Perfusion Valve Control System User`s Manual
Contents
1. The unit uses only one fuse the second one is sent as spare Follow these instructions to change the unit s from 120 VAC or 220 VAC Power Entry Module Fuse holder side view Push out drawer to access N the Spare Fuse Active Fuse Step 1 Step 2 Depending on AC Voltage being used turn VAC selector switch to 110 VAC or 220 VAC Publication 5712 001 REV B Carefully pry open the fuse holder from the inside by using a small flat screwdriver Replace fuse according to voltage being used Item 4630130 Model VC 6 and VC 6M use For 120 VAC 0 50 Amp 5 x 20 mm Slow Blow For 220 VAC 0 25 Amp 5 x 20 mm Slow Blow www warneronline com E Perfusion Valve Control System Model VC 6T Warner Instruments Operating Directions contd Front Panel The front panel of the VC 6 Valve Controller contains TTL inputs for each valve an associated 3 position toggle switch for manually setting the state of each valve and an LED displaying the active status for each valve There is also a 3 position toggle switch for selecting the command input mode and a power switch with power on LED Command input toggle A COMMAND INPUT TOGGLE SWITCH is provided for each of the 6 chan nels of the VC 6 Controller to allow selection between internal and external commands Placing a COMMAND INPUT TOGGLE SWITCH into the on position2. Perfusion Valve Control System Model VC 6T WARNER A INSTRUMENTS A Harvard Apparatus Company Publication 5712 001 REV B WEEE RoHS Compliance Statement EU Directives WEEE and RoHS To Our Valued Customers We are committed to being a good corporate citizen As part of that commitment we strive to maintain an environmentally conscious manufacturing operation The European Union EU has enacted two Directives the first on product recycling Waste Electrical and Electronic Equipment WEEE and the second limiting the use of certain substances Restriction on the use of Hazardous Substances RoHS Over time these Directives will be implemented in the national laws of each EU Member State Once the final national regulations have been put into place recycling will be offered for our products which are within the scope of the WEEE Directive Products falling under the scope of the WEEE Directive available for sale after August 13 2005 will be identified with a wheelie bin symbol Two Categories of products covered by the WEEE Directive are currently exempt from the RoHS Directive Category 8 medical devices with the exception of implanted or infected products and Category 9 monitoring and control instruments Most of our products fall into either Category 8 or 9 and are currently exempt from the RoHS Directive We will continue to monitor the application of the RoHS Directive to its products and will comply with any ch
3. VC GT by first attaching the VALVE BRACKET to the SUPPORT STAND Place the VALVE BRACKET near the base of the SUPPORT STAND as shown below This is followed by attaching the SYRINGE HOLDER to the top of the SUPPORT STAND Place the SYRINGE HOLDER near the top of the SUPPORT STAND as shown below Remove the plungers from the six supplied 60 cc syringes and place the syringes into the SYRINGE HOLDER Attach STOPCOCKS to each syringe Cut six 6 pieces of Teflon tubing long enough to run from the stopcocks to the input ports on the Teflon valves Attach one 1 blunt end 18 gauge syringe needle to both ends of each length of Teflon tubing to provide Luer connection points Insert a short section of PE 160 onto the needle tip to facilitate a tight seal between the Teflon tubing and the syringe needle Publication 5712 001 REV B www warneronline com E Perfusion Valve Control System Model VC 6T Warner Instruments Operating Directions conta 6 Using the Luer ended Teflon tubes formed in step 5 make a connec tion between the stopcock on each syringe to the associated input port on each Teflon valve Proper tubing lengths Luer connector attachments and tubing placements are shown in the montage below 7 Cut six 6 pieces of Teflon tubing to run from the output ports of the Teflon valve to the supplied MPP manifold The required tubing length is left to the discretion of the user but should be sufficient to al
