POCKET COLORIMETER™ II ANALYSIS SYSTEMS
Contents
1. 100 mL MDB Sulfide Inhibitor Reagent Powder Pilo ze 100 pkg Sulfuric Acid concentrated AC 500 mL Sulfuric Acid Standard Solution 1 0 N sesssssseeseses 100 mL MDB Wastewater Standard mixed parameter influent QC Ammonia Nitrate Phosphate GOD Sulfate TOC raten aaa 500 mL Wastewater Standard mixed parameter effluent QC Ammonia Nitrate Phosphate COD Sulfate LOG ET 500 mL Water AAN ici ONES RA hele ee een aie 4L eet 272 56 Ammonia Nitrogen continued Optional Apparatus Description Units Cat No Cylinder graduated polypropylene 500 ml each 1081 49 Distillation Heater and Support Apparatus 15V each 22744 00 Distillation Heater and Support Apparatus 230 Vue each 22744 02 Distillation Set General Purpose AA each 22653 00 Filter Paper folded 12 5 cm 100 box 1894 57 Flask Erlenmeyer polypropylene 500 ML each 1082 49 Flask volumetric Class A 100 mt each 14574 42 Flask volumetric Class A 1000 mt each 14574 53 Funnel polypropylene 65 mm each 1083 67 sensl ON 1 Basic Portable pH Meter with electrode each 51700 10 Pipet Filler safety bulb AN each 14651 00 Pipet Tensette 0 1 to 1 0 Macia cirio caracas each 19700 01 Pipet Tips for 19700 01 Tensette Pipet AA 50 pkg 21856 96 Pipet volumetric Class A A Dm NENNEN each 14515 04 Pipet volumetric Class A 8 0 ML eac2. after correct battery installation When replacing discharged batteries always replace the complete set of four alkaline batteries Rechargeable batteries are not recommended and cannot be recharged in the instrument Note The Low Battery icon will appear on the display when the batteries have 10 battery life remaining The battery icon will flash when the batteries are too low to complete measurements See Instrument Keys and Display on page 1 13 2 6 Instrument Operation continued Figure 1 Battery Installation 2 8 Error Codes When the instrument cannot perform the function initiated by the operator an error message will appear in the display Refer to the appropriate message information below to determine what the problem is and how it can be corrected Resolve error messages in the order that they appear on the display Service Centers are listed in page 2 37 Error Messages 1 E O No Zero User mode Error occurs when trying to read a standard in the user calibration mode before setting the meter to zero Zero the instrument on an appropriate blank 2 E 1 Ambient Light Error There is too much light present to take a valid measurement e Verify instrument cap is correctly seated e If the problem persists contact a Service Center page 2 37 Error Codes continued 3 E 2 LED Error The LED light source is out of regulation Replace batteries Verify LED lights up inside the cel
3. It is always good practice to wear safety glasses when handling chemicals Follow instructions carefully Rinse thoroughly if contact occurs If you have questions about reagents or procedures please contact the manufacturer or distributor Use of Hazard Information If multiple hazards exist this manual will use the signal word Danger Caution Note corresponding to the greatest hazard 1 7 Safety Precautions continued DANGER Indicates a potentially or imminently hazardous situation which if not avoided could result in death or serious injury CAUTION Indicates a potentially hazardous situation that may result in minor or moderate injury NOTE Information that requires special emphasis Precautionary Labels Please pay particular attention to labels and tags attached to the instrument Personal injury or damage to the instrument could occur if not observed A This symbol if noted on the instrument references the instruction manual for operational and or safety information 1 8 Specifications Lamp Light emitting diode LED Detector Silicon photodiode Photometric Precision 0 0015 Abs Filter bandwidth 15 nm Wavelength 655 nm Absorbance range 0 2 5 Abs Dimensions 3 2 x 6 1 x 15 2 cm 1 25 x 2 4 x 6 inches Weight 0 2 kg 0 43 Ibs Sample cells 25 mm 10 mL 1 cm 10 mL Operating conditions 0 to 50 C 32 to 122 F 0 to 90 relative humidity noncondensing Power supply Four AA
4. M ono continued 7 Calculate the amount of Chlorine Solution to be added to the ammonia standard using the following equation mL chlorine solution required free chlorine concentration 8 Open an ampule and use a glass Mohr pipet to add the calculated amount of Chlorine Solution slowly to the ammonia standard while mixing at medium speed on a stir plate 9 Allow the monochloramine solution to mix for 1 minute after all the Chlorine Solution is added 10 Quantitatively transfer the monochloramine solution to a clean 100 mL Class A volumetric flask Dilute to the mark with Organic Free Water cap and mix thoroughly This is a nominal 2 7 mg L as Cl monochloramine standard Use this standard within 1 hour of preparation Method Performance Precision Typical Precision 95 Confidence Interval 2 25 0 04 mg L as Cl 1 40 Chloramine M ono continued Estimated Detection Limit EDL 0 04 mg L as Cl Standard Calibration Adjust Method To perform a standard calibration adjustment using the Standard Solution prepared above or using an alternative concentration see Standard Calibration Adjust on page 2 13 Interferences The following have been tested for interference and found not to interfere up to the indicated levels in mg L Table 1 Substances that Do Not Interfere Substance Maximum Level Tested Alanine 1 mg L as N Aluminum 10 mg L Bromide 100 mg L 1 41 Chlorami
5. 0 60 mg L ammonia nitrogen standard or using an alternative concentration see Standard Calibration Adjust on page 2 13 1 26 Ammonia Nitrogen continued Interferences The following ions may interfere when present in concentrations exceeding those listed below Calcium 1000 mg L as CaCO3 Magnesium 6000 mg L as CaCO Nitrite 12 mg L as NO N Nitrate 100 mg L as NO3 N Orthophosphate 100 mg L as PO 3 P Sulfate 300 mg L as S042 e Sulfide will intensify the color Eliminate sulfide interference as follows a Measure about 350 mL of sample in a 500 mL Erlenmeyer flask b Add the contents of one Sulfide Inhibitor Reagent Powder Pillow Swirl to mix c Filter the sample through a folded filter paper d Use the filtered solution as the sample in step 1 of the procedure 1 27 Ammonia Nitrogen continued e Iron interferes with the test Eliminate iron interference as follows a Determine the amount of iron in the sample using a total iron analysis b Add the same amount of iron to the deionized water used in the procedure for the blank The interference from iron is successfully blanked Extremely acidic or alkaline samples should be adjusted to approximately pH 7 Use 1 0 N Sodium Hydroxide Standard Solution for acidic samples or 1 0 N Sulfuric Acid Standard Solution for basic samples Less common interferences such as hydrazine and glycine will cause intensified colors in the prepared sampl
