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Leica M Stereo

1. planapo objectives Part no MS5 26 MZ75 MZ95 2125 MZ16 MZ16 A Achromat Achromat 1x 10 411 589 C C C C Achromat 1 5x 10 422 562 C C C C Achromat 2x 10 447 081 C C C C Achromat 0 8x 10 473 832 C C C C Achromat 0 63x 10 445 201 C C C C Achromat 0 5 10 422 563 C C C C Achromat 0 32 10 422 564 C C C C Ergo objective 0 4x 0 63x 10 447 148 H C C C Planachromatic Planachromatic 1x 10 446 275 C C C C Planachromatic 1x MZ125 MZ16 10 445 819 10 446 172 10446172 10446172 Planachromatic 0 5x 2125 216 10 446 157 10 446 172 10446172 10446172 C Planachromatic 0 8x 10 447 075 10 446 172 CA 10446 172 10446 172 C M C Planapochromatic Planapochromatic 1x 2125 216 10447 157 10 446 172 CA 10446 172 10 446 172 M C C Planapochromatic 1 6x MZ125 MZ16 10 447 050 10 446 172 CA 10446 172 10446172 C C Planapochromatic 0 63x MZ125 MZ16 10 447 051 10 446 172 10 446 172 10 446 172 C C Planapochromatic 2x MZ125 MZ16 10 447 101 10 446 172 CA 10446 172M 10 446 172 M C M C C Accessories Coaxial incident light 10 446 180 0 10 446 300 0 10 446 300 C C Microscope carrier AX 10 445 618 C C 55 26 275 295 Microscope carrier 10 447 062 CA CA CA C C C MZ125 MZ16 MZ16 A Near v
2. with incident light stand with transmitted light stand 216 216 OO 1x Plan IL 1 Plan 00 1 n p q HH gt p e 3 222 222 Measurements mm 1 275 I a 275 O Na 5 y x 9 a 2 i a o Cami O 1 Y I Y Y vy 7 120 gl 80 2 120 i I 2 s Sg lt Y Y 400 Additional dimensional diagrams involving Leica ErgoModule are available from your Leica agency Leica M Stereomicroscopes User Manual 69 Dimensions of Leica MZ95 with incident light stand with transmitted light stand 216 5 1 ELI LAH J D LAU 222 Measurements mm ST 1 295 1 295 1 O sn Yn p ks N o lt e 1 P 3 Y I Y Y 7 120 80 2 120 285 320 294 328 2 DI 4 e 0 461 4 Additional dim
3. Y 9 2 1 5 19 C O 4 15 co EN di Y 1 Y Y 71 120 e 70 2 120 Additional dimensional diagrams involving Leica ErgoModule are available from your Leica agency 72 Leica M Stereomicroscopes User Manual Dimensions of Leica MZ16 A with incident light stand with transmitted light stand 104 SZ Z ay 912 1 1x Planapo 1 Planapo 10 50 358 503 222 8 388 8 325 Additional dimensional diagrams involving Leica ErgoModule are available from your Leica agency Leica M Stereomicroscopes User Manual 73 Technical data electronic devices Transmitted light base 20 W Technical Data Type description Transmitted light base 20 W Power supply ES 635 474 incl potentiometer Type of bulb Halogen bulb 6 V 20 W Socket Connection for one external incident illumination with a maximum combined loading of 20 W Operating voltage Primary voltage 115 230 V 50 60 Hz Secondary voltage 7 V 28 VA Fuses primary For 115 V 315 m AT for 230 V 160 m AT Switch positions Position I Transmitted light 6 V 20 W Position Il External incident lamp
4. gt Shift the stereomicroscope to the central position Leica M Stereomicroscopes Operation Axial observation Aperture The parallax free imaging produces more precise results in photography measuring and polarization gt Shift the stereomicroscope to the left or the right position The object is now located directly beneath either the left or the right beam path When photographing or measuring se lect the beam path above which the pho totube or measuring graticule is located A rotating aperture is build into the AX microscope carrier It prevents interfering reflections during axial observation with coaxial incident illuminator and quarter wave plate e With the AX microscope carrier for 55 26 MZ7s 295 the aperture automatically turns during the moving process e With the AX microscope carrier for MZ125 MZ16 MZ16 A the aperture is located underneath the microscope carrier and must manually be turned as follows Please note Inthe position for stereoscopic observa tion through both beam paths turn the aperture counterclockwise until it stops Otherwise the field of view is underex posed vignetting nthe position for axial observation through the right or left beam path turn the aperture clockwise until it stops Leica M Stereomicroscopes Operation 19 20 Tips for your work I A Preparations B Focus on the test object Ad
5. 3 1 2 17 Leica DC cameras photoautomats CCD camera CCD camera CCD camera 11 541 038 10446 120 B 0 55x 1 1x e 10 162 226 10 446 261 10 445 541 2 3 0 63x 11 541 006 10 446 175 10 404 207 1 1x 10 445 929 10 445 928 1 2 0 5x 11 543 703 11543 702 11543 706 11541 007 10 445 932 11541 016 1 2 0 5x 10 445 930 1 10 446 121 8 10 446 131 10x 10 446 132 16x HD F 10 446 308 amp HD V 10 446 309 lt 10 445 924 50 10 446 229 100 Leica MZ16 MZ16 H HD50 10 445 931 10 446 197 10 445 925 Leica MS5 MZ6 MZ7s MZ9s m d 7125 MZ FLIII 10 445 927 52 Leica M Stereomicroscopes Accessories Partition of light Stop Video phototubes are designed to support modular photomicrography systems and Leica digital video film or SLR cameras The modular photomicrographic systems and the Leica digi tal cameras are detailed in a separate user manual along with instructions for their assembly and use When you are not taking photographs engage the observa tion beam path Accessories such as the coaxial incident illuminator always fit between the stereomicroscope and the phototube Video phototube A This tube does not permit stereoscopic observation The image is studied either in the camera viewfinder or down the focusing telescope of the camera In the video photo beam path 10
6. Attach the limit stop to the stand column It prevents the 2x objective from hitting the limit stop when the objective nosepiece is rotating if the work ing distance was changed xwhile working with the 1 objective Caution Carefully rotate the objective nosepiece and take care not to pinch your fingers between column and objective or objective nosepiece MZ16 A When changing to objective 1x or 2x press the OPT SEL function keys simultaneously The display shows the current total magnification and in REF mode it shows the current measurement with objective 1x or 2x 1 2 in front of the value indicates the selected objective Remove the securing strap from the objective nosepiece gt Remove both screws at the bottom Leica MZ16 and MZ16 A are delivered with a threaded adapter for objectives Remove the adapter to use the objective nosepiece gt Invert the optics carrier gt Loosen 3 screws and remove the adapter The objective nosepiece features two threads a fixed thread for the 2x planapochromatic objective athread with 2 Allen screws for the 1x planapochromatic objective with adjustable ring nut The adjustable ring nut is supplied separately and used to adjust the parfocality between both objectives Invert the objective nosepiece to simplify the assembly of the objectives Screw on the adjustable ring nut on the thread of the 1x planapochromatic objective until the limit stop and tighten it wi
7. Leica M Stereo microscopes English User Manual MICROSYSTEMS Dear User User Manual Thank you for choosing our products We hope that you will en joy the quality and performance of Leica Microsystems products In developing our instruments we have placed great emphasis on simple self explanatory directions In order to utilize all the benefits of your new stereomicroscope we suggest studying this user manual in detail Should you have any questions please consult your local Leica representative You will find the address of the closest local representative as well as valuable informa tion about products and services from Leica Microsystems on our homepage at www leica microsystems com We are gladly at your service Customer service is a big thing with us Not only before the sale but afterwards as well Leica Microsystems Switzerland Ltd Stereo amp Macroscope Systems www stereomicroscopy com Your instrument is accompanied by a printed English user man ual Additional language versions and information can be found on the interactive CD ROM User manuals and updates are available for download on our homepage at www stereomicroscopy com This user manual contains an explanation of the safety regula tions assembly handling and accessories of the Leica MS5 MZ6 275 295 MZ125 MZ16 MZ16 A and MZ16 if identical stereomicroscopes The special functions of the automated stereom
8. Power cables Cable routing Opening the instrument Supply voltage Connections Changing bulbs Electrically operated items The safety regulations listed below apply to the following items Transmitted light stand bright field 20 W page 24 Supply line lamp 25 W page 47 Regulating transformer 0 7 V 40 W page 38 Step transformer 4 5 6 V page 39 Motor focus separate instructions Motorized Leica MZ16 A Be careful when handling liquids If spilt on the equipment they can cause the stereomicroscopes and accessories to become electrically live endangering personnel cause damage to the equipment Inspect cables regularly for damage Defective cables can directly endanger personnel cause the stereomicroscopes and accessories to become electrically live endangering personnel Route cables with care Make sure that personnel do not get entangled with them The instrument could fall over fall down become damaged damage other equipment or cause personal injury Only authorized Leica personnel are permitted to repair electri cal equipment Before opening up the equipment pull out the power cable If you touch the live interior of the equipment you may receive an electric shock Ensure that the voltage setting on the equipment is correct A wrong setting can cause damage to the equipment When connecting power consuming devices to the transform ers do
9. 179 20 112 597 38 32 512 44 64 35 1024 22 4 55 32 1 16 14 256 88 764 29 4 64 35 80 28 128 18 512 44 40 56 20 112 32 7 955 23 5 80 28 100 22 160 64 35 50 45 25 9 40 58 1184 19 6 96 23 120 19 192 12 768 29 60 37 30 15 48 1433 16 0 63 158 15 197 121 315 75 126 188 98 242 49 485 79 301 235 101 0 8 20 119 25 95 40 59 16 148 125 19 63 377 10 238 299 73 1 25 95 313 76 50 48 20 119 156 152 78 304 125 19 373 64 125 313 76 391 61 625 38 25 95 195 122 98 242 156 152 466 51 16 40 59 50 48 80 3 32 74 25 95 125 19 20 119 597 4 25x 9 5B 2 50 625 100 24 40 59 313 76 156 152 25 95 746 32 25 625 38 781 125 19 50 48 391 61 195 122 313 76 933 25 32 80 3 100 24 160 15 64 37 50 48 25 95 40 59 194 2 4 100 24 125 19 200 12 80 3 625 38 313 76 50 48 1493 1 6 5 125 19 1563 15 250 1 100 24 781 3 381 61 625 38 186 6 13 6 150 16 1875 13 300 08 120 2 938 25 469 51 75 32 2239 11 0 63 252 95 315 76 504 48 202 119 158 152 79 304 126 19 378 64 0 8 3 75 0 6 64 38 256 94 20 12 10 24 16 15 478 5 1 40 6 50 48 80 3 32 75 25 96 125 192 20 12 597 4 1 25 50 48 625 38 100 24 40 6 313 77 156 154 25 96 746 32 16 64 38 80 128 19 512
10. 216 66 Dimensions 67 Technical data electronical devices 74 Leica M Stereomicroscopes User Manual 5 Safety concept General directions Permitted uses Prohibited uses Place of use Use in ESD protected rooms Use in Clean Rooms Read the user manual and safety instructions before starting up The Leica 55 MZ6 MZ7s MZ9s MZ12s MZ16 and MZ16 A stereomicro scopes are optical instruments which use magnification for improved visualization of objects their details and specimens from technology and natural sciences The basic outfit which includes a stand and a light source can be supplemented with various modules for photography TV dual station viewing and other applications The use ofthe instrument in a different manner from that described in this user manual can lead to injury malfunction and damage e The Leica 55 MZ6 MZ7s MZ9s MZ12s MZ16 and MZ16 A stereo microscopes must not be used for eye examinations and operations Do not fit different plugs Do not dismantle optical systems mechani cal parts unless instructions for doing so are given in the user manual e The Leica 55 MZ6 MZ75 MZ9s MZ125 MZ16 and MZ16 A stereo microscopes are intended mainly for indoor use fthe stereomicroscope is used outdoors protect it from dirt and mois ture Lamps and electrically operated stands from Leica ma