4. anges as they apply Do Not Dispose Product with Municipal Waste Special Collection Disposal Required E Perfusion Valve Control System Model VC 6T Warner Instruments Table of Contents IntroductlOni Lt 2 xcu eem c PS 2 Operating Directions OAD sid iet te denuo Dat Rate du Daun 3 Components inania a ai tA e e adde 3 Al iia 4 6 A AAK MANA 6 O 7 Rear PANGI cui kala 8 Instructions 01080457 SNAP AAMU deb Por bane 9 FON AGUSE PANAMAS e e e ea e 9 Maintenance CM ts eat 10 Stuck or leaky valves oro 10 Wario ii 11 Specifications cti 12 Publication 5712 001 REV B www warneronline com Perfusion Valve Control System Model VC 6T Warner Instruments Introduction The VC 6T Valve Control System lies at the heart of a multi valve perfusion system designed to automate and control the delivery of solutions to Warner Instruments imaging and recording chambers In addition its flexible design allows the VC 6T to be used in many applications not using Warner equipment The complete system includes a valve controller a valve bracket with Teflon valves connecting cable an MPP 6 manifold a support stand syringe holder six 60 cc syringes 25 feet of Teflon tubing 10 feet of PE 160 18 blunt ended 18 gauge syringe needles and 6 stopcocks The controller can independently regulate the function of up to six valves Individual valves can be controlled via manual switch an external analog signal or an externa ldi
5. drives the associated valve into its open state A it LED indicates the open state of the valve In a similar manner placing a COMMAND INPUT TOGGLE SWITCH into the off position drives the associated valve into its closed state An unlit LED indicates the closed state of the valve TTL Inputs TTL inputs front panel BNC s are provided for external control for each valve channel Use of these inputs can allow for the simultaneous opening of more than one valve from a digital source e g a computer Placing a COMMAND INPUT TOGGLE SWITCH into the ext position acti vates the associated TTL BNC input A logic level low 0 V applied to the BNC places the associated valve into the closed state Correspondingly a Publication 5712 001 REV B www warneronline com Perfusion Valve Control System Model VC 6T Warner Instruments Operating Directions contd logic level high 3 to 5 V places the associated valve into the open state A lit and an unlit LED indicates the open and closed states of the valve respectively Rear Panel The rear panel contains the power input module with fuse an EVENT MARK ER OUTPUT and a 15 pin D connector for the VALVE CONTROL BRACKET To Valves A 15 pin D type female connector is used to connect the cable from the VALVE BRACKET to the CONTROLLER Event Out The Event Marker output produces a 500 ms logic level output 5 V each time a valve is turned on At all other times the Event Mark
6. el pulse 500 ms nominal rear panel BNC connector 2 1 4 1 or 6 1 nominal dead space 110 to 130 or 200 to 250 VAC single phase 50 60 Hz 20 W 10 to 40 C 50 to 104 F 89 x 203 x 305 mm 3 5 x 8 0 x 12 in 3 7 kg 8 lb 4 6 kg 10 Ib www warneronline com
7. er output is low 0 V Power input module A polarized 3 conductor IEC320 CEE 22 connector is used for line mains power input to the instrument A removable cordset terminated with a NEMA 5 15P connector is standard A fuse holder contains a protective fuse in series with the high side brown or black wire of the mains The holder accepts 5 x 20 mm fuses of the type indicated Publication 5712 001 REV B www warneronline com s Perfusion Valve Control System Model VC 6T Warner Instruments Operating Directions conta Instructions for Use Flow Adjustment Flow rates can be adjusted by raising or lowering the reservoir holder as well as by adjusting the height of each reservoir within the holder The table below lists the approximate flow rates for a reservoir at the spec ified height with the supplied Tygon tubing Reservoir height Approximate flow rate 61 cm 24 in 14 ml min CITED one Publication 5712 001 REV B www warneronline com Perfusion Valve Control System Model VC 6T Warner Instruments Maintenance Cleaning Do not use alcohol aromatic hydrocarbons or chlorinated solvents for cleaning They may adversely react with the plastic materials used to man ufacture the system If salt solution spills on the valve assembly it should be cleaned as soon as pos sible with a soft cloth dampened with a mild solution of detergent and water NOTE Teflon Valves must be completely flushed with di
8. gital TTL signal An event marker pulse generated each time a valve is switched on is provided at the rea rof the instrument for recording into your acquisition system Valve transitions openedor closed occur at full power to insure rapid response times and are then held in place at less than half power to prevent heat transfer to solutions Features Teflon valves e 6 channels individually controlled Computer controllable Publication 5712 001 REV B www warneronline com s Perfusion Valve Control System Model VC 6T Warner Instruments Operating Directions Setup Components Before beginning setup take inventory of the supplied components You should have valve bracket with included Teflon valves syringe holder support stand e 18 blunt end syringe needles stopcocks 6 Teflon tubing 25ft PE 160 tubing 10ft MPP 6 port manifold VC 6 Valve Controller The VC GT is designed to operate as a stopped flow device where in the valve for each channel is either open allowing solution to flow or is closed In general the shortest response time for delivery of the selected solution will be achieved by keeping the tubing length between the MAN IFOLD and sample as short as possible Publication 5712 001 REV B www warneronline com E Perfusion Valve Control System Model VC 6T Warner Instruments Operating Directions conta Assembly 1 3 5 Begin assembly of the