6. 9 Flash Error The meter is unable to save data If the problem persists contact a Service Center page 2 37 2 11 Error Codes continued 8 Underrange flashing number below stated test range e Verify instrument cap is correctly seated e Check zero by measuring a blank If error recurs re zero the instrument e If the problem persists contact a Service Center page 2 37 Note See Maximum Minimum Displayed Value on page 2 26 for more information 9 Overrange flashing number above stated test range Note Flashing value will be 10 over the upper test limit Check for light blockage e Dilute and retest sample Note See Maximum Minimum Displayed Value on page 2 26 for more information 2 12 Standard Calibration Adjust The Pocket Colorimeter II instrument is factory calibrated and ready for use without user calibration Use of the factory calibration is recommended unless the user is required to generate a calibration The Standard Calibration Adjust can be used to meet regulatory requirements This feature allows the factory default calibration curve to be adjusted with a known standard Use the standard described in the procedure 1 ES Place a blank in the meter in measurement mode Press ZERO SCROLL Place the reacted standard in the meter Press READ ENTER Press MENU then press ZERO SCROLL until the display shows SCA Press READ ENTER to display the standard calibration adjust v
7. MIN SEC TT VT 8 Wait 15 minutes 9 After 15 minutes invert both cells a few times to mix 1 21 Ammonia Nitrogen continued 10 Press the POWER key 11 Place the yellow 12 Cover the blank with to turn the meter on colored blank in the cell the instrument cap The arrow should indicate holder mg L NH3 N Note See page 2 4 for information on selecting the correct range channel 1 22 Ammonia Nitrogen continued 13 Press ZERO SCROLL 14 Place the prepared 15 Press READ ENTER The display will show sample in the cell holder The display will show then 0 00 Cover the sample cell with followed by results in Remove the blank from the the instrument cap mg L ammonia as nitrogen cell holder NH3 N 1 23 Ammonia Nitrogen continued See Table 2 16 Multiply the result from step 15 by the appropriate factor from Table 2 if a dilution was used in steps 1 2 Note Results may be expressed as mg L ammonia NH3 or mg L ammonium NH4 by multiplying the NH3 N result by 1 22 or 1 29 respectively Table 2 Multiplication Factors mL Used in Step 1 Multiplier 10 0 25 5 0 5 0 25 10 0 1 0 25 0 1 24 Ammonia Nitrogen continued Sampling and Storage Collect samples in clean glass or plastic bottles If chlorine is present add one drop of 0 1 N Sodium Thiosulfate for each 0 3 mg L Cl2 in a 1 liter sample Preserve the sa
8. equal the factory calibrated values and cannot be changed 2 27 2 28 Certification Hach Company certifies this instrument was tested thoroughly inspected and found to meet its published specifications when it was shipped from the factory The Pocket Colorimeter II instrument has been tested and is certified as indicated to the following instrumentation standards EMC Immunity Per 89 336 EEC EMC EN 61326 1998 Electrical Equipment for measurement control and laboratory use EMC requirements Supporting test records by Hach Company certified compliance by Hach Company Standard s include IEC 1000 4 2 1995 EN 61000 4 2 1995 Electro Static Discharge Immunity Criteria B IEC 1000 4 3 1995 EN 61000 4 3 1996 Radiated RF Electro Magnetic Field Immunity Criteria A Additional Immunity Standard s include ENV 50204 1996 Radiated Electromagnetic Field from Digital Telephones 2 29 Certification continued Criteria A Radio Frequency Emissions Per 89 336 EEC EMC EN 61326 1998 Electrical Equipment for measurement control and laboratory use EMC requirements Class B emission limits Supporting test records from Hach EMC Test Facility certified compliance by Hach Company Additional Radio Frequency Emissions Standard s include EN 55022 CISPR 22 Class B emissions limits Canadian Interference causing Equipment Regulation IECS 003 Class A Supporting test records from H
9. existing calibration Used to enter a predetermined calibration curve e dFL Used to return the instrument back to the default factory calibration User entered calibrations are stored upon exit from the calibration or edit modes Note To return to factory settings following the instructions in Retrieving the Factory Calibration on page 2 25 If the instrument is shut off or loses power during data entry all edits will be lost Automatic shut off in user entered calibration entry mode is 60 minutes CAL and Edit Submenus In CAL mode standard values are entered and absorbance values are measured In Edit mode standard and absorbance values are entered e To select CAL from the User menu press READ ENTER e To select Edit from the User menu press ZERO SCROLL and READ ENTER 2 16 User Entered Calibration continued 0 Once in the CAL or Edit option press the READ ENTER key to navigate through each option Note Press ZERO SCROLL to quickly scroll through each option Calibration Procedure Using Prepared Standards Note Deionized water or a reagent blank can be used to zero during the calibration procedure Calibrations generated with deionized water as the zero will give less accurate results if the reagent blank is significantly more turbid or colored than deionized water Use the deionized water or the reagent blank as the zero concentration point S0 in the following calibration procedure 1 Turn on the instr