11. 47 40 6 20 12 3 75 955 25 40x 6B 2 80 3 100 24 160 15 64 38 50 48 25 96 40 94 2 25 100 24 125 19 200 12 80 3 625 38 33 77 50 48 1493 1 6 32 128 19 160 15 256 09 1024 23 80 3 40 6 64 38 191 13 4 160 15 200 12 320 08 128 19 100 24 50 48 80 2388 1 5 200 12 250 1 400 06 160 15 125 19 625 38 100 24 2985 08 6 240 1 300 08 480 05 192 13 150 18 75 3 2 120 2 3582 07 Position 6 for MZ95 only When using the planachromatic and planapochromatic objectives 2125 the magnification is increased by the factor 1 25x 64 Leica M Stereomicroscopes User Manual Ergo ohjective Objectives 0 4x 0 63x Working distance mm s 63 5 mm 153 5mm 5 El 5 Eyepieces 5 s s EISIE 5 s lt 8 8 S z 8 5 8 s 3 s 3 0 63 4 525 26 808 08 5 42 33 636 1 64 328 4 512 125 80 263 5 412 16 102 206 66 318 10x 21B 2 127 165 82 256 25 159 132 103 204 32 204 103 132 159 4 255 82 165 127 5 318 66 206 102 6 382 55 247 85 0 63 64 35 4 546 08 82 773 53 423 1 102 22 66 339 125 127 176 82 273 16 163 137 105 213 16x 14B 2 24 n 132 17 25 255 88 165 136 32 326 69 21 106 4 408 55 263 85 5 51 44 329 68 6 61 37 395 57 0 63 10 238 65 365 08 127 187 82 29 1 159 149 103 23 125 199 119 129
12. N 4 29 Table clamp stand Swinging arm stand Safety ring column Safety ring column 30 Limitation AN Drive housing horizontal arm Large swinging arm and table clamp stands gt Use the clamp to secure the column to a table top 20 mm 50 mm thick gt Insert 4 Allen screws into the base from beneath and secure them to the end of the column The safety ring secures the instrument and must always be positioned beneath the horizontal arm Position the safety ring with the screw head uppermost and towards you and slide it down the column gt Retighten the clamping screw gt Lower the horizontal arm onto the safety ring with its end pointing towards you gt Retighten the clamping screw m The screw head must engage the notch It limits the lateral movement It may not be removed and neither may the safety ring be milled down If the horizontal arm is swung out beyond the limit the instrument may fall over This section describes the assembly of the focusing drive with side faced carrier rod To fit the focusing drive with peg refer to page 28 Release the clamping lever gt Tilt the carrier rod downwards gt Retighten the clamping lever gt Slide the drive housing with side faced carrier rod onto the horizontal arm gt Retighten the clamping lever The clamping levers can be positioned as required gt Pull out the clamping lever along its axis and
13. Turn the rotary knob A movable prism directs the light from the left or right outlet onto the object Leica M Stereomicroscopes Illuminations 49 Accessories Assembly 50 Tubes All accessory tubes which fit between the optics carrier and the binocular tube are fitted in the same manner nthe dovetail ring of the optics carrier is a locating screw which must engage the notch in the accessory page 35 This is best accomplished by moving the accessory slightly from side to side Release the clamping screw gt Insert the accessory tube into the dove tail ring in the same manner as the binoc ular tube page 35 and move it slightly from side to side until the locating screw engages the notch gt Retighten the clamping screw gt Fit the binocular tube on the accessory tube in the same manner Double iris diaphragm A double iris diaphragm is built into the MZ16 and MZ16 A It is operated by means of a knurled ring A separate double iris diaphragm is available as accessory for the other instruments The double iris diaphragm for MS5 MZ125 can be inserted between optics carrier and binocular tube or video phototube Set the lever to OPEN The double iris diaphragm aperture diaphragm is open The depth of field increases as the lever is moved from OPEN to 1 Remember however that brightness decreases so exposure times become longer resolution deteriorates Leica M St
14. field consists of a fiber optic light guide and a light source Observe the safety regulations from the manufacturer of the light source gt Unplug the fiber optic light guide from the connection in the stand gt Fit the side faced column to the transmitted light base as described on page 23 gt Fit the microscope carrier and stereomicroscope as described on page 34 gt Attach the fiber optic light guide at the stand and the light source Before using the light source read the corresponding user manual Bright field is suitable for examining translucent objects featur ing contrasting structures The objectis directly illuminated from below and is seen in its natural colors against a bright background JU gt Slide handle to the right until the stop bright field Dark field offers more information for objects poor in contrast with rather poorly marked or very fine structures In this case the light beams penetrate the object at a flat angle Fine detail structures and contours contrast brilliantly and brightly from dark background gt Slide the handle out to the right dark field Leica M Stereomicroscopes Assembly Column Base Illumination Accessory condenser gt Basis Use Tilted mirror Transmitted light stand HL RCTM Transmitted light stand HL The column seat contains Allen screws gt Remove the glass stage plate gt Invert the transmitted light base and place it on a
15. 21B 16 102 206 66 318 2 127 165 82 256 25 159 132 103 204 32 204 103 132 159 4 255 82 165 127 0 63 64 35 4 546 08 82 273 53 423 1 102 22 66 339 125 127 176 82 273 16x 14B 18 163 137 105 213 2 204 132 17 25 255 88 165 136 32 328 69 2 10 6 4 408 55 263 85 0 63 10 238 65 365 08 127 187 82 29 1 159 149 103 23 125 199 119 129 184 25x 9 5B 16 255 93 165 144 2 318 75 206 115 25 388 6 257 92 32 51 329 72 4 637 37 412 58 0 63 16 149 104 23 08 204 118 132 182 1 255 94 165 145 125 318 75 206 117 40x 6B 18 408 59 9 2 51 329 73 25 637 38 412 58 32 815 29 527 46 4 10 9 24 658 36 Leica M Stereomicroscopes User Manual 63 Optical data of Leica MZ75 MZ95 1x Plan 1 1 6x Planapo 0 63x Planapo 0 5x Plan 0 32x Achromat 0 5x Achromat 1 5x Achromat Objectives 1x Achromat 2 Achromat 0 8xAchromat 0 63x Achromat 0 8x Plan Working distance mm 5 81 Plan 55 Planapo 19 Planapo 97 Planapo 135 Plan 297 Achromat 187 Achromat 49 Achromat 89 Achromat 21 Achromat 112 Achromat 149 Achromat 112 Plan Eyepieces 5 5 s E E 3 E E 8 3 5 E 5 E 5 5 E S E 5 E 5 E 5
16. 4 Power switch 0 off 5 ON display Instrument is switched on Regulating knob 7 6 V display The standard voltage has been reached Technical see page 74 f er Starting Switching the input voltage is not necessary The adjustment is carried out automatically by means of the switching controller in the regulating transformer ES le 1 gt Connect the power cable to the socket of the base and to the power source gt Connect the lamp The sockets accept two lamps with a maximum combined loading of 40 W 38 Leica M Stereomicroscopes Illuminations Switching on and off Changing fuses In the event of overload You can prolong the life of your bulb if you 1 Y gt First switch off the lamp by turning the rotary knob into the black range gt and only then switch the transformer on or off gt Turn on the power switch The diode is illuminated ET 6V 6 gt Use the rotary knob to adjust the brightness Above 6 V the diode is illuminated Fuses 1 0A 5x20 stock no 167 651 gt Lamp off gt Power switch off gt Use a flat bladed screwdriver to press on the slots in the fuse holders 3 and turn the fuse holders to the left Pull out the fuse holder Fit a new fuse and replace the fuse holder gt Lamp off gt Power switch off Wait a few seconds gt Power switch on gt Lamp on Step transformer
17. 5 5 5 2 s z sS 0 63 63 333 79 26 6 12 6 16 7 5 42 3 9 53 8 2 105 3 2 65 6 9 4 22 3 0 8 8 26 3 10 21 16 13 1 64 32 8 5 42 25 84 4 52 5 119 176 1 10 21 125 168 20 105 8 26 3 6 3 33 3 31 67 7 5 42 149 141 125 125 168 15 6 13 5 25 84 10 21 78 26 9 39 53 8 63 333 187 112 10x 21B 18 6 131 20 105 32 66 128 164 10 21 5 4 8 263 239 2 20 105 25 84 40 53 16 13 1 125 16 8 63 33 3 10 21 29 9 7 25 25 84 313 67 50 42 20 105 156 135 18 26 9 125 168 373 56 32 32 66 40 53 64 33 25 6 82 20 10 5 10 21 16 13 1 418 44 4 40 53 50 42 80 26 32 6 6 25 84 125 16 8 20 10 5 59 7 35 0 63 10 1 222 126 178 20 2 111 8 1 217 63 35 6 32 70 5 448 15 149 08 128 175 16 14 25 6 8 8 10 2 22 8 28 4 56 64 35 19 1 117 1 16 14 20 112 32 7 128 175 10 224 5 44 8 8 28 23 9 94 1 25 20 112 25 9 40 56 16 14 125 179 63 35 6 10 224 29 9 75 16x 14B 16 256 88 3 7 2 44 205 109 16 8 728 175 382 59 2 32 7 40 5 6 64 35 25 6 88 20 112 10 224 16 14 418 47 25 40 5 6 50 45 80 28 32 7 25 9 125 179 20 112 59 7 38 32 512 44 64 3 5 102 4 22 4 55 32 7 16 14 25 6 88 76 4 29 4 64 35 80 28 128 18 512 44 40 5 6 20 112 32 7 955 23 0 63 15 8 15 197 12 1 315 15 126 188 98 242 49 48 5 13 30 1 235 10 1 0 8 20 11 9 25 95 40 59 16 143 125 19 63 377 10 238 29 9 79 1 25 95 313 15 50 48 20 119 156 152 78 304 125 19 373 6 4 1 25 31 3 76 39 1 6 1 62 5 38 25 95 195 122 98 242 15
18. 8 5 8 E 8 5 8 5 8 5 8 8 8 8 5 5 5 5 5 5 5 s E 3 E 3 s 3 s 5 5 5 5 e cS 0 63 63 333 79 266 126 167 5 42 39 538 2 105 32 656 94 223 0 8 8 263 10 21 16 131 64 328 5 42 25 84 4 525 119 176 1 10 21 125 168 20 105 8 263 63 333 31 677 5 42 149 141 125 125 168 156 135 25 84 10 21 78 269 39 538 63 333 187 112 16 16 131 20 105 32 66 128 164 10 21 5 42 8 283 238 88 10x 21B 2 20 105 25 84 40 53 16 131 125 168 63 333 10 21 288 7 25 25 84 313 67 50 42 20 105 156 135 78 269 125 168 373 56 32 3 66 40 53 64 33 256 82 20 105 10 21 16 131 478 44 4 40 53 50 42 80 26 32 66 25 84 125 168 20 105 597 35 5 50 42 625 34 100 21 40 53 313 67 156 135 25 84 746 28 6 60 35 75 28 120 18 48 44 375 56 188 112 30 1 89 6 23 0 63 101 222 126 178 202 81 277 63 356 32 70 5 448 15 149 08 128 175 16 14 256 88 102 22 8 28 4 56 64 35 191 117 1 16 14 20 112 32 7 128 175 10 224 5 448 8 28 239 94 1 25 20 112 25 9 40 56 16 14 125 179 63 356 10 224 289 75 16 256 88 32 1 512 44 205 109 16 14 8 28 128 175 382 59 16x 14B 2 3 7 4 56 64 35 256 88 20 12 100 24 16 48 47 25 40 56 50 45 80 28 32 1 25 9 125
19. Follow the safety instructions given on page 7 You can attach a 6 V 10 W lamp to the step transformer gt Set the voltage selector on the rear side to 115 V or 230 V gt Attach the power cable to the power supply gt Connect the lamp gt Adjust the brightness with the three step switch 4 5 6 V Leica M Stereomicroscopes Illuminations 39 Assembly Lampholder clamp for side faced columns Lampholder cast foot Lampholder objective Lampholder inclinable focusing drive Lamp housing 3 lampholder arm 40 6 V 10 W incident lamp The 6 V 10 W lamp is an inclined incident source for illuminating spatial objects Byusing two lamps you can soften or eliminate shadows Atthe nominal voltage 6 V the color temperature is 2700 K gt Release the clamping screw gt Unfold the lampholder arm gt Retighten the clamping screw gt Use the screw to secure the clamp to the side faced column either above or below the focusing drive gt Use the connector to attach the lamp holder arm to the clamp on either the right or the left gt Fit the 25 mm adapter to the cast foot gt Using the connector attach the lampholder to the adapter gt Remove the objective gt Slide the adapter over the objective gt Using the connector attach the lampholder to the adapter gt Unscrew a connector from the lampholder gt Release the clamping screw gt Using the thr
