 6 Technical specifications 0 1 to 6 90 240 V 50 60 Hz 0 1 3 4 NPT 37x33x61 5 22 24 2 35 Accessories and consumables Disinfection cartridge for Puranity PU 20 171 1182 Cleaning solution 1 syringe disinfection cartridge required 171 1124 Disinfection granulate micro chlor VE12 171 1123 disinfection cartridge required Polisher cartridge for Puranity PU 20 171 1175 Replacement UV lamp for Puranity PU 20 UV UV UF 171 1176 Ultra filter for Puranity PU 20 171 1184 Sterile filte
11. oupled l plasma spectroscopy ICP Dissolved impurities are inorganic ions Mass spectroscopy MS i small organic molecules as well as Gas chromatography GC e e l Total organic carbon TOC bigger more complex molecules for Life science laboratory instance absence of nucleases bacterial sone endotoxins and pyrogens are very e g PCR mutagenesis f i S important for experiments in life science e g crystallography o o labs and gases electrophoresis o inanod Unsolved impurities are suspended e g monoclonal antibody o particles sand rust and more production blots i l microorganism like bacteria fungi algae Pharmacology Cell and tissue culture e and Viruses and last but not least Drug discovery i colloids which can cause indefinable results in some applications Suspended particles colloids and inorganic ions Suspended particles typically 1 10 um in size include sand silt etc that can interfere with instrument operation plug valves and other narrow flow paths as well as foul reverse osmosis membranes Slightly smaller 0 01 1 0 um colloidal particles typically have a slightly net negative charge and clog filters interfere with instrument operation foul reverse osmosis membranes and can bypass ion exchange resins resulting in lower re
12. r 0 2 um 171 1105 Inorganic and organic trace Potable tap water pre treated by reverse osmosis PU 20 UV UF Molecular biology PCR DNA monoclonal antibodies cell culture media 18 2 0 055 1 5 lt 0 003 lt 0 4 lt 1 lt 1 lt 0 001 Up to 1 5 171 1173 VWR COLLECTION PRODUCTS DELIVERING HIGH QUALITY amp SUPERIOR PERFORMANCE VWR INTERNATIONAL 7 VWR ULTRAPURE WATER SYSTEMS Puranity TU 3 6 12 For lt 100 I day Type 1 and Type 2 water from tap water as a feed water source Typical applications include Molecular biology and microbiology e Cell and tissue culture e PCR DNA sequencing e Electrophoresis S gt Analytical chemistry e HPLC e GC GC MS ICP MS AA e TOC measurements IC Routine laboratory work Preparing and diluting buffers and reagents Rinsing lab glassware Supplying autoclaves and other small lab equipment Powerful performance e Based on your requirements choose from capacities of 3 6 or 12 I h e Conical bottom outlet allows for complete draining and efficient cleaning and disinfection Puranity TU 3 and 6 feature smart integrated 6 reservoir Puranity TU 12 offers external reservoir options either 30 or 60 Polyethylene reservoir is opaque to light CO adsorber prevents TOC value increase from drawn in CO Optional UV lamp and ultra filter to customise ultrapure water quality Sterile venting Tilter reservoir overflow protects against contami
13. sistivity in deionised water systems Cations and anions adversely affect the results of inorganic analyses such as IC AA ICP MS and may retard cell and tissue growth in biological research They can also affect cartridge life in deionised water systems 2 VWRINTERNATIONAL VWR COLLECTION PRODUCTS DELIVERING HIGH QUALITY amp SUPERIOR PERFORMANCE Dissolved gases and organics Water naturally contains dissolved gases such as oxygen or carbon dioxide and others that can alter the pH of the water and oxygen the most common non ionised gas may cause corrosion of metal surfaces While the exact source and composition may vary organics foul ion exchange resins interfere with organic analyses including HPLC gas chromatography and fluoroscopy As well as hindering electrophoresis tissue and cell culture Typical organic impurities in tap water are proteins residues of pesticides as well as more and more hormones due to the inability of wastewater plants to remove them Biologicals Although chlorination eliminates harmful bacteria tap water still contains live microorganisms along with pyrogens viruses and nucleases that interfere with Sterile applications such as cell and tissue culture and can have a devastating impact on many life science research protocols Purification methods To make pure and ultrapure water impurities need to be efficiently and effectively removed Water purification systems employ multiple technologies so
14. viewing e Variable speed dispensing e Easy to change polisher cartridge e Small footprint Monitor feed water quality e Good feed water quality leads to consistently ultrapure water and hence maximum cartridge life Smart design e Recirculation pump prevents bacterial growth during standstill and allows for constant monitoring of all system functions allowing for the immediate display of fault messages if a problem is detected e System delivered fully equipped with pressure regulator ultrapure polisher cartridge and 0 2 um sterile filter Superior filters e Ultra filter is flushed automatically to ensure the highest retention of endotoxins and nucleases which produces a long two year lifetime e Validated 0 2 um final filter with folded membrane can be sterilised up to 5 times UV photo oxidation 185 254 nm e High performance UV assembly reliably reduces the content of microorganisms and their metabolites e UV oxidation also reduces organic compounds in the water to ultra low levels Model PU 15 PU 15 UV PU 15 UV UF High sensitivity Applications Instrumental Microbiology molecular analytical methods instrumental analytical pre treated by reverse osmosis ion exchange or distillation Feed water conductivity uS cm lt 5 TOC ppb Max 50 Turbidity NTU lt 1 0 Temperature C 2 35 Pressure psi bar 1 4 87 0 1 6 See user manual for more details Technical specifications Operating pressur
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