10. green color will develop if ammonia nitrogen is present e Wipe the cells before inserting into the sample cell compartment Note The Pocket Colorimeter II is designed to measure solutions contained in sample cells DO NOT dp the meter in the sample or pour the sample directly into the cell holder Adapted from Clin Chim Acta 14 403 1996 1 17 Ammonia Nitrogen continued Table 1 Recommended Sample Volumes Expected Concentration mg L NH3 N Sample Volume mL 0 01 0 80 No dilution necessary Use 10 0 mL directly in step 3 0 01 2 00 10 0 0 01 4 00 5 0 0 01 8 00 2 5 0 01 20 00 1 0 1 18 L Fill a 25 mL mixing graduated cylinder with the appropriate sample volume from Table 1 Ammonia Nitrogen continued 2 Dilute to 25 mL with ammonia free deionized water Stopper and invert three times to mix 1 19 i 3 Fill a 10 mL sample cell with 10 mL of sample from the graduated cylinder This will become the prepared sample Ammonia Nitrogen continued DP OG HRS MIN SEC _ II ES Bee 4 Fill a second 10 mL cell 5 Add the contents of one 6 Wait 3 minutes with 10 mL of ammonia Ammonia Salicylate Powder free deionized water This Pillow to each cell Cap and will become the blank shake to mix 1 20 7 Add the contents of one Ammonia Cyanurate Powder Pillow to each cell Cap and shake to dissolve Ammonia Nitrogen continued HRS
11. 59572 88 D POCKET COLORIMETER II ANALYSIS SYSTEMS INSTRUCTION MANUAL Ammonia Monochloramine Hach Company 2006 All rights reserved Printed in the U S A te dk 12 06 3ed Important Note This manual is intended for use with the following Pocket Colorimeter II instruments Ammonia Cat No 59530 40 M onochloramine Cat No 59530 14 The Pocket Colorimeter II instruments listed above are not interchangeable Table of Contents Safety Era 1 7 Laboratory Safety A 1 7 Use of Hazard Information 1 7 Precautionary Label 1 8 SBECIEICALIONS en IA 1 9 Instrument Keys and Display 1 13 Instrument Cap Cord WE 1 14 Ammonia Nitrogen ENEE 1 17 Measuring Hi 24 sainiadadtiauaitaiaiidiraiand iA E ai 1 17 Sampling and Storage a ea 1 25 Accuracy Check EE EE 1 25 Method Performance ENNEN 1 26 Standard Calibration Adjust Method AAA 1 26 ln Gg 1 27 Summary of Method NENNEN 1 29 Reagents and Apparatus cect cesses cesses 1 29 Table of Contents continued Chloramine M ong 1 33 MGaSUriNG HINtS iaa A eessen 1 33 Sampling and Storage EE 1 38 Accubacy Ene la a a a 1 38 Method Performance ENEE 1 40 Standard Calibration Adjust Method 1 41 Interferences un essen been 1 41 Summary of Method 1 46 Replacement Parts abris rta deu 1 46 Instrument Operation 23 Key Functons ENNEN 23 Menu Selections mini EE 24 Switching Ranges NNN 24 Setting Rut 24 Recalling Stored NMeas
12. A alkaline batteries approximate life is 2000 tests Backlight usage will decrease battery life 1 9 1 10 OPERATION DANGER Handling chemical samples standards and reagents can be dangerous Review the necessary Material Safety Data Sheets and become familiar with all safety procedures before handling any chemicals DANGER La manipulation des Echantillons chimiques etalons et r actifs peut tre dangereuse Lire les Fiches de Donn es de S curit des Produits FDSP et se familiariser avec toutes les proc dures de s curit avant de manipuler tous les produits chimiques PELIGRO La manipulaci n de muestras quimicas est ndares y reactivos puede ser peligrosa Revise las fichas de seguridad de materiales y familiaricese con los procedimientos de seguridad antes de manipular productos quimicos GEFAHR Das Arbeiten mit chemischen Proben Standards und Reagenzien ist mit Gefahren verbunden Es wird dem Benutzer dieser Produkte empfohlen sich vor der Arbeit mit sicheren Verfahrensweisen und dem richtigen Gebrauch der Chemikalien vertraut zu machen und alle entsprechenden Materialsicherheitsdatenblatter aufmerksam zu lesen PERIGO A manipulac o de amostras padr es e reagentes qu micos pode ser perigosa Reveja a folha dos dados de seguranca do material e familiarize se com todos os procedimentos de seguranga antes de manipular quaisquer produtos qu micos PERICOLO La manipolazione di campioni standard e reattivi ch
13. ach EMC Test Facility certified compliance by Hach Company This Class A digital apparatus meets all requirements of the Canadian Interference causing Equipment Regulations Cet appareil num rique de la classe A respecte toutes les exigences du Reglement sur le mat riel brouilleur du Canada FCC Part 15 Class A Limits Supporting test records from Hach EMC Test Facility certified compliance by Hach Company 2 30 Certification continued This device complies with Part 15 of the FCC Rules Operation is subject to the following two conditions 1 This device may not cause harmful interference and 2 This device must accept any interference received including interference that may cause undesired operation Changes or modifications to this unit not expressly approved by the party responsible for compliance could void the user s authority to operate the equipment This equipment has been tested and found to comply with the limits for a Class A digital device pursuant to Part 15 of the FCC Rules These limits are designed to provide reasonable protection against harmful interference when the equipment is operated in a commercial environment This equipment generates uses and can radiate radio frequency energy and if not installed and used in accordance with the instruction manual may cause harmful interference to radio communications Operation of this equipment in a residential area is likely to cause harmful interferen
14. alue Press READ ENTER to adjust the curve to the displayed value The meter will return to the measurement mode and the Calibration Adjusted icon will appear in the display window If an alternate concentration is used or if a standard concentration is not given 6 Repeat steps 1 4 2 13 Standard Calibration Adjust continued 7 Press ZERO SCROLL to access the Edit function then press READ ENTER to begin editing The digit to be edited will flash Use the ZERO SCROLL key to change the entry then press READ ENTER to accept and advance to the next digit When the last digit is entered press READ ENTER and the meter will adjust the curve to the value entered The meter will return to measurement mode and the Calibration Adjusted icon will appear in the display window To turn off Standard Calibration Adjust SCA 1 2 3 4 Press MENU Press ZERO SCROLL until SCA appears in the display Press READ ENTER then press ZERO SCROLL until Off appears in the display Press READ ENTER to turn off SCA Note Perform another standard calibration adjust to turn SCA on again Note For meters with factory calibrated ranges or methods Standard Calibration Adjust SCA will be disabled when a user entered method is programmed into the meter To turn SCA back on restore the meter to factory default calibration See Retrieving the Factory Calibration on page 2 25 2 14 User Entered Calibration Ov