20. MZ6 MZ7s MZ9s and for MZ125 MZ16 and MZ16 A see p 18 The microscope carrier AX can only be fitted in position 1 to the focusing drive Optics carrier Gently lower the optics carrier into the microscope carrier gt Use the screw to secure the optics carrier in the position required Only for MZ7s gt Remove the screws Remove the rotary knobs gt Apply the adhesive scales Replace the rotary knobs Leica M Stereomicroscopes Assembly Eyepiece Objective AN Binocular tube Release the clamping screw f you have an accessory such as an ergo module page 14 a coaxial illumi nator page 47 or a video phototube page 52 fit it now to the optics carrier Tighten the clamping screw gt Tilt the binocular tube and insert the edge of its dovetail ring beneath the two lugs on the optics carrier Move the binocular tube from side to side until the locating screw on the optics carrier engages the guide gt Retighten the clamping screw Optical accessories gt Insert a graticule if necessary page 56 gt Release the clamping screws on the eye pieces gt Insert the eyepieces fully into the eye piece tubes Check that they have seated correctly gt Tighten the clamping screw You can extend the overall magnification range by using 10x 16x 25x and 40x wide eyepieces for persons wearing glasses Hold the objectives during assembly and
21. choice provider of innovative solutions to our customers needs for vision measurement lithography and analysis of microstructures Leica the leading brand for microscopes and scientific instruments developed from five brand names all with a long tradition Wild Leitz Reichert Jung and Cambridge Instruments Yet Leica symbolizes innovation as well as tradition Leica Microsystems an international company with a strong network of customer services Australia Gladesville NSW Tel 1 800 625 286 Fax 61 2 9817 8358 Austria Vienna Tel 43 1 486 80500 Fax 43 1 486 80 50 30 Canada Richmond Hill Ontario Tel 1 905 762 2000 Fax 1 905 762 89 37 China Hong Kong Tel 8522 564 6699 Fax 8522 564 4163 Denmark Herlev Tel 45 44 5401 01 Fax 445 44 5401 11 France Rueil Malmaison C dex Tel 33 1 4732 8585 Fax 33 1 4732 8586 Germany Bensheim Tel 49 6251 1360 Fax 449 6251 136 155 Italy Milan Tel 39 02 57 486 1 Fax 39 02 5740 3273 Japan Tokyo Tel 81 3 543 596 09 Fax 81 3 543 596 15 Korea Seoul Tel 82 2 514 6543 Fax 82 2 514 6548 Netherlands Rijswijk Tel 31 70 41 32 130 Fax 31 70 41 32 109 Portugal Lisbon Tel 35 1 213 814 766 Fax 435 1 213 854 668 Singapore Tel 65 6 77 97 823 Fax 65 6 77 30 628 Spain Barcelona Tel 34 93 494 9530 Fax 34 93 494 9532 Sweden Sollentuna Tel 46 8 625 45 45 Fax 46 8 625 45 10 Switzerland Glattbrugg Tel 41 44 809 3434 Fax 41 44 809 34 44 United Kingdom Milton Keynes Tel
22. disassembly to pre vent them from falling on the stage plate This applies particu larly to the 2x planapochromatic objective which is very heavy Remove the specimens from the stage plate Screw on the selected objective counter clockwise Some instrument configurations require spacer rings in order to secure the objectives page 32 These rings are fitted and removed using the tool provided The Leica MZ9s is supplied with a spacer ring for the achro matic objectives and for the 1x planachromatic objective When the spacer ring has been removed by applying the tool provided the larger diameter planachromatic and planapo chromatic objectives for the MZ125 MZ16 can be used Leica M Stereomicroscopes Assembly 35 36 i Preparation Assembling 1x planapochromatic Objective nosepiece e Suggested for Leica MZ16 and MZ16 A Please note When using the objective nosepiece together with MS5 MZ6 755 295 or MZ12s large fields of view at low magnifications are not fully illuminated Hold objectives during assembly and disassembly to prevent them from falling on the stage plate This applies particularly to the 2x planapochro matic objective which is very heavy Remove the specimens from the stage plate The adjustable ring nut with the 1x planapochromatic objective is fastened with 2 Allen screws after the adjustment Loosen the Allen screws if you need to remove the objective
23. from 10 50 so gt Holding the eyepiece tubes at their bases swing them up or down see arrow Leica M Stereomicroscopes Operation ErgoModule 1 Ergo objective Motor focus Interpupillary distance Locating the exit pupil ErgoWedge 5 25 ErgoWedge 15 ErgoModule 50 mm The ErgoTube 45 has eyepiece tubes which are 65 mm longer than the standard ones Magnification factor 1 6x MZ16 A The magnification factor can be toggled on and off using the OPT key ErgoModule 30 120 mm Relubrication may be required after several years please send the item to Leica Service The achromatic Ergo objective 0 4x 0 63x can be used to ergonomically and accurately focus in the range of 90 mm 63 5 153 5 mm without changing the viewing height At the same time magnification and working distance can be changed without time consuming change of objectives Frequently repeated fine motor tasks such as focusing by hand can lead to muscle fatigue and hand problems Using the Leica motor focusing system every equipment can be moved up and down without applying force Interpupillary distance eye contact The interpupillary distance is correct when you see a single circular field when viewing with both eyes gt Look into the eyepieces and use both hands to move the eyepiece tubes closer together or further apart The distance between eye and eyepiec
24. functions of the Leica MZ16 A are shown in the user manual M2 116 0 Leica MZ6 10 Leica M Stereomicroscopes Overview Leica 775 Leica MZ95 Leica design by Ernest Igl Christophe Apoth loz Leica MZ125 Leica MZ16 Leica M Stereomicroscopes Overview 11 Use Changing the magnification Ratchet steps Magnification changer Zoom means continuous change of magnification Ratchets can be engaged for certain magnifications see below Forzooming with MZ16 A see the user manual M2 116 0 55 5 steps 26 6 1 zoom MZ7s 7 9 1 zoom 295 9 5 1 zoom MZ12s 12 5 1 zoom MZ16 and MZ16 A 16 1 zoom The magnification changer can be operated from either the right or the left side gt Look into the eyepieces gt Focus on the object page 16 p Turn the magnification changer to the desired position MZ6 0 8 1 1 25 1 6 2 2 5 3 2 275 0 8 1 1 25 1 6 2 2 5 3 2 4 295 0 8 1 1 25 1 6 2 2 5 3 2 4 5 2125 1 1 25 1 6 2 2 5 3 2 4 5 6 3 8 MZ16 0 8 1 1 25 1 6 2 2 5 3 2 4 5 6 3 8 10 Engage the ratchets whenever magnifications need to be exactly reproducible p For MZ6 795 MZ12s and MZ16 Press the switch p For MZ7s Turn the knurled ring counterclockwise n For setting ratchets at the MZ16 A E see the user manual M2 116 0 Leica M Stereomicroscopes Operation Scale of magnifications Magnifications and field
25. table Hold column against the column seat from underneath gt Insert the Allen screws into the column from the top and tighten them gt Invert the transmitted light base and insert the glass stage plate gt Fit additional components such as the microscope carrier and optics carrier page 34 Add a very luminous cold light source and a light guide with a channel to the transmitted light stand TVC active 9 mm gt Insert light guide into the base at the rear gt Connect light guide to the light source For stereomicroscopes with high apertures such as Leica MZ125 MZ16 and MZ16 A with objective 1 6x the resolution can be increased by inserting an accessory condenser gt Push the accessory condenser into the lens holder at the underside of the transmitted light base and secure it with 2 Allen screws gt Turn on the light source according to the manufacturer s user manual and adjust the light intensity gt Place a transparent object on the glass stage plate and focus The built in mirror features a clear and an obscured side and can be rotated and moved The obscured side provides a dif fused illumination The angle of incidence in the specimen plane changes depending on the position of the mirror so that the transmitted light bright field via oblique illumination can also generate an illumination similar to dark field gt Rotate and move the mirror with the black rotary knob on the right side gt
26. the eyepiece Align the gratic ule by turning the eyepiece in the tube and tighten the clamping screw A detailed description can be found in the user manual 2 275 Leica M Stereomicroscopes Accessories Gliding stage Cup stage Thermo stages Stages The 120 mm stages can be used in those incident and trans mitted light stands which have a 120 mm port The gliding stage can be moved in all directions and rotated completely gt Place the 120 mm gliding stage in the 120 mm port and secure it with the eccentric lever gt Insert the 120 mm glass stage plate or the black white stage plate The cup stage can be inclined as required You can secure objects to its rubberized upper surface with needles The movable holder accepts petri dishes Place the 120 mm support ring in the 120 mm port p Place the cup stage on it X The Leica MATS thermocontrol system is a heating system for microscopes and stereomicroscopes Leica MATS allows the observation of temperature sensitive specimens and living cells in biology medicine and pharmaceutics under exact tempera ture conditions For details see M2 227 0 instructions for use Polarization transmitted light The analyzer in combination with either the rotatable polariza tion stage or the polarizer on glass stage plate enables double refracting materials to be studied These can include organic
27. the lamp and release the clamping screw gt Point the lamp vertically downwards at a white sheet of paper so that two filament images are seen gt Retighten the clamping screw gt Using the slotted screw bring the two filament images into focus gt Displace the filament images so that they are opposite to one another but in contact gt Swing back the diffusing filter Leica M Stereomicroscopes Illuminations i Adapter gt 300 mm focusing drive Adapter gt baseplate Leica L2 cold light source The antistatic Leica L2 cold light source is powerful small compact inexpensive and suitable for simple observation tasks with the Leica MS5 and MZ6 stereo microscopes In addition to one or two arm light guides corresponding accessories are al so available for the coaxial vertical and transmitted light illumi nation methods The Leica L2 can be directly connected on the stand The light guides can be mounted with arms in the threads on the microscope carrier For instructions on mounting the clamp for fiber optic light guides see page 46 For detailed information refer to the separate user manual Leica L2 M2 288 0 When in use the Leica L2 fiber optic light source must always be locked into an adapter to ensure stability Secure the clamp to the 300 mm focusing drive by tightening the screw gt Using the mounting rail slide the fiber optic light source into the adapter fork until i
