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1. ERO LEVEL potentiometer on the main control panel for a DIGITAL METER reading of 5 units 5 Remove the paper from the plate 6 Position the plate on the area of the plate which should be defined as density 0 0 The viewing screen may be opened 7 Select a neutral filter combination to obtain the largest METER reading less or equal to 4050 units the F command in the control program may be used to get the best setting The neutral filters are selected by rotation of the two rightmost wheels inside the access door at the right side of the bridge If a sufficiently high METER reading cannot be reached the lamp switch on the rack may be switched to MAX position 3 6 SCANNING After the optical and analog systems are calibrated measurements can be made The telescope and viewing screen should be covered when the machine is measuring Scans can be performed by running the proram OPTRO either online or offline or a user application program on the central computer These programs are described in the Computer Program section of the manual OPERATION OF OPTRONICS Table 3 1 Scanning aperture in micron 5x objective Eyepiece Aperture 5x 10x 15x 1 20 x 20 16 x 18 6 7 x 6 7 2 40 x 40 20 x 20 13 3x 13 3 3 60 x 68 3x 30 20 x 29 4 108 x 109 58 x 50 _ 3313 x 33 3 5 30 x 280 15 x 14 19 x 93 6 2 x 280 19 x 149 6 7 x 93 3 x 48 15 x 240 1 x160 8 20 x 48 10 x 24 6 7 x 160 AN K A kok 19x o
2. ON knob on the lower control panel to OUT position 6 Focus the preslit on the viewing screen using the LOWER FOCUS buttons on the main control panel Since the focus is adjusted in very small steps it may take some time before a good focus is reached It is easier to determine the best focus when the plate is positioned on an area with low density i 7 Check that the preslit is positioned close to center of the viewing screen crosShair If this is not the case ask the staff member in charge to adjust the upper microscope body 8 Use the viewing telescope to the left of the screen to center the preslit and the main slit Alignment is accomplished by turning the knobs inside access door on the right side of the bridge The first knob adjust the X axis the next the Y axis and the third the rotation 9 When not in use place the telescope cap on the telescope to avoid that room light can reach the PMT 3 5 ANALOG SYSTEM CALIBRATION The calibration of the analog system must be done with the ILLUMINATION knob on the lower control panel in OUT position The microdensitometer spot should be positioned on the area of the plate which should be defined as density 0 0 or transmission 1 8 e g clear glass plate or plate fog It must be noted that the OPTRONICS does not measure absolute density or transmission values Since the calibration is sensitive to any change in the optical system e g refocusing a new calibration have to be performed
3. P GROSBOL OPTRONICS USER MANUAL JUNE 16 1981 e ESO thera Observ to ESO Libraries IL ML 1 TITLE OPTRONICS User Manual AUTHOR P Grosbol ESO Garching REV June 16 1981 PURPOSE User guide for the OPTRONICS measuring machine ICC C Euler Room no 222 TYPE User OPERATION OF OPTRONICS 3 1 INTRODUCTION Preparing the microdensitometer for operation involves many steps which can be performed in many different ways It is suggested however to follow the steps as outlined in this section Only operations of the microdensitometer is described in this manual while instructions for operation and running the scan control programs are found in the St Program section of the manual Note that all functions of the OPTRONICS machine are controled by the satellite computer Therefore it is essential that the satellite control program is loaded and running If some functions are not working properly try to run the program READY and reload the satellite computer 3 2 STARTUP PROCEDURE 1 Check that the power is on to the system rack and the microdensitome The X Y coordinate display on the rack should be lit 2 Check that the microdensitometer lamp is lit If not call for help 3 Run the control program OPTRO or the user application program on the terminal 4 Switch the ON STANDBY switch to ON by pressing the ON button on the main control panel 5 Check that the stage
4. bjective Eyepiece Aperture i 5x 18 15x 1 10x 19 5 x 5 33 3 3 20 x 20 10 ix 1 6 7x 6 7 3 30x 30 15 x 15 W x10 4 50x 50 25 x 25 17 x 17 5 15 x 146 7 5x Wi 5 x42 6 10 x 148 5 x 7 3 3x42 7 15 x 280 7 5 x 120 5 x 80 8 1 x 280 5 x 12 3 3 x 80 Page 3 5 1 _ 35 25 ad 19 5 2 x 20 7 25 78 70 1207 126 a 2 5 x 2 5 5 7 5 12 5 3 8 2 5 3 8 2 5 x x x x x x x 5 7 5 12 35 35 60 89 5