15. anty Hach Company warrants this product to the original purchaser against any defects that are due to faulty material or workmanship for a period of two years from date of shipment In the event that a defect is discovered during the warranty period Hach Company agrees that at its option it will repair or replace the defective product or refund the purchase price excluding original shipping and handling charges Any product repaired or replaced under this warranty will be warranted only for the remainder of the original product warranty period This warranty does not apply to consumable products such as chemical reagents or consumable components of a product such as but not limited to lamps and tubing Contact Hach Company or your distributor to initiate warranty support Products may not be returned without authorization from Hach Company 2 38 Warranty continued Limitations This warranty does not cover damage caused by acts of God natural disaster labor unrest acts of war declared or undeclared terrorism civil strife or acts of any governmental jurisdiction damage caused by misuse neglect accident or improper application or installation damage caused by any repair or attempted repair not authorized by Hach Company any product not used in accordance with the instructions furnished by Hach Company freight charges to return merchandise to Hach Company freight charges on expedited or express shipment of
16. appears Note If the meter does not display USER followed by CAL after pressing the MENU key the factory calibration cannot be modified on this channel Press the ZERO SCROLL key to find dFL Press the READ ENTER key to select dFL and restore the instrument to the factory default calibration 2 25 User Entered Calibration continued Note For meters with factory calibrated ranges or methods Standard Calibration Adjust SCA will be disabled when a user entered method is programmed into the meter To turn SCA back on restore the meter to factory default calibration Maximum M inimum Displayed Value In meters with absorbance Abs ranges the maximum displayed value and minimum displayed value is related to the value of the standards entered in a user calibration Measurements that exceed the minimum or maximum standards entered in the user calibration will return a flashing number indicating underrange or overrange See Error Codes page 2 12 for more information Example 1 For a calibration with the following standards S0 0 000 51 1 000 Maximum Displayed Value 1 000 Minimum Displayed Value 0 000 2 26 User Entered Calibration continued Example 2 For a calibration with the following standards 50 1 00 1 2 00 52 4 00 Maximum Displayed Value 4 00 Minimum Displayed Value 1 00 For Hach calibrated programs the maximum and minimum displayed values always
17. are za 500 mL 24065 49 Chlorine Solution Voluette Ampule cesses 16 pkg 14268 10 Optional Apparatus Description Unit Cat No Beaker 100 Mistica teten each 500 42H Flask Volumetric Class A 100 ml each 14574 42 1 47 D Section 2 Instrument M anual Instrument Operation Key Functions Key Description Function POWER On Off Backlight To turn on the backlight turn on the instrument then press and hold the power key until the backlight turns on Press and hold again to turn off the backlight This key functions the same in all instrument modes and ranges ZERO SCROLL In measurement mode sets the instrument to zero In menu mode scrolls through menu options Also scrolls numbers when entering or editing a value READ ENTER In measurement mode initiates sample measurement In menu mode selects a menu option When entering numbers moves one space to the right and executes the function when the entry is complete 2 3 Instrument Operation continued Key Description Function MENU Enter Exit the menu mode Press and hold for approximately 5 seconds to enter user entered method mode Menu Selections Press the MENU key to access the menu selections Switching Ranges 1 Press the MENU key The display will show SEL A flashing arrow indicates the current range 2 Press the READ ENTER key to toggle between range
18. aries inversely with the temperature of the sample Use this table to determine the minimum color development time for a sample Table 3 Color Development Time Based on Sample Temperature Sample Temperature dere F 5 40 10 42 9 9 48 8 10 50 8 12 54 1 14 58 1 16 61 6 18 68 4 20 73 3 23 75 2 1 45 Chloramine M ono continued Table 3 Color Development Time Based on Sample Temperature Continued Sample Temperature C F 25 77 2 Time minutes Summary of Method In the presence of a cyanoferrate catalyst monochloramine in the sample reacts with a substituted phenol to form an intermediate monoimine compound The intermediate couples with excess substituted phenol to form a green colored indophenol which is proportional to the amount of monochloramine present in the sample Replacement Parts Required Reagents Description Unit Cat No Monochlor F Reagent Pillows nsss 50 pkg 28022 46 Chloramine M ono continued Required Apparatus Using AccuVac A mpuls Description Unit Cat No Sample Cell 1 CM 1O mt 2 pkg 48643 02 Clippers SAC uva a Ada each 23694 00 Optional Reagents Rochelle Salt Solution uesseeeenenneeeenennnenennnnnnennenennnennen 29 mL DB 1725 33 Organic Free Water e Seed dE ec 500 mL 26415 49 Buffer Powder PillOWS ENEE 25 pKQ 898 68 Nitrogen Ammonia Standard Solution 100 m9 Las N Ha3 N
19. ce in which case the user will be required to correct the interference at his own expense The following techniques of reducing the interference problems are applied easily 2 31 1 Certification continued Remove power from the Pocket Colorimeter instrument by removing one of ts batteries to verify that it is or is not the source of the interference Move the Pocket Colorimeter instrument away from the device receiving the interference Reposition the receiving antenna for the device receiving the interference Try combinations of the above 2 32 D GENERAL INFORM ATION At Hach Company customer service is an important part of every product we make With that in mind we have compiled the following information for your convenience 2 33 2 34 How to Order By Telephone By Mail 6 30 a m to 5 00 p m MST Hach Company Monday through Friday P O Box 389 800 227 HACH 800 227 4224 Loveland Colorado 80539 0389 U S A By FAX For order information by E mail 970 669 2932 Hach Loveland orders www hach com Information Required e Hach account number if available Purchase order number Billing address e Catalog number e Shipping address e Brief description or model number e Your name and phone number e Quantity 2 35 How to Order continued Technical and Customer Service USA only Hach Technical and Customer Service Department personnel are eager to answer questions about our prod