28. 096 light is available Trinocular video phototube 50 gt Fit the trinocular video phototube on the optics carrier in place of the binocular observation tube page 35 For examples on camera assembly see page 54 5096 to both eyepieces 5096 in the video photo beam path Before taking the photograph close off the right eyepiece to keep out stray light gt Move lever to the left Leica M Stereomicroscopes Accessories 53 Switchover Light partition setting VIS Light partition setting PHOT Camera setup variants Trinocular video phototube 100 Fitting See Trinocular video phototube 50 Observation Push lever in gt Photography Pull out lever to the left 10096 in both eyepieces e 100 in the left eyepiece e 100 in the video photo beam path As shown in the setup diagram on page 52 there are various possibilities for adapting an MPS video digital or SLR camera Example With this equipment you can for example attach an MPS camera see detailed description in the MPS user manual an SLR camera or even a digital or video camera gt Fit the 1x video photo objective i With this equipment you can for example attach a digital or video camera gt Fit the video objective 2 on a Leica DC digital camera 3 with the C mount connection gt Connect the video objective with the mounted Leica DC digital camera on the right output of the video photot
29. 184 16 255 93 165 144 25x 9 5B 2 318 75 206 115 25 398 6 257 92 32 51 47 329 72 4 637 37 412 58 5 796 3 514 46 6 955 25 617 38 0 63 16 149 104 23 08 204 118 132 182 1 255 94 165 145 125 318 75 206 117 16 408 59 263 9 40x 6B 2 51 47 329 13 25 637 38 412 58 32 815 29 527 46 4 101 9 24 658 36 5 1274 19 823 29 6 1529 16 988 24 Leica M Stereomicroscopes User Manual 65 Optical data of Leica MZ125 MZ16 MZ16 A 1x Plan 0 5x Plan 0 63x Planapo 0 8x Plan 1 6x Planapo 2x Planapo Objectives 1x Planapo 5 Working distance mm 60 135 97 112 Plan 19 15 Planapo 55 Planapo s Eyepieces 5 5 5 E 5 5 Ss e e ie e e e eS S E 5 5 5 5 2 5 2 5 5 5 5 5 5 E 5 3 3 0 71 7 29 6 35 59 1 45 47 57 37 114 18 5 142 148 08 8 26 3 4 52 5 5 1 41 2 64 32 8 128 164 16 13 1 1 10 21 5 42 6 4 32 8 8 26 3 16 13 1 20 10 5 125 125 16 8 6 3 333 8 26 3 10 21 20 10 5 25 84 16 16 13 1 8 26 3 10 2 20 6 128 164 25 6 82 32 6 6 2 20 10 5 10 21 128 164 16 13 1 32 6 6 40 53 10x 21B 25 25 84 125 168 16 131 20 105 40 53 50 42 32 32 6 6 16 13 1 20 5 102 25 6 82 512 41 64 33 4 40 53 20 10 5 25
30. 20 W Operating environmental conditions Ambient temperature Relative humidity Atmospheric pressure 10 35 C Ambient temperature up to 35 75 700 1060 hPa Transport and storage Temperature Relative humidity Atmospheric pressure 20 52 C 10 95 condensing 500 1200 hPa Protection class Class Il Symbols Insulation Protection class Il Safety transformer protection class SEV mark of conformity reference number OG 2 Regulating transformer Technichal data Model code Transformer MTR38 Outlet 2x Connection for an external incident illuminator up to 20 W Operating voltage Primary 100 240 VAC 47 63Hz 1 8 A Secondary 5 3 V DC 7 5V 40 VA Primary fuse 2x T1AL 250 V Ambient conditions during operation Ambient temperatures 10 35 Rel humidity Up to 35 C ambient temperature 75 Atmospheric pressure 700 1060 hPa Transport and storage Temperature Rel humidity Atmospheric pressure 20 52 10 95 non condensing 500 1200 hPa Protection class 30 according to EN 60529 529 Symbols 74 Leica M Stereomicroscopes User Manual Leica Microsystems the brand for outstanding products Leica Microsystems mission is to be the world s first
31. 44 1908 246 246 Fax 44 1908 609 992 USA Bannockburn Illinois Tel 1 800 248 0123 Fax 1 847 405 0164 and representatives of Leica Microsystems in more than 100 countries In accordance with the ISO 9001 certificate Leica Microsystems Switzerland Ltd Business Unit Stereo amp Macroscope Systems has at its disposal a management system that meets the requirements of the international standard for quality management In addition production meets the requirements ofthe international standard ISO 14001 for environmental management Telephone 41 71 726 33 33 Fax 41 71 726 33 99 www leica microsystems com www stereomicroscopy com Leica Microsystems Switzerland Ltd Stereo amp Macroscope Systems CH 9435 Heerbrugg The companies of the Leica Microsystems Group operate internationally in four business segments where we rank with the market leaders Microscopy Systems Our expertise in microscopy is the basis for all our solutions for visualization measurement andanalysis of microstructures in life sciences and industry With confocal laser technology and image analysis systems we provide three dimensional viewing facilities and offer new solutions for cytogenetics pathology and mate rials sciences Specimen Preparation We provide comprehensive systems and ser vices for clinical histo and cytopathology applications biomedical research and indus trial quality assurance Our product range includes instruments sys
32. 6 152 46 6 5 1 25 8 5 16 40 59 50 48 80 3 32 74 25 95 125 19 20 119 597 4 2 50 48 62 5 3 8 100 24 40 59 31 3 76 156 152 25 95 746 32 25 62 5 3 8 78 1 3 125 1 9 50 48 39 1 6 1 19 5 122 313 76 93 3 25 32 80 3 100 24 160 15 64 37 50 43 25 95 40 59 1194 2 4 100 24 125 19 200 12 80 3 62 5 3 8 313 76 50 48 1493 16 0 63 252 95 315 15 504 48 202 11 9 158 152 79 304 126 19 37 6 6 4 0 8 32 7 5 40 6 64 38 25 6 94 20 12 10 24 16 15 418 5 1 40 6 50 48 80 3 32 75 25 9 6 125 192 20 12 59 7 4 125 50 48 62 5 38 100 24 40 6 31 3 LI 15 6 154 25 9 6 746 32 40x 6B 16 6 38 1 19 5 2 47 40 6 2 75 95 25 2 80 3 100 24 160 15 64 38 50 48 25 9 6 40 6 1194 2 25 100 24 125 19 200 12 80 3 62 5 38 313 71 50 48 1493 16 32 128 19 160 15 256 0 9 1024 23 80 3 40 6 64 38 191 13 4 160 15 200 12 320 08 128 19 100 24 50 48 80 3 238 8 1 MS5 Positions 0 63 1 1 6 2 5 4 When using the planachromatic and planapochromatic objectives MZ125 the magnification is increased by the factor 1 25x 62 Leica M Stereomicroscopes User Manual Ergo objective Objectives 0 4 0 63 Working distance mm 63 5 mm 153 5 mm e e FS Eyepieces 8 5 5 E E 3 S 2 8 8 2 8 5 E Ej s 3 z 2 0 63 4 525 26 808 08 5 412 33 636 1 64 328 4 512 125 8 26 3 5 412 10x
33. 6 82 32 6 6 64 33 80 26 5 50 42 25 84 32 6 6 40 53 80 26 100 21 6 3 63 3 3 315 67 40 3 52 504 42 100 8 21 126 17 8 80 26 40 53 512 41 64 33 128 16 160 13 10 100 21 50 4 2 64 33 80 26 160 13 200 11 11 5 115 18 575 37 725 29 92 22 184 11 230 0 9 0 71 114 197 57 394 12 31 3 9 1 246 18 2 123 22 8 9 8 0 8 128 175 64 35 82 213 10 2 22 205 10 9 25 6 8 8 1 16 14 8 28 10 2 22 128 175 25 6 88 32 7 135 20 112 10 224 128 175 16 14 32 7 40 5 6 16 25 6 8 8 128 175 164 137 205 109 4 55 512 44 2 32 7 16 14 205 109 25 6 8 8 512 44 64 35 16x 14B 25 40 56 20 112 256 88 32 1 64 35 80 28 32 512 44 25 6 8 8 328 6 8 41 55 81 9 27 1024 22 4 64 35 32 7 41 55 512 44 1024 22 128 18 5 80 28 40 5 6 51 2 44 64 35 128 18 160 14 63 100 8 22 504 44 64 5 35 80 6 28 161 3 14 201 6 11 8 128 18 64 35 81 9 27 1024 22 204 8 11 256 0 9 10 160 14 80 28 102 4 22 128 18 256 0 9 320 0 7 115 184 12 92 24 115 9 19 1472 15 2944 0 8 368 0 6 071 177 134 8 9 26 8 11 1 21 2 142 16 7 28 3 84 354 67 0 8 20 11 9 10 23 8 128 186 16 148 32 74 40 59 1 25 95 125 19 16 148 20 119 40 59 50 48 135 313 76 15 6 15 2 20 119 25 95 50 48 62 5 38 16 40 59 20 119 25 6 93 32 74 64 37 80 3 2 50 48 25 95 32 74 40 59 80 3 100 24 25 62 5 38 313 76 40 59 50 48 100 24 125 19 25x 9 5B 3 2 80 3 40 5 9 512 46 64 37 128 13 160 15 4 100 24 50 43 64 37 80 3 160 15 200 12 5 125 13 62 5 38 80 3 100 24 200 12 250 1 63 157 5 15 78 8 3 100 8 24 126 19 252 0 9 315 0 8 8 200 12 100 24 128 19 160 15 320 07 400 0 6 10 250 1 125 19 1
34. 60 15 200 12 400 0 6 500 0 5 1115 287 5 0 8 143 8 17 181 1 13 230 1 460 05 575 04 071 284 84 142 16 9 179 134 227 105 45 4 5 3 56 8 4 2 0 8 32 15 16 15 205 117 25 6 94 512 47 64 38 1 40 6 20 12 25 6 94 32 75 64 38 80 3 1 25 50 43 25 96 32 15 40 6 80 3 100 24 16 64 38 32 15 41 59 512 47 1024 23 128 19 2 80 3 40 6 51 2 41 64 38 128 19 160 15 40x 6B 25 100 24 50 48 64 38 80 3 160 15 200 12 2 32 128 19 64 38 81 9 29 1024 23 204 8 12 256 0 9 4 160 15 80 3 1024 23 128 19 256 0 9 320 0 8 5 200 12 100 24 128 19 160 15 320 0 8 400 0 6 63 252 1 126 19 161 3 15 201 6 12 403 2 0 6 504 0 5 8 320 0 8 160 15 204 8 12 256 0 9 512 05 640 04 10 400 06 200 12 256 0 9 320 0 8 640 04 800 0 3 115 460 05 230 1 289 8 0 8 368 0 6 736 0 3 920 0 3 Zoom positions 0 71 and 11 5 only for MZ16 MZ16 A 66 Leica M Stereomicroscopes User Manual Dimensions of Leica MS5 with incident light stand 1x Achromat D 198 9 Measurements in mm 315 with transmitted light stand EIS x Achromat ld 2 ba 198 9 a MS5 d d 2 Q 5 1 Y 80 2 120 328 2 Additional dimensional diagrams invol
35. Adjust exact bright field and maximum illumination move mirror to the stand column until it stops Rotate the mirror until it engages in the 45 position Detailed information about setup operation and use can be found in the separate instructions for use M2 216 2 Leica M Stereomicroscopes Assembly 27 Assembly of column base Safety ring column Cross member gt column Horizontal arm gt cross member Drive housing horizontal arm Optics carrier gt microscope carrier 28 Swinging arm stand ESD The swinging arm stand ESD is of conducting material surface resistance lt 10 Ohm squared discharge time lt 2 seconds from 1 000 V to 100 V Two sockets for connecting 074 mm ground cables are provided on the base gt Insert 4 Allen screws into the base from beneath and secure them to the end of the column The safety ring secures the instrument and must always be fixed beneath the cross member gt Slide the safety ring down the column gt Retighten the clamping screw gt Lower the cross member onto the safety ring gt Retighten the clamping screw Slide the horizontal arm into the cross member with the connecting piece towards you gt Retighten the clamping screw The drive housing microscope carrier can be fitted to the horizontal arm in either of two positions see page 34 The circular port in the microscope carrier accepts an illuminator gt Insert th
36. al From oil and grease Do not grease guide surfaces or mechanical parts kx gt Leica M Stereomicroscopes Notes 59 Therefore Cleaning polymer components Never clean polymers this way AN You can clean polymers harmlessly AN 60 Cleaning Dust and dirt will affect the quality of your results Put a dust cover over the instrument during breaks in work gt Use dust caps to protect tube openings tubes without eyepieces and eyepieces gt Remove dust with a pneumatic rubber bulb or with a soft brush gt Clean objectives and eyepieces with special optic cleaning cloths and with pure alcohol gt When accessories are not being used protect them against dust Alkohol Some components are made of polymer or are polymer coated They are therefore pleasant and convenient to handle The use of unsuitable cleaning agents and techniques can damage polymers so always observe the following rules Never clean them in an ultrasonic bath The polymer can become brittle and later break Never use corrosive cleaning agents or ones containing acetone Never use solvents for cleaning except for ethanol and isopropanol With warm soapy water wiping down afterwards with distilled water With ethanol industrial alcohol and isopropanol When cleaning with ethanol or isopropanol adhere to the appropriate safety regulations Leica M Stereomicroscopes Notes Total mag