5. can move in both X and Y direction by pressing the X Y positon buttons on the main control panel If it cannot be moved call for help 3 3 CLOSEDOWN PROCEDURE l Terminate the control program 2 Switch the lamp switch on the system rack to NORMAL position OPERATION OF OPTRONICS Page 3 2 3 4 OPTICAL CALIBRATION The optical calibration and adjustment consist of mounting plate selecting slit and aligning optics The two latter items are not needed when only manual measurements are made 3 4 1 Plate Mounting 1 2 3 4 For the most commonly used plate sizes special adapters are available see Table 2 5 If other plate sizes are used a glass platen shoul be placed in the plate adapter Move the stage in the Y axis towards the main control panel stopping it approximately 10 cm before the end Attach the plate adapter to the Stage and fasten it using the clip and thumbscrew in the corner Each adapter has three reference dowel pins and a clip in one corner Place the plate in its adapter and align against the reference dowels Use the clip to push the plate against the dowles Tighten the thumbscrew on the clip to secure the plate in its adapter 3 4 2 Slit Selection 1 N w 4 Find the best eyepiece objective slit combination yielding the scanning slit desired see Table 3 1 Note that normally the 5x objectives will be mounted and that objectives only will be changed in special cases conta
6. ct staff member in charge Select the lower eyepiece on the lower control panel by turning the MAGNIFICATION knob Install the upper eyepiece in the upper microscope Its magnification must be identical to that chosen for the lower eyepiece A change is done by bringing the microscope to full down position unscrewing the top part taking it out replacing the eyepiece and remounting the top part Select the preslit on the lower control panel using the FIXED APERTURE knob Match the corresponding slit on the slit wheel i e the leftmost wheel inside access door on the rigth side of the brigde The slit number pointing upwards on the slit wheel should be identical to the preslit number selected on one lower control panel 3 4 3 Optical Alignment 1 2 3 Engage field illuminator by turning ILLUMINATION knob on the lower control panel to IN position Focus the plate on the viewing screen by using the focus knob on the upper microscope Check that a good focus is obtained over the entire area OPERATION OF OPTRONICS Page 3 3 which has to be scanned by moving the stage manually to different positions on the plate If this not the case adjust the horizontal level of the stage using the two micrometric screews below the stage one on each side 4 Remove the cape from the telescope to the left of the viewing screen Focus the telescope by pulling a little out The slit aperture should now be sharp 5 Switch the ILLUMINATI
7. when ever such changes are made 3 5 1 Transmission Mode 1 Select transmission mode on the main control panel by pressing the DENS button until the TRANS control lamp is lit 2 Check that the ILLUMINATION knob on the lower control panel is in OUT position 3 Place a dark piece of paper on the plate so that it obscures the light beam Close the viewing screen and set the cap on the telescope 4 Adjust the ZERO LEVEL potentiometer on the main control panel for a DIGITAL METER reading of 5 units 5 Remove the paper from the plate 6 Position the plate on the area of the plate which should be defined as transmission 1 09 The viewing screen may be opened OPERATION OF OPTRONICS Page 3 4 7 Select a neutral filter combination to obtain the lagest METER reading less or equal to 4058 units the F command in the control program may be used to get the best setting The neutral filters are selected by rotating the two rightmost wheels inside the access door at the right side of the bridge If a sufficiently high METER reading cannot be reached the lamp switch on the rack may be switched to MAX position 3 5 2 Density Mode 1 Select density mode by pressing the DENS button until it is lit 2 Check that the ILLUMINATION knob on the lower control panel is in OUT position 3 Place a dark piece of paper on the plate so that it obscures the light beam Close the viewing screen and set the cap on the telescope 4 Adjust the Z

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