20. determined Calibration Curve Note Entering a predetermined calibration curve requires at least two data pairs Each data pair requires a concentration value and the absorbance value for the given concentration Up to 10 data pairs may be entered This procedure uses the Edit mode 1 Turn on the instrument and select the range to be calibrated An arrow at the top of the display will point to the selected range To change ranges press the MENU key then use the READ ENTER key to toggle between ranges 1 and 2 Press MENU again to return to measurement mode 2 Press the MENU key and hold it down until the display shows USER followed by CAL Press ZERO SCROLL to scroll to EDIT Press READ ENTER 3 In uncalibrated meters or in Abs range RES will appear Press ZERO SCROLL To change the resolution decimal placement press READ ENTER Press ZERO SCROLL to select the new resolution then press READ ENTER to accept SO will appear on the display 2 20 User Entered Calibration continued Enter the concentration value and absorbance value of the first data pair S0 AO To enter the SO value press READ ENTER Use the ZERO SCROLL key to select the numerical value then press the READ ENTER key to accept the entry and advance to the next decimal place Repeat this sequence until the SO concentration value is entered After editing the SO value press READ ENTER to accept A0 will appear on the display T
21. e Turbidity and sample color will give erroneous high values Samples with severe interferences require distillation Albuminoid nitrogen samples also require distillation Hach recommends the distillation procedure using the Hach General Purpose Distillation Set See Optional Apparatus on page 1 31 1 28 Ammonia Nitrogen continued Summary of Method Ammonia compounds combine with chlorine to form monochloramine Monochloramine reacts with salicylate to form 5 aminosalicylate The 5 aminosalicylate is oxidized in the presence of a sodium nitroprusside catalyst to form a blue colored compound The blue color is masked by the yellow color from the excess reagent present to give a final green colored solution Reagents and Apparatus Required Reagents and Apparatus Using Powder Pillows Description Units Cat No Ammonia Nitrogen Reagent Set 10 mL sample 100 tests and blanks AN 26680 00 Includes 2 Ammonia Cyanurate Reagent Powder Pillows 2 Ammonia Salicylate Powder Pillows Cylinder graduated mixing 25 ML eeh each 20886 40 1 29 Ammonia Nitrogen continued Optional Reagents Description Units Nitrogen Ammonia Standard Solution NIST 10 MglL a5 NHN anne 500 mL Nitrogen Ammonia Voluette A mpules NIST 50 mg L as NH3 N Dm 16 pkg Sodium Hydroxide Standard Solution 1 0 N une 100 mL MDB Sodium Hydroxide Standard Solution BA DN 59 mL Sodium Thiosulfate Standard Solution 0 1 N
22. e calibration points or press MENU twice to return to the measurement mode Note When a factory calibration curve has been edited the calibration adjust icon will appear in the display Exiting the Calibration Routine Exit the calibration routine by pressing the MENU key to return to measurement mode The instrument uses the last completed user entered calibration or the factory calibration if no user entered calibration has been completed Deleting Calibration Points 1 Select the range containing user entered calibration points See Switching Ranges on page 2 4 2 Press and hold the MENU key until USER then CAL appears Press READ ENTER Note Calibration points can also be deleted in Edit mode 3 Press ZERO SCROLL to select the point to delete e g SO or S1 or S2 Press READ ENTER 2 24 6 User Entered Calibration continued The left digit will flash Press ZERO SCROLL until dEL appears dEL will appear after the numeral 9 Press READ ENTER to delete Repeat for all points to be deleted Note The minimum number of valid points is two For example if five points have been entered three can be deleted using this feature Press MENU to return to the measurement mode Retrieving the Factory Calibration 1 2 Select the range to restore factory default calibration See Switching Ranges on page 2 4 Press and hold the MENU key until USER then CAL
23. er 10 mL 1 cm instrument cap The display will show cell to the 10 mL line with 22 then 0 00 sample Add the contents of one Monochlor F Powder Remove the blank from the Pillow to the sample cell cell holder 1 35 Chloramine M ono continued 7 Cap the cell and shake 8 Wait five minutes Place 9 Cover the cell with the for 20 seconds the prepared sample in the instrument cap cell holder Note Samples colder than 18 C will require additional reaction time See Table 3 on page 1 45 for correct times Note Wipe liquid off sample cells 1 36 Chloramine M ono continued 10 Press READ ENTER ihe display will show followed by results in mg l chlorine 1 37 Chloramine Mono continued Sampling and Storage Analyze samples for monochloramine immediately after collection If sampling with the sample cell rinse the cell several times with the sample then carefully fill to the 10 mL mark If sampling from a tap let the water flow for at least 5 minutes Let the container overflow with the sample several times then cap the container so there is no headspace air above the sample Accuracy Check Standard Solutions M ethod Standard solutions for monochloramine are difficult and time consuming to prepare Errors can occur if attention to detail is not addressed during preparation of the standards Hach Company prepares the calibration curve under rigorous analytical laborator
24. erview The Pocket Colorimeter II will accept a user prepared calibration curve The curve can extend from 0 to 2 5 absorbance A user prepared calibration curve may be entered into any channel that does not contain a factory programmed curve These channels are labeled abs on instruments having a single factory calibration or are labeled 1 and 2 on the uncalibrated single wavelength instruments Any chemistry that can be run at the instrument wavelength may be user entered in these channels Using prepared standard solutions that cover the range of interest the meter generates a calibration curve by calculating the straight line segments between each standard entered A calibration curve may be entered using the keypad Factory entered calibration curves may also be recalculated or adjusted using the same procedure To enter the user entered calibration mode press the MENU key and hold it down until the display shows USER about 5 seconds followed by CAL Press ZERO SCROLL to scroll through the options Note If the meter does not display USER followed by CAL after pressing the MENU key the factory calibration cannot be modified on this channel 2 15 User Entered Calibration continued e cCAL Used to enter and edit standard values and measure absorbance values or review the existing calibration e Edit Used to enter and edit standard values and absorbance values with the keypad or review the