37. between the lamp housing and the trans former page 38 Switch on the transformer and use the rotary knob to adjust the brightness Illuminating the object gt Push the condenser lens forwards or backwards until the slider engages gt Hold the lamp housing and release the clamping screw gt Align the light spot with the object gt Retighten the clamping screw gt Adjust the size of the light spot by moving the condenser lens forward or back gt If necessary swing in a filter page 42 Leica M Stereomicroscopes Illuminations 43 44 Changing the bulb Centering the bulb Disconnect the lamp cable from the transformer Never touch the bulb when it is hot You may sustain burns Donottouch the 6 V 20 W halogen bulb directly with your fin gers because finger marks can cause itto crack when heated Remove finger marks immediately with a cloth dipped in pure alcohol Press the spring clips together and pull off the rear part of the housing gt Hold the new bulb in a cloth and push it into position Press the spring clips together and close the housing again gt Center the bulb The 6 V 20 W bulb is a powerful source of illumination The more carefully you set it up the more uniform the lighting will be For this reason recenter a new bulb after fitting it Switch on the lamp Swing out the diffusing filter Pull the condenser lens fully out gt Hold
38. crystals mineral grains thin sections of rock or bone polymers stressed glass and liquid crystals A detailed description can be found in the user manual M2 277 Leica M Stereomicroscopes Accessories 57 Notes The field of view is partly shadowed The image does not stay in focus The focusing drifts downwards or can be moved only with difficulty If an electrical fault develops first check the following The image is too dark The image on the screen is too dark Blurred photographs Color photographs have a yellow cast 58 Tips and hints What to do Remedy Setthe interpupillary distance correctly page 15 Locate the eyepoint page 15 Remedy Fitthe eyepieces correctly page 35 Adjust your dioptric settings exactly as described page 20 Remedy e Adjust the ease of movement pages 16 29 31 sthe voltage selector set correctly bright field transmitted light stand page 24 transformer page 38 Is the power switch on Is the power cable attached correctly Are all connecting cables attached correctly Are the fuses in order Remedy Turn up the regulating knob high enough bright field transmitted light stand page 24 transformer page 38 Open the double iris diaphragm of the phototube TV photography Remedy Adjustthe video camera monitor system in accordance with the directions of the manufacturer Refer to the remed
39. e Stand Incident light stand Remove the stage plate gt Insert 3 Allen screws into the underside of the baseplate and secure them to the bottom end of the side faced column gt Insert the stage plate To fit the microscope carrier and the stereomicroscope see page 34 Transmitted light stand Pull the power cable out of the socket in the stand Remove the glass stage plate gt Carefully invert the transmitted light base and remove 7 Allen screws from the base gt Lift off the lower part of the base gt Reverse the upper part of the base gt Insert 3 Allen screws into the cover plate from beneath and secure them to the bottom end of the side faced column gt Replace and secure the base gt Insert the glass stage plate gt Fit the microscope carrier and the stereomicroscope as described on page 34 Technical data see page 74 Leica M Stereomicroscopes Assembly 23 24 Fitting the bulb Filters Starting up Transmitted light stand bright field 20 W Follow the safety instructions given on page 7 gt Fit the side faced column to the transmitted light base as described on page 23 Never touch the bulb when it is hot You may sustain burns e Do nottouch the new 6 V 20 W halogen bulb directly with your fingers because finger marks can cause itto crack when heated Remove finger marks immediately with a cloth dipped in pure alcohol gt Insert the slider to
40. e measures approx 22 mm for 10x 21B wide field eyepieces for persons wearing glasses and 12 mm for 10x 21 wide field eyepieces gt Slowly approach the eyepieces with your eyes until you can see the complete image field without corner cutting Leica M Stereomicroscopes Operation 15 Selecting the degree of eye contact Soft eyecups You do not wear glasses and prefer close contact with 10x 21B wide field eyepieces for persons wearing glasses gt Holding the diopter ring turn the eyecup counterclockwise until it is released p Pull the eyecup upwards gt Holding the diopter ring turn the eyecup clockwise Persons wearing glasses need the whole of the available distance Move the eyecups to the lowest position The 10x 21B wide field eyepieces for persons wearing glasses are supplied with soft eyecups which can be attached to the in tegrated eyecups made of hard plastic to achieve the following Prevent eye infections if several users are working at the same instrument see p 7 Protect your glasses from being scratched The 10x 21 wide field eyepieces are supplied with laterally tapered soft eyecups Setting the working distance focusing obtaining a sharp image To focus use the focusing drive to raise or lower the stere omicroscope until the feature of interest is in focus working distance to the objective The working distances of the various objectives are listed in the tables
41. e new bulb in a cloth and push it into position gt Replace the socket in the housing The socket clicks into position Leica M Stereomicroscopes Illuminations 4 42 Assembly Diffusing and other filters 6 V 20 W incident lamp The 6 V 20 W lamp is an inclined incident source for illuminating spatial objects Byusing two lamps you can soften or eliminate shadows Atthe nominal voltage 6 V the color temperature is 3200 K ideal for photography Set the regulating transformer to this nominal voltage see page 38 Release the clamping screw gt Unfold the lampholder arm gt Secure the lampholder to the lamp gt Use the screw to secure the clamp to the side faced column either above or below the focusing drive gt Using a fork wrench and the thread con nector attach the lampholder arm to the clamp on either the right or the left gt Position the lamp gt Retighten the clamping screw The thread connector also enables the lampholder to be connected to the cast foot e A total of 4 filter holders with 32 mm filters can be attached A heat absorbing filter is built in gt Push the filter holder complete with diffusing filter onto one of the pegs and swing it in Leica M Stereomicroscopes Illuminations Operating the lamp Observe the safety notes on page 7 AN Observe the notes with respect to the use of the transformer on page 38 gt Connect the cable
42. e peg of the inclinable focusing drive into the connecting piece gt Retighten the clamping screw p Fit the optics carrier in the microscope carrier gt Turn the optics carrier sideways in the microscope carrier as required gt Retighten the clamping screw Leica M Stereomicroscopes Assembly Focusing Tilting Altering the working distance Sideways movement Altering the overhang The focusing knobs are located on the inclinable drive housing Is the focus movement too loose or too tight Does the outfit tend to slide downwards You can adjust the movement in accordance with the weight of the outfit and with your own personal requirements gt Hold the drive knobs firmly with both hands and turn them in opposite direc tions until you obtain the required degree of resistance during focusing Possible adjustments gt Release the clamping screw gt Tilt the instrument gt Retighten the clamping screw gt Release the clamping screw gt Raise or lower the cross member as re quired together with the horizontal arm gt Retighten the clamping screw Reposition the safety ring see page 28 gt Release the clamping screw gt Move the instrument sideways on the cross member gt Retighten the clamping screw gt Release the clamping screw gt Displace the horizontal arm gt Retighten the clamping screw Leica M Stereomicroscopes Assembly h
43. ead secure the lampholder to the focusing drive gt Retighten the clamping screw gt Attach the lamp to the connector on the lampholder arm gt Fit the filter holder complete with KG1 heat absorbing filter to one of the pegs e A total of 4 filter holders with 50 mm filters can be attached Leica M Stereomicroscopes Illuminations Operating the lamp Changing the bulb Observe the safety notes on page 7 A Observe the notes with respect to the use of the trans former on page 38 gt Connect the cable between the lamp housing and the transformer page 38 Switch on the transformer and use the rotary knob to adjust the brightness page 39 gt Hold the lamp housing and release the clamping screw gt Align the light spot with the object gt Retighten the clamping screw Swing in the heat absorbing filter gt Enlarge or reduce the size of the light spot by turning the socket of the bulb Disconnect the lamp cable from the transformer Never touch the bulb when it is hot You may sustain burns You can separate the lamp housing and the bulb socket Line up the arrow head on the mount with its shaft on the housing Do not touch the 6 10 W halogen bulb because finger marks can cause itto crack when heated Remove finger marks with a cloth dipped in alcohol p Turn the socket to the separation position gt Pull the socket forcibly out of the housing Hold th
44. ective nosepiece MZ16 and MZ16 A 36 4 Leica M Stereomicroscopes User Manual Page Illuminators Transformers era libido ile 38 Incident lamp 6 10 40 Incident lamp 6 20 42 Cold light sources 45 Fiber optic light guide 46 LED illuminatio siria 46 Supply line lamp 25 W 47 Coaxial illuminator 47 Near vertical illuminator 48 Accessories Fitting 50 Double iris diaphragm 50 Drawing tube discussion tube 51 Video phototubes Photography video TV filming digital imaging 52 Attachment for vertical and oblique observation 56 Graticules e De Le Enk ids 56 Stages polarization 57 Special notes Tips and hints What to 1 58 Care and maintenance 59 Calculating total magnification and field of view diameter 61 Optical data for MS5 MZ6 62 Optical data for MZ75 295 64 Optical data for MZ125 MZ16
45. ensional diagrams involving Leica ErgoModule are available from your Leica agency 70 Leica M Stereomicroscopes User Manual Dimensions of Leica MZ12 5 with incident light stand 1x Plan HH LAU Ut i 222 Measurements in mm TT 1 2125 00 DI Y fei a 1 C Y Y 7 120 5 285 320 with transmitted light stand 267 138 385 216 2 1x P 447 290 8 325 Additional dimensional diagrams involving Leica ErgoModule are available from your Leica agency Leica M Stereomicroscopes User Manual 7 Dimensions of Leica MZ16 with incident light stand with transmitted light stand Im 1 Planapo
46. ereomicroscopes Accessories Drawing Drawing tube The drawing tube is for both left handers and right handers Fit the mirror on the left hand or right hand side of the optics carrier gt Fit the drawing tube between optics car rier and binocular tube as described on page 50 gt Place a sheet of paper beneath the mirror gt Use a table lamp to illuminate the paper gt Move the lever to the circle Look into the binocular tube position the object and focus on it Move the lever to the black spot gt Look into the binocular tube and balance the brightness so that the object the drawing surface and the pencil are all seen together n general the paper needs to be illuminated strongly but the object only weakly gt Move the lens mount of the drawing tube forwards or back wards until the drawing surface is sharply imaged Now you can draw the feature f you place a graduated scale beneath the object and draw its intervals as well you can easily determine the image scale on the paper Discussion tube At the discussion tube two observers see the same erect laterally correct three dimensional image The assembly and use of the discussion tube are described in the separate user manual M2 263 0 Leica M Stereomicroscopes Accessories 51 Video phototubes 1 2 3 1 2 1 3 SLR Leica B Mount B Mount C Mount C Mount CCD cameras camera housing MPS30 MPS60 3 2 3