9. low for a short connection between the manifold and the sample chamber Attach the remaining blunt end syringe needles to one end of each section of Teflon tubing 8 Prepare the MPP manifold to accept the Teflon tubing by first slid ing a short length of PE 160 tubing over the input ports on the MPP manifold 9 Now make a connection between the output port on each Teflon valve and one input port on the MPP manifold Connection should be made using the Teflon tubing described in step 7 Attach the Luer fitting to the valve and the open end to the manifold Publication 5712 001 REV B www warneronline com 2 Perfusion Valve Control System Model VC 6T Warner Instruments Operating Directions conta 10 Run a short length of PE 160 tubing from the output port on the MPP manifold to the input port of sample chamber NOTE If desired you can also run Teflon tubing between the mani fold and your chamber Prepare the manifold to accept the Teflon tubing by first sliding a short length of PE 160 tubing over the out put port on the MPP manifold 11 Finally connect the VALVE MANIFOLD to the VC 6 CONTROLLER using the attached cable AC Conversion ATTENTION PLEASE READ BEFORE APPLYING POWER TO YOUR UNIT The unit has been set to be used with 120 VAC If the VAC needs to be changed to 220 VAC enclosed you will find a kit power cord 1 and fuses 2 to be used to convert the unit from 120 VAC to 220 VAC
10. on Valve Control System Model VC 6T Warner Instruments Warranty The VC 6T Valve Control System is warranted to be free from defects in materials and workmanship for a period of two years from the date of ship ment If a failure occurs within this period we will repair or replace the faulty component s at our discretion This warranty does not cover failure or damage caused by physical abuse or electrical stress e g exceeding specified input limits Shipping charges to the factory are the customer s responsibility Return shipping of the repaired unit will be paid by Warner Instruments Inc Publication 5712 001 REV B www warneronline com Perfusion Valve Control System Model VC 6T Warner Instruments Specifications Specifications Valve Bracket Valves Connection Cable Tubing Reservoirs Reservoir Holder Valve Controller Switch Selection External Input Event Marker Manifold Power Operating Temperature Dimensions H x W x D Weight Shipping Weight Publication 5712 001 REV B Delrin mounts on 3 8 or 1 2 ring stand Teflon 12 VDC 0 25 A to maintain pinch 2 4 meter 8 ft connecting cable terminated with quick disconnects on valve end and 15 pin male D type connector on controller end Teflon 1 8 OD x 1 16 ID tubing 60 cc capacity syringes Delrin Holds six syringes with thumb screws for each reservoir Manual Off or External 5 V TTL compatible BNC Connector Logic lev
11. stilled water after each use Permanent damage will result if saline solution is allowed to crys tallize inside the valve The exterior of this instrument may be cleaned periodically to remove dust grease and other contamination It is not necessary to clean the inside Use a soft cloth dampened with a mild solution of detergent and water and avoid abrasive cleaners Stuck or leaky valves One possibility for a stuck valve is that the valve assembly was not com pletely flushedout at the end of the day While excessive salt build up can result in a stuck valve a moresignificant condition is the potential forma tion of small pits in the valve seals which willresult in a leaky valve A valve bound with salt can be loosened by flushing with warm water to see if thecrystals will dissolve or loosen ETOH may be used for sticky buildups and dilute aceticacid may be used to loosen any mineral deposits With the valve in the open position inject the selected cleaning solution into the flowpath using a 10 cc syringe Inject so solution flows from the input to output ports Repeat as often as necessary usually 3 to 4 washes are sufficient Use an empty syringe to blow airthrough valve to remove any remaining cleaning solution from the valve interior If the valve remains stuck or continues to leak after repeated attempts of this cleaning procedure then the valve should be replaced Publication 5712 001 REV B www warneronline com Perfusi
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