operating instructions and system description for the ext
Contents
1. NDI Instruments Electronic for the Life Sciences OPERATING INSTRUCTIONS AND SYSTEM DESCRIPTION FOR THE EXT 10C EXTRACELLULAR AMPLIFIER MODULE FOR EPMS SYSTEMS VERSION 1 5 npi 2005 npi electronic GmbH Hauptstrasse 96 D 71732 Tamm Germany Phone 49 0 7141 601534 Fax 49 0 7141 601266 support npielectronic com http www npielectronic com EXT 10C User Manual Table of Contents Ty alety RES ULATIONS iuuat cus apa deo e a Sors aio pabra s fede asuma raus escasa tras e Dea sumudauE 3 2 JEPMS 07 Modular Plus In5ysteln udio sse quie avene ded ab adive is outied da sddexs suede ddnias 4 2 1 Components of the EPNLES 07 LEIOUSIIE doro ee re tena ae E 4 2 2 General System Description Operation eite eene Res k n devenue Resa ae ruga gue u sae 4 2 9 D VSICTIE CTEOUIICITIE etos ane E nT a RRP ne RI cea ot an 5 ZA TECHNIC INDEM TT 9 Je NG eas M 6 Sal SEXTATOC C OIDOPBDCDS oco mtisitusiete carebit E oa tee 6 3 2 Syse m DESCH PU OM se Liceo at aoo Mna qud on M oa Iv RENE E eade te uide 6 33 Olena FOW W1aS CAI iiece 2ssysaitesiiteminoedupnadind ea ddp pute RR E DERE 6 94 Description ofr the Front Pane heise oC eiu to a netu aetna nes 7 SL SR Qe SETS E ccr et acto cot csc as ea cise search aoa ec eect utd 9 e ere L182 Ved Elements en nce ee ere obe eer er eT eT rte tare etre NE Peo ut itid 9 5 0 CODeLatiOD cays iene emot ECRU e ue atate CUm2. 1 V into 50 Q load selectable gain x100 x1k x10k Output range 12 V into 1 k 2 V into 50 Q load corner frequency 10 Hz 10 turn potentiometer 100 mV analog display for the electrode offset range 30 mV 0 20 pF 10 turn potentiometer front panel 12 HP 60 6 mm x 3U 128 5 mm 7 175 mm deep 115 230 V AC 60 50 Hz fuse 0 8 0 4 A slow 45 60 W dependent on the modules plugged in 19 rackmount cabinet 3U high IU 1 3 4 44 45 mm page 12
3. EXT 10C is designed to be used with the DPA 2F preamplifier filter module from npi which combines additional gain stages with high and lowpass filters 3 3 Signal Flow Diagram The signal is passed through the EXT 10C as shown below Variable DC Electrode B Amplification OUTPUT Headstage connector B Be IN Reference Headstage Differential pe sel p Fatis P T Headstage connector amplifier compensation EENETESENIEOT i pm N Capacity compensation version 1 5 page 6 EXT 10C User Manual 3 4 Description of the Front Panel 6 I oO Qo POTENTIAL x10mV 4 7 o pn 3 AP A HEADSTAGE Figure 2 EXT 10C front panel view In the following description of the front panel elements each element has a number that 1s related to that 1n Figure 2 The number 1s followed by the name in uppercase letters written on the front panel and the type of the element in lowercase letters Then a short description of the element 1s given 1 HEADSTAGE connector The HEADSTAGE cable is connected to the unit at an 8 pin connector in the center of the module BNC connector providing the amplified signal DC coupled The amplification factor is set by the GAIN DC switch 3 version 1 5 page 7 EXT 10C User Manual 3 GAIN DC switch Switch to set the GAIN of the outgoing DC signal Three GAIN factors are available x10 x100 or x1k 4 C COMP potentiometer Control for comp
4. amp Grantyn R eds 1992 Practical Electrophysiological Methods Wiley Liss New York J Lalley P M A K Moschovakis and U Windhorst 1999 Electrical Activity of Individual Neurons In Situ Extra and Intracellular Recording in U Windhorst and H Johansson eds Modern Techniques in Neuroscience Research Springer Berlin New York LI M ller Ch M 1992 Extra and Intracellular Recording in the Slice in Kettenmann H amp Grantyn R eds Practical Electro physiological Methods Wiley Liss New York I Ogden D ed 1992 Microelectrode Techniques The Plymouth Workshop Handbook Second Edition The Company of Biologists Ltd Cambridge I Seidenbecher T and H C Pape 2001 Contribution of intralaminar thalamic nuclei to spike and wave discharges during spontaneous seizures in a genetic rat model of absence epilepsy European Journal of Neuroscience Vol 13 1537 1546 L Windhorst U and H Johansson eds 1999 Modern Techniques in Neuroscience Research Springer Berlin Heidelberg New York version 1 5 page 11 5 Technical Data Differential Input Input resistance Output DC Output AC Offset compensation Potential monitor Capacity compensation Size EPMS 07 SYSTEM Power Requirements Dimensions version 1 5 EXT 10C User Manual CMR gt 90 dB at 1 kHz tested with 0 input resistance gt 10 Q range 1 V selectable gain x10 x100 x1k Output range 12 V into 1 kQ2