47. ertical illuminator 10 445 198 C C CA 2x 10 446 300 CA 10 446 300 CA 10 446 300 CA 10 446 300 10 446 393 10 446 393 Attachment for vertical and 10 445 156 C C CA 2x 10 446 300 CA 10 446 300 CA 10 446 300 CA 10 446 300 oblique observation 10 446 393 10 446 393 Objective turret for 2x and 1x 10 447 107 C C 0 0 0 C C Compatible CA Compatible if used with intermediate ring order separately 0 Intermediate ring is recommended M Magnification increased by factor 1 25x or higher Remove intermediate ring 10 446 393 already included with MZ95 C Large fields of view at low magnifications are not fully illuminated Leica M Stereomicroscopes User Manual 33 Microscope carrier 3 drive housing Microscope carrier AX 34 Optics carrier 3 microscope carrier Apply the adhesive scale of magnifications Microscope carrier The microscope carrier can be attached to the focusing drive in 2 positions For short working distances and for flat objects use Position 1 Use this position also in conjunction with the attachment for vertical and oblique observation see detailed in structions Use Position 2 for long working distances and for bulky objects gt Use an Allen screw to secure the micro scope carrier in the position required A connection socket for a 4mm grounding cable is provided on the microscope carrier Different models are available for MS5
48. g dioptric settings D Inspecting parfocality 4 Engage the highest magnification position 5 Optimize the focus using the focusing drive 6 Engage the lowest magnification position 7 Do not look into the eyepieces p Rotate the eyepieces counterclockwise in the direction as far as the stop 5 diopters 8 Look into the eyepieces Slowly rotate each eyepiece individually in the direction until each eye sees the object sharply imaged 9 Engage the highest magnification position 10 Refocus if necessary 11 Slowly move the magnification changer from the lowest magnification to the highest e image sharpness must remain constant parfocal Otherwise repeat the procedure Leica M Stereomicroscopes Operation 21 Assembly 22 The components 1a 1b 2a 2b 3 A Incident light base with stage plate Transmitted light base with glass stage plate Side faced column with drive housing coarse fine Side faced column with drive housing 300 mm or 500 mm Microscope carrier Optics carrier Interchangeable objective Binocular tube Wide field eyepiece for spectacle wearers with eyecups Various ErgoModules or accessories for video photography drawing fluorescence coaxial incident light etc Leica M Stereomicroscopes Assembly Side faced column gt incident light base Side faced column gt transmitted light bas
49. gether with the bulb socket gt Hold the new bulb in a cloth and push it into position Push the slider carefully but complete ly back along the rail to the stop The transmitted light stand contains a KG1 filter You can insert other Z 50 mm filters if you need them gt Pull out the slider gt Insert the 50 mm filter gt Push the slider carefully but completely back along the rail to the stop The ex factory setting of the voltage selector is 230 for volt ages between 200 240 V and the fuse holder contains two 160 mAT fuses For 100 120 V power supply 4 gt TL Setthe voltage selector to 115 gt Fit the enclosed two 315 mAt fuses 2 in the fuse holder instead of the two 160 fuses see the section Changing fuses on the right gt Connect the power cable to the socket of the base and to the power source Leica M Stereomicroscopes Assembly Changing fuses Transmitted or incident light Switching on the transformer Disconnection from mains power supply Transmitted light Transmitted light bright field Inclined transmitted light Use Two 160 mAT fuses for voltages between 200 240 V Two 315 mAT fuses for voltages between 100 120 V see the section Starting up on the left gt Disconnect the power cable from the stand gt Pull out the fuse holder gt Fuse at rear Press out downwards Spare fuse at front Pull out s
50. harge of instrument A Repairs may only be carried out by Leica trained service tech nicians Only original Leica spare parts may be used Ensure that personnel who use the instrument have read and understood this user manual and in particular the safety instructions Ensure that the Leica MS5 MZ6 MZ7s MZ9s MZ12s MZ16 and MZ16 A stereomicroscopes are operated maintained and repaired only by authorized and trained personnel Workplaces with stereomicroscopes facilitate and improve the viewing task but they also impose high demands on the eyes and holding muscles of the user Dependent upon the duration of uninterrupted activity this may lead to asthenic trouble and muscular skeletal pain so that suitable measures must be taken to reduce the strain perfect design of workplace see p 14 work content and work process frequently changing activities detailed introduction of the personnel while considering ergonomic and organizational work related aspects The ergonomic optics concept and the design of the Leica M series aim at limiting the strain on the user to the lowest possi ble level Direct contact with eyepieces can be a potential transmission path for bacterial and viral infections of the eye Users should be made aware of the potential risk of infection The risk can be kept at a minimum by using individual eyepieces or attachable eyecups see p 16 Leica M Stereomicroscopes Safety regulations Liquids
51. icroscopes Leica MZ16 A and MZ16 FA can be found in separate user manuals Leica M Stereomicroscopes User Manual Table of contents Page Overview Safety ERES R EY A 6 Nu Pm 9 Controls and functions 10 Use Changing the 12 14 Interpupillary distance 15 15 Eye contact eyecups 16 FOCUSING dade eR Re UPS CU rre er e 16 Microscope carrier Stereoscopic and axial observation 18 Diopter Settings xoa n a ins 20 Assembly Overview Components 22 Stand Profile column for incident light and transmitted light stands 23 Transmitted light stand bright field 20W 23 Transmitted light stand bright and dark field 26 Transmitted light stand HL 27 Swinging arm stand ESD 28 Large swinging arm and table clamp stands 30 Optics carrier optical accessories Spacer rings objective combinations 32 Microscope carrier 34 Optics carrier 34 Binocular tubes optical 35 Obj
52. ideways and insert into holder at rear d gt Push the fuse holder back into position On the rear side of the base there is a socket for incident lamps of up to 20 W Connectan incident lamp to the trans mitted light stand if required Either transmitted light or incident light can be switched on gt For transmitted light Switch in position p For incident light Switch in position Il gt Use thumb wheel to adjust brightness gt On the rear of the stand Use the thumb wheel to switch on the transformer and to adjust the brightness 1 Remove the power cable from the power socket Switch on transmitted light Bright field is suitable for examining translucent objects featur ing contrasting structures The objectis directly illuminated from below and is seen in its natural colors against a bright background gt For transmitted light bright field Pull lever forwards Inclined transmitted light which traverses the object obliquely will produce effects advantageous for observing small translu cent objects such as foraminifera and fish eggs Move the lever slowly towards the column of the stand until the desired effect is obtained Leica M Stereomicroscopes Assembly 25 Starting up Transmitted light bright field Transmitted light dark field Transmitted light stand bright and dark field The illumination of the transmitted light stand bright and dark
53. ies listed above under The image is too dark Remedy Focus precisely page 16 Adjust your dioptric settings exactly as described page 20 Remedy Use artificial light film Turn the regulating knob of the transformer clockwise to the position of maximum voltage bright field transmitted light stand page 24 transformer page 38 Leica M Stereomicroscopes Notes In this section We guarantee the quality Protect your instruments Care maintenance We will show you how to look after your microscope and how to clean it You are working with a high precision high performance instrument Accordingly we guarantee the quality of each of our instru ments The guarantee covers all faults in materials and manu facture It does not however cover damage resulting from careless or improper handling Please handle your valuable optical instrument with care It will then fulfill its function with the same precision for decades Our instruments are known for that If contrary to expectations your stereomi croscope develops a fault please call in a specialist contact your local Leica agent or Leica Microsystems Switzerland Ltd CH 9435 Heerbrugg From moisture vapors acids alkalis and corrosive substances and keep chemicals away from the instruments From improper handling Never dismantle optical systems or mechanical parts unless instructions for doing so are given in the user manu
54. ion The attachment for vertical and oblique observation enables spatial objects to be observed from above and also from all around at an angle of 45 The microscope carrier must be fitted in the lower position 1 page 34 For MS5 MZ6 Fit the 1x achromatic objective by screwing its adapter ring directly into the optics carrier Spacer rings are required for the Leica MZ75 295 MZ12s and MZ16 to secure the attachment for vertical and oblique observation to the optics carrier page 32 Only usable with 1x achromatic objec tive A detailed description can be found in the user manual M2 266 Graticules The graticules for length measurements and numbering are fitted in mounts and can be inserted into the eyepieces Aspecial measuring graticule is available for the Leica MZ16 A see the user manual M2 116 e A high precision object micrometer with 50 mm scale 0 1 mm and 0 01 mm graduation allows for calibration corresponding to the selected magnification Preferably measure with the microscope carrier AX in vertical position The measurements are more accurate with outthe convergence angle in the stereoscopic image Graticules show boundary lines for all film formats gt Unscrew the insert at the knurled ring N Clamp the graticule onto the insert with 7 a little bit of pressure Check for correct seating Tighten the insert with graticule C gt Insert the eyepiece gt Look into
55. is avail able with ring illuminator and or spot and suitable for routine tasks with the Leica MS5 26 MZ7s 295 stereomicroscopes LEDs which do not develop any heat are used as illuminators The color temperature measures 5000 K daylight For details see the separate instructions for use for the LED illumination Leica M Stereomicroscopes Illuminations Supply line lamp 25W Follow the safety instructions given on page 7 gt Fit the lamp holder and lamp as described below incident light lamp 6 V 10 W gt Connect the 25 W supply line lamp to the power supply Switch on the supply line lamp gt Align the light spot with the object as described Coaxial illuminator This illuminator is for observing and photographing flat highly reflecting objects such as wafers integrated circuits and polished metal sections The coaxial incident light housing fits like an accessory tube between the optics carrier and the binocular tube or between the optics carrier and the phototube page 50 e For the MZ7s and MZS9 fit the objective preferably to the spacer ring 10 446 300 page 32 This will completely illuminate the largest field of view Aquarter wave plate is required when the microscope carrier AX is used in the vertical position gt Using the clamping screw secure the quarter wave plate to the objective mount gt Using the knurled ring turn the quarter wave plate un
