RAT BRAIN CORTEX NEURONS
Contents
1. www innoprot com2. THESE PRODUCTS ARE FOR RESEARCH USE Recommended Medium ONLY Not approved for human or veterinary e Neuronal Medium Gerum free use for application to humans or animals or Reference P60157 for use in vitro diagnostic or clinical procedures INNOVATIVE TECHNOLOGIES IN BIOLOGICAL SYSTEMS S L Parque Tecnol gico Bizkaia Edf 502 12 Planta 48160 Derio Bizkaia Tel 34 944005355 Fax 34 946579925 innoprot innoprot com www innoprot com innoprot Speeding up drug discovery INSTRUCTIONS FOR CULTURING CELL IMPORTANT Cryopreserved cells are very delicate Thaw the vial in a 37 2C waterbath and return them to culture as quickly as possible with minimal handling 1 For cryopreserved cells If there is dry ice in the package and you are not going to culture cells right way place 3 Set up culture Prepare one T 25 or M96 flask for each cryovial Add the appropriate amount of medium to cryovial s immediately into liquid nitrogen If there is no dry ice left in the package thaw and culture the cells immediately 2 For proliferating cells Spray the culture vessel flask plate or slide with 70 ethanol for disinfection Transfer the cells into 372C 5 CO incubator and allow equilibrating for 2 hours After cells have equilibrated remove shipping medium from the culture vessel and replace with fresh medium the vessel recommend for 4 ml T 25 flask or 200 ul per well for M96 and allow t
3. For best result do not disturb the culture for at least 16 hours after the culture has been initiated Change the growth medium the next day to remove the residual DMSO and unattached cells then every other day thereafter A health culture will display normal neuron morphology and nonvacuole cytoplasm with multiple processes INNOVATIVE TECHNOLOGIES IN BIOLOGICAL SYSTEMS S L Parque Tecnol gico Bizkaia Edf 502 12 Planta 48160 Derio Bizkaia Tel 34 944005355 Fax 34 946579925 innoprot innoprot com www innoprot com innoprot Speeding up drug discovery Cautions Handling animal derived products is potentially bioharzadous Although each cell strain testes negative for microbial diagnostic tests are not necessarily 100 accurate therefore proper precautions mush be taken to avoid inadvertent exposure Always wear gloves and safety glasses when working these material Never mouth pipette We recommend following the universal procedures for handling products of human origin as the minimum precaution against contamination 1 1 Grizzle W E and Polt S S 1988 Guidelines to avoid personal contamination by infective agents in research laboratories that use human tissues Tissue Culture Methods 11 4 INNOVATIVE TECHNOLOGIES IN BIOLOGICAL SYSTEMS S L Parque Tecnol gico Bizkaia Edf 502 12 Planta 48160 Derio Bizkaia Tel 34 944005355 Fax 34 946579925 innoprot innoprot com
4. he flask to equilibrate in 372C 5 CO incubator for at least 30 min 4 Thawing of cells Place the vial in a 372C waterbath hold and rotate the vial gently until the contents are completely thawed Remove the vial from the waterbath immediately wipe it dry and transfer it to a sterile field Rinse the vial with 70 ethanol and then wipe to remove excess Remove the cap being careful not to touch the interior threads with fingers 5 Transfer the cells with the freezing medium to 15 ml tube and add 10 ml of culture medium Centrifuge at 230 g during 5 minutes Discard the supernatant Resuspend the pellet in the suitable volume of medium and et up culture after receiving the orders 1 Coat culture vessel with poly L Ornitine Note It is important that neurons are plated in poly L lysine or poly L Ornitine coated culture vessels that promote cell attachment and neurites outgrowth coat flask or plate with poly Ornitine at 3 ul ml concentration for one hour and wash the flask or plate with sterile water three times 2 Medium preparation Decontaminate the external surfaces of medium and medium supplements with 70 ethanol and transfer them to sterile field Aseptically open each supplement tube and add them to the basal medium with a pipette Rinse each tube with the medium to recover the entire volume culture at 372C 5 CO in the incubator A higher seeding density gt 10 000 cm is recommended 6
5. innoprot Speeding up drug discovery NEUROSCIENCES INNOPROFILE RAT BRAIN CORTEX NEURONS Product Type Cryo preserved Brain Cortex Neurons Catalog Number P10102 Source Day 18 embryonic Sprague Dawley Rat Brains Format gt 1 x10 cells in Cryopreserved vials Storage Liquid Nitrogen Rat Neurons RN from Innoprot are isolated from 18 days gestation rat embrions RN are cryopreserved at secondary cultures and delivered frozen Each vial contains gt 1 x 10 cells in 1 ml volume RN are characterized by immunofluorescent method with antibodies to neurafilament MAP2 RN are guaranteed to further culture in the conditions provided by Innoprot Fig 1 Inmunofluorescent staining of passage two rat hippocampal neurons with anti MAP 2 antibody in green neuronal marker and DAPI in blue 20X The tissue of the central nervous system is made up of two classes of cells that may be Product Characterization broadly categorized as neurons and glia Neurons are anatomic functional and trophic Immunofluorescent method units of the brain Despite great variabilty in o Neurafilament size and shape all neurons share common o MAP2 morphologic features which are those of the Negative for mycoplasma bacteria yeast and key elements of a highly complex fungi communication network The neurons are the dynamically polarized cells that serve as the major signaling unit of the nervous system Product Use
Download Pdf Manuals
Related Search