25. h 14515 08 Thermometer 10 to 110 C oie testes ieee each 1877 01 1 31 Ammonia Nitrogen continued Replacement Parts Description Units Cat No Batteries AAA alkaline A 4 pkg 46743 00 Instrument Cap light held each 59548 00 Instruction Manual Ammonia MonochloraMiNe cesses each 59572 88 Sample Cell 10 ML With Cap 6 pkg 24276 06 1 32 Chloramine M ono 0 04 to 4 50 mg L as Cl Method 10171 For chloraminated drinking water and chlorinated wastewater Indophenol Method Measuring Hints e Analyze samples immediately Do not preserve for later analysis Note The Pocket Colorimeter II is designed to measure solutions contained in sample cells DO NOT dp the meter in the sample or pour the sample directly into the cell holder U S Patent No 6 315 950 1 33 Chloramine M ono continued L Press the POWER key to turn the meter on The arrow should indicate mg L Clo Note See page 2 4 for information on selecting the correct range channel 2 Fill the 10 mL 1 cm cell to the 10 mL line with sample Note Samples must be analyzed immediately and cannot be preserved for later analysis 1 34 3 Remove the instrument cap and placethe cell in the cell holder with the triangle facing the keypad Note Wipe excess liquid and fingerprints from the sample cells Chloramine M ono continued 4 Cover the cell with the 5 Press ZERO SCROLL 6 Fill anoth
26. imici pu essere pericolosa La preghiamo di prendere conoscenza delle Schede Techniche necessarie legate alla Sicurezza dei Materiali e di abituarsi con tutte le procedure di sicurezza prima di manipolare ogni prodotto chimico 1 1 1 12 Instrument Keys and Display Item Description he POWER BACKLIGHT Key BO ZERO SCROLL Key MENU Key Numeric Display Range Indicator Range Indicator Menu Indicator Calibration Adjusted Indicator oo o o a Pi Ww FA Battery Low Indicator pi o READ ENTER Key 1 13 Instrument Cap Cord The instrument cap for the Pocket Colorimeter II doubles as a light shield Accurate measurements cannot be obtained unless the sample or blank is covered with the cap Use the instrument cap cord to secure the cap to the body of the colorimeter and prevent loss of the cap See Figure 1 on page 1 15 L Loop the instrument cap cord through the ring on the cap 2 Remove the battery compartment cover Press the knotted end of the cord into the hole indicated by the arrow 3 Slide the cord into the slot on the battery compartment cover Snap the cover into place 1 14 Instrument Cap Cord continued Figure 1 Attaching the Instrument Cap Cord A 1 15 1 16 Ammonia Nitrogen 0 01 to 0 80 mg L NH3 N Method 8155 For water wastewater and seawater Salicylate Method Measuring Hints e A
27. in Abs range RES will appear Press ZERO SCROLL to review the current resolution Press ZERO SCROLL again to accept the displayed resolution To change the resolution decimal placement press READ ENTER Press ZERO SCROLL to select the new resolution then press READ ENTER to accept S0 will appear on the display 2 22 10 User Entered Calibration continued Press READ ENTER The current concentration value for SO will appear on the display To edit the SO value press READ ENTER Use the ZERO SCROLL key to select the numerical value then press the READ ENTER key to accept the entry and advance to the next decimal place Repeat this sequence until the SO concentration value is entered After editing the SO value press READ ENTER to accept A0 will appear on the display To edit the absorbance value for SO press the READ ENTER key to go to entry mode Use the ZERO SCROLL key to select the numerical value then press the READ ENTER key to accept the entry and advance to the next decimal place Repeat this sequence until the absorbance value for SO is entered After editing AO press READ ENTER to accept S1 will appear on the display Repeat steps 4 through 8 for each standard value and absorbance value pair in the calibration curve When all calibration data has been reviewed or edited ADD will appear in the display 2 23 User Entered Calibration continued 11 Press READ ENTER to add mor
28. l holder when the READ ENTER or ZERO SCROLL key is pressed e If the problem persists contact a Service Center page 2 37 Note When an E 1 or E 2 error occurs on a measurement the display will show __ The decimal place is determined by the chemistry If the E 1 or E 2 error occurs while zeroing the meter the meter will require the user to re zero E 3 Standard Adjust Error The value obtained on the prepared standard exceeds the adjustment limits allowed for the standard concentration or the concentration of the standard is outside the concentration range allowed for standard calibration adjust Prepare the standard and rerun according to the procedure Preparea standard at or near the recommended concentrations given in the procedure Verify that the concentration of the standard has been entered correctly 2 10 5 Error Codes continued e If the problem persists contact a Service Center page 2 37 E 6 Abs Error User mode Indicates that the absorbance value is invalid or indicates an attempt to make a curve with less than two points Enter or measure the absorbance value again e If the problem persists contact a Service Center page 2 37 E 7 Standard Value Error User mode Standard concentration is equal to another standard concentration that is already entered Enter the correct standard concentration If the problem persists contact a Service Center page 2 37 E