56. justing diopter settings and parfocality Leica stereomicroscopes are parfocally matched This means that once you have focused on a feature in the manner de scribed taking account of diopter settings you can zoom the magnification up and down without refocusing You only need to refocus if you want to look at higher or lower planes in the object A prerequisite is the correct setting of the diopters and the parfocality 25 5 e The dioptric range is from 5 to 5 The following adjustments must be carried out only once by each user The procedures for adjusting the dioptric setting and the parfocality are somewhat different if an eyepiece contains a graticule Refer to the user manuals for the MPS modular photomicrographic systems or for measuring gt Install the lamp gt Set the interpupillary distance and if necessary the viewing height gt On the video phototube Move the lever to position VIS and open the diaphragm gt Set the double iris diaphragm to OPEN gt Adjust the eyecups gt Set both eyepieces to zero gt Set the microscope carrier AX for stereoscopic observation 1 Place a flattest object under the objective 2 Engage the lowest magnification position gt Use the coarse focusing drive to set the working distance page 16 3 Look into the eyepieces gt Use the focusing drive to bring the test object into focus Leica M Stereomicroscopes Operation C Adjustin
57. lly from the outstanding optical and ergonomic advantages of the instrument if you set it up precisely in accordance with the directions already given For fatigue free work use the ErgoModules Arrange your workplace as well as possible and pay attention to the heights of chair and table e When sitting use the entire seat area and also the back support of the chair Support your forearms During work breaks stand up and move around for exercise Please also observe the note on p 7 The reasons why users often cannot work comfortably with the 45 tube are the differing heights of the outfits the various accessories and working distances and the fact that the same instrument is often used by several people of differing builds This problem can be effectively solved by using the Leica ErgoModules If you have problems with the viewing configuration on your stereomicroscope ask your Leica consultant for the most ergonomic Leica solutions With the achromatic Ergo objective 0 4x 0 63x for the Leica 55 MZ6 MZ7s and 295 it is possible to focus in the region of 90mm 63 5 mm 153 5 mm ergonomically and precisely without changing the viewing height At the same time magnification and working distance can be changed without any time consuming changing of the objectives The viewing height is correct when your head and back are comfortably positioned e Onthe ErgoTube the viewing angle is adjustable
58. nification and field of view diameter Example Calculation Mg Magnification of objective Magnification of eyepiece 7 Magnification changer position Tube factor e g 1 5x for coaxial incident light 45 1 6x r Factor 1 25x if the planachromatic and planapochro matic objectives of the MZ125 MZ16 are used on the 55 26 MZ7s or MZ95 Nfov Field number of eyepiece The field numbers are printed on the eyepieces 10x 21 16x 14 25x 9 5 40x 6 Mg Eyepiece 1x ME 25x 9 5 eyepiece 7 Zoom position 4 q Tube factor 1 5x because of coaxial illuminator Factor 1 25x Magnification down binocular tube x Mg x z x q x r 1 25 x 4 15x 125 187 5 x 2 OF Mgxzxqxr Nrov Diameter of field of view 9 5 1 3 mm 1x4x 15 x 125 Leica M Stereomicroscopes Notes 61 Optical data of Leica 55 MZ6 1x Plan 1 Planapo 1 6x Planapo 0 63x Planapo 0 5x 0 32x Achromat 0 5x Achromat 1 5x Achromat Objectives 1x Achromat 2x Achromat 0 8 Achromat 0 63 Achromat 0 8x Plan Working distance mm 5 81 Plan 55 Planapo 19 Planapo 97 Planapo 135 Plan 297 Achromat 187 Achromat 49 Achromat 89 Achromat 21 Achromat 112 Achromat 149 Achromat amp 112Plan 5 Eyepieces s 2 Pa 2 2 s s El Eb f 5153 5 o P OP E 5 5 5
59. not exceed the maximum permitted loading as this can cause damage to the equipment Before changing a bulb disconnect the power cable e Never attempt to touch the inside of a lamp housing or transmitted light stand while the instrument is connected to the power supply Before changing a bulb wait until it is cool otherwise you may burn your fingers Leica M Stereomicroscopes Safety regulations Integration in third party products Legal requirements Disposal Conformity with European Community directive A The following must observed if Leica products are build into third party products The manufacturer of the complete system or the person putting it on the market is responsible for adhering to applicable safety regulations laws and guidelines Adhere to general and local regulations relating to accident prevention and environmental protection The products described here must be disposed off in accor dance with applicable local laws and regulations The electrically operated accessories for the Leica MS5 MZ6 MZ7s MZ9s MZ12s MZ16 and MZ16 A stereomicroscopes constructed in accordance with the latest technologies and provided with a statement of conformity with EC requirements Symbols used in this user manual Safety instructions This symbol denotes information which must be read and obeyed Non observance can put personnel at risk can cause malfunctioning o
60. observation Stereoscopic observation Microscope carrier This microscope carrier permits only stereoscopic observation using both beam paths The microscope carrier can be fitted to the drive housing in either of two positions see page 34 The optics carrier can be turned sideways in the microscope carrier if the user needs to work from the side p Release the clamping screw p Turn the optics carrier sideways as required gt Retighten the clamping screw Microscope carrier AX The stereomicroscopes can be equipped with the microscope carrier AX for stereoscopic and axial observation instead of the standard microscope carrier page 34 Different models are available for MS5 MZ6 MZ7s MZ9s and for MZ12s MZ16 and MZ16 A The achromatic objectives and the planochromatic 1x fit in the thread in the AX microscope carrier for MS5 MZ6 MZ7s 295 p 33 The planochromatic and planapochromatic objectives fit in the thread in the AX microscope carrier for MZ12s MZ16 MZ16 A p 33 In addition the AX microscope carrier features a clamp ing screw for fixing it in one of 3 positions and an aperture which can be turned manually see below Otherwise the use of and switching to the left middle and right position are identical The optics carrier cannot be turned sideways in the micro scope carrier AX A three dimensional image is required for examining plastic objects and for working
61. on pages 62 66 The focusing drive can be operated from either the right or the left side When focusing on a new object always start with the lowest magnification because the larger field of view makes the object easier to find Afterwards select the desired magnifi cation Leica M Stereomicroscopes Operation Coarse fine drive Adjusting the free running of the focusing drive Focusing drive MS5 MZ9 Focusing drive coarse fine p Place the object beneath the objective gt Engage the lowest magnification page 12 gt Look into the eyepieces gt Using the rotary knob bring the object into focus The coarse fine drive supports up to 15 kg Resolution with 5 kg load 1 um Resolution with 10 kg load 2 um gt Focus approximately with the larger knob gt Fine focus with the smaller concentric knob Is the focus movement too loose or too tight Does the outfit tend to slide downwards You can adjust the movement in accordance with the weight of the outfit and with your own personal requirements gt Hold the drive knobs firmly with both hands and turn them in opposite directions until you obtain the required degree of resistance during focusing gt Hold the left or right fine drive gt Turn the other fine drive until the desired resistance during focusing is reached Leica M Stereomicroscopes Operation 17 Microscope carrier for stereoscopic
62. ovided the larger diameter planachromatic and planapochromatic objectives for the MZ125 MZ16 can be used The Leica MS5 MZ6 and MZ75 can also be equipped with the larger diameter planachromatic and planapochromatic objec tives for the MZ125 MZ16 if a spacer ring is fitted 10 446 172 When the MZ125 MZ16 planachromatic and planapo chromatic objectives are used on the Leica MS5 MZ6 MZ75 and MZ95 the magnification is increased by the factor 1 25x 10447 103 MZ16 A MZ16 10 446 370 MZ125 10 446 272 295 10 447 107 10 445 614 MZ6 10 445 613 MS5 10 445 819 1 Plan 10 411 589 1 10 446 275 10446 157 0 5x Plan 10 422 562 1 5 10 447 075 0 8x Plan 10 447 081 2x 10 447 157 1x Planapo 10 446 172 10 473 832 0 8x 10 447 101 2x Planapo t J 10 447 148 10 445 201 0 63x 10 447 050 1 6x Planapo e 0 4 0 63x 10 422 563 0 5 10 447 051 0 63x Planapo 10411589 1x Si Ergo 10 422 564 0 32x Plan Planapo 10 422 562 1 5x an Achromat 10 447 081 2x 10 446 157 0 5x Plan 10 473 832 0 8x 10 447 075 0 8x Plan 10 445 201 0 63x 10 447 157 1x Planapo 10 422 563 0 5x 10 447 101 2x Planapo 10 422 564 0 32x 10 447 050 1 6x Planapo Achromat 10 447 051 0 63x Plan Planapo gt 10367 929 10 315 306 10 445 352 gt 10 367 929 10 315 306 10 445 352 32 Leica M Stereomicroscopes Assembly
63. r damage to the equipment Warning about accessible hot places e g bulbs Failure to respect it can cause burns Important information This symbol denotes additional information or explanations which promote understanding Action p This symbol within the text indicates that certain operations must be carried out Explanatory notes e This symbol within the text stands for additional information and explanations Leica M Stereomicroscopes Safety regulations 9 Overview Stereomicroscope 1 Magnification changer 2 Engaging ratchet positions 3a Focusing drive 3b Coarse and fine focusing Larger knob Coarse focusing Smaller knob Fine focusing 3c Adjustable ring Changes the ease of movement of the coarse fine drive 4 Clamping screw Holds the optics carrier in the microscope Carrier 5 Clamping screw Holds the binocular tube or accessory to the optics carrier 6 Interchangeable objective Achromatic planachromatic or planapochromatic Leica MS5 Binocular tube 1 Adjustable eyepiece tubes Interpupillary distance adjustable from 52 76 mm 8 Wide field eyepieces for persons wearing glasses Diopter setting adjustable within the range from 5 to 5 and eyecups adjustable 9 Clamping screws These hold the eyepieces in the binocular tube 10 ErgoTube The viewing angle is adjustable within the range 10 50 11 Built in double iris diaphragm MZ16 MZ16 A only e The special