5. etu ru Cuesta CDD Ue phot Ins mf 9 Ac NAV C Cun m 11 De Technical Datacsusicnee v EE rr eese a Lei E ute eee de Pon 12 version 1 5 page 2 EXT 10C User Manual 1 Safety Regulations VERY IMPORTANT Instruments and components supplied by npi electronic are NOT intended for clinical use or medical purposes e g for diagnosis or treatment of humans or for any other life supporting system npi electronic disclaims any warranties for such purpose Equipment supplied by npi electronic must be operated only by selected trained and adequately instructed personnel For details please consult the GENERAL TERMS OF DELIVERY AND CONDITIONS OF BUSINESS of npi electronic D 71732 Tamm Germany 1 2 3 4 5 GENERAL This system is designed for use in scientific laboratories and must be operated only by trained staff General safety regulations for operating electrical devices should be followed AC MAINS CONNECTION While working with the npi systems always adhere to the appropriate safety measures for handling electronic devices Before using any device please read manuals and instructions carefully The device is to be operated only at 115 230 Volt 60 50 Hz AC Please check for appropriate line voltage before connecting any system to mains Always use a three wire line cord and a mains power plug with a protection contact connected to ground protective earth Before opening the cabinet unplug t
6. signals in electrophysiology see Figure 1 The system is housed in a 19 rackmount cabinet 3U containing a power supply and has room for up to 7 plug in units The plug in units are connected to power by a bus at the rear panel The plug in units must be kept in position by four screws M 2 5 x 10 The screws are important not only for mechanical stability but also for proper electrical connection to the system housing Free area must be protected with covers In order to avoid induction of electromagnetic noise the power supply unit the power switch and the fuse are located at the rear of the housing B4 HP 7 skancdord seis 35 MP 3o J2 ME peor duet 28 HP j pat D uc CHANEL A Figure 1 Example of a measurement system located in an EPMS housing with two channel iontophoresis and balance module MVCS 02 MVCC 02 bridge amplifier module BRAMP 01 R and an extracellular amplifier module EXT 01C version 1 5 page 4 EXT 10C User Manual 2 3 System Grounding The 19 cabinet is grounded by the power cable through the ground pin of the mains connector protective earth see chapter 1 In order to avoid ground loops the internal ground is isolated from the protective earth The internal ground is used on the BNC connectors or GROUND plugs of the modules that are inserted into the EPMS 07 housing The internal ground and mains ground protective earth can be connected by a wire using the ground plugs on t
7. D jack REF a Holding bar differential measurement configuration B Experimental chamber REF Ag Aacl electrode pellet Electrode holder BNC jack Per SUBCLICK Ground connector ggg GND Holding bar single ended measurement configuration Figure 4 headstage connections A differential measurement B single ended measurement version 1 5 page 10 EXT 10C User Manual 4 Literature Ld Barmashenko G Eysel U T amp Mittmann T 2003 Changes in intracellular calcium transients and LTP in the surround of visual cortex lesions in rats Brain Res 990 120 128 L1 Boulton A A Baker G B amp Vanderwolf C H eds 1990 Neurophysiological Techniques Basic Methods and Concepts Humana Press Clifton New Jersey L Huemmeke M Eysel U T amp Mittmann T 2002 Metabotropic glutamate receptors mediate expression of LTP in slices of rat visual cortex Eur J Neurosci 15 1641 1645 I Huemmeke M Eysel U T amp Mittmann T 2004 Lesion induced enhancement of LTP in rat visual cortex is mediated by NMDA receptors containing the NR2B subunit J Physiol 559 875 882 L1 Schulz D Huston J P Jezek K Haas H L Roth Harer A Selbach O amp Luhmann H J 2002 Water maze performance exploratory activity inhibitory avoidance and hippocampal plasticity in aged superior and inferior learners Eur J Neurosci 16 2175 2185 I Kettenmann H
8. ensation of stray capacities of the microelectrode 10 turn potentiometer range 0 20 pF Due to the positive feedback circuit overcompensation of the capacity will lead to oscillations which may damage the cell Behn The analog POTENTIAL monitor displays the offset in the range of 30 mV JT TE and is used for optimal cancellation of the OFFSET 6 OVER OVER LEDs Pa LEDs that indicate if the amplifier 10 below it s positive or negative limit 10 V The linear range of the amplifier is 12 V 7 OFFSET potentiometer 10 turn potentiometer to compensate for the OFFSET of a DC signal Note Position 5 on the OFFSET control corresponds to 0 mV offset 8 GAIN AC switch Switch to set the GAIN of the outgoing AC signal Three GAIN factors are available x100 x1k or x10k 9 AC OUTPUT connector BNC connector providing the amplified signal AC coupled The amplification factor is set by switch 8 Switch to set the NOTCH filter 50 Hz 60 Hz or to bypass the NOTCH filter switch position OFF version 1 5 page 8 EXT 10C User Manual 3 5 Headstage Figure 3 electrode holder optional and headstage of the EXT 10C Headstage Elements BNC connector for the electrode holder REF reference electrode connector GND ground connector holding bar A WN On request Pg can be implemented using mm or 2 mm banana jacks or using SMC SUBCLIC connectors 3 6 Operation Extracellular measure