29. mple by reducing the pH to 2 or less with sulfuric acid at least 2 mL Store at 4 C 39 F or less Preserved samples may be stored up to 28 days Warm samples to room temperature Neutralize with 5 0 N Sodium Hydroxide before analysis Correct the test result for volume additions by multiplying by final volume original volume plus additions and dividing by original volume Accuracy Check Standard Additions M ethod 1 Measure 25 mL of sample into each of three 25 mL mixing cylinders 2 Use a TenSette pipet to add 0 2 0 4 and 0 6 mL of Ammonia Nitrogen Standard 10 mg L as NH3 N to the three samples respectively Mix well 3 Analyze a 10 mL portion of each solution as described in the procedure The ammonia nitrogen concentration should increase 0 08 mg L for each 0 2 mL of standard added using the procedure without any sample dilution 1 25 Ammonia Nitrogen continued Standard Solution Method Prepare a 0 60 mg L NH3 N standard by pipetting 6 0 mL of a 10 mg L Ammonia Nitrogen Standard into a 100 mL volumetric flask Dilute to 100 mL with ammonia free water Use 10 mL of this 0 60 mg L ammonia nitrogen standard directly in step 3 of the procedure Perform the procedure as described Method Performance Precision Typical Precision 95 Confidence Interval 0 60 0 05 mg L NH3 N Estimated Detection Limit EDL 0 01 mg L NH3 N Standard Calibration Adjust Method To perform a standard calibration adjustment using the
30. ne Mono continued Table 1 Substances that Do Not Interfere Continued Substance Maximum Level Tested Bromine 15 mg L Calcium 1000 mg L CaCO3 Chloride 18 000 mg L Chlorine Dioxide 5 mg L CIO Chromium 3 5 mg L Copper 10 mg L Cyanide 10 mg L Free Chlorine 10 mg L as Cl Glycine 1 mg L as N Iron 2 10 mg L Iron 3 10 mg L Lead 10 mg L Chloramine M ono continued Table 1 Substances that Do Not Interfere Continued Substance Maximum Level Tested Nitrate 100 mg L as N Nitrite 50 mg L as N Phosphate 100 mg L PO Silica 100 mg L SiO Silver 10 mg L Sulfates 2600 mg L Sulfite 50 mg L S03 Tyrosine 1 mg L as N Urea 10 mg L as N Zinc 5 mg L Chloramine M ono continued The following may interfere when present at concentrations greater than listed Table 2 Substances that May Interfere Interfering Substance and Positive or Negative Effect Interference Level Recommended Treatment M agnesium Above 400 mg L CaCO Add 5 drops Rochelle Salt solution before testing Manganese Above 3 mg L present Ozone Above 1 mg L NA Usually doesn t co exist with monochloramine Sulfide Turns rust colored if NA Usually doesn t co exist with monochloramine Thiocyanate Above 0 5 mg L 1 44 Chloramine M ono continued Color development time v
31. o enter the absorbance value for SO press the READ ENTER key to go to entry mode Use the ZERO SCROLL key to select the numerical value then press the READ ENTER key to accept the entry and advance to the next decimal place Repeat this sequence until the absorbance value for SO is entered After entering AO press READ ENTER to accept S1 will appear on the display Repeat steps 5 through 8 for each standard value and absorbance value pair in the calibration curve Note After Al is entered Add will appear in the display If additional data pairs are to be entered press READ ENTER and continue with step 9 2 21 User Entered Calibration continued 10 When all the calibration data has been entered press MENU twice to return to the measurement mode Editing a User entered or Factory Calibration Curve 1 Press the MENU key and hold it down until the display shows USER followed by CAL Press ZERO SCROLL until EDIT appears Note If the meter does not display USER followed by CAL after pressing the MENU key the factory calibration cannot be modified on this channel 2 Press the READ ENTER key to enter Edit mode In factory calibrated meters S0 will appear in the display Note When editing a factory calibrated meter or range RES resolution cannot be changed Note When RES or SO appears in the display press ZERO SCROLL to quickly scroll to the data to be edited 3 In uncalibrated meters or
32. ove from digit to digit Use the ZERO SCROLL key to change the number After completing entry of the blank value press the READ ENTER key The display will show AO 2 18 10 11 12 13 14 15 User Entered Calibration continued Insert the reagent blank or deionized water into the cell holder Cover the blank with the instrument cap Press the READ ENTER key The meter will measure and display the absorbance value for SO Remove the sample blank Press the ZERO SCROLL key S1 will appear Press the READ ENTER key then enter the first standard value Note Press the READ ENTER key to move from digit to digit Use the ZERO SCROLL key to change the number After completing entry of the first standard value press the READ ENTER key The display will show A1 Insert the first reacted standard solution into the cell holder Cover the prepared standard with the instrument cap Press the READ ENTER key The meter will measure and display the absorbance value for S1 The calibration is complete with two points If additional standards are required press ZERO SCROLL until Add appears on the display Repeat steps 11 14 to enter additional standards 2 19 User Entered Calibration continued 16 Press the MENU key twice to exit and accept the changes The instrument will use this calibration to determine the displayed concentration of future sample measurements Entering a Pre
33. s 3 Press MENU again to accept and exit back to the measurement screen Setting the Time L Press the MENU key then press the ZERO SCROLL key until the display shows a time in the 00 00 format 24 Instrument Operation continued 2 Press READ ENTER The digit to be edited will flash 3 Use the ZERO SCROLL key to change the entry then press READ ENTER to accept and advance to the next digit The time is entered in 24 hour format Recalling Stored Measurements L Press the MENU key then press the ZERO SCROLL key until the display shows RCL The instrument automatically stores the last 10 measurements 2 In RCL press READ ENTER to recall the stored measurements beginning with the most recent measurement taken The meter stores the measurement number as 01 most recent through 10 oldest the time the measurement was taken and the measurement value The ZERO SCROLL key allows for selection of a specific measurement by number The READ ENTER key scrolls through all stored data points Instrument Operation continued A Battery Installation Figure 1 on page 2 7 provides an exploded view of battery installation 1 Unhook the latch and remove the battery compartment cover The polarities are shown on the battery holder 2 Placethe four batteries provided with the instrument in the holder as indicated and replace the battery compartment cover The display will show the software version number e g P 1 6