64. s of view Magnification with MZ125 MZ16 planachromatic and planapochromatic objectives MS5 MZ6 Display of magnification factors from 0 63 4 275 Display of magnification factors from 0 63 5 Corresponding scales for other objective and eyepiece combi nations can be affixed to the rotary knobs page 34 MZ9 Display of magnification factors from 0 63 6 MZ12 Display of magnification factors from 0 8 10 MZ16 MZ16 A Display of magnification factors from 0 71 11 5 MZ16 A For the digital display of the magnification see the user manual M2 116 0 The tables on pages 62 66 provide information about the magnifications and field of view diameters as a function of the magnification changer position of the eyepiece objective combination used When the MZ125 MZ16 planachromatic and planapochromatic objectives are used on the Leica MS5 MZ6 MZ75 and 295 the magnification is increased by the factor 1 25x This factor is taken into account in the tables Magnification on Magnification on MZ125 MZ16 MS5 MZ6 MZ75 MZ9s 1x plan planapo 1 25x 1 6x planapo 2x 0 63x planapo 0 8x 0 5x plan 0 63x 0 8x plan 1x 2x planapo 2 5x Leica M Stereomicroscopes Operation 13 Preconditions for working comfortably Ergonomics Ergo objective Viewing height viewing angle Ergonomics viewing height Pay great attention when setting up your stereomicroscope You will only benefit fu
65. t engages When mounted on the baseplate the light source can be used as a stand alone unit gt Using the mounting rail slide the fiber optic light source into the adapter fork until it engages Leica M Stereomicroscopes Illuminations 45 46 Fiber optic light guide Cold light source Leica CLS series The Leica CLS series is a high performance line for high light intensity within the smallest possible space and flicker free white light with the lowest possible thermal effect on the ob jects The comprehensive line of accessories enables unlimited use The CLS series is produced of antistatic material When using the ring lamp 76 mm on the planachromatic objective 0 8x 07 80 mm a special adapter 10 447 078 is required For a detailed information refer to the user manual Leica CLS The clamp ofthe fiber optic light guide cannot be attached to the microscope carrier AX gt Turn the clamping screw out of the clamp ee gt Hold the clamp in the opening of the microscope carrier gt Push the clamping screw through the holes in the microscope carrier and in the broader part of the clamp and screw it finger tight to the narrow part of the clamp gt Insert the fiber optic light guide into the clamp Switch on the power unit gt Align the light spot with the object gt Retighten the clamping screw Leica LED illumination The Leica LED1000 Laser Emitting Diode illumination
66. t resolution Screw the 2x planapochromatic objective into the beam path gt Adjusting the diopter settings and parfocality see p 20 Screw the 1x planapochromatic objective into the beam path Observe the same object with smallest magnifica tion without changing the settings made with the 2x planapochromatic objective gt Turn the 1x planapochromatic objective counter clockwise until the object can be seen in focus at the smallest magnification Slowly zoom to the highest magnification At the same time correct the focus by turning the 1x planapochromatic objective Verify that the objectis in focus at highest magnification with the 2x planapochromatic as well as the 1x planapochromatic objective without refocusing Otherwise turn the objective at the ring nut until the object is focused Fasten the ring nut with both screws Do not overtighten both screws Leica M Stereomicroscopes Assembly 37 Illuminators Transformers A Follow the safety instructions given on page 7 Leica transformers may only be opened by authorized service personnel and the power cable must be disconnected before hand The transformers are not to be used outdoors Regulating transformer The regulating transformer 5 3 to 7 5 V 40 VA is used to operate the 6 V 10 W and 6 V 20 W lamps 1 Connections for two lamps maximum combined loading 40 W 2 Connection for power cable 3 Fuse holder 2x T 1A L 250 VA
67. tems and consum ables for tissue infiltration and embedding microtomes and cryostats as well as auto mated stainers and coverslippers Medical Equipment Innovative technologies in our surgical micro scopes offer new therapeutic approaches in microsurgery Semiconductor Equipment Our automated leading edge measurement and inspection systems and our E beam lithography systems make us the first choice supplier for semiconductor manufacturers all overthe world eLca MICROSYSTEMS Illustrations descriptions and technical data are not binding and may be changed without notice M2 105 0en Leica Microsystems Switzerland Ltd CH 9435 Heerbrugg 2002 Printed in Switzerland 11 2005 RDV
68. th the enclosed wrench Screw in the 1x planapochromatic objective with the ring nut in clockwise direction on the side with the 2 Allen screws The fine pitch thread on the ring nut consists of 24 threads You should expect at least 40 turns The adjustable ring nut must never be set higher than the top surface of the nosepiece plate to prevent the nosepiece from locking while rotating Leica M Stereomicroscopes Assembly Assembling 2x planapochromatic Assembling objective nosepiece Adjusting parfocal Carefully turn and tighten the 2x planapochromatic objective clockwise to the limit stop without applying excessive force Raise the focusing drive with the drive heads 10 provide sufficient space for the 2x planapochro matic objective gt Fit the objective nosepiece like a microscope car rier with the Allen screw see page 34 position 1 gt Insert the optics carrier in the objective nosepiece gt Retighten the clamping screw gt Attach the limit stop to the column of the stand so that the working distance beneath the 2x objective will always measure approx 15 mm Fitand adjustthe 1x and 2x planapochromatic objectives as described The image then remains parfocal in the range of 0 05 mm and refocusing after changing the objective is no longer required Use a very fine flat object e g an object micrometer for the adjustment which allows maximum focusing even at highes
69. til the required effect is obtained Please observe the notes on the use of the aperture in the AX microscope carrier p 19 Leica M Stereomicroscopes Illuminations 17 For 55 MZ6 For Leica MZ7 MZ9 2125 Near vertical illuminator The near vertical approx 5 light beam illuminates depres sions and cavities Shadowing by tools is eliminated This illuminator can only be used with the achromatic objec tives having focal lengths of 100 150 175 and 200 mm Spacer rings are required for the Leica MZ75 MZ95 MZ125 to secure the near vertical illuminator to the optics carrier page 32 gt Unscrew the objective gt Release the clamping screw and remove the adapter ring from the incident light housing for near vertical light gt Screw the adapter ring onto the microscope carrier gt Secure the incident light housing to the adapter ring gt First screw the spacer ring s page 32 onto the microscope carrier then screw the adapter ring to the spacer ring gt Secure the incident light housing to the adapter ring gt Screw the achromatic objective see above into position gt Release the left or right clamping screw and change the cover plate accordingly Leica M Stereomicroscopes Illuminations No light gt Attach the fiber optic light guide on either the left or the right To switch on the cold light illuminator refer to the user manual provided with it gt
70. turn it Leica M Stereomicroscopes Assembly Microscope carrier gt Secure as described drive housing on page 34 Optics carrier gt Secure as described on page 34 microscope carrier Possible adjustments Tilting Release the clamping lever gt Tilt the instrument gt Retighten the clamping lever Focusing with Set large distances with the larger coarse fine drive knob gt Fine focus with the smaller concentric knob gt Using the ring on the right hand drive knob adjust the ease of movement of the drive Altering the working Release the clamping screw distance Raise or lower the horizontal arm gt Retighten the clamping screw Reposition the safety ring see page 30 Sideways movement Release the clamping screw gt Pull out or push in the horizontal arm gt Retighten the clamping screw During the sideways movement observe the limit stops see page 30 Altering the overhang Release the clamping screw gt Displace the horizontal arm gt Retighten the clamping screw Leica M Stereomicroscopes Assembly 31 Optics carrier optical accessories Spacerrings Spacer rings are required in order to attach the objectives for certain instrument configurations The Leica MZ95 is supplied with a spacer ring 10 446 393 for the smaller diameter achromatic objectives and 1x planachromatic objective When the spacer ring has been removed by applying the tool pr
71. ube gt Tighten the coupling ring 1 Leica M Stereomicroscopes Accessories Switchover Light partition for observation Light partition for documentation Camera setup variants Video phototube HD50 gt Use the video phototube HD50 between the optics carrier and the binocular tube page 50 gt Observation Push lever in Documentation Pull lever out 5096 to both eyepieces 5096 in the left hand video photo beam path 5096 in the right hand video photo beam path e 100 to left video photo beam path e 50 to right eyepiece e 50 in the right hand video photo beam path As shown in the setup diagram on page 52 there are various possibilities for adapting an MPS video digital or SLR camera Example gt Fit the video photo objective Honthe left hand side With this equipment you can for example attach an MPS camera see detailed description in the MPS user manual an SLR camera or even a digital or video camera Example With this equipment you can for example attach a digital or video camera p Fit the video objective 2 on a Leica DC digital camera 3 with the C mount connection gt Connect the video objective with the mounted Leica DC digital camera on the right output of the video phototube gt Tighten the coupling ring 1 Leica M Stereomicroscopes Accessories 55 56 Inserting the graticule Attachment for vertical and oblique observat
72. ving Leica ErgoModule are available from your Leica agency Leica M Stereomicroscopes User Manual 67 Dimensions of Leica 76 with incident light stand a Achromat pmi 198 9 Measurements in mm 1 MZ6 k gt Y nj i O Y m Y 120 278 with transmitted light stand 1 Achromat UJ 38 1 MZ6 5 te Ep i O 5 y 1 Y L 80 2 120 294 328 2 Additional dimensional diagrams involving Leica ErgoModule are available from your Leica agency 68 Leica M Stereomicroscopes User Manual Dimensions of Leica MZ75
73. y not be used outdoors The optics carriers MS5 26 MZ7s MZ9s MZ125 MZ16 and MZ16 A the binocular tube ErgoTube ErgoWedge 5 25 the swinging arm stand ESD and the cold light sources Leica L2 and CLS consist of ESD discharging material surface resistance 10 ohms squared discharge time 2 seconds 1 000 V to 100 V A connection socket for a 4 mm grounding cable is provided on the microscope carrier There are two connection sockets for 4 mm grounding cables in the base of the swinging arm stand The Leica M series can be used in clean rooms without any problems and they can be cleaned as described on page 60 Please observe the following rules Do not clean our stereomicroscopes and accessories in a different manner from that described in this user manual Do not use unsuitable cleaning agents chemicals and techniques Do not clean painted surfaces and accessories with rubber parts such as the ErgoTube 10 50 with chemicals This can damage the surfaces Particles or grindings can contaminate the specimen f the customer cleans our instruments with chemicals without our written approval he will do it at his own risk n most cases we can offer special solutions on request Some products can be modified or we could recommend other accessories for use in clean rooms Leica M Stereomicroscopes Safety concept Safety regulations Servicing Responsibilities of person in c

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