9. he instrument Unplug the instrument when replacing the fuse or changing line voltage Replace fuse only with an appropriate specified type STATIC ELECTRICITY Electronic equipment is sensitive to static discharges Some devices such as sensor inputs are equipped with very sensitive FET amplifiers which can be damaged by electrostatic charge and must therefore be handled with care Electrostatic discharge can be avoided by touching a grounded metal surface when changing or adjusting sensors Always turn power off when adding or removing modules connecting or disconnecting sensors headstages or other components from the instrument or 19 cabinet TEMPERATURE DRIFT WARM UP TIME All analog electronic systems are sensitive to temperature changes Therefore all electronic instruments containing analog circuits should be used only in a warmed up condition 1 e after internal temperature has reached steady state values In most cases a warm up period of 20 30 minutes is sufficient HANDLING Please protect the device from moisture heat radiation and corrosive chemicals version 1 5 page 3 EXT 10C User Manual 2 EPMS 07 Modular Plug In System 2 1 Components of the EPMS 07 Housing The following items are shipped with the EPMS 07 housing X EPMS 07 cabinet VK Power cord V Fuse 0 8 0 4A slow X Front covers 2 2 General System Description Operation The npi EPMS 07 is a modular system for processing of bioelectrical
10. he rear panel of the instrument It is not possible to predict whether measurements will be less or more noisy with the internal ground and mains ground connected We recommend that you try both arrangements to determine the best configuration 2 4 Technical Data 19 rackmount cabinet 3U high 1U 1 3 4 44 45 mm for up to 7 plug in units Power supply 115 230 Volts AC 60 50 Hz fuse 0 8 0 4 A slow 45 60 W version 1 5 page 5 EXT 10C User Manual 3 EXT 10C 3 41 EXT 10C Components The following items are shipped with the EXT 10C system V Amplifier module for the EPMS 07 system V GND 2 6 mm banana plug and REF SMB connectors for headstage X Headstage V User manual 3 2 System Description The EXT 10C was designed for performing low noise recordings of small extracellular signals in slices or in in vivo preparations using fine tipped glass or metal microelectrodes The system consists of a module for the npi EPMS 07 modular system and a small headstage with a holding bar The EXT 10C has a differential input with high input resistance for minimizing noise and the incoming signal can be compensated for offset and capacity of the microelectrode by the two potentiometers OFFSET and C COMP An analog balance monitor makes the control of offsets easy The recorded signal can be obtained either AC with GAIN of 100 Ik or 10k or DC with GAIN of 10 100 or Ik An optional NOTCH filter eliminates 50 60 Hz noise The
11. ments are mostly done in slices or in vivo where distortions of the signal caused by other instruments and the animal itself are very common Additionally extracellular signals are very small and have to be amplified enormously The drawback is that noise 1s amplified as well Therefore the headstage of the EXT 10C is equipped with a differential input that minimizes noise pick up Differential means that the signal for the amplifier is the difference between the positive Pg and negative REF input of the headstage This results in canceling of all signals which both electrodes record e g noise For differential measurements both inputs of the headstage REF and Pg are connected to microelectrodes using cables with grounded enclosure or electrode holders Pg 1s connected to the measuring electrode and REF to the reference electrode The experimental chamber 1s grounded by an Ag AgCl pellet or an AGAR bridge connected to GND of the headstage see Figure 4 If differential measurement is not required single ended measurement configuration see Figure 4 the REF input must be connected to ground GND The amplifier is in an undefined state if the REF is left open and can go into saturation making reliable measurements impossible version 1 5 page 9 EXT 10C User Manual A Experimental chamber REF Ag AgCI electrode pellet Electrode holder BNC jack Pe SUBCLICK X Ground connector jack REF N
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