34. ucts and their use and to take your orders Specialists in analytical methods they are happy to put their talents to work for you Call 1 800 227 4224 or E mail techhelp hach com International Customers Hach maintains a worldwide network of dealers and distributors To locate the representative nearest you send E mail to intl hach com or call 970 669 3050 In Canada Hach Instrument Service Centre Winnipeg Manitoba Canada Telephone 204 632 5598 800 665 7635 FAX 204 694 5134 2 36 Repair Service Authorization must be obtained from Hach Company before sending any items for repair Please contact the Hach Service Center serving your location In the United States Hach Company 100 Dayton Avenue Ames lowa 50010 800 227 4224 USA only FAX 515 232 3835 Latin America Caribbean Africa Far East Indian Subcontinent Hach Company World Headquarters P O Box 389 Loveland Colorado 80539 0389 U S A Telephone 970 669 3050 FAX 970 669 2932 E mail intl hach com Canada Hach Sales amp Service Canada Ltd 1313 Border Street Unit 34 Winnipeg Manitoba R3H 0X4 800 665 7635 Canada only Telephone 204 632 5598 FAX 204 694 5134 E mail canada hach com Europe the Middle East or Mediterranean Africa HACH Company c o Dr Bruno Lange GmbH amp CO KG Willstatterstr 11 40549 Dusseldorf Germany Telephone 49 0 211 52 88 0 FAX 49 0 211 52 88 134 2 37 Warr
35. ument and select the range to be calibrated An arrow at the top of the display will point to the selected range To change ranges press the MENU Key then use the READ ENTER key to toggle between ranges 1 and 2 Press MENU again to return to measurement mode 2 Follow the procedure for the chemical method to be calibrated Prepare a reagent blank if needed and a standard solution Allow the color to develop fully 2 17 User Entered Calibration continued 3 Insert the reagent blank or deionized water into the meter and cover with the cap Press the ZERO SCROLL key The meter will display followed by 0 000 This initializes zeroes the meter Press the MENU key and hold it down until the display shows USER followed by CAL Press READ ENTER to enter the calibration mode In factory calibrated meters SO will appear in the display Note When recalibrating a factory calibrated meter or range RES resolution cannot be changed In uncalibrated meters or meters with ranges labeled Abs RES will appear Press ZERO SCROLL to review the current resolution decimal placement Press ZERO SCROLL again to accept the current resolution To change the resolution press READ ENTER then ZERO SCROLL to change the resolution Press READ ENTER to accept the new resolution SO will appear on the display Press the READ ENTER key again then enter the blank value Note Press the READ ENTER key to m
36. urements AAA 25 Battery nstall ations accstrsiaad ii eeh 2 6 Err Codes a o 29 EMOL MOS ae re see 2 9 Standard Calibration Adjust 2 13 Table of Contents continued User Entered Calibration 0 00 0 nn 2 15 OVERVIEW cann isn nei a RAR Rd ahnnlanGiin eat E 2 15 Calibration Procedure Using Prepared Standards AA 2 17 Entering a Predetermined Calibration Cunme ocoociccccincincnonrnnnororcnscrcrorcnnoss 2 20 Editing a User entered or Factory Calibration Curve 2 22 Exiting the Calibration Routine 2 24 Deleting Calibration Points 2 24 Retrieving the Factory Calibration sssrinin 2 25 Maximum M inimum Displayed Value 2 26 GEertification a see 229 HOW to Rekter ee 2 35 Repalf Service u 2 37 Warranty stane in olaan an ar i 2 38 1 6 Safety Precautions Please read this entire manual before unpacking setting up or operating this instrument Pay particular attention to all danger and caution statements Failure to do so could result in serious injury to the operator or damage to the equipment To ensure the protection provided by this equipment is not impaired do not use or install this equipment in any manner other than that which is specified in this manual Laboratory Safety As part of good laboratory practice please familiarize yourself with the reagents used in these procedures Read all product labels and the material safety data sheets MSDS before using them
37. warranted parts or product travel fees associated with on site warranty repair 2 39 Warranty continued This warranty contains the sole express warranty made by Hach Company in connection with its products All implied warranties including without limitation the warranties of merchantability and fitness for a particular purpose are expressly disclaimed Some states within the United States do not allow the disclaimer of implied warranties and if this is true in your state the above limitation may not apply to you This warranty gives you specific rights and you may also have other rights that vary from state to state This warranty constitutes the final complete and exclusive statement of warranty terms and no person is authorized to make any other warranties or representations on behalf of Hach Company Limitation of Remedies The remedies of repair replacement or refund of purchase price as stated above are the exclusive remedies for the breach of this warranty On the basis of strict liability or under any other legal theory in no event shall Hach Company be liable for any incidental or consequential damages of any kind for breach of warranty or negligence 2 40
38. y conditions Hach recommends using the factory calibration A user calibration or a user prepared monochloramine standard may be required by a regulatory official or agency Two options are available on the Pocket Colorimeter II to meet this requirement A monochloramine standard may be prepared and used to validate the calibration curve using the Standard Calibration Adjust feature see page 2 13 for more 1 38 Chloramine M ono continued information In addition a user generated calibration curve can be made and programmed into the Pocket Colorimeter Il See Calibration Procedure Using Prepared Standards on page 2 17 for more information about preparing a full calibration curve Standard Solution Preparation 1 Prepare the following monochloramine standard fresh before use 2 Add the contents of one pillow Buffer Powder pH 8 3 to about 50 mL of Organic Free Water in a clean 100 mL Class A volumetric flask Swirl to dissolve the powder 3 Using a Class A volumetric pipet transfer 2 00 mL of Ammonia Nitrogen Standard Solution 100 mg L as NH3 N into the flask 4 Dilute to volume with Organic Free Water cap and mix thoroughly This is a 2 00 mg L buffered ammonia standard 5 Pipet 30 00 mL of the buffered ammonia standard into a clean 100 mL beaker with a stir bar 6 Obtain a recent lot of Chlorine Solution Ampules 50 70 mg L and note the actual Free Chlorine concentration for this lot 1 39 Chloramine
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